Nghien cifu YDHCT Viet Nam

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Nghien cifu YDHCT Viet Nam

\ N G H I E N ClTU D O C T I N H C A P VA A N H Hl/CnVG C U A V I E N N A N G ClTNG D I E L S I N L E N C A C C H l S O H U Y E T H O C T R E N T H O T H l T C N G H I E M

A c u t e toxicity a n d influence on hematological index of Dielsin on e x p e r i m e n t a l a n i m a l s

*Nguyen Quoc Huy ', ** Nguyen Trpng Thong Bai hpc Duac Hd Npi, ^ Trudng Dgi hpc Y Hd Npi Tom t3t

Nghien cihi dpc tinh cdp tren chupt nhdt trdng vd dnh huong cua vien nang Dielsin len cdc chi sd huyet hpc tren thd thuc nghiem duac thuc hien nhdm 2 muc tieu: 1. Xdc dinh dpc tinh cdp vd Iiiu chit 50% cua vien nang ciing Dielsin tren chupt nhdt trdng. 2. Nghien cihi dnh hudng cua vien nang cimg Dielsin len cdc chi sd huyit hpc tren tho th\ec nghiim. Kit qud: vien nang Dielsin khong co dpc tinh cdp vd chua xdc djnh duac LDs„ tren chupt nhdl trdng theo dudng udng. Sau 8 tudn. thd dupe udng vien nang Dielsin vdi lieu 0.18 g duac lieu/ kg /ngdy vd vdi Iiiu 0,54 g duac Iiiu /kg /ngdy (gdp 3 Idn lieu 1) khong thdy dnh hudng den the trgng, chiic nang tao mdu cua tho.

Tir kh6a: Vien nang Dielsin, Cii Dom, Stephaoia dielsiana Y. C. Wu, d$c tinh c^p, anh hirorng tren huyet hgc, thyc nghidm.

Summary

A C U T E T O X I C I T Y AND I N F L U E N C E ON H E M A T O L O G I C A L I N D E X O F D I E L S I N C A P S U L E ON E X P E R I M E N T A L A N I M A L S OJectives: (l)To identify the acute toxicity and to evaluate the LD 50 of Dielsin capsule on mice: (2) to evaluate the influence of Dielsin capsule on hematological examinations of experimental rabbits. Results and Conclusion • we have not yet identified acute toxicity and the LD)o of Dielsin capsule. After 8 weeks using Dielsin capsule at dose ofO.lSg /kg bodyweighl per day and 0.54g g/kg bodyweighl per day (higher than 3 times of dose of group 1). there were not any abnormal sign of general state, growth and hematological examinations on experimental rabbits.

Keywords: Dielsin capsule, Stephania dielsiana Y. C. Wu, acute toxicity, hematological index, experiment.

Dja chi lien h$: Nguyin Quoc Huy - Trucmg Dai hoc Du<7C Ha Noi NgSy nhan bai: 08/10/2014 Dja chi email: [email protected] Ng^y phan bi^n: 20/10/2014 Phan bi?n khoa h^c: PGS.TS. Tr4n Qu6c Binh Ngay dSng bao: 19/12/2014

•TS;** PGS.TS

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Journal of research In viet nam traditional medicine and pharmacy No 43 - 2014 I. DAT VAN DE

Qua dieu tra tri thirc sii dung ciia nguoi din cac nha khoa hpc va cac thiy lang (dan toe Dao) 6 khu vuc vung dem xung quanh vuon quoc gia Ba Vi Ha Npi, da tim ra loai cii dom la mpt trong nhiing cay thuoc quy ciia nguoi dan trong khu vuc vai tac dung tru phong thap, dieu tri than kinh tpa, dau da day, cac benh nhin buoc dau da co ket qua kha t6t. Da co nhieu nghien ciru ve thuc vat, giam dinh ten khoa hpc ciia cil dom la Stephania dielsiana Y. C. Wu va phin lap nhieu chit tinh khiet nhu: rotundin, oxostephanin, dehydrocrebanin,..[l]. Trong qua trinh nghien ciiu chS phim Dielsin co nguon g6c tir phan doan diclomethan va ethylacetat (a pH:4-5) ciia dich chiet ethanol cua cu dom, viec dinh gia tinh an loan ciia che phim trong giai doan tidn lam sing la viec lam rit can thiet. Vi vay, nghien cuu nay dugc tien hanh nham 2 muc tieu:

- Xdc dinh dgc tmh cap vd lieu chit 50% cua vien nang cung Dielsin tren chupt nhdt trang.

