Tgp chi Khoa hoc - Cong nghe Thuy sdn SS 3/2^l4
KET QUA NGHIEN CQU D A O TAO SAU DAI HOC
ANH Hl/OfNG CtlATHlTC AN BO SUNG FRUCTO-OLIGOSACCHARU)E (FOS) LEN TANG TRlTOfNG, TY LE SONG VA CAC T H 6 N G SO STNH LY
CUA TOM SU {Penaeus monodon Fabricius, 1798)
EFFECTS OF FRUCTO-OLIGOSACCHARITE ON GROWTH, SURVIVAL RATE AND PHYSIOLOGICAL PARAMETERS OF BLACK TIGER SHRIMP
(Penaeus monodon Fabricius, 1798)
Dang Tran Tu Tram', Luc Minh Difp', Huynh Minh San^
Ngay nh^n bai. 30/10/2013; NgSy phan bien thong qua: 17/02/2014, Ngay duyet dang: 13/8/2014 T O M T A T
TTii nghidm dupc lien hdnh trong 90 ngdy tai Tram thuc nghiem nuoi trong thtiy sdn, flen Hdi duang hoc ven 4 nghiem thirc thuc an co bo sung cdc ham luang FOS khac nhau Id 0,1%, 0.2%, 0,4%, 0,8% vd nghidm thuc doi chimg Midng bo sung FOS. Xdc dinh tyle song, tang Iruang cua torn sau 30, 60 va 90 ngay nuoi. Cdc chi so sinh ly (TMl: chi so ca thft, HSI: chi so gan luy, HM. dg dm gan tiff, TM: dp dm ca thfl) duac xdc dinh khi kit thuc thi nghiem. Kit qud cho thdy, loc dg tang tru&ng d^c trung ve khoi luang (SRGw) cao nhdt & nghiem thuc thtrc dn co bo sung 0.2% FOS (dgt 3,70 ± 0,045 %/ngdy) vd thdp nhdi & nghiem thiic thiccdn CO bo sung 0,8% FOS (dgt 3,51 ± 0,045 %/ngdy). Muc lang khoi li^ng tnmg binh hdng ludn (AWG) cao nhat & nghiem thuc 0,2% FOS (0,45 ± 0,017 g/tudn), thap nhat & nghi4m thuc 0,8% FOS (0,38 ±0,015 g/tudn. Tom cho dn thUc an co bd sung 0,4% FOS co HSlw vd TMIw cao nhdt va ihdp nhdt & torn khong dugc bo sung FOS vdo thitc an (p < 0,05) nhung khong co su khac nhau ve chi sd stnh ly khac (TM, HM, HSId vd TMId) giUa cdc nhom torn cho dn cdc logi thuc dn co bo sung cdc hdm luang FOS khac nhau Kit qud nghiin cuu cho thdy bo sung 0,2 - 0,4% FOS vdo thiic an cdi thien site khoe ctia tom sti nuoi
Tirkhoa ldm su (P. monodon),prebiotic.fructo-oligosaccharide (FOS) ABSTRACT
The experiment was conducted for 90 days at Experimental Station, Institute of Oceanography with treatments:
0.1% FOS, 0 2% FOS. 0 4% FOS. 0 8% FOS and control (0 0% FOS) The growth rate and survival were determined after 30. 60 and 90 days of study. Some physiological parameters (TMLtail muscle index, HSI: hepatosomatic index, HM:
hepatopancreas moisture, and TM: tail muscle moisture) were determined at the end of the experiment. The results showed that specific growth rate (SRGw) was highest in treatment ofO % FOS (3.70 ± 0.045 % /day) and lowest in treatment of 0.8% FOS (3.51 ± 0.045 %/day) The average weight (AWG) of shrimp was gained maximum value (0 45 ± 0.017 g/weeh) in treatment of 0 2% FOS and lowest value (0 38 ± 0.015 g/week) in treatinent of 0.8% FOS. The HSIw of shrimps fed diets which supplemented with 0.4 % FOS were highest. However, the TMIw showed a lowest value when FOS is added to food (p < 0,05) There were no significant differences in other physiological indicators (TM, HM and TMId HSId) between groups when they fed wilh different concentrations of FOS Results showed thai the feed which supplemented of 0.2 -0 4%
FOS improved the health of shrimp farming.
