Efek Ekstrak Etanol Daun Keji Beling (Strobilanthus Crispus (L.) Blume) Terhadap Kontraksi Otot Polos Ileum Marmut Jantan (Cavia Porcellus) Terisolasi Secara Kualitatif

(1)

55

(2)

56

Lampiran 2.

Rekomendasi persetujuan etik penelitian

(3)

57

Lampiran 3.

Gambar tumbuhan keji beling

(

Strobilanthus crispus

L.(Blume))

(4)

58

Gambar daun keji beling

(5)

59

Lampiran 5.

Hasil pemeriksaan mikroskopik penampang melintang serbuk

simplisia daun keji beling

Gambar Mikroskopik serbuk simplisia

Keterangan:

a.

Kutikula

b.

Rambut penutup

c.

Sel litosis dengan sistolit

d.

Epidermis atas

e.

Jaringan palisade

f.

Rambut kelenjar

g.

Jaringan bunga karang

h.

Epidermis bawah

i.

Berkas pembuluh dengan serabut sklerenkim

j.

kolenkim

a

b c

d e f g h

(6)

60

Lampiran 6.

Bagan pembuatan, skrining fitokimia dan karakterisasi serbuk

simplisia

Karakterisasi Simplisia:

- Mikroskopik

- Makroskopik

- Penetapan kadar:

•

air

•

sari yang larut dalam etanol

•

sari yang larut dalam air

•

abu total

•

abu yang tidak larut dalam asam

Skrining Fitokimia:

- Alkaloid

- Flavonoid

- Saponin

- Tanin

- Glikosida

- Antrakuinon

- Steroid/Triterpenoida

Serbuk simplisia daun Keji Beling

Simplisia

Daun Keji Beling

dicuci sampai bersih

dikeringkan di lemari pengering

dengan suhu ± 40

o

C

(7)

61

Lampiran 7.

Bagan pembuatan, skrining fitokimia dan karakterisasi ekstrak

Ekstrak etanol daun Keji Beling

dipekatkan dengan

rotary evaporator

diperkolasi dengan cairan

penyari etanol 96%

Serbuk simplisia daun Keji Beling

Ekstrak Kental

Ekstrak Cair

Skrining Fitokimia:

- Alkaloid

- Flavonoid

- Saponin

- Tanin

- Glikosida

- Antrakuinon

(8)

62

Lampiran 8.

Bagan kerja pengukuran kontraksi ileum marmut terisolasi

Marmut

dislokasi tulang belakang kepala

Preparasi organ

Ekuilibrasi selama 45 menit

(diganti larutan tirode setiap 15 menit)

Tahapan pengujian

Analisis data

Pengujian

kontraksi seri

konsentrasi

Asetilkolin

terhadap otot

polos ileum

Pengujian efek

kontraksi

ekstrak etanol

daun Keji

Beling pada

kontraksi otot

polos ileum

melalui induksi

Asetilkolin

Pengujian

mekanisme aksi

terhadap efek

kontraksi ekstrak

etanol daun Keji

Beling pada otot

polos ileum dengan

(9)

63

Lampiran 9.

Perhitungan kadar air simplisia daun keji beling

% Kadar air simplisia =

volume air (ml )

berat sampel (g)

x 100%

No.

Berat sampel (g)

Volume awal (ml) Volume akhir (ml)

1.

5,045

2,20

2,50

2.

5,012

2,50

2,80

3.

5,030

2,80

3,10

% Kadar air =

volume akhir

−

volume awal

berat sampel

x 100%

a.

Kadar air =

x

100%

5,94%

5,045

2,20

-2,50

=

b.

Kadar air =

x

100%

5,98%

5,012

2,50

-80

2,

=

c.

Kadar air =

x

100%

5

,96%

5,030

2,80

-3,10

=

% Rata-rata kadar air =

5,94% + 5,98% + 5,96%

(10)

64

Lampiran 10.

Perhitungan kadar abu total simplisia daun keji beling

% Kadar abu total =

berat abu (g)

berat sampel (g)

x 100%

No.

Berat sampel (g)

Berat abu (g)

1.

2,0325

0,2075

2.

2,0112

0,2198

3.

2,0084

0,2032

a.

Kadar abu total =

x

100%

10,20%

2,0325

0,2075

=

b.

