Nghien ciiru YDHCT Viet Nam
ilVGHlfeN CtJti TAG DUNG CUA VIEN NANG "GIANG CHI TIEU KHAT LINH"
TREN CAC CHI SO GLUCOSE VA TRANG THAI CHONG OXY HOA TRONG MAU Of CHUOT CONG TRANG DAI THAO DU'ONG TUYP 2 THlTC NGHIEM S t u d y on t h e effects of G C T K L ( G i a n g chi tieu k h a t linh) capsules on some
i n d i c a t o r s of glucose a n d a n t i o x i d a n t system in blood in e x p e r i m e n t a l diabetic w h i t e r a t t y p e 2
* Vii Viet HSng ', * Tran Thj Hong Phinmg ^ ' Khoa YHCT - Trudng Dgi hpc Y Hd Noi, ^ Bo Y ti Tom tat
Nghien cim dm?c thuc hien nhdm ddnh gid tdc dying ciia vien nang "Gidng chi tieu khat linh "
(GCTKL) tren cdc chi sd glucose vd trgng thdi chdng oxy hod trong mdu d chuot cdng trdng gay ddi thdo duang typ 2 thuc nghidm. Kit qud nghien cuu da chung minh GCTKL co tdc dung- (1) o chudt sau khi hi rdi logn lipid mdu gdy ddi thdo dudng typ 2, sau 60 ngdy diiu tri GCTKL liiu 840mg vd 1260mg/kg/ngdy dudng udng co tdc dung gidm cdc chi sd glucose mdu con 79,8% & 67,8% (2) Tdc dung tren trgng thai chdng oxy hod mdu: Hogt do GPx mdu a chugt ddi thdo dudng dugc diiu tri a lieu 840mg va 1260mg/kg/ngdy gidm it hem so vai cdc chudt khdng dugc diiu tri, s\t khdc biet vdi p
<0,05 va p<O.OL
Tir kho^: Gidngchi tigu khat Unh, GCTKL, dai th^o direrng, chong oxy ho4.
Summary
STUDY ON T H E EFFECTS O F GCTKL (GIANG CHI TIEU KHAT LINH) CAPSULES ON S O M E INDICATORS OF GLUCOSE AND ANTIOXIDANT SYSTEM IN BLOOD IN EXPERIMENTAL DIABETIC WHITE RAT TYPE 2 Objective of the study was to evaluate Peatment effects of GCTKL capsules on some indicators of glucose and antioxidant system in ~blood in experimental diabetic white rat type 2. The results showed that the GCTKL at doses of 840 mg/kg. 1260 mg/kg via oral after 60 days reduced concenPationof glucose at 79,8% & 67,8%: GCTKL at dose of 1260mg/kg/dqy is more effective than dose of 840 mg/kg/day (p<0,05). Effects on anti-oxidation: blood GPx activity in dyslipidemia and diabetic rats treated at dose of840mg and 1260 mg/k^day reduced less than untreated rats, the difference was statistic significant with p<0.05 andp<0.01.
Key words: Giang chi tieu khat linh, GCTKL, diabetic, anti-oxidation.
Dja chi lien h|: Vu Vi?t Hgng - Trutmg Dai h^c Y Ha Ngi Ngiy nhSn bai: 08/10/2014 Dja chi email: [email protected] Ngay phSn bi^n: 18/10/2014 Phan bifn khoa h^c: PGS.TS. Nguyin Nhirgrc Kim NgJy d3ng bio: 19/12/2014
*TS.BS
79
Journal of research in viet nam traditional medicine and pharmacy No 43' 2014 I. DAT VAN DE
Su thieu tuong dfii hay tuy6t doi insulin trong benh dai thao duong (DTD) la nguyen nhan gay thay doi cac con duong chuyen hoa ma truoe tien la roi loan chuyen hoa glucid.
Benh nhan DTD thudng duoc cho la chiu tac dong cua cac stress oxy hoa.
