No 14_Dec2019|S6 1 4 - T h a n g 12 nam 2019|p.90-93
TAP CHI KHOA HOC DAI HOC TAN TRAO ISSN: 2354-1431
http://tckh.daihoctantrao edu.vn/
Anh hu-ong ciia mot so den Led do Rang Dong san xuat den su- sinh trirffng va phat trien cua choi giong Bach dan P N C T 3 va giong Keo lai BVIO trong dieu kien in vitro
Pham Thi Hanh"', Pham Thi Mai Trang", Nguyen Vdn Gidp""TrudngDai hoc Tdn Trdo Email: maitrang.bvtvtq@gmail com
Thong tin bai viet
Ngdy nhdn bdi:
18/10/2019 Ngdy duyet ddng 10/12/2019
Tir khda:
Den Led, Bach ddn PNCT3, Keo lai BVIO, In vitro.
Tom tat
Anh sang, mdt trong nhQng yeu td quan trong cho su sinli trudng va phat tnen cua thuc vat, nd tham gia vao nhidu qua trinh sinh iy cua thuc vat, Irong dd, cd qua trirdi quang hap, qua trinh quang phat sinli hinh thai, tinli hudng sang Trong nghien cQu lac ddng cua imh sang den su sinli trudng va phat tnen cua choi Bach dan PNCT3 vii Keo lai BVIO, sii dung cac loai den chieu sang khac nhau do Rang Ddng sim xuat nhu den Led anh sang trang 16w, den Led anh sang trang lOw, den Led anh sang vang 16w, den Led anh sang vang lOw va cdng thuc doi chung sQ dung den huynh quang 36w Ket qua, sau 4 luan nudi cay, cdng thQc sii dung loai den Led imh simg tring 16w chdi gidng Keo lai BVIO cd khii nang sinh trudng, phat trien dat cao nhal, Ca 4 loai den Led do Rgng Ddng san xual sQ dung hong nghien ciiu nay deu khong phu hop vdi sQ sinh trudng phat tnen cua chdi gidng Bach dan PNCT3
1. fiat van de
Nudi ciy md td bao thuc vat, cdn goi la nudi cay in vitro la phuang phap giup nhan gidng thuc vat mang nhQng dac didm di huyen mong muon va sach benh Trong vai thap ky qua viec ung dung phuong phap nudi ciy md te bao thuc vat vao nhan gidng da hd ndn phd bidn Theo huydn thdng, U'ong nuoi ciy md id bao thuc vat con ngudi kiem soat su sirdi trudng phat Uidn cua cay thdng qua dieu chinh didu kien nudi cay nhu Mdi hudng dmh dudng, chat dieu hda smh Uudng, ndng dp CO^ va cac phuang phap xu ly hda hoc khac nhau (T Kozai,1995) hong khi dd anh sang nhan tao cho cay quang hop diudng sQ dung la den huynh quang phat ra ardi sang Q-ing, do dai budc sdng rdng 400-700rmi vdi cudng do cd dinh Nhung diuc ra, su sinh hudng va phat tnen eiia thuc vat lai duoc dieu hda bing nhidu te bao cam quang khac nhau duoc kich hoat bdi cac hat proton eua phd anh sang xanh lam, dd va gan dd (Doim Suy NghT,2006)
Mpt luong Idn pho anh siing ma den huynh quang phat ra thuc vat khdng su dung duoc, Ben canh dd, su chieu xa qua mQc cung ardi hudng den su quang hpp ciia diuc vat, Ngpai ra sQ lang phi nang luong do tao ra cac hat proton cd budc sdng khdng mong mudn va su chidu xa anh sang du thQa lam ton nhidu n3ng luong dudi dang nhidt, Vi vay, den huynh quang khdng phiii iua chpn li tudng de cung cap anh sang cho su quang hap cua diuc vat trong in vitro va can cd mdt ngudn anh sang hieu qua cai thien tdc dd nhan gidng, nang cao chat luang cay gidng va giam chi phi nhan gidng (S D, Gupta,2017), den Led da gdp tdn minh vao dardi sach san pham chieu sang hQu hieu trong nuoi cay md le bao Den Led cd uu diem la cd budc sdng xac dinh, phat ra anh sang mau don sic kidm soat duoc thanh phan quang phd, cudng dd anh sang cao, sinh nhiet diap, tudi tho lau dai va tiet kiem nang luong (Lan Ashdow,20I5) Hidn nay. Cdng ty bdng den phich nudc Rang Ddng da che tao ra duoc mot sd bdng den Led chuydn dung dung Qong nudi cay md vdi cudng do ardi sang va mau sac kliac nhau. Nhung chua cd mpt
P.THanh e / a f No 14_Dec 2019|p 90-93
cdng bd cu the niio viec sQ dung bdng den Led ciia Cong ty bong den phich nudc Rang Ddng Qong nudi cay md te bao cay Keo lai va Bach dan Vi vay, viec nghien cQu anh hudng cua mdt sd loai den Led do Rang Dong san xuat den sirdi Uudng va phat tndn cua cay Bach dan va keo lai nudi cay trong dieu kidn nudi cay md la rat can thiet.
