رﻮﭙﺳا مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
ﻲﮔدﻮﻟآ و
ناﺮﻳا يﺪﻴﻟﻮﺗ يﺎﻫ ﻞﺴﻋ رد ﻲﭼرﺎﻗ
نﺎﮔﺪﻨﺴﻳﻮﻧ :
يﺪﻤﺤﻣ ﻲﺟﺎﺣ ردﺎﻬﺑ ﻲﻧﺎﻘﻫد ﻲﻠﻋ ،
1هدازداﻮﺟ ﻲﻀﺗﺮﻣ ،
2،
3ﺮﻓ ﻲﺘﻌﻳﺮﺷ ﻲﺒﻧ
،
4ﺎﮕﻨﻫ
ﻣ يﺪﻧز ﻪ يوﺮﺴﺧ يﺮﻔﻈﻣ ﻦﺴﺣ ،
5،
6ﻲﻣﻼﺳا اﺪﻠﻴﮔ
7
.1 رﺎﻳدﺎﺘﺳا ،اﺬﻏ ﻲﻨﻤﻳا و ﺖﻣﻼﺳ تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ هﺪﻜﺸﻧاد
،ﺖﺷاﺪﻬﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و
ﺪﻴﻬﺷ
دﺰﻳ ﻲﻗوﺪﺻ .2 رﺎﻳدﺎﺘﺳا هوﺮﮔ ﭘا ﻴ ﻣﺪ ﻴ ژﻮﻟﻮ ي
، هﺪﻜﺸﻧاد
،ﺖﺷاﺪﻬﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و
دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ
.3 لﻮﺌﺴﻣ هﺪﻨﺴﻳﻮﻧ :
يﻮﺠﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد،ﻲﻳاﺬﻏ داﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
تﺎﻣﺪﺧ و
ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ
سﺎﻤﺗ ﻦﻔﻠﺗ : 09159348715 Email:[email protected]
4 . دﺎﺘﺳا ﻳ رﺎ ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ،ﻂﻴﺤﻣ ﺖﺷاﺪﻬﺑ ﻲﺳﺪﻨﻬﻣ هوﺮﮔ
5 . رﺎﻳدﺎﺘﺳا هوﺮﮔ ﻣ ﻲﺳﺎﻨﺷ بوﺮﻜﻴ
، هﺪﻜﺸﻧاد ﻜﺷﺰﭘ ﻲ
، ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و
دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ
6 . دﺎﺘﺳا هوﺮﮔ ﺬﻐﺗ ﻳ
،ﻪ هﺪﻜﺸﻧاد
،ﺖﺷاﺪﻬﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و
دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ
7 . رﺎﻳدﺎﺘﺳا هوﺮﮔ ﻞﮕﻧا ﺳﺎﻨﺷ ﻲﺳﺎﻨﺷ چرﺎﻗ و ﻲ
، هﺪﻜﺸﻧاد ﻜﺷﺰﭘ ﻲ
، ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و
ﺪﻴﻬﺷ
دﺰﻳ ﻲﻗوﺪﺻ
ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ ود دﺰﻳ ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ﻢﻫدﺰﻧﺎﭘ لﺎﺳ
هرﺎﻤﺷ : ﻢﺸﺷ
ﺪﻨﻔﺳا و ﻦﻤﻬﺑ 1395
ﻞﺴﻠﺴﻣ هرﺎﻤﺷ 60 :
لﻮﺻو ﺦﻳرﺎﺗ :
2 / 2 / 1393
شﺮﻳﺬﭘ ﺦﻳرﺎﺗ :
5 / 3 / 1393
ﻲﺸﻫوﮋﭘ ﻪﻟﺎﻘﻣ
هﺪﻴﻜﭼ
ﻪﻣﺪﻘﻣ ﻞﺴﻋ: هدﺎﻣ ﻲﻳاﺬﻏ رﺎﻴﺴﺑ ﻲﻨﻏ و يژﺮﻧا ﺶﺨﺑ ﺖﺳا ﻞﺴﻋ رﻮﺒﻧز ﻂﺳﻮﺗ نﺎﻫﺎﻴﮔ ﺪﻬﺷ زا ﻞﺻﺎﺣ .
ﻞﺴﻋ رد
دراد دﻮﺟو رﻮﭙﺳا ﻞﻜﺷ ﻪﺑ ﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ دﻮﺟو لﺎﻤﺘﺣا .
يﺮﺘﻛﺎﺑ زﺎﺳﺮﻄﺧ يﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﻦﻳا زا ﻲﻜﻳ
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ دراد ار نادازﻮﻧ رد هﮋﻳو ﻪﺑ ﻢﺴﻴﻟﻮﺗﻮﺑ زوﺮﺑ ﻲﻳﺎﻧاﻮﺗ ﻪﻛ ﺖﺳا
. ﻲﺳرﺮﺑ رﻮﻈﻨﻣ ﻪﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳا
ﻮﻴﺷ ناﺰﻴﻣ رﻮﭙﺳا ﻪﺑ ناﺮﻳا ﻒﻠﺘﺨﻣ ﻖﻃﺎﻨﻣ يﺪﻴﻟﻮﺗ يﺎﻫ ﻞﺴﻋ ﻲﮔدﻮﻟآ ع مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
ﺮﻤﺨﻣ و ﻚﭙﻛ و
ﺖﻓﺮﮔ ترﻮﺻ .
شور ﺳرﺮﺑ ﻲ لﺎﺳ رد ﻲﻌﻄﻘﻣ ﻲﻔﻴﺻﻮﺗ ﺔﻌﻟﺎﻄﻣ ﻦﻳا: 1392
داﺪﻌﺗ يور ﺮﺑ 130
زا هﺪﺷ ﻪﻴﻬﺗ ،ﻞﺴﻋ ﻪﻧﻮﻤﻧ
يﺎﻫ نﺎﺘﺳرﻮﺒﻧز ﺖﻓﺮﮔ مﺎﺠﻧا رﻮﺸﻛ
. رﻮﭙﺳا ﻲﻧاواﺮﻓ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
ﻞﺴﻋ رد زا هدﺎﻔﺘﺳا ﺎﺑ ناﺮﻳا يﺎﻫ
تﺪﻣ ﻪﺑ يراﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ هﺪﺷ ﻪﺘﺨﭘ ﺖﺷﻮﮔ ﺖﺸﻛ ﻂﻴﺤﻣ و ﻲﺗراﺮﺣ كﻮﺷ 10
يﺎﻣد رد زور 35
ﻲﺘﻧﺎﺳ ﻪﺟرد
ﺖﻓﺮﮔ راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ داﺮﮔ .
ﺲﻧﮋﻧﺮﻓﺮﭘ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ يﺮﺘﻛﺎﺑ PTCC:1766
ﺖﺒﺜﻣ لﺮﺘﻨﻛ ي ﻪﻧﻮﻤﻧ ناﻮﻨﻋ ﻪﺑ
ﺪﺷ هدﺎﻔﺘﺳا . ﻣ دراﺪﻧﺎﺘﺳا ﻖﺒﻃ ﺰﻴﻧ ﺮﻤﺨﻣ و ﻚﭙﻛ شرﺎﻤﺷ نﻮﻣزآ ﺖﻓﺮﻳﺬﭘ مﺎﺠﻧا ناﺮﻳا ﻲﻠ
.
ﻪﺘﻓﺎﻳ ﺎﻫ رﻮﭙﺳا داد نﺎﺸﻧ ﺞﻳﺎﺘﻧ: مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
ﺖﺷاﺪﻧ رﻮﻀﺣ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ ﻪﻧﻮﻤﻧ زا ﻚﻳ ﭻﻴﻫ رد .
رد ﺮﻤﺨﻣ و ﻚﭙﻛ ﻪﺑ ﻲﮔدﻮﻟآ 34
ﻪﻧﻮﻤﻧ ) 67 / 26 (%
دﻮﺑ دراﺪﻧﺎﺘﺳا زﺎﺠﻣ ﺪﺣ زا ﺮﺗﻻﺎﺑ .
ﺎﺑ نﺎﺘﺳدﺮﻛ نﺎﺘﺳا 6
/ 66 %
ﻦﻳﺮﺘﺸﻴﺑ ،زﺎﺠﻣ ﺪﺣ زا ﺮﺗﻻﺎﺑ ﻲﮔدﻮﻟآ ﻲﮔدﻮﻟآ ﻪﻨﻣاد ﺎﺑ نارﺪﻧزﺎﻣ و ﻲﮔدﻮﻟآ
CFU/g 55 - 33 ناﺰﻴﻣ ﻦﻳﺮﺘﻤﻛ
ﺪﻧدﻮﺑ اراد ﺮﻤﺨﻣ و ﻚﭙﻛ ﻪﺑ ﻲﮔدﻮﻟآ .