Keywords • shrimp, prebiotic, fincio-oligosaccharlde (FOS)
' Dgng Tr^n TO Tram: Cao hgc Nuoi trSng thuy san 2011 - Trudng Dgi hgc Nha Trang
^ TS. Lgc Minh Di$p' Vien Nuoi trong IhOy san - Tm-dng Dai hgc Nha Trang
^ TS. HuS'nh Minh Sang: Vi§n HSi dU'O'ng hgc Nha Trang
190 • TRUONG OAI HOC NHA TRANG
rap chi Khoa hoc - Cong nghe Thuy sdn So 3/2014 LOATVAND^
Sy dgng khang sinh lieu iu-crng thip trong nu6i tring ttiHy san kfch thich sy tang tn/crng, tiing hi^u qui sy dyng thijc an va ty !§ song cua c i c d6i typ-ng nu6i (Rosen, 1996) nhu'ng cung kich thich sy phit triin cua vi khuin khing thuoc (Gene v i cs, 2007).
Lu$t v4 cam ho^c hgn c^e si> dung khang sinh trong nudi tr6ng thuy san da khuyen khich cic nghiSn ci>u nham tim ra c i c chat bo sung v i o thi>c i n lim ting cu-frng sijc khoe cua v^t nu6i, co the thay th4 mOt phan hoac toan bp khing sinh tnang nufii thuy sin theo hyang thin thien vai moi t r u i n g sinh thii (Gatlin v i cs, 2006). Cac chit bo sung ap dung trong nuoi trong thuy s i n duwc chia thinh 2 nhom l i chit b i sung dinh dirang, cai thi§n mien djch v i chit kich thich hp mien dich phu thupc v i o ca che tic dyng cOa chung. Mgt nhom cac chat thupc chit kfch thi'ch mien djch da chijng to hieu qua trong vipc nudi gia sue, gia cam v i cac doi tu-yng nuoi thuy san l i prebiotic (Sang v i Fotedar, 2011).
Prebiobc duwc dinh nghla "la thinh phan lin men co chpn lpc lam thay doi tinh d i e tryng ve thinh phin v i hoat dpng cua he vl stnh v i t dycmg rupt nham tang sue khoe cho v i t diCi mpt cich giin tiep' (Gibson va cs, 2004). Thong qua v i ^ cung c i p dinh duwng mOl cich co chpn Ipc cho mpt hoac mot so vi stnh vat trong dyang njfit, prebiotic lim thay dot co chpn Ipc he vi sinh v i t du'&ng ru$t ciJa v i t chu (Teitelbaum v i Walker, 2002). Trong cic logi prebiotic thong dgng d u ^ ^ diJng trong nong nghipp va thuy s i n thi Inulin, Fructo-oligosacchandes (FOS), Galacto-oligosaccharides (GOS) v i Mannan-oligosaccharide (MOS) dang du>yc chu y nhiSu n h i t
Hipn nay, co nhiiu n g h i ^ cmj dup'c thyc hi§n
^ danh g i i hipu q u i cua FOS tren cac loii c i nuoi nhy c i h6i (Rehulka va cs, 2011), ca tam (Akrami va cs, 2009), ca ban (Mahious va cs, 2006). c i Hdng My (Ai v i cs, 2011), ca hoi Dgi Tay (Grisdale-Helland v i cs, 2008), ca t i m Sibena (Mahious, 2006). Tuy nhten, c^ya c6 ket qua nghien ciJu nao ve hipu qua cua FOS trong nuoi giip x i c noi chung v i tfim sCi n6i rieng. Do do nghien cuu nay buac d i u cung c i p ca sb khoa hpc dio vi^c sCr dyng FOS b6 sung v i o flit>c i n nhim tang cy6ng sue khoe cua tom su gfip phin hgn che su' dyng khing stnh v i djnh hyfi'ng ky thuit nufii theo hyang b^n vung, thin thi^n vdi mfii tryfi-ng.
II. PHl/ONG P H A P NGHIEN Cl/U 1.V$tli$u nghien CIFU
1.1. Thdn gian va dja diim nghien cOv Nghien cu-u niy duprc thyc hi^n tir thing 9 nim 2012 den thing 12 nim 2012 tgi Trgm thyc
nghipm Nuoi tring thuy san - \fi$n H i i du'ong hpc Nha Trang.
