Lampiran 3
Gambar Karakterisasi Tumbuhann Nimba
Lampiran 3
(lanjutan)
Daun nimba segar
Lampiran4
Perhitungan Pemeriksaan Karakterisasi Serbuk Simplisia Daun Nimba
(Azadirachta indica
A. Juss.)
1.
Penetapan kadar air
a.
Berat sampel
= 5,002 g
Lampiran 4
(Lanjutan)
a.
Perhitungan hasil penetapan kadar sari yang larut dalam air
Lampiran 4
(Lanjutan)
c. Perhitungan hasil penetapan kadar sari yang larut dalam etanol
1.
Kadar sari larut dalam etanol I
Berat Cawan
= 44,246 g
Berat Cawan + Berat Sari
= 44,351 g
Berat Sampel
= 5,020 g
Berat sari
= 0,105 g
Kadar sari larut dalam etanol
,,
x
x
100%=10,45%
3.
Kadar sari larut dalam etanol III
Berat Cawan
= 45,129 g
Berat Cawan + Berat Sari
= 45,221 g
Berat Sampel
= 5,002 g
Berat sari
= 0,092 g
Kadar sari larut dalam etanol
,,
x
x
100% =9,19%
Kadar sari larut dalam etanol rata-rata
, % , % , %= 9,58%
Lampiran 4
(Lanjutan)
d. Perhitungan hasil penetapan kadar abu total
Lampiran 4
(lanjutan)
e. Perhitungan Hasil Penetapan Kadar Abu Tidak Larut Dalam Asam
1. Sampel I
Berat simplisia
= 2,002 g
Berat abu
= 0,009g
Kadar abu tidak larut asam
,,
x
100%
= 0,44%
2. Sampel II
Berat simplisia
= 2,001 g
Berat abu
= 0,001g
Kadar abu tidak larut asam
,,
100%
= 0,49%
3.Sampel III
Berat simplisia
= 2,005 g
Berat abu
= 0,007g
Kadar abu tidak larut asam
,,
x
100%
= 0,34%
Kadar abu tidak larut asam rata-rata
, % . % , %=
0,43%
Lampiran 5
Bagan Kerja Pembuatan Serbuk Simplisia
dibersihkan dari pengotor
dicuci hingga bersih
ditiriskan
ditimbang
dikeringkan di lemari
pengering
Simplisia kering 1,1kg
Serbuk simplisia daun nimba
Lampiran 6
Bagan Kerja Pembuatan Ekstrak
dibasahi dengan etanol 70%
dimasukkan ke dalam bejana tertutup
sekurang kurangnya selama 3 jam
dimasukkan ke dalam perkolator
ditambahkan cairan penyari sampai
satu lapis diatas serbuk
ditutup dengan alumunium foil
didiamkan selama 24 jam
dibuka kran perkolator dengan
kecepatan pengaliran 1 ml/menit.
dipekatkan dengan alat
rotary
evaporator
pada suhu 60°C
dimasukkan ke dalam alat
freeze
dryer
Serbuk Simplisia 300 g
Simplisia basah dalam perkolator
Simplisia dan pelarut berwarna hijau tua
Ekstrak etanol encer 10 L
Ekstrak etanol kental
Lampiran 7
Gambar alat-alat
Hemositometer
(Improved Neubauer-Marienfield)
Pipet Thoma leukosit
Lampiran 7
(Lanjutan)
Mikroskop
(Boeco-Germany)
Lampiran 8
(Lanjutan)
Neutrofil
Monosit
Lampiran 8
(Lanjutan)
Eusinofil
Lampiran 8
(Lanjutan)
Basofil
Lampiran 9
Contoh Perhitungan
Contoh perhitungan ekstrak yang akan ditimbang
Ekstrak yang akan ditimbang bervariasi konsentrasi yaitu 0,25%, 0,5% dan
1%
a.
Cara perhitungan ekstrak : misalnya BB mencit = 25g
0,25%=
,=
= 2,5mg/ml
Jumlah ekstrak etanol daun nimbi dosis 50mg/kg BB =
x 50 mg= 1,25
mg
Jumlah suspensi yang akan diberikan =
,, /
= 0,5 ml
0,5%=
,=
= 5 mg/ml
Jumlah ekstrak etanol daun nimbi dosis 100 mg/kg BB =
x 100 mg = 2,5
mg
Jumlah suspensi yang akan diberikan =
,/
= 0,5 ml
1%=
=
= 10 mg/ml
Jumlah ekstrak etanol daun nimbi dosis 200 mg/kg BB =
x 200 mg = 5
mg
Jumlah suspensi yang akan diberikan =
/
= 0,5 ml
b.
