69 LAMPIRAN A SURAT DETERMINASI TANAMAN PUTRI MALU

(1)

69

LAMPIRAN A

(2)

70

LAMPIRAN B

(3)

71

LAMPIRAN C

(4)

72

LAMPIRAN D

DASAR PENGGUNAAN DOSIS

Dalam penelitian ini penggunaan dosis ditingkatkan berdasarkan faktor pengali 3,2. Untuk menentukan faktor pengali dapat diketahui dengan rumus sebagai berikut:

F = √ F = faktor pengali

n = jumlah dosis rentang -1 I =

I =

= 9,09 ~ 9,1 F = _√9,1 = x log 9,1

= x 0,96 = 0,48 ~ 0,5 = anti log 0,5 = 3,16 ~ 3,2

Dosis 1 = 550 mg/kg BB

Dosis 2 = 550 x 3,2 = 1760 mg/kg BB, (dosis yang dipakai 1750 mg/kg BB) Dosis 3 = 1750 x 3,2 = 5600 mg/kg BB, (dosis yang dipakai 5000 mg/kg

(5)

73

LAMPIRAN E

HASIL PERHITUNGAN KADAR ABU SIMPLISIA

No W (krus

kosong) (gram) W (bahan) (gram) W (krus + abu) (gram) % Kadar Abu

Rata – rata ± SD

1. 33,4309 2,0077 33,5226 4,57

4,44% ± 0,135 2. 33,9405 2,0053 33,0268 4,30

3. 32,6390 2,0087 32,7283 4,45

I. Kadar Abu = ( ) × 100% = , ,

, × 100% = 4,57% II. Kadar Abu = ( ) ×100%

= , ,

, × 100% = 4,30% III. Kadar Abu =( ) × 100%

= , ,

, × 100% = 4,45

(6)

74

LAMPIRAN F

HASIL PERHITUNGAN KADAR AIR SIMPLISIA

No. W (cawan

kosong)

(gram)

W (bahan)

(gram)

W (cawan +

simplisia)

(gram)

% Kadar

Air

Rata – rata± SD

1. 119,0803 2,0119 120,9250 8,31%

8,40%± 0,127 2. 85,2373 2,0049 87,0713 8,49%

I. Kadar Air = – × 100%

= , ,

, × 100% = 8,31%

II. Kadar Abu = – × 100%

= , _, , × 100% = 8,49%

(7)

75

LAMPIRAN G

HASIL PERHITUNGAN KADAR SARI LARUT AIR SIMPLISIA

No. W (Cawan kosong) (gram) W (bahan) (gram) W (cawan + filtrat) (gram)

% Kadar Sari Larut Etanol

Rata – rata± SD

1. 83,5523 5,0037 83,7050 15,26%

15,45% ± 0,18 2. 79,1089 5,0036 79,2652 15,62%

3. 57,3892 5,0052 57,5441 15,47%

1. Kadar Sari Larut Etanol = ( ) – × 100% = , ,

, × × 100% = 15,26% 2.Kadar Sari Larut Etanol = ( ) – ×100%

= , ,

, × × 100% = 15,62% 3.Kadar Sari Larut Etanol =( ) – × 100%

= , ,

, × × 100% = 15,47%

(8)

76

LAMPIRAN H

HASIL PERHITUNGAN KADAR SARI LARUT ETANOL SIMPLISIA

No. W (Cawan

kosong) (gram) W (bahan) (gram) W (cawan + filtrat) (gram)

% Kadar Sari

Larut Air

1. 83,5494 5,0028 83,6828 13,33%

13,60%± 0,38 2. 83,1287 5,0079 83,2676 13,87%

1.Kadar Sari Larut Air = ( ) – × 100% = , ,

, × × 100% = 13,33 % 2.Kadar Sari Larut Air = ( ) – ×100%

= , ,

, × × 100% = 13,87 %

(9)

(10)

78

LAMPIRAN I

SKRINING FITOKIMIA

(D) (E)

Keterangan :