1.2. H$ thdng bi nudi
G i m 15 be composite (100 x 50 x 100 cm, 500 L) dup'c d$t trong nha cfi m i i che. Ny&c btin duyc bam v i o h# thing be lang v i be lpc sau dfi cung c i p tn,fc tiip cho b i nufii. M6i be thi nghidm flyp-c b i tri 1 h§ thing Ipc sinh hpc tuin hoin dpc l§p v i dam b i o syc khi tiin tue trong suit qui trinh nufli.
1.3. NguSn torn thi nghiem
Tfim sCi giong (P15) duac mua tir Trung t i m giing thuy san cua Vien Nghien cuu Nuoi trong thuy s i n Ml v i dyprc yang nuoi tgi Trgm thyc nghipm Nuoi trong thiiy sin -Vipn Hai duang hpc. Tom giing dyp-c uang trong 30 ngay, hing ngay cho i n 4 lan (7 gio', 11 gifi-, 14 gia, 17 gio-) bing thuc an cua Lansy v i Uni President tiiy theo ngiy tuii d i n 3 - 4 cm (khil lu(7ng frung binh 0,21 ± 0,007 g) thi tiin hinh thi nghidm.
1.4. Chuan bj thCrc Sn thi nghiem
Thyc i n goc: su dung thuc i n thuang mai cua UP (Uni President) (Nuri N312: 40% dgm, 4% chit beo, 11% dp i m , 13% tro, 3% xa thfi) lim thuc i n d i i Chung trong thi nghiem.
Chuan bj thi>c i n thi nghipm: Thi>c i n UP dup'c nghiin nat va bo sung FOS (Anhui Minmetals Devetopment Imp. & Exp. Co., Ltd, China, 95% Purify) vfi'i him lyp'ng 0 g/kg (DC), 1 g^g (F0S1), 2 g/kg (F0S2), 4 g/kg (F0S4) va 8 g/kg (F0S8) d l dup-c cic hon hpp thO'c i n tom c6 b i sung 0,0; 0,1; 0,2; 0,4 v i 0,8% FOS. Thuc an su dyng trong thi nghipm dugrc che biin tgi Phong Thi nghiem - 86 mon Dinh dufl'ng, TnTtyng Dgi hpc Nha Trang. Theo cac byfic sau:
Phii trfin nguyin ti^u -> Trpn kho -* Tgo hon hyp d6o -» Tgo sai —. Hap 5 phut - * Siy cr 40°C, 12 gifi' - * Tgo vien - * Bio quin.
1.5. Phirong phap bo tri thi nghidm Tfim su dup'c t h i nuoi of 15 t)e thi nghi$m vfi'i m i t dfi ban d i u la 30 con/be, khii lup'ng tom: 0,21 ± 0,007 g/con. Mot nghiem ttiuc thuc an cfi 3 b i lip, tflm d u p t cho i n 1 trong 5 cflng Ihuc thiJc an cfi b i sung h i m lyi^ng FOS khac nhau v i dup'c nuoi trong 90 ngiy. Tfim duyc cho an 2 lin/ngay v i o Itic 8:00 gifl" v i 17:00 gifl' vai lieu luang 5% khoi luyng thin.
Djnh ky v$ sinh thuc i n thira v i chit thii cua tflm tryfi'c khi cho i n . C i c s i thong s i mfii trtpfi'ng (nhipt dfl, pH, dfi mgn) dyyc kiem fra djnh ky hing ngiy.
Ty lfi sing v i toe dfl tang tnfdng cua tfim dypc dinh gia sau 30, 60 v i 90 ngiy nuot. Cac Uifing s i sinh ly ciia tflm dugc danh g i i sau 90 ngiy nufli.
TRUONG DAI HOC NHA TRANG . 1 9 1
Tap chi Khoa hpc - Cong ngh$ Thuy sdn So 3/2014
1.6. Thu th$p, phSn tich v i xi> /y s i li$u - C i c y i u t i moi tryfi'ng
Nhi^t dfl: do bing nhiit k i cfi dfl chinh xic d i n 0.5"C;
pH: do bing miy do pH (Hanna) cfl dfl chinh xac din 0,1;
DQ m$n: do bing khuc xg k i Atago co dfi chfnh xic 1%a.