Nghien cieu dnh hie&ng ciia vien nang cirng Dielsin len cdc chi sd huyet hgc tren tho thirc nghiem.

II. CHAT LIEU, DOI TLTONG VA PHUONG PHAP NGHIEN CtTU

2.1. Chat lieu nghien cmi

Chit lieu la vien nang ciing Dielsin co ngudn gdc tir phin doan diclomethan va ethylacetat (o pH:4-5) tir dich chiet ethanol cua cii dom de sin xuit vien nang ciing (500 mg).

2.2. Doi tvong nghien cihi Nghien ciru dpc tinh cip: Chupt nhat trang chiing Swiss, ca 2 giong, khoe manh, trpng lupng 18 - 22g do Vien Ve sinh dich te Trung uang cung cap.

Nghien cihi anh huong cua vien nang ciing Dielsin len chi sd huyet hpc: Tho chung Newzealand White, ldng trang, trpng lugng 1,8-2,2 kg do Trung tim chan nudi va cflng cip dpng vit thi nghiem Dan Phugng, Ha Npi cung cap.

2.3. May moc va hoa chat phuc vu nghien cthi

- Dpc tinh cip: Cin di^n tii ciia Nhit, dp chinh xac 0,001 gam, kim diu til cho chupt udng, cdc chia vach, bom kim tiem 1ml.

- Anh huong ciia Cii Dom len chi sd huydt hpc: Dung dich xet nghiem mau ABX Minidil LMG ciia hang ABX - Diagnostics, dinh lugng tren may Vet abc^^ Animal Blood Counter.

2.4. Phvovg phap nghien ciru

* Chiet xudt duoc lieu vd bdo che vien nang cung Dielsin:

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Nghien cicu YDHCT Viet Nam 36 43 - 2014 Dirgtc lieu dinjrc riia sach, cao vo,

thai lat mong, sky kho a eo'C va xay thanh bpt tho dat tieu chu4n Dugc diSn Viet Nam IV

Chiet dugc lieu bang dung moi ethanol tuyet doi : acid acetic 5% (3:2) pH 4-5 bang phu-cmg phap ngSm kiet.

Dich chiet dugc cdt thu hoi dung m6i.

Dich chiet thu dugc tiep tuc dugc chiSt l4n lugt voi diclomethan, ethylacetat.

Cat thu hoi dimg moi. Gop can phan doan diclomethan va ethylacetat dugc phan doan 1 (PDl) dl dem thii tac dung. Lieu dung trong nghien cuu la lieu tinh theoiugng com cua vien nang 500mg.

TIT cao dac, nghien cuu cong thuc bao che voi cao tren thu dugc com vien nang Dielsin.

STT 1 2 3 4 5 6 7 8 9

Thanh phan Cao kho dugc lieu (PDl) MgC03

Avicel Tinh hot PVP Nipagin Nipazol Magnesium stearat Talc

So lu'ffng 320

48 96 32 0,127

0,95 0,095

2,5 2,5

Dtfn vi mg mg mg mg mg mg mg mg mg

Tieu chuan TCCS DDVNIV DDVNIV DDVNIV DDVNIV DDVNIV DDVNIV DDVNIV DDVNIV Mdi vien nang Dielsin co 500 mg

cdm.

* Nghien cuu doc tinh cdp cua vien nang Dielsin theo ducmg uong tren chuot nhdt trdng:

- Phuang phap tiSn hanh: Phuang phap Litchfield - Wilcoxon va theo huong dan cua WHO [4], [5].