A. Alkaloid dengan pereaksi dragendroff

B. Flavonoid dengan penambahan serbuk Mg, 2 ml alkohol khlorhidrik, dan amil alkohol

C. Saponin

D. Tanin dengan pereaksi FeCL3 dan larutan gelatin E. Terpenoid atau steroid

(11)

79

LAMPIRAN J

HASIL PERHITUNGAN KADAR ABU EKSTRAK

No W (krus

kosong) (gram) W (bahan) (gram) W (krus + abu) (gram) % Kadar Abu

1. 31,7720 2,0009 31,8652 4,64%

4,48% ± 0,226 2. 33,3549 2,0052 33,4415 4,32%

I. Kadar Abu = ( ) ×100% = , _, , × 100% = 4,64% II. Kadar Abu =( ) × 100%

= , _, , × 100% = 4,32%

(12)

80

LAMPIRAN K

HASIL PERHITUNGAN KADAR ABU TIDAK LARUT AIR

No W

(krus kosong) (gram) Bobot abu awal (gram) W (Krus + bahan) (gram) W (Krus + kertas saring) (gram) % Kadar Abu Larut Air

1. 33,3549 2,0052 33,4415 33,3677 3,68%

3,51% ±0,24 2. 31,7720 2,0009 31,8652 31,7983 3,34%

I. Kadar Abu = ( ) × 100% = , ,

, × 100% = 3,68% II. Kadar Abu = ( ) ×100%

= , ,

, × 100% = 3,34%

Rata – Rata = , , = 3,51%

(13)

81

LAMPIRAN L

HASIL PERHITUNGAN KADAR ABU TIDAK LARUT ASAM

No W

(krus kosong) (gram) Bobot abu awal (gram) W (Krus + bahan) (gram) W (Krus + kertas saring) (gram) % Kadar Abu Larut Air

1. 32,1937 2,0097 32,2276 32,2013 1,31%

1,43% ±0,170 2. 31,7524 2,0087 31,7941 31,7629 1,55%

I. Kadar Abu = ( ) ×100% = , ,

, × 100% = 1,31% II. Kadar Abu =( ) × 100%

= , ,

, × 100% = 1,55%

(14)

82

LAMPIRAN M

HASIL PERHITUNGAN KADAR AIR EKSTRAK

No. W (cawan

kosong)

(gram)

W (Zat)

(gram)

W (cawan +

simplisia)

(gram)

% Kadar

Air

1. 83,5648 5,0054 88,1658 8,01%

8,54%± 0,490 2. 88,1440 5,0012 92,7140 8,62%

3. 79,0626 5,0051 83,6179 8,98%

I. Kadar Air = × 100%

= , ,

, × 100% = 8,01%

II. Kadar Abu = ×100%

= , _, , × 100% = 8,62%

III. Kadar Abu × 100%

= , ,

, × 100% = 8,98%

(15)

83

LAMPIRAN N

PERHITUNGAN PENIMBANGAN EKSTRAK

Berat Badan Sebelum Perlakuan (gram) Kelompok

Kontrol

Kelompok Perlakuan 1 (Dosis 550 mg/kgBB)

elompok Perlakuan 1 (Dosis 1750

mg/kgBB)

Kelompok Perlakuan 1 (Dosis 5000 mg/kgBB)

26 21 33 28

29 29 28 27

28 26 29 36

17 32 29 33

Berikut adalah perhitungan penimbangan ekstrak untuk mencit jantan galur Swiss :

Vp = ( ) ( / ) , Keterangan :

Vp : Faktor Pengenceran D : Dosis

C : Konsentrasi,

berdasarkan rumus diatas maka dapat dihitung :

Dosis 550 mg/kgBB :

Vp(2,5ml)= × 550 : C = 59,4 mg = 0,0594 gram C = 0,0594 gram

2 ml = 0,0297 gram/ ml X 5 ml = 0,1485 gram/ 5ml

Dosis 1750 mg/kgBB :

Vp(2,5ml)= × 1750 : C = 208, 25mg = 0,20825 gram C = 0,20825 gram

2 ml = 0,1041 gram/ ml X 5 ml = 0,5206 gram/ 5ml

Dosis 5000 mg/kgBB :

