VIEN C H A N N U O I - Tgp chi Khoa hgc Cdng nghi Chdn nudi - Sd 12-Thang 6-2008
DANH GIA TY LE
TIEU HoA
CHATHfru CO vA GiA TRI
NANG LU^ONG T H i r c A N T H O C U A G I A s u e N H A I L A I B A N G K Y T H U A T T I E U H O A IN VITROv d i NGUON D U O N G C H A T C H O VI SINH VAT TlT DICH DA CO
Danh Mo' vd Nguyen Vdn Thu'*
'BO mon Nong nghiep, Khoa Ky thuat cdng nghe, Cao ding cong dong Kien Giang
^Bo mdn Chan nudi, Khoa Ndng nghiep va Sinh hoa irng dung, Dai hoc Can Tho.
Khu n , Dudng 3/2, P.Xuan Khanh, Q. Ninh Kieu, TP. Cin The;
*Tac gia lien he: - Nguyen Van Thu : Tel: 0918.549.422; Email: [email protected] -DanhMd : Tel; 0919.210.291; Email: [email protected]
ABSTRACT
Evaluation of organic matter digestibility and metabolizable energy of ruminant feedstuffs by the in vitro digestion technique with nutrient sources for microbes from rumen fluid
Three experiments were carried out at Cantho University in Mekong delta, Vietnam to evaluate 69 ruminant feedstuffs by the in vitro digestion technique of using rumen fluid (RF) from buffaloes, cattle and goats as nutrient sources for microbes. The results found that the feed organic matter digestibility (OMD) and metabolizable energy (ME) evaluated by the in vitro digestion technique of using 42ml RF plus 8ml buffer solution as medium for microbes (42RF) had a closely linear relationship to those obtained by the In vitro technique standardized by Goering and van Soest (GvS) with the R^> 0.919. The calculated ME value by basing on chemical composition, and OMD of the 42RF at 72h and GvS at 48h were not significantly different (P= 0.878), The conclusions were that the RF from buffaloes, cattle and goats could be used as nutrient sources for microbes to measure the In vitro feed digestibility. The 42RF in vitro technique'had a high potential to evaluate OMD and ME of ruminant feedstuffs.
Keywords: In vitro digestibility, rumen fluid, nutrient sources, microbes
DATVANDE
Danh gia chat lugng thiic an bang phuong phap xac dinh ti Id tieu hoa Uong phdng thi nghiem {in vitro) de xuat bdi Goering va van Soest (1970) dugc quan tam ling dung nhd cd tdc do nhanh, hieu suat cao va tin cay (Nguyen Van Thu, 2002; Danh Md va Nguyen Van Thu, 2003;
Robinson va cs, 2004). Ddng thdi nd thudng dugc diing lam nen tang de danh gia chat lugng ciia cac thirc an khac nhau vdi cac ky thuat in vitro hien hiiu (Mabjeesh va cs, 2000;
Al-Masri, 2003; Nguyen Van Thu va Uden, 2003).
Tuy nhien, ky thuat nay van tdn tai nhiing bat lgi do sir dung nhieu loai hoa chat lam dudng chat cho vi sinh vat, ma cac loai hoa chat nay la khan hiem ya tdn kem trong dieu kien nude ta (Nguyen Van Thu, 2006).
Dich dg co (DDC) da dugc biet chiia nhieu dudng chat nhu ammonium, peptide, acid amin, acid beo bay hoi, khoang, vitamin va cac tien td khac dugc hinh thanh tir su phan huy thiirc an va sir tdng hgp ciia vi sinh vat da cd (Kamra, 2005). Cho nen chiing ta cd the tan dung lam mdi trudng dudng chat cho vi sinh vat va ludn san cd d Id md (Nguyen Van Thu, 2006).
Nhiing nghien cim gan day (Danh Mo va Nguyen Van Thu, 2007) da cho thay tiem nang ciia DDC lam ngudn dudng chat cho vi sinh vat de xac dinh ti le tieu hoa thiic an d in vitro.
