ﯽﺳرﺮﺑرد ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺮﯿﺛﺎﺗ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺰﺒﺳ ﺎﯿﺑﻮﻟ و هﺎﯿﺳ بﺮﺗ ﯽﻨﺴﺤﻣ ﻢﯾﺮﻣ ﻦﯿﻧزﺎﻧ

(1)

ﯽﺳرﺮﺑ رد ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺮﯿﺛﺎﺗ

رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ

ﺰﺒﺳ ﺎﯿﺑﻮﻟ و هﺎﯿﺳ بﺮﺗ

ﯽﻨﺴﺤﻣ ﻢﯾﺮﻣ ﻦﯿﻧزﺎﻧ

1

ﺮﯿﻣا ،



ﯽﻧﺎﺧ ﻪﻣﺮﮔ ﯽﺋاراد ﯽﻧﺎﺛ يﺪﻤﺤﻣ ﯽﻠﻋ ،

2

3

1 - ناﺮﯾا ،يرﺎﺳ ،ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،يرﺎﺳ ﺪﺣاو ،نﺎﮕﺒﺨﻧ و ناﻮﺟ ناﺮﮕﺸﻫوﮋﭘ هﺎﮕﺷﺎﺑ ﻮﻀﻋ .

2 - ﻠﻋ ﺖﺌﯿﻫ ﻮﻀﻋ ناﺮﯾا ،ناﺪﻤﻫ ،ﺎﻨﯿﺳ ﯽﻠﻋﻮﺑ هﺎﮕﺸﻧاد ،نﺎﮐﺮﺴﯾﻮﺗ ﯽﻨﻓ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ﯽﻤ

.

3 -

،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ﯽﻤﻠﻋ تﺎﯿﻫ ﻮﻀﻋ

،نﺎﭼﻮﻗ ﺪﺣاو ناﺮﯾا ،نﺎﭼﻮﻗ ،ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد

.

) ﺖﻓﺎﯾرد ﺦﯾرﺎﺗ :

30 / 06 / 96 شﺮﯾﺬﭘ ﺦﯾرﺎﺗ :

15 / 11 / 96 (

هﺪﯿﮑﭼ

يﺎﻬﺘﻓﺎﺑ رد دﻮﺟﻮﻣ يﺎﻫ ﻢﯾﺰﻧآ ﺪﻨﺷﺎﺑ تﺎﺠﯾﺰﯿﺳ و ﺎﻫ هﻮﯿﻣ ﺖﯿﻔﯿﮐ يور ﺮﺑ يﺮﻀﻣ و ﺪﯿﻔﻣ تاﺮﺛا ياراد ﺪﻨﻧاﻮﺘﯿﻣ ﯽﻫﺎﯿﮔ

. ﺎﻬﻧآ تاﺮﯿﯿﻐﺗ ﺖﯾﺮﯾﺪﻣ ﻦﯾاﺮﺑﺎﻨﺑ

ﺖﺳا حﺮﻄﻣ ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ رد ﻪﺟﻮﺗ درﻮﻣ ﻞﺋﺎﺴﻣ زا ﯽﮑﯾ ناﻮﻨﻋ ﻪﺑ تﺎﺠﯾﺰﺒﺳ و ﺎﻫ هﻮﯿﻣرد .

هزوﺮﻣا يﺎﺿﺎﻘﺗ زا هدﺎﻔﺘﺳا ﻪﺑ نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ داﻮﻣ

هزﺎﺗ ﯽﯾاﺬﻏ

ﺎﯾ و روآﺮﻓ ﺮﺘﻤﮐ ي ﺖﺳا ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا هﺪﺷ .

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ رد ﻦﺸﯾوآ ﺲﻧﺎﺳا ﯽﯾﺎﻧاﻮﺗ ﯽﺑﺎﯾزرا ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ زا فﺪﻫ )

زا ﯽﮑﯾ ناﻮﻨﻋ ﻪﺑ

دﺎﺴﻓ ﻢﻬﻣ ﻞﻣاﻮﻋ ﻮﯿﺑ

ﯽﯾﺎﯿﻤﯿﺷ ( بﺮﺗ رد هﺎﯿﺳ ﺰﺒﺳ ﺎﯿﺑﻮﻟ و ﺖﺳا

. هرﺎﺼﻋ رﻮﻈﻨﻣ ﻦﯾﺪﺑ ﯽﻫﺎﯿﮔ

يوﺎﺣ ﻢﯾﺰﻧآ ﺎﺑ زاﺪﯿﺴﮐاﺮﭘ طﻮﻠﺨﻣ

،لﻮﮐﺎﯾﺎﮔ ﻞﻣﺎﺷ اﺮﺘﺴﺑﻮﺳ

ﺪﯿﺴﮐاﺮﭘ ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ و ﻢﯾﺪﺳ تﺎﻔﺴﻓ ﺮﻓﺎﺑ ،نژورﺪﯿﻫ ﻦﺸﯾوآ

) 50 ، 75 ، 100 و 200 (g/100 ml ﺪﯾدﺮﮔ طﻮﻠﺨﻣ

. ﺲﭙﺳ ﻪﻧﻮﻤﻧ بﺬﺟ

ﺎﻫ يﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا شور زا هدﺎﻔﺘﺳا ﺎﺑ جﻮﻣ لﻮﻃ رد

470 ﺮﺘﻣﻮﻧﺎﻧ ﻪﺑ تﺪﻣ 400 ﺪﺷ ﺖﺋاﺮﻗ ﻪﯿﻧﺎﺛ .

ﻦﯾا زا ﻞﺻﺎﺣ ﺞﯾﺎﺘﻧ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﮐ داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ

اﺪﯿﺴﮐاﺮﭘ بﺮﺗ رد ز هﺎﯿﺳ 4625 ﺰﺒﺳ ﺎﯿﺑﻮﻟ رد و 51

) يﺰﺒﺳ مﺮﮔ × ﻪﯿﻧﺎﺛ / ﺖﯿﻟﺎﻌﻓ ﺪﺣاو (

ﻪﮐ هدﻮﺑ ندوﺰﻓا ﺎﺑ ﺖﯿﻟﺎﻌﻓ قﻮﻓ يﺎﻫ ﺖﻈﻠﻏ رد ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻢﯾﺰﻧآ

زاﺪﯿﺴﮐاﺮﭘ هﺎﯿﺳ بﺮﺗ ﺐﯿﺗﺮﺗ ﻪﺑ 2655 ، 2550 ، 2015 و 2162 ) يﺰﺒﺳ مﺮﮔ × ﻪﯿﻧﺎﺛ / ﺖﯿﻟﺎﻌﻓ ﺪﺣاو (

ﺖﯿﻟﺎﻌﻓ و ﻢﯾﺰﻧآ زاﺪﯿﺴﮐاﺮﭘ ﺐﯿﺗﺮﺗ ﻪﺑ ﺰﺒﺳ ﺎﯿﺑﻮﻟ 708

،

6/

551 ، 579 و 690 ) يﺰﺒﺳ مﺮﮔ × ﻪﯿﻧﺎﺛ / ﺖﯿﻟﺎﻌﻓ ﺪﺣاو (

ﺖﺳا هﺪﺷ .

ژاو ﺪﯿﻠﮐ نﺎﮔ : زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ،ﻦﺸﯾوآ ﺲﻧﺎﺳا ،ﺰﺒﺳ ﺎﯿﺑﻮﻟ ،هﺎﯿﺳ بﺮﺗ .

تﺎﺒﺗﺎﮑﻣ لﻮﺌﺴﻣ

:

[email protected]

(2)

1 - ﻪﻣﺪﻘﻣ

ﻪﻫد رد ﺖﯿﻌﻤﺟ نوﺰﻓازور ﺪﺷر

ﯽﺑ هدﺎﻔﺘﺳا و ﺮﯿﺧا يﺎﻫ ﻪـﯾور

ﯽﺒﯾﺮﺨﺗ ﺮﯿﺳ ﺶﯾاﺰﻓا ﺚﻋﺎﺑ ﯽﻌﯿﺒﻃ ﻊﺑﺎﻨﻣ زا دوﺪـﺤﻣ ﻊﺑﺎـﻨﻣ ﻦـﯾا

ﺖﺳا هﺪﺷ . ،ﻂﯿﺤﻣ ﺮﺘﺸﯿﺑ ﺐﯾﺮﺨﺗ نوﺪﺑ ﺖﯿﻌﻤﺟ ﻦﯾا ﻢﻟﺎﺳ ﻪﯾﺬﻐﺗ

و ﻞـﺒﻗ تﺎﻌﯾﺎﺿ زا يﺮﯿﮔﻮﻠﺟ ،ﯽﯾاﺬﻏ داﻮﻣ ﺪﯿﻟﻮﺗ ﺶﯾاﺰﻓا ﺪﻨﻣزﺎﯿﻧ و ﻆـﻔﺣ ﺖـﻬﺟ ﯽﯾﺎﯿﻤﯿـﺷ داﻮﻣ دﺮﺑرﺎﮐ ﺶﻫﺎﮐ ،ﺖﺷادﺮﺑ زا ﺲﭘ هدروآﺮـﻓ ﻊـﻗﻮﻣ ﻪـﺑ و ﺎـﺠﺑ فﺮﺼـﻣ مﺎﺠﻧاﺮـﺳ و يراﺪﻬﮕﻧ يﺎـﻫ

ﺖﺳا يزروﺎﺸﮐ .

ﻣ ﺮﺘﺸﯿﺑ ﯽﻠﮐ رﻮﻃ ﻪﺑ هﻮﯿ

ﺖﻠﻋ ﻪﺑ تﺎﺠﯾﺰﺒﺳ و ﺎﻫ

ﺪــﯾﺎﺑ ﺖــﺷادﺮﺑ زا ﺲــﭘ و ﺪﻨﺘﺴــﻫ ﺮﯾﺬﭘدﺎﺴــﻓ دﺎــﯾز بآ ﻦﺘــﺷاد شور ﻪﺑ ﺎﯾ و فﺮﺼﻣ ﻪﻠﺻﺎﻓﻼﺑ ﺪﻧﻮﺷ يراﺪﻬﮕﻧ ﯽﺻﺎﺧ يﺎﻫ

] 1 .[

ﻢﻬﻣ ﻞﺋﺎﺴﻣ زا ﯽﮑﯾ ﯽﻋارز و ﯽﻏﺎﺑ تﻻﻮﺼﺤﻣ رد تﺎﻌﯾﺎﺿ زوﺮﺑ چرﺎﻗ ﻪﻠﻤﺣ زا ﯽﺷﺎﻧ ﺮﻣا ﻦﯾا ﻪﮐ ﺖﺳا يزروﺎﺸﮐ

تﺎـﻓآ و ﺎﻫ ﺎـﯾ

ﻒﻠﺘﺨﻣ يﺎﻫ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﻤﻫ و هدﻮﺑ

ار يدﺎـﯾز ترﺎﺴﺧ ﻪﻟﺎﺳ

ﯽﻣ رﻮﺸﮐ ﻪﺟﻮﺘﻣ ﺪﯾﺎﻤﻧ

. ﻦﺘـﻓر ﺖـﺳد زا ﺮـﺑ هوﻼـﻋ عﻮﺿﻮﻣ ﻦﯾا

،ﯽﭼرﺎـﻗ مﻮﻤﺳ ﺪﯿﻟﻮﺗ ﺖﻠﻋ ﻪﺑ ،يزروﺎﺸﮐ تﻻﻮﺼﺤﻣ زا ﯽﺸﺨﺑ ﺶﮐ چرﺎﻗ فﺮﺼﻣ

ﺖﻓآ و ﺎﻫ ﺶﮐ ﻦﯿـﺑ زا ﺖـﻬﺟ ﯽﯾﺎﯿﻤﯿـﺷ يﺎـﻫ

ﻦﯾا ندﺮﺑ ﺰـﯿﻧ ﻪـﻌﻣﺎﺟ ﺖﻣﻼـﺳ ندﺎﺘﻓا ﺮﻄﺧ ﻪﺑ ﺐﺒﺳ تﺎﻓآ ﻪﻧﻮﮔ

ﯽﻣ ددﺮﮔ . ﺳ رﺎﺛآ هزوﺮﻣا ﺶﮐ چرﺎﻗ فﺮﺼﻣءﻮ

ﯽﯾﺎﯿﻤﯿﺷ مﻮﻤﺳ و ﺎﻫ

ﻪـﺑ ﺎـﯿﻧد يﺎﻫرﻮﺸﮐ قﺎﻔﺗا ﻪﺑ ﺐﯾﺮﻗ ﺮﺜﮐا و هﺪﺷ ﺖﺑﺎﺛ ﻪﻤﻫ ﺮﺑ ﺰﯿﻧ هﺪﻧﺎﻤﯿﻗﺎﺑ زا يرﺎﻋ تﻻﻮﺼﺤﻣ ﺎﯾ ﮏﯿﻧﺎﮔرا تﻻﻮﺼﺤﻣ ﺪﯿﻟﻮﺗ

