ﺰﺒﺳ وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﯽﺗراﺮﺣ ﺮﯿﻏ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ :
لﺪﻣ ﺗ ﺦﺳﺎﭘ ﺢﻄﺳ يزﺎﺳ ﺲﻧﺎﺳا ﺮﯿﺛﺄ
،هﺮﯾز يﺎﻫ
ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار
ﯽﻧﺎﺟﺎﻗآ ﺲﺟﺮﻧ ﯽﻧﺎﺧ ﻪﻣﺮﮔ ﯽﺋاراد ﺮﯿﻣا ،1
2
*
1 - ﯽﺳﺪﻨﻬﻣ هوﺮﮔ ،رﺎﯾدﺎﺘﺳا ناﺮﯾا ،ناﺪﻤﻫ ،ﺎﻨﯿﺳ ﯽﻠﻋﻮﺑ هﺎﮕﺸﻧاد ،رﺎﻬﺑ ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ
.
2 - ﯽﺳﺪﻨﻬﻣ هوﺮﮔ ،رﺎﯾدﺎﺘﺳا ﺗ ﯽﻌﯿﺒﻃ ﻊﺑﺎﻨﻣ و ﯽﻨﻓ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ
ناﺮﯾا ،ناﺪﻤﻫ ،ﺎﻨﯿﺳ ﯽﻠﻋﻮﺑ هﺎﮕﺸﻧاد ،نﺎﮐﺮﺴﯾﻮ .
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 14 / 08 / 99
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 09 / 12 / 99
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
،ﺰﺒﺳ وﺪﮐ
،زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ
،هﺮﯾز ﺲﻧﺎﺳا
،ﮏﺨﯿﻣ ﺲﻧﺎﺳا
ﻪﻧﺎﯾزار ﺲﻧﺎﺳا .
DOI: 10.52547/fsct.18.05.09
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ :
شزرا ﺶﻫﺎﮐ رد ﯽﺗراﺮﺣ يرواﺮﻓ ﺮﯿﺛﺄﺗ و ﯽﯾﺎﯿﻤﯿﺷ داﻮﻣ تاﺮﻀﻣ ﻪﺑ نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ ﯽﻫﺎﮔآ ﺶﯾاﺰﻓا ﺎﺑ ﻪﯾﺬﻐﺗ ﺎﻘﺗ ،يا ﺖﺳا ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا هﺪﺷ يروآﺮﻓ ﺮﺘﻤﮐ ﺎﯾ و هزﺎﺗ ﯽﯾاﺬﻏ داﻮﻣ زا هدﺎﻔﺘﺳا و ﺪﯿﻟﻮﺗ ياﺮﺑ يﺎﺿ .
رد
ﺲﻧﺎﺳا ﯽﯾﺎﻧاﻮﺗ ﻪﻌﻟﺎﻄﻣ ﻦﯾا ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،ﺰﺒﺳ هﺮﯾز يﺎﻫ
) ﺖﻈﻠﻏ يﺎﻫ 50 ، 75 ، 100 ، 200 ppm مﺮﻓ و
ﺺﻟﺎﺧ ﺪﺷ ﯽﺳرﺮﺑ ﺰﺒﺳ وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ رد ( .
ﺎﻬﻨﺗ ﻪﮐ داد نﺎﺸﻧ ﺞﯾﺎﺘﻧ و ﺺﻟﺎﺧ مﺮﻓ
ﺖﻈﻠﻏ 200ppm
ﺖﻈﻠﻏ و ﺺﻟﺎﺧ مﺮﻓ ﻦﯿﻨﭽﻤﻫ و ﺰﺒﺳ هﺮﯾز ﺲﻧﺎﺳا يﻻﺎﺑ يﺎﻫ
) 100 و 200ppm ( ﺲﻧﺎﺳا
ﺖﻈﻠﻏ زا هدﺎﻔﺘﺳا ﺎﻣا ﺪﻧدﻮﺑ اراد ار ﺰﺒﺳ وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﯽﯾﺎﻧاﻮﺗ ،ﻪﻧﺎﯾزار يﺎﻫ
50 و
75 ppm هﺪﺷ ﺰﯿﻧ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﺚﻋﺎﺑ ﻪﻧﺎﯾزار ﺲﻧﺎﺳا دﻮﺑ
. ﻪﯿﻠﮐ رد ﮏﺨﯿﻣ ﺲﻧﺎﺳا
ﺖﻈﻠﻏ ﺪﺷ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ ﻪﮐ
نﺎﺸﻧ هﺪﻨﻫد ﯽﯾﺎﻧاﻮﺗ ﮏﺨﯿﻣ ﺲﻧﺎﺳا
رد ﺶﻨﮐاو ﺎﺑ نﮋﯿﺴﮐا ﻂﯿﺤﻣ و ﺖﻌﻧﺎﻤﻣ زا ﺶﻨﮐاو زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ و
ﯽﻠﭘ لﻮﻨﻓ زاﺪﯿﺴﮐا ) هﻮﻬﻗ يا نﺪﺷ
ﯽﻤﯾﺰﻧآ ( ﯽﻣ ﺪﺷﺎﺑ . ﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ياﺮﺑ ﻂﯾاﺮﺷ ﻦﯾﺮﺘﻬﺑ ﺖﻈﻠﻏ ﻞﻣﺎﺷ هﺮﯾز ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﺖﺤﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰ
ﺲﻧﺎﺳا ppm 5 / 194 ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و 02
/0 ﻪﺑ ﯽﺑﺎﯾ ﺖﺳد ياﺮﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻪﮑﯿﻟﺎﺣ رد دﻮﺑ ﻪﯿﻧﺎﺛ
ﻢﮐ زا هدﺎﻔﺘﺳا ﻞﻣﺎﺷ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻦﯾﺮﺗ 200ppm
ﻪﻧﺎﯾزار ﺲﻧﺎﺳا ﺮﻔﺻ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و
و ،ﻪﯿﻧﺎﺛ 200ppm
ﯿﻣ ﺲﻧﺎﺳا ﮏﺨ
ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و 7
/1 ﯽﻣ ﻪﯿﻧﺎﺛ ﺪﺷﺎﺑ .
ﻪﻠﺠﻣ مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯽﯾ اﯾ ناﺮ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ
ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ
1 - ﻪﻣﺪﻘﻣ
ﻢﯾﺰﻧآ ﺎﻫ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ ﺎﺑ ﺶﻘﻧ يﺎﻫ ﺑ ﯿ ﻮﻟﻮ ژﯾ ﮑ ﯽ ﻪﮐ ﺪﻨﺘﺴﻫ ﻒﻠﺘﺨﻣ
ﺴﺑ ترﻮﺻ ﻪﺑ ﯿ
ﺼﺼﺨﺗ رﺎ ﯽ
ﺶﻨﮐاو يﺎﻫ ﺑ ﯿ ﻮﻟﻮ ژﯾ ﮑ ﯽ ﻪﺑ طﻮﺑﺮﻣ
ﺴﺑ ﯿ رﺎ ي ا آﺮﻓ ز ﯾ ﺎﻫﺪﻨ ي ﻟﻮﺑﺎﺘﻣ ﯿ هﺪﻧز تادﻮﺟﻮﻣ ﮏ ار
ﻟﺎﺗﺎﮐ ﯿ ﺰ
ﯽﻣ ﺪﻨﻨﮐ ] 1 [ . ﻢﯾﺰﻧآ ﺎﻫ ﺶﻨﮐاو يﺎﻫ ﺷ ﯿﻤ ﯿﺎ ﯽﯾ ﺮﻃ زا ار ﯾ ﺪﺒﺗ ﻖ ﯾ ﻞ
ﻟﺎﺗﺎﮐ لﻮﺼﺤﻣ ﻪﺑ اﺮﺘﺴﺑﻮﺳ ﯿ
ﺰ ﯾ ﺮﺴﺗ ﺎ ﯾ ﻊ هدﺮﮐ زا و ﯾ ﮏ ﯾ داﺪﻌﺗ ﺎ
ﺑﯿ ﺮﺘﺸ ي ا ﻠﭘ ﺖﻠﮑﺳ ﯽ ﺘﭘ ﯿ ﺪ ي زا ﻞﮑﺸﺘﻣ ﻟاﻮﺗ
ﯽ ﺳا ﯿ ﺎﻫﺪ ي ﻣآ ﯿ ﺎﺑ ﻪﻨ
ﭘرﺎﻣ رﺎﺘﺧﺎﺳ ﯿ
ﭽ ﯽ ﮑﺸﺗ ﯿ ﻞ هﺪﺷ ﺪﻧا . ﻟﺎﻌﻓ ﯿ ﺖ ﻢﯾﺰﻧآ ﺎﻫ
ًﻻﻮﻤﻌﻣ ﻪﺑ
ﺪﻌﺑ ﻪﺳ رﺎﺘﺧﺎﺳ ي
نآ ﺎﻫ ﺖﺳا ﻪﺘﺴﺑاو .
ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ رد
ًﻻﻮﻤﻌﻣ
ﻟﺎﻌﻓ ﯿ ﺖ ﻢﯾﺰﻧآ يﺎﻫ بﻮﻠﻄﻣ بﺮﺨﻣ ﻧ
و هدﻮﺒ ﮐ ﺖﻓا ﺚﻋﺎﺑ ﯿﻔ
ﯿ رد ﺖ
ﻃ ﯽ رادرﺎﺒﻧا هرود ي
ﯽﻣ ﺪﻧﻮﺷ . لﻮﻨﻓ ﯽﻠﭘ ﻢﯾﺰﻧآ زاﺪﯿﺴﮐا
) (PPO
ﯾ ﮑ ﯽ زا ﻢﯾﺰﻧآ يﺎﻫ ﺎﻨﺻ رد بﺮﺨﻣ ﯾ
اﺬﻏ ﻊ ﯽﯾ ﺖﺳا ﻪﮐ
ﺌﺗوﺮﭘﻮﻟﺎﺘﻣ ﯿﻨ ﯽ ﺴﮐا ﯿ زﺎﺘﮐدوروﺪ و
وﺎﺣ ي ﺖﺳا ﺲﻣ ﻪﮐ
لﻮﺌﺴﻣ
ﺶﻨﮐاو يﺎﻫ هﻮﻬﻗ يا رد نﺪﺷ هﻮﯿﻣ
ﺎﻫ ﺰﺒﺳ و ﯾ تﺎﺠ ) ﺳ ﯿ ،ﺐ
ﺑﻼﮔ ﯽ و چرﺎﻗ ،ﻮﻫﺎﮐ ،ﻮﻠﻫ ، (....