Do vay, nghien ciiu nay nham muc dich xac dinh kha nang irng dung ky thuat diing DDC ciia Uau, bd va de lam ngudn dudng chat cho vi sinh vat phat trien, de danh gia sir tieu hoa chat hiiu CO va gia tri nang lugng cua thiic an.
VAT LIEU vA PHUONG P H A P NGHIEN CUtJ Vat lieu nghien cuu
Doi tuffng nghiin ciru: Cac gia siic diing de cung cap DDC chi dugc cho an cd tu nhien va dugc thu lay tir Id md.
Bo tri thi nghiem : gdm cd 3 thi nghiem (TN) vdi 69 mau thirc an dugc bd tri nhu sau:
TN 1: cd 32 mau thirc an thudc nhdm cd hg hoa thao, cd hg dau, phu pham, day bau bi, thuy thuc vat va nhdm thirc an khac vdi ngudn DDC sir dung la ciia trau;
TN 2: cd 16 mau thirc an thudc nhdm cd hg boa thao, cd hg dau, phu pham va nhdm thirc an khac vdi ngudn DDC sir dung la cua de
TN 3 : cd 23 mau thirc an thudc nhdm cd hg hoa thao, phu pham trdng trgt, phu pham che bien thuc pham, cd hg dau, banh dau va nhdm thirc an khac vdi ngudn DDC sir dung la ciia bd.
Cac chi tieu theo doi
J • •
Thanh phin hoa hgc cua cac loai thiic an bao gdm vat chat khd (DM), chat hiru co (OM), dam thd (CP), beo (EE) va xo thd (CF) phan tich theo AOAC (1980), % xo trung tinh (neutral detergent fiber, NDF) va xo axit (acid detergent fiber, ADF) phafi tich theo phuang phap Van Soest vacs. (1991).
Ti le tieu boa chit him co (OMD) in vitro, dugc md ta bdi Goering va van Soest (1970).
Gia tri nang lugng trao ddi (ME), dugc udc tinh theo he thdng ADAS ciia Anh nhu theo (Robinson va cs, 2004) md ta nhu sau:
ME = 0,82 X [0,24 CP + 0,39 EE + 0,18 (OM - CP -EE)] x OMD in vitro.
Gia tri OMD d TNI va TN2 xac dinh d thdi didm 0; 48 va 96 gid va d TN3 xac dinh d thdi didm 0; 24; 48 va 72gid.
Phugng phap nghien cii-u
Danh gia bing 2 ky thuat tieu boa in vitro song song la: 1) Theo dd nghi ciia Goering va van Soest (1970) [GvS]; 2) Bdi ky thuat sir dung 42ml DDC va 8ml dung dich dem (42DDC) thay cac hoa chat trypticase, cac loai khoang da-vi lugng lam dudng chat cho vi sinh vat.
Ky thuat tieu hoa in vitro 42DDC dugc md ta vdi cac budc chinh nhu sau:
Budcl: Can khoang 0,5g mau (mau da dugc nghidn d kich thudc 1mm) [khdi lugng, TLm]
cho vao dng nghiem 175ml
Suae 2: Cho 8ml dung dich dem va 42ml DDC vao dng nghiem da cd mau
Buac 3: Tao ydm khi bing each cho 2ml dung djch oxy hoa khir (reducing) va bom khi CO2 Buac 4: Cac ong nghiem nay dugc ii trong ndi chung each thuy (water bath) d 39-40°C trong khoang thdi gian nhu dien ta phia tren
Budc 5: Cac dng nghiem dugc liy ra khdi ndi chung each thuy, xu ly ngay vdi 50ml dung dich tiy trung tinh (neutral detergent solution) d 85°C trong khoang 12 gid
Bu&c 6- Chuydn toan bd vat chit trong dng nghiem vao cdc lgc (crucible) de rira vdi nude ndng va acetone. Siy cdc lgc d 105°C Uong khoang 12 gid va can (KLl). Tiep tue dem cdc Igc di nung d 500°C trong 2 gid va can (KL2)
VIEN C H A N N U O I - Tgp chi Khoa hgc Cdng nghi Chdn nudi - Sd 12-Thang 6-2008
Suae 7; Tinh OMD % = 100- [(KL1-KL2) / (KLm x DM x OM)] x 100.