Cac nghien cuu dich te hpc da cho thay rang su tang glucose mau man tinh la yeu to nguy co cho su phat trien cac bien chung cua DTD. Khi nong do glucose trong te bao cao co the gay doc bang cac co che nhu tang chuyen hoa glucose theo con ducmg polyol, glucosamin, tao thanh. cac san pham tan (advanced glycation end- products AGE), hien tugng glycosyl boa khong enzym, tao thanh va boat hoa protem kinase C trong hang chuoi cac stress oxy hoa. S\r tao thanh AGE la khong thuan nghich, xay ra trong giai doan hang thang den hang nam va co the gay t6n thuong mo. Su tich luy AGE trong collagen co the lam giam tinh dan hoi cua mo lien ket (ton hai chuc nang mach mau) va lam ton thuong mang day ciia than. CJ nhung b?nh nhan DTD, AGE con co mat trong ca LDL ciia mau va cac ton thuong VXDM [1][2]. Theo cac thdng ke, bien chiing tim mach la nguyen
nhan hang diu gay tii vong 6 b?nh nhan DTD, vgly nen dieu tri RLLPM, kiSm soat duong mau, cai thiSn trang thai chfing oxy hoa mau la vin de thiet thuc trong dieu tri DTD [2][3]. Vi$c sii dung cac che pham YHCT co ngufin g6c thuc vat, CO tac dyng ch6ng oxy hoa dang la xu huong trong thoi dai hiSn nay. Giang chi tieu khat linh (GCTKL) tu bai thuoc nghiem phuang
"Giang chi thang" gia vj. Huong den ting dung tren ldm sang, chiing toi tien hanh tren thuc nghiem nham cac muc tieu:
1. Bdnh gid tdc dung ha glucose mdu cua vien nang Gidng chi tiiu khdt linh & chugt cdng trang gay BTB typ 2 thuc nghidm.
2. Bdnh gid tdc dung cua vien nang Gidng chi tieu khdt linh trin su thay doi trang thdi chSng oxy hoa trong mdu & chupt BTB typ 2 th^
nghiem
II. GHAT LIEU, DOI TU'QfNG VA PHirONG PHAP NGHIEN ClTU [4]
2.1. Chat lifu nghien curu: Vien nang "Giang chi tieu khat linh"
500mg.
- Noi san xuat: Cong ty CP Dugc phSm Khang Minh - TP. HCM
- Bai thufic: Dan sam, Sinh hoang ky, Ha thu 6 do, Trach ta, Nguu tat.
Nghien ciru YDHCT Viet Nam So 43 - 2014 ich tri nhan. Son tra, Hoang tinh, Linh
chi, Thien hoa phan, Hoang lien (tong 72g).
- Lieu thuoc du ki6n dung tren ngucri (50kg): 18 vien nang 500mg/ngay tuong duong 01 thang thuoc 72g, nghien ciiu tham do them lieu 2 bang 2/3 lieu tren.
- Lieu dimg tren chupt (he so 1:7):
Liau 1260mg va liSu 840mg /IkgTT/
ngay.
2.2. Doi tuQTig nghidn cmi: Chupt eong trang, chung Ratus norvegicus 2 thang tuoi, ca hai gioi, khoe manh, can nang 120 ± 20 g, nuoi trong dieu kien tieu chuan thuc nghiem tai khu chan nuoi dong vat thi nghiem benh vien Viet Nam Thuy Dien, Uong bi, Quang Ninh.
2.3. Phuong phap nghien cihi + Mo hinh gdy roi loan lipid mau:
Cho chupt an che do an giau lipid 40%
calo la ch4t beo (theo bang thanh phan dinh duong thuc pham Viet Nam, Vien Dinh duong, Bp Y ta, Nha xu4t ban Y hpc 2000) trong suot qua trinh thuc nghiem.