Kadar abu total =

x

100%

10,92%

2,0112

0,2198

=

c.

Kadar abu total =

x

100%

10,11%

2,0084

0,2032

=

% Rata-rata kadar abu total =

10,20%+ 10,92%+ 10,11%

(11)

65

Lampiran 11.

Perhitungan kadar abu tidak larut asam simplisia daun Keji Beling

% Kadar abu total =

berat abu (g)

berat sampel (g)

x 100%

No.

Berat sampel (g)

Berat abu (g)

1.

2,0325

0,0450

2.

2,0112

0,0412

3.

2,0084

0,0398

a.

Kadar abu total =

x

100%

2,21%

2,0325

0,0450

=

b.

Kadar abu total =

x

100%

2,04%

2,0112

0,0412

=

c.

Kadar abu total =

x

100%

1,96%

2,0205

0,0398

=

% Rata-rata kadar abu total =

2,21%+ 2,04%+ 1,96%

(12)

66

Lampiran 12.

Perhitungan kadar sari larut air simplisia daun Keji Beling

% Kadar sari larut dalam air =

berat sari (g)

berat sampel (g)

x

100

20

x

100%

No.

Berat sampel (g)

Berat sari (g)

1.

5,004

0,183

2.

5,004

0,180

3.

5,005

0,179

a.

Kadar sari larut dalam air=

x

100%

18,28%

20

100

x

5,004

0,183

=

b.

x

100%

17,98%

20

100

x

5,004

0,180

=

c.

x

100%

17,88%

20

100

x

5,005

0,179

=

% Rata-rata kadar sari larut dalam air =

18

,

04

%

3

17,88%

17,98%

18,28%

=

+

(13)

67

Lampiran 13.

Perhitungan kadar sari larut etanol simplisia daun keji beling

% Kadar sari larut dalam etanol =

berat sari (g)

berat sampel (g)

x

100

20

x

100%

No.

Berat sampel (g)

Berat sari (g)

1.

5,010

0,055

2.

5,005

0,057

3.

5,004

0,060

a.

Kadar sari larut dalam etanol =

x

100%

5,48%

20

100

x

5,010

0,055

=

b.

x

100%

5,69%

20

100

x

5,005

0,057

=

c.

x

100%

5,99%

20

100

x

5,004

0,060

=

% Rata-rata kadar sari larut dalam etanol =

5

,

72

%

3

5,99%

5,69%

(14)

68

Konsentrasi

Asetilkol in (M)

1

2

3

4

5

6

1 x 10-8

3,6364

8,3333

3,9216

4,0000

9,0909

5,0000

5,6166

1,0072

3 x 10-8

6,1818

12,5000

5,8824

6,8000

13,6364

7,5000

8,7501

1,3919

1 x 10-7

7,2727

12,5000

7,8431

8,0000

13,6364

10,0000

9,8754

1,0873

3 x 10-7

10,9091

20,8333

11,7647

12,0000

22,7273

15,0000

15,5391

2,0671

1 x 10-6

18,1818

41,6667

15,6863

20,0000

36,3636

20,0000

25,3164

4,4320

3 x 10-6

29,0909

54,1667

19,6078

32,0000

40,9091

25,0000

33,4624

5,0627

1 x 10-5

32,7273

75,0000

54,9020

52,0000

63,6364

50,0000

54,7110

5,7855

3 x 10-5

76,3636

87,5000

86,2745

56,0000

63,6364

60,0000

71,6291

5,5742

1 x 10-4

87,2727

93,7500

94,1176

72,0000

72,7273

65,0000

80,8113

5,0964

3 x 10-4

92,7273

95,8333

96,0784

80,0000

81,8182

80,0000

87,7429

3,2392

1 x 10-3

94,5455

95,8333

98,0392

92,0000

90,9091

90,0000

93,5545

1,2693

3 x 10-3

100,0000 100,0000 100,0000 100,0000 100,0000 100,0000

100,0000

0,0000

% Kontraksi Ileum*

Rerata

SEM

Lampiran 14.

Data pengujian kontraksi seri konsentrasi asetilkolin terhadap otot polos ileum

1.

Tabel data pengujian kontraksi seri konsentrasi asetilkolin terhadap otot polos ileum

Keterangan :

*

= %kontraksi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin

(15)

69

Lampiran 14.