ﺧﺮﺑ و ﯽ ﺎﻫاﺬﻏ ي رد ﯾﺎ ﯽﯾ
ﯽﻣ ﺪﺷﺎﺑ ] 2[
. اﺮﺑ ي ﻟﺎﻌﻓ لﺮﺘﻨﮐ ﯿ
ﺖ زا PPO ﮐﺮﺗ ﯿ تﺎﺒ ﺪﺿ
هﻮﻬﻗ يا ﻧ نﺪﺷ ﯿﻈ ﻔﻟﻮﺳ ﺮ ﯿ ﺪﺳ روﺮﻠﮐ ،ﺖ ﯾ
ﺳ ،ﻢ ﯿ ﺌﺘﺴ ﯿ و ،ﻦ ﯾ ﻣﺎﺘ ﯿ ﻦ
ﺳ و ث ﯿ ﻣﺎﻨ ﯿ ﺳا ﮏ ﯿ هدﺎﻔﺘﺳا ﺪ ﯽﻣ
دﻮﺷ ا زا ماﺪﮐﺮﻫ ﻪﮐ ﯾ
ﮐﺮﺗ ﻦ ﯿ تﺎﺒ
ﻧﺎﮑﻣ ﯿ ﮔﺪﻧرادزﺎﺑ ﻢﺴ ﯽ
ﻟﺎﻌﻓ ﯿ ﺰﻧآ ﺖ ﯾ ﻢ صﻮﺼﺨﻣ و توﺎﻔﺘﻣ PPO
ﺪﻧراد ار دﻮﺧ ﻪﺑ ]
3 [ .
اﺬﻏ تﻻﻮﺼﺤﻣ نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ ﯽﯾ
رﺎﻋ ي زا ﻪﮕﻧ هﺪﻧراد ﺎﻫ ار
ﺟﺮﺗ ﯿ ﺢ ﯽﻣ ﺪﻨﻫد ﺎﻨﺑ اﺮﺑ ﯾ ﺎﻤﺗ ﻦ ﯾ ﻧﺎﻬﺟ ﻞ ﯽ ﺶﻫﺎﮐ ﺖﻤﺳ ﻪﺑ ﻣ
فﺮﺼ
ﯽﻧدوﺰﻓا يﺎﻫ ﺷ ﯿﻤ ﯿﺎ ﯽﯾ ﻪﮐ ﺖﺳا ﯽﻣ
ﺪﻨﻧاﻮﺗ ﻔﻨﻣ تاﺮﺛا ﯽ
ور ي
ﺪﻧراﺬﮕﺑ هﺪﻨﻨﮐ فﺮﺼﻣ ﺖﻣﻼﺳ .
يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ﻢﯾﺰﻧآ
ﺎﻫ
ًﻻﻮﻤﻌﻣ
ﻂﯾاﺮﺷ ﺮﯿﯿﻐﺗ و فﺬﺣ ،اﺮﺘﺴﺑﻮﺳ ﻪﺑ ﯽﺳﺮﺘﺳد ﺖﻌﻧﺎﻤﻣ ﻖﯾﺮﻃ زا ﺮﻈﻧ زا ﻂﯿﺤﻣ ﺐﯾﺮﺨﺗ ﺎﯾ و نﮋﯿﺴﮐا دﻮﺟو مﺪﻋ ﺎﯾ دﻮﺟو و pH
ﺗوﺮﭘ ﺶﺨﺑ ﻢﯾﺰﻧآ ﯽﻨﯿﺌ
) نﻮﯿﺳارﻮﺗﺎﻧد (
ترﻮﺻ ﯽﻣ دﺮﯿﮔ . آﺮﻓ ﯾ ﺪﻨ
ﺗراﺮﺣ ﯽ هدﺎﺳ ﻦﯾﺮﺗ اﺮﺑ شور ي لﺮﺘﻨﮐ هﻮﻬﻗ يا نﺪﺷ هﻮﯿﻣ ﺎﻫ و
ﺰﺒﺳ ﯾ ﭽﻧﻼﺑ نآ ﻪﺑ ﺖﻌﻨﺻ رد ﻪﮐ ﺖﺳا تﺎﺠ ﯿ
و ﮓﻨ
رﻮﺘﺳﺎﭘ ﯾ ﺳاﺰ ﯿ نﻮ ﯽﻣ ﺪﻨﯾﻮﮔ . تراﺮﺣ زا هدﺎﻔﺘﺳا ﺎﻣا ﯽﻣ
ﺪﻧاﻮﺗ تاﺮﺛا
ﻔﻨﻣ ﯽ ور ي لﻮﺼﺤﻣ ﻢﻌﻃ و ﺮﻄﻋ ﻪﺘﺷاﺬﮔ
ﺧﺮﺑ و ﯽ ﺬﻐﻣ داﻮﻣ ي
و ﻞﺜﻣ ﯾﺘ ﻣﺎ ﯿ ﻠﭘ ،ث ﻦ ﯽ لﻮﻨﻓ ﺎﺳ و ﺎﻫ ﯾ ﮐﺮﺗ ﺮ ﯿ ﺘﻧآ تﺎﺒ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ار
ﺮﺨﺗ ﯾ ﺎﻤﻧ ﺐ ﯾ ﺪ ]6 - 4 [.
درﻮﻣ ﺞﯾار ﯽﺗراﺮﺣ رﺎﻤﯿﺗ ﺶﯿﭘ ﮓﻨﯿﭽﻧﻼﺑ
رد هدﺎﻔﺘﺳا هﻮﯿﻣ
ﺎﻫ تﺎﺠﯾﺰﺒﺳ و يﺎﻫﺪﻨﯾآﺮﻓ و يروآﺮﻓ زا ﻞﺒﻗ
ﺖﺳا يزﺎﺳوﺮﺴﻨﮐ و ندﺮﮐ ﮏﺸﺧ ،دﺎﻤﺠﻧا .
ﭽﻧﻼﺑ رد ﮓﻨﯿ بآ
غاد ) 100 - 85 ﻪﺟرد ﯽﺘﻧﺎﺳ داﺮﮔ ( ﻪﺑ ﺮﺠﻨﻣ ًﻻﻮﻤﻌﻣ ﺖﺳد زا
و ﯽﻧﺪﻌﻣ داﻮﻣ ﺪﻨﻧﺎﻣ يﺬﻐﻣ داﻮﻣ نداد ﻦﯿﻣﺎﺘﯾو
ﺎﻫ ﯽﻣ دﻮﺷ ] 7 .[
ﮓﻨﯿﭽﻧﻼﺑ ياﺮﺑ هدﺎﻔﺘﺳا درﻮﻣ ﯽﻠﮐ ﺺﺧﺎﺷ
هﻮﯿﻣ ﺎﻫ ،تﺎﺠﯾﺰﺒﺳ و
لﺎﻌﻓ ﺮﯿﻏ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ يزﺎﺳ )
EC1.11.1.7 (
ﻪﮐ ﺖﺳا
موﺎﻘﻣ ﻦﯾﺮﺗ ﺖﺳا تراﺮﺣ ﺮﺑاﺮﺑ رد ﻢﯾﺰﻧآ .
ﯽﻫﺎﯿﮔ زاﺪﯿﺴﮐاﺮﭘ
) (POD ﻪﺑ ﻪﮐ ﺖﺳا ﯽﻤﯾﺰﻧآ مﺮﻓ
يﺎﻫ ﺨﻣ ﺖﻓﺎﯾ نﺎﻫﺎﯿﮔ رد ﻒﻠﺘ
ياراد و هﺪﺷ ﺶﻘﻧ
يﺎﻫ ﻮﻟﻮﯾﺰﯿﻓ ژ ﺨﻣ ﯽﮑﯾ ﺘ ﺖﺳا ﯽﻔﻠ . ﺴﮐاﺮﭘ ﯿ زاﺪ
رد ﻦﯿﻨﮕﯿﻟ ﻪﯾﺰﺠﺗ و ﺰﺘﻨﺳﻮﯿﺑ ﺐﺟﻮﻣ ﻮﻤﻧ و ﺪﺷر ﺪﻨﯾآﺮﻓ رد ﻪﺑ ﺦﺳﺎﭘ ،ﯽﻟﻮﻠﺳ هراﻮﯾد ﺶﻨﺗ
يﺎﻫ و ﯽﻄﯿﺤﻣ و هﺪﺷ تاﺰﻠﻓ
ﻋ ﺮﺑ ﯽﺘﻇﺎﻔﺣ ﺶﻘﻧ ياراد نژﻮﺗﺎﭘ ﻪﯿﻠ
ﺎﻫ ﺰﯿﻧ ﯽﻣ ﺪﺷﺎﺑ رد ﻦﯿﻨﭽﻤﻫ ،
يﺰﺒﺳ و هﻮﯿﻣ ﺪﺷر و ﯽﮔﺪﯿﺳر دراد ﺶﻘﻧ ﺰﯿﻧ
] 9 - 8 [ . ﻢﯾﺰﻧآ ﻦﯾا
نﮋﯿﺴﮐا دﺎﺠﯾا ﺐﺟﻮﻣ ﻪﻧﮋﯿﺴﮐا بآ ﻪﯾﺰﺠﺗ ﺎﺑ ﯽﻣ
دﻮﺷ ﻦﯾا ﻪﮐ
نﻮﯿﺳاﺪﯿﺴﮐا و زاﺪﯿﺴﮐا لﻮﻨﻓ ﯽﻠﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ياﺮﺑ نﮋﯿﺴﮐا تﺎﺒﯿﮐﺮﺗ ﻪﺑ يﺰﺒﺳ و هﻮﯿﻣ ﻞﺧاد ﯽﻟﻮﻨﻓ تﺎﺒﯿﮐﺮﺗ هﻮﻬﻗ
يا ﮓﻧر
ﺖﺳا يروﺮﺿ .
ﯽﯾاﺬﻏ تﻻﻮﺼﺤﻣ رد ﻦﯿﻨﭽﻤﻫ ﻢﯾﺰﻧآ ﻦﯾا
يروآﺮﻓ و هﺪﺷ يروآﺮﻓ ﻧ
ﯽﻤﻌﻃ ﺪﺑ دﺎﺠﯾا رد ﯽﻤﻬﻣ ﺶﻘﻧ هﺪﺸ
دراد لﻮﺼﺤﻣ ﯽﮕﻧر ﺪﺑ و ]
10 [.
