مﺎﮕﻨﻫ ﻪﺑ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ فﺮﺼﻣ ﺮﺛا حﻮﻄﺳ ﺮﺑ ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ8
ﻪﯾر ﺖﻓﺎﺑMDA
شﻮﻣ رﺎﺘﺴﯾو ﺮﻧ ﯽﯾاﺮﺤﺻ يﺎﻫ
يﺮﯿﻣا هرﺎﻬﺑ ﯽﻘﯿﻓﻮﺗ ﺮﻐﺻا ،1
يزﺮُﮔ ﯽﻠﻋ ،2
3
.1 ﻪﯿﻣورا هﺎﮕﺸﻧاد ,ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ ﺪﺷرا ﯽﺳﺎﻨﺷرﺎﮐ يﻮﺠﺸﻧاد
.2 ﺖﯿﺑﺮﺗ هﺪﮑﺸﻧاد رﺎﯿﺸﻧاد ﻪﯿﻣورا هﺎﮕﺸﻧاد،ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ
.3 نﺎﺠﻧز هﺎﮕﺸﻧاد ,ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ هوﺮﮔ رﺎﯾدﺎﺘﺳا
هﺪﯿﮑﭼ فﺪﻫ و ﻪﻨﯿﻣز ﯽﻣ نارﺎﮑﺷزرو رد ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ﺐﺟﻮﻣ ﺪﯾﺪﺷ ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ :
ﺶﻫوﮋﭘ زا فﺪﻫ .دﻮﺷ
نﻮﻟﺎﻣ ناﺰﯿﻣ ﺮﺑ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ فﺮﺼﻣ ﺮﺛا ﯽﺳرﺮﺑ ﺮﺿﺎﺣ يد
) ﺪﯿﻫﺪﻟآ ﻪﯾر ﺖﻓﺎﺑ رد (MDA
شﻮﻣ ﯽﯾاﺮﺤﺻ يﺎﻫ
ﯽﻃ رﺎﺘﺴﯾو ﺮﻧ .دﻮﺑ ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ 8
شور و داﻮﻣ ﺎﻫ
: نزو)رﺎﺘﺴﯾو ﺮﻧ ﯽﯾاﺮﺤﺻ شﻮﻣ ﺮﺳ 24
19.99 255.62±
ﻦﺳ و ) لﺮﺘﻨﮐ هوﺮﮔ ﻪﺳ ﻪﺑ ﯽﻓدﺎﺼﺗ رﻮﻃ ﻪﺑ يزﺎﺳﺎﻨﺷآ ﻪﺘﻔﻫ ﮏﯾ زا ﺲﭘ (ﻪﺘﻔﻫ8
=6 ,(n
) ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ
=9 ) ﻦﯿﻣﻮﮐرﻮﮐ +ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ,(n
