40
Lampiran 2.
Rekomendasi persetujuan etik penelitian kesehatan
42
Lampiran 3 (lanjutan)
Lampiran 4. Gambar mikroskopik serbuk simplisia daun pegagan (
Centella
asiatica
(L.) Urb.) pada perbesaran 10 x 10
Keterangan : 1 = Epidermis dengan stomata
2 = Rambut penutup
3 = Serabut sklerenkim
44
Lampiran 5. Bagan alur penelitian
Daun pegagan
Simplisia
Serbuk
Karakterisasi simplisia
Skrining fitokimia
-
Pemeriksaan makroskopik
-
Pemeriksaan mikroskopik
-
Penetapan kadar air
-
Penetapan kadar sari larut
dalam air
-
Penetapan kadar sari larut
dalam etanol
-
Penetapan kadar abu total
-
Penetapan kadar abu tidak
larut dalam asam
Pemeriksaan :
-
Alkaloid
-
Flavonoida
-
Saponin
-
Tanin
-
Steroid/triterpenoid
Dicuci dari pengotor hingga bersih
Ditiriskan
Ditimbang
Dikeringkan di lemari pengering
Dihaluskan
Lampiran 5 (lanjutan)
Diremaserasi dengan
etanol 70 % hingga
diperoleh 100 bagian
maserat
Serbuk simplisia
(300 gram)
Ampas
Maserat
1
Dimaserasi dengan 75 bagian
etanol 70 %
Ekstrak etanol kental
148.58 gram
Diuapkan menggunakan
rotary evaporator
Depekatkan di atas penangas air
Maserat
2
Maserat
Karakterisasi
Penetapan:
-
Kadar air
-
Kadar sari larut dalam
air
-
Kadar sari larut dalam
etanol
-
Kadar abu total
-
Kadar abu tidak larut
dalam asam
Pengujian aktivitas
antihiperurisemia
Pengukuran
kadar asam urat
Skrining fitokimia
Pemeriksaan :
-
Alkaloida
-
Flavonoida
-
Saponin
-
Tanin
46
Lampiran 6. Perhitungan kadar air serbuk simplisia daun pegagan
% Kadar Air =
x 100%
1.
% Kadar Air =
x100% = 4,0 %
2.
% Kadar Air =
x100% = 3,9 %
3.
% Kadar Air =
x100% = 4,0 %
% Kadar Air Rata-Rata =
= 3,97 %
No
Berat Sampel (g)
Volume Air (ml)
1.
2.
3.
5,0
5,1
5,0
0,2
0,2
0,2
Lampiran 7. Perhitungan penetapan kadar sari larut dalam air serbuk simplisia
daun pegagan
1.
% Kadar Sari larut air =
x
x100% = 17,28%
2.
% Kadar Sari larut air =
x
x100% = 17,18%
3.
% Kadar Sari larut air =
x
x100% = 17,28%
% Kadar Sari Larut air rata-rata =
= 17,24 %
No
Berat Sampel (g)
Berat Sari (g)
1.
2.
3.
5,005
5,006
5,005
0,173
0,172
0,173
% Kadar sari larut air =
Berat sari (g)
Berat sampel (g)
100
48
Lampiran 8. Perhitungan penetapan kadar sari larut dalam etanol serbuk simplisia
daun pegagan
No.
Berat sampel (g)
Berat sari (g)
Kadar (%)
1.
5,0
0,16
16
2.
5,0
0,15
15
3.
5,0
0,15
15
1.
% Kadar sari larut dalam etanol =
0,16 g
5 g
100
20
100% = 16 %
2.
% Kadar sari larut dalam etanol =
0,15 g
5 g
100
20
100% = 15 %
3.
% Kadar sari larut dalam etanol =
0,15 g
5 g
100
20
100% = 15 %
% Kadar rata-rata =
16 15 15
3
=
15,33%
% Kadar sari larut etanol =
Berat sari (g)
Berat sampel (g)
100
20
100%
Lampiran 9. Perhitungan penetapan kadar abu total serbuk simplisia daun pegagan
1.