Ddi vdi ky thuat tieu hoa in vitro GvS, d Budc 2 la sir dung 32ml dung dich dudng chat (tryticase va cac loai mudi khoang) + 8ml dung dich dem + 10ml DDC cho vao dng nghiem da cd mau. Cac budc tiep theo tuong tu nhu ky thuat in vitro 42DDC. Dich da cd dugc lgc qua 3 lap vai muslin trudc khi dimg de thue hien cac thi nghiem.
Xir ly so lieu
Cac sd lieu dugc sir ly tren may tinh bang phan mem Minitab. 13 KET QUA vA T H A O LUAN Thi nghiem 1
Bang 1: Thanh phan hoa hgc (%DM) ciia cac loai thiic an diing Uong TNI Nhom thuc jen thirc an DM OM CP NDF CF EE an Ho hoa Than bo bo
thao Cd chi Cd Idng tay Cd Idng vuc Cd mat Cd mdm Cd nude man Cd dng Cd voi Cd sa
Cd Paspalum Nggn-la say Cd Vetiver Ho dau La binh linh
Day dau diia La dien dien Dau Macro La so diia
27,8 25,2 20,7 23,5 24,4 22,0 25,5 20,4 20,2 20,5 13,1 29,3 35,6 28,9 18,7 22,1 12,7 24,5
90,1 88,0 89,5 86,2 89,8 89,1 84,1 91,9 87,9 87,7 87,1 89,6 92,0 91,7 90,1 91,0 91,1 91,0
8,60 12,0 7,66 13,7 12,3 9,56 6,33 9,69 9,79 6,72 6,47 9,32 4,39 19,3 12,4 17,9 15,2 21,9
73,3 68,2 70,1 61,1 68,1 69,5 65,4 72,9 67,1 71,8 67,4 65,8 80,3 38,2 40,9 38,4 47,8 27,5
26,4 24,6 29,9 25,2 24,1 23,6 18,0 31,7 30,3 34,3 31,2 27,6 36,1 16,6 13,4 17,4 26,6 13,7
4,00 5,82 4,56 2,20 4,44 3,43 4,91 3,01 1,49 2,77 2,72 3,69 1,45 8,42 4,21 3,95 3,31 7,43
7,66 9,92 9,41 8,83 9,62 8,99 8,81 8,47 9,26 7,29 8,86 7,85 4,60 12,5 11,3 11,3 10,6 13,9 Phu pham
Ho bau bi
Thuy thuc vat
Nhdm khac
Than-la bap gia Than-la bap non Vd khdm Rom Day bau Day bi dao Day bi dd Day dua hau Beo tam Luc binh Rau mudng La tre Cd nang Than nghe
15,1 17,4 9,25 64,9 7,65 11,4 16,6 10,4 4,44 7,07 9,92 33,4 27,4 20,9
91,0 92,7 94,1 85,2 82,3 81,8 84,0 83,4 78,2 81,0 89,6 88,9 88,6 91,0
9,79 9,86 4,23 3,51 17,5 15,5 17,4 22,4 28,0 15,1 16,0 14,6 9,00 22,8
65,3 65,1 42,2 72,4 48,0 40,8 26,0 44,4 23,1 58,5 29,7 71,5 70,6 59,2
36,9 33,9 12,1 36,4 20,4 15,4 6,94 21,4 7,15 19,7 10,6 33,7 25,6 19,2
2,33 5,68 5,49 1,78 3,19 5,43 5,19 9,43 5,68 1,17 4,87 1,63 1,52 1,19
8,20 8,68 11,7 6,97 8,81 8,52 12,3 11,0 11,6 9,64 13,1 7,32 6,28 7,01
Ghi chii: DM: chdt khd. OM: chdt huru ca; CP: dgm thd; EE: beo; CF: xa thd; NDF (neutral detergent fiber): xa trung tinh; ME: ndng luang trao ddi uac tinh dua tren OMD 48gia cua ky thudt in vitro Goering vd van Soest (1970) vd thdnh phdn hod hgc theo di nghi Robinson vd cdng su (2004)
Ciing thai diem tieu boa gia tri OMD cua ky thuat tieu hoa in vitro 42DDC khac biet cdy nghTa thdng ke (P<0,001) (Bang 2) va cd mdi quan he tuydn tinh gin vdi OMD cua ky thuat tieu hoa in vitro GvS (Bang 3). Nen kdt luan la cd thd img dung ky thuat 42DDC dd xac dinh OMD thirc an d in vitro.