=9 ﺮﻤﺗ .ﺪﻧﺪﺷ ﻢﯿﺴﻘﺗ (n
) ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾ ,ﻪﺘﻔﻫ8
ﻪﺴﻠﺟ5
ﻪﺑ ﺶﻫوﮋﭘ لوا ﮥﺘﻔﻫ رد نﺪﯾود تﺪﻣ و ﺖﻋﺮﺳ .ﺪﺷ مﺎﺠﻧا نﺎﮔﺪﻧﻮﺟ صﻮﺼﺨﻣ نادﺮﮔراﻮﻧ يور ﺮﺑ (ﻪﺘﻔﻫ رد ﺐﯿﺗﺮﺗ و ﻪﻘﯿﻗد رد ﺮﺘﻣ10
ﺖﻋﺮﺳ ﻪﺑ ﺮﺧآ ﮥﺘﻔﻫ رد و دﻮﺑ ﻪﻘﯿﻗد30 نﺎﻣز و ﻪﻘﯿﻗد رد ﺮﺘﻣ35
.ﺪﯿﺳر ﻪﻘﯿﻗد70
شﻮﻣ ﺰﺗ ﻪﻠﯿﺳو ﻪﺑ ار ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ ﯽﯾاﺮﺤﺻ يﺎﻫ ) ﺪﻧدﺮﮐ ﺖﻓﺎﯾرد ﯽﻗﺎﻔﺻ ﻞﺧاد ﻖﯾر
,ﻪﺘﻔﻫ8 رد ﻪﺴﻠﺟ 3
,ﻪﺘﻔﻫ ﯽﻠﯿﻣ 30
ﻢﯾﺰﻧآ حﻮﻄﺳ .(نﺪﺑ نزو زا مﺮﮔﻮﻠﯿﮐ ﺮﻫ يازا ﻪﺑ مﺮﮔ شور هدﺎﻔﺘﺳا ﺎﺑ ﻪﯾر ﺖﻓﺎﺑ رد MDA
هداد .ﺪﺷ هﺪﯿﺠﻨﺳ يﺮﺘﻣﻮﺘﻓﻮﺘﮑﭙﺳا ﻪﺑ ﺎﻫ
.ﺖﻓﺮﮔ راﺮﻗ ﻞﯿﻠﺤﺗ و ﻪﯾﺰﺠﺗ درﻮﻣ ﺲﻧﺎﯾراو ﻞﯿﻠﺤﺗ ﮥﻠﯿﺳو ﻪﺘﻓﺎﯾ
ﺎﻫ ﺞﯾﺎﺘﻧ :
وﮋﭘ ﻦﯾا زا ﻞﺻﺎﺣ ﻢﯾﺰﻧآ حﻮﻄﺳ ﺪﯾﺪﺷ ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﻪﮐ داد نﺎﺸﻧ ﺶﻫ
ﯽﻣ ﺶﯾاﺰﻓا ار ﻪﯾر ﺖﻓﺎﺑرد MDA
-
)ﺪﻫد 0.044
= (p
ﯽﻟﺎﺣرد . ﯽﻣ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ ﻪﮐ
)ﺪﻨﮐ يﺮﯿﮔﻮﻠﺟ ﺶﯾاﺰﻓا ﻦﯾا زا ﺪﻧاﻮﺗ 0.009
= .( p
ﻪﺠﯿﺘﻧ -
يﺮﯿﮔ ﻪﺘﻓﺎﯾ ﻦﯾا سﺎﺳا ﺮﺑ : ﯽﻣ ﺎﻫ
ﺪﯾﺪﺷ ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ زا ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ ﻪﮐ ﺖﻔﮔ ناﻮﺗ
ﯽﻣ يﺮﯿﮔﻮﻠﺟ .ﺪﻨﮐ