يﺎﻫ

هﺮﺸﺣ ،مﻮﻤﺳ ﺪﻨﻧﺎﻣ ﺮﻀﻣ هﺪﻨﯾﻻآ

ﺶﮐ ﺶﮐ چرﺎﻗ ،ﺎﻫ و ﺮﻀـﻣ يﺎﻫ

فﺮﺼــﻣ ياﺮــﺑ ﻪــﮐ ﯽﮔدﻮــﻟآ عﻮــﻧ ﺮــﻫ و ﯽﯾﺎﯿﻤﯿــﺷ و نﺎﮔﺪــﻨﻨﮐ

ﻂﯿﺤﻣ ﺖﺴﯾز نﺎﯾز هدروآ يور ﺪﻨﺷﺎﺑ روآ

ﺪﻧا ] 2 .[

ﻪـﺘﮑﻧ ﻦـﯾا ﺮﮐذ

ﺪـﯾﺮﺧ ياﺮـﺑ زرا يدﺎـﯾز ﺮﯾدﺎـﻘﻣ ﻪﻧﻻﺎـﺳ ﻪﮐ ﺖﺳا يروﺮﺿ ﺰﯿﻧ ﺶﮐ چرﺎﻗ و مﻮﻤﺳ

ﯽﻣ جرﺎﺧ رﻮﺸﮐ زا ﯽﯾﺎﯿﻤﯿﺷ يﺎﻫ ددﺮـﮔ

. ﻪـﺑ

ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ نﺎﻣز روﺮﻣ ﻪﺑ هوﻼﻋ ﻦﯾا ﻞﺑﺎﻘﻣ رد تاﺮﺸﺣ و ﺎﻫ

ﯾﺪﺟ نﻮﯿﺳﻻﻮﻣﺮﻓ ﻪﺑ ﺪﻨﻣزﺎﯿﻧ زور ﺮﻫ و هﺪﺷ موﺎﻘﻣ مﻮﻤﺳ ياﺮﺑ ﺪ

نآ يدﻮﺑﺎﻧ ﯽﻣ ﺎﻫ ﺪﺷﺎﺑ . ﺰـﯿﻧ داﻮـﻣ ﻦـﯾا زا هدﺎﻔﺘـﺳا مﺪـﻋ ﺪـﻨﭼﺮﻫ

داﻮـﻣ و يزروﺎﺸﮐ تﻻﻮﺼﺤﻣ دﺎﯾز رﺎﯿﺴﺑ تﺎﻌﯾﺎﺿ زوﺮﺑ ﺐﺟﻮﻣ ﯽﻣ تﺎﻓآ ﻪﻠﻤﺣ ﺮﺛا ﺮﺑ ﯽﯾاﺬﻏ

ددﺮﮔ ] 3 .[

ﯽـﺷﻼﺗ ﺮﺿﺎﺣ ﺶﻫوﮋﭘ

ﺮﻀـﻣ ﺮـﯿﻏ و ﯽـﻌﯿﺒﻃ داﻮـﻣ زا هدﺎﻔﺘﺳا فﺪﻫ ﺎﺑ ﻪﮐ ﺖﺳا )

ﺮـﯿﻈﻧ

ﺲﻧﺎﺳا نآ ﻪـﯿﻟوا داﻮـﻣ ﻪـﮐ ﯽﻌﯿﺒﻃ يﺎﻫ د ﺎـﻫ

ﻪـﺑ رﻮﺸـﮐ ﻞـﺧاد ر

ﯽـﻣ لﺎﺼـﺤﺘﺳا ﻞـﺑﺎﻗ ﯽﮔدﺎـﺳ ﻪﺑ و هﺪﺷ ﺪﯿﻟﻮﺗ ﯽﻧاواﺮﻓ ﺪﻨـﺷﺎﺑ

( ،

ﺶـﮐ چرﺎـﻗ و مﻮﻤﺳ فﺬﺣ و ﯽﻨﯾﺰﮕﯾﺎﺟ ﺖﻬﺟ ﯽﯾﺎﯿﻤﯿـﺷ يﺎـﻫ

ﯽﺗادراو ) چرﺎـﻗ ﺪـﺷر لﺮـﺘﻨﮐ رد هدﺎﻔﺘﺳا درﻮﻣ هﺪـﻨﻨﮐﺪﯿﻟﻮﺗ يﺎـﻫ

ﻪﻧﺎﺧدﺮـﺳ تﺎﻌﯾﺎـﺿ ﺶﻫﺎـﮐ و كﺎﻧﺮﻄﺧ مﻮﻤﺳ

تﻻﻮﺼـﺤﻣ يا

يزروﺎﺸﮐ ( ﯽﻣ مﺎﺠﻧا دﻮﺷ ـﺳا ﺖـﯿﻠﺑﺎﻗ ﺖـﻘﯿﻘﺣ رد و يﺎـﻫ ﺲﻧﺎ

و ﺎﻫ هﻮﯿﻣ دﺎﺴﻓ رد ﻞﯿﺧد يﺎﻫ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﺒﻏ رد ﯽﻫﺎﯿﮔ هﻮﻬﻗ زوﺮﺑ زا يﺮﯿﮕﺸﯿﭘ رﻮﻈﻨﻣ ﻪﺑ تﺎﺠﯾﺰﺒﺳ ار ﯽـﻤﯾﺰﻧآ نﺪﺷ يا

ﺪﻨﮐ ﯽﻣ ﯽﺳرﺮﺑ .

ﺖﯾدوﺪـﺤﻣ ﻪـﺑ ﺖـﯾﺎﻨﻋ ﺎﺑ اﺬﻟ نوﺰـﻓازور يﺎـﻫ

شﺮﺘﺴــﮔ زا ﯽــﺷﺎﻧ ﯽــﺑوﺮﮑﯿﻣ ﺪــﺿ ﯽﯾﺎﯿﻤﯿــﺷ داﻮــﻣ زا هدﺎﻔﺘــﺳا ﯽﻣ ﺮﻈﻧ ﻪﺑ ﯽﯾوراد ﺖﻣوﺎﻘﻣ

ﺲﻧﺎﺳا ﺪﺳر

يﺎـﻫ ﺪـﺿ ﻊﺑﺎـﻨﻣ راﺮـﻓ

يرﺎـﻤﯿﺑ لﺮـﺘﻨﮐ و ﯽﮐارﻮﺧ داﻮﻣ ﻆﻔﺣ رد يﺮﺘﻬﺑ ﯽﺑوﺮﮑﯿﻣ

يﺎـﻫ

ﺪﻨﺷﺎﺑ ﯽﻧﺎﺴﻧا ]

4 .[

ﯽﻌﯿﺒﻃ تﺎﺒﯿﮐﺮﺗ زا هدﺎﻔﺘﺳا ﻪﺑ ﻞﯾﺎﻤﺗ هوﻼﻋ ﻪﺑ

فﺮﺼـﻣ رﺎﺸـﻓ ﺮـﺛا رد ﯽـﺑوﺮﮑﯿﻣ ﺪﺷر زا يﺮﯿﮔﻮﻠﺟ ياﺮﺑ هﺪـﻨﻨﮐ

ﯽﻧدوﺰﻓا زا هدﺎﻔﺘﺳا مﺪﻋ ﺎﯾ ﺶﻫﺎﮐ ﺖﻬﺟ و هﻮﯿﻣ رد ﯽﯾﺎﯿﻤﯿﺷ يﺎﻫ

يﺰﺒﺳ ﯾاﺰﻓا لﺎﺣ رد ﺎﻫ ﺖﺳا ﺶ

] 5 .[

ﺲﻧﺎﺳا ﯽﺑ ،ﯽﻌﯿﺒﻃ تﺎﺒﯿﮐﺮﺗ ﺎﻫ

هﺪﯿﭽﯿﭘ و ﮓﻧر

،ﺪﯿﺋﺪﻟآ ،ﻞﮑﻟا زا يا

و ﺮﺘﺳا ...

رد و هدﻮـﺑ دﻮـﺧ ﻪﺑ صﻮﺼﺨﻣ يﻮﺑ ياراد ﻪﮐ ﺪﻨﺘﺴﻫ ﻞﺑﺎﻗ ﯽﻋﻮﻨﺼﻣ تﺎﺒﯿﮐﺮﺗ

ﺶﻘﻧ نﺎﺴﻧا ﺖﻣﻼﺳ رد و هدﻮﺒﻧ سﺮﺘﺳد

ﺪﻧراد ﺖﯿﻤﻫا ﺰﺋﺎﺣ .

بآ زا ﺮـﺘﻤﮐ ﯽﻟﻮـﮑﻟﻮﻣ نزو تﺎـﺒﯿﮐﺮﺗ ﻦـﯾا

ﻨﻧﺎﻣ ﯽﻣ بآ ﺢﻄﺳ رد و ﻪﺘﺷاد ناﻮـﻨﻋ ﻪـﺑ نآ زا و هدﻮـﺑ راﺮـﻓ ،ﺪ

رﻮﺨﺑ ) سﻮﺘﭙﯿﻟﺎﮐا ﺲﻧﺎﺳا (

ﻢﻌﻃ ، ﯽﺘﻧآ ،اﺬﻏ هﺪﻨﻫد

ﺪـﺿ و ناﺪﯿﺴﮐا

هدﺎﻔﺘﺳا ﯽﺑوﺮﮑﯿﻣ ﯽﻣ يدﺎﯾز يﺎﻫ

ددﺮﮔ ] 6 .[

ﻦﺸﯾوآ ﯽﻤﻠﻋ مﺎﻧ ﺎﺑ Thymus L

ﯽـﮑﯾ زا ﺲﻨـﺟ يﺎـﻫ ﻢـﻬﻣ

هداﻮﻧﺎﺧ ﻋﺎﻨﻌﻧ ﺖﺳا نﺎﯿ ﻪﮐ زا ﻢﻬﻣ ﻦﯾﺮﺗ نﺎﻫﺎﯿﮔ ﯽﯾوراد رد ناﺮﯾا

ﯽﻣ و ﺪﺷﺎﺑ ﻪﺑ ﻞﯿﻟد ﻦﺘﺷاد د و ﺐﯿﮐﺮﺗ لﻮﻤﯿﺗ و لوﺮﮐاورﺎﮐ ياراد

صاﻮﺧ ﯽﯾوراد ﺮﯿﻈﻧ يدﺪﻌﺘﻣ ﺮﺛا

،ﺦـﻔﻧ ﺪـﺿ ،ﻢﺳﺎﭙﺳا ﺪﺿ ﺪـﺿ

،ﻢﺴﯿﺗﺎﻣور ﯽﻧﻮﻔﻋ ﺪﺿ

هﺪﻨﻨﮐ و ﺪﺿ يﻮـﻗ ﯽـﺑوﺮﮑﯿﻣ ﺖـﺳا

. رد

ﯽﻌﯿـﺳو تﺎـﻘﯿﻘﺤﺗ ﻦﺸـﯾوآ هﺎـﯿﮔ ﯽـﺑوﺮﮑﯿﻣ ﺪـﺿ تاﺮـﺛا ﻪﻨﯿﻣز لﺎﺜﻣ ماﻮﻨﻋ ﻪﺑ ﺖﺳا ﻪﺘﻓﺮﮔ ترﻮﺻ لﺎـﺳ رد نارﺎـﮑﻤﻫ و ﻖﻨﯿـﺳ

) 2003 ( نﺎﯿﺑ ﺪﻧدﻮﻤﻧ ﯾوآ ﻪﮐ يﺮﺘﮐﺎﺑ ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﮏﺨﯿﻣ و ﻦﺸ

