ANALISIS KOMPONEN KIMIA MINYAK ATSIRI KULIT JERUK TELUR BUAYA (Citrus medica L.) SECARA GC-MS DAN UJI
ANTIOKSIDAN DENGAN METODE DPPH
ABSTRAK
Minyak atsiri kulit jeruk telur buaya(Citrus medica L) telah diisolasi dengan metode hidrodestilasi menggunakan alat Sthal. Kulit jeruk telur buaya dihidrodestilasi selama ±4-5 jam menghasilkan minyak atsiri 0,3064% (b/b). Komponen kimia minyak atsiri kulit jeruk telur buaya dianalisis menggunakan GC-MS menunjukkan ada 14 senyawa dimana komponen utamanya adalah 1-limonene (70,46%). Uji aktivitas antioksidan dilakukan dengan metode DPPH (2,2-diphenyl-1-picryl-hydrazil).Nilai IC50 yaitu 873,39g/L.
Kata Kunci : Jeruk Telur Buaya (Citrus medica L.) ,hidrodestilasi stahl, DPPH, limonen
ANALYSIS OF THE RIND OF JERUK TELUR BUAYA (Citrus medica L.) ESSENTIAL OIL COMPONENTS WITH GC-MS
AND TEST DPPH ANTIOXIDANT METHOD
ABSTRACT
Essential oils of the rind of jeruk telur buaya (Citrus medica L) has been isolated with hydrodestillation method using Stahl. It hydrodestilled for ± 4-5 hours to produce essential oil 0.3064% (w/w). The chemical components of essential oil of rind of jeruk telur buaya were analyzed by GC-MS showed there are 14 compounds in which the main components are 1-limonene (70,46%). Antioxidant activity test has been done with DPPH (2,2-diphenyl-1-picryl-hydrazil) method with IC50 values 873.39 mg/L.
Keywords: Jeruk Telur Buaya (Citrus medica L.), hydrodestillation stahl, DPPH, limonene