(Lanjutan)

2.

Hitungan EC80 asetilkolin

��

80

=

�

80−�1

�2−�1

× (

�

2

− �

1

)

�

+

�

1

Keterangan:

X

1

: Log. konsentrasi dengan respons tepat di bawah 80%

X

2

: Log. konsentrasi dengan respons tepat di atas 80%

Y

1

: %respons tepat di bawah 80%

Y

2

: %respons tepat di atas 80%

�

.

��

80

=

�

80

−

76,3636

87,2727

−

76,3636

×

�−

4

−

(

−

4,5)

��

+ (

−

4,5)

��

₈₀

=

−

4,3333

EC

80

= 4,64 x 10

-5

M

�

.

��

80

=

�

80

−

75,0000

87,5000

−

75,0000

×

�−

4,5

−

(

−

5)

��

+ (

−

5, )

��

80

=

−

4,8

EC

80

= 1,58 x 10

-5

M

�

.

��

₈₀

=

�

80

−

54,9020

86,2745

−

54,9020

×

�−

4,5

−

(

−

5)

��

+ (

−

5)

��

₈₀

=

−

4,6

EC

80

= 2,51 x 10

-5

M

�

.

��

80

=

�

80

−

72,0000

80,000

−

72,0000

×

�−

3,5

−

(

−

4)

��

+ (

−

4)

��

₈₀

=

−

4

EC

80

= 1 x 10

-4

M

�

.

��

80

=

�

80

−

72,7273

81,8182

−

72,7273

×

�−

3,5

−

(

−

4)

��

+ (

−

4)

��

₈₀

=

−

3,6

EC

80

= 2,51 x 10

-4

M

�

.

��

₈₀

=

�

80

−

65,0000

80

−

65,0000

×

�−

3,5

−

(

−

4)

��

+ (

−

4)

��

80

=

−

4

(16)

70

Rerata EC

80

asetilkolin

=

4,64

�

10

−5

+ 1,58

�

10

−5

+ 2,51

�

10

−5

+ 1

�

10

−4

+2,51

�

10

−4

+1

�

10

−4

6

(17)

71

Lampiran 15.

(Lanjutan)

3.

Hitungan EC80 ekstrak

��

80

=

�

80−�1

�2−�1

× (

�

2

− �

1

)

�

+

�

1

Keterangan:

X

1

: Log. konsentrasi dengan respons tepat di bawah 80%

X

2

: Log. konsentrasi dengan respons tepat di atas 80%

Y

1

: %respons tepat di bawah 80%

Y

2

: %respons tepat di atas 80%

�

.

��

80

=

�

80

−

54,5455

81,8182

−

54,5455

×

�

0,8450

−

(0,7781)

��

+ (0,7781)

��

80

= 0,7825

EC

80

= 6,0603 M

�

.

��

80

=

�

80

−

50,0000

83,3333

−

50,0000

×

�

0,8450

−

(0,7781)

��

+ (0,7781)

��

₈₀

= 0,7825

EC

80

= 6,0603 M

�

.

��

₈₀

=

�

80

−

45,4445

81,8182

−

45,4445

×

�

0,8450

−

(0,7781)

��

+ (0,7781)

��

80

= 0,7825

EC

80

= 6,0603 M

�

.

��

80

=

�

80

−

69,1538

84,6154

−

69,1538

×

�

0,8450

−

(0,7781)

��

+ (0,7781)

��

80

= 0,7825

EC

80

= 6,0603 M

�

.

��

₈₀

=

�

80

−

58,8235

82,3529

−

58,8235

×

�

0,8450

−

(0,7781)

��

+ (0,7781)

��

80

= 0,7825

EC

80

= 6,0603 M

�

.

��

80

=

�

80

−

52,7778

83,3333

−

52,7778

×

�

0,8450

−

(0,7781)

��

+ (0,7781)

��

80

= 0,7825

EC

80

= 6,0603 M

Rerata EC

80

EEDKB =

6,0603+6,0603+6,0603+6,0603+6,0603+6,0603

6

(18)

73

Lampiran 15.