Bang 2: Su khac biet vd gia tri fi le tieu hoa chit him co (OMD, %) giiia 2 ky thuat fieu hoa in vitro 42DDC va GvS trong TN 1
Chi tieu OMD Ogid OMD 48gid OMD 96gid
Ky thuat in vitro 42DDC
31,7 61,0 65,9
Ky thuat in vitro GvS
35,0 65,4 70,8
SE 5,08 4,18 4,29
P
< 0,001
< 0,001
< 0,001
Ghi chii: 42DDC: ky thiidi tieu hod in vitro vai ngudn duang chdt cho vi sinh vdt tic 42ml dich dg cd trdu + 8ml dung dich dem; GvS: ky thudt lieu hod in vitro di xudt bdi Goering vd van Soest (1970)
Bang 3: Mdi quan he tuydn tinh vd gia tri ti le tieu hoa chit hiru co (OMD, %) giira 2 ky thuat tieu hoa in vitro 42DDC va GvS trong TN 1
Chi tieu OMD Ogid OMD 48gid OMD 96gid Tdng
n 32 32 32 96
Phuong trinh
Y G V S = 1,06 X42DDC+ 1,48 YGVS = 1,02 X42DDC + 3,12 Y G V S = 1,01 X42DDC + 4 , 2 5 Y G V S = 1,04 X42DDC + 2 , 1 3
R^
0,903 0,899 0,880 0,954
SE 5,09 4,25 4,36 4,51
P
< 0,001
< 0,001
< 0,001
< 0,001
Ghi chu: 42DDC: ky thudt tieu hod in vitro vai ngudn duang chdt cho vi sinh vdt tir 42ml dich dg co trdu + 8ml dung dich dim: GvS: ky thudt tiiu hod in vitro de xudt bai Goering vd van Soest (1970)
Thi nghiem 2
Bang 4: Thanh phan hoa hgc (%DM) ciia cac loai thiic an diing Uong TN2 Nhdm thirc an
Hg hoa thao
Hg dau
Phu pham
Ten thiic an Cay nga Cd Idng tay Cd mdm Sorghum Dau bide Dau Idng Stylosanthes La cai xanh La cu cai Vd chudi Vd-xo mit
DM 21,0 20,7 22,0 34,0 13,9 7,95 16,2 9,44 5,88'
16,0 12,5
OM 92,8 89,5 91,2 92,0 91,4 89,0 91,7 67,9 67,3 77,9 91,2
CP 6,00 7,66 9,65 6,26 16,6 17,7 12,7 18,6 19,8 7,52 9,05
EE 3,14 4,56 4,44 3,32 2,66 4,32 1,91 7,69 7,22 1,45 6,45
NDF 74,6 70,1 69,5 74,1 56,4 52,7 55,9 37,5 36,8 46,6 34,4
CF 40,1 29,9 23,6 35,5 37,1 32,6 37,0 16,0 18,8 16,6 25,5
ME,MJ /kgDM 6,00 8,72 8,46 7,72 8,38 9,54 8,70 10,1 9,83 7,33 9,85
VIEN C H A N N U O I - Tgp chi Khoa hgc Cdng nghi Chdn nudi - S6 12-Thang 6-2008
Nhdm thirc an Nhdm khac
Ten thiic an Cd siia Dia ciic Day dua gan La khoai mi Ladi
DM 19,1 10,4 14,6 90,6 27,4
OM 88,7 81,4 78,9 88,8 92,1