% Kadar abu total =
0,1574
2,135
g
100% =
7,37%
2.
% Kadar abu total =
0,1714
2,0156 g
100% = 8,50%
3.
% Kadar abu total =
0,1593
2,0524 g
100% = 7,76%
% Kadar rata-rata =
7,73
8,50
7,76
3
100% = 7,87%
No. Berat sampel (g)
Berat sari (g)
Kadar (%)
1.
2,1353
0,1574
7,37
2.
2,0156
0,1714
8,50
3.
2,0524
0,1593
7,78
% Kadar abu total =
Berat abu total (g)
50
Lampiran 10. Perhitungan penetapan kadar abu tidak larut dalam asam simplisia
daun pegagan
No.
Berat sampel (g)
Berat abu (g)
Kadar (%)
1.
2,0012
0,1002
5,0
2.
2,0002
0,1020
5,1
3.
2,0001
0,1000
5,0
1.
% Kadar abu tidak larut dalam asam =
0,1002
2,0012 g
100% = 5,0%
2.
% Kadar abu tidak larut dalam asam =
0,10
0
2,0002 g
100% = 5,1%
3.
% Kadar abu tidak larut dalam asam =
0,1000
2,0001 g
100% = 5,0%
% Kadar rata-rata=
5,0
5,1
5,0
3
= 5,03%
% Kadar abu tidak larut dalam asam
=
Berat sampel (g)
Berat abu (g)
x
100%
Lampiran 11. Volume maksimum sesuai jalur pemberian dan konversi dosis.
1.
Tabel volume maksimum larutan sediaan uji yang dapat diberikan pada hewan
uji (Ritschel, 1974)
Jenis hewan uji
Volume maksimal (ml) sesuai jalur
pemberian
i.v
i.m
i.p
s.c
p.o
Mencit (20-30 g)
Tikus (100 g)
Hamster (50 g)
Marmut (250 g)
Merpati (300 g)
Kelinci (2,5 kg)
Kucing (3 kg)
Anjing (5 kg)
0,5
0,1
-
-
2
5-10,
5-10
10-20
0,05
0,1
0,1
0,25
0,5
0,5
1
5
1
2-5
1-2
2-5
2
10-20
10-20
20-30
0,5-1
2-5
2,5
5
2
5-10
5-10
10
1
5,0
2,5
10
10
20
50
100
2.
Tabel konversi dosis antara jenis hewan dengan manusia (Laurence, dkk., 2008)
Mencit
20 g
Tikus
200 g
Marmut
400 g
Kelinci
1,2 kg
Kera
4 kg
Anjing
12 kg
Manusia
70 kg
Mencit
20g
1,0
7,0
12,25
27,8
64,1
124,2
387,9
Tikus
200g
0,14
1,0
1,74
3,9
9,2
17,8
56,0
Marmut
400 g
0,08
0,57
1,0
2,25
5,2
10,2
31,5
Kelinci
1,2 kg
0,04
0,25
0,44
1,0
2,4
4,5
14,2
Kera
4 kg
0,016
0,11
0,19
0,42
1,0
1,9
6,1
Anjing
12 kg
0,008
0,06
0,10
0,22
0,52
1,0
3,1
Manusia
52
Lampiran 12. Perhitungan volume pemberian EEDP dosis 200 mg/kg BB, 400
mg/kg BB dan 600 mg/kg BB, allopurinol dosis 10 mg/kg BB dan
potassium
oxonat
dosis 200 mg/kg BB.
1.
EEDP dosis 200 mg/kg BB
Berat badan mencit = 20 g
Dosis pemberian
=
= 4 mg
Konsentrasi ekstrak = 20 mg/ ml
Volume pemberian
=
x 1 ml = 0,2 ml.
2.