+ Mo hinh gay dai thao duong thuc nghiem: Ngay thii 31 cua roi loan lipid mau, chupt dupe tiam mang bung STZ voi nau 50mg/kg (mot liau duy nhat). Sau 48 dan 72 h, dinh lupng
nong dp Glucose mau dat > 11 mmol/l la dat yau cau chupt dai thao duong.
Chupt dupe chia ngau nhien thanh 516
- Lo chirng: n = 21, chupt dupe nuoi vdi cha dp binh thuong 12%
calo khau phan an la lipid (theo bang thanh phan dinh duong thuc pham Viet Nam, Vien Dinh duong, Bp Y ta, Nha xu4t ban Y hpc 2000) va uong dung dich NaCl 9%o lieu 1 ml/1 OOgam/ngay.
- Cac 16 1, 2, 3, 4 : Chupt duoc gay roi loan lipid mau; trong do cac 16 2, 3, 4: Sau khi gay roi loan lipid mau, ngay thii 31 chupt dupe gay DTD bang phuang phap tiem mang bung STZ voi lieu 50mg/kg (mpt liau duy nhat). Sau 48 dan 72 h, dinh lupng n6ng dp Glucose mau dat > 11 mmol/1 la dat yau cau chupt dai thao duong.Trong suot qua trinh thuc nghiem, cung v6i che dp an giau lipid 40% calo la chat beo, chupt cac 16 nay dupe uong them:
+ L6 1 (chiing duong gay RLLPM) .. = 21, u6ng NaCl 9%o liau 1 ml/1 OOgam/ngay.
+ Lo 2: n =14, Sau khi gay DTD, u6ng NaCl 9%o liau Iml/lOOgam/ngay.
+ Lo 3: n =14, Sau khi gay
Journal of research in viet nam traditional medicine and pharmacy No 43 - 2014 DTD, uong GCTKL lieu
840mg/kgTT/ngay.
-^ Lo 4: n ^14, Sau khi gay DTD, u6ng GCTKL liSu 1260mg/kgTT/ngay.
- Ngay thii 30, 60 va 90 voi 16 chiing va 16 I giet 7 chugt/ 116
- Ngay thii 30, thii 60 sau dilu tri, cac 16 2, 3 va 4 giSt nglu nhien 7 chupt/116.
Tai cac thai diem giet chuot dinh lupng glucose mau, TC, TG, LDL-C; HDL-C, TAS huySt tuong, hogt dp SOD hong cau, GPx mau toan phjn, THb (Total Hb).
Dinh lupng Glucose mau (tinh theo don vj mmol/1), hoat do SOD
h6ng cSu (tinh theo don vi U/ml), GPx mau toan phSn (tinh theo dcm vj U/1) va THb (Total Hb tinh theo don V! g/dl).
Cac xet nghiem sinh hoa dupe th\rc hien tren may phan tich sinh hoa tu dpng OLUMPUS - AU 400 (Nhat) vdri cac kit h6a chat hang Olympus cung cap tai Khoa Hoa sinh, benh vien Viet Nam - Th\iy Dien, Uong Bi - Quang Ninh.
Sau khi dinh lupng SOD, GPx, Hb tren may, tinh ket qua SOD va GPx theo don vi U/gHb theo cong thiic sau:
SOD(U/gHb) =
GPxU/gHb =
SOD (units/ml) Hemoglobin (g/ml)
U/lOOml Hemoglobin (g/l 00ml)
Ket qua nghien ciiu dupe xu ly bang thuat toan thong ke Y hpc, bieu dien duoi dang gia tri trung binh, do lenh chuan va gia tri trung vi (TV). C& mau nghien ciiu la CO mau nh6, s6 lieu thu dupe phan b6 kh6ng theo quy luat chuan. De so sanh su khac biet giOa 2 nhom su dimg thuat toan wilcoxon rank-sum test (Mann-Whitaey).