Vp(2,5ml)= × 5000 : C = 620 mg = 0,62 gram C = 0,62 gram

(16)

84

LAMPIRAN O

PENIMBANGAN ORGAN (gram)

Kode BB

awal

BB akhir Bobot Organ (gram)

Jantung Ginjal Paru Testis Limfa Hati Vas. Differens

Kontrol 1 16 28 0,16 0,48 0,34 0,28 0,42 2,16 0,22

Kontrol 2 17 31 0,14 0,43 0,23 0,23 0,19 1,48 0,22

Kontrol 3 17 31 0,16 0,61 0,29 0,31 0,26 1,99 0,26

Kontrol 4 15 21 0,14 0,23 0,27 0,28 0,24 1,82 0,26

Dosis 550 mg/kgBB 16 25 0,09 0,34 0,19 0,24 0,25 1,31 0,15

Dosis 550 mg/kgBB 17 31 0,14 0,45 0,25 0,28 0,31 2,07 0,38

Dosis 550 mg/kgBB 16 32 0,16 0,33 0,35 0,34 0,13 1,51 0,27

Dosis 550 mg/kgBB 19 34 0,16 0,48 0,24 0,24 0,15 1,44 0,18

Dosis 1750 mg/kgBB 20 35 0,15 0,48 0,22 0,34 0,24 2,05 0,37

(17)

85

Dosis 1750 mg/kgBB 18 33 0,13 0,51 0,25 0,25 0,48 1,75 0,21

Dosis 1750 mg/kgBB 18 32 0,11 0,38 0,31 0,23 0,13 1,39 0,27

Dosis 5000 mg/kgBB 17 29 0,16 0,49 0,26 0,29 0,22 1,84 0,36

Dosis 5000 mg/kgBB 17 29 0,12 0,44 0,21 0,23 0,22 1,75 0,19

Dosis 5000 mg/kgBB 20 34 0,14 0,46 0,32 0,23 0,26 1,76 0,31

(18)

86

LAMPIRAN P

PERHITUNGAN INDEKS ORGAN (%)

Jantung Ginjal Paru Testis Limpa Hati Vas Differens

ontrol 1 0,16

28 × 100 = 0,57 0,48 28 × 100 = 1,71 0,34 28 × 100 = 1,21

0,28 28 × 100 = 1,00

0,42 28 × 100 = 1,50 2,16 28 × 100 = 7,71 0,22 28 × 100 = 0,79

ontrol 2 45 39 74 74 61 77 71

ontrol 3 52 97 94 00 84 42 84

ontrol 4 67 95 29 33 14 67 24

osis 550 mg/kgBB 0,36 1,36 0,76 0,96 1,00 5,24 0,60

Dosis 550 mg/kgBB 0,45 1,45 0,81 0,90 1,00 6,67 1,22

Dosis 550 mg/kgBB 0,50 1,22 1,30 1,26 0,48 5,59 1,00

Dosis 550 mg/kgBB 0,47 1,41 0,71 0,70 0,44 4,24 0,53

osis 1750 mg/kgBB 0,43 1,37 0,63 0,97 0,69 5,86 1,06

Dosis 1750 mg/kgBB 0,47 1,67 0,80 0,87 0,80 3,13 0,53

(19)

87

Dosis 1750 mg/kgBB 0,34 1,19 0,97 0,72 0,41 4,34 0,84

osis 5000 mg/kgBB 55 69 90 00 76 34 24

Dosis 5000 mg/kgBB 41 52 72 79 76 03 66

Dosis 5000 mg/kgBB 41 35 94 68 76 18 91

(20)

88

LAMPIRAN Q

HASIL PENGAMATAN KELOMPOK KONTROL

Keterangan :

(21)

(22)

90

LAMPIRAN R

(23)

(24)

92

LAMPIRAN S

(25)

(26)

94

LAMPIRAN T

(27)

(28)

96

LAMPIRAN U

ORGAN KELOMPOK KONTROL DAN KELOMPOK PERLAKUAN

Kontrol 1

(29)

