^ p f H l i i i CtfU KHOA HOC
THUC TRANG ROI NHIEU TAM TRI CUA SINH VIEN Y DA KHOA NAM THUf 2, TRUdNG DAI HOC Y DUOC THAI BINH NAM 2 0 1 7
Nguyen Van Thjnh', Nguyen Thj Minh niilctng', VQ Duto Anh<, Ha Thu Nguyet^
T O M TAT:
Sau khi tian hanh d i l u tra 300 sinh vien nam thu 2 (Khoa 45) tai Trudng Dai hpc Y Dupe Thai Binh nam 2017, chiing tdi thu dupe k i t qud nhu sau: Ty le sinh vian Y da khoa nam thd 2 cda Trudng Dai hoc Y Duoc Thai Binh CO hian tupng r6i nhilu tam tri chilm 88,3%., trong do: nhom sinh vien cd r l i nhilu d mdc do trung binh chiem 44,1%; nhdm sinh vien eo rdi nhieu d mdc dp nhe chilm 31,0%; nhdm sinh vian co r l i nhieu d mdc dp nang chiem 24,9%.. Nhdm sinh vien cd hpc lye trung binh cd rli nhieu tam tri Id 35,0%i vd hpc luc khd c6 ty le r l i nhieu tdm tri Id 30,6%o. Sinh vien d tro ben ngodi co hian tupng roi nhieu tam tri cao (chilm 74,0yo) so vdi nhdm sinh viSn kh6ng d trp (d nhd, nhd ngudi quen hay d ky tue xd).
TIT khoa: Rdi nhieu tam tri, sinh vien nam thu 2
ABSTRACT:
T H E SITUATION O F M E N T A L D I S O R D E R S OF T H E S E C O N D Y E A R STUDENTS AT T H A I BINH M E D I C I N E AND P H A R M A C Y UNIVERSITY IN 2017
After conducting a survey of 300 second year students (45"" course) at Thai Binh University of Medicine andPharmacy m 2 0 1 7 , we obtained the followmg results:
Thai Binh Medico-mental Medicine accounted for 88,3%
of students with mental disorders, in which students with moderate disability accounted for 44,1%.; group of students with mild disturbance accounted for 31.0%;
Students with severe disturbances accounted for 24,9%.
The average student leammg disability group had 35,0%
mental disability and 30,6%) had a mental retardation rate of 30,6%. Students staying outside have a high level of mental confusion (74,0%.) compared to non-homestay students (at home, at home, or m a dormitoiy).
1. Khoa Y te Cong cpng, Trirdng DH Y Duyc Thai Binh 2. T r u d n g C a o ddng S u p h a m Thdi Binh
Keywords: Mental disorders, the second year students.
I. DAT VAN DE
Theo dinh nghTa ve sue khoe ciia T6 ehdc Y t l t h i gidi (WHO) sue khoe tdm tii Id m6t b6 phdn tao nen sdc khde d moi chiing ta vd r l i nhilu tdm tii (mental disorders) bilu thi su lech l^c v l sdc khoe tam thdn. Mdt so nghian cuu da chi ra rdng, sinh vien theo hpc nganh Y mac phdi ede v i n de ve sue khoe tam tri cao hon sinh vien cac ngdnh khdc. Nghien cuu eua Trudng Dai hpc Y Hd Ndi nam 2013 tren 8 trudng d^i hpc y trong cd nude, kat qud cho thay co 43,0% trong s l 2.099 sinh vien co ddu hieu tram cam. Trong d6, co 2 3 % trdm cdm nhp va 20%.
cd tha tram cdm nang. O Viet Nam, den thdi diam ndy, chua CO nhieu nghien cuu ve tinh trang rdi nhiau tdm tri d smh vien dang theo hpc ngdnh y da khoa ndi chung vd d l i vdi sinh vian dang hoc nam thd 2 noi rieng. Tu nhirng ly do tran, chiing toi tien hdnh'W^Ai^n cuu thuc trpng roi nhiiu tam tri cua sinh vian y da khoa nam thd 2, Trudng Dai hpc Y Dupe Thdi Binh ndm 2017", nham muc tieu:
Md td thuc trang roi nhieu tdm tri cua sinh vien y da khoa ndm thu 2, Tnrdng Bai hpc Y Dupe ThdiBinh ndm 2017.
