HOI NGHI KHOA H p C C O N G NGHS SINH HpC T P A N Q U 6 C 2013
NGHIEN CLfU XAY DI/NG QUY TRINH SAN XUAT PHAN VI SINH VAT CHO CAY LAC TRCING TREN DAT CAT BlfiN
^ NguySn Thu H i ' ' v i Phgm Van Toin*
'Vl^n ThS nhifdng Ndng hda, Vl^n Khoa hgc Ndng nghiep Vlkt Nam
^Vi$n Khoa hgc Ndng nghl$p W0t Nam
T6M TAT
EH li;a Argc chgn td hgp vi sinh v^t cho cay lac t r ^ g tren d&t c&t b i ^ gdm chung co tdii nang phln giai silicat {Paenibadllus castaneae SJ.l), pbSn gi^ pbh\ phit kh6 tan {Badllus megaterium P1107), c6 djnh mto c5ng sinh cay I51C {Bradyrhizobium Japonicum RA18) vk sinh potysacarit {Lipomyces starkeyi FT S.l). D3 n ^ e n cuu xfiy dgng qm trinh san xuit phan vi slnh vJt chuybi dgng cbo cay l?c IrMg trSn dit cat b i ^ Phan vi sinh vJt dat chit lirgng theo thdng tu 36'2010/TT-BNNPTNT (mgt d!) le bio vi linh vjlt mfii loai dft 10* CFU/g, thdi gian bao quin 6 thang).
Tir khda: Cky l?c, dit cit bien, phin vi sinh v$t, vi khuin ch dinh nito, vi khuin phan giii phot phit kho tan, vi khuan phan giii
• silicat, vi khufin sinh polysacarit
Uc {Aradils hypogaea L.) ik m$t cSy cflng nghi$p ngan ngdy, c6 khk nang Biich i>ng rOng vk eky lly dhu co gi^ trj kinh te cao Lgc cdn \k m$t hSng nflng san xuat khiu quan Irgng (chiSm 50 - 60% tong skn lu-png) vk la c^y cdi tao tang dfl phi nhigu dSI ly ti/^ng (Ngd Th6 Dkn. 2000).
Bit cdt blAn duvc xfip vko nh6m dll cd dd phi nhifeu thip, thSnh phSn co- gidi nhg (chu ylu 1^ c^t), r l l nghfeo dinh dif ng, khS nang glO nude vk phkn bfln kem, d5 b6d hoi vk n>a trfli m^nh. Dat c^t bifin b Vl#t Nam c6 dlgn tlch khodng 530 nghln ha. Ph9n bo chu ylu d Bac Tmng Bfl va duySn hk\ miln Tmng. Trong ckc tinh thuOc B^c Tmng Bd vk duySn hdl miln Trung, c^y l^c cfl dign tich Irong khk lem. Theo Tfing cgc Thing ke, di$n llch IrAng l^c t^i vCing B5C Tmng Bo va duyfin Ml miln Tmng nSm 2011 la 101,1 nghln ha; trong dfl di$n tlch trong lac IrSn dit c^l biln chilm 70-75% fNifln giam thflng kfi, 2011),
Vai trfl khOng Ihl thilu cua phkn vi sinh v | l Irong vi#c nSng cao dinh dirong, Vhk nkng gid nirflc, giO Im dat; qua dfl l^m tang kha nfing sinh tnjbng phkt triln, tSng nang suit cay trfing cOng nhi/ tilt kigm phSn bfln hoa hgc, giam thilu thuic bao v$ thvc vkt hoa hgc va gflp phln tlch cyc cho vigc xay dyng nfln nong nghiep bin vQ-ng d i dif^c khlng djnh Irong nhlfiu cflng trinh nghifin oXi (Phgm van Toan, 2005; Phgm Van Toan, 2008; Tong Klm Thuin, 2005). Tuy nhifin hi$n nay chira c6 phSn vi sinh v^t chuy&i dgng cho cSy Igc diwyc trfing tgi viing dfit cat biln, Myc tifiu cOa d l tai la xay dyng . .qui trinh san xuat phan vi sinh v$l chuyfin diing cho cay Igc trfing Igi vilng dll cat biln.
