TCYHTH&B s6 1-2012 53
NGHIEN C d u DANH GIA T A C DUNG KHANG GdC TU DO TRONG DifeU TR! NHI§IV1 DQC BdNG CUA CH^ PHANI VB09
Chu Anh T u i n ' , Ld Ndm', N g u y i n LTnh Todn "
Vidn Bdng Oudc gia'. HQC vidn Qudn y*"
T6M TAT
Hodng lidn gidi ddc thang Id bdi thudc cd phuang. dwryc Ong dung didu tn nhidu bdnh, trong dd c6 bdng. ChOng tdi dd kd thOa vd bdo chi bdi thudc dwdi dang cao Idng 1:1 vdi tdn gpi VB09 Trong nghidn cOu ndy. chOng tdi ddnh gid tdc dung chdng oxy hod cOa chd phim VB09 thdng qua xdc djnh ho$t dd enzym superoxide dismulase (SOD), hdm luiyng manonytdialdehyde (MDA) huyit thanh cua ddng v$t thuc nghidm gdy bdng vd bdnh nhdn bj bdng ndng tinh nguydn.
Phuong phdp nghidn cOu: tht/c nghidm Idm sdng cd ddi chOng so sdnh. Kdt qud nghidn cOu cho thiy: chd phdm VB09 cd tdc dung chdng oxy hod thdng qua ca chd ldm tdng hoat tinh enzym SOD, gidm si/ hinh thdnh MDA tmng huydt thanh
SUMMARY
Thermal injury initiates systemic inflammtory reactios producing burn toxins, an inflammatory response, oxygen radicals and finally peroxidation Huanglianjiedu decoction is a old traditional prescription, application has been treated many diseases, including burns. We have all inherited and dosage drug form 1:1 highly liquid called VB09. In this study, we evaluated the effect of antioxidant of VB09 preparations through determining the activity of enzyme SOD (superoxide dismutase), serum MDA (Manonytdialdehyde) levels in plasma of experimental animals and severe burns patients volunteers. Research methods; Radomized clinical trial. Research results shovi^ed that VB09 preparations had effect antioxidant through the mechanism increases the activity of the enzyme SOD, reduced the formation of MDA.
1 . D A T V A N 0 £ nhdng v i n d l md ngdy nay dug-c cdc nhd Gdc t y do Id nhung nguydn t d , nhdm khoa hpc ddc bigt quan tdm.
nguydn t d hay phdn t d md ldp didn t d ngodi Mdt trong nhdng san p h i m taing gian cua ciing cOa chCing cd chda didn t d khdng cdp qud trinh peroxy hod lipid Id malonyl dialdehyd ddi, Trong ea the, blnh thudng gdc t y do chi (MDA). Song hdnh vdi s y tdn lgi cua gdc t y do tdn tai vdi ndng dd t h i p vd t h y c hidn mdt sd trong ca t h l Id hd thdng chdng gdc t y do chdc ndng sinh ly nhat i^nh. Khi gdc t y do sinh (antioxidant) theo ea c h l d i l u hod n h i m b i o vd ra qud mde Id nguydn nhdn Idm tdn thuang c a t h l . Nhilu nghidn c u u cho t h i y r i n g thdng c i u trdc phdn t u sinh hpc d mdng t l bdo, qua xdc dinh hogt tinh mdt sd enzym chdng oxy mdng nhdn, vdt c h i t di truyin... gdy ra n h i l u hda nhu superoxid dismutase (SOD), glutathion qud trinh bdnh ly khdc nhau nhu-: ung t h u , ldo peroxidase (GPx), trgng thdi chdng oxy hda hda, bdnh tdt, c h i t theo chuang trinh. Ody Id todn p h i n (TAS) cua ca t h l , hdm lyg-ng MDA
trong huyit thanh d l cd t h l ddnh gid Hnh trgng Phan bidn khoa hgc. TS. Ld DOc Man. stress oxy hda [1], [2], [3], [4].
54 TCYHTH&B s6 1-2012
Nghidn cdu v l bdnh ly diln biln tgi vCing tdn thuang bdng, Arturson G. (1969) dd khing djnh tgi viing cdn hogi td bdng vd vCing hogi td bdng cd sy giii phdng ra cdc gdc oxy ty do eCing cdc chit trung gian gdy vidm nhu- TNFa, IL1, eieosanoids, lactate, heat shock protein.