CP 11,9 12,7 12,3 19,0 12,3
EE 1,74 2,34 3,35 7,36 2,07
NDF 39,2 38,2 44,5 48,7 64,3
CF 20,4
19,9 21,8 15,1 18,9
ME,MJ /kgDM 10,5 9,88 11,5 8,48 5,81 Ghi chii: DM: chdt khd, OM: chdt hiru ca; CP: dgm thd; EE: beo; CF: xa thd; NDF (neutral detergent fiber): xa trung tinh; ME: ndng lugng trao ddi uac tinh dua tren OMD 48gid ciia ky thudt in vitro Goering vd van Soest (1970) vd thdnh phdn hod hgc theo de nghi Robinson vd cdng su (2004)
Bang 5: Sir khac .biet ve gia tri ti le tieu hoa chat hiru co (OMD, %) giira 2 ky thuat tieu hoa in vitro 42DDC va GvS trong TN 2
Chi tieu OMD Ogid OMD 48gid OMD 96gid
Ky thuat in vitro 42DDC
39,2 53,3 61,2
Ky thuat in viUo GvS
41,4
64,1 ' 68,9
SE 2,88 6,80
^ 6,03
P 0,008
< 0,001
< 0,001 Ghi chii: 42DDC: ky thudt tieu hod in vitro vdi ngudn duang chdt cho vi sinh vdt tir 42ml dich dg cd de + 8ml dung dich dim; GvS: ky thudt lieu hod in vitro di xudt bai Goering vd van Soest (1970) -
Bang 6: Mdi quan he tuyen tinh ve gia tri ti le tieu boa chat hiiu co (OMD, %) giira 2 ky thuat tieu boa in vitro 42DDC va GvS trong TN 2
Chi tieu OMD Ogid OMD 48gid OMD 96gid Toan bd
n 16 16 16 48
Phuong trinh
YGVS = 0,986 X425DC + 2,74
YGVS = 0 , 9 2 1 X 4 2 D D C + 1 5 , 0 YGVS = 0,875 X42DDC+15,4
YGVS= 1,05 X42DDC +4,52
R^
0,963 0,774 0,797 0,876
SE 2,97 6,96 6,01 6,45
P
< 0,001
< 0,001
< 0,001
< 0,001 Ghi chii: 42DDC: ky thudt tieu hod in vitro vdi ngudn duang chdt cho vi sinh vdt tir 42ml dich dg cd de + 8ml dung dich dim; GvS: ky thudt tieu hod in vitro di xudt bai Goering vd van Soest (1970)
Trong ciing thdi diem tieu hoa cho thay gia tri OMD cua ky thuat tieu boa in vitro 42DDC khac biet cd y nghia thdng ke (P<0,001) (Bang 5) va cd mdi quan he tuyen tinh chat che vdi OMD ciia ky thuat tieu boa in vitro GvS (Bang 6). Cho nen ket luan la ky thuat tieu hoa in vitro 42DDC cd the danh gia dugc OMD thiic an thay cho ky thuat tieu hoa in vitro GvS.
Thi nghiem 3
Thanh phan hoa hgc ciia cac loai thiic an trong TN3 trinh bay d Bang 7.