NGUY£N L i f u VA PHlrtTNG PHAP Nguyfin ll^u-
S!f ' * ' ^ ? J ^ !'"^ " ^ ' - ' ^ ^ •*""? ^ slnh v9l cfl kha nang c6 djnh nito RAie, phan gidi phfit phat khfl tan P1107 phSn g^l sihcat S3.1V& sinh chit glO Im polysacarit PT5 1. Cke chung vl sinh v|t di/gc tuyfin chgn ti> quy gen vi sinh 4 vk skn pham cOa dfi tSi: Nghifin CLPU pha triln ede giai phap slnh hgc nham ck\ tgo dat bgc mSu".
P/e^*^L.»^i' ®,?^fV."^ *^° ^^"^ ^° '"*' *^^"9 ""^' ^'^ ^ s'"^' v$t: NaCI. CaCli, CaCOa, Ca3(P04)2, MgS04 7H2O MnSG. KNO,, KCI, K,HP04, KH.PO,. FeS04, (NH.jzSO^. FeCfe, glucoza, pepton, cao nim m4n, bat nim men ri d^Sng!
fi?!!n"Sh^^t*^* ' " n ' i T ^ ^ J ™ " L f ^^ " ' ^ ' - ^ ^ " ^'""^ ^^*- "r*^^" ^^"= ^*^-^ ^ 12%. kfchIhydc hgt < 0,1 mm. pH =
<b 1 mm S ^ 6 , l 7 0 ' ' ^^' " ° ' ^ "''"• ^"^ = ^'^^•° "^ "^^^ ^90: Dg Im < 12%, kich thirdc hgt Phuwig phap
Xic dinh m$t dd vl slnh v^t: Theo TCVN 4864.2005 Binh gla hoft tinh slnh hgc cOa cic chung vi slnh v$i
• ™ « t i % ™ l ; i 8 ' M o ' f ' " " " ° " ^ " " " " ^ " " " '^° * ' " ' " " " * ' * '^""""-»! '•"""^ » » * ™ < = » " * * • 91="
moan 10 msohj.nrt ma VI smh vjl phan giSiCaslPO,), (TCVN 6167:1996) ' ' ' ' ' ^ " 1 '
^O^Wn"to™"» A h ! f ' " T i " . * " " ' " ? ^ " ^ " 9 P^^P * ' ' n g phan giai bOt IhOy tinh IrSn mdi Irirtng die; J6 la
^ C , S ' ^ \ 1 v« pTa^gia^ftM^^g.* *"""'""' '^ **"" " * " * ' * '"* ™ •"""' •""•'''™'' '»
H O I N G H I K H O A HQC CClNG NGHE SINH HOC T O A N Q U O C 2013
- Xac djnh kha ngng sinh polysacarit thflng oua dfl nhcrt cua dich nufli d y b i n q nhot k l nfit\walfi n $ nhii-t rJia riirh nnfti c l y du'g'c tinh ttieo cflng ttiu-c sau: n = (ni x d x t)/(diXT,)
Trong dfl n i : dfl nhfl'tcua nu-dc a 30° C, d i : ty trgng cua n u o c c r 30°C, T,. thdi glan chSy ciia dflng nu'fl'c, d: tl trgng cua dich nghifin ci>u vk t : thcrl gian chay cua dich nghifin ci>u.
Nghien ctru dleu kl^n nhin slnh khdi: Lya chgn dieu kl#n nhan sinh khfii thich hg'p thflng qua cac thi nghi$m vfi danh gia anil hu'fl'ng cOa mfli t r u i n g , thcrl gian, nhi§t dfi, pH v a i\iu i\iqng d p khi d i n mat dfi l l bSo vi sinh vgt.
Phuvng phip skn xuat phkn vi slnh v$t
- C h u i n b[ c h i t mang: Than bOn.tinh bflt s i n , cam ggo. Than bun dtf5rckhi> trting d 121° C, trong 60 phut. Tinh bgt s i n , cSm ggo d u ^ c khO tning d 115'C, trong 30 phCiL .
- Phfii trdn djch vi slnh v | t Tinh bflt s i n (60%). ckm gao (30%), djch vl sinh v | t (10%).
- BSo qukn: Skn p h i m du'g'c dflng gfli trong tdi thific, bao quSn d d i l u kien nhi$td5 phflng - Kiem tra: s a n p h i m d u v c kifim tra m^t dg vi sinh vgt theo thcrl gian bao qudn 1 , 2 , 3 , 4 , 5 va 6 thang.