Data pengujian efek kontraksi ekstrak etanol daun Keji Beling pada kontraksi ileum

Dosis Ekstrak

Rerata

SEM

(mg/ml)

1

2

3

4

5

3

1,0

0,0000

8,3333

0,0000

7,6923

5,8824

3,6513

1,6656

2,0

0,0000

8,3333

9,0909

0,0000

7,6923

5,8824

5,1665

1,6902

3,0

9,0909

8,3333

18,1818

5,5556

15,3846

8,8235

11,0313

1,9449

4,0

18,1818

12,5000

18,1818

13,8889

38,4615

29,4118

21,7710

4,1263

5,0

27,2727

25,0000

40,9091

22,2222

46,1538

52,9412

35,7498

5,1666

6,0

54,5455

50,0000

45,4454

52,7778

69,1538

58,8235

55,1243

3,3481

7,0

81,8182

83,3333

81,8182

83,3333

84,6154

82,3529

82,8786

0,4455

8,0

100,0000 100,0000 100,0000 100,0000

100,0000

100,0000 100,0000

0,0000

% Kontraksi Ileum*

Keterangan :

(19)

74

Lampiran 16.

Data efek kontraksi ekstrak etanol daun Keji Beling terhadap kontraksi otot polos ileum dengan inkubasi awal menggunakan

atropin sulfat selama 10 menit

Dosis Ekstrak

(mg/ml)

1

2

3

4

5

6

1,0

0,0000

2,0

0,0000

3,0

7,6923

11,1111

12,5000

0,0000

10,0000

8,3333

8,2728

1,8045

4,0

7,6923

22,2222

25,0000

18,1818

20,0000

25,0000

19,6827

2,6401

5,0

23,0769

44,4444

25,0000

36,3636

30,0000

33,3333

32,0364

3,2057

6,0

53,8462

55,5556

50,0000

63,6364

70,0000

66,6667

59,9508

3,2410

7,0

84,6154

83,3333

81,2500

81,8182

80,0000

83,3333

82,3917

0,6848

8,0

100,0000

100,0000 100,0000 100,0000 100,0000 100,0000 100,0000

0,0000

Rerata

SEM

% Kontraksi Ileum*

Keterangan :

(20)

75

Lampiran 17.

Data AUC efek kontraksi ekstrak etanol daun Keji Beling pada kontraksi ileum

1

2

3

4

5

6

7

1,0

0,0000

2,4773

7,0227

11,3636

20,4546

34,0909

45,4546 120,8636

2,0

4,1667

5,2083

9,3750

18,7500

33,3333

45,8333 120,8333

3,0

2,2727

6,8182

9,0909

14,7727

21,5886

31,8159

45,4546 131,8136

4,0

0,0000

1,3889

4,8611

9,0278

18,7500

34,0278

45,8333 113,8889

5,0

3,8462

5,7692

13,4615

21,1538

28,8269

38,4423

46,1539 157,6538

6,0

2,9412

3,6765

9,5588

20,5883

27,9412

35,2941

46,1539 145,5883

Rerata

2,2045

4,0494

8,2006

14,3802

22,7185

34,5007

45,7196 131,7736

SEM

0,7486

0,8231

1,3141

2,2164

1,8483

0,9152

0,1114

6,8780

Total

AUC*

Marmut

Keterangan :

(21)

76

Lampiran 18.

Data AUC efek kontraksi ekstrak etanol daun Keji Beling terhadap kontraksi otot polos ileum dengan inkubasi awal

menggunakan atropin sulfat selama 10 menit

1

2

3

4

5

6

7

1,0

0,0000

1,9231

3,8462

7,6923

19,2308

34,6154

46,1539 113,4616

2,0

0,0000

2,7778

8,3333

16,6667

25,0000

34,7222

45,8333 133,3333

3,0

0,0000

3,1250

9,3550

12,5000

18,7500

32,8125

45,3125 121,8750

4,0

0,0000

4,5455

13,6364

25,0000

36,3637

45,4546 125,0000

5,0

0,0000

2,5000

7,5000

12,5000

25,0000

37,5000

45,0000 130,0000

6,0

0,0000

2,0833

8,3333

14,5833

25,0000

37,5000

45,8333 133,3333

Rerata

0,0000

2,0682

6,9889

12,9298

22,9968

35,5856

45,5979 126,1672

SEM

0,0000

0,4511

0,9205

1,2247

1,2685

0,7596

0,1712

3,1550

Total

AUC*

Marmut

Keterangan :

(22)

77

Lampiran 19.