ﻪﺠﻴﺘﻧ ﺮﻴﮔ ي رﻮﭙﺳا ﺮﻈﻧ زا ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﻲﻧﺎﻣز ﻊﻄﻘﻣ رد ناﺮﻳا يﺎﻫ ﻞﺴﻋ: مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
هدﻮﺑ ﻦﻤﻳا
ﺎﻣا ﻲﮔدﻮﻟآ ﻚﭙﻛ ﻪﺑ يﺎﻫ ﻞﺴﻋ رد ﺮﻤﺨﻣ و ﻖﻃﺎﻨﻣ
رﻮﺸﻛ بﺮﻏ
رﻮﺸﻛ لﺎﻤﺷ و ﺮﺘﺸﻴﺑ
ﻦﻳﺮﺘﻤﻛ ار ناﺰﻴﻣ اراد
ﻲﻣ ﺪﺷﺎﺑ .
هژاو يﺪﻴﻠﻛ يﺎﻫ ،ﻞﺴﻋ:
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ ﻲﻳاﺬﻏ داﻮﻣ ﻲﻨﻤﻳا ،چرﺎﻗ ،
ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ ﻪﻣﺎﻧ نﺎﻳﺎﭘ زا ﻪﺘﻓﺮﮔ ﺮﺑ ﻪﻟﺎﻘﻣ ﻦﻳا ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد،ﻲﻳاﺬﻏ داﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ
و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ دﺰﻳ ﻲﻗوﺪﺻ
ﺪﺷﺎﺑ ﻲﻣ .
ﻞﺴﻋ هدﺎﻣ ﻲﻳاﺬﻏ رﺎﻴﺴﺑ ﻲﻨﻏ و يژﺮﻧا ﺶﺨﺑ
، ﺪﺿ ﺖﻴﺻﺎﺧ ﺎﺑ
ﺖﺳا ﻞﺴﻋ رﻮﺒﻧز ﻂﺳﻮﺗ نﺎﻫﺎﻴﮔ ﺪﻬﺷ ﻆﻴﻠﻐﺗ زا ﻞﺻﺎﺣ ﻲﺑوﺮﻜﻴﻣ .
ﻪﺑ ﻪﻛ هدﻮﺑ ﻞﺴﻋ هﺪﻨﻫد ﻞﻴﻜﺸﺗ تﺎﺒﻴﻛﺮﺗ ﻦﻳﺮﺗ هﺪﻤﻋ بآ و ﺪﻨﻗ ﺮﺑاﺮﺑ ﺐﻴﺗﺮﺗ 80
% و 17 ﺎﺗ 18 % ﺪﻨﺷﺎﺑ ﻲﻣ . رد دﻮﺟﻮﻣ يﺎﻫ ﺪﻨﻗ
زﻮﺘﻛوﺮﻓ ﻞﺴﻋ
) 19 / 38 (%
ﺰﻛﻮﻠﮔ ، ) 28 / 31 (%
زرﺎﻛﺎﺳ ، ) 3 / 1 (%
و
زﻮﺘﻟﺎﻣ ) 3 / 7 (%
ﺖﺳا . ﻪﻛ ﺎﻫﺪﻴﺳا ﻞﻣﺎﺷ ﻞﺴﻋ رد دﻮﺟﻮﻣ داﻮﻣ ﺮﻳﺎﺳ
دوﺪﺣ رد ﺪﺷﺎﺑ ﻲﻣ ﻚﻴﻣﺮﻓ ﺪﻴﺳا ﺎﺒﻟﺎﻏ 57
/ 0
% ﻦﻴﺌﺗوﺮﭘ ، 226
/ 0 ،
نژوﺮﺘﻴﻧ 043 / 0
% ﺪﻴﺳاﻮﻨﻴﻣآ ، 1
/ 0
%
، دوﺪﺣ رد ﻲﻧﺪﻌﻣ داﻮﻣ 17
/ 0
%
و ،ﻚﻴﺗﺎﻣورآ داﻮﻣ ،ﺎﻫ ﻪﻧاﺪﮕﻧر ﻞﺜﻣ ،ﻞﺴﻋ رد دﻮﺟﻮﻣ داﻮﻣ ﺮﮕﻳد ﻪﻛ ﺎﻫ ﻦﻴﻣﺎﺘﻳو و ﻲﻠﻜﻟا يﺎﻫﺪﻨﻗ ،يﺪﻴﺋﻮﻠﻛ تارذ ،ﻲﻠﻨﻓ تﺎﺒﻴﻛﺮﺗ
دوﺪﺣ رد ﺎﻋﻮﻤﺠﻣ 1
/ 2
%
ﻞﻣﺎﺷ ار ﻞﺴﻋ ﻞﻛ تﺎﺒﻴﻛﺮﺗ زا
ﺪﻧﻮﺷ ﻲﻣ ) 1 ( .
رﺎﺸﻓ ،ﻦﻴﻳﺎﭘ ﻲﺑآ ﺖﻴﻟﺎﻌﻓ ﻞﻴﺒﻗ زا ﻞﺴﻋ ﻲﻧورد يﺎﻫ ﻪﺼﺨﺸﻣ ،ﻻﺎﺑ يﺰﻤﺳا ﻮﺗ ،ﻦﻴﻳﺎﭘpH
ﺪﺿ تﺎﺒﻴﻛﺮﺗ و نژورﺪﻴﻫ ﺪﻴﺴﻛوﺮﭘ ﺪﻴﻟ
ﺮﺘﻤﻛ ﻲﻌﻴﺒﻃ ﻞﺴﻋ رد ﺎﺗ دﻮﺷ ﻲﻣ ﺚﻋﺎﺑ ﻲﻌﻴﺒﻃ ﻲﺑوﺮﻜﻴﻣ
ﺪﻧﺎﻤﺑ ﻲﻗﺎﺑ لﺎﻌﻓ ترﻮﺻ ﻪﺑ ﻲﻤﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ )
4 - 2 ( . ﻞﺴﻋ رد
ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﻲﻟو دراﺪﻧ دﻮﺟو يا هﺪﻧز ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﭻﻴﻫ ﺪﻨﺷﺎﺑ ﻪﺘﺷاد دﻮﺟو ﻞﺴﻋ رد ﺪﻨﻧاﻮﺗ ﻲﻣ رﻮﭙﺳا ﻞﻜﺷ ﻪﺑ ﺎﻫ )
5 ( .
ﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ مﻮﻨﻴﻟﻮﺗﻮ
رد و ﺖﺳا يزاﻮﻫ ﻲﺑ و ﺖﺒﺜﻣ مﺮﮔ يﺮﺘﻛﺎﺑ
دراد دﻮﺟو ﻲﻌﻴﺒﻃ ترﻮﺻ ﻪﺑ ﺎﺟ ﻪﻤﻫ .
ًﻻﻮﻤﻌﻣ و رﺎﺒﻏ و كﺎﺧ رد
دﻮﺷ ﻲﻣ هﺪﻳد رﻮﭙﺳا ترﻮﺻ ﻪﺑ ﺮﺘﺸﻴﺑ يزروﺎﺸﻛ تﻻﻮﺼﺤﻣ رد .
يﺮﺘﻛﺎﺑ ﻦﻳا ﻢﺳ ﺪﻴﻟﻮﺗ ﺎﺑ ﺪﻧاﻮﺗ ﻲﻣ و هدﻮﺑ رﻮﭙﺳا ﺪﻴﻟﻮﺗ ﻪﺑ ردﺎﻗ
يرﺎﻤﻴﺑ ﺚﻋﺎﺑ هﺪﻨﺸﻛ ﺎﻳ ﻞﺷ ﻲﺠﻠﻓ
دﻮﺷ ﻢﺴﻴﻟﻮﺗﻮﺑ .
ﻲﻳاﺬﻏ ي ﻪﻧﻮﻤﻧ رد يﺮﺘﻛﺎﺑ ﺎﻳ و ﻢﺳ ﻦﺘﻓﺎﻳ ﺎﺑ ﻢﺴﻴﻟﻮﺗﻮﺑ ﺺﻴﺨﺸﺗ ﺖﺳا ﺮﻳﺬﭘ نﺎﻜﻣا كﻮﻜﺸﻣ .