ﯽﻣ ﺎﻫ ددﺮﮔ ] 7 .[

نارﺎـﮑﻤﻫ و ﻮـﺳﺎﺗ )

2000 ( ﻪـﮐ ﺪـﻧدﻮﻤﻧ نﺎـﯿﺑ

ﻞـﯿﻟد ﻪﺑ ﻦﺸﯾوآ ﯽﺑوﺮﮑﯿﻣ ﺪﺿ ﺖﯿﺻﺎﺧ لﺎـﻘﺘﻧا ﻦﺘـﻓر ﺖـﺳد زا

ﯽﻤــﺳﻼﭘﻮﺘﯿﺳ ءﺎﺸــﻏ ﺢﻄــﺳ رد يﺮﯾﺬــﭘ ذﻮــﻔﻧ و نوﺮــﺘﮑﻟا ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ ﺎﻫ

ﺪﺷﺎﺑ ﯽﻣ ] 8 .[

ﻤﻫ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻦﯿﻨﭽ

ﺮﯿﯿﻐﺗ ﺎﺑ

وﺮﮑﯿﻣ ءﺎﺸﻏ يﺮﯾﺬﭘ ذﻮﻔﻧ رد ،نﻮـﺗﺮﭘ دﻮـﺒﻤﮐ ﺐﺒـﺳ ﺎﻫ ﻢﺴﯿﻧﺎﮔرا

ددﺮـﮔ ﯽﻣ لﻮﻠﺳ ﯽﻠﺧاد ﻢﯿﺳﺎﺘﭘ و تﺎﻔﺴﻓ ]

9 .[

ﮏـﯾ لﺮﮐاورﺎـﮐ

و هدﻮﺑ ﯽﻨﭘﺮﺗ ﺐﯿﮐﺮﺗ دﻮﺟﻮﻣ ﺐﻟﺎﻏ هﺮﺛﻮﻣ هدﺎﻣ

نﺎﻫﺎﯿﮔ ﺲﻧﺎﺳا رد

ﻦﺸﯾوآ

، نﺎﯿﻧز و ﮏﺨﯿﻣ ﺖـﺳا

ﻪـﺑ ﻪـﮐ ﻞـﯿﻟد ﺎـﯾ ﺰﺘﻨـﺳ ﺶﻫﺎـﮐ

ءﺎﺸﻏ يﺮﯾﺬﭘ ذﻮﻔﻧ رد ﺮﯿﯿﻐﺗ نوﺪﺑ ﺰﯿﻟورﺪﯿﻫ فﺬﺣ ﺐﺒﺳ

ATP

يﺮﺘﮐﺎﺑ لﻮﻠﺳ ﻞﺧاد ﯽﻣ

ددﺮﮔ ] 10 .[

ﺮﯿﻈﻧ ﯽﻔﻠﺘﺨﻣ يﺎﻫ ﻢﯾﺰﻧآ ،ﺎﻫزاﺪﯿﺴـﮐاﺮﭘ

ﯽـﻠﭘ ،ﺎﻫزاﺮﺘـﺳا ﻦﯿـﺘﮑﭘ

ﺎﻫزاﺪﯿﺴـﮐا لﻮﻨﻓ ﯽﻠﭘ ،ﺎﻫزﺎﻧوﺮﺘﮐﻻﺎﮔ ﺎـﻫزﻼﻨﻓ و ﺎﻫزﺎﻧﮋﯿﺴـﮐاﻮﭙﯿﻟ ،

تﺎﺠﯾﺰﺒـﺳ و ﺎـﻫ هﻮـﯿﻣ ﯽـﻤﯾﺰﻧآ دﺎﺴﻓ لﻮﺌﺴﻣ و هدﻮـﺑ

ﺪـﻨﻧاﻮﺘﯿﻣ

(3)

تاﺮـﯿﯿﻐﺗ و ﻢـﻌﻃ ،ﺮﻄﻋ ،ﮓﻧر بﻮﻠﻄﻣﺎﻧ ﻪﯾﺰﺠﺗ ﻪﯿﻟوا يﺎﻬﺸﻨﮐاو ﻪﯾﺬﻐﺗ ﻧﻮﺷ ﺐﺒﺳ ار ﯽﺴﺣ و يا ﺪ

. ﻢﯾﺰـﻧآ ﮏـﯾ زاﺪﯿﺴـﮐاﺮﭘ ﻢﯾﺰﻧآ

ﺶﻨﮐاو زا يدﺎﯾز رﺎﻤﺷ و هدﻮﺑ راد ﻦﻫآ ﻪـﮐ ﺪﻨﮐ ﯽﻣ ﺰﯿﻟﺎﺗﺎﮐ ار ﺎﻫ

ار هﺪـﻨﻨﮐ ءﺎـﯿﺣا ﺶﻘﻧ نوﺮﺘﮑﻟا نداد ﺎﺑ نژورﺪﯿﻫ ﺪﯿﺴﮐاﺮﭘ نآ رد داﻮـﻣ رد ﮓـﻧر و ﻢـﻌﻃ ﺶﻫﺎﮐ ﺎﺑ نآ ﯽﺑﺮﺠﺗ طﺎﺒﺗرا و هدﺮﮐ ﺎﻔﯾا مﺎﺧ ﺖﺳا هﺪﺷ تﺎﺒﺛا ]

11 .[

ﻢﯾﺰـﻧآ ﻦﯾا ندﺮﮐ لﺎﻌﻓﺮﯿﻏ ﻦﯾاﺮﺑﺎﻨﺑ

ﻋ ﺶﯾاﺰـﻓا يراﺪـﻬﮕﻧ ﻦﯿﺣ رد ار تﺎﺠﯾﺰﺒﺳ و ﺎﻫ هﻮﯿﻣ يرﺎﺒﻧا ﺮﻤ

ﺪﻫﺪﯿﻣ ﺖﻈﻠﻏ و ﻻﺎﺑ ﯽﺗراﺮﺣ ﺖﻣوﺎﻘﻣ ﺮﻃﺎﺧ ﻪﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ. رد ﯽـﺗراﺮﺣ يﺮـﺑ ﻢﯾﺰـﻧآ ﺖـﯾﺎﻔﮐ ﺺﺧﺎﺷ ناﻮﻨﻋ ﻪﺑ نآ زا ،دﺎﯾز

دﻮـﺷ ﯽـﻣ هدﺎﻔﺘـﺳا ﺎـﻫ هﻮـﯿﻣ و ﺎﻫ يﺰﺒﺳ ﺐﻠﻏا .

ﻪـﺑ زاﺪﯿﺴـﮐاﺮﭘ

يﺎﻬﻤﯾﺰﻧآوﺰﯾا ترﻮﺻ )

اﺪﯿﺴـﮐاﺮﭘ نﻮﯿﺗﺎـﺗﻮﻠﮔ ﻞﻣﺎﺷ مﺮﮐﻮﺘﯿـﺳ ،ز

زاﺪﯿﺴـﮐاﺮﭘ ﺶـﯾدر سرﻮـﻫ ،زاﺪﯿﺴـﮐاﺮﭘﻮﺘﮐﻻ ،زاﺪﯿﺴـﮐاﺮﭘ ﯽﺳ

،

ﻪـﺑ توﺎـﻔﺘﻣ ﺖﯿﺳﺎﺴﺣ ﺎﺑ زاﺪﯿﺴﮐاﺮﭘ نژورﺪﯿﻫ و زاﺪﯿﺴﮐاﺮﭘﻮﻟﺎﻫ ﯽــﺗراﺮﺣ نﻮﯿــﺳارﻮﺗﺎﻧد )

ﺮــﺑاﺮﺑ رد راﺪــﯾﺎﭘﺎﻧ و موﺎــﻘﻣ يﺎــﻬﻣﺮﻓ

تراﺮﺣ ( هﻮﯿﻣ و ﺎﻬﯾﺰﺒﺳ رد ﺖﺳا هﺪﺷ ﺖﻓﺎﯾ ﺎﻫ

. هﺪﻤﻋ ﺪﻨﯾآﺮﻓ ﻪﺳ

اﺮﭘ يزﺎﺳ لﺎﻌﻓﺮﯿﻏ رد ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ﻞﻣﺎـﺷ زاﺪﯿﺴﮐ

1 - ﮏـﯿﮑﻔﺗ

ﮏﯿﺗﺎﺘﺳوﺮﭘ هوﺮﮔ Heme

ﻢﯾﺰﻧآﻮﻟﺎﻫ زا )

ﻢﯾﺰـﻧآ لﺎـﻌﻓ ﻢﺘﺴـﯿﺳ (

،

2 - ﻢﯾﺰﻧآﻮــﭘآ ﺐــﯿﮐﺮﺗ ﺮــﯿﯿﻐﺗ )

ﻢﯾﺰــﻧآ ﯽــﻨﯿﺌﺗوﺮﭘ ﺖﻤﺴــﻗ (

و 3 -

ﻪﯾﺰﺠﺗ تاﺮﯿﯿﻐﺗ ﺖﺳا ﮏﯿﺗﺎﺘﺳوﺮﭘ هوﺮﮔ يا

] 12 .[

تﺎﺠﯾﺰﺒـﺳ زا يدﺎـﯾز داﺪﻌﺗ هرﺎﺼﻋ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ ]

17 - 13 .[

لﺎـﻌﻓﺮﯿﻏ ﺖﻬﺟ يزﺎـﺳ

شور زا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ تراﺮﺣ ﺮﯿﻈﻧ ﯽﻔﻠﺘﺨﻣ يﺎﻫ

ﺶﻫﺎﮐ ،ﯽﻫد

ﯽﺑآ ﺖﯿﻟﺎﻌﻓ ﺎﯾ pH )

aw

( ﯽﻧدوﺰﻓا ﺎﯾ و ﯽﯾﺎﯿﻤﯿﺷ يﺎﻫ

) يد ﺪﯿﺴـﮐا

دﺮﮔﻮﮔ ( ﯽﻣ هدﺎﻔﺘﺳا شور ﻦﯾا ﻪﮐ دﻮﺷ

ﺖﯾدوﺪﺤﻣ ياراد ﺎﻫ يﺎـﻫ

ﯽﻣ دﻮﺧ ﻪﺑ صﻮﺼﺨﻣ ﺪﻨﺷﺎﺑ

. فﺮﺼﻣ ﻞﯾﺎﻤﺗ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ نﺎﮔﺪـﻨﻨﮐ

ﺼﺤﻣ زا هدﺎﻔﺘﺳا ﻪﺑ هﺪﻨﻨﮐﺪﯿﻟﻮﺗ ،يروآﺮﻓ ﻞﻗاﺪﺣ ﺎﺑ ﯽﯾاﺬﻏ تﻻﻮ

ﺎﻫ

شور لﺎﺒﻧد ﻪﺑ

يراﺪـﻬﮕﻧ ﺮـﻤﻋ ﺶﯾاﺰـﻓا ياﺮـﺑ ﻦﯾﺰﮕﯾﺎـﺟ يﺎـﻫ

ﺪﻨﺘﺴﻫ تﻻﻮﺼﺤﻣ .

ﯽـﺑوﺮﮑﯿﻣ ﺪـﺿ ﺖﯿـﺻﺎﺧ ﻪـﮑﻨﯾا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ

ﺲﻧﺎﺳا ﺮﯿﺛﺎﺗ ﻪﻟﺎﻘﻣ ﻦﯾا رد ﺖﺳا هﺪﯿﺳر تﺎﺒﺛا ﯽﻫﺎﯿﮔ يﺎﻫ ﺲﻧﺎﺳا هﻮـﯿﻣ دﺎﺴﻓ رد ﻞﯿﺧد يﺎﻫ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ﺮﺑ ﯽﻫﺎﯿﮔ يﺎﻫ و ﺎﻫ ﺪﺷ ﯽﺳرﺮﺑ تﺎﺠﯾﺰﺒﺳ .