Khi tdn thu'ang bdng sdu vd rOng qud trinh ddp dng vidm mang tinh hd thdng. do dd ede chit trung gian vidm trdn cung vdi cdc gdc oxy ty do khdng chi dnh hudng eye bd md tdc ddng din ede tgng d xa gdy hdi chdng rdi logn chdc ndng da tgng (multiple organ dysfunction syndrome - IVIODS). VI vdy. hgn chl tdc hgi cOa gdc ty do trdn bdnh nhdn bdng r^ng Idm giam tinh trgng nghidm trpng cua bdnh ly bdng.
gdp phin ndng cao hidu qui dilu trj. Cdc nghidn cdu cho thiy. trong dilu tri bdnh nhdn bdng ndng cin phdi hpp tri lidu ea bdn hidn diing vdi ede chit chdng oxy hod ngogi sinh [2], [7]. [8], [91.
Hodng hdn giai dde thang Id bdi thudc ed phuang, gdm ede thdnh phan: hodng lidn, hodng bd, hodng cim vd chi td, diing dudi dgng sic udng, cd tdc dyng thanh nhidt thai ddc Td xa xua, bdi thude dd dupc dng dyng trong dilu tri bong, cd ldc dyng tdt. Chung tdi dd k l thda vd bdo chl bdi thudc dudi dgng cao Idng 1:1 vdi tdn gpi VB09.
Trong nghidn cdu ndy, chOng tdi ddnh gid tdc dyng khdng gdc ty do cua ehl phim VB09 thdng qua xdc d|nh hogl tinh enzym SOD, hdm lupng MDA huyit thenh trdn ddng vdt thyc nghiim vd b$nh nhdn b| bdng ndng tinh nguydn.
2. £)6l TU^NG VA PHUONG PHAP NGHIEN CLTU 2.1. Ddi tu'O'ng
- Nghidn cOu thi/c nghidm
Nghidn cdu tiln hdnh trdn thd tidu chuin, cd trgng luang td 2,5 ± 0,2 kg/con, sd lupng 60 eon (ngodi ra cdn cd 2 thd dy phdng td vong trong qud trinh thl nghidm).
Thdi gian theo ddi ddng vdt Id trudc khi gdy bdng 3 ngdy cho din kit thOc thl nghidm Id 3 tuin k l td ngdy gdy bdng
- Nghidn cOu trdn bdnh nhdn tinh nguydn Nghidn cdu dupe tiln hdnh trdn 62 b$nh nhdn bdng ndng dyp'c dilu trj tgi khoa Dilu tri tich cyc - Vidn Bdng Ld Hdu Trdc. thdi gian td thdng 6 - thdng 11/2011. Tidu chuin lya chpn bdnh nhdn nghidn cdu:
+ Bdnh nhdn cd didn tich bdng chung trdn 30% DTCT hodc didn bdng sdu trdn 20% DTCT.
+ Vdo vidn trong 3 ngdy diu sau bdng.
+ Khdng mdc cdc bdnh mdn tinh ndng kdm theo.
+ Ty nguydn tham gia nghidn cdu.
2.2. V^t li$u v i hda chit nghidn cdu - Thudc nghidn ci>u: Id cao long VB09, do Trung tdm nghidn cdu dng dyng thudc - Hpc vidn Qudn y sin xuit. Thdnh phin cao Idng gdm cdc vj thudc hodng lidn, hodng bd, hodng cim vd chi tu. Thude dgt tidu chuin ca sd cOa mdt thudc udng nhu* v l dd an todn, vd khuin, pH trung tinh, ty Id hogt chit chlnh theo ydu c i u . .
Thudc diing cho ca dpi nghidn cdu dupc sin xuit cimg Id, cd sd lu'ang vua du, thudc trong hgn sd dyng vd dim bao chit luang
- Hod chit: Kit xdc djnh hogt tinh SOD cua hdng BIOVISION (SOD Elisa kit - code:
K335-100), kit djnh lupng MDA cua hdng Cusabio Biotech (Human Malondialdehyde (MDA) ELISA kit. catalog NO: CSB-E08557h).
2.3. Phu'O'ng phip nghiin cdu
Thiit kd nghien cdu; Nghidn cuu tiln cdu. thyc nghidm Idm sdng cd ddi chdng so sdnh.
- Nghidn cdu trdn thd th{K nghidm
Thd khde mgnh dug-e gdy bdng hai bdn
sdng lung ciing di$n tich 30% dd III ndng, sdu
TCYHTH&B s6 1-2012 55
theo phuang phdp cua Podicalo J.J (1955), Haldovec. J.U (1961). Sau dd d u p c ehia n g i u nhidn thdnh 02 nhdm (mdi nhdm 60 eon).