EEDP dosis 400 mg/kg BB
Berat badan mencit
= 20 g
Dosis pemberian
=
= 8 mg
Konsentrasi ekstrak = 40 mg/ml
Volume pemberian
=
x 1 ml = 0,2 ml.
3.
EEDP dosis 600 mg/kg BB
Berat badan mencit
= 20 g
Dosis pemberian
=
= 12 mg
Konsentrasi ekstrak = 60 mg/ml
Volume pemberian
=
x 1 ml = 0,2 ml.
4.
Dosis allopurinol yang digunakan 10 mg/kg BB, maka volume
pemberiannya adalah:
Dosis untuk 20 g mencit
=
x 20 g = 0,2 mg
Konsentrasi allopurinol
= 1 mg/ml
Volume pemberian
=
x 1 ml = 0,2 ml
5.
Dosis
potassium
oxonat
yang digunakan 200 mg/kg BB, maka volume
pemberiannya adalah:
Dosis untuk 20 g mencit
=
x 20 g = 4 mg
Konsentrasi lar.
potassium
oxonat
= 6 mg/ml
Volume pemberian
=
54
Lampiran 13. Gambar hewan percobaan
56
Lampiran 15. Data pengukuran kadar asam urat
Perlakuan
Perulangan
Berat
Badan
(g)
Kadar asam
urat puasa
( mg/dl )
Kadar asam
urat terinduksi
(mg/dl)
Kadar asam urat (mg/dl)
1 jam
2
jam
3 jam
4 jam
CMC-Na
(kontrol negatif)
1
29
1.7
3.0
3.0
3.6
4.0
4.3
2
26
1.6
3.2
3.3
3.4
3.4
3.8
3
25
1.6
4.1
4.2
4.2
5.3
5.8
4
28
1.7
4.0
4.2
4.5
5.0
6.1
5
26
1.5
3.6
3.7
4.0
5.1
4.1
Rata-rata
26.8
1.62
3.58
3.68
3.94
4.56
4.82
Suspensi
Allopurinol 10
mg/kg BB
(kontrol positif)
1
26
1.5
3.0
2.7
2.6
2.1
1.6
2
27
1.8
3.6
3.3
2.8
2.4
2.0
3
26
1.5
2.8
2.6
2.2
2.0
1.5
4
25
1.6
3.3
2.9
2.4
2.3
2.1
5
25
1.6
3.4
3.0
2.5
2.2
1.8
Rata-rata
25.8
1.6
3.2
2.9
2.5
2.2
1.8
Suspensi EEDP
200 mg/kg BB
1
25
1.7
4.2
4.1
3.8
3.5
2.8
2
26
1.6
2.8
2.6
2.4
2.2
2.1
3
25
1.6
3.0
3.0
2.7
2.5
2.0
4
26
1.7
4.0
3.8
3.6
3.2
2.7
5
26
1.5
3.2
3.1
3.0
2.8
2.6
Rata-rata
25.6
1.62
3.4
3.3
3.1
2.8
2.4
Suspensi EEDP
400 mg/kg BB
1
26
1.5
3.0
2.8
2.6
2.2
1.8
2
30
1.8
3.2
3.0
2.4
2.0
1.6
3
28
1.6
3.8
3.6
3.4
3.0
2.5
4
26
1.6
3.4
3.3
2.8
2.6
2.2
5
26
1.6
3.7
3.5
3.2
2.6
2.1
Rata-rata
27.2
1.62
3.4
3.2
2.9
2.5
2.0
Suspensi EEDP
600 mg/kg BB
1
25
1.6
4.1
3.7
3.4
3.1
2.3
2
26
1.5
3.8
3.5
3.2
2.6
2.1
3
25
1.6
3.1
2.9
2.5
2.1
1.5
4
24
1.6
3.3
3.2
2.7
2.1
2.1
5
25
1.6
2.9
2.9
2.4
2.2
1.8
Rata-rata
25
1.58
3.4
3.2
2.8
2.4
1.9
Lampiran 16. Data perhitungan persen pengukuran kadar asam urat
Perlakuan
Perulangan
Persen penurunan kadar asam urat (%)
1 jam
2 jam
3 jam
4 jam
CMC-Na
(kontrol
negatif)
1
.0
-20.00
-33.33
-43.33
2
-3.13
-6.25
-6.25
-18.75
3
-2.44
-2.44
-29.27
-41.46
4
-5.00
-12.50
-25.00
-52.50
5
-2.78
-11.11
-41.67
-13.89
Rata-rata
-2.67
-10.46
-27.10
-33.99
Suspensi
Allopurinol
10 mg/kg
BB
(kontrol
positif)
1
10.00
13.33
30.00