- Ty Ip sing ciia tfim trong tirng b^ thf nghifim dup'c x i c djnh theo cflng thuc S = 100"(n/n(,), trong dfi: n, l i s i tfim & thfi'i diim t, n^ l i so tfim d thfi'i diim bit d i u thf nghifim.
- Khii lup'ng ciia tfim dyp'c x i c djnh bang c i n difin ti> SHIIVIADZU AW 220 (LabCommerce Inc, USA) cfi dfi chinh x i c 0,01 g, khii lup'ng tfim diing de xac djnh t i c dfi tang t r u i n g tuang d i i (SRG) v i ting tari'ng binh quin hing tuin (AWG).
SRG=100*(lnWf-lnWo)/t AWG (g/tuan) = (Wf - Wo)/wk Trong dfl: Wf: khii lup'ng cua tflm tgi tho'i diem t;
Wo: khii lup'ng cua tflm tgi tho'i diem bat dau thi nghidm;
t: s i ngiy;
Wk: so tuin.
- Chi tieu sinh ly ciia tflm nuoi ducrc x i c djnh theo phuang phap duyc mfl t i bo-i Sang v i Fotedar (2004) sau khi ket thiic thf nghifim. Mfli nghifim thirc thu 3 c i t h i tfim, giai phiu liy tuyin gan tyy v i ca thit v i x i c ^ n h chi s i gan tyy tuai (HSiw), cht so ca thit tuoi (TMIvtr). Cac chf tieu niy dup'c xac djnh theo cong thuc sau:
HSIw = Wh,x100/W TMIw = Wt,x100/W Trong dfi:
Wh,: khii lup'ng tyci ciia tuyin gan tuy (g);
Wt,: khii lygng tuai cCia ca thtt (g);
W: khii lygng tflm (g).
Sau do tuyen gan tyy va ca thjt cua tfim dugc say d i n khoi lugng khflng d i i a 105 "C trong 24 gifi' v i x i c djnh dp i m cua gan tyy (HM%), do am cua ca thjt (TM%), chi so gan tyy khfl (HSId) v i chi cy dufii khfl (TMd) theo cac cflng thirc:
HM % = 100 X (Wh, - Wh;)/ Wh, TM % = 100 X (Wt, - Wtj) /Wt,
HSId=Wh2x100/W TMId=Wt^x100AV Trong dfl:
Wh,: khii lugng tuai cua tuyen gan tuy (g);
Wh^: khii lugng khfl cua tuyen gan tyy (g);
Wt,: khii lugng tyai cQa co thjt (g);
Wt^: khoi lugng khfl ciia ca thjt (g).
Sir dyng phin mem Microsoft Office Excel 2003 va SPSS 16.0 de xu ly so lifiu. G i i trj so lipu dugc
t h i hifin CT dgng tmng binh ± SE. Su dyng phep phin tich phuang sai mfit y i u t i (one-wray ANOVA) kiim ^nh sy khic nhau ciia c i c nhfim g i i trj. SCr dyng phep so sinh sy sai khic eiia c i c g i i trj tmng binh sau phin tfch phuang sai (Post Hoc Test) bang phuang phip kiim djnh Least significant difference (LSD). Khic nhau giij-a eic gia trj dugc x i c djnh co y nghla d m(rc p < 0,05.
III. K f T QUA NGHIEN CLTU VA T H A O L U A N 1. Mot so chl t l i u moi tnrd'ng trong tho'i gian thi nghidm
Cie y i u t i nhifit dfi, dfl man va pH dugc kiem tra trong thfi'i gian thf nghifim d i u i ngudng dao dpng thich hgp eho sy sinh fruang binh thufi'ng ciia tfim sCi nufii (bing 1), cic b i nufii frong dleu kifin cham sflc nhu nhau nen khflng co sy khic nhau ve cic thfing s i mfii tryfi'ng giua c i c b i thi nghifim.