K^T QUA vA THAO LUAN T u y e n c h p n t f i h g p ckc chOng v i s i n h v | t
Cac.chOng vi sinh v$t Si> dgng du'g'c t u y l n chgn tir quy gen vi sinh v$t trfing trgt ( d i t . phSn bfln) va san p h i m cda dfi tai:
"Nghien cdu phkt trifin c^c giai_phap-slnh hganham-cai-tgo.dlt bgc mau". Tfin va hogt tinh sinh hgc cua cac chung vi sinh v | t Slip dgng d u ^ c tfing hgp trong Bang 1.
Bang 1. TAn vi hoft tinh slnh hgc cCia cdc chiing vl slnh v^t t u y l n chgn
STT Chiing vl slnh v9t Hogt tinh sinh hgc Mile hogt Knh
1 Paenibadllus castaneae S3.1 Phan giii ^licat 12,0 (dufrng kinh vflng phdn gidl b^t thCiy tinh, mm)
2 LIpomyees steri&yl PT 5 1 Slnh c h i t giO I m polysacarit 37,6 x 10^
(d$nh*tT]IAs/m^
3 Badllus megatenum P1107 PhSn glii ph6l phit khfl tan (du*ng kinh vdmg 18,0 phin glil Cai(P04)2. mm)
4 Bradyrhizobiumjeponlcum RA18 C6 djnh ntto c9ng slnh c3y l^c 3 458 (him li/gng etylen hlnh thanh. nmol CjHJgid)
Khk nang tfin tgi va ho?t tfnh slnh hgc ciJa cac chCing vi slnh vgt trong tfi hg'p 6 d i l u kl#n rifing lfi va tfi h(?p sau b i o , quan la mOt trong d c chi tifiu danh gia kha nSng tfi hgrp o i a cac chiing vi sinh v | l K i t qua dip gc thfi hign trong Bang 2 .
Bang 2. K h i nioglAn tfil vk ho^t tinh slnh hgc cila c i c chiing vl slnh vJt trong ti h w STT
1
2
3
4 Ky hl$u ChOng VSV
S3.1
P T 5 1
P1107
RA18
C6rig thilc ^
Bon chCing T6hgp Den chung To hgp Don chiing Tfi hgp DonchOng Tfihgp
M$td$tet>io (CFU/g) T n r ^ bio quin Sau b i o quin ISO ngiy
4,8 X10' '4,4x10"
3,8 X 10"
3,5 X10"
4,8 X10"
4,6x10"
4,4x10"
4,2 X10'
2.4x10^
2,2x10"
2,0x10*
1,8x10"
2,8 X 10"
3,0x10"
2.8x10"
2,6x10"
Hoat Unh smh h?c chfnh sau 6 thing bio quin Dufrng kinh vdng phln glil sil'cat;12,0mm . - - - = - Duong kfnh vdng ph9n gjil
silicat; 12,0 mm D^nhM 37,6.10^ N.s/m' Da nhiSt 36,6 10^ N.s/m' BLfftng kfnh v6ng phin giii Ca3(P04)2,18,0 mm Du'dtig kfnlT "iing phin giii Ca3(PO<)2,18,0 mm Ham lirgng etylen hlnh thinh, 3.458 nmolCzH*/ciy Ham luvng etylen hinh thinh, 3 448 nmolCiH4/cay
K i t Qua Bang 2 cho t h i y : M$t dfi Is b i o va'hogt llnh sinh hgc cua cac chung vi slnh v | t I^ra chgn trong d i l u ki$n tfi hgrp v i rifing IS cfl s i / sai k h i c khflng dang k l sau 6 thang bao q u i n . Difiu nay chi>ng to eic chung vl slnh vit t u y l n chpn cfl - khi'nSng ciing phat t r i l n , khflng cgnh, tranh va i>c chfi nhau.
Dleu kign nhSn sinh khfii cOa ckc c h i i n g v i s l n h vgt t u y l n c h o n
M i l chung vl slnh vkt cfl d i l u kl$n nhan slnh khfii t l i thich khae nhau. Vi v | y , vfl'i mgc dfch thu dugc sinh khfii cdc chOng sinh vai cao n h l l , chiing tfll da t i l n hanh nghifin CO'U mflt sfi dieu ki$n thich hgp cho q u i trinh n h i n sinh khfii c ^ chOng
HOI NGH! KHOA HQC C O N G N G H E SINH HQC T O A N QUQC 2013
K i t qua danh gia anh ht/crng vi sinh v^t nhir: Mfll tn/drng, pH. nhiat dfi. , thoi gian Ihu sinh khfii, t J j f giong cap '• v - V - K^t qua danh g.a cua d c difiu ki§n nhan sinh k h l i den m^t do tfi bao vi sinh vgt d u g c t h i hign trong d c Hlnh 1, 2 , 3 . 4 , 5 va 6.