51.85
2
8.33
22.22
33.33
48.48
3
7.14
21.43
28.57
50.00
4
12.12
27.27
30.30
41.38
5
11.76
26.47
35.29
53.33
Rata-rata
9.87
22.15
31.50
49.01
Suspensi
EEDP 200
mg/kg BB
1
2.38
9.52
16.67
33.33
2
7.14
14.29
21.43
25.00
3
0.00
10.00
16.67
33.33
4
5.00
10.00
20.00
32.50
5
3.13
6.25
12.50
18.75
Rata-rata
3.53
10.01
17.45
28.58
Suspensi
EEDP 400
mg/kg BB
1
6.67
13.33
26.67
40.00
2
6.25
25.00
37.50
50.00
3
5.26
10.53
21.05
34.21
4
2.94
17.65
23.53
35.29
5
5.41
13.51
29.73
43.24
Rata-rata
5.31
16.00
27.70
40.55
Suspensi
EEDP 600
mg/kg BB
1
9.76
17.07
24.39
43.90
2
7.89
15.79
31.58
44.74
3
6.45
19.35
32.26
51.61
4
3.03
18.18
36.36
36.36
5
0.00
17.24
24.14
37.93
58
Lampiran 17. Hasil analisis statistik
Tests of Normality
perlakuan
Kolmogorov-Smirnova
Shapiro-Wilk
Statistic df Sig. Statistic df Sig. jam_ke1 CMC Na .249 5 .200*
.949 5 .729 Suspensi allopurinol 10 mg/kg
BB
.210 5 .200*
.927 5 .574
suspensi EEDP 200 mg/kg BB .159 5 .200* .993 5 .988 suspensi EEDP 400 mg/kg BB .287 5 .200* .883 5 .325 suspensi EEDP 600 mg/kg BB .203 5 .200*
.961 5 .812 jam_ke2 CMC Na .180 5 .200* .978 5 .923
Suspensi allopurinol 10 mg/kg BB
.249 5 .200* .892 5 .366
suspensi EEDP 200 mg/kg BB .302 5 .155 .912 5 .477 suspensi EEDP 400 mg/kg BB .271 5 .200* .895 5 .383 suspensi EEDP 600 mg/kg BB .185 5 .200* .983 5 .950 jam_ke3 CMC Na .237 5 .200* .940 5 .663
Suspensi allopurinol 10 mg/kg BB
.269 5 .200*
.926 5 .571
suspensi EEDP 200 mg/kg BB .211 5 .200*
.945 5 .701 suspensi EEDP 400 mg/kg BB .175 5 .200* .949 5 .729 suspensi EEDP 600 mg/kg BB .243 5 .200* .884 5 .330 jam_ke4 CMC Na .272 5 .200* .890 5 .356
Suspensi allopurinol 10 mg/kg BB
.255 5 .200*
.889 5 .354
suspensi EEDP 200 mg/kg BB .326 5 .088 .806 5 .090 suspensi EEDP 400 mg/kg BB .194 5 .200* .936 5 .635 suspensi EEDP 600 mg/kg BB .194 5 .200*
.939 5 .661 a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variances
Levene Statistic df1 df2 Sig. jam_ke1 2.137 4 20 .114 jam_ke2 1.730 4 20 .183 jam_ke3 2.121 4 20 .116 jam_ke4 7.053 4 20 .001
ANOVA
Sum of Squares df Mean Square F Sig. jam_ke1 Between Groups 412.392 4 103.098 15.874 .000
Within Groups 129.895 20 6.495
Total 542.287 24
jam_ke2 Between Groups 3266.575 4 816.644 34.932 .000 Within Groups 467.559 20 23.378
Total 3734.134 24
jam_ke3 Between Groups 12129.525 4 3032.381 57.795 .000 Within Groups 1049.365 20 52.468
Total 13178.890 24
Kruskal-Wallis Test
Ranks
perlakuan N Mean Rank jam_ke4 CMC Na 5 3.00