هژاو :يﺪﯿﻠﮐ يﺎﻫ ﯽﺘﻧآ ﺖﯿﻓﺮﻇ ,ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ ,ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ
.ﻪﯾر ﺖﻓﺎﺑ ,ﯽﻧاﺪﯿﺴﮐا
ﻪﻣﺪﻘﻣ
ﯽﻓﺮﺼﻣ نﮋﯿﺴﮐا ﺶﯾاﺰﻓا ﺐﺟﻮﻣ ,ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﯽﻃ رد يژﺮﻧا ﻪﺑ زﺎﯿﻧ رد
ﺖﻓﺎﺑ ﻖﯾﺮﻃ ﻦﯾا زا و هﺪﺷ لﺎﻌﻓ يﺎﻫ
ﻪﻧﻮﮔ ﺪﯿﻟﻮﺗ ﺶﻨﮐاو يﺎﻫ
رد ,ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا نﮋﯿﺴﮐا ﺮﯾﺬﭘ ﺴﮐاﺮﭘ ﺖﯾﺎﻬﻧ
و ﻦﯿﺌﺗوﺮﭘ ﯽﺸﯾﺎﺴﮐا تاﺮﯿﯿﻐﺗ و ﺪﯿﭙﯿﻟ نﻮﯿﺳاﺪﯿ
ﯽﻣ خرDNA
ﺖﺳا ﯽﻟﻮﻠﺳ ﺐﯿﺳآ يﺎﻨﻌﻣ ﻪﺑ ﻪﮐ ﺪﻫد )
(. 1 ﻂﺳﻮﺗ لﻮﻤﻌﻣ رﻮﻃ ﻪﺑ ﻪﮐ ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ يﻻﺎﺑ ﻢﺠﺣ
ﯽﻣ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ ﻪﺒﺨﻧ نارﺎﮑﺷزرو ﯽﻣ ,دﺮﯿﮔ
ﯽﺘﻧآ هﺎﮕﺘﺳد ﯽﯾارﺎﮐ ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﺪﻧاﻮﺗ ﺶﯾاﺰﻓا ,ﯽﻧاﺪﯿﺴﮐا
لﺎﮑﯾدار ﺪﯿﻟﻮﺗ و دازآ يﺎﻫ
دﻮﺷ ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ﺖﯾﺎﻬﻧ رد )
(.2 ياﺪﺘﺑا رد ﯽﻌﺿﻮﻣ نﻮﺧ نﺎﯾﺮﺟ ﺶﻫﺎﮐ ﺪﻨﯾآﺮﻓ
ﯽﺷزرو ﻪﯾﺬﻐﺗ و يژﻮﻟﻮﯾﺰﯿﻓ
ﺪﺷ ﺖﯿﻟﺎﻌﻓ ماﺪﻧا رد ﺪﯾﺪﺷ ﯽﻧﺪﺑ يﺎﻫ ﺖﯿﻟﺎﻌﻓ ياﺪﺘﺑا رد ﻪﮐ زﺎﯿﻧ درﻮﻣ ﯽﺘﻓﺎﺑ نﻮﺧ نﺎﯾﺮﺟ دﺪﺠﻣ يراﺮﻗﺮﺑ ﺲﭙﺳ و ﺪﯾ
ﺪﯿﭙﯿﻟ نﻮﯿﺳاﺪﯿﺴﮐاﺮﭘ ﺶﯾاﺰﻓا ﺪﻧور رد ﯽﻤﻬﻣ ﻞﻣﺎﻋ ناﻮﻨﻋ ﻪﺑ ،ﺪﻫﺪﯿﻣ يور هﺮﯿﻏ و لﺎﺤﻃ ،ﻪﯾر ،ﻪﯿﻠﮐ ﺪﻨﻧﺎﻣ ﯽﯾﺎﻫ ).دﻮﺷ ﯽﻣ بﻮﺴﺤﻣ نﻮﯿﺳاﺪﯿﺴﮐاﺮﭘ ﻪﯾﻮﻧﺎﺛ تﻻﻮﺼﺤﻣ زا ﯽﮑﯾ ﺪﯿﻫﺪﻟآ يد نﻮﻟﺎﻣ (3
