Pengaruh Ekstrak Metanol Daun Suren (Toona sureni Bl Merr) Terhadap Fungsi Hati dan Nilai Darah (Rattus norvegicus L.) Jantan yang Dipapari Karbon Tetraklorida (CCl4)

Lampiran 2. Data Pengamatan Rata-Rata Kadar SGPT, SGOT, Eritrosit

dan Kadar HbTikus putih yang diberi ekstrak metanol suren

dengan dosis 100 mg/kg BB, 150 mg/kg BB,dan 200 mg/kg/BB

Kelompok Rata-rata Kadar SGPT(µ/L)

Rata-rata Kadar SGOT (µ/L)

Rata-rata Jumlah Eritrosit

(x 106/mm3)

Rata-rata Kadar Hb (g/100mL)

C10H 255,35 371,47 7,42 15,42

C24H 359,9 362,75 7,31 12,42

MK10H 242,36 343,22 7,61 13,85

MK24H 211,62 354,97 5,97 13,1

K 139,87 315,02 7,07 11,65

CS100 198,1 272,25 9,74 12,47

CS150 287,25 245,5 6,75 15,65

Lampiran 3. Hasil Analisis SPSS 20

untuk Kadar SGPT

ANOVA

Kadar_SGPT

Sum of Squares Df Mean Square F Sig.