Suspensi allopurinol 10 mg/kg BB
5 21.30
suspensi EEDP 200 mg/kg BB 5 8.00 suspensi EEDP 400 mg/kg BB 5 15.30 suspensi EEDP 600 mg/kg BB 5 17.40
Total 25
Test Statisticsa,b
jam_ke4 Chi-Square 20.188
df 4
Asymp. Sig. .000 a. Kruskal Wallis Test
60
Multiple Comparisons
Tukey HSD
Dependent
Variable (I) perlakuan (J) perlakuan
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound jam_ke1 CMC Na Suspensi allopurinol 10
mg/kg BB
-12.54000*
1.61180 .000 -17.3631 -7.7169
suspensi EEDP 200 mg/kg BB
-6.20000* 1.61180 .008 -11.0231 -1.3769
suspensi EEDP 400 mg/kg BB
-7.97600* 1.61180 .001 -12.7991 -3.1529
suspensi EEDP 600 mg/kg BB
-8.09600* 1.61180 .001 -12.9191 -3.2729
Suspensi allopurinol 10 mg/kg BB
CMC Na 12.54000* 1.61180 .000 7.7169 17.3631 suspensi EEDP 200
mg/kg BB
6.34000* 1.61180 .007 1.5169 11.1631
suspensi EEDP 400 mg/kg BB
4.56400 1.61180 .069 -.2591 9.3871
suspensi EEDP 600 mg/kg BB
4.44400 1.61180 .080 -.3791 9.2671
suspensi EEDP 200 mg/kg BB
CMC Na 6.20000*
1.61180 .008 1.3769 11.0231 Suspensi allopurinol 10
mg/kg BB
-6.34000* 1.61180 .007 -11.1631 -1.5169
suspensi EEDP 400 mg/kg BB
-1.77600 1.61180 .804 -6.5991 3.0471
suspensi EEDP 600 mg/kg BB
-1.89600 1.61180 .764 -6.7191 2.9271
suspensi EEDP 400 mg/kg BB
CMC Na 7.97600* 1.61180 .001 3.1529 12.7991 Suspensi allopurinol 10
mg/kg BB
-4.56400 1.61180 .069 -9.3871 .2591
suspensi EEDP 200 mg/kg BB
1.77600 1.61180 .804 -3.0471 6.5991
suspensi EEDP 600 mg/kg BB
-.12000 1.61180 1.000 -4.9431 4.7031
suspensi EEDP 600 mg/kg BB
CMC Na 8.09600* 1.61180 .001 3.2729 12.9191 Suspensi allopurinol 10
mg/kg BB
-4.44400 1.61180 .080 -9.2671 .3791
suspensi EEDP 200 mg/kg BB
1.89600 1.61180 .764 -2.9271 6.7191
suspensi EEDP 400 mg/kg BB
.12000 1.61180 1.000 -4.7031 4.9431
jam_ke2 CMC Na Suspensi allopurinol 10 mg/kg BB
-32.60400* 3.05797 .000 -41.7546 -23.4534
suspensi EEDP 200 mg/kg BB
-20.47200*
3.05797 .000 -29.6226 -11.3214
suspensi EEDP 400 mg/kg BB
-26.46400* 3.05797 .000 -35.6146 -17.3134
suspensi EEDP 600 mg/kg BB
-27.98600* 3.05797 .000 -37.1366 -18.8354
Suspensi allopurinol 10 mg/kg BB
CMC Na 32.60400*
3.05797 .000 23.4534 41.7546 suspensi EEDP 200
mg/kg BB
12.13200* 3.05797 .006 2.9814 21.2826
suspensi EEDP 400 mg/kg BB
6.14000 3.05797 .298 -3.0106 15.2906
suspensi EEDP 600 mg/kg BB
4.61800 3.05797 .568 -4.5326 13.7686
suspensi EEDP 200 mg/kg BB
CMC Na 20.47200*
3.05797 .000 11.3214 29.6226 Suspensi allopurinol 10