دﺎﺠﯾا ﺚﻋﺎﺑ ﻪﮐ ﺖﺳا ﺪﯿﭙﯿﻟ
)دﻮﺷ ﯽﻣ ﯽﺘﻓﺎﺑ يﺎﻫ ﺐﯿﺳآ و ﻮﯿﺗاﺪﯿﺴﮐا سﺮﺘﺳا .(4
ﻢﻬﻣ ﯽﺘﺴﯾز ةدﺎﻣ ﻦﯾﺮﺗ دﻮﺟﻮﻣ
ﺖﺳا ﻦﯿﻣﻮﮐرﻮﮐ ﻪﺑﻮﭼدرز رد
دوﺪﺣ ﻪﮐ ﺎﺗ2
ﯽﻣ ﻞﯿﮑﺸﺗ ار ﻪﺑﻮﭼدرز نزو ﺪﺻرد8 ﻢﻬﻣ .ﺪﻫد
تاﺮﺛا ﻦﯾﺮﺗ ﯽﺘﺧﺎﻨﺷ ﺖﺴﯾز
نآ ﯽﺘﻧآ ﺖﯿﺻﺎﺧ ، -
يرﺎﻤﯿﺑ نﺎﻣرد و ﯽﻧاﺪﯿﺴﮐا ﺖﯿﺳﺎﺴﺣ ،ﯽﺷراﻮﮔ يﺎﻫ
ﯾاز ﺮﻤﯾاﺰﻟآ ،ﯽ و
ﺖﺳا نﺎﻃﺮﺳ )
(.5
لﺎﮑﯾدار ﺐﯿﺳآ ﺚﻋﺎﺑ دازآ يﺎﻫ ﺖﻓﺎﺑ ﻪﺑ
نﺪﺑ يﺎﻫ ﯽﻣ
ﺪﻧﻮﺷ . عﻮﻗو زا يﺮﯿﮔﻮﻠﺟ رد ﻞﻣاﻮﻋ ﻦﯾا ندﺮﮐ فﺮﻃ ﺮﺑ اﺬﻟ
فﺎﺑ دﺮﮑﻠﻤﻋ دﻮﺒﻬﺑ ﺰﯿﻧ و ﯽﺸﯾﺎﺴﮐا ﻂﯾاﺮﺷ .ﺖﺳا ﺪﯿﻔﻣ ،ﻪﯾر ﺖﻓﺎﺑ ﻪﻠﻤﺟزا نﺪﺑ يﺎﻫ
ﻪﺑ ﺎﺗ دراد ﺪﺼﻗ ﺮﺿﺎﺣ ﺶﻫوﮋﭘ
ﻪﺑ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ فﺮﺼﻣ ﺎﯾآ ﻪﮐ ﺪﻫد ﺦﺳﺎﭘ لاﻮﺳ ﻦﯾا مﺎﮕﻨﻫ
ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ8 هﺪﻨﯾاﺰﻓ
ﯽﻣ ﺪﻧاﻮﺗ ﺐﺟﻮﻣ
) ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ﺺﺧﺎﺷ ﺶﻫﺎﮐ شﻮﻣ ﻪﯾر ﺖﻓﺎﺑ رد (MDA
دﻮﺷ ﯽﯾاﺮﺤﺻ يﺎﻫ
؟ﺮﯿﺧ ﺎﯾ
ﯽﺳﺎﻨﺷ شور
نزو) رﺎﺘﺴﯾو ﺮﻧ ﯽﯾاﺮﺤﺻ شﻮﻣ ﺮﺳ 24 17.06
± 255.79 ﻦﺳ و
ﺎﺑ يزﺎﺳﺎﻨﺷآ ﻪﺘﻔﻫ ﮏﯾ زا ﺲﭘ (ﻪﺘﻔﻫ 8
ﯽﯾﺎﻨﺷور ﻪﺑ ﯽﮑﯾرﺎﺗ ﻪﺧﺮﭼ) رﻮﻧ ةﺪﺷ لﺮﺘﻨﮐ ﻂﯾاﺮﺷ رد تﺎﻨﯾﺮﻤﺗ 12:12
) ﺎﻣد ،(
/4 1
± ﺖﺑﻮﻃر و (داﺮﮔ ﯽﺘﻧﺎﺳ22
) 5
± ﻪﺑ ﯽﻓدﺎﺼﺗ رﻮﻃ ﻪﺑ ،تﺎﻧاﻮﯿﺣ هﺎﮕﺸﯾﺎﻣزآ (ﺪﺻرد45 )لﺮﺘﻨﮐ هوﺮﮔ3