Nghien citu YDHCT Viet Nam III. KET QUA NGHIEN CUtJ
3.1. Anh hu-ong chi dp an gi&u chat beo tren cac chi so lipid mau if chuot cong trang
Bang 3.1. Anh hudng cua che do an giau chat beo tren cac chi so lipid mau cua chupt cong trang
ThM dilm
Ngay 31 (D31)
Ngay 61 (D61)
Ngay 91 (D91)
Lo n = 7
Chung
L6 1 P Chiing
L6 1 P Chiing
L6 1 P
Cac chi so lipid (mmol/L) TG
X±SD 0,81 ± 0,26 1,49 ±
0,20
<0,01 0,86 ± 0,15 2,37 ±
0,29
<0,01 0,94 ± 0,25 2,73 ±
0,37
< 0 , 0 I TV 0,7
1,5
0,8
2,5
0,9
2,7 TC X±SD 1,41 ±
0,19 1,96 ±
0,3
<0,01 1,00 ±
0,05 2,39 ±
0,26
<0,01 1,31 ±
0,20 3,57 ±
0,56
< 0,001 TV
1,4
1,9
1,4
2,4
1,4 3,5
HDL-C X±SD 0,82 ± 0,13 0,74 ±
0,26
>0,05 0,88 ± 0,09 0,71 ±
0,15
<0,05 0,81 ± 0,15 0,70 ±
0,14
>0,05 TV 0,85
0,72
0,88
0,65
0,83
0,65 LDL-C X±SD 0,36 ± 0,11 0,58 ±
0,26
>0,05 0,33 ± 0,06 0,91 ±
0,27
<0,01 0,26 ± 0,07 1,66 ±
0,62
<0,01 TV 0,33
0,53
0,33
0,90
0,27
1,85 HDL/
LDL
X 2,44
1,42
<0,05 2,77
0,87
<0,01 3,10
0,50
<0,01 L6 chiing: an chS dp an 12% la lipid, 16 1: an ch6 dp an 40% la lipid
Nhan xet: Cac chi so TG, TC, LDL-C dSu co su thay dii co y nghla (p< 0,05) 6 chupt 16 1 gay RLLPM so vcri 16 chung va 6 ca 3 thoi diem sau 30, 60 va 90 ngay.
Journal of research in viel nam traditional medicine and pharmacy No 43 - 2014 3.2 Anh huwng che df an giau chat beo tren ho^t do SOD hong cau, GPx mau toan phan va TAS huyet tirong il chuot c6ng trjng
Bang 3.2. Anh hvong cua che do an giau ch^t beo tren nong do TAS huyet tirotig, hoat do SOD, GPx o chu$t cong trang co che dp an giau lipid
Thfri diem
Ngay 31 (D31)
Ngay 61 (D61)
Ngay 91 (D91)
Lo (n=7)
Chimg
Lo 1 P Chimg
Lo 1 P Chiing
L6 1 P
TAS (mmol/1) X±SD 1,43 ± 0,08 1,49 ±
0,13 TV 1,47
1,48
SOD (U/gHb)
X±SD 2239 ± 1 7 7
2290 ± 497 TV 2177
2352
GPx (U/gHb)
X±SD 752 ± 7 4
774 ± 1 5 3
>0,05 1,54 ±
0,11 1,37 ±
0,09
<0,05 1,59±
0,30 1,38 ±
0,10 1,51
1,35
1,51
1,36
2298 ± 1 8 1
2 1 0 5 ± 1 5 1
<0,05 2239 ± 1 3 5
2126 ± 1 6 4
>0,05
2241
2110
2309
2133 745 ± 68
620 ± 4 1
<0,05 765 ± 50
621
<0,05 TV 745
799
755
639
759
621 THb (g/L) X±SD 155±13
157±31
154±11
153±15
158 ± 9
147 ± 5
L6 chung: an che dp an 12% la lipid, 16 1: an chS dp an 40% la lipid
Nhan xet: Sau 60 ngay, cac chi s6 TAS huyjt tuong, hoat dp SOD va GPx giam hon so voi 16 chiing (p<0,05). Sau 90 ngay cac chi s6 TAS va SOD 16 1 giam kh6ng CO y nghia p>0,05, boat dp GPx giam co y nghia p<0,05.
