• Nghien CLFU - Ky thuat
studies", Anf/cancer Res. 23(1 A), 363-98.
3. Chaudhary G. (2011), "Inhibition of dimethylebenz (a) anthracene (DMBA)/croton oil Induced skin tumorigenesis in Swiss albino mice by Aloe vera treatment". International Journal of Biological & Medical Research, 2(3), 671-678.
4. Huang M. T, Newmark H. L., Frenkel K. J. (1997),
"Inhibitory effects of curcumin on tumorigenesis in mice", CellBiochem. SuppI. 27, 26-34.
5. Hunter J., Savin J., Dahl M. (2002), C//n/ca/
Dermatology Biackwell Science Ltd. Co., 253-282 6. Huynh NT Morille M, Bejaud J, ef ai (2011).
'Treatment of 9L gliosarcoma in rats by fen-ociphenol- loaded lipid nanocapsules based on a passive targeting strategy via the EPR effecf, Pharm. Res., 28 (12), 3189-98.
7. Limtrakul P.(2001), Curcumin inhibits carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression, and protein kinase c activity in mouse skin, BMC Cancer, 1:7.
8. Limtrakul P., Anuchapreeda S., Lipigorngoson S, ef al. (2001), "Inhibition of carcinogen induced c-Ha- ras and c-fos proto-oncogenes expression by dietary
{Ngay nhan bai: 14/03/2016
curcumin", BMC Cancer, 1,1.
9. Manoharan 8., Selvan M. V. (2012),
"Chemopreventive potential of geraniol In 7,12-dlmethylbenz(a) anthracene (DMBA) Induced skin carcinogenesis in Swiss albino mice", J. of Environmental Biology 32, 255-260.
10. Quintanllla M., Brown K., Ramsden M., ef al.
(1986), "Carcinogen specific mutation and amplification of Ha-ras during mouse skin carcinogenesis". Nature 322, p. 78-80.
11. Sonavane K, Phillips J, Ekshyyan O, etal (2012),
"Topical curcumin - based cream is equivalent to dietary curcumin in a skin cancer model" J. of Skin Cancer, 2012,Article ID 147863.
12. Wilken R., Veena M. S., Wang M. B., ef a/.
(2011). "Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma". Molecular Cancer, 10, 12.
13. Yuspa S. H., Hennings H., Saffiotti U. (1976).
"Cutaneous chemical carcinogenesis: past, present and future". The J. of Investigative Dermatology, 67,199-208
• Ngay duyet dang: 25/04/2016)
Tac dung khang khuan, chong viem
cua cay tam phong {Cardiospermum halicacabum L.)
/ Du-oTig Thi Ly Hu-oTig'', Nguyin Thanh Thao^ Tran Van On^
' 'Khoa YDuac, Dai hpc Quoc gia Hd Npi
'Truong Dai hpc Duac Hd Npi 'E- mail: [email protected]Summary
Antimicrobial activity of Cardiospennum halicacabum L against Escherichia coli and Samoneila sp. was evaluated by the diffusion method on agar plates. No sterile circles were obsen/ed with either aqueous and alcohol extracts from the plant in experimental bacteria incubation. Acute anti-inflammatory action was tested on earrageenan-induced paw edema in rats and peritonitis exudation, subacute inflammatory property through weight gain of granuloma caused by subcutaneous implantation of amiante, and the thymus weights after using the test agents. Both aqueous and alcohol extracts from Cardiospermum halicacabum L. exhibited acute anti-inflammatory action. Only the aqueous extracts decreased the weight of granuloma. Neither aqueous nor alcohol extraction affected the weight of thymus after 3 days' administeration.
Keywords: Cardiospermum halicacabum L, antibacterial, anti-inflammation.