Between Groups 118290,701 7 16898,672 4,040 ,005

Within Groups 100399,936 24 4183,331

Total 218690,638 31

Multiple Comparisons

Dependent Variable: Kadar_SGPT Bonferroni

Std. Error Sig. 95% Confidence Interval Lower

Bound

Upper Bound

C10H

C24H -104,55000 45,73473 ,879 -265,1988 56,0988

MK10H 12,99000 45,73473 1,000 -147,6588 173,6388

MK24H 43,72500 45,73473 1,000 -116,9238 204,3738

K 115,47500 45,73473 ,521 -45,1738 276,1238

CS100 57,25000 45,73473 1,000 -103,3988 217,8988

CS150 -31,90000 45,73473 1,000 -192,5488 128,7488

CS200 27,10000 45,73473 1,000 -133,5488 187,7488

C24H

C10H 104,55000 45,73473 ,879 -56,0988 265,1988

MK10H 117,54000 45,73473 ,470 -43,1088 278,1888

MK24H 148,27500 45,73473 ,097 -12,3738 308,9238

K 220,02500* 45,73473 ,002 59,3762 380,6738

CS100 161,80000* 45,73473 ,047 1,1512 322,4488

CS150 72,65000 45,73473 1,000 -87,9988 233,2988

CS200 131,65000 45,73473 ,231 -28,9988 292,2988

MK10H

C10H -12,99000 45,73473 1,000 -173,6388 147,6588

C24H -117,54000 45,73473 ,470 -278,1888 43,1088

MK24H 30,73500 45,73473 1,000 -129,9138 191,3838

K 102,48500 45,73473 ,968 -58,1638 263,1338

CS100 44,26000 45,73473 1,000 -116,3888 204,9088

CS150 -44,89000 45,73473 1,000 -205,5388 115,7588

CS200 14,11000 45,73473 1,000 -146,5388 174,7588

MK24H

C10H -43,72500 45,73473 1,000 -204,3738 116,9238

C24H -148,27500 45,73473 ,097 -308,9238 12,3738

MK10H -30,73500 45,73473 1,000 -191,3838 129,9138

K 71,75000 45,73473 1,000 -88,8988 232,3988

CS150 -75,62500 45,73473 1,000 -236,2738 85,0238

CS200 -16,62500 45,73473 1,000 -177,2738 144,0238

K

C10H -115,47500 45,73473 ,521 -276,1238 45,1738

C24H -220,02500* 45,73473 ,002 -380,6738 -59,3762 MK10H -102,48500 45,73473 ,968 -263,1338 58,1638

MK24H -71,75000 45,73473 1,000 -232,3988 88,8988

CS100 -58,22500 45,73473 1,000 -218,8738 102,4238

CS150 -147,37500 45,73473 ,102 -308,0238 13,2738 CS200 -88,37500 45,73473 1,000 -249,0238 72,2738

CS100

C10H -57,25000 45,73473 1,000 -217,8988 103,3988

C24H -161,80000* 45,73473 ,047 -322,4488 -1,1512

MK10H -44,26000 45,73473 1,000 -204,9088 116,3888 MK24H -13,52500 45,73473 1,000 -174,1738 147,1238

K 58,22500 45,73473 1,000 -102,4238 218,8738

CS150 -89,15000 45,73473 1,000 -249,7988 71,4988

CS200 -30,15000 45,73473 1,000 -190,7988 130,4988

CS150

C10H 31,90000 45,73473 1,000 -128,7488 192,5488

C24H -72,65000 45,73473 1,000 -233,2988 87,9988

MK10H 44,89000 45,73473 1,000 -115,7588 205,5388

MK24H 75,62500 45,73473 1,000 -85,0238 236,2738

K 147,37500 45,73473 ,102 -13,2738 308,0238

CS100 89,15000 45,73473 1,000 -71,4988 249,7988

CS200 59,00000 45,73473 1,000 -101,6488 219,6488

CS200

C10H -27,10000 45,73473 1,000 -187,7488 133,5488

C24H -131,65000 45,73473 ,231 -292,2988 28,9988

MK10H -14,11000 45,73473 1,000 -174,7588 146,5388

MK24H 16,62500 45,73473 1,000 -144,0238 177,2738

K 88,37500 45,73473 1,000 -72,2738 249,0238

CS100 30,15000 45,73473 1,000 -130,4988 190,7988

CS150 -59,00000 45,73473 1,000 -219,6488 101,6488

Lampiran 4. Hasil Analisis SPSS 20 untuk Jumlah Eritrosit

ANOVA

Jumlah_Eritrosit

Sum of Squares df Mean Square F Sig.