Nghien cUu YDHCT Viet Nam
3 3 . Tac dung cua GCTKL tren chi so glucose mau chuot cong t r ^ g DTD typ 2 Bang 3.3. Tac d^ng ciia GCTKL tren nong do glucose mau chuot D T D typ 2 Lo
n = 7 L o ! L6 2 L6 3 L6 4 P(2,3) P(2,4)
Ndng do glucose (mmol/L) Sau 30 n g i y dieu tri (D61)
X±SD 5,63 ± 1,15 16,35 ± 1,43 13,20 ± 2 , 1 6 12,51 ± 1,76
<0,01
<0,01
TV 5,6 16,5 13,5 12,8
Sau 60 ngay dieu tri (D91) X±SD
5,57 ± 0,96 17,70 ±1,64 14,13 ± 0 . 9 0 12,01 ±2,45
<0,01
<0,01
TV 5,4 17,4 14,7 12,8
% so vM Id 2
79,8% (4.) 67,8% (4.) p(3,4) < 0,05 L6 1 va 2 uong NaCl 0,9%, 16 3 uong GCTKL lieu 840mg; 16 4 uong GCTKL liau 1260mg,
Nhan xet : o ca hai thai diem sau 30 va 60 ngay dieu tri, 6 16 3 va 4 nong dp glucose deu thap hon co y nghia vai p<0,01 so voi 16 2 va giam con 79,8% va 67,8%
6 thoi diem sau 60 ngay diau tri. Chi so glucose man 6 16 4 giam t6t hon 16 3 co y nghla th6ng ke vai p<0,05 sau 60 ngay diau tri.
3.4. Tac dung cua GCTKL tren hoat dp SOD h^ng cau, GPx mau va TAS huyet tuong if chupt cong trang dai thao duong typ 2
Bang 3.4. Tac dung dieu tri cua GCTKL tren nong do TAS huyet tuong va ho?it d6 SOD, GPx if chupt cong trang dai thao dirorng typ 2
ThM diem Sau 30
ngay di€u tri (061)
Sau 60 ngay dieu tri (091)
Nh6m (n=7) Chiing L6 2 L6 3 L6 4 P(2,3) P(2,4) Chiing L6 2 L o 3 L6 4 P(2,3) P(2,4)
TAS (mmol/l) X±SD
1,54±0,11 1,38 ± 0 , 1 5 1,41 ± 0 , 1 9 1,43 ± 0 , 1 2
>0,05
>0,05 1,59 ± 0 , 3 0 1,36±0,13 1,42 ±0,17 1,46 ± 0 , 1 0
>0,05
>0,05 TV 1,51 1,32 1,30 1,45
1,51 1,39 1,32 1,47
SOD (U/gHb X±SD 2298 ± 181 1998 ± 266 2251 ± 2 5 7 2216± 110
>0,05
>0,05 2239 ± 1 3 5 2066 ± 185 2221 ± 142 2234 ± 1 6 5
>0,05
>0,05 TV 2241 2053 2186 2186
2309 2249 2006 2192
GPx (U/gHb) X±SD 745 ± 68 603 ± 49 730 ± 63 723 ± 67
<0,01
<0,01 765 ± 50 613 ± 5 6 715 ± 4 5 709 ± 47
<0,01
<0,01 TV 755 600 710 740
759 618 710 712
THb (g/L) X±SD 154± 11 155 ± 2 0 156± 14 165 ± 16
158 ± 9 159± 17 163 ±14 166 ± 15
Journal of research In viet nam traditional medicine and pharmacy No 43-2014 L6 2 u6ng NaCl 0,9%, 16 3 uong
GCTKL li6u 840mg; 16 4 u6ng GCTKL lieu 1260mg
Nhan xet: Tren chi s6 TAS huyet tuong va boat dp SOD h6ng cau: So sanh voi 16 chiing 6 thoi diem D61 va D91, 6 16 3 16 4 CO chi so thap hon nhimg su khac biet kh6ng co y nghia voi p>0,05. d 16 2 (DTD kh6ng dupe dieu tri) n6ng dp TAS va boat dp SOD giam ban so voi 16 chiing voi p<0,05.