ﻪﭼﺮﮔا ﻪﺑ ترﻮﺻ ﻖﻴﻗد زود ﻲﮔﺪﻨﺸﻛزود
ﻦﻴﺴﻛﻮﺗ مﻮﻨﻴﻟﻮﺗﻮﺑ ﭗﻴﺗ نﺎﺴﻧا ﻚﻳ ياﺮﺑ ارA 70
يﺪﻳرو ﻖﻳرﺰﺗ ترﻮﺻ رد ﻲﻣﺮﮔﻮﻠﻴﻛ 9
/ 0 هار زا و مﺮﮔوﺮﻜﻴﻣ
نﺎﻫد 70 ﺖﺳا هﺪﺷ ﺺﺨﺸﻣ مﺮﮔوﺮﻜﻴﻣ )
6 ( .
ﻢﺳ ﻟﻮﺗﻮﺑ ﻴ مﻮﻨ بﺬﺟ زا ﺲﭘ و هدﻮﺑ موﺎﻘﻣ هﺪﻌﻣ يﺪﻴﺳا ﻂﻳاﺮﺷ ﻪﺑ
ﻲﺒﺼﻋ يﺎﻬﻟﻮﻠﺳ ﻪﺑ نﻮﺧ ﻂﺳﻮﺗ هدور زا ﺪﺳر ﻲﻣ
. مﻮﻤﺳ
مﻮﻨﻴﻟﻮﺗﻮﺑ عاﻮﻧا ﻪﺑ دﻮﺧ ﻲﻜﻴﺘﻧژ ﻲﺘﻧآ توﺎﻔﺗ سﺎﺳا ﺮﺑ A, B, C,
D, E, F, G ﺗ
عاﻮﻧا ﻪﻛ ﺪﻧﻮﺷ ﻲﻣ يﺪﻨﺑ ﻢﻴﺴﻘ B ,E ,F
Aو رد
نﺎﺴﻧا ﺪﻧﻮﺷ ﻲﻣ يرﺎﻤﻴﺑ ﺐﺟﻮﻣ .
عاﻮﻧا ﻪﻛ ﻲﻟﺎﺣ رد وC
ردD
و هﺪﺷ يرﺎﻤﻴﺑ ﺐﺟﻮﻣ تﺎﻧاﻮﻴﺣ زا
G عﻮﻧ ﻲﺻﺎﺧ يرﺎﻤﻴﺑ نﻮﻨﻛ ﺎﺗ
ﺖﺳا هﺪﺸﻧ شراﺰﮔ .
ﻢﺴﻴﻟﻮﺗﻮﺑ دراﻮﻣ زا يرﺎﻴﺴﺑ ﻪﻠﻘﺘﻨﻣ
زا اﺬﻏ
ﻦﻴﺴﻛﻮﺗورﻮﻧ ﻪﺑ هﺪﺷ هدﻮﻟآ ياﺬﻏ فﺮﺼﻣ زا ﻲﺷﺎﻧ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
مﻮﻨﻴﻟﻮﺗﻮﺑ ﺖﺳا ) 8 ، 7 .(
ﻢﺳ ندرﻮﺧ زا ﻲﺷﺎﻧ ،ﻞﺷ ﻲﺠﻠﻓ ﻲﮔﮋﻳو ﺎﺑ ﻲﻳاﺬﻏ ﻢﺴﻴﻟﻮﺗﻮﺑ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ داﻮﻣ ﺎﻳ هﺪﺸﻧ وﺮﺴﻨﻛ بﻮﺧ ﻲﻳاﺬﻏ داﻮﻣ رد
دﻮﺷ ﻲﻣ دﺎﺠﻳا ،ﺪﻧراد يزاﻮﻫ ﻲﺑ ﻂﻳاﺮﺷ ﻪﻛ ﻲﻳاﺬﻏ .
عﻮﻧ رد
رﻮﭙﺳا نﺪﺷ لﺎﻌﻓ ﻪﺠﻴﺘﻧ رد و ﻊﻠﺑ ﺖﻠﻋ ﻪﺑ يدازﻮﻧ ﻢﺴﻴﻟﻮﺗﻮﺑ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ ﺳ ﺪﻴﻟﻮﺗ ﺐﺒﺳ ،نﻮﻟﻮﻛ رد
يرﺎﻤﻴﺑ زوﺮﺑ و ﻢ
دﻮﺷ ﻲﻣ . هﺎﮕﺘﺳد ﻪﺑ يﺮﺘﻛﺎﺑ دورو ﻊﺒﻨﻣ يدازﻮﻧ ﻢﺴﻴﻟﻮﺗﻮﺑ رد
ﻦﻳا زا ﻲﻜﻳ ناﻮﻨﻋ ﻪﺑ ﻞﺴﻋ ﻲﻟو ﺖﺴﻴﻧ ﺺﺨﺸﻣ ﻼﻣﺎﻛ شراﻮﮔ ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﻲﻟﺎﻤﺘﺣا ﻊﺑﺎﻨﻣ )
9 ( .
يﺎﻫرﻮﺸﻛ رد ﻞﺴﻋ فﺮﺼﻣ زا ﻲﺷﺎﻧ ﻢﺴﻴﻟﻮﺗﻮﺑ زوﺮﺑ لﺎﺜﻣ ناﻮﻨﻋ ﻪﺑ ناﺮﻳا ) 10 ( لﺎﻘﺗﺮﭘ ، ) 11 ( ﺎﻴﻟﺎﺘﻳا ، ) 13
، 12 ( ﺮﻓ و ﻪﺴﻧا ) 14 ( شراﺰﮔ
ﺖﺳا هﺪﺷ . رﻮﭙﺳا نﺰﺨﻣ ناﻮﻨﻋ ﻪﺑ ﻞﺴﻋ ﻣﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
ﭗﻴﺘ
وA ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷB )
15 ( . ﻊﻳﺎﺷ زا ﻲﻜﻳ ناﻮﻨﻋ ﻪﺑ ﻞﺴﻋ
ﺎﻜﻳﺮﻣآ لﺎﻤﺷ و ﻦﭘاژ رد نادازﻮﻧ ﻢﺴﻴﻟﻮﺗﻮﺑ زوﺮﺑ ﻞﻠﻋ ﻦﻳﺮﺗ
ﺪﺷﺎﺑ ﻲﻣ ) 16 ( . ﻪﺑ ﺎﭘورا قﺮﺷ يﺎﻫرﻮﺸﻛ رد يرﺎﻤﻴﺑ زوﺮﺑ ﺮﻄﺧ
فﺮﺼﻣ ﻞﻴﻟد ﻂﺳﻮﺗ ﻲﻳوراد نﺎﻫﺎﻴﮔ و يﺎﭼ ﺎﺑ هاﺮﻤﻫ ﻞﺴﻋ
ﺮﺘﺸﻴﺑ نآ رد دﻮﺟﻮﻣ ﻲﻟﺎﻤﺘﺣا يﺎﻫرﻮﭙﺳا ﻪﺑ ﻪﺟﻮﺗ نوﺪﺑ نادازﻮﻧ ﺖﺳا ) 17 ( .
مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا ﻲﻧاواﺮﻓ ناﺰﻴﻣ درﻮﻣ رد ﻲﻧاواﺮﻓ تﺎﻌﻟﺎﻄﻣ نﺎﻬﺟ ﻒﻠﺘﺨﻣ يﺎﻫرﻮﺸﻛ رد هﺪﺷ ﺪﻴﻟﻮﺗ يﺎﻫ ﻞﺴﻋ رد مﻮﻨﻴﻟﻮﺗﻮﺑ ﺖﺳا ﻪﺘﻓﺮﮔ ترﻮﺻ Küplülü .
نارﺎﻜﻤﻫ و 5
/ 12 % يﺎﻫ ﻞﺴﻋ زا
رﻮﭙﺳا ﻪﺑ هدﻮﻟآ ار ﻪﻴﻛﺮﺗ ﺪﻧدﺮﻛ شراﺰﮔ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
) 18 (.