Bang 7: Thanh phan hoa hgc (%DM) cua cac loai thiic an diing trong TN3 Nhdm thiic an
Hg hoa thao
Ten thiic an Cd voi
Cd Idng tay Cd man trau Cd vetiver
DM 26,5 26,6 18,6 35,2
OM 89,1 91,4 89,7 92,0
CP 8,83
13,2 14,2 6,73
EE 4,81 6,13 4,51 2,44
NDF 67,6 70,2 68,0 78,4
ADF 39,9 33,6 35,8 44,7
ME,MJ /kgDM 9,85
10,0 10,6 4,21
Nhdm thirc an Phu pham trdng trgt
Phu pham che bien
Hg dau
Banh dau
Loai khac
Ten thirc an Rom
Cay bap tuoi Nggn mia Than chudi Ba dau nanh Ba mia Ba khdm Ba bia La binh linh Nggn dien dien La so diia Cay dau phdng Banh dau dau nanh Banh dau bdng vai Banh dau dira Trichantera Dia cue Cay nd Day bim bim
DM 90,6 24,9 21,0 6,57 12,1 39,6 12,1 23,5 22,5 22,5 20,7 21,3 87,9 91,3 91,4 15,1 14,0 21,1 18,5
OM 88,7 93,6 94,3 84,5 97,7 98,4 93,3 95,7 89,7 92,2 90,0 88,2 91,3 95,7 94,2 79,2 82,6 81,3 90,6
CP 5,73 7,29 4,70 2,75 24,9 2,51 6,01 25,4 19,1 16,8 22,2 11,7 38,7 21,8 16,4 13,6 11,0 14,2 13,4
EE 3,30 5,33 3,80 6,00 13,4 1,42 11,3 12,8 8,66 6,42 9,48 5,56 5,66 11,6 13,3 9,32 8,12 5,46 6,12
NDF 72,2 55,7 61,1 57,3 44,1 61,2 44,6 71,4 41,3 47,1 30,8 44,5 35,2 48,3 57,8 37,6 34,6 49,3 46,4
ADF 39,9 32,0 36,1 42,2 20,0 40,6 21,2 38,6 23,0 35,0 24,1 35,5 20,2 43,2 33,4 24,0 28,6 30,0 34,6
ME,MJ /kgDM 7,50
10,7 7,90 9,49 14,3 8,14 12,5 6,45 10,8 11,0 12,9 10,9 14,1 11,2 10,9 10,4 12,0 9,80 11,2
Ghi chu: DM: chdt khd, OM: chdt huru ca; CP: dam thd: EE: beo; NDF (neutral detergent fiber): xa trung tinh;
ADF (acid detergent fiber): xa axit; ME: ndng luang trao ddi uac tinh dua tren OMD 48gia cua ky thudt tn vitro Goering vd van Soest (1970) vd thdnh phdn hod hgc theo de nghi Robinson vd cgng su (2004)
Trong ciing thdi diem tieu hoa, gia tri OMD cua ky thuat tieu boa in vitro 42DDC thap ban GvS ed y nghTa thdng ke (P<0,001), d 24 gid tieu hoa chiing tdi chua tim dugc sir khac biet cd y nghia (P= 0,063). Kdt qua d (Bang 8).