So sanh voi 16 3 va 16 4 thi chi s6 TAS va SOD ciia 16 2 co giam hon nhung kh6ng CO y nghia th6ng ke voi p>0,05.
Tren boat dp GPx mau toan phan:
Hoat dp GPx 6 16 3 va 16 4 giam hon so vcri 16 chiing, sir khac biet khong c6 y nghia thong ke voi p>0,05 6 thai i GPx man toan phan
diSm D61 va D91. N6ng dp GPx cila 16 2 deu giam hon so voi cac 16: 16 chiing, 16 3 va 16 4 CO y nghia voi p<0,01 a cac thoi diSm D61 va D91.
Nhu vay or ca hai thai diSm D61 va D91, 616 2 (DTD kh6ng dupe dieu tri) boat dp GPx giam hon so voi cac 16 dupe diSu tri Giang chi tieu khat linh (co y nghia voi p<0,01). Cac 16 3 va 4 chupt DTD nhung dupe dieu tri bjng GCTKL a ca hai lieu thi boat dp GPx CO giam han so voi nhom chiing nhung su khac biet khong co y nghia th6ng ke voi p>0,05.
Sir thay d6i boat dp GPx mau toan phan cua cac 16 DTD dupe di6u tri bing GCTKL va khong dupe diSu trj dupe thj hien ro ban a hidu d6 sau:
•• :iK0,01
• L6 churns
• LdZiOTD
• t a 3 : OTtMiCTKL SWing
• Ls 4: DTtHGCIKL 1260m|
SIU 30 ncjiv tflSu trj (DSI)
S.U SO ngiy dAu tit ID91)
Th6i di^m
Bieu do 3.1: Hoat do GPx mau toan phan it chupt cong trSng gay dai thao diromg typ 2 dieu trj GCTKL lieu 840mg va 1260mg
Nghien ctJcu YDHCT Viet Nam IV. BAN LUAN
Thuc nghipm gay r6i loan lipid mau a chupt voi ch6 dp an 40% calo khau phan an la ch§t beo trong nghien cuu da dat yeu cau. Ngay 6 ngay thii 30 hau het cac chi s6 ve lipid mau 6 nhom chupt an theo cha dp giau ch4t beo da tang nhiau (tru HDL- C). Dac biet chi so LDL- C tang cao va ty so HDL- C/LDL- C giam, sir khac biet co y nghia th6ng ka so voi 16 chung, cho thay da xuat hien yeu to nguy co gay vija xo d0ng mach 6 chupt.
Mpt so nghien cuu cua cac tac gia trong va ngoai khac dau co nhSn xet chung la cha dp an giau chat beo gay tang ro r^t nong dp TG, TC va LDL-C trong mau chupt luc doi. Chupt duoc nuoi boi cha dp an giau lipid co su giam nhay cam voi insulin va chi can mpt lieu thap STZ ciing dii gay DTD.
Sau 90 ngay nghian cuu, n6ng dp TAS huyat tuang, hoat dp SOD va GPx h6ng cau giam so vai 16 chiing thi chi CO sir khac biet d boat dp GPx giam hpn 16 chiing voi p<0,05.