Nevas نﺎﺸﻧ ژوﺮﻧ و ﺪﺋﻮﺳ ،كرﺎﻤﻧاد يﺎﻫرﻮﺸﻛ رد نارﺎﻜﻤﻫ و
ﻪﻛ ﺪﻧداد ،كرﺎﻤﻧاد ﻪﺑ طﻮﺑﺮﻣ ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ رد ﻲﮔدﻮﻟآ عﻮﻴﺷ
ﺪﺋﻮﺳ ﺐﻴﺗﺮﺗ ﻪﺑ ژوﺮﻧ و 26
%
، 10
% و 2
% دﻮﺑ . ﭗﻴﺗوﺮﺳ ﻦﻳﺮﺘﺸﻴﺑ
هﺪﺷ ﻲﻳﺎﺳﺎﻨﺷ هوﺮﮔ ﻪﻌﻟﺎﻄﻣ ﻦﻳارد
دﻮﺑ B ) 19 ( . رد ﻦﻴﻨﭽﻤﻫ
ي ﻪﻌﻟﺎﻄﻣ نارﺎﻜﻤﻫ وRall
،ﻞﻳزﺮﺑ رد 3
% ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ زا
،
رﻮﭙﺳا ﻪﺑ هدﻮﻟآ ﺪﻧدﻮﺑ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
) 20 .( Iurlina و
يﺎﻫ ﻞﺴﻋ ﻲﻜﻳژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ تﺎﻴﺻﻮﺼﺧ ﻲﺳرﺮﺑ رد نارﺎﻜﻤﻫ
يﺎﻫ ﻪﻧﻮﮔ زا ﻚﻳ ﭻﻴﻫ يزﺎﺳاﺪﺟ ﻪﺑ ﻖﻓﻮﻣ ﻦﻴﺘﻧاژرآ ﻒﻠﺘﺨﻣ ﺘﻛﺎﺑ ﺪﻧﺪﺸﻧ ﻞﺴﻋ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ يﺮ )
21 .(
ﺖﻴﻤﻫا زا ﺰﻴﻧ ﻲﭼرﺎﻗ ﻲﮔدﻮﻟآ ،ﻞﺴﻋ يﺎﻫ هﺪﻨﻨﻛ هدﻮﻟآ نﺎﻴﻣ رد ﻦﻳا ياﺮﺑ ناﺮﻳا ﻲﻠﻣ دراﺪﻧﺎﺘﺳا رد و هدﻮﺑ رادرﻮﺧﺮﺑ ﻲﻳﻻﺎﺑ ﺖﺳا هﺪﺷ ﻒﻳﺮﻌﺗ يزﺎﺠﻣ دوﺪﺣ ﻲﮔدﻮﻟآ .
ﻪﻨﻣاد ﻟﺎﻌﻓ ﻴ ﺖ ﺑآ ﻲ
ﻞﺴﻋ ﺑﻴ ﻦ 67 / 0 - 53 / 0 ،ﺪﺷﺎﺑ ﻲﻣ ﻪﻛ زا ﻲﺑآ ﺖﻴﻟﺎﻌﻓ ﻦﻳا ﺪﺷر
ﺮﺜﻛا
ﻣﻴ ﻧﺎﮔراوﺮﻜ ﻴ ﻢﺴ ﺎﻫ ﺪﻨﻛ ﻲﻣ يﺮﻴﮔﻮﻠﺟ .
اﺮﻓ ﻳ ﺪﻨ ﻤﺨﺗ ﻴ ﺮ رد ﻞﺴﻋ
ﻧﺎﻣز ﻲ ترﻮﺻ ﻣ ﻲ ﮔ ﻴ دﺮ ﻪﻛ ﺖﺑﻮﻃر نآ ﻻﺎﺑ ي 171 ﻳﺎ 200 مﺮﮔ
ﻛرد ﻴ مﺮﮔﻮﻠ ﺪﺷﺎﺑ . ﺮﮔا ﺖﺑﻮﻃر ﻞﺴﻋ ﻻﺎﺑ ﺪﺣ ﻦﻳا زا ﺮﺗ ﺪﺷﺎﺑ
ﺎﻫﺮﻤﺨﻣ ي ﻓﻮﻤﺳا ﻴﻠ ﻴ ﻚ ﻦﻜﻤﻣ ﺖﺳا ﺪﻨﻨﻛﺪﺷر . ﺮﺛا اﻳ ﻦ
ﻣﻴ ﻧﺎﮔراوﺮﻜ ﻴ ﻢﺴ ﺎﻫ ﺮﺑ ﺰﻛﻮﻠﮔ و زﻮﺘﻛوﺮﻓ ﺮﺠﻨﻣ ﻪﺑ ﻟﻮﺗ ﻴ ﺪ د ي ﺴﻛا
ﻴ ﻦﺑﺮﻛﺪ و لﻮﻧﺎﺗا ﻣ ﻲ دﻮﺷ و ﻦﻜﻤﻣ ﺖﺳا ﻟﻮﺗ ﻴ ﺪ ﺳا ﻴ ﺪ ﺘﺳا ﻴ ﻚ
ﺎﻤﻧ ﻳ ﺪ ) 22 (.
رﻮﭙﺳا ﻲﻧاواﺮﻓ ﺖﻴﻌﺿو زا ﻲﻓﺎﻛ تﺎﻋﻼﻃا دﻮﺒﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻲﮔدﻮﻟآ ناﺰﻴﻣ و ناﺮﻳا ﺪﻴﻟﻮﺗ يﺎﻫ ﻞﺴﻋ رد مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ مﺎﺠﻧا ﻲﻌﻄﻘﻣ ﻲﻔﻴﺻﻮﺗ ي ﻪﻌﻟﺎﻄﻣ ﻦﻳا ﺮﻤﺨﻣ و ﻚﭙﻛ ﻪﺑ ﺎﻫ نآ ﺖﻓﺮﮔ . ﻲﻧاواﺮﻓ ﻲﺑﺎﻳزرا ،ﻖﻴﻘﺤﺗ ﻦﻳا زا فﺪﻫ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا
لﺎﺳ رد ناﺮﻳا يﺪﻴﻟﻮﺗ يﺎﻫ ﻞﺴﻋ رد ﻲﭼرﺎﻗ ﻲﮔدﻮﻟآ و مﻮﻨﻴﻟﻮﺗﻮﺑ 1392 دﻮﺑ . ﻲﺳرﺮﺑ شور
لﺎﺳ رد ﻪﻛ هدﻮﺑ ﻲﻌﻄﻘﻣ ﻲﻔﻴﺻﻮﺗ عﻮﻧ زا ﻪﻌﻟﺎﻄﻣ ﻦﻳا 1392
ﺮﺑ
داﺪﻌﺗ يور 130
داﺪﻌﺗ ﻦﻳا زا ،ﺖﻓﺮﮔ مﺎﺠﻧا ﻞﺴﻋ ﻪﻧﻮﻤﻧ 126
ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ رﻮﺸﻛ ﻒﻠﺘﺨﻣ يﺎﻫ نﺎﺘﺳا يﺎﻫ نﺎﺘﺳرﻮﺒﻧز زا ﻪﻧﻮﻤﻧ ﻞﺴﻋ ﺪﻴﻟﻮﺗ سﺎﺳا ﺮﺑ ناﺮﻳا ي ﻪﻧﺎﮔ ﺖﻔﻫ يﺪﻨﺑ ﻪﻴﺣﺎﻧ )
لﺎﻤﺷ ،لﺎﻤﺷ
ﺰﻛﺮﻣ و قﺮﺷ لﺎﻤﺷ ،قﺮﺷ ،بﻮﻨﺟ ،بﺮﻏ،بﺮﻏ (
ترﻮﺻ ﻪﺑ
داﺪﻌﺗ و ﺪﺷ ﻪﻴﻬﺗ و بﺎﺨﺘﻧا ﻲﻓدﺎﺼﺗ 4
فوﺮﻌﻣ يﺎﻫ ﺪﻧﺮﺑ زا ﻪﻧﻮﻤﻧ
ﻞﺴﻋ ) ﻲﺘﻌﻨﺻ شور ﻪﺑ ﻞﺴﻋ يﺪﻨﺑ ﻪﺘﺴﺑ و يرواﺮﻓ ﺰﻛاﺮﻣ (
ﻪﻧﻮﻤﻧ
ﺪﺷ يرادﺮﺑ .
داﺪﻌﺗ 126 ﺎﺘﺳرﻮﺒﻧز زا ﻞﺴﻋ ﻪﻧﻮﻤﻧ يﺎﻫ نﺎﺘﺳا ﻒﻠﺘﺨﻣ يﺎﻫ ن
ﺪﻴﻟﻮﺗ سﺎﺳا ﺮﺑ ناﺮﻳا ي ﻪﻧﺎﮔ ﺖﻔﻫ يﺪﻨﺑ ﻪﻴﺣﺎﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ رﻮﺸﻛ ﻞﺴﻋ ) و قﺮﺷ لﺎﻤﺷ ،قﺮﺷ ،بﻮﻨﺟ ،بﺮﻏ ،بﺮﻏ لﺎﻤﺷ ،لﺎﻤﺷ
ﺰﻛﺮﻣ ( ﺪﻧﺪﺷ بﺎﺨﺘﻧا ﻲﻓدﺎﺼﺗ ترﻮﺻ ﻪﺑ .