ﺶﯿﭘ ﻪﮐ رﻮﻄﻧﺎﻤﻫ ﺪـﺷ ﺮﮐذ ﺮﺗ

ﻢﯾﺰﻧآ

ﻢﯾﺰﻧآ زا ﯽﮑﯾ زاﺪﯿﺴﮐاﺮﭘ ﺖﻓﺎﺑ رد ﻢﻬﻣ يﺎﻫ

ﻪـﮐ ﺖﺳا ﯽﻫﺎﯿﮔ يﺎﻫ

نژورﺪﯿﻫ ﺪﯿﺴﮐاﺮﭘ ﺎﺑ )

ﻪﻧﮋﯿﺴﮐا بآ (

ﺲﮑﻠﭙـﻤﮐ ﺪـﯿﻟﻮﺗ و ﺐـﯿﮐﺮﺗ

ﯽﻣ ﯽﻟﺎﻌﻓ لﻮـﮑﻟﻮﻣ زا يرﺎﯿﺴﺑ ﺎﺑ ﺶﻨﮐاو مﺎﺠﻧا ﻪﺑ ردﺎﻗ ﻪﮐ ﺪﯾﺎﻤﻧ -

ﯽﻣ نوﺮﺘﮑﻟا هﺪﻨﻫد يﺎﻫ ﺪﺷﺎﺑ

. يراﺪﻘﻣ ﻦﯿﻨﭽﻤﻫ ﺮـﺛا رد نﮋﯿﺴـﮐآ

ﯽﻟﻮﻨﻓ تﺎﺒﯿﮐﺮﺗ ﺎﺑ ﺪﻧاﻮﺗ ﯽﻣ ﻪﮐ دﻮﺷ ﯽﻣ ﺪﯿﻟﻮﺗ ﻢﯾﺰﻧآ ﻦﯾا ﺖﯿﻟﺎﻌﻓ ﺪـﻨﯾﺎﻤﻧ ﺰﯿﻟﺎـﺗﺎﮐ ار ﯽـﻤﯾﺰﻧآ نﺪﺷ يا هﻮﻬﻗ و ﺶﻨﮐاو هﺎﯿﮔ ﻞﺧاد .

فﺬـﺣ ﺎـﺑ ﺪـﻧاﻮﺗ ﯽﻣ ناﺪﯿﺴﮐا ﯽﺘﻧآ تﺎﺒﯿﮐﺮﺗ زا هدﺎﻔﺘﺳا ﻦﯾاﺮﺑﺎﻨﺑ ﺖﻌﻧﺎﻤﻣ ﯽﻤﯾﺰﻧآ نﺪﺷ يﺎﻫ ﻮﻬﻗ ﺶﻨﮐاو زوﺮﺑ زا يﺪﯿﻟﻮﺗ نﮋﯿﺴﮐا

ﺪﻨﻨﮐ . اد نﺎﻫﺎﯿﮔ ﺖـﺳا ﺮﺗﻻﺎـﺑ ﺎـﻬﻧآ يﺎﻫ ﻞﻨﻓ ﯽﻠﭘ راﺪﻘﻣ ﻪﮐ ﯽﺋور

ﺪﻨﺘﺴـﻫ يﺮﺘﺸـﯿﺑ ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ ﺖﯿﺻﺎﺧ ياراد .

ﯽـﺘﻧآ صاﻮـﺧ

نآ ﺮﺛﻮـﻣ داﻮﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻒﻠﺘﺨﻣ ﯽﻫﺎﯿﮔ يﺎﻫ ﺲﻧﺎﺳا ﯽﻧاﺪﯿﺴﮐا ﯽﻫﺎـﯿﮔ ﺲﻧﺎﺳا ﮏﯾ ﺮﺛﻮﻣ داﻮﻣ رد فﻼﺘﺧا ﻦﯾا ﻪﮐ هدﻮﺑ توﺎﻔﺘﻣ ﺖﺳا هﺪﻫﺎﺸﻣ ﻞﺑﺎﻗ حﻮﺿو ﻪﺑ ﺰﯿﻧ صﺎﺧ .

ﻦﯿﻠﮐ و اﺪﻤﻫ )

1990 (

ﻮﻨﻋ داﻮـﻣ ﺪـﺿ ﺮـﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ رﺎﺘﻓر رد فﻼﺘﺧا ﺪﻧدﻮﻤﻧ نا هداد ﺖﺒﺴـﻧ ﺎـﻫ ﻢﯾﺰـﻧآ وﺰﯾا رﻮﻀﺣ ﻪﺑ ﺪﻧاﻮﺗ ﯽﻣ ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ

دﻮﺷ ] 18 .[

ﺶﻫوﮋﭘ ﻦﯾا مﺎﺠﻧا زا فﺪﻫ ﺲﻧﺎـﺳا ﯽﯾﺎﻧاﻮﺗ ﯽﺳرﺮﺑ

ﻦﺸﯾوآ يزﺎﺳ لﺎﻌﻓﺮﯿﻏ رد ﻢﯾﺰﻧآ

ﺰﺒـﺳ ﺎـﯿﺑﻮﻟ و بﺮـﺗ زاﺪﯿﺴﮐاﺮﭘ

ﺖﺳا هدﻮﺑ . ﯽﻧاﺪﯿﺴـﮐا ﯽـﺘﻧآ ﺖﯿﺻﺎﺧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ يﺎـﻫ ﺲﻧﺎـﺳا

داﻮـﻣ ﻦﯾا ﺮﯿﺛﺎﺗ ﺶﯾﺎﻣزآ رد ﯽﺷﻼﺗ ﺪﻧاﻮﺗ ﯽﻣ ﺶﻫوﮋﭘ ﻦﯾا ﯽﻫﺎﯿﮔ ﯽـﻤﯾﺰﻧآ نﺪـﺷ يا هﻮـﻬﻗ رد ﺮﺛﻮﻣ يﺎﻫ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ رد ) زاﺪﯿﺴﮐاﺮﭘ ( هﻮـﯿﻣ ﻢـﻌﻃ و ﮓـﻧر ﻆﻔﺣ نﺎﮑﻣا ﻪﺠﯿﺘﻧ رد و و ﺎـﻫ

ناﻮﻨﻋ ﻪﺑ ﻢﯾﺰﻧآ ﻦﯾا يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ﻖﯾﺮﻃ زا ﯽﮐارﻮﺧ تﺎﺠﯾﺰﺒﺳ ﯽﻠﭘ يﺎﻫ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ زﺎﯿﻧ ﺶﯿﭘ ﺪﺷﺎﺑ زاﺪﯿﺴﮐا ﻞﻨﻓ

.

2 - ﺎﻫ شور و داﻮﻣ

بﺮﺗ ﺶﻫوﮋﭘ ﻦﯾا مﺎﺠﻧا ﺖﻬﺟ هﺎﯿﺳ

و هﻮﯿﻣ رازﺎﺑ زا ﺰﺒﺳ ﺎﯿﺑﻮﻟ و

نﺎﮔﺮﮔ نﺎﺘﺳﺮﻬﺷ يﺰﺒﺳ )

ناﺮﯾا ( ﺪﺷ ﻪﯿﻬﺗ . ﻪﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا

ﺲﻧﺎﺳا ﺞﯾرﺎﺑ ﺖﮐﺮﺷ زا ﯽﻌﯿﺒﻃ ناﺪﯿﺴﮐا ﯽﺘﻧآ يوﺎﺣ ﻊﺒﻨﻣ ناﻮﻨﻋ ﻦﯿﯾﻮﺗ زا هدﺎﻔﺘﺳا ﺎﺑ و ﻪﯿﻬﺗ 80

، ﺖﻈﻠﻏ يﺎﻫ 50 ، 75 ، 100 ، 200

(g/100 ml) ﺪﯾدﺮﮔ ﻪﯿﻬﺗ نآ زا

.

2 - 1 - ﺰﺒﺳ ﺎﯿﺑﻮﻟ و بﺮﺗ ﯽﻤﯾﺰﻧآ هرﺎﺼﻋ ﻪﯿﻬﺗ

رﻮﻈﻨﻣ ﻦﯾا ياﺮﺑ 10

ﻦﻤﺿ و ﻦﯾزﻮﺗ ﺮﻈﻧ درﻮﻣ يﺰﺒﺳ زا مﺮﮔ

ندوﺰﻓا 30 ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟ ﯽﻠﯿﻣ )

4 داﺮﮔ ﯽﺘﻧﺎﺳ ﻪﺟرد (

دﺮﺧ

تﺪﻣ ﻪﺑ و ﺪﯾدﺮﮔ 15

يﺎﻣد رد ﻪﻘﯿﻗد 4

داﺮﮔ ﯽﺘﻧﺎﺳ ﻪﺟرد

ژﻮﻔﯾﺮﺘﻧﺎﺳ )

10000 ﻪﻘﯿﻗد رد رود (

ﺪﺷ يوﺎﺣ ﻪﮐ ﯽﯾور ﻊﯾﺎﻣ و

ﯽﻣ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ هدﺎﻔﺘﺳا درﻮﻣ ﯽﻤﯾﺰﻧآ هرﺎﺼﻋ ناﻮﻨﻋ ﻪﺑ ﺪﺷﺎﺑ

ﺖﻓﺮﮔ راﺮﻗ ]

13 .[

2 - 2 - ﺶﻨﮐاو طﻮﻠﺨﻣ

طﻮﻠﺨﻣ ﻞﻣﺎﺷ اﺮﺘﺴﺑﻮﺳ طﻮﻠﺨﻣ 10

لﻮﮐﺎﯾﺎﮔ ﺮﺘﯿﻟ ﯽﻠﯿﻣ 1

% ، 10

نژورﺪﯿﻫ ﺪﯿﺴﮐاﺮﭘ ﺮﺘﯿﻟ ﯽﻠﯿﻣ 3

% و 100 تﺎﻔﺴﻓ ﺮﻓﺎﺑ ﺮﺘﯿﻟ ﯽﻠﯿﻣ

ﻪﮐ دﻮﺑ ﻢﯾﺪﺳ يور ﺶﻨﮐاو طﻮﻠﺨﻣ pH

5/

6 ﺪﺷ ﻢﯿﻈﻨﺗ ]

18 .[

2 -

3 -

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺮﯿﮔ هزاﺪﻧا

(4)

ﻦﯿﻠﮐ و اﺪﻤﻫ شور ﻖﺒﻃ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﺠﻨﺳ )

1990 ( مﺎﺠﻧا

ﺪﯾدﺮﮔ ] 18 .[

ﺖﻬﺟ ﺖﻈﻠﻏ رد هدﺎﻔﺘﺳا درﻮﻣ ﺲﻧﺎﺳا ﺮﺛا ﯽﺳرﺮﺑ

رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ،يدﺮﺑرﺎﮐ يﺎﻫ 25

ﯽﺘﻧﺎﺳ ﻪﺟرد

رد ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا ﺎﺑ و داﺮﮔ 470

ﺎﺑ ﺮﺘﻣﻮﻧﺎﻧ لﻮﮐﺎﯾﺎﮔ زا هدﺎﻔﺘﺳا

) كﺮﻣ ( نژورﺪﯿﻫ ﺪﯿﺴﮐاﺮﭘ و اﺮﺘﺴﺑﻮﺳ ناﻮﻨﻋ ﻪﺑ )

كﺮﻣ ( ناﻮﻨﻋ ﻪﺑ

هزاﺪﻧا نژورﺪﯿﻫ هﺪﻨﻫد ﺪﺷ يﺮﯿﮔ

. رﻮﻈﻨﻣ ﻦﯾﺪﺑ 87

/2 ﺮﺘﯿﻟ ﯽﻠﯿﻣ

،اﺮﺘﺴﺑﻮﺳ طﻮﻠﺨﻣ 1/

0 ﯽﻠﯿﻣ و مﺎﺧ هرﺎﺼﻋ ﺮﺘﯿﻟ 03

/0 ﺮﺘﯿﻟ ﯽﻠﯿﻣ

ﺎﻌﻤﺟ ﻪﮐ ﺮﻄﻘﻣ بآ 3

ﯽﻠﯿﻣ بﺬﺟ و ﻞﻘﺘﻨﻣ توﺎﮐ ﻪﺑ دﻮﺑ ﺮﺘﯿﻟ

ﻪﻧﻮﻤﻧ ترﻮﺻ ﻪﺑ ﺎﻫ جﻮﻣ لﻮﻃ رد ﯽﮑﯿﺘﻨﯿﺳ

470 تﺪﻣ ﻪﺑ ﺮﺘﻣﻮﻧﺎﻧ

400 ﺪﺷ ﺖﺋاﺮﻗ ﻪﯿﻧﺎﺗ .