+ Nhdm 1 (nhdm Nghidn cOu). Du'pc d i l u tri tgi chd vet bong bdng kem Silvirin 1 % (SSD1%) vd udng 5ml thudc cao ldng VB09 x 3 lin/ngdy
+ Nhdm 2 (nhdm ChOng): Du'pc d i l u trj tgi chd v l t bdng bdng kem Silvirin 1 % vd udng 5ml thudc n u d c mudi smh ly (NMSL) x 3 lan/ngdy.
Xdt nghidm xdc dinh hogt tinh SOD, hdm lupng MDA d u p e t i l n hdnh d 4 thdi d i l m . No (ngay trude gdy bdng). N l (ngdy t h d 3 sau bdng). N2 (ngdy thd 10 sau bdng), N3 (ngdy thd 17 sau bdng).
- Nghidn cuu trdn bdnh nhdn tinh nguydn Bdnh nhdn (BN) tinh nguydn nghidn cdu d u p c chia n g i u nhidn thdnh 2 nhdm, tuang duang nhau v l Ida ludi. didn bdng chung vd bong sdu:
+ Nhdm nghidn cwu. gdm 32 bdnh nhdn.
BN dupe d i l u tn theo phdc dd chuan dang dp dyng tgi Vidn Bdng Ld Huu Trac vd t d ngdy thd 3 sau bdng d u p c cho udng VB09 vdi l i i u 2 - 2,5 mi/kg cdn ndng/ngdy, chia Idm 3 lan sang, tru'a, c h i l u
+ Nhdm ChOng- gdm 30 b$nh nhdn BN du-pc d i l u trj theo phdc dd c h u i n dang dupc dp dyng tgi Vidn Bdng Ld Hdu Trdc.
Xdt nghidm xdc djnh hogt tinh SOD, hdm lupng MDA d u p c t i l n hdnh d 3 thdi d i l m : N l (ngdy thd 3 sau bdng). N2 (ngdy thd 10 sau bdng), N3 (ngdy thd 17 sau bdng)
- Xdc djnh hogt tinh SOD theo phuang phdp cua Sun Y vd cdng s y (1988) [11] 0|nh lupng MDA theo phuang phdp cua Tanaka M, v d e d n g s y (1994) [3],
2.4. Xd ly sd li^u
Theo phuang phdp thdng kd y hpc, s d dyng p h i n m i m SPSS 15. vd STAVIEW 5.1.
Trong qud trinh x d ly sd lidu chung tdi s d dyng cdc thudt todn sau:
- So sdnh 2 sd trung binh cua 2 nhdm ddc ldp diing thudt todn T test
- K i l m d|nh khi blnh phuang {%' tesl).
3. K^T QUA NGHIEN C i r u
3.1. K i t qua x l t n g h i f m tren thd t h i ^ nghidm
Bang 3.1: B i l n ddi ho^t tinh SOD trong mdu cua cac nh6m thd tgi cdc than d i l m nghi&n cuu Nh6m
Nghien ci>u
Doi chi/ng
SOD 1%) X SD X SD P
No(1) 68.545 9,236228
66,545 9,236228
N1(2) 93,81111 10,83587 7813333 9,267026 p<0,01
N2(3) 92,80778 16,13766 79.67667 9,729137 p<0,01
N3(4) 91,35778 13,83153 84,32222 7,715288 p < 0.05
Nh$n xdt: Hogt dd SOD mdu tdng cao rd r^t sau bdng ngdy t h d 3, t h d 10 vd 17 sau bdng ndng so thdi d i l m t r y d c khi gdy bong
thd, CCing m$l thdi diem sau bdng thi hogt tinh SOD d nhdm dupe udng cao Idng VB09 cao han so vdi nhdm udng NMSL, khdc bidt c6 y nghTa thdng kd vdi p<0,05 vd p<0,01.