،داﺪﻌﺗ ﻦﻳا زا 30
ﻪﻧﻮﻤﻧ
،مﻮﻣ ﺎﺑ ﻞﺴﻋ 44
داﺪﻌﺗ و ﻲﻠﮔ ﻚﺗ ﻞﺴﻋ ﻪﻧﻮﻤﻧ 52
ﻞﺴﻋ ﻪﻧﻮﻤﻧ
ﻳدﺮﮔ بﺎﺨﺘﻧا ﻲﻠﮔ ﺪﻨﭼ ﺪ
. ﻞﺴﻋ ﻪﻧﻮﻤﻧ يزﺎﺳ هدﺎﻣآ رﻮﻈﻨﻣ ﻪﺑ
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا نﻮﻣزآ ﺖﻬﺟ اﺪﺘﺑا
25 رد ﻞﺴﻋ مﺮﮔ
100 هﺪﻨﻨﻛ ﻖﻴﻗر ﺎﺑ ﻞﻳﺮﺘﺳا ﺮﻄﻘﻣ بآ ﺮﺘﻴﻟ ﻲﻠﻴﻣ يوﺎﺣ ﺮﮕﻨﻳر ي
ﺪﺻرد ﻚﻳ Tween 80
، ﻞﻣﺎﻛ نﺪﺷ نژﻮﻤﻫ ﺎﺗ و هﺪﺷ ﻪﻓﺎﺿا
تﺪﻣ ﻪﺑ ﻪﺟرد 30
ﻪﺘﺷاﺬﮔ ﻪﻘﻴﻗد و هﺪﺷ
طﻮﻠﺨﻣ ،ﺪﻌﺑ ﻪﻠﺣﺮﻣ رد
رود ﺎﺑ ﻪﻠﺻﺎﺣ 9000
تﺪﻣ ﻪﺑ و ﻪﻘﻴﻗد رد رود 30
ژﻮﻴﻔﻳﺮﺘﻧﺎﺳ ﻪﻘﻴﻗد
ﺪﺷ . ﻪﻠﺻﺎﺣ تﺎﺑﻮﺳر و ﺪﻳدﺮﮔ طﻮﻠﺨﻣ يژﻮﻟﻮﻳﺰﻴﻓ مﺮﺳ ﺮﺘﻴﻟ ﻲﻠﻴﻣ ﻢﻴﻧ ﺎﺑ
ﻪﺑ 9 زا ﺮﺘﻴﻟ ﻲﻠﻴﻣ ﻪﺘﺨﭘ ﺖﺷﻮﮔ ﻂﻴﺤﻣ
)
،كﺮﻣ ،نﺎﻤﻟآ
(CMM
يﺎﻫ ﻪﻟﻮﻟ رد 15
ﺪﺷ ﻪﻓﺎﺿا يﺮﺘﻴﻟ ﻲﻠﻴﻣ .
ﻪﻠﻴﺳو ﻪﺑ ﺲﭙﺳ ﻦﻏور
ﻦﻴﻓارﺎﭘ ﻞﻳﺮﺘﺳا هﺪﻴﺷﻮﭘ ﺖﺸﻛ ﻂﻴﺤﻣ يور هﺪﺷ
ﻲﺑ ﻂﻳاﺮﺷ ﺎﺗ
دﻮﺷ ﻢﻫاﺮﻓ يﺮﺘﻛﺎﺑ ياﺮﺑ يزاﻮﻫ .
ﻂﻴﺤﻣ يﺎﻫ
تﺪﻣ ﻪﺑ ﺖﺸﻛ
10
يﺎﻣد رد زور 35
ﺪﺷ يراﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ ﻪﺟرد ﺪﻧ
.
ﺖﺷﺬﮔ زا ﺪﻌﺑ
زﺎﮔ ﺪﻴﻟﻮﺗ و تروﺪﻛ ﺮﻈﻧ زا ﺖﺸﻛ ﻂﻴﺤﻣ يﺎﻫ ﻪﻟﻮﻟ ،نﺎﻣز ﻦﻳا راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ ﺪﻨﺘﻓﺮﮔ
) 23 (.
ﺲﻧﮋﻧﺮﻓﺮﭘ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ يﺮﺘﻛﺎﺑ زا PTCC:1766
زا هﺪﺷ ﻪﻴﻬﺗ
ناﺮﻳا ﻲﺘﻌﻨﺻ و ﻲﻤﻠﻋ يﺎﻫ ﺶﻫوﮋﭘ نﺎﻣزﺎﺳ ﺖﺒﺜﻣ لﺮﺘﻨﻛ ناﻮﻨﻋ ﻪﺑ
ﺪﺷ هدﺎﻔﺘﺳا .
هﺪﺷ ﻪﺘﺨﭘ ﺖﺷﻮﮔ رد ﺪﺷر زا ﺲﭘ يﺮﺘﻛﺎﺑ
Cooked Meat) (
ﻢﺨﺗ هدرز ﻂﻴﺤﻣ ﻪﺑ Egg Yolk)
( غﺮﻣ
ﺪﻨﻨﻛ ﻖﻴﻗر ﺮﺘﻴﻟ ﻲﻠﻴﻣ ﻚﻳ رد ار ﻪﻠﺻﺎﺣ يﺎﻫ ﻪﻨﮔﺮﭘ و ﺪﺷ ﺢﻴﻘﻠﺗ ه
ﺪﺻرد ﻚﻳ يوﺎﺣ ﺮﮕﻨﻳر Tween 80
كﻮﺷ ﺖﺤﺗ و هدﺮﻛ ﻞﺣ
ﻲﺗراﺮﺣ 65 تﺪﻣ ﻪﺑ ﻪﺟرد 30
ﺪﻧﺪﺷ هداد راﺮﻗ ﻪﻘﻴﻗد .
يﺎﻬﻳﺮﺘﻛﺎﺑ يوﺎﺣ لﻮﻠﺤﻣ زا ﺮﺘﻴﻟ ﻲﻠﻴﻣ ﻚﻳ ﻞﻳﺮﺘﺳا ﻂﻳاﺮﺷ رد ﻪﺑ ار رادرﻮﭙﺳا 25
ﻪﻧﻮﻤﻧ هاﺮﻤﻫو ﺪﻧﺪﺷ ﻪﻓﺎﺿا ﻞﺴﻋ ﻪﻧﻮﻤﻧ مﺮﮔ
ﺖﺸﻛ هﺪﻣآ ﺖﺳد ﻪﺑ تﺎﺑﻮﺳر ژﻮﻴﻔﻳﺮﺘﻧﺎﺳ زا ﺲﭘ ﺶﻳﺎﻣزآ يﺎﻫ
ﺪﻧﺪﺷ هداد .
ﺖﺷﺬﮔ زا ﺲﭘ ﺖﺒﺜﻣ لﺮﺘﻨﻛ ﻪﻧﻮﻤﻧ 10
هدﺮﻛ دﺎﺠﻳا تروﺪﻛ زور
مﺎﺠﻧا رﻮﻈﻨﻣ ﻪﺑ و نﻮﻣزآ
ﺎﺗ ﻴﻳ ﺪ ،ي ﻪﺑ ﺶﻳﺎﻣزآ يﺎﻫ ﻪﻟﻮﻟ 25
هوﺮﮔ
5 ترﻮﺻ ماﺮﮔ يﺰﻴﻣآ ﮓﻧر هوﺮﮔ ﺮﻫ زا و ﺪﻧﺪﺷ ﻢﻴﺴﻘﺗ ﻲﻳﺎﺗ ﺪﻧﺪﺷ يراﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ ﺎﻫ ﻪﻧﻮﻤﻧ ﺮﮕﻳد هاﺮﻤﻫ ندﺮﻛ ﻞﻳﺮﺘﺳا زا
.
ﺖﺷﺬﮔ زا ﺲﭘ ﻲﻔﻨﻣ لﺮﺘﻨﻛ يﺎﻫ ﻪﻧﻮﻤﻧ 10
ﺎﻬﻧآ رد يﺪﺷر زور
ﺖﻓﺮﮕﻧ ترﻮﺻ .
زآرﻮﻈﻨﻣ ﻪﺑ راﺪﻘﻣ ﺮﻤﺨﻣ و ﻚﭙﻛ نﻮﻣ
5 ﺲﭘ ،ﺪﺷ نزو ﻞﺴﻋ مﺮﮔ
يوﺎﺣ ﻞﻳﺮﺘﺳا ﺮﮕﻨﻳر لﻮﻠﺤﻣ ﺎﺑ يزﺎﺳ ﻖﻴﻗر زا 05
/ 0 ﺪﺻرد
Tween 80 ﺖﻗر زا
01 / 0
ﻂﻴﺤﻣ رد ﻲﺤﻄﺳ ﺖﺸﻛ ترﻮﺻ ﻪﺑ
ﺮﻤﺨﻣ هرﺎﺼﻋ -
زوﺮﺘﺴﻛد لﻮﻜﻴﻨﻔﻣاﺮﻠﻛ–
YGC) (
هداد ﺖﺸﻛ
تﺪﻣ ﻪﺑ و ﺪﺷ 3
ﺎﺗ 5 يﺎﻣد رد زور 25
ﺖﻓﺮﮔ راﺮﻗ ﻪﺟرد )
24 ( .