Bang 8: Sir khac biet ve gia tri ti le tieu hoa chat hiiu co (OMD, %) va nang lugng trao ddi (ME, MJ/kgDM) giira 2 ky thuat tieu hoa in vitro 42DDC va GvS trong TN3 Chi tieu
OMD Ogid
OMD 24gid : OMD 48gid
OMD 72gid ME*
Ky thuat in vitro GvS
46,9 61,7 69,2 73,4
10,2 (48gid)
Ky thuat in vitro 42DDC
44,6 59,7 66,3 69,9
10,2 (72gia)
SE 2,52 5,06 4,10 5,35 0,137
P
< 0,001 0,063 0,003 0,005 0,878
Ghi chu: 42DDC: ky thudt tieu hod in vitro vdi ngudn ducmg chdt cho vi sinh vdt tic 42ml dich dg cd bd + 8ml dung dich dem; GvS: ky thudt tieu hod in vitro de xudt bai Goering vd van Soest (1970); "uac tinh dua tren OMD 72gid (ky thudt tieu hod in vitro 42DDC; hoac OMD 48gid (ky thudt tieu hod in vitro GvS) vd thdnh phdn hod hgc theo de nghi Robinson vd cdng su (2004)
Trong TN3 chiing tdi cdn thiy dugc ring OMD 72gid cua ky thuat tieu hoa in vitro 42DDC khac biet khdng y nghTa thong ke (P=0,390) so vdi OMD 48gid ciia ky thuat tieu hoa in vitro GvS. Gia tri ME dugc du doan dua tren OMD 72gid ciia ky thuat tieu hoa in vitro 42DDC va OMD 48gid ciia ky thuat fieu hoa in vitro GvS khac nhau khdng y nghia thdng ke (P=0,878).
VIEN CHAN NUOI - Tgp chi Khoa hgc Cdng nghi Chdn nudi - S6 12-Thang 6-2008
Ky thuat tieu hoa in vitro 42DDC ciing cd mdt mdi quan he tuydn tinh chat che vdi ky thuat tieu hoa in vitro GvS (Bang 9). Do vay TN3 ket luan rang ky thuat tieu boa in vitro 42DDC danh gia dugc OMD va ME thiic an thay cho ky thuat tieu boa in vitro GvS.
Bang 9: Mdi quan he tuyen finh ve gia tri fi le tieu hoa chat hiru co (OMD, %) va nang lugng trao ddi (ME, MJ/kgDM) giira 2 ky thuat tieu hoa in vitro 42DDC va GvS trong TN3
Phuong trinh R^ SE
2 3 Y c v S O M D O g ^ 1,01 X42DDC_OMD Og + 1,90 2 3 YGVS OMD24g = 0 , 9 1 4 X42DDC_OMD 24g + 7 , 1 9 2 3 YGvS_OMD48g = 0 , 8 9 8 X42DDC_0MD 48g + 9 , 6 1 2 3 YGVS OMD 72g = 0 , 9 1 0 X42DDC_0MD 72g + 9 , 7 3 2 3 YGvS_ME48g==0,939X42DDC_ME72g + 0 , 6 1 3 9 2 YGVS OMD Q-72g = 0 , 9 5 6 X42DDC OMD 0-72g + 5 , 2 8
0,977 0,933 0,948 0,897 0,919 0,951
2,57 4,89 3,78 5,26 0,656
4,30
< 0,001
< 0,001
< 0,001
< 0,001
< 0,001
< 0,001
Ghi chii: 42DDC: ky thudt tieu hod in vitro vai ngudn duang chdt cho vi sinh vdt tic 42ml dich dg cd bd + 8ml dung dich dim; GvS: ky thudt tieu hod in vitro di xudt bdi Goering vd van Soest (1970)
C/3
6
c d - = J
o -O •5b
NO 0 ^
Q
o
120 n 100 -
80 - 6 0 - 40 - 20 -
Y = 1,07X
R= = 0 , 9 2 8 , S E = 5 , 3 6 ; n = 2 3 6
y = 1,00X-H 4 , 6 0 R = = 0 , 9 3 4 ; S E = 5 , 1 5 ; n = 2 3 6
— I —
20
— I —
40
— I —
6 0 8 0 100 8 0
O M D 0 - 9 6 g i d ciia 4 2 D D C , %
120
D6 thj 1: M6i quan he tuySn tinh giCTa 2 ky thuat tieu hoa in vitro 42DDC va GvS so lieu tong hgp tir 3 TN
Khi ket hgp so lieu cua ca 3 TN cd so quan sat (n) la 236 de phan tich mdi quan he giira hai ky thuat tieu boa in vitro 42DDC va GvS da cho thiy trong trudng hgp phan tich
Ham tuyen tinh y= ax cd gia tri R^ la 0,928 va sai sd (SE) la 5,36 Ham tuyen tinh y= ax + b cd gia tri R^ = 0,934 va SE = 5,15 (Do thj 1).