H. B 6 I T U " Q [ N G V A P H U O N G P H A P NGHlfeN C U t i
2.1. Doi tupfng nghiin curu: Sinh vien he chinh qui, ngdnh Y da khoa nam thd 2, Trudng Dai hpc Y Dupe Thai Binh.
2.2. Thdi gian nghien cuv: Td thang 02 den thang 06 ndm 2017
2.3. Dia diem nghiin ciru: Trudng Dai hpc Y Duoc Thdi Binh
2.4. PhuWigphdp nghiin ciru Thiet ke nghien cuu •
Nghian cdu dupe tien hdnh theo phuong phdp dich te
Ngaynhanbai: 15/01/2018 Ngay phan bipn- 25/01/2018 n Ngay duypt dang: 10/02/2018
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CONGDONGt3018
hoc m6 td vdi cupe dieu tra cat ngang co phan tich, Cd mdu vd chpn mdu
<• Co mdu: Sd dung c6ng thuc dp dyng cho nghian ciiu m6 td cat ngang:
_ z V „ 2 . f ( l - p )
Vdi p = 20%. (Theo kit qua nghien cdu eiia Trung tdm Nghien cdu vd dao tao phat trien cpng dong (RTCCD) nam 2008. Tu c6ng thdc tren tinh dupe: n = 245. Tren thyc tl chiing tii tiln hanh dilu tra 300 smh vian.
• Chpn mau
Tien hanh lap danh sdch todn bp sinh vien da khoa nam thd 2 theo ldp (khda 45 ed 10 ldp) ha chinh quy, lo^i tru sinh vien ngudi nude ngoai. Tian hanh chpn 30 sinh
vien cua m6i Idp tiieo cdch bde tham ngdu nhian doi vdi cdc em smh vien tu nguyen tham gia trong danh sdch.
2.5. Phuongph^ thu thpp thdng tin Trong nghian cuu nay, chdng tdi sd dyng bp cau hoi SRQ20 cua WHO. SRQ bao gdm 20 cau h6i vdi 2 miit tieu lua chpn tra ldi "Co" bode "Khdng". Nan trd ldi "Co"
dupe 1 diem; trd ldi "Khdng" duoc 0 diam
Cdch xdc dinh mdc dp r6i nhieu tdm tn cy tha nhu sau:
• Dudi 6 diam : Kh6ng co r6i nhieu tam tri
• Tu 7 - 11 diem : Roi nhiau tdm tri muc dp nhe
• Tu 12-16 diam: R6i nhieu tdm tri muc dp trung binh
• Tu 17 - 20 diem: Rdi nhieu tdm tri muc dp nang ni. KET QUA NGHliN CiW
Biiu dd 3.1: Ty li RNTT cua ddi tupng nghiin cuu
Kit qud bilu dd 3.1 cho thdy: c6 88,3%. sinh vien sinh vien nam 2 ngdnh Y da khoa dupe phdng vdn. Chi c6 duoc xdc dinh Id ed rdi nhieu tam tri trong tdng sd 300 11,7% sinh vian khdng ed rdi nhieu tam tri.
Biiu do 3.2: Muc dp RNTTd doi tupng NC
a Nhe
QTrung binh
• Nang
78
S 6 2 (43) - Thing 03-04/201S Website yhoccongdong vnNI^HilM CUfU KHOA HOC
Bieu dd 3.2 cho bilt; trong ting so 265 sinh vien Y da 24,9%. rdi nhiau d muc dp njng, 44,1 % sinh vien rdi nhilu )a nam thu 2 dupe xdc djnh Id co rdi nhilu tam tri, co d mdc dp trung binh va 31,0%i rdi nhieu d muc dp nhe.