TCr k i t qua cac Hinh 1,2, 3 . 4 , 5 va 6 d l tai da l y a chgn d l l u kifin nhSn sinh khfii d y a trfin cac tifiu chf: M§t dfi t l bao dgt trfin 10* CFU/ml, flit ki^m thcrl gian nhSn sinh k h l i va gia thanh san p h i m . T i r cac kfil quk trfin chung tfli da l^a chgn ffiku kl$n nhfin sinh khfii cho ckc chiing vi sinh v^t t u y l n chgn n h u sau:
B i n g 3. Thfing sd k f t h u ^ cho q u i trinh n h i n sinh khfii c i c chung vi slnh v | t t u y l n chgn Tyl#
Tin Chflng Vl Slnh wit ^ ^ ^ n t ^ ^ PsenJtodDUs castaneae S3.1 5 U^om>TWSs(aritey/PT5.1 3 Bacillus megatenum P110?' 5 Bradyrhlzobium japonicum 5 RA18
M6i truong nuoi c i v silicat
SX3 SX2 YM
Nhi^t dfl Thdi gian Luu lugng i^p khfing khi L| [OQ^ l i n men (lit khflng khinitmfii 7.0±1 30±1 36 0,75 6,5±1 30±1 36 0,65 7,0±1 25t1 36 0,75 7Q^1 30±1 84 0,65
c a c chiing vl slnh vfit sau khi nhan sinh khfii dircrc d i n h gia Igi hogl tinh sinh hgc. K i t qua d u ^ c trinh bay trong Bang 4 . B i n g 4. Hoat Unh slnh hgc chlnh cua c i c chdng vl slnh v i t t u y i n chgn
Kl hiSu chiing VSV Hogt tinh sinh hgc chlnh Tnri/c ISn men Sau ISn men
Pssnitucillus castaneae S3.1 Mpomyces starkeyi PT5.1 Becaus megaterium P1107
K h i ndng phin giii silk:at (difdng kinh vflng phin giii b$t thuy tmh, mm)
K h i nang smh c h i t gii> I m polysacant {dfl nhfll jy N s/m'}
K h i ning phin glii phit phit khfl lan (dirdng kinh vong phin giii Ca,{PO,),. mm)
K h i ning c6 djnh nito (him lu'gng etylen _hlnh thinh, nmolCiHi/ciy)
37,6x10"" 3 7 0 x 1 0 ' ' 18,0 18,0
K i t q u i B i n g 4 cho I h l y sau qua Irlnh nhSn sinh khfii, hogt linh sinh hgc cua cac chiing vi sinh v^.nghifin c t h i khflng cfl s v thay dfii d i n g k l so vfii gifing gfic. K i t qua trfln chOng to cac thflng sfi ky thu^t li/a chgn phii h e p cho qufl trlnh n h a n smh khfii d c chiing vi sinh v | t
Xac d[nh c h i t mang phCi h g i i
Trong cflng ngh§ san x u l t phan vi slnh v | t , li/a chpn c h i t mang dflng vai tro quan trgng, Snh hudng d i n kha n i n g tfin tgi cua cac chung vi sinh v | t Inang phan vi sinh v | t ; do dfl q u y l l djnh d i n c h l l lugng san f * l m . K l Ihira k i t qua cOa c i c nghifin ci>u tnroc dfiy, chiing ifli da d i n h gia kha nang tfin tgi ciia cac chung vi sinh v§t trong chat mang than bun va tinh bflt s i n + cam ggo. K i t qua duo'c trinh bay Irong Bkng 5.
H O T N G H ! K H O A T I O C CONG NGHE SINFTH^iCTOAN Q U C C 2573
Bang S. Anh hirdng cila loal chat mang vS tho^ gian bao quan din mat dg le b i o vi sinh v.