ﺲﻧﺎﺳا ﺮﺛا ﯽﺳرﺮﺑ ﺖﻬﺟ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﯽﻫﺎﯿﮔ

ﯽﻤﯾﺰﻧآ هرﺎﺼﻋ و اﺮﺘﺴﺑﻮﺳ طﻮﻠﺨﻣ ﻪﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ 03

/0

ﯽﻠﯿﻣ و ﻪﻓﺎﺿا ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ زا ﺮﺘﯿﻟ

جﻮﻣ لﻮﻃ رد ﯽﮑﯿﺘﻨﯿﺳ ترﻮﺻ ﻪﺑ ﺎﻫ ﻪﻧﻮﻤﻧ بﺬﺟ 470

ﺮﺘﻣﻮﻧﺎﻧ

ﺮﻗ ﺪﺷ ﺖﺋا ] 18 .[

يﺮﯿﮔ هزاﺪﻧا ياﺮﺑ يﺪﻌﺑ ﮥﻠﺣﺮﻣ رد ﯽﺳرﺮﺑ

لﺎﻌﻓ ﺮﯿﻏ ﺮﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺮﯿﺛﺎﺗ يزﺎﺳ

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﻓﺎﺑ رد

،يﺰﺒﺳ يراﺪﻘﻣ ﻟﻮﻠﺤﻣ رد ار ﺮﻈﻧ درﻮﻣ يﺰﺒﺳ زا ﺎﺑ ﯽ

ﺖﻈﻠﻏ يﺎﻫ

ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ )

50 ، 75 ، 100 و 200 (g/100 ml ﻪﺑ

تﺪﻣ 1 رو ﻪﻃﻮﻏ ﻪﻘﯿﻗد و هدﻮﻤﻧ

زا ﺲﭘ 1 ﺖﻋﺎﺳ شور ﻖﺑﺎﻄﻣ

و هﺪﺷ مﺎﺠﻧا يﺮﯿﮔ هرﺎﺼﻋ ﻞﺒﻗ ترﻮﺻ ﻪﺑ ﺎﻫ ﻪﻧﻮﻤﻧ بﺬﺟ

جﻮﻣ لﻮﻃ رد ﯽﮑﯿﺘﻨﯿﺳ 470

تﺪﻣ ﻪﺑ ﺮﺘﻣﻮﻧﺎﻧ 400

ﺖﺋاﺮﻗ ﻪﯿﻧﺎﺛ

ﺪﺷ .

2 - 4 - يرﺎﻣآ يﺎﻫ ﻪﯾﺰﺠﺗ

مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ و ﯽﻓدﺎﺼﺗ ﻼﻣﺎﮐ حﺮﻃ ﺐﻟﺎﻗ رد ﺶﯾﺎﻣزآ ﻦﯾا يرﺎﻣآ راﺰﻓا )

2001 ( ﺪﺷ مﺎﺠﻧا SAS . ﺎﻫ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﺖﻬﺟ

نﻮﻣزآ زا ﺢﻄﺳ رد ﻦﮑﻧاد يا ﻪﻨﻣاد ﺪﻨﭼ

5 % ﻢﺳر ياﺮﺑ و

راﺰﻓا مﺮﻧ زا ﺎﻫرادﻮﻤﻧ Excel

) 2003 ( ﺪﺷ هدﺎﻔﺘﺳا .

3 - ﺚﺤﺑ و ﺞﯾﺎﺘﻧ

بﺮﺗ مﺎﺧ هرﺎﺼﻋ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ناﺰﯿﻣ هﺎﯿﺳ

ﺎﯿﺑﻮﻟ و

ﺰﺒﺳ ﻪﺑ ﻪﮐ ﺪﺷ يﺮﯿﮔ هزاﺪﻧا ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا هﺎﮕﺘﺳد ﻂﺳﻮﺗ

ﺎﺑ ﺮﺑاﺮﺑ ﺐﯿﺗﺮﺗ 4625

و 900 ) يﺰﺒﺳ مﺮﮔ ×

ﯿﻧﺎﺛ ﻪ / ﺪﺣاو ﺖﯿﻟﺎﻌﻓ (

ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﺟﻮﺗ ﻞﺑﺎﻗ ناﺰﯿﻣ هﺪﻨﻫد نﺎﺸﻧ ﻦﯾا ﻪﮐ دﻮﺑ بﺮﺗ رد زاﺪﯿﺴﮐاﺮﭘ هﺎﯿﺳ

ﺪﺷﺎﺑ ﯽﻣ ﺰﺒﺳ ﺎﯿﺑﻮﻟ و .

ﻪﺑ طﻮﺑﺮﻣ ﺞﯾﺎﺘﻧ

ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ ﺮﯿﺛﺎﺗ ﻦﺸﯾوآ

ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ

بﺮﺗ مﺎﺧ هرﺎﺼﻋ رد زاﺪﯿﺴﮐاﺮﭘ هﺎﯿﺳ

رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﺰﺒﺳ ﺎﯿﺑﻮﻟ و

لواﺪﺟ ) 1 ( و ) 3 ( ﺷ ﻪﺋارا لواﺪﺟ رد ﻪﮐ ﻢﻫ رﻮﻄﻧﺎﻤﻫ و ﺖﺳا هﺪ

) 2 ( و ) 4 ( درﻮﻣ يﺎﻫ ﺖﻈﻠﻏ ﯽﻣﺎﻤﺗ رد ﻦﺸﯾوآ دﻮﺷ ﯽﻣ هﺪﻫﺎﺸﻣ

هدﺎﻔﺘﺳا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺮﺑ بﺮﺗ

هﺎﯿﺳ ﺚﻋﺎﺑ و ﻪﺘﺷاد ﺖﺒﺜﻣ ﺮﯿﺛﺄﺗ

ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ نآ

ﺪﯾدﺮﮔ . هدﻮﻤﻧ ﻞﻤﻋ ﺲﮑﻋﺮﺑ ﺰﺒﺳ ﺎﯿﺑﻮﻟ رد ﺎﻣا

و هدﺎﻔﺘﺳا درﻮﻣ يﺎﻫ ﺖﻀﻠﻏ ﯽﻣﺎﻤﺗ رد ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﺐﺟﻮﻣ

ﯾﺰﻧآ ﺖﺳا هﺪﺷ زاﺪﯿﺴﮐاﺮﭘ ﻢ ﻪﮐ

ﻦﯾا ﺶﯾاﺰﻓا ﯽﻣ ﺪﻧاﻮﺗ ﻪﺑ ﺖﻠﻋ

ﺶﯾاﺰﻓا ياﺮﺘﺴﺑﻮﺳ سﺮﺘﺳد رد

ﻢﯾﺰﻧآ و ﻢﮐ نﺪﺷ ﺮﺛا ﯽﮔﺪﻧرادزﺎﺑ

ﻢﯾﺰﻧآ رد ﺖﻈﻠﻏ يﺎﻫ توﺎﻔﺘﻣ ﺲﻧﺎﺳا ﺪﺷﺎﺑ ﻦﺸﯾوآ ]

14 .[

زا

ﯽﻔﻠﺘﺨﻣ يﺎﻫ ﻢﯾﺰﻧآوﺰﯾا ياراد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﻪﮐ ﺎﺠﻧآ )

زا ﺶﯿﺑ

1000 ﻢﯾﺰﻧآ وﺰﯾا (

توﺎﻔﺗ ﺮﮕﯾﺪﮑﯾ ﺎﺑ ﻪﮐ ﺖﺳا ﯽﺳﺎﺳا يﺎﻫ

ﺪﻧراد

؛ ﻪﺑ ﻢﯾﺰﻧآ ﻦﯾا ﺖﯿﺳﺎﺴﺣ ﺰﯿﻧ و ﺖﯿﻟﺎﻌﻓ راﺪﻘﻣ سﺎﺳا ﻦﯾا ﺮﺑ

ﺖﺳا توﺎﻔﺘﻣ ﻒﻠﺘﺨﻣ ﻞﻣاﻮﻋ .

رﺎﺘﻓر ﺪﯾﻮﻣ ﺰﯿﻧ ﻖﯿﻘﺤﺗ ﻦﯾا ﺞﯾﺎﺘﻧ

رﺎﻤﯿﺗ ﮏﯾ ﻪﺑ ﻢﯾﺰﻧآ ﻦﯾا توﺎﻔﺘﻣ )

ﻦﺸﯾوآ ﺲﻧﺎﺳا (

عﻮﻧ سﺎﺳا ﺮﺑ

دﻮﺑ ﯽﻤﯾﺰﻧآ ﻊﺒﻨﻣ .

ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻪﮑﯾرﻮﻃ ﻪﺑ

بﺮﺗ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ هﺎﯿﺳ

ﺪﯾﺪﺸﺗ ﺚﻋﺎﺑ ﺎﻣا دﻮﺑ هﺪﺷ

دﻮﺑ هﺪﺷ ﺰﺒﺳ ﺎﯿﺑﻮﻟ رد ﻢﯾﺰﻧآ ﻦﯾا ﺖﯿﻟﺎﻌﻓ .

ناﻮﺗ ﯽﻣ ﺖﻘﯿﻘﺣ رد

درﻮﻣ يﺰﺒﺳ ود رد ﻢﯾﺰﻧآ عﻮﻧ توﺎﻔﺗ ﺮﺑ هوﻼﻋ ﻪﮐ دﻮﻤﻧ نﺎﯿﺑ ﺰﺒﺳ ﺎﯿﺑﻮﻟ رد يﺮﮕﯾد هﺪﻨﻨﮐ ﺖﻌﻧﺎﻤﻣ ﻞﻣاﻮﻋ ﺖﺳا ﻦﮑﻤﻣ ﻪﻌﻟﺎﻄﻣ ار نآ ﺮﺛا و هداد ﺶﻨﮐاو ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺎﺑ ﻪﮐ ﺪﺷﺎﺑ ﻪﺘﺷاد دﻮﺟو ﺰﻧآ ﺮﺑ ﻪﯾﺰﺠﺗ ﺐﺟﻮﻣ ﯽﺘﺣ ﺎﯾ ﺪﺷﺎﺑ هداد ﺶﻫﺎﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾ

رد ﻪﮐ ﺪﺷﺎﺑ هﺪﺷ ﺮﮕﯾد لﺎﻌﻓ ﯽﻟﻮﻨﻓ تﺎﺒﯿﮐﺮﺗ ﻪﺑ ﺲﻧﺎﺳا

ﺶﻘﻧ ﻪﯿﺒﺷ ﻪﻧﮋﯿﺴﮐا بآ ﺎﺑ ﺶﻨﮐاو ﮔ

هدﻮﻤﻧ ﺎﻔﯾا ار لﻮﮐﺎﯾﺎ

ناﺰﯿﻣ و ار ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺪﻨﻫد نﺎﺸﻧ ﺮﺘﺸﯿﺑ

] 17 - 14 .[

Table 1 Effect of different concentrations of thyme essential oil on peroxidase activity (gram of vegetable × second / unit of activity) of crude extracts of selected vegetables

thyme essential oil concentration (g/100 ml) Peroxidase enzyme

sources 0 50 75 100 200

Black Spanish radish 4625^a 2655^b 2550^b 2015^c 2162^c

Green bean 51^c 708^a 551.6^b 579^b 690^a

*Superscript letters indicate that means with the same letters designation in a raw are not significantly different at P<0.05.

(5)

Table 2 Effect of different concentrations of thyme essential oil on percent relative peroxidase activity (gram of vegetable × second / unit of activity) of crude extracts of selected vegetables.

sources 0 50 75 100 200

Black Spanish radish 4625^a 42.59^c 44.86^c 56.43^b 53.24^b

Green bean 51^c -1288.23^a -981.56^b -1035.2^b -1252.94^a

*Superscript letters indicate that means with the same letters designation in a raw are not significantly different at P<0.05.

*Negative sign mean that proxidase activity increased more than blank sample (crude extracts).

Table 3 Effect of dipping in different concentrations of thyme essential oil on peroxidase activity (gram of vegetable × second / unit of activity) of selected vegetables

sources 0 50 75 100 200

Black Spanish radish 4625^d 815^b 960^a 720^c 975^a

Green bean 51^d 142.5^c 257.5^b 325^a 151.25^c

Superscript letters indicate that means with the same letters designation in a raw are not significantly different at P<0.05.