Bang 1. Mgt so y i u t i mfil tryfi-ng trong thcfi gian thi nghipm Yeu to m6l tnrimg
pH
Nhiet do (°C) S^ng Chieu Dp man {%o)
Gid tri 7,7 - 8,2 2 7 - 2 9 (27,76 ±0,59) 28 - 30 (28.46 ± 0,55) 32-34 (32,33 ±1,72) 2. Anh huvng cua FOS den ty le s i n g cua tom sii
Sau 30 ngiy nufii, t^ lfi sing cua tfim fl' cac nghipm thuc thiJC an dgt r i t eao v i tuang dii diu nhau, cao nhat 6' F0S8 (100%), ke den la F0S1 (97,78 ± 1,11%), F0S2 (96,67 ± 3,33%), F0S4 (95,56 ± 2,94%) v i thap nhat o' d i i chiJng (94,441 2,94%) nhung d i n 60 ngiy nufli thi t^ lfi sing cua tfim Igi cao nhit & nghipm thuc FOS4 (94,45 ± 2,22%) v i thip nhit 6- F0S1 (78,89 ± 13,89%) v i xu t h i nay keo d i i d i n 90 ngay nufli, ty Ip sing dat cao nhat d F0S4 (90,00 ± 5,09%), F0S8 (87,88 ± 7,29%), F0S2 (82,22 ± 9,09%), doi chung (80,00
± 10,18%) va thip nhit b FOS1 (71.11 ± 14,70%).
Mac dil t^ lfi sing cila tflm sii frong suit qui trinh nufii 6' nghipm thuc F0S4 co xu hufl-ng cao han cic mirc bo sung khic va on d|nh trang suit thfi'i gian tiin hinh thi nghipm nhung kit qua thf nghifim cung cho thiy khfing cfi sy sai khac cfi ^ nghTa thing kfi ve ty lfi song giO'a tom dugc cho i n bing thiPc an doi chLPng vfi'i tfim cho an bang thuc an cfl bo sung FOS vai cac him lugng khac nhau (hinh 1). Diiu nay dugc giit thfch l i do dieu kipn mfli tryfl-ng v i dinh duo'ng trong dieu kien thi nghipm tuang dii tit, phil hgp vfi'i nhu cau phit then ciia tflm sii nuoi (Sang va cs, 2010a, 2010b, 2011).
192 • TRUONG OAI HOC NHA TRANG
Tap chi Khoa hoc - Cong nghe Thuy san So 3/2014
100.00
g TS,00
1
50 00 30
^ r
—P~^^—-^,
80 90 Th^l fllin Ihl nglil4m(nsly)
—
Hinh 1. TJ 1^ song ciia t6m sii trong cic nghifm thiix: thl nghifm 3. Anh huwng cua FOS den tang tivd-ng cua
tfim su
Tang trufl'ng ve khii tugng cCia tflm (bang 2) t^o thiy, sau 60 ngiy nufli khflng cfi sai khic ding ke giiJa tflm doi chirng v i tfim dugc cho an
thirc i n cfl b i sung FOS. Sau dfi, khii lygng tflm fl' c i c nghipm thuc cfi bo sung FOS diu tang ding k l , dgt cao nhat fl" nghifim thi>c F0S2 (dgt 5,67 ± 0,23 g) va thip nhit 6' nghipm thCfc F0S8 (4,77 ± 0,18 g).
NQnh 2. Tang trao'ng cua tom sii trong cic nghidm thirc thf nghifm Tic dfl tang trirong dgc tryng ve khoi lygng
SRGw cao nhit a F0S2 (3,70 ± 0,045%/ngiy) v i Blip nhit 0' FOS8 (3.51 ± 0,045 %/ngay). Muc tang khii fugng tmng binh hing tuin AWG cao nhit cung 6 F0S2 (0.45 ± 0,017 g/tuan) v i thap nhit fl- FOS8 (0,38 ± 0,015 g/tuan). K i t q u i thi nghifim cho thiy, sau 90 ngiy nufli, cac thflng so ve khoi lugng tflm,
t i c dfi tang trufl'ng ve khoi lugng cua tflm giira cic nghipm thu'c thuc an khac nhau dfiu khfing cfi sai khac cfi y nghla thing ke. Tuy nhien, eic thflng so v i t i n g tru'fi'ng v i ty le sing ciia tflm nufli khi b i sung FOS vfl't him lugng dao dpng tti" 0,2% - 0,4%