ﻦﯾاﺮﺑﺎﻨﺑ
، ﺮﯿﻏ لﺎﻌﻓ يزﺎﺳ ﻦﯾا
رد ﯽﮕﻧر ﺪﺑ و ﯽﻤﻌﻃ ﺪﺑ زا لﻮﺼﺤﻣ ﻆﻔﺣ رﻮﻈﻨﻣ ﻪﺑ ﻢﯾﺰﻧآ ﺖﺳا ﺮﯾﺰﮔﺎﻧ ﺖﻌﻨﺻ ﯿﻏ ﺎﺑ و
ﺮ ياﺮﺑ مزﻻ نﮋﯿﺴﮐا نآ يزﺎﺳ لﺎﻌﻓ
هﻮﻬﻗ يا ﺪﯿﻟﻮﺗ زاﺪﯿﺴﮐا لﻮﻨﻓ ﯽﻠﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ و ﯽﻤﯾﺰﻧآ نﺪﺷ
و هﺪﺸﻧ ﺶﻨﮐاو هﻮﻬﻗ يا ﺖﻌﻧﺎﻤﻣ ﯽﻤﯾﺰﻧآ نﺪﺷ ﯽﻣ
دﻮﺷ . POD
رد ﺖﻓﺎﺑ يﺎﻫ ﻪﺑ ﯽﻫﺎﯿﮔ ترﻮﺻ
زا ﯽﺒﯿﮐﺮﺗ ﻢﯾﺰﻧآ وﺰﯾا
يﺎﻫ
ﺖﻣوﺎﻘﻣ و دراد دﻮﺟو توﺎﻔﺘﻣ ﯽﺗراﺮﺣ ﺖﻣوﺎﻘﻣ ﺎﺑ ﻒﻠﺘﺨﻣ ﯽﺗراﺮﺣ نآ ﺎﻫ ﺖﺳا توﺎﻔﺘﻣ يﺰﺒﺳ ﻊﺒﻨﻣ و عﻮﻧ ﻪﺑ ﻪﺘﺴﺑ ]
14 -
11 .[
ﯽﺗراﺮﺣ ﺖﻣوﺎﻘﻣ و نﺎﻫﺎﯿﮔ رد ﻢﯾﺰﻧآ ﻦﯾا ﯽﻧاواﺮﻓ ﺖﻠﻋ ﻪﺑ ﻻﺎﺑ ﺮﯿﻏ ﺎﺑ و ﺖﺳا حﺮﻄﻣ ﯽﻤﯾﺰﻧآ ﺺﺧﺎﺷ ناﻮﻨﻋ ﻪﺑ نآ ي
لﺎﻌﻓ يزﺎﺳ نآ ﯽﻣ ناﻮﺗ ﻪﯿﻠﮐ دﺮﮐ ضﺮﻓ ﻢﯾﺰﻧآ
يﺎﻫ ﻦﯿﺑ زا بﺮﺨﻣ
ﻪﺘﻓر ﺪﻧا ] 15 [ . ﺮﯿﻏ زا نﺎﻨﯿﻤﻃا ﺖﻬﺟ لﺎﻌﻓ
يزﺎﺳ تراﺮﺣ ،ﻢﯾﺰﻧآ
ﮓﻨﯿﭽﻧﻼﺑ ﺎﯾ ﯽﻫد
ًﻻﻮﻤﻌﻣ زﺎﯿﻧ درﻮﻣ يﺎﻣد زا ﺮﺗﻻﺎﺑ ﯽﯾﺎﻣد رد
مﺎﺠﻧا ﻢﯾﺰﻧآ ﺐﯾﺮﺨﺗ ياﺮﺑ ﯽﻣ
دﻮﺷ .
ﺗ هزوﺮﻣا ﺎﻤ ﯾ ﺎﻫاﺬﻏ فﺮﺼﻣ ﻪﺑ ﻞ ي
ﻈﻧ ﻢﻟﺎﺳ و هدﺎﻣآ ﯿ
،ﺎﻫوﺮﺴﻨﮐ ﺮ
هﺮﺘﻧﺎﺴﻨﮐ ﺰﺒﺳ ،ﺎﻫ ﯾ و فﺮﺼﻣ هدﺎﻣآ تﺎﺠ ...
اﺰﻓا ﯾ ﺶ ﯾ و ﺖﺳا ﻪﺘﻓﺎ
اﯾ ﺎﺑ تﻻﻮﺼﺤﻣ ﻦ ﯾ
ﺘﺴ ﯽ ا ﺮﺑ هوﻼﻋ ﯾ
ﻨﻤ ﯽ
، اراد ي و ﺮﻄﻋ صاﻮﺧ
ﻤﻌﻃ ﯽ شزرا و ﮓﻧر ،ﺖﻓﺎﺑ ، ﻪﯾﺬﻐﺗ
يا ﺒﻃ ﯿﻌ ﯽ هدﻮﺑ رﺎﻋ و ي زا
ﺮﺨﺗ و دﺎﺴﻓ ﻞﻣاﻮﻋ ﯾ
ﺐ ﺰﯿﻧ ﺪﻨﺷﺎﺑ ] 16 [ . شور يﺎﻫ ﻠﺘﺨﻣ ياﺮﺑ ﯽﻔ
ﯽﺗراﺮﺣ ﺮﯿﻏ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ﻢﯾﺰﻧآ
يﺎﻫ هﺪﺷ هدﺎﻔﺘﺳا ﯽﻫﺎﯿﮔ
ﻪﮐ ﺖﺳا ﺖﯾدوﺪﺤﻣ ﺎﻫ
ﺪﻧراد ار دﻮﺧ صﺎﺧ يﺎﯾاﺰﻣ و .
ﻪﺑ ناﻮﻨﻋ
ﻻﺎﺑ ﮏﯿﺗﺎﺘﺳاورﺪﯿﻫ رﺎﺸﻓ ﺪﻨﯾآﺮﻓ ﯽﯾارﺎﮐ ﯽﻘﯿﻘﺤﺗ رد لﺎﺜﻣ ) (HHP رد ﺐﯿﮐﺮﺗ ﺎﺑ ﺮﺑ ﻢﯾﻼﻣ ﯽﺗراﺮﺣ ﺪﻨﯾاﺮﻓ لﺎﻌﻓ ﺮﯿﻏ
يزﺎﺳ
ﻢﯾﺰﻧآ يﺎﻫ زاﺪﯿﺴﮐاﺮﭘ )
(POD
،ﺞﯾﻮﻫ رد دﻮﺨﻧ و ﺰﺒﺳ يﺎﯿﺑﻮﻟ
ﺪﺷ ﯽﺳرﺮﺑ ﺰﺒﺳ .
،لوا ﻪﻠﺣﺮﻣ رد ﻪﻧﻮﻤﻧ
ﺎﻫ رﺎﺸﻓ ﺖﺤﺗ 450
- 250
ﮕﻣ ﺎ يﺎﻣد و لﺎﮑﺳﺎﭘ 50
- 50 تﺪﻣ ﻪﺑ 15
- 60 راﺮﻗ ﻪﻘﯿﻗد
ﺪﻨﺘﻓﺮﮔ . ود رﺎﻤﯿﺗ ،ﻖﯿﻘﺤﺗ مود ﻪﻠﺣﺮﻣ رد ﻪﻠﺣﺮﻣ
يا ترﻮﺻ ﻪﺑ
يﺎﻣد ﺎﺑ بآ رد ﭻﻧﻼﺑ 40
- 70 ﻪﺟرد ﯽﺘﻧﺎﺳ داﺮﮔ تﺪﻣ ﻪﺑ 15 و
30 رﺎﺸﻓ ﺪﻨﯾآﺮﻓ ﺲﭙﺳ و ﻪﻘﯿﻗد 250
ﮕﻣ ﺎ و لﺎﮑﺳﺎﭘ 20
ﻪﺟرد
ﯽﺘﻧﺎﺳ داﺮﮔ تﺪﻣ ﻪﺑ 15
- 60 ﺪﺷ مﺎﺠﻧا ﻪﻘﯿﻗد .
راﺪﻘﻣ ﺞﯾﻮﻫ رد
لﺎﻤﻋا زا ﺲﭘ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ 30
رﺎﺸﻓ رﺎﻤﯿﺗ ﻪﻘﯿﻗد
350 ﮕﻣ ﺎ يﺎﻣد رد لﺎﮑﺳﺎﭘ 20
ﻪﺟرد ﯽﺘﻧﺎﺳ داﺮﮔ ﻪﺑ 16 ﺖﯿﻟﺎﻌﻓ ٪
ﺎﺑ و ﺖﻓﺎﯾ ﺶﻫﺎﮐ ﻪﯿﻟوا ﯽﻧﻻﻮﻃ
ﺮﺗ ﻪﺑ ﻻﺎﺑ رﺎﺸﻓ لﺎﻤﻋا نﺎﻣز ندﺮﮐ
60 ﯿﻗد ﻪﺑ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ راﺪﻘﻣ ﻪﻘ 9
ﺖﻓﺎﯾ ﺶﻫﺎﮐ ﻪﯿﻟوا راﺪﻘﻣ ٪ .
،ﺰﺒﺳ يﺎﯿﺑﻮﻟ ياﺮﺑ ﻦﯾﺮﺗﺮﺛﺆﻣ
ود رﺎﻤﯿﺗ لﺎﻤﻋا ﺎﺑ ﺞﯾﺎﺘﻧ ﻪﻠﺣﺮﻣ
يا ﻪﺑ
ﺰﺒﺳ دﻮﺨﻧ رد ﺎﻣا ﺪﻣآ ﺖﺳد لﺎﻌﻓ ﺮﯿﻏ
ﻪﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ يزﺎﺳ
ﺪﻣﺎﯿﻧ ﺖﺳد ﻪﺑ يﺮﺛﻮﻣ ترﻮﺻ ]
17 [ . ﻦﯿﻨﭽﻤﻫ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﺎﯿﺣا و نﻮﯿﺳاﺪﯿﺴﮐا ﺖﯿﻫﺎﻣ ﯾ
ﯽ ﺖﯿﻟﺎﻌﻓ ندﻮﺑ ﻢﯾﺰﻧآ
يﺎﻫ زاﺪﯿﺴﮐاﺮﭘ
،زاﺪﯿﺴﮐا لﻮﻨﻓ ﯽﻠﭘ و ﯽﻫﺎﯿﮔ يﺎﻫ ناﺪﯿﺴﮐا ﯽﺘﻧآ زا هدﺎﻔﺘﺳا
ﯽﻣ ﺪﻧاﻮﺗ ﺐﺳﺎﻨﻣ هﺪﯾا ﮏﯾ ناﻮﻨﻋ ﻪﺑ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ رد
رد زاﺪﯿﺴﮐاﺮﭘ هﻮﯿﻣ
ﺎﻫ و تﺎﺠﯾﺰﺒﺳ ﺪﺷﺎﺑ حﺮﻄﻣ
. ﻪﻨﯿﻣز ﻦﯾا رد
ﺮﯿﻈﻧ ﯽﻔﻠﺘﺨﻣ تﺎﻘﯿﻘﺤﺗ ﯽﺳرﺮﺑ
ﺮﯿﺛﺄﺗ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﻦﺸﯾوآ ﺲﻧﺎﺳا
ﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺰﺒﺳ ﺎﯿﺑﻮﻟ و هﺎﯿﺳ بﺮ
] 18 [ ، ﺮﯿﺛﺄﺗ ﺲﻧﺎﺳا
ﻪﭽﺑﺮﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ﺮﺑ ﮏﺨﯿﻣ ]
19 [ ، ﺮﯿﻏ
يزﺎﺳ لﺎﻌﻓ ﻢﯾﺰﻧآ
زاﺪﯿﺴﮐاﺮﭘ ﺖﺤﺗ ﺰﻣﺮﻗ ﻢﻠﮐ ﺮﯿﺛﺄﺗ
ﺲﻧﺎﺳا يﺎﻫ
ﺰﺒﺳ هﺮﯾز و ﻪﻧﺎﯾزار ،ﮏﺨﯿﻣ ]
20 [ ، ﺮﯿﺛﺄﺗ ﺲﻧﺎﺳا يﺎﻫ ،ﺮﭙﻠﮔ
ﻢﯾﺰﻧآ ﺮﺑ ﮏﺨﯿﻣ و ﻦﺸﯾوآ ﺲﻓﺮﮐ زاﺪﯿﺴﮐاﺮﭘ
] 21 [ ﺳﻮﺗ ﻂ
ﺨﻣ ﻦﯿﻘﻘﺤﻣ ﻒﻠﺘ
ﻪﺘﻓﺮﮔ ترﻮﺻ ﺖﺳا
. نﺎﺸﻧ تﺎﻘﯿﻘﺤﺗ ﻦﯾا ﺞﯾﺎﺘﻧ
ﻪﮐ داد ﺶﻨﮐاو رﺎﺘﻓر و ﻪﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺲﻧﺎﺳا
يﺎﻫ ﻒﻠﺘﺨﻣ
دﻮﺑ توﺎﻔﺘﻣ ﯽﻤﯾﺰﻧآ ﻊﺒﻨﻣ عﻮﻧ سﺎﺳا ﺮﺑ .