Table 4 Effect of dipping in different concentrations of thyme essential oil on percent relative peroxidase activity of selected vegetables

sources 0 50 75 100 200

Black Spanish radish 4625^a 82.37^c 79.24^d 84.43^b 78.91^d

Green bean 51^c -179.41^c -404.90^b -537.25^a -196.56^c

Superscript letters indicate that means with the same letters designation in a raw are not significantly different at P<0.05.

Negative sign mean that proxidase activity increased more than blank sample (crude extracts).

3 - 1 - ﺎﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﺠﻨﺳ

هرﺎﺼﻋ ﻪﺑ ﺲﻧﺎﺳا ﻢﯿﻘﺘﺴﻣ ندوﺰﻓا

ﻞﮑﺷ ) 1 ( ﺖﻈﻠﻏ ﺮﯿﺛﺎﺗ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ هرﺎﺼﻋ

بﺮﺗ هﺎﯿﺳ ﺪﻫد ﯽﻣ نﺎﺸﻧ ار .

ﺎﺠﻧآ زا ﻪﮐ

راﺪﻘﻣ و ﺪﺷﺎﺑ ﯽﻣ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﮕﻧﺎﯿﺑ بﺬﺟ ﯽﻨﺤﻨﻣ ﺐﯿﺷ ﺷ ﻪﺒﺳﺎﺤﻣ زا ﺖﯿﻟﺎﻌﻓ ﯿ

ﻦﯾاﺮﺑﺎﻨﺑ دﻮﺷ ﯽﻣ ﻪﺒﺳﺎﺤﻣ ﯽﻨﺤﻨﻣ ﺐ

،دﻮﺷ ﯽﻣ هﺪﻫﺎﺸﻣ ﻪﮐ رﻮﻄﻧﺎﻤﻫ ﺎﻫ ﺖﻈﻠﻏ ﻪﯿﻠﮐ رد ﻦﺸﯾوآ ﺲﻧﺎﺳا

رد ﻪﮑﯾرﻮﻃ ﻪﺑ ﺖﺳا هﺪﺷ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺚﻋﺎﺑ ﯽﻣ ﻪﻈﺣﻼﻣ ﻞﮑﺷ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺎﻫ ﯽﻨﺤﻨﻣ مﺎﻤﺗ دﻮﺷ

زاﺪﯿﺴﮐاﺮﭘ

ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﺖﺤﺗ ﻦﺸﯾوآ

ﺖﻈﻠﻏ ﺎﺑ يﺮﺘﻤﮐ ﺐﯿﺷ ﺺﺨﺸﻣ يﺎﻫ

ﻪﺑ ﺖﺒﺴﻧ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺎﻫ ﯽﻨﺤﻨﻣ ﻪﺘﺷاد بﺮﺗ ﮥﯿﻟوا

ﯽﻣ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺮﮕﻧﺎﯿﺑ ﻪﮐ ﺪﺷﺎﺑ

. زا ﻪﮐ ﺪﻨﭼﺮﻫ

نﺎﻣز يﺎﻫ 50 ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ ﺮﯿﺛﺎﺗ ﺪﺳر ﯽﻣ ﺮﻈﻧ ﻪﺑ ﺪﻌﺑ ﻪﺑ ﻪﯿﻧﺎﺛ

نآ ﯽﮔﺪﻨﻨﮐ ﺖﻌﻧﺎﻤﻣ و ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺮﺑ

ﻢﯾﺰﻧآ ﺶﻫﺎﮐ زاﺪﯿﺴﮐاﺮﭘ

ﺪﺷﺎﺑ ﻪﺘﻓﺎﯾ .

0 0.5 1 1.5 2 2.5

0 50 100 150

absorption spectrophotometry

time (second)

thyme 50 thyme 75

thyme 100 thyem 200

black Spanish radish

Fig 1 Effects of different concentrations of thyme essential oil on peroxidase enzymatic activity of

black Spanish radish crude extract

ﻞﮑﺷ ) 2 ( ﺖﻈﻠﻏ ﺮﯿﺛﺎﺗ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ هرﺎﺼﻋ

ﺪﻫد ﯽﻣ نﺎﺸﻧ ار ﺰﺒﺳ ﺎﯿﺑﻮﻟ .

ﻪﮐ رﻮﻄﻧﺎﻤﻫ

ﯽﻣ هﺪﻫﺎﺸﻣ ﺎﻫ ﺖﻈﻠﻏ ﻪﯿﻠﮐ رد ﻦﺸﯾوآ ﺲﻧﺎﺳا دﻮﺷ

ﺚﻋﺎﺑ ﺶﯾاﺰﻓا

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﯽﻨﺤﻨﻣ رد ﯽﻬﺟﻮﺗ ﻞﺑﺎﻗ هﺪﺷ

ﻪﺑ ﺖﺳا

(6)

ﻪﮑﯾرﻮﻃ د ،ﻦﺸﯾوآ ﺲﻧﺎﺳا يﺎﻫ ﺖﻈﻠﻏ ﻪﯿﻠﮐ ر ﺖﯿﻟﺎﻌﻓ ﯽﻨﺤﻨﻣ

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺪﻗﺎﻓ ﺎﯿﺑﻮﻟ يﺎﻫ هرﺎﺼﻋ زا ﺮﺘﺸﯿﺑ

ﯽﻣ ﺪﺷﺎﺑ . ﺖﺤﺗ ﺰﺒﺳ ﺎﯿﺑﻮﻟ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﯽﻟﻮﻨﻓ داﻮﻣ نﺪﺷ ﺐﯿﮐﺮﺗ ﺖﻠﻋ ﻪﺑ ﺪﻧاﻮﺗ ﯽﻣ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺮﯿﺛﺎﺗ

يا هﻮﻬﻗ ﮓﻧر دﺎﺠﯾا و ﻪﻧﮋﯿﺴﮐا بآ ﺎﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻪﮐ ﺪﺷﺎﺑ

ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺮﮕﯾد ترﺎﺒﻋ ﻪﺑ ﺖﺳا هﺪﺷ بﺬﺟ ﺶﯾاﺰﻓا ﺚﻋﺎﺑ ﺒﺳ ﺎﯿﺑﻮﻟ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺖﺳا ﻪﺘﺴﻧاﻮﺘﻧ ﺎﻬﻨﺗ ﻪﻧ ﺰ

ار

ﯽﻟﻮﻨﻓ ﺐﯿﮐﺮﺗ و هﺪﻨﻨﮐ ﺪﯾﺪﺸﺗ ﻞﻣﺎﻋ ناﻮﻨﻋ ﻪﺑ دﻮﺧ ﻪﮑﻠﺑ ﺪﻨﮐ رﺎﻬﻣ ﺶﻨﮐاو ﻪﻧﮋﯿﺴﮐا بآ ﺮﺑ ﻢﯾﺰﻧآ ﺮﯿﺛﺎﺗ زا ﻞﺻﺎﺣ نﮋﯿﺴﮐا ﺎﺑ لﺎﻌﻓ ار ﮓﻧر ﻞﯿﮑﺸﺗ تﺪﺷ و هداد ﺖﺳا هداد ﺶﯾاﺰﻓا

. ﺞﯾﺎﺘﻧ ﯽﺋاراد

نارﺎﮑﻤﻫ و ﯽﻧﺎﺧ ﻪﻣﺮﮔ )

2010 ( و ﺰﯿﻧ نارﺎﮑﻤﻫ و ﯽﻨﺴﺤﻣ

) 2015 ( ﻫ ﺲﻧﺎﺳا ﺮﯿﺛﺎﺗ توﺎﻔﺗ ﺪﯾﻮﻣ ﺰﯿﻧ ﻨﻣ يور ﻒﻠﺘﺨﻣ يﺎ

ﺎﺑ ﻊ

دﻮﺑ ﻒﻠﺘﺨﻣ ﯽﻤﯾﺰﻧآ )

16 - 15 .(

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

0 100 200 300 400

time (second)

thyme 50 thyme 75

thyme 100 thyme 200

green beans

Fig 2 Effects of different concentrations of thyme essential oil on peroxidase enzymatic activity of

green bean crude extract

3 - 2 - ﻪﻃﻮﻏ ﺎﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﺠﻨﺳ

ﺲﻧﺎﺳا رد تﺎﺠﯾﺰﺒﺳ ندﺮﮐ رو ﻦﺸﯾوآ

ﺮﯿﺛﺎﺗ رد هﺎﯿﺳ بﺮﺗ يرو ﻪﻃﻮﻏ ﺖﻈﻠﻏ

ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ

بﺮﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﻦﺸﯾوآ هﺎﯿﺳ

ﻞﮑﺷ رد )

3 (

نﺎﺸﻧ ﺖﺳا هﺪﺷ هداد .

ﻮﻄﻧﺎﻤﻫ يرو ﻪﻃﻮﻏ دﻮﺷ ﯽﻣ هﺪﻫﺎﺸﻣ ﻪﮐ ر

بﺮﺗ يﺎﻫ ﻪﻧﻮﻤﻧ هﺎﯿﺳ

رد يﺎﻫ ﺖﻈﻠﻏ ﯽﻣﺎﻤﺗ ﻦﺸﯾوآ ﺲﻧﺎﺳا

ﻪﻌﻟﺎﻄﻣ درﻮﻣ

، ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ رد ﯽﻬﺟﻮﺗ ﻞﺑﺎﻗ ﺶﻫﺎﮐ ﻪﺑ ﺮﺠﻨﻣ

بﺮﺗ زاﺪﯿﺴﮐاﺮﭘ هﺎﯿﺳ

ﺖﯿﻟﺎﻌﻓ ﯽﻨﺤﻨﻣ ﻪﮐ ﻪﮑﯾرﻮﻃ ﻪﺑ ﺖﺳا هﺪﺷ

ﺖﻈﻠﻏ رﺎﻬﭼ ﺮﻫ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ 50

، 75 ، 100 و 200

(g/100 ml) ﻦﺸﯾوآ

ﺖﺒﺴﻧ بﺮﺗ يﺎﻫ ﻪﻧﻮﻤﻧ ﻪﺑ هﺎﯿﺳ

نوﺪﺑ هدﻮﺑ ﺮﺘﻤﮐ ﺲﻧﺎﺳا رد يرو ﻪﻃﻮﻏ

ﻪﺘﻓﺎﯾ ﺶﻫﺎﮐ ﺎﻫرادﻮﻤﻧ ﺐﯿﺷ و

ﺖﺳا ﻦﯾاﺮﺑﺎﻨﺑ ﺮﺛا ياراد ﻦﺸﯾوآ ﺲﻧﺎﺳا ﻪﮐ دﻮﻤﻧ نﺎﯿﺑ ناﻮﺗ ﯽﻣ

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ يور ﯽﺒﺳﺎﻨﻣ رﺎﯿﺴﺑ ﯽﮔﺪﻧرادزﺎﺑ ﺪﺷﺎﺑ ﯽﻣ .

0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6

0 100 200 300 400

time (second) Thyme 50 Thyme 75 Thyme 100 Thyme 200

black Spanish radish

Fig 3Effect of dipping in different concentrations of thyme essential oil on peroxidase enzymatic

activity of black Spanish radish

ﻞﮑﺷ رد ﻪﮐ رﻮﻄﻧﺎﻤﻫ )

4 ( ﻪﻧﻮﻤﻧ يرو ﻪﻃﻮﻏ دﻮﺷ ﯽﻣ هﺪﻫﺎﺸﻣ

رد ﺰﺒﺳ ﺎﯿﺑﻮﻟ يﺎﻫ يﺎﻫ ﺖﻈﻠﻏ

50 ، 75 ، 100 ، 200 (g/100

،ﺶﯾوآ ﺲﻧﺎﺳا ml) ﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ

زاﺪﯿﺴﮐا

يﺎﻫ ﻪﻧﻮﻤﻧ ﻪﺑ ﺖﺒﺴﻧ ﺰﺒﺳ ﺎﯿﺑﻮﻟ

ﻪﻃﻮﻏ نوﺪﺑ هﺪﺷ يرو

ﺖﺳا .