- Tien hanh thi nghiem:

Chuan bi mau thii cho chupt udng:

Li^u diing tren nguoi 2-3 vien, 2-3

lin/ngay. Nhu vay lieu tren nguai tinh theo kg the trpng tir: 0,04 g cdm - 0,09 g cdm/kg/ngay (ciia vien nang Dielsin, tinh tren nguoi Ion truong thanh nang 50kg).

Cin 64g cdm them vao 35 ml nuac cat, nghien ky trong cdi sir thu dugc 90 ml viia dii cao long dim dac nhat cd the cho chupt nhat trang udng dugc bang kim diu tu chuyen dung.

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Journal of research in viet nam traditional medicine and pharmacy Truac khi tien hanh thi nghiem, cho

chugt nhin an qua dem.

Chupt dugc chia thanh cac 16 khac nhau, moi 16 10 con. Cho chupt uong c6m cu Dom voi lieu tang din trong cung mpt thS tich de xac dinh h6u thap nhat gay chSt 100% chupt va lieu cao nhat kh6ng gay chet chupt (gay chSt 0% chupt).

Theo doi tinh trang chimg cua chupt, qua trinh dien bien bit d4u co dau hieu nhiem dpc (nhu n6n, co giat, kich dpng...) va s6 lugng chupt ch£t trong vong 72 gia sau khi u6ng thu6c.

Tat ca chupt chet dugc m6 de danh gia t6n thuang dai the.Tir do xay dung d6 thi taySn tinh dS xac dinh LD50 cua thu6c thii. Sau do tiip tuc theo doi tinh trang ciia chupt den het ngay thii 7 sau khi u6ng thu6c.

* Nghien cmi anh hic&ng cim vien nang Dielsin ten cdc chi so huyit hoc:

Phuong phap ti6n hanh: Theo huong din ciia WHO d6i vol dugc lieu va thu6c y hpc co truySn [5].

- Tien hanh thi nghiem:

Tho dugc chia lam 3 16, mSi 16 10 con, moi con nhot rieng mot chuong.

Lo chirng: uong nuoc cit 3ml/kg/ngay.

Lo tri 1: u6ng c6mcua vien nang Dielsinlieu 0,18 g/kg.

L6 tri 2: uong com cua vi8n nang Dielsin liSu 0,54g/kg (gip 3 ISn 16 tri 1).

Thd dugc udng nudc ho^c thudc thur trong 8 tuan.

- Cac chi tieu theo dot tnrac vd trong qud trinh nghien cmi:

+ Tinh trang chung, thi trpng ciia thd.

+ Danh gia chuc phan tao mau:

Thdng qua sd lugng hdng cau, thi tich trung binh hdng cau, ham lugng hemoglobin, hematocrit, sd lugng bach cau, cdng thuc bach cau va sd lugng tieu cau.

2.5. D|a diem va thM gian tien hanh: Nghien ciiu dugc tien hanh tai Labo Bd mdn Dugc ly truong Dai hpc Y Ha Ndi tu thing 6-11/2014

- Cac thdng sd theo doi dugc kiem tra vao trudrc liic udng thudc, sau 4 tuin udng thudc, va sau 8 tuan uong thudc.

2.6. Xu Iy so lieu

- Cic sd lieu nghien ciiu dugc xir ly thdng ke theo phuong phap t-test Student. Sd lieu dugc bieu diln duoi dang ; X±SD. Su khac biet cd y nghTa khi p < 0,05.

III. KET QUA NGHIEN ClTU 3.1. Doc tinh cap

Chupt nhat trlng dugc udng com ciia vien nang Dielsin tir lieu thip nhit ddn lilu cao nhit: 0,25 ml/lOg, 3 lin

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Nghien curu YDHCT Viet Nam trong 24 gio, mdi lin each nhau it nhit 2 gid. Ld chupt da udng den lieu 75ml/kg the trong chupt tuong duong 53,33g cdm/kg nhung khdng cd chupt nao chet, khdng xuit hien trieu chiing bit thuang nao trong 72 gid sau udng thudc lin diu va trong sudt 7 ngay.