ﺲﻧﺎﺳا ﻪﮐ يرﻮﻃ ﻪﺑ
ﻦﺸﯾوآ ﺐﯿﺗﺮﺗ ﻪﺑ ﺰﺒﺳ ﺎﯿﺑﻮﻟ و هﺎﯿﺳ بﺮﺗ رد ﺶﻫﺎﮐ ﺐﺟﻮﻣ
و
ﺖﯿﻟﺎﻌﻓ ﺪﯾﺪﺸﺗ دﻮﺑ هﺪﺷ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ
. ﻦﯿﻨﭽﻤﻫ ﻘﻘﺤﻣ ﻦﯾا ﻦﯿ
ار زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺖﻠﻋ ﻪﺑ
ﯽﺘﻧآ ﺖﯿﺻﺎﺧ
ﺲﻧﺎﺳا ﯽﻧاﺪﯿﺴﮐا درﻮﻣ يﺎﻫ
ﻂﺒﺗﺮﻣ هدﺎﻔﺘﺳا ﺪﻨﺘﺴﻧاد
. ﺖﻘﯿﻘﺣ رد
ﺲﻧﺎﺳا نﻮﯿﺳاﺪﯿﺴﮐا ياﺮﺑ زﺎﯿﻧ درﻮﻣ نﮋﯿﺴﮐا ﺎﺑ نﺪﺷ ﺐﯿﮐﺮﺗ ﺎﺑ ﺎﻫ
ﯽﻟﻮﻨﻓ تﺎﺒﯿﮐﺮﺗ )
لﻮﮐﺎﯾﺎﮔ ( ﮓﻧر دﺎﺠﯾا ﻊﻧﺎﻣ و هدﺮﮐ فﺬﺣ ار
هﻮﻬﻗ ﯽﻣ يا ﺪﻧﻮﺷ . ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ ﺖﯿﺻﺎﺧ ﯽﺑوﺮﮑﯿﻣ ﯽﺘﻧآ و
ﺲﻧﺎﺳا يﺎﻫ داﻮﻣ ﻪﺑ ﻒﻠﺘﺨﻣ هﺮﺛﺆﻣ
رد دﻮﺟﻮﻣ نآ
ﺎﻫ دراد ﯽﮕﺘﺴﺑ
رد داﻮﻣ ﻦﯾا راﺪﻘﻣ و عﻮﻧ زا ﯽﻫﺎﮔآ ﻦﯾاﺮﺑﺎﻨﺑ ﺲﻧﺎﺳا
ﺎﻫ ﯽﻣ ﺪﻧاﻮﺗ
ﺪﻨﮐ ﮏﻤﮐ ﺲﻧﺎﺳا ﯽﮔﺪﻧرادزﺎﺑ ﺮﺛا ﻢﺴﯿﻧﺎﮑﻣ ﺮﺘﻬﺑ كرد ﻪﺑ .
ﺲﻧﺎﺳا
،لﻮﻧژوا ﺮﯿﻈﻧ يراﺮﻓ تﺎﺒﯿﮐﺮﺗ ياراد ﮏﺨﯿﻣ ﻞﮑﻟا ،ﻦﯿﻠﯿﻓﻮﺑرﺎﮐ
وﺰﻨﺑ ،ﮏﯿﻠﯾﺰﻨﺑ ﯽﻣ ﻦﻠﯿﺗا و لوﻮﻓرﻮﻓ ،ﻞﯿﺘﻣ ود تا
لﻮﻧژوا ﻪﮐ ﺪﺷﺎﺑ
ﯽﻣ ﮏﺨﯿﻣ ﯽﻠﺻا هدﺎﻣ ﺪﺷﺎﺑ
. لﻮﻨﯿﻣﻮﮐ ﺰﺒﺳ هﺮﯾز هﺮﺛﺆﻣ هدﺎﻣ
ﯽﻣ ﺪﺷﺎﺑ و ﺖﺳا يﺪﯿﺋﺪﻟآ ﯽﺒﯿﮐﺮﺗ ﻪﮐ دوﺪﺣ
30 - 50 ﺪﺻرد
راﺮﻓ تﺎﺒﯿﮐﺮﺗ ﺰﺒﺳ هﺮﯾز
ﯽﻣ ﻞﯿﮑﺸﺗ ار ﺪﻫد
. ﻪﻧاد ﺲﻧﺎﺳا رد
دوﺪﺣ ﻪﻧﺎﯾزار 40
ﻪﮐ ﺖﺳا هﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺐﯿﮐﺮﺗ ﺎﺘﺑ
- ،ﻦﺳﺮﯿﻣ
ﺎﻔﻟآ ﺎﻔﻟآ ،ﻦﻧﻮﻤﯿﻟ ،نروﺪﻧﻼﻓ ، -
ﻦﻨﯿﭘ هﺪﺷ ﯽﺳﺎﻨﺷ ﺐﻟﺎﻏ تﺎﺒﯿﮐﺮﺗ
ﯽﻣ ﺪﻨﺷﺎﺑ . هﺪﺷ ﺪﯿﻗ ﺐﻟﺎﻄﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ و
فﺮﺼﻣ ﻞﯾﺎﻤﺗ هﺪﻨﻨﮐ
ﺎﻫ
زا يرﺎﻋ و يروآﺮﻓ ﻞﻗاﺪﺣ ﺎﺑ يﺎﻫاﺬﻏ فﺮﺼﻣ ﻪﺑ ﻪﮕﻧ
هﺪﻧراد يﺎﻫ
ﯽﺳرﺮﺑ فﺪﻫ ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦﯾا ﯽﯾﺎﯿﻤﯿﺷ ﺮﯿﺛﺄﺗ
زا هدﺎﻔﺘﺳا ﺲﻧﺎﺳا
يﺎﻫ
ﻪﻧﺎﯾزار و هﺮﯾز ،ﮏﺨﯿﻣ ﺮﯿﻏ رد
ﻌﻓ لﺎ يزﺎﺳ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ وﺪﮐ
ﺰﺒﺳ مﺎﺠﻧا .ﺪﺷ
2 - شور و داﻮﻣ ﺎﻫ
2 - 1 - هدﺎﻔﺘﺳا درﻮﻣ داﻮﻣ
ﻦﯾا رد ﻪﻌﻟﺎﻄﻣ وﺪﮐ ﺰﺒﺳ ﻊﺒﻨﻣ ناﻮﻨﻋ ﻪﺑ ﻢﯾﺰﻧآ
ﺴﮐاﺮﭘ ﯿ زاﺪ بﺎﺨﺘﻧا
يﺰﺒﺳ و هﻮﯿﻣ رازﺎﺑ زا و ناﺪﻤﻫ
) ناﺮﯾا ( ﺪـﺷ ﻪﯿﻬﺗ . ﺲﻧﺎـﺳا يﺎـﻫ
ﺖﻈﻠﻏ و ﻪﯿﻬﺗ ﺲﻧﺎﺳا ﺞﯾرﺎﺑ ﺖﮐﺮﺷ زا ﻪﻧﺎﯾزار و هﺮﯾز ،ﮏﺨﯿﻣ -
يﺎﻫ 50 ، 75 ، 100 و 200ppm نآ ﻦﯾﻮـﺗ زا هدﺎﻔﺘـﺳا ﺎﺑ ﺎﻫ 80
) نﺎﻤﻟآ ،كﺮﻣ (
ﺮﯾﺎﻔﯿﺴﻟﻮﻣا ناﻮﻨﻋ ﻪﺑ نآ ﺮﯿﺛﺄﺗ و ﻪﯿﻬﺗ
ﺮﺑ ﺎﻫ ﻟﺎﻌﻓ ﯿ ﺖ
زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ وﺪﮐ
ﺰﺒﺳ ﺳرﺮﺑ ﯽ ﺪﺷ .