mg/kg BB
-12.13200*
3.05797 .006 -21.2826 -2.9814
suspensi EEDP 400 mg/kg BB
-5.99200 3.05797 .320 -15.1426 3.1586
suspensi EEDP 600 mg/kg BB
-7.51400 3.05797 .141 -16.6646 1.6366
suspensi EEDP 400 mg/kg BB
CMC Na 26.46400* 3.05797 .000 17.3134 35.6146 Suspensi allopurinol 10
mg/kg BB
-6.14000 3.05797 .298 -15.2906 3.0106
suspensi EEDP 200 mg/kg BB
5.99200 3.05797 .320 -3.1586 15.1426
suspensi EEDP 600 mg/kg BB
-1.52200 3.05797 .987 -10.6726 7.6286
suspensi EEDP 600 mg/kg BB
CMC Na 27.98600*
3.05797 .000 18.8354 37.1366 Suspensi allopurinol 10
mg/kg BB
-4.61800 3.05797 .568 -13.7686 4.5326
suspensi EEDP 200 mg/kg BB
7.51400 3.05797 .141 -1.6366 16.6646
suspensi EEDP 400 mg/kg BB
1.52200 3.05797 .987 -7.6286 10.6726
jam_ke3 CMC Na Suspensi allopurinol 10 mg/kg BB
-58.60200*
4.58119 .000 -72.3106 -44.8934
suspensi EEDP 200 mg/kg BB
-44.55800* 4.58119 .000 -58.2666 -30.8494
suspensi EEDP 400 mg/kg BB
-54.80000*
4.58119 .000 -68.5086 -41.0914
suspensi EEDP 600 mg/kg BB
-56.85000* 4.58119 .000 -70.5586 -43.1414
Suspensi allopurinol 10 mg/kg BB
CMC Na 58.60200* 4.58119 .000 44.8934 72.3106 suspensi EEDP 200
mg/kg BB
14.04400*
4.58119 .043 .3354 27.7526
suspensi EEDP 400 mg/kg BB
3.80200 4.58119 .918 -9.9066 17.5106
suspensi EEDP 600 mg/kg BB
1.75200 4.58119 .995 -11.9566 15.4606
suspensi EEDP 200 mg/kg BB
CMC Na 44.55800*
4.58119 .000 30.8494 58.2666 Suspensi allopurinol 10
mg/kg BB
-14.04400* 4.58119 .043 -27.7526 -.3354
suspensi EEDP 400 mg/kg BB
-10.24200 4.58119 .207 -23.9506 3.4666
suspensi EEDP 600 mg/kg BB
-12.29200 4.58119 .092 -26.0006 1.4166
suspensi EEDP 400 mg/kg BB
CMC Na 54.80000* 4.58119 .000 41.0914 68.5086 Suspensi allopurinol 10
mg/kg BB
-3.80200 4.58119 .918 -17.5106 9.9066
suspensi EEDP 200 mg/kg BB
10.24200 4.58119 .207 -3.4666 23.9506
suspensi EEDP 600 mg/kg BB
-2.05000 4.58119 .991 -15.7586 11.6586
suspensi EEDP 600 mg/kg BB
CMC Na 56.85000*
4.58119 .000 43.1414 70.5586 Suspensi allopurinol 10
mg/kg BB
-1.75200 4.58119 .995 -15.4606 11.9566
suspensi EEDP 200 mg/kg BB
12.29200 4.58119 .092 -1.4166 26.0006
suspensi EEDP 400 mg/kg BB
62
jam_ke1
Tukey HSDa
perlakuan N
Subset for alpha = 0.05 1 2 3 CMC Na 5 -2.6700
suspensi EEDP 200 mg/kg BB 5 3.5300
suspensi EEDP 400 mg/kg BB 5 5.3060 5.3060
suspensi EEDP 600 mg/kg BB 5 5.4260 5.4260
Suspensi allopurinol 10 mg/kg BB
5 9.8700
Sig. 1.000 .764 .069
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.