=6 )ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ,(n
=9 ﻦﯾﺮﻤﺗ و (n
) ﻦﯿﻣﻮﮐرﻮﮐ +ﯽﺘﻣﺎﻘﺘﺳا
=9 .ﺪﻧﺪﺷ ﻢﯿﺴﻘﺗ (n ) ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ
,ﻪﺘﻔﻫ 8 نادﺮﮔراﻮﻧ يور ﺮﺑ (ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ 5
ﺐﯿﺗﺮﺗ ﻪﺑ ﺶﻫوﮋﭘ لوا ﮥﺘﻔﻫ رد نﺪﯾود تﺪﻣ و ﺖﻋﺮﺳ .ﺪﺷ مﺎﺠﻧا نﺎﮔﺪﻧﻮﺟ صﻮﺼﺨﻣ تﺪﻣ ﻪﺑ ﻪﻘﯿﻗد رد ﺮﺘﻣ10
ﺖﻋﺮﺳ ﻪﺑ ﺮﺧآ ﮥﺘﻔﻫ رد و دﻮﺑ ﻪﻘﯿﻗد30 لدﺎﻌﻣ) ﻪﻘﯿﻗد رد ﺮﺘﻣ35
ﺎﺗ80 و (ﻪﻨﯿﺸﯿﺑ ﯽﻓﺮﺼﻣ نﮋﯿﺴﮐا ﺪﺻرد85
نﺎﻣز ﻘﯿﻗد70
شﻮﻣ .ﺪﯿﺳر ﻪ ﺖﮐﺮﺷ زا هﺪﺷ ﻪﯿﻬﺗ) ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ ﯽﯾاﺮﺤﺻ يﺎﻫ
Merck
ﻪﻠﯿﺳو ﻪﺑ ار (نﺎﻤﻟآ
) ﺪﻧدﺮﮐ ﺖﻓﺎﯾرد ﯽﻗﺎﻔﺻ ﻞﺧاد ﻖﯾرﺰﺗ ,ﻪﺘﻔﻫ8
,ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ3 ﯽﻠﯿﻣ30
.(نﺪﺑ نزو زا مﺮﮔﻮﻠﯿﮐ ﺮﻫ يازا ﻪﺑ مﺮﮔ
هوﺮﮔ مﺎﻤﺗ ﻦﯾﺮﻤﺗ ﻪﺴﻠﺟ ﻦﯾﺮﺧآ زا ﺲﭘ ﺖﻋﺎﺳ48 ﻦﯾزﻼﯾاز و ﻦﯿﻣﺎﺘﮐ ﯽﺷﻮﻬﯿﺑ يوراد ﺎﺑ ﺎﻫ
ﺢﯾﺮﺸﺗ و شﻮﻬﯿﺑ
ﻢﯾﺰﻧآ حﻮﻄﺳ ﺲﭙﺳ .ﺪﺷ ﺖﺷادﺮﺑ ﺎﻬﻧآ ﻪﯾر ﺖﻓﺎﺑ و ﺪﻧﺪﺷ يﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا شور زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﯾر ﺖﻓﺎﺑMDA
جﻮﻣ لﻮﻃ رد يرﻮﻧ بﺬﺟ) شور ،ﺮﺘﻣﻮﻧﺎﻧ532
TBARS
)ﺖﻓﺮﮔ راﺮﻗ ﺶﺠﻨﺳ درﻮﻣ ( هداد .(6
ﻪﺑ ﺎﻫ ﻞﯿﻠﺤﺗ ﮥﻠﯿﺳو
)ﺪﺷ ﻞﯿﻠﺤﺗ و ﻪﯾﺰﺠﺗ ﻪﻓﺮﻃ ﮏﯾ ﺲﻧﺎﯾراو 0.05
<
(p
.