2. Vat lieu, phuffng phap nghien cuii 2.1. Vat liiu nghien ciru
- Chdi Bach dim PNCT3 dupe nudi trong mdi hudng MS (Murashige and Skopg, 1962) cd bd sung BAP 2mg/l, dudng 30g/l, diach 4g/l
- Chdi keo lai ddng BVIO duoc nudi trong mdi hudng MS (Murashige and Skoog, 1962) cd bo sung IBA 2mg/l, BAP 20mg/l, GAs 1 mg/l, dudng 30g/l, thach 4g/l
- Bdng den Led anh sang fring I6w, tring lOw, vang 16w, vang lOw do Cdng ty Rang Ddng san xuat
- Cac dieu kidn nudi cay nhidl dd 25-28'C, do am 50%, thdi gian chieu sang lOh/ngiiy
2.2. Phuang phdp nghien cim
2.2.1. Thi nghiem dnh hudng cua den Led lin su sinh tnrdng vd phdl tnen cua chdi Bach dan PNCT3 trong giai doan nhdn chdi
Thi nghiem dupe thiel ke nham muc dich xac dmh ngudn sang duch hpp cho kha nang lai sinh chdi Bach dan PNCT3 d giai doan nhan chdi. Thi nghiem gdm cd 5 nghiem IhQc, cac bhih nhan chdi Bach dan PNCT3 d3t dudi cac dieu kien anh sang cua 5 den Led khac nhau (Led hang 16w, Led vang 16w, Led h i n g lOw, Led vang lOw, ddi chQng la den huynh quang).
2.2.2. Thi nghiem dnh hu&ng cua den Led len su smh trudng vd phdl tnin cua chdi Keo lai BVIO trong giai doan nhdn chdi
Thi nghiem duoc duet kd nham muc dich xac dinh ngudn sang thich hop cho kha nang tai sinh chdi Keo lai BVIO d giai doan nhan chdi. Thi nghiem gom cd 5 nghiem thQc, cac binh nhan choi va ra re keo lai BVIO dat dudi cac dieu kien anh sang cua 5 den Led khac nhau (Led tring 16w, Led vang 16w, Led U-ing lOw, Led vang lOw, ddi chiing la den huynh quang)
2 2.3. Cdc chi tieu theo doi
Sau 4 hian nudi cay theo doi he sd nhan chdi, chieu cao chdi, U ie chdi hQu hidu (chdi Idn ban 1,5cm), ty le chat kho/hrai, hinh diai chdi.
2 2.4 Phuang phdp xir ly sd lieu
Tat ca cac dil nghidm duoc lap lai 3 lan, thu thap so iieu xu ly bang Microsof Excel va xQ ly didng kd bing
phan mdm SPSS, Su khac biel cd y nghia thdng ke vdi dd tin cay 95%
3. K £ T QUA NGHIEN CUU
3.1. Anh hu&ng ciia den Led tin susinh tru&ng vd phat triin ciia chdi Bach dan PNCT3 trong giai 0ogn nhan choi
Giai doan nhan choi h-ong nudi cay md lii giai doan quan hong nhim tao ra mot sd lupng Idn chdi khde manh, map m^p, du hdu chuin vd chieu cao, sd la de tap cay hoan chinh h-ong giai doan Uep theo
Bdng 1. Ket qua thu nghiem anh hudng cua den Led den kha nang tai sinh chdi Bach dan PNCT3 Urong giai doan nhan chdi
chl^u tint K i i ^ {dil diiiaK)
LedD-lo;
16W Led blag
lOw Ledvta;
l«w Led via;
lOw Hf lit niiHii
Choi 2.9S±0,|k I.S±0,J2' l.Jt0.4S.