56 TCYHTH&B s6 1 - 2012
100 90 80 70 60
1 "
30 20 10 0
Ng«vO(l) Ngay 3 (2) Ngiy 10 (3)
,
Ngiy 17(41
- • - C « > V B 0 9 Hi-NMSL
Bilu dd 1: Biln ddi ho^t tinh SOD (%) trong mdu cua cdc nhdm thd tgi cdc thdi dilm nghidn cdu, (X ± SE)
Bdng 3.2: Biln ddi ndng dd MDA trong mdu cua cdc nhdm thd tfl cdc thdi dilm nghidn cdu (n = 30) Nham
Nghign ci>u
Doi chi/ng
MDA (|ig/ml) X SD
X SD P
No(1) 4.9470619
1.218945 4.9470619 1.218945
N1{2) 5.58752 1.41563 7.6609464 1.4525971 0.0004692
N2(3) 5.9403016
1.935235 6.1784711 1.5946224 0,00(7632
N3(4) 5,8867395
1.983643 7,2210088 2,882231 0,0215583
P Nhom NC Nh6m chjng
p1.2 0,195 0,00006
pi-3 0,0993 0,0246
p1-»
0,1693 0,0086
p2J 0,0128 0,0127
p2-»
0,9678 0,2305
p3-*
0,0145 0,2305
Nhdn xdt Hdm lupng MDA trong huyit dilm sau bdng thl hdm lyp-ng MDA d nhdm tuang tho bj bong ndng tdng cao rd r$t vdo cdc 6w(?c udng cao ldng VB09 thip han so vdi ngdy thd 3, ttid 10 vd 17 sau bdng so thdi nhdm udng NMSL, khdc bi$t cd y nghTa thdng dilm trudc khi gdy bdng thd. Ciing mdt thdi kd vdi p < 0,05 vd p < 0,01.
TCYHTH&B s6 1 - 2012 57
i-VB09 l-NMSL
B i l u dd 2: B i l n ddi ndng dd MDA trong mdu cua cdc nhdm thd t^l cdc thiri d i l m nghidn cdu 3.2. Kit qui xet nghidm trdn b^nh nhdn bdng n|ing
Bdng 3.3. B i l n ddi ho^t tinh cua SOD trong mdu b$nh nhdn bdng n^ng d cdc tiidi d i l m nghifin cdu Nh6iii
NghiSn ciju
Doi cht>ng
SOD (%) X SD X SD P
N1 85,425 (n = 25)
9,134 86,136 (n = 22)
10,457 p > 0,05
N2 92,189 (n = 17) 12,367 77,239 (n = 17) 11,247 p<0,01
N3 133,967 (n = 12) 11,458 89,753 (0 = 12)
9,823 p < 0,01
P p1-2 <0,05 pi •3< 0,01 p2-3 <0,01 p1-2 <0,05 p1-3 >0,05 p2-3 <0,05
Nh$n xdt: Hogt dd SOD mdu tdng cao rd rdt vdo ede thdi dilm ngdy thd 3. thd 10 vd 17 sau bdng ndng Cimg mdt thdi dilm sau bdng thi hogt tfnh SOD d nhdm nghidn cdu cao han rd rdt so vdi nhdm chdng, khde bidt cd y nghTa thdng kd vdi p<0,01.
Bang 3.4. B i l n ddi ndng dd MDA trong mdu b^nh nhan bdng n$ng d cdc thdi d i l m nghidn cdu Nhom
Nghign ci>u
Doi chi>ng
MDA (pg/ml) X SD X SD P
Nl 6,552 (n = 25)
1,346 6,136 (n = 22)
1,267 p > 0,05
N2 5,347 (n = 17)
2,127 5,964 (n = 17)
11,247 p < 0.01
N3 5.095 (n = 12)
1.433 5.643 (n = 12)
9,823 p < 0,01
P p1-2 <0,01 p1-3<0,01 p2-3 <0,05 p1-2 > 0.05 p1-3 < 0.05 p2-3 < 0.05
Nh0nx6t:
- HSm iirffng MDA tSng cao nhSt ir ng4y thir 3 sau bdng 6- ca hai nh6m BN
- d cung thiSi diem N2 (ngSy thir 10), N3
(ngdy thi> 17) sau bdng, hSm lu'p'ng MDA ir nh6m
BN NghiSn cipu thip hon so vdi nh6m Chi>ng,
khSc bist c6 J nghTa th6ng kS vdi p < 0,01.