Vdi gia tri R^ nay da chi ra ring hai ky thuat tieu hoa in vitro 42DDC va GvS cd mdi quan he rit chat che vdi nhau. So vdi cac ky thuat danh gia chat lugng thiic an dugc chap nhan iing dung hien nay nhu sinh khi in vitro (Al-Masri, 2003), tieu hoa in sacco (Nguyen Van Thu, 2002), tieu hoa in vitro vdi ngudn vi sinh vat tir phan (Nguyen Van Thu va Uden, 2003) va tieu boa in vitro DAISY" (Mabjeesh va cong su, 2000) dat dugc gia tri R^ lan lugt d miic 0,81; 0,83; 0,94 va 0,81 khi phan tich mdi quan he tuydn finh vdi ky thuat fieu hoa in vitro GvS. Nhu thd, ky thuat tieu hoa in vitro 42DDC cd tidm nang Idn trong iing dung de danh gia chat lugng thirc an thd diing cho gia siic nhai lai.
KET LUAN vA DE NGHI Ket luan
Djch da cd trau, bd va de d Id mo ddu cd the tan dung nhu la nguon duong chit cho vi sinh vat de xac djnh ti le tieu boa chat hiiu co ciia thiic an d in vitro.
Ky thuat tieu boa in vitro 42DDC ed fiem nang ldn diing dd danh gia OMD va ME thirc an thay cho ky thuat tieu boa in vitro GvS.
De nghj: Cho phep iing dung ky thuat nay trong cac phdng thi nghiem.
T A I LIEU THAM K H A O
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AOAC, (1980). Official Methods of Analysis. 13"' ed. Association of Official Analytical Chemist, Washington,
Danh Mo va Nguyin Van Thu (20()3). M6i lien he gi&a phuang phap xAc djnh kha nSng tieu hoa in vivo v6i in vitro va anh hucmg ciia su b6 sung thiic an dam tren tieu hoa in vitro cua rom va co voi a trau ta. Tap chi Khoa hoc Dai hoc CSn Tho, pp: 62-66.
Danh Mo and Nguyen Van Thu (2007). Results of studies on applications of in vitro digestibility techniques to evaluate ruminant feeds at cantho university, Vietnam, The S"' Vietnamese - Hungarian Conference on Animal Production and Aquaculture, Cantho University, pp: 102-122,
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luong sir tieu hoa thirc an a trau ta DBSCL, Tap san KHKT Nong Lam nghiep 3, Dai hoc Nong Lam Tp, HCM, pp: 85-88.
Nguyen Van Thu (2006), Study of using rumen fluid as nutrient sources for the in vitro digestibility measurement in swamp buffalo. Proceeding of The S"" Asian Buffalo Congress (Yang Bingzhuang, Ed.), April 18-22, 2006, Naning, China, pp: 356-385.
Nguyen Van Thu and Uden, P. (2003). Feces as an alternative to rumen fluid for in vitro digestibility measurement in temperate and tropical ruminants. Buffalo J, 1, pp : 9-17. , ,
Robinson, P, H., Givens, D. 1, and Getachew, G. (2004). Evaluation of NRC, UC Davis and ADAS approaches to estimate the metabolizable energy values of feeds at maintenance energy intake from equations utilizing chemical assays and in vitro determination, Anim, Feed Sci, TechnoL, 114, pp: 75-90.
Van Soest, P, J,, Robertson, J, B, and Lewis, B. A. (1991). Symposium: Carbohydrate methodology, metabolism and nutritional implications in dairy cattle: methods for dietary fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, pp: 3585-3597.
"Ngu-fri ph^n bifn; TS. Dinh Van Tuyin ; TS. D6 Thj Thanh Van