I.'l4i0.1' 1.47*0.34'
c W u n o muigUiih 1.33*0.66' 1.4S±0.14' l.43±0.iy 1.43±0.13>
1.43*0.13' Tlli'i'ltil inM iiiru 0.7gifl.0J»
0.1 ±0.01' 0.09±0 02*
0.08*0.01' 0.08*0.01'
Khfll kMkbol lijtmiintri 0,09*0.01' 0.11±0.01' 0.1 it0.01' 0.11*0.01' 0.11*0.01'
HtaUiIlill
xanbmjp iiiJDb.>di
"Cr
•"^
ngoo Ghi chu: "' '"' Irong ciing mpl col the hen su khde biit cd y nghia & muc tin cdy p < 0,05 trong Duncan's test.
TQ kdt qua trong bang 1 cho thay, choi B^ch dim PNCT3 trong didu kien chieu sang cua den Led do Rang Ddng san xuat cho he so nhan choi, chieu cao trung binh cua choi, li Id chdi hQu hieu thap hon so vdi nudi Qong didu kidn den huynh quang
Ben canh do khi nudi chdi Bach dan PNCT3 trong didu kidn anh sang den Led ciia Rang Dpng chdi cin edi, la va ngpn sui callus (hudi 1) Khdi luong khd/khdi luong turn cua chdi Bach dan PNCT3 (0,11) cao han den huynh quang (0,09) Khi xu !y thdng ke cho diay tat ca cac chi heu vd hd sd nhan chdi, chieu cao Irung binh cua chdi, U le chdi hQu hieu, khdi luang khd/khdi luong Uroi ciia chdi Bach dan PNCT3 dudi anh sang den huynh quang ddu khac bidt cd y nghia thdng kd vdi ciic cdng IhQc sii dung den Led cdn lai.
Nhu vay, phd anh sang cua den Led anh sang h i n g I6w, den Led anh sang fring lOw, den Led anh siing vang 16w, den Led anh sang vang iOw do Rang Ddng san xuat khdng phu hop sQ dung hong giai doan nhan tao chdi Bach dan PNCT3
P.THanh etal/fJo 14_Dec 2019|p 90-93
Hinh 1 Anh hudng ciia dieu kidn chieu sang khac nhau Idn su sinh trudng va phat Uidn cua chdi Bach dim PNCT3 A anh sang Led trang 16w, B: anh sang Led trang lOw, C; anh sang den huynh quang
3.2. Anh hu&ng ciia den Led len sif sinh trudng vd phat trien cua choi Keo lai BVIO trong giai doan nhan chM
Bang 2- Ket qua thQ nghiem anh hudng cua den Led len su sinh trudng va phat hidn cua chdi Keo lai BVIO trong giai dpan nhan chdi
q u m g ( d m d n i n i )
L e d s t o i t WW L c d n t n i i
lOw L t d v t e r
I 6 w LtdvAiig
IOw HtU BbiDThAl
2 . 0 6 * 0 . 1 1 '
; . 9 4 * a . i 6 ' . . t t O J = * 3.7T«0.IS*
l , 0 9 ± 0 . l ' Cblru m a
c a * chit
2 8 4 * 0 . 5 3 '
3 , 1 2 * 0 . 1 8 * 2.31*0.17V 2.79*0.18"
l . I - l ± O . J I ' Tl It (bit b O n h l f n
0 . 4 8 * 0 . 0 7 '
0 „ 1 S i 0 . 0 9 ' o,:;*o.oB' 0 . 4 8 * 0 . 0 7 ' 0.1±0.03»
Kbol k b A k b A
0 . 1 * 0 . 0 3 '
O . l 3 i 0 . 0 2 ' 0 . 1 1 * 0 . 0 0 ' 0 0S*O.OO«
D.08±D.00>
HlnhllUU
X a u l L c i v F i A . c k . U
X i n l i . c ^ n o n . l i i | : i i Xniib. iHui.
LUn ttifty
Ghi chii' "''^'''" trong ding mpt col the hien su khde biit cdy nghta & muc tin cdy p<0,05 trong Duncan's test.