D a t v a n d e
T i m phdng {Cardiospermum halicacabum L.) la met cay than thao, s i n g lau nam, phan bd chu y l u d vitng nhiet ddi va can nhiet ddi ehau My, chau Phi, va chau A. Cay d u g e dung kha phd b i i n d l lam thude d l l u trj cac benh n h u viem da khdp dang t h i p , eczema, viem da, viem d u d n g t i l t nieu, viem than, phu t h u n g . . . P " ^ i " ! . Ngoai ra, eay cdn ed tae dung khang histamin, khang khuan, chdng
ung t h u da day ^^•^. Cae nghien cu-u v l thanh p h i n hda hpe eho t h i y toan cay t i m phdng ed chira saponin, alcaloid, flavenpid, prpanthocyanidin, apigenin va phytosterol P"'^. Phan dean steroid, cao edn p h i n tren mat d i t eiia eay t i m phdng cd hoat tinh chdng viem tren thir nghiem gay phu chan chupt edng bang carragenan. Cao cdn la cd tac dung giam dau, chcng viem trong thir nghiem gay u hat thge nghiem. C o c h i c h i n g viem cua eay
30
TAP CHI DUrOC HOC - 5/2016 (SO 481 NAM 56)
Nghien CCPU - Ky thuat
t i m phdng dugc chipng minh la ed lien quan d i n COX PI. Ngoai ra, djeh chilt t i m phdng edn lam giam TNFa va NO '^l Ben canh tac dung chdng viem, tam phdng cdn dugc bilt din vdi tae dung khang khuin w, dllu trj eczema P"', ha huylt ap, Igi tieu I'l, giam dudng huylt PI, diet l u trung san'"'...
O Viet Nam, t i m phdng dugc bilt din chii y l u vdi tae dung chu-a eae benh ngeai da. Cae nghien ciPU v l thanh phan hda hpe, tac dung sinh hpc, cung nhu cac Igi ich khae eua cay tim phdng d i i vdi sire khde con ngudi edn chua duge bilt rd. Mue tieu eua d l tai la danh gia tae dung khang khuan va ehdng viem ciia djch chilt toan phin eay t i m phdng d Viet Nam.
Nguyen lieu va phu'O'ng phap nghien ciru Nguyen lieu
Nguyen lieu la toan bd phin cay tren mat dit ciia cay t i m phdng (cay bdp bep - tiing Mu-dng), duge thu hai vae thang 7/2015 tai xa Long Son, huyen Kim Bdi, tinh Hda Binh. Miu duge sly khd d nhiet dp 50°C va bae quan trong tiii nilon. Mau tieu ban dugc luu tai phdng tieu ban Trudng Dai hpc Du-gc Ha Ndi (HNIP), so hieu mau HNIP/15362/08.
D l nghien cuu tac dung duge ly, sir dung djch chilt nudc va djch ehilt edn eiia toan cay t i m phdng, cu the nhu sau:
Djeh chilt nude: nguyen lieu khd duge tan nhd, sic 3 Iln bang nu-de, gdp nudc sac lai, Ipc qua bdng, cd din cae Idng 1:1.
- Djch chilt cdn: nguyen lieu khd duge tan nhd, chilt bing dung mdi ethanol 70°. Dich ehilt dugc Ipe qua bdng, d t thu hdi dung mdi va cd each thiiy din eae Idng 1:1.
Ool tu-gng nghien CIFU
Cae chung vi khuan Escherichia coli ATCC 29522 va Samoneila sp. ATCC 14028 do phdng vi sinh, Vien Kilm nghiem Trung uong eung clp.
- Chupt edng trang, khde manh, ca 2 gidng, can nang trung binh 160 ± 20 gam, do Hpc vien Quan Y eung clp. Rieng ddi vdi md hinh chdng viem thdng qua tuyin iec, su dung ehudt edng dgc cdn non, trpng lugng trung binh 100 ± 20 gam.
Phu-cng phap nghien CLFU
Tac dung khang khuan+ Nguyen tic: Heat chit ed treng ehl phim thir khuech tan vae mdi tru-dng dinh du-dng ed vi sinh vat chi thj. Sau thdi gian (24 gid) ii ehl pham thir vdi mdi trudng dinh dudng ed chira vi sinh vat chi thj, do du-dng kinh vdng vd khuan d l danh gia kha nang ipc ehl vi sinh vat ciia miu nghien ciru.