97

Kontrol 3

(30)

98

Dosis 550 mg/kgBB (5)

(31)

99

(32)

100

(33)

101

(34)

102

(35)

103

(36)

104

LAMPIRAN V

LAMBUNG KELOMPOK KONTROL DAN KELOMPOK

PERLAKUAN

Kontrol

Perlakuan Dosis 550 mg/kgBB

Perlakuan Dosis 1750 mg/kgBB

(37)

102

LAMPIRAN W

PERHITUNGAN JUMLAH JENGUKAN DENGAN ONE WAY ANOVA

Test of Homogeneity of Variances

Jumlah_Jengukan

Levene Statistic df1 df2 Sig.

1.556 3 12 .251

ANOVA

Jumlah_Jengukan

Sum of

Squares Df Mean Square F Sig. Between Groups 8.188 3 2.729 .652 .597 Within Groups 50.250 12 4.188

Total 58.438 15

Test of Homogeneity of Variances

Jumlah_jengukan

7.643 3 12 .004

Test of Homogeneity of Variances ANOVA

Jumlah_jengukan

Sum of

Squares Df Mean Square F Sig. Between Groups 10.188 3 3.396 1.079 .395 Within Groups 37.750 12 3.146

(38)

103 Jumlah_Jengukan

.770 3 12 .533

Test of Homogeneity of Variances

Jumlah_Jengukan

.404 3 12 .753

ANOVA

Jumlah_Jengukan

Sum of Squares Df

Mean

Square F Sig. Between Groups 15.500 3 5.167 2.638 .097 Within Groups 23.500 12 1.958

Total 39.000 15

ANOVA

Jumlah_Jengukan

Sum of Squares Df

Mean

Square F Sig. Between Groups 29.500 3 9.833 10.26

1

.001

Within Groups 11.500 12 .958

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104

Post Hoc Tests

Multiple Comparisons

Jumlah_Jengukan Tukey HSD

(I) Parameter (J) Parameter

Mean

Difference (I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

Kontrol Dosis 550 mg/kgBB -.750 .692 .706 -2.81 1.31

Dosis 1750 mg/kgBB -2.500* .692 .016 -4.56 -.44

Dosis 5000 mg/kgBB 1.250 .692 .317 -.81 3.31

Dosis 550 mg/kgBB

Kontrol .750 .692 .706 -1.31 2.81

Dosis 1750 mg/kgBB -1.750 .692 .105 -3.81 .31

Dosis 5000 mg/kgBB 2.000 .692 .057 -.06 4.06

Dosis 1750 mg/kgBB

Kontrol 2.500* _.692 _.016 _.44 _4.56

Dosis 550 mg/kgBB 1.750 .692 .105 -.31 3.81

Dosis 5000 mg/kgBB 3.750* .692 .001 1.69 5.81

Dosis 5000 mg/kgBB

Kontrol -1.250 .692 .317 -3.31 .81

Dosis 550 mg/kgBB -2.000 .692 .057 -4.06 .06

Dosis 1750 mg/kgBB -3.750* _.692 _.001 _-5.81 _-1.69

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105

Homogeneous Subsets

Jumlah_Jengukan

Tukey HSDa

Parameter N

Subset for alpha = 0.05

1 2

Dosis 5000 mg/kgBB 4 1.50

Kontrol 4 2.75

Dosis 550 mg/kgBB 4 3.50 3.50

Dosis 1750 mg/kgBB 4 5.25

Sig. .057 .105

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 4,000.