vao thirc an cfi xu hyfi'ng cao han tfim nufii bing c i c cfing thuc thue i n khic (bang 2), Bing 2. Cic thong s i tang tryfi'ng sau 90 ngiy nufii Ngtiigm Hi Ac
BC FOS1 F0S2 F0S4 FOS8
Tli6ne s i W(S)
5,06 ± 0,02 6,23 ± 0,87 5.67 ± 0,23 4.92 ± 0,07 4.77 ±0.18
SRG (%) 3.58 ± 0.003 3.58 ±0,191 3,70 ± 0.045 3,54 ±0.015 3.51 ± 0,045
AWG (grtu4n) 0.41 ± 0,003 0.42 ± 0,072 0,45 ± 0.017 0.39 ±0,006 0.38 ±0.015 Hifiu qua khfing ro ring v i ting trufl'ng v i
t? lfi sing khi bo sung FOS v i o thirc an cung dugc quan s i t thay 6" mfit s6 d i i tugng nufii thuy sin khac nhu tren c i hii (Oncorhynchus mykis
Rehulka et al, 2011) v i h i i sam (Apostichopus japonicus) (Yancui, 2011), Kel qui tuong ty khi nghifin ci>u trfin tflm chan trang trong hfi thing nufii tuin hoin trong 6 tuin vfi'i cic muc bo sung 0; 0,1 v i 0,8%
TRUONG DAI HOC NHA TRANG • 193
Tap chi Khoa hoc - Cong nghe Thdy sdn S6 3/2014 frutooligosaccharide chuii ngin (scFOS) cOng cho
thiy khfing cfi sy c i i thifin v i hfi s i chuyin d i i thiic an cOng nhu ty lfi sing cda tflm nufli (Li., P v i cs, 2007). Tuy nhien, nhiiu nghifin cuu khac lgi cho ring bo sung FOS v i o thirc i n da c i i thipn ding ke ty le sing v i SGR cua c i nhy ca diy (Rutilus mW/usSoleimani etal, 2012). D i i vd\ tflm chan tring, mpt nghten cuu khic cho thiy, b i sung 1,6 g FOB/ kg thue an da cii thifin SGR (Zhigang, 2007). Theo t i c gia niy thi khi b i sung 0,4% FOS chuoi ngin (sFOS) thi tfim chin tring cfl toe dp tang trufl'ng cao han, he so thuc i n thip han v i eho ring khoing b i sung FOS cfi t h i xem xfit khoing 0,4% - 1,6%
va ket lufin b i sung 0,4% FOS la phu hgp nhit cho tflm chan tring, Sy khic nhau ve hipu qua ciJa FOS din ting tryfi'ng v i t;^ lfi sing cOa c i c doi tugng nufii khic nhau l i khic nhau. Diiu niy cfi t h i do nhu cau ve mgt dinh dyflng cua eac doi tugng nufii khac nhau, tinh trgng si>c khoe v i tinh trgng hogt dfing cua hfi vi sinh vfit cfi Igi trong duo'ng mot eua cic d i i tugng nufli khic nhau eung nhu dieu kifin mfli trufl'ng nufli khic nhau.
4. Anh h(F6ng cda FOS den c i c chi s6 sinh ly cOa tfim su
C i c chi tieu sinh ly nhu chi s i tuyin gan tijy khfi v i yfli, chi so ca thjt khfl v i vrCrt v i h i m lygng nufl'c da dugc su- dung d i dinh g i i tinh trgng stre khfie cOa c i c loii giap x i c (Jussila, 1997b). Chi s i tuyin gan tyy cao eiing vfi'i h i m lugng nudc thap l i chf thj cOa tinh trang sO-c khfie t i t cua giip xac Trong khi nhung bieu hifin v i tang tnjdng v i ty lfi sing chua t h i hipn thi c i c chi so sinh ly cfi t h i diing d i dinh g i i sue khoe cua giip x i c (Jussila, ig97b, Mannonen v i Henttcnen, 1995, McClain, 1995).
C i c chi s i sinh ly nhy chi s i gan tyy (HSI), chi s i co thjt (TMl), dfl i m gan tuy (HM) v i dfl i m ca thjt (TM) l i chf thj ciia SCHC khoe va tinh trgng sinh ly cilia tfim.