دراﺪﻧﺎﺘﺳا ﻖﺒﻃ هرﺎﻤﺷ ناﺮﻳا ﻲﻠﻣ
92
ﺮﻤﺨﻣ و ﻚﭙﻛ زﺎﺠﻣ ﺪﺣ
زا ﺮﺘﻤﻛ 100 ﺪﺷﺎﺑ ﻲﻣ ﻞﺴﻋ مﺮﮔ ﺮﻫ رد دﺪﻋ )
25 (.
ﻲﻜﻨﻴﺴﻠﻫ ﻪﻧﺎﻴﺑ ﻖﺒﻃ ﻪﻟﺎﻘﻣ ﻦﻳا ﻪﺑ طﻮﺑﺮﻣ ﻲﻗﻼﺧا تﺎﻈﺣﻼﻣ ﻪﻴﻠﻛ ﺖﺳا هﺪﻳدﺮﮔ ﺖﻳﺎﻋر .
ﺎﻫ ﻪﺘﻓﺎﻳ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ يﺮﺘﻛﺎﺑ ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ زا ﻚﻳ ﭻﻴﻫ رد
ﺪﺸﻧ يزﺎﺳاﺪﺟ .
لﺮﺘﻨﻛ هوﺮﮔ رد ﻲﻟو
مﻮﻳﺪﻳﺮﺘﺴﻠﻛ يﺮﺘﻛﺎﺑ
دﻮﻤﻧ تروﺪﻛ دﺎﺠﻳا و هدﺮﻛ ﺪﺷر ﺲﻧﮋﻧﺮﻓﺮﭘ .
زا ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ
ﺮﻈﻧ زا ﻪﻛ داد نﺎﺸﻧ مﻮﺳ زور رد ﺮﻤﺨﻣ و ﻚﭙﻛ شرﺎﻤﺷ و ﺖﺸﻛ ﺐﻴﺗﺮﺗ ﻪﺑ ﺮﻤﺨﻣو ﻚﭙﻛ رﻮﭙﺳا ﻪﺑ ﻲﮔدﻮﻟآ نﺎﺘﺳدﺮﻛ
67 / 66 %
ﮔدﻮﻟآ ﻲ
، هﺎﺸﻧﺎﻣﺮﻛ 56 / 55
%
، ﻢﻗ 50
%
، ﺑدرا ﻴ
،ﻞ ﺰﻛﺮﻣ ي
، ﻳ دﺰ و
ناﺪﻤﻫ /33 33
%
، نﺎﺳاﺮﺧ و ﺒﻋرﺪﻨﺑ سﺎ 25
%
، نﺎﺠﻧز و نﺎﻬﻔﺻا
67 / 16
%
، ﺷ ﻴ زاﺮ 11 / 11
%
، ﺑﻴ ﺮﺘﺸ ﻳ ﻦ ﻣﻴ ناﺰ ﮔدﻮﻟآ ﻲ ﺮﺗﻻﺎﺑ زا ﺪﺣ
زﺎﺠﻣ ار ﻪﺘﺷاد ﺪﻧا . رد ﻟﺎﺣ ﻲ ﻪﻛ ﻪﻧﻮﻤﻧ ﺎﻫ ي
،نﺎﮔﺮﮔ نارﺪﻧزﺎﻣ و
ﺮﺒﺗ ﻳ ﺰ زا ﮔدﻮﻟآﺮﻈﻧ ﻲ ﻪﺑ ﻚﭙﻛ ﺮﻤﺨﻣو رد دوﺪﺣ زﺎﺠﻣ دراﺪﻧﺎﺘﺳا
ﻠﻣ ﻲ اﻳ ناﺮ هدﻮﺑ ﺪﻧا ) لوﺪﺟ 1 .(
لوﺪﺟ :1 ﻲﮔدﻮﻟآ ﻪﻨﻣاد ،رﺎﻴﻌﻣ فاﺮﺤﻧا ،ﻦﻴﮕﻧﺎﻴﻣ (CFU/gr)
ﻪﻧﻮﻤﻧ رد ﺮﻤﺨﻣ و ﻚﭙﻛ ﻪﺑ ﻲﮔدﻮﻟآ زﺎﺠﻣ ﺪﺣ زا ﺮﺗﻻﺎﺑ يﺎﻫ ﻪﻧﻮﻤﻧ ﺪﺻرد و ناﺮﻳا ﻒﻠﺘﺨﻣ ﻖﻃﺎﻨﻣ يﺎﻫ ﻞﺴﻋ
ﻦﻴﮕﻧﺎﻴﻣ
رﺎﻴﻌﻣ فاﺮﺤﻧا± ﻪﻨﻣاد ﺪﺣ زا ﺮﺗﻻﺎﺑ يﺎﻫ ﻪﻧﻮﻤﻧ% دراﺪﻧﺎﺘﺳا زﺎﺠﻣ
ﻪﻧﻮﻤﻧ داﺪﻌﺗ ﻲﻳﺎﻴﻓاﺮﻐﺟ ﻪﻘﻄﻨﻣ
86 /
±27 5 /
36 <30-65 0 10 ﺰﻳﺮﺒﺗ
لﺎﻤﺷ و بﺮﻏ بﺮﻏ
ﺰﻛﺮﻣ 38
/
±27 33 /
35 <30-65 33/33 12 ﻞﻴﺑدرا
33 /
±178 43 /
104 70-340 66/67 9 نﺎﺘﺳدﺮﻛ
33 /
±138 15 /
63 65-240 55/56 9 هﺎﺸﻧﺎﻣﺮﻛ
±70 24 /
68 45-120 16/67 9 نﺎﺠﻧز
33 /
±90 26 /
41 43-162 33/33 9 ناﺪﻤﻫ
43 /
±19 5 / 66
08 /
±104 67 / 150
65 -
<30 300 - 35
11 / 11
50
9 9
زاﺮﻴﺷ ﻢﻗ 18
/
±24 67 /
75 39-110 33/33 9 دﺰﻳ
83 /
±68 79 //
24 40-110 16/67 9 نﺎﻬﻔﺻا
5 /
±77 75 /
22 42-110 33/33 9 يﺰﻛﺮﻣ
82 /
±23 83 /
15 <30-65 0 9 نارﺪﻧزﺎﻣ لﺎﻤﺷ
83 /
±27 28 /
29 <30-65 0 9 نﺎﮔﺮﮔ قﺮﺷ لﺎﻤﺷ
98 /
±33 91 /
83 42-162 25 8 سﺎﺒﻋ رﺪﻨﺑ بﻮﻨﺟ
27 /
±26 68 /
75 38-120 25 16 نﺎﺳاﺮﺧ قﺮﺷ
8 /
±45 4 /
79 <30-300 %26/67(34) 130 ﻊﻤﺟ
يﺮﻴﮔ ﻪﺠﻴﺘﻧ و ﺚﺤﺑ
رد 34 ﻪﻧﻮﻤﻧ ) 67 / 26 (%
ﺪﺷ هﺪﻫﺎﺸﻣ ﺮﻤﺨﻣ و ﻚﭙﻛ ﻪﺑ ﻲﮔدﻮﻟآ .
ﺎﺑ نﺎﺘﺳدﺮﻛ نﺎﺘﺳا 6
/ 66 % ﻦﻳﺮﺘﺸﻴﺑ ،زﺎﺠﻣ ﺪﺣ زا ﺮﺗﻻﺎﺑ ﻲﮔدﻮﻟآ
ﻲﮔدﻮﻟآ ﻪﻨﻣاد ﺎﺑ نارﺪﻧزﺎﻣ و ﻲﮔدﻮﻟآ CFU/g
55 - 30 ﻦﻳﺮﺘﻤﻛ
ﺪﻧدﻮﺑ اراد ﺮﻤﺨﻣ و ﻚﭙﻛ ﻪﺑ ﻲﮔدﻮﻟآ ناﺰﻴﻣ .