Lieu 53,33 g cdm/kg la lilu tdi da cd the dimg dugc bang dudng udng de danh gia ddc tinh cap ciia vien nang Dielsin: (ndng dp dac nhit, the tich mdi !an udng tdi da, sd lin dung tdi da trong 24 gid). So sanh vdi lieu du kien tren ngudi: chupt nhat trang da udng Bang I. Anh hirong cua vien

den lilu gap 59 -133 lan (Tinh he sd ngoai suy tren chupt gap 10 lan lieu tren ngudi) nhung khdng xuit hien ddc tinh cap.

3.2. Tinh trang chung va cac chi so huyet hpc cua thd:

Tinh trgng chung: Trong thdi gian thi nghiem, thd a ci 3 Id hogt dpng binh thuang, nhanh nhen, mat sang, ldng mugt, an udng tdt, phan khd. Khdng thay bieu hien gi dac biet d ca 3 Id thd trong sudt thdi gian nghien ciiu.

Sir thay doi the trgng tho:

nang Dielsin den the trong tho

Thcri gian

Trirac uong thuoc Sau 4 tuan uong thuoc P truac - sau

Sau 8 tuin uong thuoc P trudc - sau

Ld chfrng (n=10) Tr^ng lu-yng (kg) X±SD 1,84 ±

0,14 2,29 ±

0,24

<0,05 2,37 ±

0,15

<0,05

% tSng tri)ng lirgTig

23,19±

9,18 25,15 ±

5,42

L6 tri 1 (n"IO) TrQng luyng (kg) X±SD 1,86 ±

0,18 2,42 ±

0,26

<0,05 2,51 ±

0,22

<0.05

% taog trgng l u ^ g

30,15 ± 6,44 35,57 ±

9,70

Lo tri 2 (n=IO) Trgng lirgTig (kg) X±SD 1,89 ± 0,19 2,31 ±

0,20

<0,05 2,36 ±

0,15

<0,05

% ting trgng lirgng

22,87 ± 8,22 26,22 ±

12,36 p (t-test Student)

>0,05

<0,05

Sau 4 tuhn va 8 tuan uong vien nang Dielsin, trpng lugng tho a ca 3 16 deu tang so vol truoe khi nghien ciiu. Kh6ng co su khac biet vi miic dp gia tang trpng lugng tho giiia 16 chiing va cac 16 dung thuoc thu (p>0,05), tham chi trpng lugng cua 16 u6ng vien nang Dielsin lieu thSp 0,18 g/kg con tang so voi 16 chiing sinh hpc (p<0,01).

- Ddnh gid chirc ndng tgo mdu:

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Journal of research in viet nam traditional medicine and pharmacy No 43 - 2014 Bang 2. Anh hirong cua vien nang Dielsin den so Iirgng hong cau

trong mau tho

Thoi gian Truoe uong thu6c

Sau 4 tuan uong thuoc p (truoe -

sau) Sau 8 tuan uong thuoc p (truoe -

sau)

So lugng hong cau ( T/I) Lo chirng (n=10)

X±SD 5,24 ± 0,34 5,50 ± 0,47

>0,05 5,56 ±0,51

>0,05

Lo tri 1 (n=10) X±SD 5,09 ± 0,44 5,30 ±0,54

>0,05 5,54 ± 0,57

>0,05

Lo tri 2 (n=10) X±SD 5,19 ±0,44 5,46 ± 0,25

>0,05 5,48 ± 0,40

>0,05

P (t- test Student)

>0,05

Sau 4 tuan va 8 tuin udng vien nang Dielsin, sd lugng hdng ciu d ca Id trj 1 (udng vien nang Dielsin lihu 0,18g/kg/ngay) va Id tri 2 (udng vien nang Dielsin liSu 0,54g/kg/ngay) deu khdng cd sir khac biet cd y nghia so vdi Id chiing (p > 0,05).

