LAMPIRAN A. Prosedur Pembuatan Bahan Uji
a. Pembuatan Monosodium Glutamat (MSG)
ditimbang
dimasukkan ke dalam
beaker glass
ditambahkan
aquadest
diaduk menggunakan spatula
b. Pembuatan Vitamin C
ditimbang
dimasukkan ke dalam
beaker glass
ditambahkan
aquadest
diaduk menggunakan spatula
c. Pembuatan Vitamin E
diambil menggunakan spit
dilarutkan dengan minyak jarak (
oleum ricini)
LAMPIRAN B. Pembuatan Preparat Histologis Ginjal
Dibilas dengan NaCl 0,9%
Difiksasi dalam BOUIN selama 1 malam
Washing
dalam alkohol 70%
Dehidrasi dengan alkohol konsentrasi bertingkat mulai 70%,
80%, 96%, dan 100%
Clearing
dalam xylol
Infiltrasi
Embedding
(penanaman) organ dalam cetakan kemudian
dituangkan paraffin murni, dibiarkan hingga didapatkan blok
paraffin
Cutting
(pemotongan) menggunakan mikrotom sehingga
didapatkan pita-pita paraffin
Attaching
(penempelan) pita paraffin pada
object glass
Deparafinasi dengan mencelupkan objek dalam xylol
Dealkoholisasi dalam alkohol menurun dari 100%, 96%, 80%,
hingga 70%
Pewarnaan dengan mencelupkan dalam Hematoxilin selama 3-7
menit kemudian dibilas dengan air mengalir. Selanjutnya
dicelupkan dalam alkohol 70%, lalu dicelupkan dalam Eosin
selama 1-3 menit.
Mounting
yaitu menutup preparat dengan gelas penutup yang
sebelumnya diberi Canada balsam
Diberi label dan siap diamati
Hasil
Pita Paraffin
Blok Paraffin
LAMPIRAN C. Alat Dan Bahan Penelitian
Tempat pemeliharaan
Castrol Oil
Vitamin E
hewan percobaan
Hewan percobaan Dissecting set Bak bedah
(Mus musculus
L.)
LAMPIRAN D. Data dan Analisis Statistik Berat Ginjal Mencit (Mus musculus
L.)
Rataan Berat Ginjal Mencit (Mus musculus
L.) Setelah Pemberian Perlakuan
Vitamin C dan E Setelah Dipajankan Monosodium glutamat (MSG)
Perlakuan
Ulangan
x±SD
1
2
3
4
5
Hasil Uji Statistik Berat Ginjal Mencit (Mus musculus L.
)
Tests of Normality
Kel_Per
lakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statistic Df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
Berat_Ginjal Based on Mean 2.240 5 24 .083
Based on Median .810 5 24 .554
Based on Median and with
adjusted df
.810 5 9.581 .569
Oneway
ANOVA
Berat_Ginjal
Sum of Squares Df Mean Square F Sig.
Between Groups .010 5 .002 2.482 .060
Within Groups .019 24 .001
LAMPIRAN E. Data dan Analisis Statistik Kerusakan Histologis Tubulus
Proksimal Ginjal Mencit (Mus musculus L.)
Persentase Jumlah Kerusakan Histologis Tubulus Proksimal Ginjal Mencit (Mus
musculus
L.) Setelah Pemberian Vitamin C dan E Setelah Dipajankan
Monosodium Glutamat (MSG)
Perlakuan
Ulangan
x±SD
1
2
3
4
5
Hasil Uji Statistik Kerusakan Tubulus Proksimal Ginjal Mencit (Mus musculus
L.)
Tests of Normality
Tests of Normality
Kel_Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
Kerusakan_Histologis
_Ginjal
Based on Mean 1.499 5 24 .227
Based on Median .421 5 24 .830
Based on Median and
with adjusted df
.421 5 15.466 .827
Kruskal-Wallis Test
Ranks
Kel_Perlakuan N Mean Rank
Kerusakan_Histologis_Ginjal K- 5 6.20
K+ 5 4.80
Chi-Square 25.480
df 5
Asymp. Sig. .000
a. Kruskal Wallis Test
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K- 5 6.20 31.00
K+ 5 4.80 24.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U 9.000
Wilcoxon W 24.000
Z -.731
Asymp. Sig. (2-tailed) .465
Exact Sig. [2*(1-tailed Sig.)] .548a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K- 5 3.00 15.00
P1 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K- 5 3.00 15.00
P2 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K- 5 3.00 15.00
P3 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K- 5 3.00 15.00
P4 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K+ 5 3.00 15.00
P1 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K+ 5 3.00 15.00
P2 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K+ 5 3.00 15.00
P3 5 8.00 40.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal K+ 5 3.00 15.00
P4 5 8.00 40.00
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal P1 5 7.40 37.00
P2 5 3.60 18.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U 3.000
Wilcoxon W 18.000
Z -1.984
Asymp. Sig. (2-tailed) .047
Exact Sig. [2*(1-tailed Sig.)] .056a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal P1 5 7.30 36.50
P3 5 3.70 18.50
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U 3.500
Wilcoxon W 18.500
Z -1.886
Asymp. Sig. (2-tailed) .059
Exact Sig. [2*(1-tailed Sig.)] .056a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal P1 5 8.00 40.00
P4 5 3.00 15.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal P2 5 6.30 31.50
P3 5 4.70 23.50
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U 8.500
Wilcoxon W 23.500
Z -.838
Asymp. Sig. (2-tailed) .402
Exact Sig. [2*(1-tailed Sig.)] .421a
a. Not corrected for ties.
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal P2 5 8.00 40.00
P4 5 3.00 15.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.611
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: Kel_Perlakuan
Mann-Whitney Test
Ranks
Kel_Perlakuan N Mean Rank Sum of Ranks
Kerusakan_Histologis_Ginjal P3 5 7.80 39.00
P4 5 3.20 16.00
Total 10
Test Statisticsb
Kerusakan_Histologis_Ginjal
Mann-Whitney U 1.000
Wilcoxon W 16.000
Z -2.402
Asymp. Sig. (2-tailed) .016
Exact Sig. [2*(1-tailed Sig.)] .016a
a. Not corrected for ties.