Kl hi$u chiing VSV
Thdi gian bao quin (thang)
1 2 3 4 5 6
Hoat tfnh smh hgc sau bio quin Chit mang: Than bOn
S3.1 4,2x10° 3,2x10" 3.0x10° 2.4x10° 2,0x10° 1,6x10°
PT5.1 3,2x10' 2,8x10" 2,3x10" 1,3x10" 8.3x10' 1,8x10' P1107 4,6x10" 4,0x10" 3,5x10" 3,2x10" 3,0x10" 2,6x10"
RA18 5.2x10" 4,8x10" 40x10" 3,6x10" 2,4x10" 1,6x10"
Chit mang: Tinh b ^ s i n + cam g^o
Dirdng kinh vdng phin glil silicat, 12,0 mm D$ nhdt 36,6.10-'N.s/m' Dudng kinh vdng phin giii Ca3(POj)j, 18,0 mm Him lugng e^len hlnh thinh, 3.420 nmolCjHi
4,4x10" 4,2x10* 4,0x10' 3,9x10" 3,7x10* 2,4x10" Dudng kfnh vdng^phin giii silicat, 12,0 mm "•
3,5x10" 3,5x10* 3,3x10" 3,1x10* 3,0x10* 2,8x10' Dgnhdl 36,6.10"'N.s/m' S3.1
PT5.1 P1107
FtA18 4,2x10' 4.0x10* 4,0x10' 3,6x10' 3,2x10'
4,6x10' 4,0x10* 3,7x10' 3,5x10* 3,2x10" 3.0x10" Oudng kinh vdng phin glii Ca3{P0,)i. 18,0 mm 2,6x10" Him lugng e^len hlnh thinh,
3.450 nmolCiH.
K i t qua Bang 5 cho t h i y : T r i n n l n c h i t mang than biin, sau 6 thang bao quan, m i l dfi t l b i o chiing S3.1, P1107 va RA18 dgt trfin 10^ CFU/g, mi&t dfi t l b i o chiing PT 5.1 dgt 1,8x10^ CFU/g. Trfin n l n c h l l mang Hnh b^t s i n + cam g?o, sau 6 thang bao quSn, m$t dfi t l bao cac chung vi sinh v§t dgt trfin l O ' CFU/g. Vi v i y , chiing tfli da li/a chgn c h i t mang tinh bfit s i n -t--dm ggo cho-ean'xult phan vi sinh vgL Hogt h'nh sinh hgc ciia cac chiing vi sinh vat thay dfii khflng d i n g k l so vfl'i chOng goc.
x a c d | n h ty' Ig p h d i t r g n d j c h v l s l n h v ^ t v i chat m a n g
T ^ 1$ phfii trfin d j d i vi sinh v§t trong san x u l l phan vi sinh vfit cfl knh hudng Sen mgt dfi vi sinh vfit trong phSn bfln va g i i thanh san p h i m . K i t quS d i n h gia knh hufi'ng cua ty Ifi phfii b^n djch vi slnh v | t den mgt dfi t l bao vl sinh v$t trong c h i t mang sau bao qukn du'g'c t h i h l | n trong Bang 6.
B i n g 6. Anh hud'ng c'ia ty 1$ phfii tr^n djch vl slnh vSt din m | t d$ t i b i o vl slnh v | l trong c h i t mang sau bao quan Ekmvl tinh. CFU/g
Kl hi^u chfinq Ban d i u 1 t h i n g 3 thing ' 6 thing
Tv ii Phfll trdn 5%
S3.1 PT51 P1107 RAI 8
2.2x10°
1.8 X 10°
2.8 X 10°
2.6 X 10"
2,0 X10°
1.2x10°
2,2x10°
1.8x10° -
1,0x10°
4,8 X 10' 1.2x10°
1.1x10°
4,6x10' 2.5x10' 5,8x10' 5 . 0 x 1 0 "
T* IS phdi trgn 10%
S3,1 PT5.1.
P1107 RAI 8
4.6x10"
3 8 x 1 0 ! - . 5.2x10"
5.3 x 10°
4,2x10°
- - 3,5x10°
4,6x10°
4.2x10°
T'» Ifi phdl trdn 15%
S3.1 PT5.1 P1107 RAI 8
5,2x10"
4.2x10"
5.8x10' 5.6x10"
4.6x10°
3.8x10°
5,0x10°
3,8x10°
3,2x10° - 3,7 X 10°
4.4 X 10°
•2,4 X 10°
2,8x10° - - - 3,0x10°
2,6x10°
3,2 X 10°
2,8 X10°
3.8 X10°
3.5x10°
K i t qua Bang 6 cho ttily: Vdri t j Ifi phfii trfin 5%, sau 3 t h i n g b i o quan, mgt dfi tfi bao c i c chOng vi sinh vgt moi logi tning phfin vi sinh v^t khflng dgt theo thflng t u 36/2010/TT-BNNPTNT (mgt dg le bao vi sinh v | t <10^ CFU/g). V 6 i ty ifi phfii trOn 10% va 15%. mfit dfi t l b i o vi sinh v^t mfli logi trong phSn vl sinh v | t dgt >10* CFU/g. Tuy nhifin d l gikm gia manh san p h i m , chung tfll da chgn t j Ifi phfii Irfln 10% cho san x u l t phan vi sinh vgt.