Hasil uji korelasi efek kontraksi ekstrak etanol daun Keji Beling

pada kontraksi ileum

Descriptive Statistics

Mean Std. Deviation N

Dosis 4.50 2.449 8

% Kontraksi 40.872550 37.7290901 8

Correlations

Dosis % Kontraksi

Dosis Pearson Correlation 1 .965**

Sig. (2-tailed) .000

N 8 8

% Kontraksi Pearson Correlation .965** 1

Sig. (2-tailed) .000

N 8 8

**. Correlation is significant at the 0.01 level (2-tailed).

Model Summary

Model R R Square

Adjusted R

Square

Std. Error of the

Estimate

1 .965a .932 .921 10.6129278

(23)

78

Lampiran 20.

Hasil uji-t independen nilai %kontraksi ekstrak etanol daun Keji

Beling 6 mg/ml dengan nilai %kontraksi asetilkolin 1 x 10

-4

M

Descriptives

Perlakuan Statistic Std. Error

% Kontraksi ACh Mean 55.107667 3.3487096

95% Confidence Interval for

Mean

Lower Bound 46.499535

Upper Bound 63.715799

5% Trimmed Mean 54.864119

Median 53.611650

Variance 67.283

Std. Deviation 8.2026298

Minimum 45.4454

Maximum 69.1538

Range 23.7084

Interquartile Range 12.5447

Skewness .964 .845

Kurtosis 1.256 1.741

Ekstrak Mean 80.811267 5.0964188

Mean

Median 80.000000

Variance 155.841

Minimum 65.0000

Maximum 94.1176

Range 29.1176

Skewness -.058 .845

(24)

79

Lampiran 20.

(Lanjutan)

Tests of Normality

Perlakuan

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

% Kontraksi ACh .199 6 .200* .949 6 .732

Ekstrak .241 6 .200* .875 6 .247

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.

Group Statistics

Perlakuan N Mean Std. Deviation Std. Error Mean

% Kontraksi ACh 6 55.107667 8.2026298 3.3487096

Ekstrak 6 80.811267 12.4836256 5.0964188

Independent Samples Test

Levene's Test for Equality of

Variances t-test for Equality of Means

95% Confidence Interval of the Difference

F Sig. t df Sig. (2-tailed) Mean Difference Std. Error

Difference Lower Upper

% Kontraksi

Equal variances Assumed

3.874 .077 -4.215 10 .002 -25.7036000 6.0981424 -39.2911080 -12.1160920

Equal variances not assumed

(25)

80

Lampiran 21.