2 - 2 - شور ﺎﻫ
2 - 2 - 1 - ﯽﻤﯾﺰﻧآ هرﺎﺼﻋ ﻪﯿﻬﺗ
ياﺮﺑ ﻬﺗ ﯿ ﺰﻧآ هرﺎﺼﻋ ﻪ ﯾﻤ
ﯽ 5 مﺮﮔ وﺪﮐ ﺰﺒﺳ ندوﺰـﻓا ﺎﺑ 15
ﯽـﻠﯿﻣ
ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟ )
4 رد ﯽﺘﻧﺎﺳ ﻪﺟ داﺮﮔ ( دﺮﺧ و ﻞﺻﺎﺣ طﻮﻠﺨﻣ ﻪـﺑ
تﺪﻣ 15 يﺎﻣد رد ﻪﻘﯿﻗد 4
ﯽﺘﻧﺎﺳ ﻪﺟرد ژﻮﻔﯾﺮﺘﻧﺎـﺳ داﺮﮔ
) rpm
10000 ( و ﺪﺷ زﺎﻓ ﯽﯾور هدﺎﻔﺘﺳا درﻮﻣ ﯽﻤﯾﺰﻧآ هرﺎﺼﻋ ناﻮﻨﻋ ﻪﺑ
ﺖﻓﺮﮔ راﺮﻗ ]
18 .[
2 - 2 - 2 - طﻮﻠﺨﻣ ياﺮﺘﺴﺑﻮﺳ ﺶﻨﮐاو
اﺮﺘﺴﺑﻮﺳ طﻮﻠﺨﻣ ي
ﺎﻣزآ ﯾ ﺶ ﻞﻣﺎﺷ 10 لﻮﮐﺎـﯾﺎﮔ ﺮـﺘﯿﻟ ﯽﻠﯿﻣ 1
% ،
10 ﺮﺘﯿﻟ ﯽﻠﯿﻣ بآ
ﻪﻧﮋﯿﺴﮐا 3 % و 100 تﺎﻔﺴـﻓ ﺮﻓﺎـﺑ ﺮـﺘﯿﻟ ﯽـﻠﯿﻣ
ﻪﮐ دﻮﺑ ﻢﯾﺪﺳ ﻬﺗ زا ﺲﭘ
ﯿﻪ نآpH يور 5/
6 ﺪﺷ ﻢﯿﻈﻨﺗ ]
22 .[
2 - 2 - 3 - هزاﺪﻧا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺮﯿﮔ
هزاﺪﻧا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺮﯿﮔ وﺪﮐ
ﺰﺒﺳ شور سﺎـﺳا ﺮﺑ
نارﺎﮑﻤﻫ و ﺲﻧﻮﭘ )
2004 ( رﻮـﻈﻨﻣ ﻦـﯾا ياﺮﺑ ﺪﺷ مﺎﺠﻧا
، راﺪـﻘﻣ
87 /2 ﯿﻟ ﯽﻠﯿﻣ ،اﺮﺘﺴﺑﻮﺳ طﻮﻠﺨﻣ ﺮﺘ 1/
0 ﯽﻠﯿﻣ مﺎﺧ هرﺎﺼﻋ ﺮﺘﯿﻟ وﺪﮐ
ﺰﺒﺳ و 03 /0 ًﺎﻌﻤﺟ ﻪﮐ ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟ ﯽﻠﯿﻣ 3
ﯽﻠﯿﻣ ﻪـﺑ دﻮـﺑ ﺮـﺘﯿﻟ
و ﻞﻘﺘﻨﻣ توﺎﮐ ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا زا هدﺎﻔﺘﺳا ﺎﺑ
ءاروﺎـﻣ ﯽﯾﻮـﺗﺮﭘ ود
ﺶﻔﻨﺑ - ﯽﺋﺮﻣ ) نﺎﺘﺴﻠﮕﻧا ،ﺖﻨﻣوﺮﺘﺴﻨﯾا ﯽﺟ ﯽﭘ (
جﻮـﻣ لﻮـﻃ رد ،
470 ﻂﯿﺤﻣ يﺎﻣد رد و ﺮﺘﻣﻮﻧﺎﻧ )
30 ﻪﺟرد ﯽﺘﻧﺎﺳ ـﮔ داﺮ ( بﺬـﺟ
ﻪﻧﻮﻤﻧ ﺪﺷ ﺖﺋاﺮﻗ ﯽﮑﯿﺘﻨﯿﺳ ترﻮﺻ ﻪﺑ ﺎﻫ ]
22 .[
ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ
يﺎﻫ
،زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﯽﻫﺎﯿﮔ ندوﺰﻓا ﺎﺑ
03 /0 زا ﺮﺘﯿﻟ ﯽﻠﯿﻣ
ﺖﻈﻠﻏ ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ يﺎﻫ
ﺎﻫ ﯽﻫﺎﯿﮔ ي يﺎـﺟ ﻪـﺑ ، 03
/0 ﯽـﻠﯿﻣ
ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟ )
قﻮـﻓ طﻮﻠﺨﻣ رد (
و توﺎـﮐ ﻪـﺑ ﺖـﺋاﺮﻗ ﺲﭙـﺳ
بﺬﺟ ﻪﻧﻮﻤﻧ رد ﺎﻫ جﻮﻣ لﻮﻃ 470
ﻣﻮﻧﺎﻧ ﺮﺘ ﺪﺷ ﯽﺳرﺮﺑ .
2 - 3 - ﻪﻨﯿﻬﺑ تﺎﺸﯾﺎﻣزآ يزﺎﺳ
ﻪﻨﯿﻬﺑ ﺲﻧﺎـﺳا ﺮﯿﺛﺄـﺗ ﺖـﺤﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يزﺎﺳ
يﺎـﻫ
ﺦـﺳﺎﭘ ﺢﻄﺳ شور زا هدﺎﻔﺘﺳا ﺎﺑ ﯽﻫﺎﯿﮔ )
راﺰـﻓا مﺮـﻧ Design
Expert 6.0.2 (
و يﺰﮐﺮﻣ ﺐﮐﺮﻣ حﺮﻃ )
1CCD
( ﺎﺑ 3 ﺢﻄـﺳ
و 5 يﺰﮐﺮﻣ ﻪﻄﻘﻧ رد راﺮﮑﺗ ﺪﺷ مﺎﺠﻧا
) 1 + ، 0 ، 1 - ) ( لوﺪﺟ 1 .(
هدوﺪﺤﻣ ي ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﻞﻘﺘﺴﻣ يﺎﻫﺮﯿﻐﺘﻣ
1) (X ﺖﻈﻠﻏ ،
ﺲﻧﺎﺳا هدﺎﻔﺘﺳا درﻮﻣ يﺎﻫ )
X2
( نﻮـﻣزآ زا جﺎﺘﻨﺘـﺳا ﻪـﯿﻟوا يﺎـﻫ
ﺪﯾدﺮﮔ . لﺪﻣ ﻪﻠﻤﺟ ﺪﻨﭼ ﯽﻧﻮﯿﺳﺮﮔر يﺎﻫ
رﻮﻈﻨﻣ ﻪﺑ مود ﻪﺟرد يا
ﺶﯿﭘ ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ،ﺦﺳﺎﭘ ﯽﻨﯿﺑ ﻪﮐ
ﻪـﻟدﺎﻌﻣ ترﻮـﺻ ﻪـﺑ )
1 (
ﯽﻣ ﺪﺷﺎﺑ : ﺴـﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ) ﻪﺘﺴﺑاو ﺮﯿﻐﺘﻣ Y ﻪﮐ ترﻮـﺻ ﻪـﺑ ﻪـﮐ زاﺪﯿ
ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا بﺬﺟ (
ﯽﻣ ،ﺪﺷﺎﺑ β0
ﺐﯾﺮﺿ ﺖﺑﺎﺛ لﺪـﻣ و βi
و
βii
و βij
ﺖﺑﺎﺛ ﺐﯾاﺮﺿ لﺪﻣ ﻂﺳﻮﺗ هﺪﺷ دروآﺮﺑ
يﺎﻫﺮﯿﻐﺘﻣ ياﺮﺑ
ﻞﻘﺘﺴﻣ xi
و xj
ﻞﺑﺎﻘﺘﻣ تاﺮﺛا و نآ
ﺎﻫ ﻫ ﺪﻨﺘﺴ .
Table 1 Independent variables and their applied levels for optimizing peroxidase enzyme activity in courgette under effect of
different essential oils
Variables level +1 0
Independent variables -1
400 200
0 The time of enzyme activity (s)
200 100
0 Essential oil concentration (ppm)
2 - 4 - يرﺎﻣآ ﻞﯿﻠﺤﺗ و ﻪﯾﺰﺠﺗ
ﺎﭘ ﺮﺑ ﻞﯾرﻮﺘﮐﺎﻓ ترﻮﺻ ﻪﺑ ﻖﯿﻘﺤﺗ ﻦﯾا و ﯽﻓدﺎﺼـﺗ ًﻼﻣﺎﮐ حﺮﻃ ﻪﯾ
ﺲﻧﺎﺳا ﺖﻈﻠﻏ و عﻮﻧ ﺮﯿﺛﺄﺗ ﯽﺳرﺮﺑ رﻮﻈﻨﻣ ﻪﺑ راﺮﮑﺗ ﻪﺳ رد يﺎـﻫ
ﺪﺷ مﺎﺠﻧا ﺰﺒﺳ وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﯽﻌﯿﺒﻃ .
ﻪﺟﻮﺗ ﺎﺑ
ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﮑﻨﯾا ﻪﺑ
ﯽـﻣ ﯽﮑﯿﺘﻨﯿـﺳ ترﻮﺻ ﻪﺑ ﺎﻫ ياﺮـﺑ ،ﺪـﺷﺎﺑ
1. Central Composite Design
ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﯽﺳرﺮﺑ
ﻢﯾﺰـﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
ﮐ زاﺪﯿﺴﮐاﺮﭘ ﯽﻨﺤﻨﻣ ،ﺰﺒﺳ وﺪ
ﻪـﺑ زاﺪﯿﺴـﮐاﺮﭘ ﻢﯾﺰـﻧآ ﺖـﯿﻟﺎﻌﻓ يﺎﻫ
ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ﺎﺑ و ﻢﺳر ﯽﮑﯿﺘﻨﯿﺳ ترﻮﺻ )
ﺲﻧﺎﺳا نوﺪﺑ (
ﻪﺴﯾﺎﻘﻣ
ﺪﻧﺪﺷ ) 05 /0
<
.(p راﺰـﻓا مﺮـﻧ زا ﺎـﻫرادﻮﻤﻧ ﻢﺳر ياﺮﺑ )
2007 (
Excel ﺪﺷ هدﺎﻔﺘﺳا .
3 - ﺚﺤﺑ و ﺞﯾﺎﺘﻧ
3 - 1 - وﺪـﮐ زاﺪﯿﺴـﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ تاﺮﯿﯿﻐﺗ
ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﺖﺤﺗ ﺰﺒﺳ
ﺒﺳ هﺮﯾز يﺎﻫ و ﻪﻧﺎﯾزار ،ﺰ
ﮏﺨﯿﻣ
نﺎﻤﻫ ﻞﮑﺷ رد ﻪﮐ رﻮﻃ )
1 ( ﯽﻣ ﻪﻈﺣﻼﻣ و ﺺﻟﺎﺧ مﺮﻓ ﺎﻬﻨﺗ دﻮﺷ
ﺖﻈﻠﻏ 200ppm
ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺚﻋﺎﺑ ﺰﺒﺳ هﺮﯾز ﺲﻧﺎﺳا
ﺖﺳا هﺪﺷ ﺰﺒﺳ وﺪﮐ رد زاﺪﯿﺴﮐاﺮﭘ .
و ﺺﻟﺎﺧ مﺮﻓ ﻦﯿﻨﭽﻤﻫ
ﺖﻈﻠﻏ يﻻﺎﺑ يﺎﻫ )
100 و 200 ppm ( ﯽﯾﺎﻧاﻮﺗ ،ﻪﻧﺎﯾزار ﺲﻧﺎﺳا
وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺎﻣا ﺪﻧدﻮﺑ اراد ار ﺰﺒﺳ
ﺖﻈﻠﻏ زا هدﺎﻔﺘﺳا يﺎﻫ
50 و 75 ppm ﺚﻋﺎﺑ ﻪﻧﺎﯾزار ﺲﻧﺎﺳا
ﺖﺳا هﺪﺷ ﺰﯿﻧ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا .
ﮏﺨﯿﻣ ﺲﻧﺎﺳا
ﺖﻈﻠﻏ ﻪﯿﻠﮐ رد ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
ﺪﻧﺪﺷ زاﺪﯿﺴﮐاﺮﭘ ﻪﮐ
ﺮﮕﻧﺎﯿﺑ ﺶﻫﺎﮐ ﻦﯾا ياﺮﺘﺴﺑﻮﺳ ﺶﻫﺎﮐ
ﯽﻓﺮﺼﻣ و ﺎﯾ ﺶﻫﺎﮐ نﮋﯿﺴﮐا درﻮﻣ زﺎﯿﻧ ﻢﯾﺰﻧآ دﺎﺠﯾا ياﺮﺑ ﮓﻧر
هﻮﻬﻗ يا ﯽﻣ ﺪﺷﺎﺑ ﻪﮐ نﺎﺸﻧ هﺪﻨﻫد ﯽﯾﺎﻧاﻮﺗ رد ﺲﻧﺎﺳا ﻦﯾا ﺶﻨﮐاو
ﺎﺑ نﮋﯿﺴﮐا ﻂﯿﺤﻣ و ﺖﻌﻧﺎﻤﻣ زا ﺶﻨﮐاو زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ و
ﯽﻠﭘ
لﻮﻨﻓ زاﺪﯿﺴﮐا ) هﻮﻬﻗ يا نﺪﺷ ﯽﻤﯾﺰﻧآ ( ﯽﻣ ﺪﺷﺎﺑ . ﺞﯾﺎﺘﻧ ﺮﯾﺎﺳ
ﺮﮕﻧﺎﯿﺑ ﺰﯿﻧ ﻦﯿﻘﻘﺤﻣ ﺮﯿﺛﺄﺗ
ﺖﺒﺜﻣ ﺲﻧﺎﺳا ﮏﺨﯿﻣ رد ﺖﻌﻧﺎﻤﻣ زا
ﺖﯿﻟﺎﻌﻓ ﯾﺰﻧآ زاﺪﯿﺴﮐاﺮﭘ ﻢ رد
ﻢﻠﮐ ﺪﯿﻔﺳ و ﻢﻠﮐ دﻮﺑ ﺰﻣﺮﻗ و ﺮﺛا
ﺖﻌﻧﺎﻤﻣ ﯽﮔﺪﻨﻨﮐ ﺲﻧﺎﺳا ﺎﺑ ﺶﯾاﺰﻓا ﺖﻈﻠﻏ ﺪﯾﺪﺸﺗ ﺲﻧﺎﺳا ﯽﻣ
-
ﺪﺷ ] 23 .[
ﻞﮑﺷ 2 ﯽﻨﺤﻨﻣ يﺪﻌﺑ ود و يﺪﻌﺑ ﻪﺳ يﺎﻫ )
رﻮﺘﻨﮐ ( ﻢﻫ ﺮﯿﺛﺄﺗ نﺎﻣز
ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،هﺮﯾز يﺎﻫ
ﺒﺳ وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ناﺰﯿﻣ ﺮﺑ ﯽﻤﯾﺰﻧآ نﺎﺸﻧ ار ﺰ
ﯽﻣ ﺪﻫد . نﺎﻤﻫ ﺶﺨﺑ رد ﻪﮐ رﻮﻃ يﺎﻫ
) (A و ) (B ﻞﮑﺷ 2
ﯽﻣ ﻪﻈﺣﻼﻣ ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ﺎﺑ دﻮﺷ
ﻪﻧﺎﯾزار و هﺮﯾز يﺎﻫ
ﯽﻣ نﺎﺸﻧ ار ﯽﺸﯾاﺰﻓا ﺪﻧور زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ناﺰﯿﻣ ﺪﻫد
ﺶﻨﮐاو نﺎﻣز نﺪﺷ ﯽﻧﻻﻮﻃ ﺎﺑ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﻦﯾا ﻪﮐ ﯽﻣ دﻮﻬﺸﻣ ﺮﺘﺸﯿﺑ ﺪﺷﺎﺑ
. اﺰﻓا ﺎﺑ ﻦﯿﻨﭽﻤﻫ ،ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﺶﯾ
ﯽﻣ ﺶﯾاﺰﻓا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺎﻬﺘﻧا رد ﻪﮐ ﺪﻨﭼﺮﻫ ﺪﺑﺎﯾ
سﺮﺘﺳد رد ياﺮﺘﺴﺑﻮﺳ نﺪﺷ ﻢﮐ ﺖﻠﻋ ﻪﺑ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ناﺰﯿﻣ ،ﻢﯾﺰﻧآ ﻮﯿﺗاﺪﯿﺴﮐا ﺖﯿﻟﺎﻌﻓ نﺪﺷ ﻢﮐ و ﻢﯾﺰﻧآ ﻪﺘﺴﻫآ ﯽﻟوﺰﻧ و ﺖﺑﺎﺛ ًﺎﺒﯾﺮﻘﺗ ﺪﻧور ﯽﻣ نﺎﺸﻧ ار يا
ﺪﻫد ] 25 - 24 .[
نﺎﻤﻫ ﻃ ﺶﺨﺑ رد ﻪﮐ رﻮ )
و C1 (C2 ﻞﮑﺷ 2 رد ﺖﺳا ﺺﺨﺸﻣ
،ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﺶﯾاﺰﻓا ﺎﺑ ،ﮏﺨﯿﻣ ﺲﻧﺎﺳا ﺖﺑﺎﺛ ﺖﻈﻠﻏ ﯽﻣ ﺶﯾاﺰﻓا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﯽﻣ ﻪﮐ ﺪﺑﺎﯾ
ﺖﻠﻋ ﻪﺑ ﺪﻧاﻮﺗ
ﺮﯾﺎﺳ ﺞﯾﺎﺘﻧ ﺎﺑ ﻪﮐ ﺪﺷﺎﺑ اﺮﺘﺴﺑﻮﺳ ﻪﺑ ﻢﯾﺰﻧآ ﯽﺳﺮﺘﺳد ﺶﯾاﺰﻓا دراد ﺖﻘﺑﺎﻄﻣ ﺰﯿﻧ ﻦﯿﻘﻘﺤﻣ .
ﻪﻧﻮﮔ ﻪﺑ ﺎﻣز ﻦﯾﺮﺗﻻﺎﺑ رد ﻪﮐ يا ن
ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻦﯾﺮﺘﺸﯿﺑ هﺪﺷ بﺎﺨﺘﻧا ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ )
بﺬﺟ (
ﯽﻣ هﺪﻫﺎﺸﻣ دﻮﺷ
. نﺎﻤﻫ ﯽﻣ هﺪﻫﺎﺸﻣ ﻪﮐ رﻮﻃ نﺎﻣز رد دﻮﺷ
يﺎﻫ
ﻪﺑ ﺮﺠﻨﻣ ﮏﺨﯿﻣ ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ،ﯽﻤﯾﺰﻧآ ﺶﻨﮐاو ﻪﯿﻟوا ﯽﻣ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﺎﺑ ماﻮﺗ ﺖﺑﺎﺛ ﺮﯿﯿﻐﺗ دﻮﺷ
. رد
نﺎﻣز ﯽﻧﻻﻮﻃ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ يﺎﻫ ﻠﻏ و ﺮﺗ
ﺖﻈ ،ﺲﻧﺎﺳا ﺮﺗﻻﺎﺑ يﺎﻫ
هﻮﻬﻗ ﺶﻨﮐاو زا ﻂﯿﺤﻣ نﮋﯿﺴﮐا ﺎﺑ ﺲﻧﺎﺳا نﺪﺷ ﺐﯿﮐﺮﺗ ﻞﯿﻟد ﻪﺑ يا
زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﺠﯿﺘﻧ رد و هﺪﺷ ﺖﻌﻧﺎﻤﻣ ﯽﻤﯾﺰﻧآ نﺪﺷ ﯽﻣ ﺶﻫﺎﮐ ﺪﺑﺎﯾ
] 20 .[
و تﺪﺷ زا ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﺶﯾاﺰﻓا ﺎﺑ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ خﺮﻧ
) بﺬﺟ ( ﯽﻣ ﻪﺘﺳﺎﮐ ﺶﻫﺎﮐ ﻦﯾا ﻪﮐ دﻮﺷ
ﯽﻣ ﺖﻠﻋ ﻪﺑ ﺪﻧاﻮﺗ ﻢﮐ و ﻢﯾﺰﻧآ سﺮﺘﺳد رد ياﺮﺘﺴﺑﻮﺳ نﺪﺷ ﻢﮐ
ﻞﯿﮑﺸﺗ ﺮﮕﯾد يﻮﺳ زا ؛ﺪﺷﺎﺑ ﻢﯾﺰﻧآ ﻮﯿﺗاﺪﯿﺴﮐا ﺖﯿﻟﺎﻌﻓ نﺪﺷ ﯽﻣ ﺰﯿﻧ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ زا ،هﺪﻨﻨﮐ ﺖﻌﻧﺎﻤﻣ تﺎﺒﯿﮐﺮﺗ ﻦﯾا رد ﺪﻧاﻮﺗ
ﺪﻨﺷﺎﺑ راﺬﮔﺮﺛا درﻮﻣ ]
25 - 24 .[
،ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﺶﯾاﺰﻓا ﺎﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا ﻦﯿﻘﻘﺤﻣ ﺮﯾﺎﺳ تﺎﻌﻟﺎﻄﻣ رد ﺖﺳا هﺪﺷ ﺖﺑﺎﺛ ﺰﯿﻧ
] 20 - 18 و 26 .[
ﯽﻧﺎﺷﺎﮐ داﮋﻧ و ﯽﺋاراد ) 2013 ( ﺰﯿﻧ رد ﯽﻘﯿﻘﺤﺗ نﺎﯿﺑ ﻪﮐ ﺪﻨﺘﺷاد
ﺲﻧﺎﺳا ﺎﻫ ﺎﺑ ﺐﯿﮐﺮﺗ نﺪﺷ ﺎﺑ نﮋﯿﺴﮐا ﻂﯿﺤﻣ ﻊﻧﺎﻣ هﻮﻬﻗ زا يا نﺪﺷ
ﯽﻤﯾﺰﻧآ هﺪﺷ و رد ﻪﺠﯿﺘﻧ ﺖﯿﻟﺎﻌﻓ ﻢﯾﺰﻧآ لﺮﺘﻨﮐ ﺖﺤﺗ ار زاﺪﯿﺴﮐاﺮﭘ
ﯽﻣ راﺮﻗ ﺪﻨﻫد ﻪﮐ ﺎﺑ رد ﮏﺨﯿﻣ ﺲﻧﺎﺳا ﺞﯾﺎﺘﻧ ﻖﯿﻘﺤﺗ
ﺮﺿﺎﺣ
ﻣ ﺖﻘﺑﺎﻄ ﺖﺷاد ] 24 .[
تﺎﻘﯿﻘﺤﺗ ﺮﯾﺎﺳ ﻦﯿﻘﻘﺤﻣ ﺰﯿﻧ ﺮﺛا ﺖﻌﻧﺎﻤﻣ
ﯽﮔﺪﻨﻨﮐ ﺖﯿﻟﺎﻌﻓ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ رد
تﺎﺠﯾﺰﺒﺳ ﻒﻠﺘﺨﻣ
ﺖﺤﺗ
ﺮﯿﺛﺄﺗ ﺲﻧﺎﺳا يﺎﻫ ار ﯽﻫﺎﯿﮔ نﺎﺸﻧ ﯽﻣ ﺪﻫد ﻪﮐ ﺪﯾﻮﻣ ﺞﯾﺎﺘﻧ ﻖﯿﻘﺤﺗ
ﺮﺿﺎﺣ ﯽﻣ ﺪﻨﺷﺎﺑ ] 20 و 23 .[
Fig 1 Effect of a) Cumin b) fennel and c) Clove essential oils on peroxidase enzyme activity in courgette extract