+ Cach tiin hanh: Chuin bi mdi trudng
dinh du-ong thach thudng. Hap tiet trung d 121°C trong 15 phut Khi nhiet dp mdi tnj-dng khoang 45-50°C, trdn vi sinh vat ehi thi vao mdi tru-dng va lie deu (vi sinh vat ehi thj trudc khi thir dugc lam thanh eae nhu djch cd kheang 10^-10' t l bae trong 1 ml, va trdn vao mdi trudng theo ty le 1%)).
Dd mdi trudng dinh dudng ed chira vi sinh vat ehi thj vao eae hop petri da duge hip tiet trung d 121°C trong 15 phut (25 ml/hdp). Sau dd eho eae hop petri vao trong budng nudi d y . Sau khi thach ddng, tien hanh due Id (6 gilng/dTa, dudng kinh 7 mm). Nhd 0,2 ml miu thir vao cae gilng thach. Be yen 15 phut cho miu thir khuylch tan. Cho vao tu I m nhiet dp 28-30°C trong 24 gid. Sau 24 gid, do dudng kinh vung LPC ehl d l danh gia kha nang ipc ehl vi sinh vat eiia miu nghien ciru. Nlu dudng kinh vdng vd khuin > 15 mm, heat chit cd treng chi pham thu- cd tinh khang khuin.
Tac dung chong viem
+ Md hinh gay phii chan chuot bing cauageenan:
theo md hinh eiia Winter*^'. Chupt duge chia nglu nhien thanh 4 Id nghien ciru (Id 1 udng NaCI 0,9%, Id 2 udng Indomethacin 5 mg/kg. Id 3 udng djch ehilt t i m phdng nudc 10 g/kg va Id 4 udng djch chilt tam phdng edn 10 g/kg). Tit ea ehudt dugc udng chit thir vao thdi dilm 24 gid va 60 phut trudc khi gay viem bang earrageenin 0,5% (pha trong NaCI 0,9%) tiem vao gan ban chan ehudt.
Trudc khi gay viem, chupt dugc de the tieh ban chan ehudt binh thudng (V^), sau khi gay viem 1 gid, 3 gid, 5 gid, 24 gid ehudt duge do lai the tich ban chan chupt (duge eae gia trj V,, V3, V^, V^^).
Kit qua ehdng viem dugc t h i hien bang muc dp phu Chan ehudt (DV%) va ty le phan tram uc ehl phu (1%).
+ Md hinh gay tran dich mang bung: Chupt dugc ehia thanh eae Id gidng nhu tren, dugc udng thude lien tuc trcng 3 ngay Ngay thir 3, mdt gid sau khi udng thude thip, ehudt duge tiem mang bung dung djch earrageenin 0,005 g + formaldehyd 1,4 ml + nude d t vua dii 100 ml vdi the tieh tiem 2 ml/
ehupt, Sau khi tiem 24 gid, md khoang mang bung d l hut djch va dim sd lugng t l bao mau trong djeh tilt. Tae dung chdng xuit tilt duge danh gia qua t h i tieh djch ri viem va sd lugng bach elu trong djeh ri viem.
+ Md hinh gay viem man bing amiant: Sir dung phuong phap gay u hat thge nghiem eiia Ducrot, Jelou va cdng sg ra. Chudt dugc chia Id nhu- tren va udng thudc lien tue trong 5 ngay liin (vac 8.30 sang). Trudc khi che udng thude, tat ea eae chupt dugc d y amiant vdi khdi lu-gng 30 ± 0,1 mg (da tiet trung d 160°C trong 2 gid) vao dudi da lu-ng ehudt.