Bang 3. Anh huorng cua vien nang Dielsin den ham luong huyet s^c to trong mau thd

Thoi gian Tnroc u6ng thu6c Sau 4 tuan u6ng thu6c p (truac -

sau) Sau 8 tuan uong thuoc

p (truac - sau)

Ham lugng huyet sac to (g/dl) Lo chihig (n=10)

X±SD 11,07 ±0,54

10,44 ±1,79

>0,05 11,85 ±1,44

>0,05

Lo tri 1 (n=10) X±SD 11,24 ±0,96 10,02 ±3,26

>0,05 11,84 ±0,81

>0,05

Lo tri 2 (n=10) X±SD 11,18±0,90 10,24 ±1,08

>0,05 12,26 ± 1,38

>0,05

P (t- test Student)

>0,05

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Nghien ciiu YDHCT Viet Nam

Sau 4 tuan va 8 tuan uong vien nang Dielsin, ham lugng huyet sac to g ca 16 tri 1 (u6ng vien nang Dielsin hSu 0,18g/kg/ngay) va 16 tri 2 (u6ng vien nang Dielsin liSu 0,54g/kg/ngay) deu kh6ng co sir khac biet c6 y nghia so vol 16 chiing (p > 0,05).

Bang 4, Anh hirong cua vien nang Dielsin den hematocrit trong mau tho

Thoi gian

Truoe uong thuoc

Sau 4 tuan u6ng thu6c p (truoe -

sau) Sau 8 tuan uong thuoc p (truac -

sau)

Hematocrit (%) Lo chung (n=10)

X±SD 32,66 ±1,25 33,16 ±2,10

>0,05 35,53 ± 1,96

<0,05

Lo tri 1 (n=10) X±SD 32,54 ±3,15 34,24 ± 2,09

>0,05 35,46 ± 2,42

>0,05

Lo tri 2 (n=10) X±SD 33,17 ±2,82 35,03 ±1,89

>0,05 35,67 ± 3,75

>0,05

P (t- test Student)

>0,05

Sau 4 tuan va 8 tuin udng vien nang Dielsin, lugng hematocrit d ci Id tri 1 (udng vien nang Dielsin lidu 0,18g/kg/ngay) va Id tri 2 (udng vien nang Dielsin lidu 0,54g/kg/ngay) d8u khdng cd su khac biet cd y nghia so vdi Id chirng (p > 0,05).

BangS. Anh huong ciia vien nang Dielsin den the tich trung binh hong can trong mau thd

Thoi gian

Truac u6ng thu6c

Sau 4 tuan uong thuoc p (truac -

sau) Sau 8 tuan u6ng thu6c p (truac -

sau)

The tich trung binh hong cau ( fl ) Lo chung (n=10)

X±SD 61,90 ±3,11 62,80 ±3,01

>0,05 61,83 ±4,62

>0,05

Lo tri 1 (n=10) X±SD 64,10 ±3,78 65,00 ± 5,56

>0,05 64,71 ±3,73

>0,05

Lo tri 2 (n=10) X±SD 63,80 ±1,23 64,00 ±1,33

>0,05 65,00 ± 2,58

>0,05

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Journal of research in viet nam traditional medicine and pharmacy No 43 - 2014 Sau 4 tuSh va 8 tuSh uong vien nang Dieism, the tich trung buih hong ciu a ca 16 tri 1 (u6ng vien nang Dieism liSu 0,18g/kg/ngay) va 16 tri 2 (uong vien nang Dielsin lidu 0,54g/kg/ngay) ddu khong co su khac biet co y nghia so vai 16 chiing (p > 0,05).

Bang 6. Anh huong cua vien nang Dielsin den so lugng bach cau trong mau tho

Thoi gian

Truac u6ng thu6c

Sau 4 tuan u6ng thuoc p (truac -

sau) Sau 8 tuan u6ng thu6c p (truac -

sau)

So lugng bach cau (G/1) Lo chung (n=10)

X±SD 7,31 ±1,34 8,61 ±2,40

>0,05 9,70 ± 2,87

<0,05

Lo tri 1 (n=10) X±SD 7,57 ±1,67 9,72 ± 2,59

>0,05 9,81 ± 1,63

<0,05

Lo tri 2 (n=10) X±SD 7,70 ± 2,02 9,20 ±1,38

>0,05 9,97 ± 2,37

<0,05

>0,05

Sau 4 tuan va 8 tuan udng vien nang Dielsin, sd lugng bach cau d ca Id tri 1 (udng vien nang Dielsin lieu 0,18g/kg/ngay) va Id tri 2 (udng vien nang Dielsin lieu 0,54g/kg/ngay) deu khdng cd sir khac biet cd y nghia so vdi Id chiing (p > 0,05).