Contoh perhitungan jumlah total leukosit mencit
Jumlah leukosit =
x 20 x 10 atau (A+B+C+D) x 50 sel/
l
c.
Contoh perhitungan diferensial leukosit mencit
Jumlah diferensial mencit =
/Lampiran 10
Hasil Analisis Data Anava
Two Way
Total leukosit
Descriptive Statistics
Dependent Variable: hasil pengamatan leukosit
Waktu pengambilan
darah leukosit
Jenis perlakuan pengambilan
darah leukosit Mean Std. Deviation N
jam ke-6 Kontrol 8725.00 5700.959 5
Ovalbumin 8725.00 5700.959 5
dosis 50 mg/kg bb 6865.00 2893.074 5
dosis 100 mg/kg bb 4370.00 1103.744 5
dosis 200 mg/kg bb 6865.00 2893.074 5
Total 7110.00 4062.327 25
jam ke-24 Kontrol 5800.00 1575.496 5
Ovalbumin 9250.00 4663.723 5
dosis 50mg/kg bb 7695.00 3240.968 5
dosis 100mg/kg bb 8020.00 1025.061 5
dosis 200 mg/kg bb 9340.00 711.864 5
Total 8021.00 2788.534 25
hari ke-7 Kontrol 9250.00 4663.723 5
Ovalbumin 18330.00 5385.089 5
dosis 50mg/kg bb 15200.00 1303.572 5
dosis 100 mg/kg bb 9582.00 2178.000 5
dosis 200mg/kg bb 11366.00 1057.162 5
Total 12745.60 4743.165 25
Total Kontrol 7925.00 4321.789 15
Ovalbumin 12101.67 6679.563 15
dosis 50mg/kg bb 9920.00 4575.575 15
dosis 100 mg/kg bb 7324.00 2667.211 15
dosis 200mg/kg bb 9190.33 2546.600 15
Hasil pengamatan leukosit
TukeyHSDa,,b dosis 100mg/kg bb 15 7324.00
kontrol 15 7925.00
dosis 200mg/kg bb 15 9190.33 9190.33
dosis 50mg/kg bb 15 9920.00 9920.00
ovalbumin 15 12101.67
Sig. .248 .154
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 11837588,333.
a. Uses Harmonic Mean Sample Size = 15,000.
b. Alpha = ,05.
Tests of Between-Subjects Effects
Dependent Variable: hasil pengamatan leukosit
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 8.700E8 14 6.214E7 5.249 .000
Intercept 6.476E9 1 6.476E9 547.060 .000
waktu 4.576E8 2 2.288E8 19.328 .000
perlakuan 2.106E8 4 5.265E7 4.448 .003
waktu * perlakuan 2.018E8 8 2.522E7 2.130 .046
Error 7.103E8 60 1.184E7
Total 8.056E9 75
Corrected Total 1.580E9 74
Limfosit
Descriptive Statistics
Dependent Variable: hasil pengamatan limfosit
Waktu pengambilan
darah limfosit
Jenis perlakuan pengambilan
darah limfosit Mean
Test of Between-subjects Effects
Dependent Variable: hasil pengamatan limfosit
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 11764.347a 14 840.310 46.962 .000
Intercept 86836.053 1 86836.053 4852.984 .000
waktu 3927.707 2 1963.853 109.753 .000
pengamatan 5023.547 4 1255.887 70.187 .000
waktu * pengamatan 2813.093 8 351.637 19.652 .000
Error 1073.600 60 17.893
Total 99674.000 75
Corrected Total 12837.947 74
a. R Squared = ,916 (Adjusted R Squared = ,897)
Hasil pengamatan limfosit
pengamatan N
Subset
1 2
TukeyHSDa,,b dosis 200 mg/kg bb 15 28.53
kontrol 15 29.20
dosis 100mg/kg bb 15 29.93
dosis 50mg/kg bb 15 32.27
ovalbumin 15 50.20
Sig. .125 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 17,893.
a. Uses Harmonic Mean Sample Size = 15,000.