58 TCYHTH&B so 1 - 2012
4. BAN LUAN
Td ndm 1984. Culler R.G dd xdc djnh vai trd bdnh ly cua gdc ty do (free radicals) trong sinh hpc. Din ndm 1986, Harman D. ndu mdi lidn quan giua cdc gdc ty do vdi ngudn gdc vd qud trinh tiln tnen cua sinh vdt, qud trinh ldo hod vd qud trinh b$nh iy Cdc gdc ty do gdy oxy hod idp md cua mdng t l bdo vdi malonyl dialdehyd (MDA) Id sdn phlm cudi cCing cua qud trinh ndy, Id nhdng nguydn nhdn gdy tdn hgi mdng t l bdo, nhdn t l bdo rit mgnh [1]
Ddi vdi bdnh ly bdng, cdc tdc gid Wooliscroff F.O vd cdng sy (1990) [12] dd Chung minh mdt edeh hiin nhidn v i sy sinh sin ra ede gdc oxy ty do d ngudi bi bdng khi didn tich tdn thuang bdng trdn 20% DTCT trd Idn, Cdc xdt nghidm eho thiy:
+ Ndng dd cdc lipid peroxides d huyit tuang, da, nhu md phdi deu tdng cao.
+ Cd bilu hidn thodi rd cua ATP. Cdc gdc oxy ty do ndy trd thdnh cdc trung gian gdy hgi cho nhu md ede tgng, ddc bidt cdc t l bdo cua phi nang phdi, t l bdo nhu md thdn, gan
Theo Edalmas vd cdng sy (2001), trong bong sy giai phdng ra cdc gdc oxy ty do thl hidn mdt trgng thdi stress oxy hod (oxydalive stress) d nhdng b$nh nhdn bdng ndng cd di$n tich bdng td 30% DTCT trd Idn [2]. Nghidn cdu eua cdc tdc gia nhu Nguyen TT, Cox CS vd cdng sy (1993) [8], Kumar R. vd cdng sy (1995) [6], Babik F. vd cdng sy (2001) [2], ...
deu eho thiy d nhdng blnh nhdn bdng ndng ndng dd cdc chit oxy cd tinh ndng phan dng smh hpc, ndng dd MDA tdng vd ndng dd men SOD, catalase diu giim trong nhdng ngdy diu sau bdng vd phgc hdi td ngdy thd 15.
Demling R.H vd cdng sy (1995), dd nhdn thiy c6 mdi lidn hd gida ndng dd gdc oxy ty do tdng cao trong mdu chudt gdy bdng thyc nghidm vdi ty Id td vong [5].
Cd mdi lidn hd chdt chd gida enzym chdng oxy hod (SOD) vdi sin phim trung gian eua qud trinh peroxy hod lipide. Cetinkale vd cdng sy (1997) dd tiln hdnh gdy bdng thyc nghidm cho thd, vdi di$n tieh bdng 30% DTCT, sau dd tiln hdnh xdt nghi$m mdu thd trong 3 ngdy diu
sau bdng, kit qui eho thiy ndng dd MDA Id 1,75 ± 0,59 nmol/mi (so vdi nhdm chdng khdng bdng Id 1,11 ± 0,37 nmol/ml, khdc bj$t vdi p < 0,02). cdn ning dd SOD Id 15,85 ± 3,39 p/ml (nhdm chdng khdng bdng: 30.97 ± 4,19 p/ml, p < 0,0001) [4] Mai Mgnh Tuin vi cdng sy (2007) nghidn edu trdn b$nh nhi bfing ndng, cho thiy tinh trgng peroxy hod lipid xdy ra ngay sau khi bj bdng. thl hidn d ndng df MDA cao nhit trdn tit c i cdc b$nh nhdn tif ngdy thd nhit din ngdy thd 3 sau bdng (2,31 0,28 nmol/1), giim xudng d nhdng ngdy thd 7 vd 14 sau bdng (p < 0,05), Kit qui nghidn cihi cQng cho thiy ndng dd men SOD giim mgnii nhit ngdy 7 sau bdng. din ngdy thd 14 vin ehua hdi phgc. Ndng dd men SOD giim chdng td phin dng oxy hod rit mgnh, vl vdy lupng enzym chdng oxy hod dd dupc huy ddng vd si) dung nhilu tuang dng [3].
Trong dilu tri cdc bdnh nhdn bdng ndng.