Bang 7. Anb hvdug cua vien nang Dielsin den cong thvc bach cau trong mau tho

Thoi gian

Truac u6ng thuoc Sau 4 tuan uong thuoc p (truac - sau)

Sau 8 tuan uong thu6c p (truac - sau)

Cdng thuc bach cau Lo chirng (n=10)

X±SD Lympho

(%)

79,10 ± 9,49 76,11 ±

8,92

>0,05 76,67 ±

7,20

>0,05 Trung tinh (%)

20,90 ± 9,49 23,89 ±

8,92

>0,05 23,33 ±

7,20

>0,05

Lo tri 1 (n=10) X±SD Lympho

(%)

74,90 ± 11,65 69,90 ±

12,44

>0,05 83,86 ±

5,08

<0,05 Trung tinh (%)

25,10 ± 11,65 30,10 ±

12,44

>0,05 16,14 ±

5,08

<0,05

Lo tri 2 (n=10) X±SD Lympho

(%)

79,30 ± 9,96 76,80 ±

10,30

>0,05 83,71 ±

5,82

<0,05

Trung tinh

(%)

20,70 ± 9,96 23,20 ±

10,30

>0,05 16,29 ±

5,82

<0,05

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Nghien cUu YDHCT Viet Nam

Sau 4 tuan va 8 tuin u6ng vien nang Dielsin, xet nghiem cong thiic bach ciu 6 ca 16 tri I (u6ng vien nang Dielsin lieu 0,18g/kg/ngay) va 16 tri 2 (u6ng vien nang Dielsin lieu 0,54g/kg/ngay) dSu khong co su khac biet co y nghia so vdi 16 chiing (p>0,05).

Bang 8. Anh huong cua vien nang Dielsin den so lugng tieu can trong mau tho

Thoi gian

Truoe u6ng thu6c

Sau 4 tuan u6ng thuoc p (truac -

sau) Sau 8 tuan u6ng thuoc p (truoe -

sau)

So lugng tieu can (G/l) Lo chung (n=10)

X±SD 407,80 ± 82,42

368,30 ± 57,06

>0,05

397,73 ± 84,36

._i.0,05

Lo tri 1 (n=10) X±SD 436,50 ± 99,04

352,90 ± 57,96

>0,05

337,43 ± 48,70

<0,05

Lo tri 2 (n=10) X±SD 432,80 ±63,14

370,90 ±92,60

>0,05

340,71 ± 94,40

>0,05

Sau 4 tuan va 8 tuin udng vien nang Dielsin, sd lugng tieu ciu d ci Id tri 1 (udng vien nang Dielsin lieu 0,18g/kg/ngay) va Id tri 2 (udng vien nang Dielsin lieu 0,54g/kg/ngay) deu khdng cd su khac biet cd y nghia so vdi Id chiing (p > 0,05).

IV. BAN LUAN:

4.1. Dpc tinh cap:

Chupt da uong vdi the tich tdi da la 0,25 ml/10 g chupt, 3 lin trong 24 gid. Day la lugng thudc tdi da ma chupt cd the dung nap dugc, vi khdng cd chupt chet nen chua xac dinh dugc LD50 ciia vien nang Dielsin tren chupt nhat trang bang dudng udng theo

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Journal of research in viet nam traditional medicine and pharmacy No 43 - 2014 phuang phap Litchfield - Wilcoxon.

Lidu tdi da chupt da udng la 75 ml/kg thd trpng chupt, tuong duong 490,10 g dugc lieu/kg, khdng cd chupt nao chet nen khdng xac dinh dugc lieu gay chet (lethal dose - LD) va lieu chet nam muoi phin tram (LD5o).Theo hudng din cua WHO, tat cac cac thudc cd ngudn gdc tu dugc lieu hay hoa chit deu phai danh gia ddc tinh cap va ban tnrdng dien tren siic vit trudc khi dua vao thii nghiem tren ngudi [5]. Cac nghien ciiu ve dugc ly cho thay chi Stephania tuong ddi an toan [3]. Vien nang Dielsinkhdng cd ddc tinh cip tren dudng udng 6 chupt nhat trang.