Bdng 3.1, Co cdu vi hipn tuong RNTT theo gidi tinh cda sinh vien GIAI tinb
Nam NS
TSng
S d l u ^ g 81 184 265
Til|C%) 30,6 69,4 100,0 Bang 3.1 cho tiiay ti-ong tdng sd smh vien tiiam gia hodn todn phii hpp vi ddi tupng nghien cdu chilm da sl Id nghien cuu cd RNTT thi sinh vien nu chilm 69,4%, nam nd, nlu tinh theo s6 hpc thi ty Ip cd RNTT theo gidi tinh sinh vien ed rli nhilu tam tin vdi ty 1^ 30,6%o; kit qud nay Id tuong duong nhau.
Bdng 3.2. Cff cdu v
Hgclirc Gioi
Kha Trung binh Trung binh yeu Kem
Tong
' hien tiring RNTT theo hpc lire cua sinh vien Co RNTT S6 lu'9lig
28 81 93 45 18 265
TilfC%) 10,6 30,6 35,0 17,0 6,8 100,0
Bang trgn cho th^y h i ^ tut?ng RNTI xudt hien a tit ci 35,0%; nh6m CO l\rc Idia chiim 30,6%; ke dgn II nhom sinh sinh vien co kk qua hpc t|ip khac nhau. Trong s6 do, nhom viSn c6 hgc l\rc bung binh y§u la 17,0%, hoc lye gi6i chiem sinh vien co hoc lue hung bmh ehiem ty le eao nhat vol 10,6%;sinhvieneohpclircy6uchi6mtyl§thapnhatC6,8%).
Bdng 3.3. Ca cdu ve hien tuffng RNTT theo nffi & cda sinh vien
Noi ii' cua slnh vien 6 bo
0 nha ngum quen d Id tuc xa 6 nha
Tong
Co RNTT S6 lirgng
196 6 50 13 265
Ty 1| % 74,0 2,3 18,9 4,8 100,0
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Bdng 3.3 cho thdy trong s6 nhirng sinh vien cd rdi nhieu tam tri, phdn d6ng d trp ben ngodi (chiem 74,0%), mdt bp phan d ki tue xa (18,9%), so it sinh vien sdng ciing gia dinh (4,8%) hoac d nhd ngudi quen (2,3%). Tren thuc ta ve nai d cda sinh vien nha tnrdng, da so la d trp do chua du ch6 d trong ky tiic xd, t^i k^ tuc xa eiia nha trudng chi du bd tri cho nhung sinh vian cd hodn cdnh kho khan, gia dinh chinh sdch, do do sinh vian phdi trp d ngodi nan ciing CO nhiau phiic tap ddn den nhung dp luc doi vdi sinh vian,
IV. BAN LUAN
Trong nghien cuu nay, chiing toi chpn nguong dinh biat Id > 7, nghia Id neu sinh vien ndo co t6ng s6 diem td 7 trd ISn thi dupe xep vdo nhom eo rdi nhieu tdm tri.
Theo d6, cd 88,3% sinh vien Y da khoa nam thu 2 dupe xac djnh Id c6 rdi nhieu tdm tri. Ty le nay cao hon so vdi nhirng da tdi chung t6i dupe biet vd tham khdo, dac biet cao han so vdi mpt sd nghien cuu nuoc ngodi tren d6i tupng thanh nian (bao gdm cd nhdm sinh vian dai hpc vd cao dang) cung sd dung bO c6ng cu SRQ20 cda WHO cho ty Ip rli nhilu tdm ti-i dao dpng tii 17-35%
(tuy thupc vdo nguong dinh biat). Nghian cdu cua Trdn Ngpc Ddng, Dd Van Dung, Huynh Ho Ngpc Quynh ciing dung bp cong cy SRQ20 de xdc dinh ty Ip roi nhilu tdm tri cua sinh vian khoa Y te e6ng cpng, Dai hpc Y Dupe TP Hi Chi Minh nam 2010 cho kit qud: 58,47%
vdi nguong dinh biet Id > 8 [15]. Mpt nghien cdu khdc tiln hdnh d Thdi Binh nam 2012 h'en hpc sinh THPT su dyng bp cdng cy SDQ25 dl chdn doan sdng Ipc rli nhieu tdm tri tran doi tupng 4-16 tuli cho thdy: 24,2%.