ﻪﺘﻓﺎﯾ ﺎﻫ
يﺮﯿﮔ ﻪﺠﯿﺘﻧ و ﺚﺤﺑ
ﻢﯾﺰﻧآ ﺢﻄﺳ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد ﯽﺘﻣﺎﻘﺘﺳا هوﺮﮔMDA
ﯽﻨﻌﻣ ﺶﯾاﺰﻓا ﻦﯿﻣﻮﮐرﻮﮐ+ﯽﺘﻣﺎﻘﺘﺳا و لﺮﺘﻨﮐ يﺎﻫ اﺪﯿﭘ يراد
ﻢﯾﺰﻧآ ﺢﻄﺳ ﻦﯿﻣﻮﮐرﻮﮐ+ﯽﺘﻣﺎﻘﺘﺳا هوﺮﮔ رد ,ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد .دﺮﮐ رﻮﻃ ﻪﺑ ﻪﯾر ﺖﻓﺎﺑMDA
ﯽﻨﻌﻣ ﻦﯿﯾﺎﭘ يراد ﯽﻣ ﯽﺗرﺎﺒﻋ ﻪﺑ .ﺖﺳا ﺮﺗ
ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ ياﺮﺟا مﺎﮕﻨﻫ ﻪﺑ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ فﺮﺼﻣ ﺖﻔﮔ ناﻮﺗ
ﯽﻣ ﺪﯾﺪﺷ ﻢﯾﺰﻧآ ﺢﻄﺳ ﺶﯾاﺰﻓا زا ﺪﻧاﻮﺗ
ﺳ .ﺪﯾﺎﻤﻧ يﺮﯿﮔﻮﻠﺟMDA
ﻢﯾﺰﻧآ حﻮﻄ ﻦﯿﻣﻮﮐرﻮﮐ+ﯽﺘﻣﺎﻘﺘﺳا هوﺮﮔMDA
ﯽﻨﻌﻣ توﺎﻔﺗ لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﯽﻣ نﺎﺸﻧ و ﺪﻧراﺪﻧ ﻢﻫ ﺎﺑ يراد
ﺐﺟﻮﻣ ﺪﻧاﻮﺗ ﯽﻣ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ فﺮﺼﻣ ﻪﮐ ﺪﻫد
نﻮﻟﺎﻣ ﯽﻧاﺪﯿﺴﮐا ﺺﺧﺎﺷ حﻮﻄﺳ ﺶﯾاﺰﻓا زا يﺮﯿﮔﻮﻠﺟ يد
ﺐﯿﺳآ ﻪﺠﯿﺘﻧ رد و دﻮﺷ ﺪﯿﻫﺪﻟآ ﺖﯿﻟﺎﻌﻓ زا ﯽﺷﺎﻧ يﺎﻫ
لﺎﮑﯾدار ﯽﺘﻧآ و دازآ يﺎﻫ ناﺪﯿﺴﮐا
ﺎﻫ ﺪﻫد ﺶﻫﺎﮐ ار .
ﯽﯾاﺮﺟا مﺎﯿﭘ -
ﺶﻫوﮋﭘ ﯽﻤﻠﻋ
لﺎﮑﯾدار ﺶﯾاﺰﻓا زوﺮﺑ زا يﺮﯿﮕﺸﯿﭘ ﺚﻋﺎﺑ ﻦﯿﻣﻮﮐرﻮﮐ ﻞﻤﮑﻣ فﺮﺼﻣ ﺶﻫوﮋﭘ ﻦﯾا رد رﺎﺸﻓ و ﺪﺷ دازآ يﺎﻫ
ﻪﻓﺮﺣ نارﺎﮑﺷزرو ﻪﺑ ﻦﯾاﺮﺑﺎﻨﺑ .داد ﺶﻫﺎﮐ ار تﺎﻨﯾﺮﻤﺗ زا ﯽﺷﺎﻧ ﯽﺸﯾﺎﺴﮐا ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ مﺎﺠﻧا ﻪﺑ رﻮﺒﺠﻣ ﻪﮐ يا
ﯽﻣ ﻪﯿﺻﻮﺗ ﺪﻨﺘﺴﻫ ﺪﯾﺪﺷ ﺮﺑ رد دﻮﺷ
.ﺪﻨﻨﮐ هدﺎﻔﺘﺳا (ﻦﯿﻣﻮﮐرﻮﮐ يوﺎﺣ) ﻪﺑﻮﭼدرز ﯽﯾاﺬﻏ ﮥﯾودا زا دﻮﺧ ﯽﯾاﺬﻏ ﮥﻣﺎﻧ
ﻊﺑﺎﻨﻣ 1. Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas M, Lopez F, Abellan P. The effects of an antioxidant- supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists.European journal of applied physiology. 2005;95(5-6):543-9
2. Teixeira V, Valente H, Casal S, Marques F, Moreira P. Antioxidant status, oxidative stress, and damage in elite trained kayakers and canoeists and sedentary controls. Int J Sport NutrExercMetab.