Test of Homogeneity of Variances

Jumlah_Jengukan

1.493 3 12 .266

ANOVA

Jumlah_Jengukan

Sum of

Squares Df Mean Square F Sig. Between Groups 23.500 3 7.833 2.575 .103 Within Groups 36.500 12 3.042

(41)

106

Test of Homogeneity of Variances

Jumlah_Jengukan

1.338 3 12 .308

ANOVA

Jumlah_Jengukan

Sum of Squares df

Mean

Total 89.438 15

Test of Homogeneity of Variances

Jumlah_Jengukan

2.267 3 12 .133

ANOVA

Jumlah_Jengukan

Sum of

Squares Df

Mean

Square F Sig. Between Groups 13.688 3 4.563 .753 .542 Within Groups 72.750 12 6.063

(42)

107

Test of Homogeneity of Variances

Jumlah_Jengukan

1.811 3 12 .199

ANOVA

Jumlah_Jengukan

Sum of

Squares Df

Mean

(43)

108

LAMPIRAN X

PERHITUNGAN WAKTU MENGGELANTUNG DENGAN ONE WAY

ANOVA

Test of Homogeneity of Variances

Waktu_Menggelantung

5.697 3 12 .012

ANOVA

Waktu_Menggelantung Sum of Squares df

Mean

Square F Sig. Between

Groups

72.610 3 24.203 1.126 .378

Within Groups 258.031 12 21.503 Total 330.640 15

Test of Homogeneity of Variances

Waktu_Menggelantung

34.762 3 12 .000

ANOVA

Waktu_Menggelantung Sum of

Squares df Mean Square F Sig. Between Groups 241.470 3 80.490 1.544 .254 Within Groups 625.710 12 52.142

(44)

109

Test of Homogeneity of Variances

Waktu_menggelantung

.492 3 12 .694

ANOVA

Waktu_menggelantung Sum of

Squares Df

Mean

Total 902.613 15

Test of Homogeneity of Variances

Waktu_Menggelantung

.617 3 12 .617

ANOVA

Squares Df

Mean

(45)

110

Test of Homogeneity of Variances

Waktu_Menggelantung

6.000 3 12 .010

ANOVA

Mean

Square F Sig. Between

Groups

30.583 3 10.194 .667 .588

Within Groups 183.493 12 15.291 Total 214.076 15

Test of Homogeneity of Variances

Waktu_Menggelantung

3.882 3 12 .038

ANOVA

Mean

Total 593.364 15

Test of Homogeneity of Variances

Waktu_Menggelantung

(46)

111

ANOVA

Waktu_Menggelantung

Sum of

Squares Df Mean Square F Sig.

Between Groups 60.182 3 20.061 .416 .744 Within Groups 578.158 12 48.180

Total 638.339 15

Test of Homogeneity of Variances

Waktu_Menggelantung

.711 3 12 .564

ANOVA

Squares Df Mean Square F Sig. Between

Groups

208.195 3 69.398 .759 .538

Within Groups 1097.507 12 91.459

(47)

112

LAMPIRAN Y

PERHITUNGAN INDEKS ORGAN DENGAN ONE WAY ANOVA

Test of Homogeneity of Variances

Organ_Ginjal

.573 3 12 .644

ANOVA

Organ_Ginjal

Sum of

Squares Df

Mean

Square F Sig. Between Groups .408 3 .136 3.109 .067 Within Groups .525 12 .044

Total .933 15

Test of Homogeneity of Variances

Organ_Hati

.432 3 12 .734

ANOVA

Organ_Hati

Sum of

Squares Df

Mean

(48)

113

Test of Homogeneity of Variances

Organ_Jantung

.321 3 12 .810

ANOVA

Organ_Jantung

Sum of Squares df

Mean

Total .108 15

Test of Homogeneity of Variances

Organ_Limpa

1.367 3 12 .300

ANOVA

Organ_Limpa

Sum of Squares df

Mean

Square F Sig. Between Groups .281 3 .094 .792 .521 Within Groups 1.421 12 .118

Total 1.702 15

Test of Homogeneity of Variances

Organ_Paru

(49)

114

ANOVA

Organ_Paru

Sum of

Squares df

Mean

Total .541 15

Test of Homogeneity of Variances

Organ_Testis

.330 3 12 .804

ANOVA

Organ_Testis

Sum of

Squares df

Mean

Total .422 15

Test of Homogeneity of Variances

Organ_vasDifferen

(50)

115

ANOVA

Organ_vasDifferen

Sum of

Squares df

Mean

Square F Sig. Between Groups .094 3 .031 .473 .707 Within Groups .797 12 .066