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Fig. 1. Multiple alignment of amino acid sequence deduced from nucleotide sequence of pEuNOD-AS1 with ASs of nodule-form-ing leguminous plants using Clustal W
Fig. 2. Expression of AS in different organs of Estained with ethidium bromide before blotting to ensure equalloading of RNA (B)
Fig. 4. In situ localization of AS transcript in longitudinal sections of E. umbellata root nodules
Fig. 5. Genomic Southern hybridization of AS. GenomicDNAs (10high stringency. E,rated on 1% agarose gel and corresponding nylon membranewas probed with �g) digested with restriction enzymes were sepa- 32P-labeled insert of pEuNOD-AS1 under EcoRI; H, HindIII; Bg, BglII.

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