4.2. Anh hu-dmg cua vien nang Dielsin len tinh trang chung va cac chi so huyet hoc: Tinh trang chung, trpng lugng co the va cac chi sd huyet hpc la nhiing xet nghiem bat budc khi danh gia ddc tinh cua thudc thii [5].

Mau la mdt td chiic rit quan trpng vi mau lien quan mat thiet vdi mpi bd phin, CO quan trong co the.Ve mat benh ly, mau chiu anh hudng cua tit

ci cac td chiic dd nhung ddng thdi cung bi anh hudng va phan anh tinh trang rieng cua co quan tao mau [4] .Neu thudc cd anh hudng den ca quan tao mau thi trudc het cac thanh phan cua mau se bi thay ddi, dac bi?t thudng lam giam sd lugng bach cau [2], [5].Vi vay, cac xet nghiem ve so lugng hdng cau, sd lugng bach cau, cdng thiic bach cau, sd lugng tieu ciu ciia thd thi nghiem dugc xac dinh.

Cac chi sd tren ciia thd d ca hai Id tri deu thay ddi khdng cd y nghia so vdi trudc khi dung thudc (test trudc - sau, p >0,05) va so vdi Id chiing d cung thdi diem Huyet sac td trong hdng cau cd nhiem vu tiep nhin oxy khi qua phdi va nhudng lai lugng oxy dd cho cac te bao khi qua cac mao mach. Dinh lugng huyet sac td cho biet rd chirc nang ciia hdng ciu.Hematocrit la ty le

% giua khdi hdng ciu va miu toan phan. Neu thudc lam thay ddi lam sd lugng hdng cau hoac lam mat nudc hay li nudc trong te bao mau thi chi s6 nay se thay ddi [2].

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Nghien cdu YDHCT Viet Nam Sd43 - 2014 Sau 8 tuan udng che pham vien

nang Dielsin, tat ca cac xet nghiem danh gia chiic nang tao mau d Id tri 1 va Id tri 2 khdng cd nhieu su khac biet cd y nghia so vdi Id chiing va so sinh giira cac thdi diem tnroc va sau khi udng thudc thu (p>0,05). Nhu vay vien nang Dielsin khdng the hien ddc tinh tren co quan tao mau.

V. KET LUAN:

5.1. Dpc tinh cap: Vien nang Dielsin khdng cd bieu hien ddc tinh

cap d lidu 53,33g cdm dugc lieu/kg.

Chua xac dinh dugc LD50 tren chupt nhat trang bang dudng udng theo phuong phip Litchfield - Wilcoxon.

5.2. Anh hirdng cua vien nang Dielsin len tinh trang chung va cac chi so huyet hpc:

Vien nang Dielsinlieu 0,18 g/kg/ngay theo dudng udng va lieu cao gip 3 lan (0,54g/kg/ngay) trong 8 tuin lien tuc, khdng anh hudng tdi cac chi sd huyet hpc tren thd.

TAI LIEU THAM KHAO

1. Nguyen Quoc Huy (2010), Nghien cuu ve thirc vat. thdnh phdn hoa hoc, mgt so tdc dung sinh hoc ctka mgt sd loai thugc chi Stephania Lour, 0 Viet Nam, Luan an Tidn sT Dugc hpc.

2. Nguyen The Khanh, Ph^m Tu Dmrag (2001), Xet nghiem sic dung trong ldm sang, Nha xuat ban Y hpc.

3. Camille Desgrouas.NicoIas Taudon (2014),Ethnobotany,

phytochemistry and pharmacology

of Stephania rotunda Lour, Journal of Ethnopharmacology, 154, p537-563.

4. Gerhard Vogel H. (2007), Drug discovery and evaluation Pharmacological assays. Springer.

5. World Health Organization (2000), Working group on the safety and efficacy of herbal medicine.

Report of regional office for the western pacific of the World Health Organization.