TAP CHI DU'OC HOC - 5/2016 (SO 481 NAM 56)
31
Nghien CCFU - Ky thuat
Chilu ngay thu 5, sau lilu uong thudc culi eung, giet ehupt, bdc tach khdi u, dat vao tii sly d nhiet dp 56°C, sly treng 18 gid, sau dd can trpng lugng khii u. Tac dung chdng viem dugc danh gia bing ty le phin tram giam trpng lugng khdi u giu-a Id thir vdi Id chirng.
+ Tac dung chdng viem thdng qua tuyin ire: Chupt duge chia Id va udng thude tuong tu nhu tren, rieng Id 2 thay indomethacin bang prednisolon 6 mg/kg treng 3 ngay (vao 8.30 sang).
Hinh 1: Tac dtjng khang khuin eiia djch ehiit tim phdng nuac ddi vdi: A) E. coli; B) Salmonella sp.
Nhan xet Kit qua eho thiy djeh chilt nude t i m phdng cd tac dung kim khuin ylu, tuy nhien khdng quan sat thiy vdng vd khuin d ca djch chilt nude va djch chilt con t i m phdng.
60.00 50.00 40.00 3 0 00 20.00 10.00 0 00
- -
.it^^^,^^""''^ •*-_.
Igic^ 3 gid- 5 gio-
— • — N a d 0,9% — • — Indomethacin Smji/kg
— • — T a m p h o n g n u - C T C 1 0 g / k g — • — T S m phdng c&n l O g / k g
Chilu ngay thu- 3, gilt chupt, bde tach tuyin ire dem can ngay So sanh trpng lu-gng tuyin ire giua Id thip va Id chipng.
Ket qua nghien cu'u
Tac dung khang khuan cua djch chiet tam phong
Sip dung 2 chiing vi khuin la £ coli va Samoneila sp. de do boat tinh khang khuin ciia dieh chief eay t i m phdng. Kit qua the hien d hinh 1 va hinh 2
Hinh 2: Tac dung khang khuin ciia dieh chiit tam phong edn doi vai: A) E. coli; B) Salmonella sp.
Tac dung chong viem cap tren mo hinh gay phil chan chuot bang carrageenan
Kit qua t h i hien qua hinh 3 va bang 1
Hinh 3: Mirc do phii chan ehudt (AV%) theo thai gian
(:p< 0,05 so vai chirng: ":p < 0,01 so vdi chung;'": p < 0,001 so vdi chimg)
Banc
Lo Thuoc dung 1 NaCiO,9%
2 Indomethacin 5 mg/kg 3 T§m phong nu-dc 10 g/kg 4 Tim phong c6n 10 g/kg
1: Tyle phin So chugt (n) -
10 10 10 10
fram iie che
Sau 1 gia 0 57,96 24,29 1,76
phil (1%) ciia cac Id chudt thuc nghiem Ty le phan tram iec che phil (1%) Sau 3 gia
0 55,61 28,30 31,06
Sau 5 gia 0 40,62 35,20 29,18
Sau 24 gia 0 30,80 16,68 8,14
Nhan xet: Indomethacin lam giam muc dp phu
chan chudt rd thep thdi gian, ea dich ehilt nu-dc va djch chilt edn d i u cd tae dung ire ehl phu chan chudt rd d cac thdi diem sau khi gay viem 3 gid va 5 gid, ngoai ra djeh chiet nudc t i m phdng cdn lam giam viem d ngay thdi diem 1 gid sau khi
gay viem. Khdng cd sg khae biet v l tac dung ehdng viem ciia indomethacin 5 mg/kg, djeh chiet nu-dc va djch chiet cdn eay t i m phdng 10 g/kg d cac thdi dilm sau gay viem 3 gid va 5 gid.