Kuplulu يا ﻪﻌﻟﺎﻄﻣ ،نارﺎﻜﻤﻫ و
) 2006 ( ﻲﺳرﺮﺑ رﻮﻈﻨﻣ ﻪﺑ
رﻮﭙﺳا رﻮﻀﺣ
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﺸﻛ ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ رد
ﺪﻧداد مﺎﺠﻧا ﻪﻴﻛﺮﺗ ﺪﻧدﻮﻤﻧ ﺺﺨﺸﻣ و
ﻪﻛ 5 / 12 % يﺎﻫ ﻞﺴﻋ زا
ﻪﺑ هدﻮﻟآ ارﺎﻜﻧآ و ﻪﻴﻛﺮﺗ رد ﻲﺷوﺮﻓ هدﺮﺧ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا
مﻮﻨﻴﻟﻮﺗﻮﺑ دﻮﺑ ﺖﺳا ه ) 18 Nevas (.
لﺎﺳ رد نارﺎﻜﻤﻫ و 2005
ﻪﺑ ﻲﻘﻴﻘﺤﺗ ﻲﻃ يﺎﻫ ﭗﻴﺗ عﻮﻴﺷ ﻲﺳرﺮﺑ
,A E ,B F و
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
يﺎﻫرﻮﺸﻛ رد هﺪﺷ ﺪﻴﻟﻮﺗ يﺎﻫ ﻞﺴﻋرد
شور ﻪﺑ ﺎﭘورا ﻲﻟﺎﻤﺷ ﺪﻨﺘﺧادﺮﭘPCR
.
،كرﺎﻤﻧاد ﻪﺑ طﻮﺑﺮﻣ ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ رد ﻲﮔدﻮﻟآ عﻮﻴﺷ
ﺪﺋﻮﺳ و
ﺐﻴﺗﺮﺗ ﻪﺑ ژوﺮﻧ 26
% ، 10
% و 2
% دﻮﺑ . ﻦﻳارد ﻪﻛ ﻲﭙﻴﺗوﺮﺳ ﻦﻳﺮﺘﺸﻴﺑ
ﺳﺎﻨﺷ ﻪﻌﻟﺎﻄﻣ هوﺮﮔ ﺪﺷ ﻲﻳﺎ
B دﻮﺑ ) 19 (.
ﻂﺳﻮﺗ ﻲﻬﺑﺎﺸﻣ ﻪﻌﻟﺎﻄﻣ رد Pablo Schocken
نارﺎﻜﻤﻫ و
) 1999 ( ﻪﺑ ﻞﻳزﺮﺑ يﺎﻫ ﻞﺴﻋ ﻲﮔدﻮﻟآ ناﺰﻴﻣ رﻮﭙﺳا
مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
دﻮﺑ D ) 24 (.
سﺎﺳاﺮﺑ ﻂﺳﻮﺗ ﻪﺘﻓﺮﮔ مﺎﺠﻧا ﺶﻫوﮋﭘ و Rall
نارﺎﻜﻤﻫ ) 2003 (
،ﻞﻳزﺮﺑ رد 3
% ﻪﺑ هدﻮﻟآ ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ زا
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا ﺪﻧدﻮﺑ
. رد 64 % رﻮﭙﺳا ﺎﻫ ﻪﻧﻮﻤﻧ
ﺮﻤﺨﻣ و ﻚﭙﻛ ﺰﻴﻧ
ﺪﺷ ﻲﻳﺎﺳﺎﻨﺷ )
20 ( .
يا ﻪﻌﻟﺎﻄﻣ رد Nevas
نارﺎﻜﻤﻫ و )
2002 ( ﻲﺳرﺮﺑ ﻪﺑ نﺎﻤﻟآ رد
رﻮﭙﺳا رﻮﻀﺣ ﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ
ﻣﻮﻨ يزﺎﺳ ﻲﻨﻏ شور ﻪﺑ ﻞﺴﻋ رﺪ
ﻲﺤﻄﺳ ﻊﻳﺎﻣ داﻮﻣ نﻮﻴﺳاﺮﺘﻠﻴﻓ ﻖﻳﺮﻃ زا SF)
( ﺲﭙﺳ و PCR
ﺪﻨﺘﺧادﺮﭘ . ﺮﻫ رد يﺮﺘﻛﺎﺑ يﺎﻫ رﻮﭙﺳا داﺪﻌﺗ ﻪﻨﻣاد ﻲﻤﻛ ﺮﻈﻧ زا
ﻞﺴﻋ مﺮﮔﻮﻠﻴﻛ 140
- 18 دﻮﺑ دﺪﻋ .
A ﭗﻴﺗ رد 17 ﭗﻴﺗ ،ﻪﻧﻮﻤﻧ ردB
12 ﺪﺷ ﻲﻳﺎﺳﺎﻨﺷ ﻪﻧﻮﻤﻧ .
رد 9
ﭗﻴﺗ ﺰﻴﻧ ﻪﻧﻮﻤﻧ وA
يﺮﺘﻛﺎﺑB ﻮﺟو ماﻮﺗ رﻮﻃ ﻪﺑ ﺖﺷاد د
) 26 (
Iurlina نارﺎﻜﻤﻫ و )
2005 ( رد
ﻲﺑوﺮﻜﻴﻣ يﺎﻫ ﻪﺼﺨﺸﻣ ﻲﺳرﺮﺑ
ﻦﻴﺘﻧاژرآ ﻒﻠﺘﺨﻣ يﺎﻫ ﻞﺴﻋ ﻪﻧﻮﻤﻧ يﺎﻫ يﺮﺘﻛﺎﺑ ﻪﻛ ﺪﻧداد نﺎﺸﻧ ،
دراﺪﻧ دﻮﺟو ﺎﻫ ﻪﻧﻮﻤﻧ رد ﺖﻴﻔﻟﻮﺳ هﺪﻨﻨﻛ ﺎﻴﺣا مﻮﻳﺪﻳﺮﺘﺴﻠﻛ )
21 ( .
Vidon نارﺎﻜﻤﻫ و 1994)
مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا ﻲﻧاواﺮﻓ (
رد ار مﻮﻨﻴﻟﻮﺗﻮﺑ 90
درﻮﻣ ﻪﺴﻧاﺮﻓ قﺮﺷ ﻪﻘﻄﻨﻣ يﺎﻫ ﻞﺴﻋ زا ﻪﻧﻮﻤﻧ
داﺪﻌﺗ نﺎﻴﻣ ﻦﻳا زا ﻪﻛ ﺪﻧداد راﺮﻗ ﻲﺳرﺮﺑ 8
رﻮﭙﺳا ﻪﺑ هدﻮﻟآ ﻪﻧﻮﻤﻧ
دﻮﺑ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ )
9 .( رﻮﭙﺳا رﻮﻀﺣ مﺪﻋ ﻢﻏﺮﻴﻠﻋ اﺬﻟ
ﺮﻄﺧزا هﺪﺷ ﻲﺳرﺮﺑ ﻞﺴﻋ يﺎﻫ ﻪﻧﻮﻤﻧ رد مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ ﻣ ﺮﺜﻛا اﺮﻳز دﻮﺷ ﻲﻤﻧ ﻪﺘﺳﺎﻛ لﺎﺳ ود ﺮﻳز نادازﻮﻧ رد ﻞﺴﻋ فﺮﺼ
ﺪﻨﺷﺎﺑ ﻲﻣ ﻲﻌﻄﻘﻣ ترﻮﺼﺑ هﺪﺷ مﺎﺠﻧا تﺎﻌﻟﺎﻄﻣ .
ﻲﮔدﻮﻟآ ﻦﻳا ﺮﺑﺎﻨﺑ
ﻪﻛ ﺖﺳا ﻲﻄﻴﺤﻣ ﻂﻳاﺮﺷ زا ﺮﺛﺎﺘﻣ مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ رﻮﭙﺳا ﻪﺑ ﺪﺷﺎﺑ توﺎﻔﺘﻣ ﺪﻨﻧاﻮﺗ ﻲﻣ ﻂﻳاﺮﺷ ﻦﻳا نﺎﻣز زا ﺮﮕﻳد يا هزﺎﺑ رد .
زا
ﺎﻘﻣ ﻪﻌﺷا ﻪﺑ ﺖﺒﺴﻧ ﻞﺴﻋ ﻪﻛ ﺎﺠﻧآ ﻦﻳا ﻊﻓر ياﺮﺑ ناﻮﺗ ﻲﻣ ﺖﺳا مو
ﺎﺗ دﺮﻛ ﻲﻧﺎﻣردﻮﺗﺮﭘ ﺎﻣﺎﮔ ﻪﻌﺷا ﺎﺑ ار ﻞﺴﻋ ،هدﺎﻔﺘﺳا زا ﻞﺒﻗ ﻞﻜﺸﻣ دراد ﻲﻳاﻮﻫ و بآ ﻂﻳاﺮﺷ ﺎﺑ ﻲﻤﻴﻘﺘﺴﻣ ﻪﻄﺑار ﺎﻫ ﺮﻤﺨﻣ و ﻚﭙﻛ
.