hoc sinh co van da vl sdc khde tam tri [6]. Nghian cuu mdi nhdt tien hdnh trSn quy m6 8 trudng, khoa Y trong cd nude (2014) dli vdi 2099 sinh vian nam thu nhat, thd ba va thd nam chi ra: c6 43,2% doi tupng nghian ciru tiing trdi qua nhirng dau hi^u tu trdm cam nhe trd len (theo thang do ti-dm cam CES-D); cy thi cd 23% co tripu chung tram cam td nhe din tnmg binh (muc dilm CES-D tii 16 dan 21), ty Ip sinh vian roi vao mdc trdm cam nang la 20,2% (dilm CES-D >21) [2]. Sd di kit qud nghien cdu cua chiing t6i cao vupt trpi nhu tran co the xudt phdt tu khai niem rli nhilu tam tri Id mpt each nhin nhdn mdi vl tinh tr^ng sue khde tam thin theo hudng dy phdng. Vdi rdi nhieu tdm tri, pham vi dh c^p dan s6 d6ng ban, bieu hipn benh d mdc dp nhe ban va nhu vdy nhilu khd ndng ty le rli nhilu tam tii (trong ddn so) se cao hon so vdi ty le stress hay ty la mdc cac dang roi loan tdm than khdc (tam thdn phdn li^t, rdi lo^n trdm cdm, rli lo^n lo du ..). Nghian cdu tiran dli tupng sinh vian Y da khoa co nhung dilm chung vd nhirng dilm khac bipt nhdt dinh vdi sinh vien Y tl cong cpng vl chuong trinh, thdi gian, dp luc hpc tap... Vi
vdy, mac du cung sd dyng bp c6ng cy SRQ20, ty le sinh vien cd rdi nhilu tdm tri trong nghien cdu cua chung t6i cao hon so vdi kit qua cuaTrdn Ngpc Dang, Dd Van Diing, Huynh Hd Ngpc Qu^mh [7].
Xet ve muc dp rdi nhiau tdm tri, chdng t6i ciing chung quan diem vdi cdc tac gid eua Khoa Y te c6ng cong, D^
hpc Y Dupe TP H6 Chi Minh, chia miic dp roi nhieu thdnb ba lo^i: nhe (tu 7 den II diem), trung bmh (td 12 din 16 diam) va muc dp nang (tu 17 den 20 diam). Qua dilu tra, ty le sinh vian co rdi nhieu d mdc dp trung binh chilm cao nhdt (44,1 %), tiep dan la mdc dp nhp (31,0%), muc dp nling chiem ty le thdp nhat (24,9%), Nhu vdy, da phdn sinh viSn CO rdi nhieu tdm tri d muc dp td nhe den trung binh, Ket qud ndy ciing phil hpp vdi nghien cdu cua Trdn Ngpc Dang, Do Van Diing, Huynh Hd Ngpc Quynh [7].
Trong s6 sinh vien co r6i nhieu tam tri, ti le sinh vien nu gdp hon hai Idn smh vien nam (69,4% vd 30,6%), dieu nay ciing phu hpp vdi ty le nam - nii dupe nghien cuu theo phdn tich ve sd hpc, do dli tupng la nu gdp hon hai Idn ddi tupng nam. Tuy nhian nlu xet riang tdng gidi, ty le r6i nhieu tdm tri cua nam vd nii la tuang duong nhau vd deu d muc rdt cao (88%), tuong ding vdi ty le r6i nhiau chung. Su chenh Ipch vl rli nhilu tim tri giira hai gidi trong nghien cdu cua chung t6i ciing phu hpp vdi ket qud eua Tran Quynli Anh,Michael P. Dunne, Luu Ngpc Hoat tren 8 tnrdng, khoa Y cua ca nude (2014) [2].