Hasil uji-t independen nilai %kontraksi ekstrak etanol daun Keji

Beling terhadap kontraksi otot polos ileum dengan inkubasi

larutan atropin sulfat dan tanpa inkubasi

Descriptivesa,b,c,d

1 mg/mL Ekstrak Mean 3.651333 1.6655789

Mean

Lower Bound -.630173

Median 2.941200

Variance 16.645

Minimum .0000

Maximum 8.3333

Range 8.3333

Skewness .149 .845

Kurtosis -2.952 1.741

Mean

Lower Bound .821588

Median 6.787350

Variance 17.141

Minimum .0000

Maximum 9.0909

Range 9.0909

Skewness -.707 .845

Kurtosis -1.953 1.741

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81

Mean Upper Bound 15.946898

Median 8.957200

Variance 23.174

Minimum 5.5556

Maximum 18.1818

Range 12.6262

Skewness .790 .845

Kurtosis -.892 1.741

Atropin+Ekstrak Mean 8.272783 1.8045151

95% Confidence Interval

for Mean

Median 9.166650

Variance 19.538

Minimum .0000

Maximum 12.5000

Range 12.5000

Skewness -1.608 .845

Mean

Median 18.181800

Variance 102.160

Minimum 12.5000

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82

Range 25.9615

Skewness 1.080 .845

Mean

Median 21.111100

Variance 41.824

Minimum 7.6923

Maximum 25.0000

Range 17.3077

Skewness -1.564 .845

Mean

Median 34.090900

Variance 160.164

Minimum 22.2222

Maximum 52.9412

Range 30.7190

Skewness .299 .845

Kurtosis -2.108 1.741

Mean

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83

Median 31.666650

Variance 61.659

Minimum 23.0769

Maximum 44.4444

Range 21.3675

Skewness .569 .845

Mean

Median 67.424250

Variance 55.387

Minimum 52.7778

Maximum 72.7273

Range 19.9495

Skewness -.917 .845

for Mean

Median 59.596000

Variance 63.055

Minimum 50.0000

Maximum 70.0000

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84

Skewness .042 .845

Kurtosis -2.027 1.741

for Mean

Median 83.974350

Variance 13.271

Minimum 81.8182

Maximum 91.6667

Range 9.8485

Skewness 1.479 .845

Atropin+Ekstrak Mean 82.391700 .6848521

for Mean

Median 82.575750

Variance 2.814

Minimum 80.0000

Maximum 84.6154

Range 4.6154

Skewness -.171 .845

a. 1 mg/mL is constant when Perlakuan = Atropin+Ekstrak. It has been omitted.

b. 2 mg/mL is constant when Perlakuan = Atropin+Ekstrak. It has been omitted.

c. 8 mg/mL is constant when Perlakuan = Ekstrak. It has been omitted.

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85

Lampiran 21.

(Lanjutan)

Tests of Normalityb,c,d,e

Perlakuan

1 mg/mL Ekstrak .315 6 .064 .776 6 .035

2 mg/mL Ekstrak .235 6 .200* .812 6 .076

3 mg/mL Ekstrak .313 6 .068 .884 6 .288

Atropin+Ekstrak .281 6 .150 .856 6 .176

4 mg/mL Ekstrak .305 6 .084 .867 6 .216

Atropin+Ekstrak .242 6 .200* .838 6 .126

5 mg/mL Ekstrak .249 6 .200* .903 6 .393

Atropin+Ekstrak .148 6 .200* .962 6 .836

6 mg/mL Ekstrak .270 6 .197 .908 6 .426

Atropin+Ekstrak .210 6 .200* .935 6 .621

7 mg/mL Ekstrak .211 6 .200* .864 6 .203

Atropin+Ekstrak .213 6 .200* .966 6 .862

p*. This is a lower bound of the true significance.

b. 1 mg/mL is constant when Perlakuan = Atropin+Ekstrak. It has been omitted.

c. 2 mg/mL is constant when Perlakuan = Atropin+Ekstrak. It has been omitted.

d. 8 mg/mL is constant when Perlakuan = Ekstrak. It has been omitted.

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86

Lampiran 21.

(Lanjutan)

Group Statistics

1 mg/mL Ekstrak 6 3.651333 4.0798183 1.6655789

Atropin+Ekstrak 6 .000000 .0000000 .0000000

2 mg/mL Ekstrak 6 5.166483 4.1402213 1.6902383

Atropin+Ekstrak 6 .000000 .0000000 .0000000

3 mg/mL Ekstrak 6 10.894950 4.8139662 1.9652935

Atropin+Ekstrak 6 8.272783 4.4201413 1.8045151

4 mg/mL Ekstrak 6 21.770967 10.1074059 4.1263312

Atropin+Ekstrak 6 19.682717 6.4671399 2.6401988

5 mg/mL Ekstrak 6 35.749833 12.6556012 5.1666276

Atropin+Ekstrak 6 32.036367 7.8523317 3.2057010

6 mg/mL Ekstrak 6 64.721817 7.4422304 3.0382779

Atropin+Ekstrak 6 59.950817 7.9407270 3.2417882

7 mg/mL Ekstrak 6 85.025017 3.6430025 1.4872495

Atropin+Ekstrak 6 82.391700 1.6775382 .6848521

8 mg/mL Ekstrak 6 100.00000 .000000a .000000

Atropin+Ekstrak 6 100.00000 .000000a .000000

a. t cannot be computed because the standard deviations of both groups are 0.

Independent Samples Test Levene's

Test for Equality of

F Sig. T df Sig. (2-tailed) Mean Difference Std. Error

Difference Lower Upper 1 mg/mL Equal variances assumed 123.78 4

.000 2.192 10 .053 3.6513333 1.6655789 -.0598076 7.3624743

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87

Lampiran 21.