Tac dung chong viem cap tren mo hinh gay tran dich mang bung
32 TAP CHi DU'OC HOC - 5/2016 (SO 481 NAM 56)
Nghien CCFU - Ky thuat
Bang 2: Thi tich dich ri viem va sd lugng bach cau trong dich ri viem
" S T Lo Thuoc diing chuot
-(n)-
Thi tich djch So luong ri viem (ml) bach ciu (G/L) 1 NaCI 0,9% 3,53 ±0,03 8,65 ±0,36
Indomethacin 5 mg/kg
4,33 ±0,09 P,.r0.3
8,61 ± 0,92 P,.2=0.6 , Tam phong
nu-adOg/kg
10
4,08 ±0,01 P,-3=0.44,46 ± 0,34 P,-3 = 0,002 . Tam phong con . .
10 g/kg
4,30 ±0,29 '•''t"^' p =04 PM = 0-0001 P» "'^ p, =0,52 Nhan xet: Indomethacin, dieh chiet n u d e va djch c h i l t edn eay t i m phdng khdng lam thay doi the tich djch ri viem. Sd lugng bach d u giam mdt each dang ke d Id ucng tam phdng n u d e va t i m phdng edn, tuy nhien khdng thay doi d Id udng indomethacin vdi l i l u 5 mg/kg. Khdng ed s g khac biet v l sd lugng bach d u giu-a 2 Id udng t i m phdng n u d c va t i m phdng cdn.
Tac dung chdng viem man bang amiant Bang 3: Trpng lugng u hat va tyle ire ehi u hat
tren eae Id ehudt thue nghiem
Lo Thuoc dung
1 NaCI 0,9%
. indomethacin 5 mq/kq . Tam phong nu-OT
10 g/kg . TSm phdng c6n
10 g/kg
So chuot
(n) 10 10 10 10
Trpng /uong u fiat frung binh
(mg) 188,60 + 0,02 118,70 + 0,01
83,14 + 0,01
135,23 + 0,01 Tyleuv cheu hat
(%)'
0 37,08
55,99
28,28 P
P „ = 0 , 0 2 3 P,,= 0,001 p,, =0,013 P „ = 0 . 1 0
Nhan xet Ca indomethacin va djch chiet nude t i m phdng d i u ed tae dung chong viem man thdng qua giam trpng lugng u hat thge nghiem.
Ty le ire ehe u hat eiia djch chilt nude t i m phdng edn manh hon so vdi indpmethaein l i l u 5 mg/kg (p < 0,05). Dich chiet edn t i m phdng chua t h i hien tac dung e h i n g viem thdng qua md hinh nay.
Tac dung chdng viem thong qua giam trong liFgng tuyen li-c
Bang 4: Trpng lugng tuyin ire eiia cac Id chudt thuc nghiem
Lo Thuocdiing
1 NaCI 0,9%
2 Prednisolon 6 mg/kg TSiTi phong nifdc lOg/kg Tim phong c6n lOq/kq
So cfiuof
I")
10 10 10 10
Trpng fippTjg fuyen i k trung binh (mg)
0,23 + 0,01 0,15 + 0,01 0,22 + 0,01
0,22 + 0,01
Tyle giam trong luong tuyen (K so
voi Id chimq 1%)
34.56 4,54
4,93
p so ra chimg
0,013 0,79 0,74
TAP CHi DlTgfC HOC - 5/2016 (SO 481 NAM 56)
Nhan xet Chi cd prednisolon 6 mg/kg lam giarn trpng l u g n g t u y i n u-e se v d i Id udng nu-de mudi sinh ly met each ed y nghTa thdng ke. Ca djeh e h i l t n u d e va djeh c h i l t edn t i m phdng d i u ehua lam thay doi trpng lugng t u y i n ire so vol Id chirng
Ban luan
Ve tac dung khang khuan: k i t qua nghien ciru d hinh 1 va hinh 2 eho thay djeh e h i l t n u d c va edn t i m phdng khdng t h i hien heat tinh khang khuan rd tren eae ehiing E. coti va Samoneila sp. Nghien CLPU ciia Jigna Parekh eung cho t h i y djch e h i l t n u d e va edn cay tam phdng khdng cd tae dung ddi vdi E. coli '•*'. Trong khi cac nghien ciru khac eho t h i y alkaloid tu- qua t i m phdng ed tae dung ipc e h l £ . coli, Klebsiella pneumonia. Shigella boydii, va Salmonella typhimurium i^*'. N h u v a y r i t ed the vdi each c h i l t khae nhau, d cac phan doan khac nhau, djch c h i l t t i m phdng se che nhung tac dung khac nhau tren eae chiing vi k h u i n .