2009;19(5):443-56.
3. Chevion S, Moran D, Heled Y. Plasmaantioxidant status and cell injury after severe physicalExercise.
PNAS; 2003. 100(9): 5119-23.
4. Tauler P, Sureda A, Cases N, et al. Increased lymphocyte antioxidant defences in response to exhaustive exercise do not prevent oxidative damage. J Nutr Biochem 2006; 17(10): 665-671.
5. Prasad S, Tyagi AK, Aggarwal BB. Recent Developments in Delivery, Bioavailability, Absorption and Metabolism of Curcumin: the Golden Pigment from Golden Spice. Cancer Research and Treatment.
2014;46(1):2-18.
6. Kaya H, Sezik M, Ozkaya O, et al: Lipid peroxidation at various estradiol concentrations in human circulation during ovarian stimulation with exogenous gonadotropins. Horm Metab Res , 2004 :36:693.
2.95
3.38
2.82
0 0.5 1 1.5 2 2.5 3 3.5 4
لرﺗﻧﮐ ﯽﺗﻣﺎﻘﺗﺳا ﯽﺗﻣﺎﻘﺗﺳا+نﯾﻣوﮐروﮐ
حوطﺳ نﯾﮕﻧﺎﯾﻣMDA ﮫﯾر)U/mg.pro(
*
حﻮﻄﺳ ﻦﯿﮕﻧﺎﯿﻣ ﻪﯾرMDA
) U/mg.pro
* ( ﯽﻨﻌﻣ توﺎﻔﺗ = ﯽﺘﻣﺎﻘﺘﺳا+ﻦﯿﻣﻮﮐرﻮﮐ و لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ راد
) 0.05
<
.(p
The effect of curcumin supplementation during 8- weeks of endurance training on tissue of lung MDA level of male wistar rat
Amiri.Bahareh1, Tofighi.Asghar2, Gorzi.Ali3 1. MSc in Exercise Physiology, University of urmia
2. Associate Professor, University of urmia 3. Assistant Professor, University of zanjan
Abstract
Aim and Background: High volume endurance training, can reduce system performance antioxidant, increased production of free radicals and oxidative stress in the end. The purpose of this study was to investigate the effects of curcumin supplementation on tissue of lung malondialdehyde (MDA) as oxidative status level during 8 weeks of endurance training in male wistar rats. Materials and Methods: 24 Male wistar rats (weight=
255.79±17.06 g, aged= 8 weeks) after one week familiarization period, were randomly divided in to 3 groups;
Control (n=6), Endurance (n=9) and Endurance+curcumin (n=9).Endurance training (8 weeks, 5 sessions a week) carried out on animal treadmill. The speed and duration of running were 10 m/min and 30 min at first week, respectively and reached to the 35 m/min and 70 min at last week. The animals in Endurance+curcumin group received curcumin supplement by subperitoneal injection (8 weeks, 3 sessions a week, 30 mg/kg.Bw).
MDA levels of lung tissues were measured by spectrophotometric (absorbance at 532 nm, using TBARS method). Analysis of variance was used to analysis of the data. Results: The results of this study showed that strenuous endurance training increases the tissue of lung MDA level (p<0.05, p<0.009) and curcumin supplementation can prevent from increasing the MDA level of lung tissue(p<0.05, p<0.044). . Posts enzyme MDA in the control group 2.93±0.48, in strength 3.38±0.25 and endurance+Curcumin 2.82 ± 0.45, respectively.
Conclusion: Based on the findings of this study, curcumin supplementation can prevent from oxidative stress during strenuous endurance training.
Keywords: Endurance training, Curcumin supplementation, Anti-oxidant capacity, tissue of lung.