Trong nghien cuu cda cdc tdc gia D^i hpc Y Dupe TP H6 Chi Minh (2010), khdng eo sy khac biet co y nghTa tiling ke ve ty le roi ohieu tam tri d nam vd nii, tuy nhien ed sy khdc biat kha rd rdng theo sinh vien cdc nam hpc vd ty le cao nhat ciing thupc ve sinh vien nam thu hai [7].
Ty le rdi nhieu tdm tri cao thupc ve nh6m sinh vien cd hpc luc kha vd trung binh (tdng sl 65,6%) tiong khi ty le nay thdp d nhom sinh vian hoac c6 hpc luc gidi (10,6%) hoac cd hpc lyc trung binh ylu (17,0%.), kem (6,8%); ket qua ndy phu hpp vdi nghien ciiu cua tdc gid Tran Ngpc Dang khi nghian cuu tren doi tupng la sinh vian ngdnh YTCC nam 2011 [7], Thuc te dli vdi nhiing sinh vien cd hoc luc gidi nhung neu hi thi lai hoac dang gidi nhiing do hpc lye xu6ng miing thap hon de bi RNTT hem la nhung sinh vian co hpc lyc ylu. Nhu vay khi ngudi hpc dat dupe kit qud hpc tap d mdc eao ho$c c6 ket qua d mdc thdp nhdt, hp khdng cd nhieu bilu hi^n cua lo au, bu6n ba, mpt mdi so vdi nhiing sinh vian co hpc lyc d mdc trung binh, kha. Ddi vdi sinh vien Y da khoa nam thd hai eua Trudng Dai hpc Y Dupe Thai Binh la khda ddu tien dp dung chuong hinh ddo tao theo hpc chi tin chi. Theo do khoang dilm dl phdn chia hpc l\rc khdc vdi hinh thdc ddo tao theo nian chi. Dl d^t hpc l\rc gidi, sinh vian cdn cd dilm tiung binh chung hpc tdp cdc mon td 8,5 din 10; hpc lyc kha: td 7,0 din 8,4; hpc
Qf\ j S 6 2 (43) - Thing 03-^4/2018 I Websile' yhoccongdong vn
N | ( H I E N CUfU KHOA HOC
lire mmg binh: tu 5,5 din 6,9; nlu dilm t6ng kat td 4,0 den 5,4, sinh vian se bi xip lo^i hpc lyc trung binh yen va dudi 4,0 se xIp loai kem. Sinh vian dat hpc lye gidi dudng nhu hp hdi Idng vdi kit qud hpc tap cda minh; vdi nhiing sinh vien xIp loai hpc lyc trung binh yeu, kera, qua phdng van chiing tdi dupe bilt hp khdng coi kat qud hpc X$.p la mpt dp lyc qua Idn vdi minh.
Theo ket qud bang 3.3, ty Ip sinh vien cd roi nhilu tdm tri cao nhat Id nhom thua nhd d trp (74,0%), mpt bp ph^n nho d ki tuc xa cho biet co nhiing biau bien cua roi nhieu tdm tri (18,9%.) va rdt it ngudi e6 rli nhilu khi song ciing gia dinh (4,8%.) hoac ngudi quen (2,3%.).
Dieu ndy cd the ly gidi td viae sinh vien khi phdi sing xa nhd, ban canh vi§c hoe tdp con phdi ty lan ke hoach cho cupe sing sinh boat hang ngdy, chd dpng trong ehi tiSu vdi ngu6n kinh phi chu cap td ngudi thdn, gia dinh, doi hoi cac em phdi ed sy thich nghi vdi mdi trudng mdi vd lam chu cupe sdng cda minh. O trp ben ngoai cho cac em su tu do nhdt dinh trong smh boat, tuy nhian tiam dn nhieu cam dd, nguy hiem ban so vdi mdi trudng ky tuc xd, va chae chan kh6ng eo dp an toan nhu d ciing gia dinh, ngudi thdn. Kit qud ndy cua chung t6i ciing tuong dong vdi nghian cdu cua Trdn Quynh Anh, Michael P.