Nhin vao bang 2 dd dang nh^n thay he so nhan chpi, chieu cao trung binh cua chdi, ti le chdi hQu hieu, khoi luong kho/khdi lupng tuoi ciia choi keo lai BVIO cao nhat dudi didu kidn anh sang den Led Qang 16w (2,94, 3,12; 0,56, 0,13). Dudi anh sang den Led vang I6w, hd sd nhan chdi, chieu cao Uung binh cua chdi, ti Id choi hOu hieu, khdi luong khd/khdi lupng tuoi chi diap hon anh simg den Led fring 16w mpt chut (2,77, 2,79; 0,48) va cao hon den huynh quang (2,06, 2,84, 0,48) tuy nhien dudi anh sang den Led mau sac vang ti le khoi luong chat khd/khdi luong chat tuai rat thap (0,08), didu nay ly giai ly do tai sao khi nudi cay dudi dieu kien anh sang Led vang quan sat b i n g m i l thudng thay cay
bi hidn tuong thiiy tinh hda Khi xay ra hien tuong thuy tuih hda d giai doan nhan, chuyen sang giai d o ^ t^o cay hoan chinh cay khdng the phat trien t^o thiinh re.
Nhu vay, cudng dp anh sang va mau sic anh sang choi keo lai BVIO hong giai doan nhan chdi dupe nudi dudi dieu kien anh sang Led tring 15w dp Rang Ddng san xuit cd chat lucmg chdi cap nhat hem h i n dpi chQng den huynh quang
3. Ket luan
Vdi nhttng uu diem ciia den Led nhu cd tudi tho cap, cau tnic dac, an loan, phat nhiet it, hdu thu didn nang diap va dac biet cd did phat ra nhieu day budc sang tuy thudc vao cau Uiic ban dan nen den Led cd nhieu didm thuan Ipi trong Qng dung tao cay giong dua tren anh hudng cua den Led den su phat sinh hinh thai, smh trudng va phat tridn d thuc viit
Tuy nhien, ddi vai radi loai thvc vat c\i the se thich hop vdi mot loai den Led kliac nhau Qua ket qua nay ed the khang dinh:
Ddng Bach dan PNCT3 thich hpp nhit khi dupe nudi dudi dieu kien iinh sang den huynh quang va khdng phu hop vdi 4 loai den Led do Rang Ddng san xuat (trang 16w, trang IOw, vimg 16w, vang IOw)
Ddng keo lai BVIO thich hpp nhat khi dupe nudi dudi anh sang den Led anh sang hang 16w, cdn khi nudi cay d den Led anh sang vang IOW va 16W cay cd hien tuong thuy tinh hda.
TAI LIEU THAM KHAO 1 Doan Suy NghT, Le V5n Trpng (2006), Gido trinh Ly sinh hoc- NXB Dai hpc Hud
2, Lan Ashdow (2015), "Led lighting in Honculture,"
Reseachergate
3 S, D Gupta, A Agarwal (2017), Light emitting diodes for agriculture • smart lighting Springer Nature
4. T Kozai, M A. L Smith (1995), "Enviromental conQ-pI in plant tissue culture - genera! inU-pductipn and overview," in Automation and enviromental control in plant tissue culture, pp. 301-318
P.THanh et aWHa 14^Dec 2019|p 90-93
Influence of some LEDs produced by Rang Dong on the growth and development of shoots of Eucalyptus PNCT3 and Acacia hybrid variety BVIO in Vitro conditions
Pham Tht Hanh, Pham Thi Mai Trang, Nguyen Van Giap
Article info A b s t r a c t
Light, one of the key factors for plant growlh and development, is involved in
^^'^^^ • many physiological processes of plants, including photosynthesis. Optical process generates morphology, hglit direcUon In studying the impact of light on die
^^^^ ^ • growth and development of EucalypUis shoots PNCT3 and Acacia hybnd BVIO used different types of hghts produced by Rang Dong such as. 16w white hght 'LEDs, Led lights IOw white light, 16w yellow light Led, IOw yellow light Led Keywor s. ^^^ reference formula using 35w fluorescent lamp As a result, after 4 weeks of Led, Eucalyptus culhire, the fonnula of using 16w white light Led light of hybnd Acacia BVIO has PNCT3, Hybrid acacia
BVIO In vitro ^'^^ highest growth and development capacity; All 4 types of LEDs produced by Rang Dong used in dus study were not suitable for the growth and development of PNCT3 Eucalyptus shoots