Nhu v|iy mo hinh gay DTD typ 2 da thanh c6ng, dap iing yeu cau cho nghien cuu
4.1 Tac dung diau tri tren chi so glucose mau ciia Giang chi tieu khat linh sau 30 ngay va 60 ngay uong thuoc
Bang 3.1 cho thay nghien cuu da thanh c6ng trong viec gay DTD typ 2 cho nhiing 16 chupt bi RLLPM bSng each tiam mang bung mot liau thap STZ 50mg/kg. Trong nghien ciiu nay cho ket qua sau 30 ngay va 60 ngay u6ng thuoc, n6ng dp glucose huyet thanh ciia chupt 16 3 va 16 4 (hai 16 chupt DTD duac u6ng GCTKL) giam CO y nghia th6ng ke so vai !6 2 (chupt DTD kh6ng dupe u6ng GCTKL) (p<0.01); Nong dp glucose mau thay d6i chiau huong t6t hon a liau GCTKL 1260mg (p<0,05). Ket qua nay tuong duong voi ket qua cua Nguyan Quang Trung nghien ciiu 2008, bpt chiat la dau CO tac dung giam n6ng dp glucose mau cua chupt gay DTD typ 2 sau 30 ngay va 60 ngay la 71% va 77%.
GCTKL CO tac dung giam n6ng dp glucose mau 6 chupt gay RLLPM va DID thuc nghiem, nhung kh6ng lam giam glucose mau o chupt khong bi DTD. Dieu nay rat co y nghia trong y hpc va day cung la mpt uu tha ciia thuoc y hpc co truyen, thuoc co chi co tac dung voi cac truong hop benh ly va khong anh huong toi cac trucmg hpp binh thucmg. Tian tai nghien ciiu tran lam sang, Giang chi tieu khat linh co the dung ha glucose mau cho benh nhan co duong mau cao.
Journal of research in viet nam traditional medicine and pharmacy No 43 - 2014 4.2 v a tac dung dieu tri cua Giang
chi tieu khat linh tran trang thai chong oxy hoa trong mau or chupt cong trang gay dai thao duong typ 2
Trong nghien ciiu nay, sau 30 va 60 ngay diau tri: Nong do TAS huyet tuong, hoat dp SOD cua cac 16 chupt dai thao duong dupe diau tri va kh6ng dupe diau tri dau giam so voi 16 chiing, sir khac biet nay kh6ng co y nghla thong ka voi p>0,05. So sanh voi 16 DTD kh6ng dupe dieu tri, thi hai 16 DTB duoc diau tri bang GCTKL giam it hon, tuy nhien su khac biet nay kh6ng CO y nghia th6ng ke vai p>0,05 (bang 3.4). Tren hoat dp GPx: Lo chupt DTD kh6ng dupe dieu tri giam so vai 16 chiing va hai 16 DTD dupe uong GCTKL, su khac biet co y nghia th6ng ke voi p<0,01) (Biiu d6 3.1).
4.3. Ly giai ve tdc dting cua Gidng chi tieu khdt linh
Theo chung t6i, so dT co ket qua nhu tren la do trong thanh phan bai thu6c GCTKL gom cac vi dau bang thuong dupe dimg de 6\hx tri chiing tiau khat nhu Linh chi, Thien hoa phan (Radix Trichosanthis), Hoang lian (Rhizoma Coptids), Hoang tinh, Hoang ky. Theo ly luan ciia y hpc co truyan, Linh chi tac dung tiau dam, lgi nieu, ich vi; Hoang ky tac dung on b6 ty
duang, sinh tan, chi khat; Hoang lien tac dung ta hoa, tao thip; Hoang tinh tac dung b6 pha sinh tan, tri ty vi hu nhupc, pha khi hu; thien hoa phin tac dung sinh tan, chi khat giang hoa, nhuan tao, bai thiing, ti6u thung; vi vay bai thu6c co tac dung tri chiing tiSu khat [6].