Dunne, Luu Ngpc Hoat [2].
V. KET LUAN
Ty le sinh vian Y da khoa nam thu 2 eua Trudng Dai hpc Y Dupe Thdi Binh cd hien tupng rli nhilu tam tri chiem 88,3%, trong do: nhom sinh vien co rdi nhilu d mdc dp trung binh chiem 44,1%; nh6m sinh vien co rdi nhieu d mdc dp nhp chiam 31,0%; nhdm sinh vian co rdi nhieu d mdc dp n^ng chilm 24,9%.
Nhom sinh vian co hpc lyc trung binh c6 RNTT Id 35,0% va hpc luc khd cd ty Ip RNTT Id 30,6%;
Sinh vian d trp ben ngodi e6 hien tupng RNTT chilm 74%. cao hon so sinh vian khdng d trp.
VI. KHUYEN NGHI
Nhd trudng, Phdng Qudn ly Ddo tao dai hpc cdn xem xet kh6i lupng hpc tdp cho dli tupng sinh vien Y da khoa nam thu hai theo hinh thdc ddo tao chi tin chi, trdnh sy qud tdi, dam bao chdt lupng d^iy vd hpc.
Neu cao vai trd ciia nhd tnrdng, t6 phuong phdp, giao vian chd nhi^m, Dodn Thanh nian, Hpi sinh vian, cac cdu l^c bp dk t^o cho sinh vien co nilm tin trong mdi trudng
"d^i hpc", t^o moi quan ha gdn ket giua ede sinh vien eting nhu hinh thanh cho ngudi hpc cdc ki nang, nhat Id ki nang mem de doi pho vdi hien tupng rdi nhieu tam tri.
I KHAO
1. WHO (i994), A USER'S GUIDE TO THE SELF REPORTING QUESTIONAIRE (SRQ).
2. Trdn Quynh Anh, Michael P. Durme, Luu Ngpc Hoat (2014), Su thoai mdi, dau hipu tram cdm vd y dinh tu td ciia sinh vian cac trudng dai hpc y tai Vipt Nam, Tap chi YDupe hoc l^erA'a/n-B6Yta, sl 6, thang 3/2014
3. Le Thuc Due, Nguyen Phuang Ngoc, T r ^ Minh Chau, Nguyen Van Tiln, V6 Thanh Son (2008), Chuong trinh nghian cdu "Nhirng cupe ddi tre tho": Khdo sdt vdng 2 tai Viet Nam, Bdo cdo quIc gia vong 2 cda Trung tam Nghian cdu vd Ddo tao phdt trien cpng ddng (RTCCD).
4. Le Thi Kim Dung, Ld Thi Budi, Dinh Ddng Hde (2007), Nghien ciru mpt so yeu to dnh hudng sue khde tdm thdn ciia hpc sinh d mpt so trudng trung hpc co sd. Hpi thao can fliiep va phdng ngua cdc vdn de sue khde tinh tiian tre em Vi^t Nam.
5. Nguyln C6ng Khanh, Nguyen Thi Hdng Phuong(2005), Su dung thang lo du Zung di tim hiiu thuc trang lo du d hpc sinh THPT tren dia bdn thdnh pho Hd Ndi.
6. Ph^m Thi My Hanh, Le Thi Lipu (2011), Xac dmh ty Ip mdc dau hieu h-dm cdm bdng tiiang CES-D vd cac ylu
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HOC ^ „ ^_
OONG DONG 2018
t l gdy sang chdn tdm ly d sinh vien nam thu nhdt nien hpc 2009-2010 trong cdc trudng cao dang, dai hpc thupc thdnh phd Thdi Binh, Bi tdi khoa hoc cdp co sd Trudng D^i hpc Y Dupe Thdi Binh.