ein phii diing phdi hpp cdc chit chdng oxy hod ngogi sinh. Malsuda T. vd cdng sy (1993) dd dimg vitamin C lilu cao trong nhdng QHJ dau sau bong thiy cd hi$u qud tdt [7]. Mdt si ldc gia cdn sd dgng glutathion dudi dgng khi dung cd tdc dgng bio vd nhu md phdi vd ngdn sy tdng sinh hydrogen peroxide do qud trinh tdng oxy hod do cdc gdc oxy ty do d t l bdo nhu md cdc phi nang. Sd tdc gii khdc d l ngiii sd dgng vitamin C, p - caroten, selenium lectin d l tdng cudng cho qud trinh ty bio vd ciia co thl chdng cdc gdc oxy ty do trong trj li$u ch&a blnh bdng [2] Qun Zang (2008) dd cdng bd kit qua thudn iai trong dilu tri b$nh nhdn bdng ichi kit hpp vdi li$u phdp chdng oxy hod 19],
Hodng lidn giai ddc thang td xa xua (IS
dupc bilt din cd tdc dgng thanh nhi$t thai
ddc Nghidn cdu cua cdc tdc gii WANG Li-jin,
Xu Quiang (2000) [13], Zhao Bao-sheng vd CS
(2009) [14],... diu khdng djnh Hodng lidn gi^i
ddc thang cd tdc dgng chdng vidm, khdng
khuin khd tdt, dya trdn ca chl ldm giam s\f
hlnh thdnh cdc cytokin vidm, cdc gdc NO, Theo
Song Jue, LU Tong vd CS (2010), Hodng lifin
giii ddc thang cd tdc dgng chdng oxy hod
mgnh trdn thyc nghidm bdng ndng, phg thuOc
vdo lilu lup^ng vd thdi gian sd dgng [10].
TCYHTH&B s6 1-2012 59
Trong nghidn cuu ndy, d l ddnh gid tdc dgng cua ehl phim din khi ndng khdng gdc ty do, chiing tdi sd dgng phuang phdp xdc djnh hogt tinh cua enzym SOD (%), chu khdng xdc djnh ndng dd cua enzym nhu cdc nghidn edu eua cdc tdc gia khdc. Kit qui nghidn cdu cOa Chung tdi cOng eho thiy, trdn thd thyc nghidm gdy bdng, hogt tinh SOD mdu tdng cao rd rdt sau bdng ngdy thd 3, thd 10 vd 17 sau bdng ndng so thdi dilm trudc khi gdy bdng thd. Trdn bdnh nhdn bdng ndng, hogt tinh SOD cung tdng cao kdo ddi sau td ngdy thd 3 din ngdy 17 sau bdng. Kit qui ndy cho thiy m$t phan dng oxy hod rit mgnh dd xay ra d thd thyc nghidm gdy bdng ndng vd bdnh nhdn nghidn cdu, Idm tdng hogl tinh men SOD.
Nghidn cdu cung cho thiy, cimg mdt thdi dilm sau bdng thl hogt tinh SOD d nhdm dupc udng cao long VB09 cao han so vdi nhdm chdng, khdc bi|t cd y nghTa Ihdng kd vdi p<0,05 vd p<0,01, Oilu ndy chdng td, chl phim VB09 cd tdc dgng kich thich ldm tdng hogt tinh, tdng kha ndng chdng oxy hod cua enzym SOD.
Bdi vdi xdt nghidm MDA, kit qua nghidn cdu cQng cho thiy hdm lup^ng MDA tdng cao d cdc thdi diem ngdy thd 3, thd 10 vd thd 17 sau bdng. Cimg mdt thdi diem sau bdng thl hdm lypng MDA d nhdm dupc udng cao long VB09 ludn thip han so vdi nhdm chdng, khde bidt cd y nghia thdng kd vdi p<0,05 vd p<0,01 Oilu ndy chdng td, ehl phim VB09 cd tdc dyng Idm giim qud trinh hlnh thdnh cdc gdc oxy ty do, giam qud trinh oxy hod chit bdo (sin phim cudi cung Id MDA), cd tdc dgng bio vd co thl chdng oxy hod.
5. KgT LUAN
Cao long VB09 diing dudng udng cd tdc dgng chdng oxy hod trong dilu trj bdng ndng (ddng vdt gdy bdng thyc nghidm vd b|nh nhdn bdng) thdng qua qua ca c h l :
- Ldm tdng hogt tinh cOa men SOD:
CDng mdt thdi dilm sau bdng thl hogt tinh SOD d nhdm du^c udng cao ldng VB09 cao han so vdi nhdm chdng, khdc bidt cd y nghTa thdng kd vdi p<0,05 vd p<0,01.
- Ldm giam hlnh thdnh gdc ty do, giim qud trinh oxy hod chit bdo tgo thdnh MDA:
CCing mdt thdi dilm sau bdng thl hdm lupng MDA d nhdm dupc udng cao ldng VB09 ludn thip han so vdi nhdm chdng. khdc bidt cd y nghTa thdng kd vdi p<0,05 vd p<0,01, TAI L1$U THAM KHAO
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