Sau 90 ngiy nufli chi s i gan tyy ufll (HSIw) cua ciia tflm an thi>c an F0S4 l i cao nhit (4,79 ± 0,47'') v i thip nhit fl nghipm thirc tflm an thirc 3n DC (3,65 ± 0,21«). Tuy nhifin kit q u i cho friiy khflng cfl sy khic nhau ve chi s i sinh ly khic (TM%, HM%, HSId v i TMId) gii>a c i c cic nghifim Uiue cfl bo sung cic him lugng FOS khae nhau (bing 3).
B i n g 3. Mpt so chi tieu sinh ly ciJa tfim sO nuoi sau 90 ngay thi nghiem Chj so sinti ly (%)
TMiw TMId HSIw HSid TM HM
Ngtii^m tliipc DC
43.73 ± 1.36"
11.92 ± 0.48' 3,65 ±0.21- 1,04 ±0.15' 75.05 ± 0,29' 71.80 ±2.82"
FDS1 45.86 ±2.12«
10,99 ±0.73"
3.86 ± 0,24"
1.01 ±0.13- 76,08 ± 0,58' 73,96 ±1,88"
F0S2 47,30 ±1,04"
12,02 ±0,40' 3,74 ± 0,34'"
1,15 ±0,23"
74,60 ± 0,54- 69,89 ± 3,93-
FOS4 48,99 ± 0,82- 12,51 ±0,42- 4,79 ± 0,47' 1,35 ±0,09' 74,46 ± 0,64"
71,54 ±1,35"
F0S8 47,21 ± 1.39- 11.69 ±0,22- 4.18±0,23*
1,26±0,W 75.21 ± 0,45' 69.96 ±0.16' So li^u cung hing cd cdc chir cdi khAc nhau thi hi^n sai khic
K i t q u i v i cic chi so sinh ly cho thiy khflng cfl sai khic cfl y nghTa thing kfi v i chi s i ca thit (TMt) giO'a c i c nghipm thuc b i sung FOS. Tuy nhifin fi nghiem thuc F0S4 tfim cfi ehi s i gan tgy (4,79 ± 0,47%) cao han v i sai khic co y nghTa thong ke so vol c i c nghiem thuc cfin Igi v i tflm nufii bang thirc i n cfi cae ham lygng b i sung FOS khic.
Giai dogn Ifit xic cua tflm cfi anh huo'ng rat Ifl-n den cac chi tifiu sinh iy (Jussila, 1997a), Trong nghifin ciru niy, tflm dua v i o phin tich cfi cting giai dogn Iflt xie, vi the sy khic nhau ve cic chf s i niy phy thuflc vao FOS b i sung vio thirc i n , Chi s i sinh 1^ TSIw v i HSIw oja tflm su 6' F0S4 cao han o* DC, chirng tfl FOS da l i m tang siic khfle cDa tfim su nufii. Diiu niy CO Bie do FOS da cii thifin tic dfl tich luy ning lugng trong gan tyy v i ca thjt ciia tfim nufli (Fotedar v i cs, 1999). Kit qui tuang ty v i vai trfl cua prebiotic len cic chi s i sinh \</ ciia giip xac cung dugc quan s i t fi MOS tren marron (Sang v i Fotedar, 2010a), tflm hOm bflng (Sang v i Fotedar, 2010b).
cd i nghTa thing k6 (p < 0,05) IV. K^T LUAN VA K l i N NGHj 1.Kitlu$n
- Vific b i sung FOS vao thuc an khflng cii thifin ty lfi sing v i t i c dfi tang trufl'ng cua tfim sii nufli.
- Vfli ham lugng b i sung 0,2 - 0,4% FOS cii thifin mflt so chi tieu sinh 1^ cua tflm sO nufli, 2. K i i n nghj
- c i n cfl nhCrng nghien cu-u t i i p theo de lim rfi c i c ca c h i t i c dung cua FOS Ifin c i c chi>c nang sinh ly, tieu hoa, m i l n djch cOa tflm su, tgo ea sfl* khoa hpc d i i p dyng v i o thyc tien s i n x u i l .
- C i c yeu t i mien djch, sy thay d i i hfi vi smh vfit trong dufi'ng mfit v i khi nang khing Igi vi khuan cung nhu diiu kipn bit Igi ciia mfii tryfi'ng ciia tfim dugc cho an thuc an cfl bo sung FOS c i n dygc quan t i m nghifin ciru.
194 • TRUdNG OAI HQC NHA TRANG
p chi Khoa hoc - Cong nghe Thuy sdn
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