Monosit
Descriptive Statistics
Dependent Variable: hasil pengamatan monosit
Waktu pengambilan
darah monosit
Jenis perlakuan pengambilan
darah monosit Mean
Std.
ovalbumin 12.00 1.581 5
dosis 50mg/kg bb 8.00 2.000 5
dosis 100mg/kg bb 8.60 2.074 5
dosis 200mg/kg bb 6.60 2.074 5
Total 8.24 2.743 25
Total kontrol 6.00 1.558 15
ovalbumin 9.33 3.132 15
dosis 50mg/kg bb 7.60 1.882 15
dosis 100mg/kg bb 6.20 2.541 15
dosis 200mg/kg bb 6.27 1.751 15
Tests of Between-Subjects Effects
Dependent Variable: hasil pengamatan monosit
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 249.520a 14 17.823 4.774 .000
Intercept 3759.480 1 3759.480 1007.004 .000
waktu 61.040 2 30.520 8.175 .001
perlakuan 119.253 4 29.813 7.986 .000
waktu * perlakuan 69.227 8 8.653 2.318 .031
Error 224.000 60 3.733
Total 4233.000 75
Corrected Total 473.520 74
a. R Squared = ,527 (Adjusted R Squared = ,417)
Hasil pengamatan monosit
Jenis perlakuan
pengambilan darah
monosit N
Subset
1 2
TukeyHSDa,,b kontrol 15 6.00
dosis 100 mg/kg bb 15 6.20
dosis 200mg/kg bb 15 6.27
dosis 50mg/kg bb 15 7.60 7.60
ovalbumin 15 9.33
Sig. .170 .115
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 3,733.
a. Uses Harmonic Mean Sample Size = 15,000.
Neutrofil
Descriptive Statistics
Dependent Variable: hasil pengamatan neutrofil
Jenis perlakuan pengambilan
darah neutrofil
Waktu pengambilan
darah neutrofil Mean Std. Deviation N
Tests of Between-Subjects Effects
Dependent Variable: hasil pengamatan neutrofil
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 9950.880a 14 710.777 14.997 .000
Intercept 331070.520 1 331070.520 6985.593 .000
perlakuan 6411.013 4 1602.753 33.818 .000
waktu 2744.240 2 1372.120 28.952 .000
perlakuan * waktu 795.627 8 99.453 2.098 .050
Error 2843.600 60 47.393
Total 343865.000 75
Corrected Total 12794.480 74
a. R Squared = ,778 (Adjusted R Squared = ,726)
Hasil pengamatan neutrofil
Jenis perlakuan
pengambilan darah
neutrofil N
Subset
1 2
TukeyHSDa,,b dosis 200 mg/kg bb 15 60.07
dosis 100 mg/kg bb 15 61.07
Kontrol 15 61.67
dosis 50mg/kg bb 15 64.73
ovalbumin 15 84.67
Sig. .352 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 47,393.
a. Uses Harmonic Mean Sample Size = 15,000.
Eusinofil
Descriptive Statistics
Dependent Variable: hasil pengamatan eusinofil
Jenis perlakuan pengambilan
darah basofil
Waktu pengambilan darah
Tests of Between-Subjects Effects
Dependent Variable: hasil pengamatan eusinofil
Source
Type III Sum of
Squares Df Mean Square F Sig.
Corrected Model 251.467a 14 17.962 19.524 .000
Intercept 1160.333 1 1160.333 1261.232 .000
perlakuan 209.733 4 52.433 56.993 .000
waktu 6.907 2 3.453 3.754 .029
perlakuan * waktu 34.827 8 4.353 4.732 .000
Error 55.200 60 .920
Total 1467.000 75
Corrected Total 306.667 74
a. R Squared = ,820 (Adjusted R Squared = ,778)
Hasil pengamatan eusinofil
Jenis perlakuan
pengambilan darah
basofil N
Subset
1 2 3
TukeyHSDa,,b kontrol 15 2.53
dosis 100mg/kg bb 15 2.87
dosis 200mg/kg bb 15 3.13 3.13
dosis 50mg/kg bb 15 4.00
ovalbumin 15 7.13
Sig. .434 .110 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = ,920.
a. Uses Harmonic Mean Sample Size = 15,000.
Basofil
Descriptive Statistics
Dependent Variable: hasil pengamatan basofil
Jenis perlakuan
pengambilan darah basofil
Tests of Between-Subjects Effects
Dependent Variable: hasil pengamatan basofil
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 162.347a 14 11.596 20.957 .000
Intercept 141.453 1 141.453 255.639 .000
perlakuan 131.280 4 32.820 59.313 .000
waktu 5.787 2 2.893 5.229 .008
perlakuan * waktu 25.280 8 3.160 5.711 .000
Error 33.200 60 .553
Total 337.000 75
Corrected Total 195.547 74
a. R Squared = ,830 (Adjusted R Squared = ,791)
Hasil pengamatan basofil
Jenis perlakuan
pengambilan darah
basofil N
Subset
1 2
TukeyHSDa,,b dosis 100mg/kg bb 15 .53
Kontrol 15 .60
dosis 200 mg/kg bb 15 .73
dosis 50 mg/kg bb 15 1.00
ovalbumin 15 4.00
Sig. .431 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = ,553.
a. Uses Harmonic Mean Sample Size = 15,000.