vi tac dung chong viem: K i t qua nghien CLPU ehe t h i y ea djch chiet n u d c va dich c h i l t edn t i m phdng d i u ed tac dung ehdng viem d p tren md hinh gay phu chan ehudt bang can-ageenan va gay tran djch mang bung. Tac dung nay tu-ong d u o n g va tham chi cdn tdt hon so vdi indomethacin 5 mg/kg bdi d md hinh gay tran djeh mang bung, cac dich c h i l t t i m phdng edn lam giam sd lugng bach c i u trong dich ri viem, trong khi indomethacin khdng lam thay ddi ehi sd nay. Theo mdt sd nghien cipu, d l lam giam viem trong md hinh gay tran dieh mang bung, d n dung indomethacin vdi lieu 20 mg/kg ^"K Tac dung chdng viem ciia cay t i m phdng d u g c cho la do thanh p h i n triterpenoid ^"i Tren md hinh chdng viem man bang amiant, chi ed djeh c h i l t n u d c t i m phdng la the hien tae dung ire e h l k h i i u, treng khi djeh c h i l t edn khdng t h i hien tae dung nay. Nghien CLPU ciia Sadique cho t h i y cac toan p h i n eay t i m phdng t h i hien tae dung ehdng viem rd vdi l i l u 500 mg/kg. Trong thir nghiem gay u hat thge nghiem, cao t i m phdng ire ehe s g thoat djeh va tang sinh t l bao viem trong viem man tinh, giam lipid peroxidase, giam g-glutamyl transpeptidase va giam heat ddng cua phespholipase A2 trong djeh t i l t u hat '^l
Tae dung c h i n g viem thdng qua giam trpng lugng tuyen ire d u g e xem n h u la mdt md hinh md phdng tac dung ehdng viem thee kieu eertieoid.
Cortieeid la mdt hermon eiia vd thugng than cd tae dung ngan can hinh thanh c a c t i bae m i l n djeh nen CO the gay ly giai lymphe bae va lam teo t u y i n ird^l K i t qua nghien ciru d bang 4 eho t h i y prednisolon vdi lieu 6 mg/kg da lam giam trpng lugng t u y i n u-c rd sau 3 ngay d l l u tri. Djch chiet n u d c va djeh c h i l t cdn t i m phdng da khdng t h i hien rd tac dung n a y
33
Nghien CLFU - Ky thuat
Cae nghien cuu ve co e h l chong viem ciia tam phdng tren t h i gidi cho t h i y u-c e h l phespholipase A2, ue che C 0 X 2 , giam TNFa va giam NO tai md viem la nhu-ng dieh tae dung quan trpng eiia djeh ehilt t i m phdng. Chua cd nghien eu-u nao chi rd thanh p h i n boat c h i t trong eay t i m phdng ed tac dung ehdng viem.
Ket luan
- Djeh chilt nude va djeh chilt edn eay t i m phdng (lilu 10 g/kg) ehua the hien heat tinh khang k h u i n tren cac ehiing vi k h u i n £ coliva Samoneila sp..
- Ca djch chiet n u d c va djch e h i l t con eay t i m phdng (lilu 10 g/kg) d i u t h i hien tac dung chdng viem c l p qua md hinh gay phii chan chudt bang carrageenan va gay tran djch mang bung bang hen hgp carrageenan + formaldehyd.
- Chi ed djch c h i l t n u d e t i m phdng 10 g/kg t h i hien tac dung chong viem man thdng qua lam giam trpng lugng khdi u tren md hinh gay u hat thge nghiem bang d y amiant d u d i da.