(Lanjutan)

2 mg/mL

Equal variances assumed

24.499 .001 .3.057 10 .012 5.1664833 1.6902383 1.4003978 8.9325689

.3.057 5.000 .028 5.1664833 1.6902383 .8215876 9.5113791

3 mg/mL

.414 .535 .983 10 .349 2.6221667 2.6680804 -3.3226870 8.5670204

983 9.928 .349 2.6221667 2.6680804 -3.3285338 8.5728672

4 mg/mL

1.921 .196 .426 10 .679 2.0881500 4.8986997 -8.8267331 13.0032331

.426 8.506 .680 2.0881500 4.8986997 -9.0922006 13.2687006

5 mg/mL

4.080 .071 .611 10 .555 3.7134667 6.0803420 -9.8343796 17.2613130

.611 8.353 .558 3.7134667 6.0803420 -10.2053991 17.6323324

6 mg/mL

.224 .464 .611 10 .308 4.7710000 4.4430083 -5.1286395 14.6706395

.611 8.353 .308 4.7710000 4.4430083 -5.1342672 14.6762672

7 mg/mL

1.871 .201 1.608 10 .139 2.6333167 1.6373557 -1.0149391 6.2815724

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88

Lampiran 22.

Hasil uji-t independen nilai AUC dari %kontraksi ekstrak etanol

daun keji beling dengan inkubasi atropin sulfat dan tanpa inkubasi

Descriptives

AUC Ekstrak Mean 131.773583 6.8780158

Mean

Median 126.338600

Variance 283.843

Minimum 113.8889

Maximum 157.6538

Range 43.7649

Skewness .730 .845

Mean

Median 127.500000

Variance 59.723

Minimum 113.4616

Maximum 133.3333

Range 19.8717

Skewness -.871 .845

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89

Lampiran 22.

(Lanjutan)

Tests of Normality

Perlakuan

AUC Ekstrak .241 6 .200* .916 6 .477

Atropin+Ekstrak .190 6 .200* .905 6 .401

AUC Ekstrak 6 131.773583 16.8476292 6.8780158

Atropin+Ekstrak 6 126.167200 7.7280520 3.1549640

Independent Samples Test

F Sig. T Df Sig.

(2-tailed)

Mean Difference

Std. Error

AUC Equal variances assumed

3.483 .092 .741 10 .476 5.6063833 7.5670932 -11.2541510 22.4669177

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90

Lampiran 2.3

Hasil uji-t independen nilai dari kekuatan kontraksi ekstrak etanol

daun keji beling dengan asetilkolin

Descriptives

% Kontraksi ACh Mean .481250 .1439052

Mean

Lower Bound .140968

Upper Bound .821532

5% Trimmed Mean .462500

Median .350000

Variance .166

Std. Deviation .4070254

Minimum .1000

Maximum 1.2000

Range 1.1000

Interquartile Range .7125

Skewness 1.103 .752

Ekstrak Mean .731250 .2185458

Mean

Lower Bound .214471

5% Trimmed Mean .712500

Median .700000

Variance .382

Std. Deviation .6181409

Minimum .1000

Maximum 1.7000

Range 1.6000

Skewness .420 .752

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91

Tests of Normality

Perlakuan

% Kontraksi ACh .232 8 .200* .850 8 .094

Ekstrak .201 8 .200* .899 8 .283

% Kontraksi ACh 8 .481250 .4070254 .1439052

Ekstrak 8 .731250 .6181409 .2185458

Independent Samples Test

F Sig. T Df Sig.

(2-tailed)

Mean Difference

Std. Error

AUC Equal variances assumed

2.629 .127 -.955 14 .356 -.2500000 .2616696 -.8112255 .3112255

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92

Lampiran 24.

Gambar preparasi organ ileum marmut

A

B

C

Keterangan:

A = pemotongan abdomen marmut

B = organ ileum marmut

C = proses pengikatan organ ileum marmut terisolasi

ileum

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93

Lampiran 25.

Gambar alat

organ bath

A

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94

Lampiran 25.

(Lanjutan)

C

D

Keterangan:

A = empat set

organ bath

volume 50,0 ml C = transduser isometrik

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95

Lampiran 26.

Gambar pola kontraksi ileum marmut terisolasi

Gambar a. Pola kontraksi otot polos organ ileum terisolasi yang dikontraksi

dengan pemberian seri konsentrasi asetilkolin (10

-8

– 3 x 10

-3

M).

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96

Lampiran 26.

(Lanjutan)