Bang phuong phap sac ky long hieu nang cao da xac dmh duoc cac boat chat co trong Giang chi tieu khat linh: acid oleanolic vdi ham lugng l,021mg/lg c6m tanshinone HA voi ham lupng 0,032 mg/lg. Acid oleanolic duoc chiing minh do la mpt chat lie cha manh me ciia qua trinh viam ta bao, tac dung lam giam su hinh thanh cholesterol va tang bai tiet cholesterol.
Tanshinone IIA c6 tha thiic day hoat dpng ch6ng viem, ch6ng oxy hoa, va gay dpc ta bao, va ciing nhu tao ra qua trinh apoptosis. Tanshinone KA ciing da dupe chiing minh la giam viia xo dpng mach do lic che yau to tang truong tieu cau, lie che sir di chuyan cua cac ta bao co tron a lop trung mac ra lop npi mac cua thanh dpng mach chu la cac nguyen nhan ehinh gay vGa xo dpng mach [7][8]. Mpt s6 cac hoat chit khae nhu emodin, astragalosid IV cung dugc xac nhan co mat trong thanh phin ciia thuoc. Tac dung cua
Nghien cuu YDHCT Viet Nam So 43 - 2014
astragalosid IV (chat nay co nhieu trong vi hoang ky va Dan sam), lam giam miic dp malonyl dialdehyd, mot chat chuyen hoa ciia qua trinh peroxy lipid va lam tang miic dp chat ch6ng oxy hoa enzym glutathion peroxidase va superoxid dismutase trong cac m6 [9]. Tuy nhien can co nhung nghien ciiu sau bon trong tuong lai ve co che va tac dung ciia Giang chi tieu khat linh trong diau tri r6i loan lipid mau va
ch6ng viia xo dpng mach [9].
V. KET LUAN
Giang chi tieu khat linh lieu 840mg va 1260mg/kg/ngay duong u6ng sau 60 ngay diau tri co tac dung:
1. Lam giam nong dp glucoza mau tran chupt DTB typ 2 (p<0,01)
2. Lam boat dp GPx tren chupt DTD typ 2 giam it ban so vai 16 kh6ng dugc dieu tri (p<0,01).
TAI LIEU THAM KHAO L Nguyen Trung Chinh, Tr^n Dinh
Toan (2000), Tdng Cholesterol mau benh thai dgi, Nha xuat b ^ Y hpc.
2. Ph^m Tii Duong (2002), "RSi loan lipid mau va benh vua xo dong mach", Bdi gidng sau dgi hpc C\ic qudn Y, Ha Npi,tr. 11-18.
3. Alan R.L. (1994), "Cholesterol and triglycerid". Free Radical, Biology and Medicine.
4. Kedziora KZ, Luciak M, Blaszczyk J, Pawlak w (1998), Lipid peroxidation and activities of antioxidant enzymes in erythrocytes of patienls with non-insulin dependent diabetes with or without diabetic nephropathy, Nephrol- Dial-Transplant. 13(11), pp. 2829- 2832.
5. Pham Khue (2000), "VGa xo dpng mach", B4nh hgc tuoi gia. Nha xuat ban Y hpc. Ha Npi, tr. 178-202.
6. Hoing Bao Chau (1997), "Tieu khat", Ngi khoa y hgc co truyen, Nha xuat b^n y hpc Ha Npi, Tr. 377-384.
7. Astragalus membranaceus (Monograph), Alternative Medicine Review, Volume 8, Number 1 - 2003, pp. 72-77.
8. Jin UH, Suh SJ, Chang HW et al (2008), Tanshinone IIA from Salvia miltiorrhiza BUNGE inhibits human aortic smooth muscle cell migration and MMP-9 activity through AKT signaling pathway, / Cell Biochem. 2008 May 104(1), pp. 15-26.
9. Bao-Qing Wang (2010), Salvia miltiorrhiza: Chemical and pharmacologicalreview of a medicinal plant, Joumal of Medicinal Plants Research, Vol. 4(25), pp. 2813-2820.