Ca djch ehilt n u d e va dich c h i l t cdn t i m phdng (lilu 10 g/kg) d i u khdng anh h u d n g d i n trpng lugng t u y i n uc sau khi cho ehudt udng thudc lien tuc treng 3 ngay.
T a i lieu t h a m k h a o
1. B5 Huy Bich, Dang Quang Trung, NguySn Thirgng Dong (2006), Cay thuoc va ddng vat lam thudc a Viet Nam, tap 2, Nha xuat ban Khoa hpc ky thuat, trang 794-795.
2. Le Thj DiSm Hong, Nguyen Xuan Thing (2003),
"Gop phan tim hieu tac dung chong viem cua hoa cay {Ngay nhan bai: 20/03/2016
kim ngan (/.on/cerayapon/ca Thunb.)", Tap chi Dugc hoc s64/2003, trang 13-15.
3. H. Gerhard Vogel (2002), "Drug discovery and evaluation, pharmacological assays", Springer-Veriag Beriin Haldelberg, pp. 725-771.
4. Jigna Parekh, Sumltra V. Chanda (2006), "In vito antimicrobial activity and phytochemlcal analysis of some Indian medicinal p\ani\TurkJ. Biol., 31 (3), pp 53-58.
5. M. S. Sheeba and V. V. Asha (2006), "Effect of Cardiospermum halicacabum L. on ethanol-induced gastric ulcer in rats", J. of Ethnopharmacology, volume 106, Issue 1, pp. 105-110.
6. Sadique, J. Chandra, T. Thenmozhi, V. Elango (2007), "Biochemical models of action of Cassia occidentalis and Cardiospermum halicacabum in inflammation". International Bibliographic information Dietary Supplements, 19(2), pp. 201-212.
7. 8. M. Jachak, C. Selvam, A. 8. Rivastava, V.
Ahuja (2007), "Evaluation of anti-inflammatory activity and Identification of bioactive compounds from Vitex negundo L., Cardiospermum halicacabum L. and Tridax procumbens L.", Theme Connect, 73.
8. The European agency for the evaluation of medicinal products (1990), "Committee for veterinary medicinal products, Cardiospermum halicacabum L.
report". Veterinary Medicines Evaluation Unit, 664, 99.
9. Venkatesh Babu K. C. and Krishnak U. M.
(2006), "Cardiospermum halicacabum L. suppresses the production of TNF-alpha and Nitric oxide by human peripheral blood mononuclear calls", African J. of Biomedical Research, Vol.9, pp. 95-99.
10. Z. Phytother (2005), "Treatment of eczema with Cardiospermum halicacabum L., Cardiospermum ointment and ointment based on a part-place comparison controlled trial", Saibbe and Sallbengrundlage in Halbseitenverglerich, 5, pp. 263-266.
• Ngay duyet dang: 25/04/2016) r
Nghien cmi tac dung giam dau va chong viem cua cay goi hac
Nguyin Thi Phu-ffng'*, Nguyin Thuy Du-ong^ Dau Thi Giang^
Nguyin Minh Khoi', Phu-ong Thien Thu'o'ng'
' Vien Duac lieu
- Truang Dai hoc Duac Hd Npi ' E-mail: phuong? 6. hpttc@gmail. camSummary
The analgesic and anti-inflammatory activities ofLeea mbra Blume (Leeaceae) extracts were investigated in mice and rats. Acute and chronic anti-inflammatory activity was estimated by the model of earrageenan- induced paw edema and cotton pellets in rats. Analgesic effect was tested in mice by hot plate and writhing test to distinguish the central and peripheral effects, respectively In writhing tests, effects of the ethanol extracts of root and leaf were significant at doses of 100; 200 and 400 mg/kg. By the model of canrageenan-indueed paw edema, both root nad leaf extracts showed a trend to reduce the edema at doses of 100 and 200 mg/kg
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