7. Trdn Ngpc Dang, D6 Van Diing, Huynh H6 Ngpc Quynh (2011), Ty le r l i nhilu tdm tri vd eac yeu td lien quan cua sinh vien khoa Y t l c 6 n g cpng, Dai hpc Y Duoc TP.HCM nam 2010, Tpp chi Nghien cicu Y hpc TP.HCM, t^^ 15, Phu ban sd 1,2011.
- o O o -
Gldl THIEU PHUONG PHAP M d i PHAN LAP GEN lU MAU TU NHIEN DE TAO RA PROTEIN TAI TO HOP HOAT TINH
T 6 M TAT:
Td culi thi ky XX din nay, sy phat triln nhanh trang tiiilt bi, ky tiiuat hien dai, phli hpp vdi cdng nghe th6ng tin da cho phap don gidn hoa cong viec trong phong thi nghiem sinh hpc vd vdn tao ra dupe nhiing sdn phdm sinh hoc co gia tii cao, Cac till he mdy giai trinh ty ADN ra ddi, giup cung cdp khoi lupng dii heu khong Id ede genom sinh vdt, d GenBank, dac biet cd genom cua nhiau loai vi khudn. Vi vay, viec sdng Ipc '^nzyme gidi ban da nit ngan qud trinh nghian ciiu chi CO J bude: budc 1 tim gen dich in silico ma cho enzyme gidi ban gid djnh, budc 2 phdt hien cac gen tuong ty trong man thian nhian, bude 3 clon gen tuong ty dd de CO sdn pham tdi td hpp la mpt enzyme gidi ban c6 hoat tinh.
Su thdnh cong cua qud trinh nay dupe chiing minh th6ng qua cdc thdnh tuu khoa hpc kit hpp lai vdi nhau. Quy trinh nghien ciiu dupe thyc Men true tiap tren mdu thian nhian nhdm t^o ra san pham tdi t6 hpp, chi g6m bai khdu in silico vd in vitro. Hian nay, nhung ky thuJit phde t£ip trd thanh don gidn khi cdc thiet bi cua phong thi nghiem sinh hpc phdn td dupe ket n6i vdi cdng nghe th6ng tin.
T u khoa: Tm sinh hpc; enzyme gidi ban; PCR; clon gen.
SUMMARY:
A N E W M E T H O D T O OBTAIN EXPRESSED RECOMBINANT PROTEINS F R O M ENVIRONMENTAL DNA GENES ALIKE A TARGET IN SILICO GENE
Since the end of tiie XXth centuiy, the recent development of equipment and methods for the biology
Le Thj Kim lUyen', Bach Khanh Hoa'
research, in association with the information technology has facilitated the laboratory research in getting high value biological products. Thanks to the sequencing automation, an enormous quantity of genomic data has been collected in GenBank, especially genomes of many bacterial species. On this b ^ i s , the screening of restriction enzymes can be made with a shortened procedure, beginning with the finding in silico of a target gene coding for a putative protein, then detecting the alike genes in natural samples, finally cloning the genes to get an active restnction enzyme. The success of the method is demonstrated through efficient methods that were combined with each other. The research procedure is performed directly on natural samples in order to get final recombinant products from in silico to in vitro research parts. Nowadays, former complicated techniques have been made easier m molecular biology laboratones equipped with information technology.
Keywords: Bioinfoimatics; restiiction en2ymes;
PCR; gene cloning.
L D A T V A N D E
C6ng ngh§ thdng tin Id mdt cupe cdch mang da ddy m^nh n h i l u ITnh vuc khoa hpc trong dd co nghien cuu sinh hpc. Su phoi hpp giira cdng nghp th6ng tm vd c6ng nghe sinh hpc h i tro cho sy tian bp trong nghiSn cdu sinh hpc, cdc p h u o n g phdp trudc day phuc tap nay da trd thdnh d a n gidn vd dupe sd dyng mot each r6ng rdi. T d khi ed nhung thilt bi hien dai nhu may giai
1. Trudng Dai hpc Thang Long, Ha Npi
S
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