ﯽﻣ ﻪﻈﺣﻼﻣ ﻪﮐ رﻮﻄﻧﺎﻤﻫ دﻮﺷ

ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ﺎﺑ ﺲﻧﺎﺳا

ﻦﺸﯾوآ

ﺐﯿﺷ و ﻪﺘﺷاد يدﻮﻌﺻ ﺪﻧور زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﯽﻨﺤﻨﻣ ﯽﻣ و ﺖﺳا ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا رادﻮﻤﻧ دﻮﻤﻧ نﺎﯿﺑ ﻦﯿﻨﭼ ناﻮﺗ

ﺶﯾاﺰﻓا ﻪﮐ

ﺖﻈﻠﻏ ﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﺚﻋﺎﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖ

هﺪﺷ ﺖﺳا .

نﺎﻤﻫ يﺎﻫ ﻞﮑﺷ رد ﻪﮐ رﻮﻃ )

1 ( ، ) 2 ( ، ) 3 ( و ) 4 ( ﺺﺨﺸﻣ

ﮏـﯾ رد زاﺪﯿﺴـﮐاﺮﭘ ﻢﯾﺰـﻧآ ﺖـﯿﻟﺎﻌﻓ ،نﺎﻣز ﺶﯾاﺰﻓا ﺎﺑ ﺖﺳا ﯽﻣ ﺶﯾاﺰﻓا ،ﻦﺸﯾوآ ﺲﻧﺎﺳا زا ﺖﺑﺎﺛ ﺖﻈﻠﻏ ،ﺞﯾﺎـﺘﻧ ﻪـﮐ ﺪﺑﺎﯾ

ﺖﯿﻟﺎﻌﻓ طﺎﺒﺗرا ﻪﺑ طﻮﺑﺮﻣ ﺶﯾاﺰـﻓا ﺮﮕﻧﺎـﯿﺑ ،نﺎـﻣز ﺎـﺑ ﯽﻤﯾﺰﻧآ

هدﻮﺑ اﺮﺘﺴﺑﻮﺳ ﻪﺑ ﻢﯾﺰﻧآ ﯽﺳﺮﺘﺳد ﻪﻣﺮﮔ ﯽﺋاراد ﻖﯿﻘﺤﺗ رد ﻪﮐ

نارﺎــــﮑﻤﻫ و ﯽﻧﺎــــﺧ )

2010 ( و ﯽــــﺧﺮﻔﻬﻗ ﯽﺑﺎﻬــــﺷ ،

نارﺎــــﮑﻤﻫ )

2013 ( نارﺎــــﮑﻤﻫ و ﯽﻨﺴــــﺤﻣ و )

2015 (

ﺖـــــــﺳا هﺪـــــــﺷ ﺖـــــــﺑﺎﺛ ﺰـــــــﯿﻧ )

16 -

14 .(

(7)

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

0 100 200 300 400

time (second)

thyme 50 thyme 75

thyme 100 thyme 200

Green beans

Fig 4 Effect of dipping in different concentrations of thyme essential oil on peroxidase enzymatic

activity of green bean.

ﯽﻧﺎﺷﺎﮐ داﮋﻧ و ﯽﺋاراد (1391) ﺰﯿﻧ

رد ﯽﻘﯿﻘﺤﺗ نﺎﯿﺑ ﺪﻨﺘﺷاد ﻪﮐ

ﺲﻧﺎﺳا ﺎﻫ ﺎﺑ ﺐﯿﮐﺮﺗ نﺪﺷ ﺎﺑ نﮋﯿﺴﮐا ﻂﯿﺤﻣ ﻊﻧﺎﻣ زا هﻮﻬﻗ يا

نﺪﺷ ﯽﻤﯾﺰﻧآ هﺪﺷ و رد ﻪﺠﯿﺘﻧ ﺖﯿﻟﺎﻌﻓ ﻢﯾﺰﻧآ ار زاﺪﯿﺴﮐاﺮﭘ ﺖﺤﺗ

لﺮﺘﻨﮐ ﺪﻨﻫد ﯽﻣ راﺮﻗ ﻪﮐ

ﺎﺑ زا ﯽﺸﺨﺑ ﺞﯾﺎﺘﻧ

ﺗ ﻖﯿﻘﺤ ﺮﺿﺎﺣ

ﺖﻘﺑﺎﻄﻣ ﺖﺷاد ] 17 .[

ﺞﯾﺎﺘﻧ تﺎﻘﯿﻘﺤﺗ ﺮﯾﺎﺳ ﻦﯿﻘﻘﺤﻣ ﺰﯿﻧ طﺎﺒﺗرا

ﺖﯿﻟﺎﻌﻓ ﯽﺘﻧآ ﺲﻧﺎﺳا ﯽﻧاﺪﯿﺴﮐا يﺎﻫ

ﯽﻫﺎﯿﮔ و ﺖﻌﻧﺎﻤﻣ ﯽﮔﺪﻨﻨﮐ

ﺖﯿﻟﺎﻌﻓ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ رد

تﺎﺠﯾﺰﺒﺳ ﻒﻠﺘﺨﻣ

ﺖﺤﺗ ﺮﯿﺛﺄﺗ

ﺲﻧﺎﺳا يﺎﻫ ار ﯽﻫﺎﯿﮔ نﺎﺸﻧ

ﯽﻣ ﺪﻫد ﻪﮐ ﺪﯾﻮﻣ ﺞﯾﺎﺘﻧ ﻖﯿﻘﺤﺗ

ﺮﺿﺎﺣ ﯽﻣ ﺷﺎﺑ ﻨ ﺪ.

ﻪﻣﺮﮔ ﯽﺋاراد ﯽﻧﺎﺧ

و ﻤﻫ نارﺎﮑ (2010)

،

ﯽﺑﺎﻬﺷ ﯽﺧﺮﻔﻬﻗ و نارﺎﮑﻤﻫ ) 2013 ( ﺮﯿﺛﺎﺗ ﺲﻧﺎﺳا يﺎﻫ

،ﮏﺨﯿﻣ

ﻪﻧﺎﯾزار و هﺮﯾز رد ﺮﯿﻏ لﺎﻌﻓ يزﺎﺳ ﻢﯾﺰﻧآ زاﺪﯿﺴﮐاﺮﭘ تﺎﺠﯾﺰﺒﺳ

ﻒﻠﺘﺨﻣ )

،ﻮﻫﺎﮐ

،ﺪﯿﻔﺳ ﻢﻠﮐ ﻢﻠﮐ

،ﺰﻣﺮﻗ وﺪﮐ ﯽﺘﺷرﻮﺧ و ﺐﯿﺳ

ﯽﻨﯿﻣز ( ار شراﺰﮔ ﺪﻧدﻮﻤﻧ ] 14 و 16 .[

ﺶﯾاﺰﻓا ﺎﺑ نﺎﻣز تﺪﻣ

،ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺖﯿﻟﺎﻌﻓ خﺮﻧ و تﺪﺷ

ﯽﻤﯾﺰﻧآ ) بﺬﺟ ( ﺶﻫﺎﮐ

ﯽﻣ ﺪﺑﺎﯾ ؛ ﻪﮐ ﯽﻣ ﻢﮐ ﺖﻠﻋ ﻪﺑ ﺪﻧاﻮﺗ سﺮﺘﺳد رد ياﺮﺘﺴﺑﻮﺳ نﺪﺷ

ﻢﮐ و ﻢﯾﺰﻧآ ﺮﮕﯾد يﻮﺳ زا ؛ﺪﺷﺎﺑ ﻢﯾﺰﻧآ ﻮﯿﺗاﺪﯿﺴﮐا ﺖﯿﻟﺎﻌﻓ نﺪﺷ

ﺖﻌﻧﺎﻤﻣ تﺎﺒﯿﮐﺮﺗ ﻞﯿﮑﺸﺗ ﯽﻣ ﺰﯿﻧ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ زا هﺪﻨﻨﮐ

رد ﺪﻧاﻮﺗ

درﻮﻣ ﻦﯾا ﺷﺎﺑ ﺮﺛﻮﻣ

ﺪ ] 14 و 16 .[

ﺎﺑ نﺪﺷ ﺐﯿﮐﺮﺗ ﺎﺑ ﺎﻫ ﺲﻧﺎﺳا

ﻣ ﻂﯿﺤﻣ نﮋﯿﺴﮐا رد و هﺪﺷ ﯽﻤﯾﺰﻧآ نﺪﺷ يا هﻮﻬﻗ ﺶﻨﮐاو ﻊﻧﺎ

ﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﺠﯿﺘﻧ ﺐﻟﺎﻄﻣ ﺎﺑ ﻪﮐ ﺪﻨﻨﮐ ﯽﻣ لﺮﺘﻨﮐ ار زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰ

ﺪﺷﺎﺑ ﯽﻣ ﻖﺒﻄﻨﻣ ﺶﻫوﮋﭘ ﻦﯾا ]

17 .[

ﯽﺋاراد ﯽﻧﺎﺧ ﻪﻣﺮﮔ و

نارﺎﮑﻤﻫ (2010) ﺪﻨﺘﺷاد نﺎﯿﺑ

ﻪﮐ رد ﺲﻧﺎﺳا

،ﻪﻧﺎﯾزار ﺶﯾاﺰﻓا

نﺎﻣز ﺖﯿﻟﺎﻌﻓ و ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ﺲﻧﺎﺳا

ﺮﺠﻨﻣ ﻪﺑ ﺶﯾاﺰﻓا ﺪﯾﺪﺷ ﻟﺎﻌﻓ

ﺖﯿ ﻢﯾﺰﻧآ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﻠﮐ

ﺪﯿﻔﺳ و ﻢﻠﮐ ﺰﻣﺮﻗ ﺪﺷ ﯽﻟﺎﺣرد

ﻪﮐ رد ﺲﻧﺎﺳا ﺲﮑﻋ ﮏﺨﯿﻣ ﻦﯾا

ﺖﻟﺎﺣ هﺪﻫﺎﺸﻣ ﺪﯾدﺮﮔ و

ﺶﯾاﺰﻓا ﺖﻈﻠﻏ ﺲﻧﺎﺳا ﮏﺨﯿﻣ ﺮﺛا ﯽﮔﺪﻧرادزﺎﺑ يﺮﺗﻻﺎﺑ

ﺖﺷاد .