Tir cfic k i t qua trfin, chiing tfli da xSy di/ng qui trinh san x u l t phfln w smh v|it theo so d l 1.
HOI NGHj KHOA HQC C O N G N G H f SINH HQC T O A N Q U 6 C 2013
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HOl NGH! KHOA HQC C O N G NGHE SINH HOG T O A N Q U 6 C 2013
Da li/a chgn dirg^: to h g p vi sinh v$l cho cay Igc trfln dat cat bien gfim chiing cfl kha nang phfin glai silicat (Paenibadllus castaneae S3.1), phan giai phot phat khd lan {Badllus megaterium P1107), cfi dinh nilo cfing sinh cfiy Igc {BradyrhiziMum japonicum RA18) va sinh polysacarit {Lipomyces s^tkeyi PT 5.1).
Oa nghien ci>u xSy d^mg qui trinh s i n x u l t phan vl sinh vat chuyfin dung cho cay Igc trfing tren ^Jat cat b l l n . Phan vl slnh v%t dgt c h l l l i / g n g ttieo thflng lir 36/201 o n T - B N N P T N T (mfit dfi te bao vi sinh v^t mfil loai dgt 10* CFU/g, thfli gian bfio q u i n 6 thang).
TAI U $ U T H A M KHAO
BO Ning n g h l ^ vk Ph5t trien nfing Bion, 2010. Thdng tif 3G/2O-10nT-BtiNPJNT. Quy ^nh sin xuit, kinh doanh vA si> dijng phSn bdn.
Ngfl ThS Dan (chO biSn), 2000. Kf tiiu$t d?t nSng suit lee cao d Vi^t Nam. NhS xult bSn nflng nghiep.
Tfing Kim Thuin, El^ng Thj Mai Anh, Dfi Thj Thu PhiAjng, 2005. NdiHn cdu sin xuit ch4 phim gldimw sinh v$t giir am dit Llpomydn JUL ti> diOng nim tnen Lipomyces stariteyi PT7.1 trdn ea chit bdt sin. Nhihig ^^n dk ca bSn trang nghiSn cuu slnh hgc sir sfing. Bio e&a khoa hgc tgi H^i nghj Toan qufic l l n th>> 5. Dgi hgc Y Md Nfli 3/11/2005. tr. NXB.KHKT.
Phgm V i n Tofin vfl cs, 2005, Ngddn cihi cing ngh$ sin xuit phSn bdn vl slnh v$t da ching mdi. phin bdn ehOc nSng phuc vv ehSm sdc dy tiAng cho mfl s6 vdng dnh tiiai. B^o cflo tfing kfil dfi tai KH d p Nha nirac KC 04.04.
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STUDY ON THE TECHNOLOGY PROCESS OF INOCULANT PRODUCTION FOR GROUNDNUT GROWN AT SANDY SOIL
Nguyen Thu Ha^'and Pham Van T o a n '
'So//s and Fertilizers Research Institute, Vietnam Academy Agricultural Science
^Vleb^am Academy Agricultural Sdence
S U M M A R Y
One combinadon of microorganisms for productioa of tbe inoculant for groimdnut in tasidy soil was selected. It includes silicate bactena {Paenibadllus castaneae S3.1), phosphorous solubilizing bacteria {Badllus megaterium PI 107), nitrogen fixing bacteria {Bradyrhlzobium japonicum RAIS) and polysaccharide synthesized bacteria {Lipomyces starkeyi PT S.I). Technology process of inoculant production for groundnut growing in sandy soil was building. Quality products reach the requirements of Circular 36/20I0/TT-BNNPTNT (density of each effecLve bacteria reach lO' CFU/g, expiry date 6 months).
KeyM-ords: Groundnut, inoculant, nitrogen f m n g hacteria, polysaccharide synthesized tiacteria, phosphoiOOS Solubilizing bacteria, sandy soil, silicate bacteria
'Author for conBSpondenca: Tel: +S4-4-37522125; Email: Hiuhavasi@yahc«.oom