رد ﻲﮔدﻮﻟآ ،ﺖﺳا ﺺﺨﺸﻣ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺞﻳﺎﺘﻧ زا ﻪﻛ رﻮﻄﻧﺎﻤﻫ ﻣ ﻖﻃﺎﻨﻣ ﺪﺷﺎﺑ ﻲﻣ دﻮﺧ ﺢﻄﺳ ﻦﻳﺮﺘﻤﻛ رد ،رﻮﺸﻛ بﻮﻃﺮ .
ﻚﭙﻛدﻮﺟو و
ردﺮﻤﺨﻣ ﻞﺴﻋ بﺎﻨﺘﺟا ﺎﻧ
ﺬﭘ ﻳ ﺮ ﺖﺳا
، اﺮﭼ ﻪﻛ
رﻮﺒﻧز مﺎﮕﻨﻫ ﻊﻤﺟ روآ ي ﺪﻬﺷ ﺎﻬﻧآ ار ﺎﺑ دﻮﺧ وﺪﻨﻛ ﻪﺑ ﻣ ﻲ دروآ .
ﻲﻣ ﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﻦﻳا ﺪﺷر لﺮﺘﻨﻛ ﺚﻋﺎﺑ نﻮﻴﺳاﺰﻳرﻮﺘﺳﺎﭘ دﻮﺷ . ،ﻞﺴﻋ ﮓﻧر ﺮﻴﻴﻐﺗ ﺚﻋﺎﺑ تراﺮﺣ ﻲﻔﻨﻣ تاﺮﺛا زا ﻲﻀﻌﺑ
ﺎﻫ ﻢﻳﺰﻧآ ﺮﻳﺎﺳ و زﺎﺗرﻮﻨﻳا ﻢﻳﺰﻧآ ﺶﻫﺎﻛو دﻮﺷ ﻲﻣ
) 22 (.
يﺎﻫ ﻞﺴﻋ ندﻮﺑ ﻦﻤﻳا زا ﻲﻛﺎﺣ ﺮﺿﺎﺣ ﺶﻫوﮋﭘ زا ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ زا نﺎﻣز زا ﻲﻌﻄﻘﻣ رد ناﺮﻳا ﻒﻠﺘﺨﻣ ﻖﻃﺎﻨﻣ رد هﺪﺷ ﺪﻴﻟﻮﺗ ﺮﻈﻧ
مﻮﻨﻴﻟﻮﺗﻮﺑ مﻮﻳﺪﻳﺮﺘﺴﻠﻛ ﻣ
ﺪﺷﺎﺑ ﻲ . ﻪﺑ ،ﻲﻄﻴﺤﻣ ﻂﻳاﺮﺷ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
رﻮﻀﺣ نﺎﻣز زا ﺮﮕﻳد يا هزﺎﺑ رد ﺖﺳا ﻦﻜﻤﻣ ﻲﻧﺎﻣز ﻂﻳاﺮﺷ هﮋﻳو ﻢﺴﻴﻟﻮﺗﻮﺑ زوﺮﺑ ﺮﻄﺧ ﺶﻫﺎﻛ رﻮﻈﻨﻣ ﻪﺑ اﺬﻟ ،ﺪﻨﻛ اﺪﻴﭘ ﺶﻳاﺰﻓا رﻮﭙﺳا ﺮﻳز نﺎﻛدﻮﻛ ﻪﺑ نﺎﻜﻣﻻا ﻲﺘﺣ ﻪﻛ دﻮﺷ ﻲﻣ ﻪﻴﺻﻮﺗ نادازﻮﻧ رد 2
دﻮﺸﻧ هﺪﻧارﻮﺧ ﻞﺴﻋ لﺎﺳ .
ﺟ رد
ﻦﻳا رد ﻞﺴﻋ فﺮﺼﻣ ﻪﻛ ﻲﻌﻣاﻮ
زا يﺮﻴﮔﻮﻠﺟ ياﺮﺑ دﻮﺷ ﻲﻣ دﺎﻬﻨﺸﻴﭘ ﺖﺳا هﺪﺷ ﺞﻳار ﻲﻨﺳ هوﺮﮔ ﺎﻣﺎﮔ ي ﻪﻌﺷا ﺎﺑ هدﺎﻔﺘﺳا زا ﻞﺒﻗ ار ﻲﻓﺮﺼﻣ ي ﺎﻫ ﻞﺴﻋ يرﺎﻤﻴﺑ زوﺮﺑ ﺪﻨﻳﺎﻤﻧ ﻲﻫدﻮﺗﺮﭘ .
ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ
ﻲﻀﺗﺮﻣ يﺎﻗآ ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ ﻪﻣﺎﻧ نﺎﻳﺎﭘ زا ﻞﺻﺎﺣ ﻪﻟﺎﻘﻣ ﻦﻳا ﺸﻧاد ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد رد هدازداﻮﺟ
ﺪﻴﻬﺷ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕ
يرﺎﻜﻤﻫ ﺎﺑ و كﺮﺘﺸﻣ حﺮﻃ ترﻮﺻ ﻪﺑ ﻪﻛ هدﻮﺑ دﺰﻳ ﻲﻗوﺪﺻ مﺎﺠﻧا ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﺘﻨﺳ ﺐﻃ تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ ﺖﺳا هﺪﺷ .
ﻦﻳا مﺎﺠﻧا رد ار ﺎﻣ ﻪﻛ ﻲﻧﺎﺴﻛ ﻲﻣﺎﻤﺗ زا ﻪﻠﻴﺳﻮﻨﻳﺪﺑ
ﻢﻴﻳﺎﻤﻧ ﻲﻣ ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ ﺪﻧا هدﻮﻤﻧ يرﺎﻳ ﺶﻫوﮋﭘ .
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Received:2014/4/22 Accepted:2014/5/26
Clostridium Botulinum Spores and Fungal Contamination in Honeys of Iran
Bahador Hajimohammadi (Ph.D)
1, Ali Dehghani (Ph.D)
2, Morteza Javadzadeh (M.Sc)
3, Nabi Shariatifar (Ph.D)
4Hengameh Zandi (Ph.D)
5,
Hassan Mozaffari-khosravi (Ph.D)
6, Gilda Eslami (Ph.D)
71. Assistant Professor Department Food Hygiene and Safety, Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2.Assistant Professor, Department of Epidemiology School of Public Health, Shahid SadoughiUniversity of Medical Sciences, Yazd, Iran
3.Corresponding Author: M.Sc student in Food Hygiene and Safety,School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4. Assistant Professor, Department of Environmental Health Engineering, Traditional medicine research center, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5.Asistant Professor, Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
6.Professor, Department of Nutrition, School of Public Health,ShahidSadoughiUniversity of Medical Sciences, Yazd, Iran
7.Assistant Professor, Department of Parasitologyand Mycology, School of Medicine, Shahid SadoughiUniversity of Medical Sciences, Yazd, Iran
Abstract
Introduction: Microorganisms may be present in the form of spores, in honey. One of these dangerous organisms is the bacterium Clostridium botulinium spores which are capable induced botulism particularly in infants. The aim of this study was to determine the prevalence of Cl. botulinum spores in honey produced in different regions of Iran.
Method: This cross-sectional study was performed in 2013. A total of 130 honey samples were collected and analyzed for Cl. botulinium spore. Samples were chosen randomly from the apiary of Iran. The samples were examined according to Iranian standard. In laboratory, honey samples were investigated using thermal shock and they located in cooked meat medium and incubated for 10 days at 35°C. The bacterium spores of Clostridium perfringens (PTCC: 1766) was used as a positive control sample.
Results:The Results showed that Cl. botulinum spore was not detected in any of examined samples. Thirty four samples (26.67%) had mold and yeast contamination higher than the Iranian standard limit. Samples from Kurdestan province had high contamination (66.6%) and it was higher than other regions. Honey samples from Mazanderan province hadthe lowest prevalenceofmold and yeast with ranged from 30 to 55 CFU/g.
Conclusion: Overall, the contamination of honey with Cl. botulinumspores in examined samples was zero and so they are safe, but some samples had mold and yeasts contamination that honey samples collected from west and north regions had the high and low contamination, respectively.
Keywords: Honey, Cl. botulinum, Mold, Yeast, Food safety
This Paper Should be Cited as:
Bahador Hajimohammadi (Ph.D),Ali Dehghani (Ph.D),Morteza Javadzadeh (M.Sc), Nabi Shariatifar (Ph.D), Hengameh Zandi (Ph.D), Hassan Mozaffari-khosravi (Ph.D), Gilda Eslami (Ph.D).Clostridium Botulinum Spores and Fungal Contamination in Honeys of Iran. Journal Tolooebehdasht Sci