ﺎﻬﻧآ يﺮﯿﮔ ﻪﺠﯿﺘﻧ ﺪﻧدﺮﮐ

ترﺪﻗ ﻪﮐ ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ و

رﺎﻬﻣ ﯽﮔﺪﻨﻨﮐ

نﮋﯿﺴﮐا

، ﺎﺳا ﺲﻧ ﮏﺨﯿﻣ ﺮﺗﻻﺎﺑ ﺐﺗاﺮﻣ ﻪﺑ زا

ﺲﻧﺎﺳا ﺎﯾزار ﻪﻧ ﺖﺳا

] 16 .[

ﺰﯿﻧ ﺶﻫوﮋﭘ ﻦﯾا رد ﻦﺸﯾوآ ﺲﻧﺎﺳا

بﺮﺗ رد هﺎﯿﺳ بﻮﺧ

ﺶﻫﺎﮐ ﺐﺟﻮﻣ و هدﺮﮐ ﻞﻤﻋ ﺖﯿﻟﺎﻌﻓ

ﻢﯾﺰﻧآ زاﺪﯿﺴﮐاﺮﭘ ﺪﺷ

ﻪﮐ

ﺮﮕﻧﺎﯿﺑ ياﺮﺘﺴﺑﻮﺳ ﺶﻫﺎﮐ ﯽﻓﺮﺼﻣ

و ﺎﯾ ﺶﻫﺎﮐ نﮋﯿﺴﮐا درﻮﻣ زﺎﯿﻧ

ﻢﯾﺰﻧآ دﺎﺠﯾا ياﺮﺑ ﮓﻧر

هﻮﻬﻗ يا ﯽﻣ ﺪﺷﺎﺑ ﻪﮐ نﺎﺸﻧ هﺪﻨﻫد ﯽﯾﺎﻧاﻮﺗ

رد ﻦﺸﯾوآ ﺲﻧﺎﺳا ﺶﻨﮐاو

ﺎﺑ نﮋﯿﺴﮐا ﯿﺤﻣ ﻂ و ﺖﻌﻧﺎﻤﻣ زا ﺶﻨﮐاو

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ و

ﯽﻠﭘ لﻮﻨﻓ زاﺪﯿﺴﮐا ) هﻮﻬﻗ يا نﺪﺷ ﯽﻤﯾﺰﻧآ (

ﯽﻣ ﺪﺷﺎﺑ . ﺲﻧﺎﺳا ﺎﻫ ﺎﺑ ﺐﯿﮐﺮﺗ نﺪﺷ ﺎﺑ نﮋﯿﺴﮐا ﻂﯿﺤﻣ ﻊﻧﺎﻣ

هﻮﻬﻗ ﺶﻨﮐاو نﺪﺷ يا

ﯽﻤﯾﺰﻧآ هﺪﺷ و رد ﻪﺠﯿﺘﻧ ﺖﯿﻟﺎﻌﻓ ﻢﯾﺰﻧآ

زاﺪﯿﺴﮐاﺮﭘ ار

لﺮﺘﻨﮐ ﯽﻣ ﺪﻨﻨﮐ . ﺞﯾﺎﺘﻧ ﯽﺋاراد ﯽﻧﺎﺧ ﻪﻣﺮﮔ و

نارﺎﮑﻤﻫ ) 2010 ( ﺮﮕﻧﺎﯿﺑ ﺗ ﺮﯿﺛﺄ ﺖﺒﺜﻣ ﺲﻧﺎﺳا ﮏﺨﯿﻣ رد ﺖﻌﻧﺎﻤﻣ

زا ﺖﯿﻟﺎﻌﻓ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ رد

تﺎﺠﯾﺰﺒﺳ درﻮﻣ

ﻪﻌﻟﺎﻄﻣ ) ﻢﻠﮐ

ﺪﯿﻔﺳ و ﻢﻠﮐ ﺰﻣﺮﻗ ( دﻮﺑ و ﺮﺛا ﺖﻌﻧﺎﻤﻣ ﯽﮔﺪﻨﻨﮐ ﺲﻧﺎﺳا ﺎﺑ ﺶﯾاﺰﻓا

ﺖﻈﻠﻏ ﺪﯾﺪﺸﺗ ﺲﻧﺎﺳا ﯽﻣ

ﺪﺷ ] 16 .[

رد ﺰﺒﺳ ﺎﯿﺑﻮﻟ ﺎﺑ ﺶﯾاﺰﻓا

ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﯽﻤﯾﺰﻧآ

، تﺪﺷ و خﺮﻧ ﺖﯿﻟﺎﻌﻓ ﯽﻤﯾﺰﻧآ ) بﺬﺟ (

ﯽﻣ ﺶﯾاﺰﻓا ﯾ

ﺪﺑﺎ ﻪﮐ ﻦﯾا ﺶﯾاﺰﻓا ﯽﻣ ﺪﻧاﻮﺗ ﻪﺑ ﺖﻠﻋ ﺶﯾاﺰﻓا

ياﺮﺘﺴﺑﻮﺳ سﺮﺘﺳد رد

ﻢﯾﺰﻧآ و ﻢﮐ نﺪﺷ ﺮﺛا ﯽﮔﺪﻧرادزﺎﺑ ﻢﯾﺰﻧآ

رد ﺖﻈﻠﻏ يﺎﻫ توﺎﻔﺘﻣ ﺲﻧﺎﺳا ﺪﺷﺎﺑ ﻦﺸﯾوآ ]

14 و 16 .[

4 - يﺮﯿﮔ ﻪﺠﯿﺘﻧ

زا تﻻﻮﺼﺤﻣ ﯽﻧﺎﻣرﺎﺒﻧا ﻆﻔﺣ ﺖﻬﺟ ﯽﻧﻮﮔﺎﻧﻮﮔ يﺎﻫ شور

ﺒﻃ يﺎﻫ ﺲﻧﺎﺳا ﻪﮑﻨﯾا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ و ددﺮﮔ ﯽﻣ هدﺎﻔﺘﺳا ﯽﯾاﺬﻏ ﯽﻌﯿ

ﯽﺘﻧآ ﺖﯿﺻﻮﺼﺧ ياراد ﯽﺑوﺮﮑﯿﻣ ﺪﺿ ﺖﯿﺻﺎﺧ ﺮﺑ هوﻼﻋ ﻦﯾﺰﮕﯾﺎﺟ ﺖﻬﺟ نآ زا ناﻮﺗ ﯽﻣ ﺲﭘ ﺪﻨﺷﺎﺑ ﯽﻣ ﺰﯿﻧ ﯽﻧاﺪﯿﺴﮐا دﻮﻤﻧ هدﺎﻔﺘﺳا لﻮﻤﻌﻣ يﺎﻫ شور ندﻮﻤﻧ .

ﻢﯾﺰﻧآ ندﻮﻤﻧ لﺎﻌﻓ ﺮﯿﻏ

ﺶﻫﺎﮐ و ﮓﻧر ﺖﻓا ﻞﯿﺒﻗ زا ﯽﺗﻼﮑﺸﻣ تراﺮﺣ ﻂﺳﻮﺗ زاﺪﯿﺴﮐاﺮﭘ ﺲﻧﺎﺳا زا هدﺎﻔﺘﺳا رد ﻪﮐ دراد هاﺮﻤﻫ ﻪﺑ ار يﺬﻐﻣ داﻮﻣ ﻞﯿﺒﻗ ﻦﯾا

ﺶﻫﺎﮐ ﺐﺟﻮﻣ لﺎﺣ ﻦﯿﻋ رد و هدﻮﺒﻧ حﺮﻄﻣ ﻞﺋﺎﺴﻣ ﺖﯿﻟﺎﻌﻓ

ﻢﯾﺰﻧآ

ددﺮﮔ ﯽﻣ ﺰﯿﻧ زاﺪﯿﺴﮐاﺮﭘ .

ﻪﮐ داد نﺎﺸﻧ ﻖﯿﻘﺤﺗ ﻦﯾا ﺞﯾﺎﺘﻧ ناﻮﺗ ﯽﻣ

زا ﯽﻌﯿﺒﻃ يﺎﻫ ﺲﻧﺎﺳا رد

زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ

دﻮﻤﻧ هدﺎﻔﺘﺳا تﺎﺠﯾﺰﺒﺳ رد زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﻪﮑﻨﯾا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ،

ﻦﯾا رد ﺪﺷﺎﺑ ﯽﻣ توﺎﻔﺘﻣ ﻒﻠﺘﺨﻣ ﻪﮐ ﺪﺷ ﺺﺨﺸﻣ ﻪﻌﻟﺎﻄﻣ

دﺮﮑﻠﻤﻋ ﺶﻫﺎﮐ ﺖﻬﺟ رد ﯽﺑﻮﺧ ﺮﺛا ﺪﻧاﻮﺗ ﯽﻣ ﻦﺸﯾوآ ﺲﻧﺎﺳا

(8)

بﺮﺗ زاﺪﯿﺴﮐاﺮﭘ هﺎﯿﺳ

ﺐﺟﻮﻣ ﺰﺒﺳ ﺎﯿﺑﻮﻟ رد ﺎﻣا ﺪﺷﺎﺑ ﻪﺘﺷاد

ﯽﻣ نآ دﺮﮑﻠﻤﻋ ﺶﯾاﺰﻓا ددﺮﮔ

. ﺖﯿﻠﺑﺎﻗ ﻪﻌﻟﺎﻄﻣ ﻦﯾا عﻮﻤﺠﻣ رد

ﯽﺗراﺮﺣ ﺮﯿﻏ يزﺎﺳ لﺎﻌﻓﺮﯿﻏ رد ﯽﻫﺎﯿﮔ يﺎﻫ ﺲﻧﺎﺳا زا هدﺎﻔﺘﺳا ا داد نﺎﺸﻧ ار زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﻢﯾﺰﻧآ ﻊﺒﻨﻣ عﻮﻧ ﺐﺴﺣﺮﺑ ﯽﺘﺴﯾﺎﺑ ﺎﻣ

دﻮﻤﻧ هدﺎﻔﺘﺳا ﺐﺳﺎﻨﻣ ﺲﻧﺎﺳا زا .

5 - ﻊﺑﺎﻨﻣ

[1] Rahemi, M. 2004. Post harvest physiology, an introduction to fruits and vegetables physiology and handling. Shiraz University press (in Persian).

[2] Holley, R. A., Patel, D. 2005. Improvement in shelf- life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiology, 22, 273- 292.

[3] Maskoki, A M. 1998. The technology of essential oils and medicinal products production from medical herbs. Reported research at Iranian Research Organization for Science and Technology, Mashhad Unit (in Persian).

[4] Boyraz, N, Ozcan, M. 2005. Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savory (Satureja hortensis L.) growing wild in Turkey. International Journal of Food Microbiology, 1, 1-5.

[5] Lanciotti, R, Gianotti, A, Patrignani, F, Belletti, N, Gverzoni, M E, Gardini, F. 2004.

Use of natural aroma compound to improve shelf life and safety of minimally processed fruit. Trends in Food Science and Technology, 15, 201-208.

[6] Omidbigi, R, 2009. Medicinal plant production and processing. Astan Ghodse Razavi publisher, 5th ed, pp: 653 (Persian).

[7] Singh, A, Singh, RH, Bhunia, AK, singh, N. 2003. Efficacy of plant essential oils as antimicrobial agents against Listeria monocytogenes in hotdogs. LWT- Food Science and Technology, 36, 787-94.

[8] Tassou, CC, Koutsou Manis, K, Nychas, G.

JE. 2000. Inhibition of salmonella entertidis

& staphylococcus aureus in nutrient broth by mint essential oil, Food Research International, 33, 273-280.

[9] Lambert, RJW, Skandamis, PN, Coote, PJ, Nychas, GJE. 2001. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and

carvacrol. Journal of Applied Microbiology., 91, 453-462.

[10] Ultee, A, Kets, PE, Smid, EJ. 1999.

Mechanism of action of carvacol on the foodborne pathogen bacillus cereus. Applied

& Environmental Microbiology, 65, 4606- 4610.

[11] Nikos Gand Tzortzakis, A. 2007.

Maintaining postharvest quality of fresh produce with volatile compounds. Innovative food science & emerging technologies, 8, 11- 116

[12] Ercan, S. S, Soysal, C. 2011. Effect of ultrasound and temperature on tomato peroxidase. Ultrasonics Sonochemistry, 18, 689-695.

[13] Ponec, AG, Del Valle, CE, Roura, SI.

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Study of the effect of thyme essential oil on the reduction of peroxidase enzyme activity in the black Spanish radish and green

bean

Mohseni, N. M. ^1, Daraei Garmakhany, A.², Mohamadi Sani, A.³

1. Young Researchers and Elite Club, Sari Branch, Islamic Azad University, Sari, Iran 2. Department of Food Science and Technology, Toyserkan Faculty of agricultural science and natural

resources, Bu-Ali Sina University, Hamadan, Iran

3. Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran.

(Received: 2017/09/21 Accepted:2018/02/04)

Enzymes in plant tissues can have undesirable or desirable effects on the quality of fruits and vegetables; therefore management of their change in fruits and vegetables is one of the concerns in food industry. Nowadays consumer's trend has been oriented to use fresh products or foods prepared by little process, therefore producers are interested the ways to increase shelf life by use of alternative methods. The aim of this work was to study the ability of thyme (Thymus vulgaris L.) essential oil in reduction of peroxidase enzyme activity (as an important biochemical degradation factors) in the black Spanish radish and green bean. For this purpose, plant extract containing peroxidase enzyme was mixed with a substrate mixture composed from guaiacol, hydrogen peroxide, sodium phosphate buffer and different concentrations of thyme essential oil (50, 75, 100 and 200 μg/100 ml). The absorbance of the samples was measured at 470 nm for 400 seconds spectrophotometrically. Results of this study showed that the peroxidase enzyme activity in the black Spanish radish and green beans were 4625 and 51 (gram of vegetable × second / unit of activity) respectively and by addition of thyme essential oil in the above concentrations the peroxidase enzyme activity of black Spanish radish were 2655, 2550, 2015 and 2162 (gram of vegetable × second / unit of activity) respectively and the peroxidase enzyme activity of green bean were 708, 6/551, 579 and 690 (gram of vegetable × second / unit of activity) respectively.

Keywords: Black Spanish radish, Green beans, Thyme essential oil, Peroxidase enzyme.

 Corresponding Author E-Mail Address: [email protected]