Between Groups 39960221875000,

000 7

5708603125000,0

00 8,818 ,000

Within Groups 15536975000000,

000 24

Dependent Variable: Jumlah_Eritrosit Bonferroni

Std. Error Sig. 95% Confidence Interval

Lower Bound

Upper Bound

C10H

C24H 112500,000 568934,952 1,000 -1885952,86 2110952,86

MK10H -195000,000 568934,952 1,000 -2193452,86 1803452,86

MK24H 1452500,000 568934,952 ,489 -545952,86 3450952,86

K 352500,000 568934,952 1,000 -1645952,86 2350952,86

CS100

-2325000,000* 568934,952 ,012 -4323452,86 -326547,14

CS150 670000,000 568934,952 1,000 -1328452,86 2668452,86

CS200 -1445000,000 568934,952 ,503 -3443452,86 553452,86

C24H

C10H -112500,000 568934,952 1,000 -2110952,86 1885952,86

MK10H -307500,000 568934,952 1,000 -2305952,86 1690952,86

MK24H 1340000,000 568934,952 ,757 -658452,86 3338452,86

K 240000,000 568934,952 1,000 -1758452,86 2238452,86

CS100

-2437500,000* 568934,952 ,007 -4435952,86 -439047,14

CS150 557500,000 568934,952 1,000 -1440952,86 2555952,86

CS200 -1557500,000 568934,952 ,321 -3555952,86 440952,86

MK10H

CS200 -1250000,000 568934,952 1,000 -3248452,86 748452,86

MK24H

C10H -1452500,000 568934,952 ,489 -3450952,86 545952,86

C24H -1340000,000 568934,952 ,757 -3338452,86 658452,86

MK10H -1647500,000 568934,952 ,222 -3645952,86 350952,86

K -1100000,000 568934,952 1,000 -3098452,86 898452,86

CS100

-3777500,000* 568934,952 ,000 -5775952,86 -1779047,14

CS150 -782500,000 568934,952 1,000 -2780952,86 1215952,86

CS200

-2897500,000* 568934,952 ,001 -4895952,86 -899047,14

K

C10H -352500,000 568934,952 1,000 -2350952,86 1645952,86

C24H -240000,000 568934,952 1,000 -2238452,86 1758452,86

MK10H -547500,000 568934,952 1,000 -2545952,86 1450952,86

MK24H 1100000,000 568934,952 1,000 -898452,86 3098452,86

CS100

-2677500,000* 568934,952 ,002 -4675952,86 -679047,14

CS150 317500,000 568934,952 1,000 -1680952,86 2315952,86

CS200 -1797500,000 568934,952 ,119 -3795952,86 200952,86

CS100

C10H 2325000,000* 568934,952 ,012 326547,14 4323452,86

C24H 2437500,000* 568934,952 ,007 439047,14 4435952,86

MK10H 2130000,000* 568934,952 ,028 131547,14 4128452,86

MK24H 3777500,000* 568934,952 ,000 1779047,14 5775952,86

K 2677500,000* 568934,952 ,002 679047,14 4675952,86

CS150 2995000,000* 568934,952 ,001 996547,14 4993452,86

CS200 880000,000 568934,952 1,000 -1118452,86 2878452,86

CS150

C10H -670000,000 568934,952 1,000 -2668452,86 1328452,86

C24H -557500,000 568934,952 1,000 -2555952,86 1440952,86

MK10H -865000,000 568934,952 1,000 -2863452,86 1133452,86

MK24H 782500,000 568934,952 1,000 -1215952,86 2780952,86

K -317500,000 568934,952 1,000 -2315952,86 1680952,86

CS100

-2995000,000* 568934,952 ,001 -4993452,86 -996547,14

CS200

-2115000,000* 568934,952 ,030 -4113452,86 -116547,14

CS200

K 1797500,000 568934,952 ,119 -200952,86 3795952,86

CS100 -880000,000 568934,952 1,000 -2878452,86 1118452,86

CS150 2115000,000* 568934,952 ,030 116547,14 4113452,86

*. The mean difference is significant at the 0.05 level.

Lampiran 5. Hasil Analisis SPSS 20untuk kadar SGOT

ANOVA

Kadar_SGOT

Sum of Squares Df Mean Square F Sig.

Between Groups 82447,565 7 11778,224 1,618 ,178

Within Groups 174659,750 24 7277,490

Total 257107,315 31

Lampiran 6. Hasil Analisis SPSS 20untuk kadar Hemoglobin

ANOVA

Lampiran 7. Perhitungan dosis pada tikus (Konversi dosis mencit 20 g ke

tikus 200 g)

No Perlakuan Konversi mencit ke tikus Dosis Tikus

1 Dosis CCl4 0,007 ---7,0 0,007 x 7 = 0,049 mL

2 Dosis Pelarut Minyak kelapa 0,007 ---7,0 0,1 x 7 = 0,7 mL

3 Dosis Larutan CCl4 +

Minyak kelapa

larutan CCl4 0,049 mL

dilarutkan dalam 0,7 mL larutan minyak kelapa

larutan CCl4 4,9 mL dilarutkan

dalam 70 mL minyak kelapa dan dosis CCl4 yang diberikan

Lampiran 8. Dosis Ekstrak metanol suren

30 gr dalam 100 mL akuades =0,03 mg dalam 1mL air

Lampiran 9. Tabel Konversi Perhitungan Dosis Untuk Berbagai Jenis (Spesies)

Hewan Uji Menurut Laurence & Bacharah, 1984

Lampiran 10

.

Pembuatan Ekstrak metanol daun suren

dicuci

dikering anginkan selama 3 hari

dipotong kecil-kecil dan diblender

dimasukkan kedalam botol tertutup

direndam dengan metanol

dibiarkan selama dua hari

disaring dengan menggunakan kertas

saring

diuapkan diatas water bath

dilarutkan dengan etil asetat sampai uji

flavonoid negatif (FeCl

3

)

diuapkan sampai kering

dilarutkan dengan metanol

ekstraksi dengan n-heksan

diuapkan sampai kering

Daun Suren

Bubuk daun suren

ekstrak kental metanol suren

Fraksi Etil asetat

Residu (diduga tanin)

Larutan metanol

n-heksan

Larutan

metanol

Lampiran 11

3.1.Pengambilan darah

Pengambilan darah tikus

Hati Tikus

3.2 Pengukuran Kadar SGPT dan SGOT

Darah EDTA

Mengukur kadar SGPT

3.3 Kadar Hemoglobin dan Penghitungan Jumlah Eritrosit

Darah dalam Cyanmethglobin

3.4.Pembuatan Ekstrak Suren dan minyak kelapa

Perendaman Serbuk suren

Suren pada rotary evaporator

pada metanol