jam_ke2
Tukey HSDa
perlakuan N
Subset for alpha = 0.05 1 2 3 CMC Na 5 -10.4600
suspensi EEDP 200 mg/kg BB 5 10.0120
suspensi EEDP 400 mg/kg BB 5 16.0040 16.0040
suspensi EEDP 600 mg/kg BB 5 17.5260 17.5260
Suspensi allopurinol 10 mg/kg BB
5 22.1440
Sig. 1.000 .141 .298
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.
jam_ke3
Tukey HSDa
perlakuan N
Subset for alpha = 0.05 1 2 3 CMC Na 5 -27.1040
suspensi EEDP 200 mg/kg BB 5 17.4540
suspensi EEDP 400 mg/kg BB 5 27.6960 27.6960
suspensi EEDP 600 mg/kg BB 5 29.7460 29.7460
Suspensi allopurinol 10 mg/kg BB
5 31.4980
Sig. 1.000 .092 .918
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.
Mann-Whitney Test
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 CMC Na 5 3.00 15.00
Suspensi allopurinol 10 mg/kg BB
5 8.00 40.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 CMC Na 5 3.00 15.00
suspensi EEDP 200 mg/kg BB 5 8.00 40.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 CMC Na 5 3.00 15.00
suspensi EEDP 400 mg/kg BB 5 8.00 40.00
64
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 CMC Na 5 3.00 15.00
suspensi EEDP 600 mg/kg BB 5 8.00 40.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 Suspensi allopurinol 10 mg/kg
BB
5 8.00 40.00
suspensi EEDP 200 mg/kg BB 5 3.00 15.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 suspensi EEDP 200 mg/kg BB 5 3.00 15.00
suspensi EEDP 400 mg/kg BB 5 8.00 40.00
Total 10
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 Suspensi allopurinol 10 mg/kg
BB
5 7.30 36.50
suspensi EEDP 400 mg/kg BB 5 3.70 18.50
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U 3.500 Wilcoxon W 18.500
Z -1.886
Asymp. Sig. (2-tailed) .059 Exact Sig. [2*(1-tailed Sig.)] .056a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 Suspensi allopurinol 10 mg/kg
BB
5 7.00 35.00
suspensi EEDP 600 mg/kg BB 5 4.00 20.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U 5.000 Wilcoxon W 20.000
Z -1.567
Asymp. Sig. (2-tailed) .117 Exact Sig. [2*(1-tailed Sig.)] .151a a. Not corrected for ties.
66
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a a. Not corrected for ties.
b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 suspensi EEDP 200 mg/kg BB 5 3.00 15.00
suspensi EEDP 600 mg/kg BB 5 8.00 40.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U .000 Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009 Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties. b. Grouping Variable: perlakuan
Ranks
perlakuan N Mean Rank Sum of Ranks jam_ke4 suspensi EEDP 400 mg/kg BB 5 4.60 23.00
suspensi EEDP 600 mg/kg BB 5 6.40 32.00
Total 10
Test Statisticsb
jam_ke4 Mann-Whitney U 8.000 Wilcoxon W 23.000
Z -.940
Asymp. Sig. (2-tailed) .347 Exact Sig. [2*(1-tailed Sig.)] .421a a. Not corrected for ties.
b. Grouping Variable: perlakuan