Fig 2 3D surface of the simultaneous effect of different concentration of a) Cumin b) and fennel c) Clove essential oils and enyme activity time on peroxidase enzyme activity in courgette
يﺎﻬﺘﻧا ﻪﺑ ﻪﭼﺮﻫ ًﺎﺻﻮﺼﺧ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز تﺪﻣ ﺶﯾاﺰﻓا ﺎﺑ ﯽـﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ خﺮﻧ و تﺪﺷ ،ﻢﯾﻮﺷ ﮏﯾدﺰﻧ ﺶﻨﮐاو )
بﺬـﺟ (
ﯽﻣ ﺶﻫﺎﮐ ﯽﻣ ﻪﮐ ؛ﺪﺑﺎﯾ
ﺶﻫﺎـﮐ ،اﺮﺘﺴـﺑﻮﺳ ﺶﻫﺎـﮐ زا ﯽﺷﺎﻧ ﺪﻧاﻮﺗ
زا هﺪﻨﻨﮐ ﺖﻌﻧﺎﻤﻣ تﺎﺒﯿﮐﺮﺗ ﻞﯿﮑﺸﺗ ﺰﯿﻧ و ﻢﯾﺰﻧآ ﻮﯿﺗاﺪﯿﺴﮐا ﺖﯿﻟﺎﻌﻓ ﺪﺷﺎﺑ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ]
20 و 23 .[
ﺲﻧﺎﺳا ﺎﺑ ﺎﻫ ﺎـﺑ نﺪـﺷ ﺐﯿﮐﺮﺗ هﻮـﻬﻗ ﺶﻨﮐاو ﻊﻧﺎﻣ ﻂﯿﺤﻣ نﮋﯿﺴﮐا
رد و هﺪـﺷ ﯽـﻤﯾﺰﻧآ نﺪـﺷ يا
ﯽﻣ لﺮﺘﻨﮐ ار زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﺠﯿﺘﻧ ﺪـﻨﻨﮐ
] 24 .[
ﯽـﺋاراد
ﯽﻧﺎـﺧ ﻪـﻣﺮﮔ و
نارﺎـﮑﻤﻫ )
2010 ( ﺖـﯿﻟﺎﻌﻓ ﺶﯾاﺰـﻓا ﻢﯾﺰـﻧآ
ﻢﻠﮐ رد زاﺪﯿﺴﮐاﺮﭘ ﺪﯿﻔﺳ
و ﻢﻠﮐ ﺰﻣﺮﻗ ﺎﺑ ار ﺶﯾاﺰﻓا نﺎﻣز ﺖﯿﻟﺎﻌﻓ
و ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ﺲﻧﺎـﺳا
زار يﺎـﻫ ﺰﺒـﺳ هﺮـﯾز و ﻪـﻧﺎﯾ شراﺰـﮔ
ﺪﻧدﻮﻤﻧ ﯽﻟﺎﺣ رد ﻪـﮐ ﺎـﺑ ﺶﯾاﺰـﻓا ﺖـﻈﻠﻏ ﺮـﺛا ﮏـﺨﯿﻣ ﺲﻧﺎـﺳا
ﯽﮔﺪﻧرادزﺎﺑ ﺖﻓﺎﯾ ﺶﯾاﺰﻓا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ
ﯽﻣ ﻪﮐ ﺪﻧاﻮﺗ
ترﺪﻗ ﺖﻠﻋ ﻪﺑ ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ
و ﯽﮔﺪﻨﻨﮐ رﺎﻬﻣ
،نﮋﯿﺴﮐا ﺮﺗﻻﺎـﺑ
ﺲﻧﺎﺳا ﻪﺑ ﺖﺒﺴﻧ ﮏﺨﯿﻣ ﺲﻧﺎﺳا
ﺪـﺷﺎﺑ ﺰﺒـﺳ هﺮﯾز و ﻪﻧﺎﯾزار يﺎﻫ
] 26 .[
3 - 2 - ﻪﻨﯿﻬﺑ ﺎﺳ ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ يز
،ﺰﺒـﺳ هﺮـﯾز يﺎﻫ
ﻢﯾﺰـﻧآ يزﺎـﺳ لﺎـﻌﻓ ﺮـﯿﻏ ﺮـﺑ ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﺰﺒﺳ
لوﺪﺟ رد 2
و ﻞﻘﺘﺴﻣ يﺎﻫﺮﯿﻐﺘﻣ ﯽﻌﻗاو حﻮﻄﺳ ﺎﺑ ﯽﺸﯾﺎﻣزآ حﺮﻃ
زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺞﯾﺎﺘﻧ )
ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا بﺬﺟ (
ﻪﺑ طﻮﺑﺮﻣ
ﺮﯿﺛﺄﺗ ﺲﻧﺎﺳا يﺎﻫ ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،ﺰﺒﺳ هﺮﯾز ﻢﯾﺰـﻧآ ﺖـﯿﻟﺎﻌﻓ ﺮﺑ
اﺮﭘ وﺪﮐ زاﺪﯿﺴﮐ ﺰﺒﺳ
ﻂـﺳﻮﺗ هﺪـﺷ ﯽـﻨﯿﺑ ﺶﯿـﭘ ﺞﯾﺎـﺘﻧ هاﺮـﻤﻫ ﻪﺑ
تﻻدﺎﻌﻣ زا ﻞﺻﺎﺣ ﺖـﺳا هﺪـﺷ هداد نﺎﺸـﻧ ﺦﺳﺎﭘ ﺢﻄﺳ شور .
ﺲﻧﺎﯾراو ﻞﯿﻠﺤﺗ و ﻪﯾﺰﺠﺗ ﻪﺑ طﻮﺑﺮﻣ ﺞﯾﺎﺘﻧ )
ANOVA (
ﺖﯿﻟﺎﻌﻓ
ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﺖﺤﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ
ﻪﻧﺎﯾزار و هﺮﯾز ،ﮏﺨﯿﻣ يﺎﻫ
لوﺪﺟ رد 3
هﺪﺷ ﻪﺋارا ﺪﻧا . ﻣ ﻪﺑ ﻪﻧﺎﮔﺪﻨﭼ نﻮﯿﺳﺮﮔر ﺐﯾاﺮﺿ رﻮﻈﻨ
ﺶﯿﭘ لﺪﻣ ﯽﻨﯿﺑ ﺐﺳﺎﻨﻣ
ﺦﺳﺎﭘ ﺮﯿﻐﺘﻣ ياﺮﺑ ﺪﺷ ﻪﺒﺳﺎﺤﻣ
ﻪﺟﻮﺗ ﺎﺑ و
ﯽﻨﻌﻣ ﻪﺑ ﺐﯾاﺮﺿ يراد )
لوﺪﺟ 3 ( تﻻدﺎﻌﻣ ، 2
، 3 و 4 ﺐﯿﺗﺮﺗ ﻪﺑ
وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ياﺮﺑ ﺰﺒﺳ
ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﺖﺤﺗ يﺎـﻫ
ﺪﯾدﺮﮔ ﻪﺋارا ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،هﺮﯾز :
Y1= 0.468034 + (0.002333) × X1 + (0.303167)
× X2 - (0.03362) X1
2 – (0.09612) × X2 2 + (0.01275) × X1 X2 ( 2)
Y2= 0.432138 + (0.002167) × X1 + (0.2945) × X2- (0.00198) X12
– (0.08998) × X22
+ (0.007)
× X1 X2 (3)
Y3= 0.269138– (0.07367) × X1 + (0.207667) × X2 + (0.063517) X1
2 – (0.05748) × X2 2 – (0.054) × X1 X2 (4)
قﻮﻓ تﻻدﺎﻌﻣ رد ﻪﮐ X1
و X2
نﺎﻣز و ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﺐﯿﺗﺮﺗ ﻪﺑ
ﯽﻣ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺪﻨﺷﺎﺑ
.
ﻪﻟدﺎﻌﻣ 2 وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ تاﺮﯿﯿﻐﺗ ﺰﺒﺳ
ﺮﯿﺛﺄﺗ ﺖﺤﺗ
ﯽﻣ نﺎﺸﻧ ار هﺮﯾز ﺲﻧﺎﺳا ﺪﻫد
. نﺎﻤﻫ ﯽﻣ ﻪﻈﺣﻼﻣ ﻪﮐ رﻮﻃ ﻦﯾا دﻮﺷ
ترﻮﺻ ﻪﺑ ﻪﻟدﺎﻌﻣ ﺪﻨﭼ
ﻪﻠﻤﺟ يا و ﻪﺟرد مود ﯽﻣ ﺪﺷﺎﺑ . رد
ﺳا ﺮﯿﺛﺄﺗ ﯽﺳرﺮﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ هﺮﯾز ﺲﻧﺎ
وﺪﮐ ﺰﺒﺳ ،
نﻮﻣزآ ANOVA لﺪﻣ ﻪﮐ دﻮﻤﻧ ﺺﺨﺸﻣ
ﻪﺟرد مود هزاﺪﻧا ﻪﺑ ﺮﮕﻧﺎﯿﺑ ﯽﻓﺎﮐ
زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ )
ﺦﺳﺎﭘ ( ﺐﯾاﺮﺿ ﺎﺑ ،
ﯽﻣ ﺺﺨﺸﻣ ﺪﺷﺎﺑ
. ﺐﯾﺮﺿ ﻦﯿﯿﻌﺗ ﻻﺎﺑ 9997) /0
2 = ﺪﯾﻮﻣ(R
و هداد ﺢﯿﺿﻮﺗ ﯽﺑﻮﺧ ﻪﺑ ار ﺶﻨﮐاو ،نﻮﯿﺳﺮﮔر لﺪﻣ ﻪﮐ ﺖﺳا ﻦﯾا زاﺮﺑ لﺪﻣ ﻪﺘﺴﻧاﻮﺗ هﺪﺷ ش 97
/ 99 رد تاﺮﯿﯿﻐﺗ ﻞﮐ زا ﺪﺻرد
ﻪﻨﻣاد ﺪﻫد ﺢﯿﺿﻮﺗ ار ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﺮﯾدﺎﻘﻣ ي .
ﺮﯾدﺎﻘﻣ R2
و ﯽﻌﻗاو
R2
هﺪﺷ ﻞﯾﺪﻌﺗ ﺐﯿﺗﺮﺗ ﻪﺑ 2
9997 /0 و 9995 /0 ،ﺪﻧﺪﻣآ ﺖﺳد ﻪﺑ
هداد ﯽﮔﺪﻨﮐاﺮﭘ زا ﯽﺒﺳﺎﻨﻣ ﻒﯿﺻﻮﺗ ﺮﮕﻧﺎﯿﺑ ﺰﯿﻧ ﺪﻨﺘﺴﻫ ﺎﻫ
) ﻪﻟدﺎﻌﻣ
2 .(
نﻮﻣزآ ﻦﯿﻨﭽﻤﻫ ANOVA
ﺪﻨﭼ لﺪﻣ ﻪﮐ دﻮﻤﻧ ﺺﺨﺸﻣ
ﻪﻠﻤﺟ ﻪﺟرد يا مود ي ﻪﺑ ردﺎﻗ ﺮﺑ ﻪﻧﺎﯾزار ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﯽﻨﯿﺑ ﺶﯿﭘ
زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ وﺪﮐ
ﺰﺒﺳ ﯽﻣ ﺪﺷﺎﺑ . ﻪﻟدﺎﻌﻣ رد 3
ﺮﯾدﺎﻘﻣ ،
R2
و ﯽﻌﻗاو R2
ﺐﯿﺗﺮﺗ ﻪﺑ هﺪﺷ ﻞﯾﺪﻌﺗ 9998
/0 و 9997 /0 ﻪﺑ
هداد ﯽﮔﺪﻨﮐاﺮﭘ زا ﯽﺒﺳﺎﻨﻣ ﻒﯿﺻﻮﺗ ﺮﮕﻧﺎﯿﺑ ﻪﮐ ﺪﻧﺪﻣآ ﺖﺳد ﺎﻫ
ﯽﻣ ﺪﻨﺷﺎﺑ . شزاﺮﺑ ناﺪﻘﻓ نﻮﻣزآ زا هدﺎﻔﺘﺳا ﺎﺑ لﺪﻣ ندﻮﺑ ﺐﺳﺎﻨﻣ
3
ياﺮﺑ ﻪﮐ ﺖﻓﺮﮔ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ 05
/0
>
ﯽﻨﻌﻣ P دﻮﺒﻧ راد .
لﺪﻣ ﻪﻟدﺎﻌﻣ رد شزاﺮﺑ مﺪﻋ نﻮﻣزآ ضﺮﻓ )
ﻪﻟدﺎﻌﻣ 3 ( ﯽﻨﻌﻣ راد
دﻮﺒﻧ ) 05 /0
>
(P وﺪﮐ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ سﺎﺳا ﺮﺑ لﺪﻣ و
ﺰﺒﺳ ) ﻪﻧﺎﯾزار ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ ﺖﺤﺗ (
ﺪﯾدﺮﮔ شزاﺮﺑ .
ﺮﺑ بﻮﺧ شزا
تاﺮﯿﯿﻐﺗ ﻪﮐ ﺖﺳا ﻪﺘﺴﻧاﻮﺗ هﺪﺷ دﺎﺠﯾا لﺪﻣ ﻪﮐ ﺖﺳا ﯽﻨﻌﻣ ﻦﯾا ﻪﺑ هداد رد ﺪﻫد ﺢﯿﺿﻮﺗ ﯽﻓﺎﮐ هزاﺪﻧا ﻪﺑ ار ﺎﻫ ]
20 [ لﺪﻣ ﻦﯾا اﺬﻟ ،
ﺶﯿﭘ ﺖﻬﺟ دﻮﺑ ﺐﺳﺎﻨﻣ هدﺎﻔﺘﺳا درﻮﻣ يﺎﻫﺮﯿﻐﺘﻣ ﻪﻨﻣاد رد ﯽﻨﯿﺑ
) ﻪﻟدﺎﻌﻣ 3 .(
ﺮﯾدﺎﻘﻣ ﻦﯿﻨﭽﻤﻫ R2
و ﯽﻌﻗاو R2
رد هﺪﺷ ﻞﯾﺪﻌﺗ
ﻪﻟدﺎﻌﻣ 4 ﺐﯿﺗﺮﺗ ﻪﺑ ، 9713
/0 و 9503 /0 ﯽﻣ ﺪﺷﺎﺑ ﺮﮕﻧﺎﯿﺑ ﻪﮐ
هداد ﯽﮔﺪﻨﮐاﺮﭘ ﺐﺳﺎﻨﻣ ﻒﯿﺻﻮﺗ ﻪﻟدﺎﻌﻣ ﻂﺳﻮﺗ ﺎﻫ
4 ﯽﻣ ﺪﻨﺷﺎﺑ .
ﻞﮑﺷ ) 3 ( زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﯽﻌﻗاو ﺮﯾدﺎﻘﻣ ﻦﯿﺑ ﻪﻄﺑار
ﻂﺳﻮﺗ هﺪﺷ ﯽﻨﯿﺑ ﺶﯿﭘ ﺮﯾدﺎﻘﻣ و ﺶﯾﺎﻣزآ زا ﻞﺻﺎﺣ ﻪﻟدﺎﻌﻣ
يﺎﻫ
،Y1 و Y2 ياﺮﺑ Y3 ﺲﻧﺎﺳا يﺎﻫ ار ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،هﺮﯾز
نﺎﺸﻧ ﯽﻣ ﺪﻫد . نﺎﻤﻫ ﻃ رﻮ ﻞﮑﺷ رد ﻪﮐ )
3 ( هﺪﻫﺎﺸﻣ ﯽﻣ دﻮﺷ
ﺐﯾﺮﺿ ﺮﯾدﺎﻘﻣ ﻦﯿﯿﻌﺗ
2) (R ﻦﯿﺑ ﯽﯾﻻﺎﺑ هداد
يﺎﻫ ﺶﯿﭘ و ﯽﻌﻗاو
ﺖﺤﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ياﺮﺑ لﺪﻣ ﻂﺳﻮﺗ هﺪﺷ ﯽﻨﯿﺑ ﺮﯿﺛﺄﺗ
هﺪﻫﺎﺸﻣ ﺲﻧﺎﺳا ﻪﺳ ﺮﻫ ﯽﻣ
دﻮﺷ يﻻﺎﺑ ﺖﻗد هﺪﻨﻫد نﺎﺸﻧ ﻪﮐ
ﻂﺑاور ،Y1 و Y2 وY3 لﺪﻣ يﺎﻫ رد هﺪﺷ دﺎﺠﯾا ﺦﺳﺎﭘ ﺢﻄﺳ
ﻖﯿﻘﺤﺗ ﻦﯾا ﯽﻣ
ﺑ ﺪﺷﺎ .
2. Adjusted R-Squared
Table 2 Central composite design, actual levels of independent variables and peroxidase activity of crude extracts of courgette under effect of different essential oils.
Predicted dependent variable (Response) Actual dependent variable
(Response) Independent variables
Enzyme activity or Absorbance Enzyme activity or Absorbance
Clove essential
oil Fennel
essential oil Cumin
essential oil Clove
essential oil Fennel
essential oil Cumin
essential oil The time
of enzyme Activity (X2) (s) Essential oil
Concentration (X1) (ppm) Treatment
number
0.087 0.051
0.046 0.049
0.049 0.049
200 100
1
0.048 0.041
0.025 0.020
0.040 0.03
400 0
2
0.611 0.626
0.626 0.623
0.623 0.623
0 0
3
0.355 0.644
0.657 0.378
0.642 0.655
0 100
4
0.406 0.428
0.432 0.432
0.432 0.432
0 200
5
0.259 0.432
0.437 0.264
0.435 0.433
200 100
6
0.004 0.048
0.069 0.070
0.050 0.06
200 100
7
0.419 0.637
0.675 0.384
0.641 0.68
200 200
8
0.269 0.432
0.468 0.262
0.435 0.471
200 100
9
0.269 0.432
0.468 0.265
0.430 0.469
400 100
10
0.269 0.432
0.468 0.263
0.432 0.469
400 200
11
0.269 0.432
0.468 0.264
0.429 0.468
200 100
12
0.269 0.432
0.468 0.261
0.428 0.467
200 0
13
Table 3 Analysis variance of regression coefficients of predicted quadratic polynomial models for predicting peroxidase enzyme activity in courgette under effect of different essential oils.
Essential
oil Source DF Sum of
squares
Mean of
squares F-Value p-Value Coefficient Model 2 0.593599 0.11872 4611.511 < 0.0001 0.468034
X1 1 3.27×10-5 3.27×10-5 1.268892 0.2971 0.002333
X2 1 0.55146 0.55146 21420.71 < 0.0001 0.303167
X1 × X1 0.003122 0.003122 121.2667 < 0.0001 -0.03362
X2 × X2 0.025518 0.025518 991.202 < 0.0001 -0.09612
X1 × X2 0.00065 0.00065 25.25806 0.0015 0.01275
Residual 10 0.00018 2.57×10-5 - - -
Lack of fit 6 0.000171 5.71×10-5 25.97118 0.0044 -
Pure error 4 8.8×10-6 2.2×10-6 - - -
Cumin
Total 12 0.59378 - - - -
Model 5 0.547217 0.109443 7659.274 < 0.0001 0.432138
X1 1 2.82×10-5 2.82 ×10-5 1.971214 0.2031 0.002167
X2 1 0.520382 0.520382 36418.33 < 0.0001 0.2945
X1 × X1 1 1.09×10-5 1.09 ×10-5 0.759883 0.4123 -0.00198 X2 × X2 1 0.022363 0.022363 1565.04 < 0.0001 -0.08998
X1 × X2 1 0.000196 0.000196 13.71685 0.0076 0.007
Residual 7 0.0001 1.43 ×10-5 - - -
Lack of fit 3 6.92×10-5 2.31×10-5 2.996666 0.1583 -
Pure error 4 3.08×10-5 7.7 ×10-6 - - -
Fennel
Total 12 0.547317 - - - -
Model 5 0.3177 0.06354 47.33027 < 0.0001 0.269138
X1 1 0.032561 0.032561 24.25413 0.0017 -0.07367
X2 1 0.258753 0.258753 192.7424 < 0.0001 0.207667
X1 × X1 1 0.011143 0.011143 8.300119 0.0236 0.063517
X2 × X2 1 0.009126 0.009126 6.797923 0.0351 -0.05748
X1 × X2 1 0.011664 0.011664 8.688401 0.0215 -0.054
Residual 7 0.009397 0.001342 - - -
Lack of fit 3 0.009387 0.003129 1251.648 < 0.0001
Pure error 4 0.00001 2.5 ×10-6 - - -
Clove
Total 12 0.327097 - - - -
** significant at 1%, * significant at 5%, ns non significant
Fig 3 The actual versus predicted value for peroxidase enzyme activity under effect of a)
cumin b) fennel and c) clove essential oil ﻞﮑﺷ 4 ﻞﻘﺘﺴﻣ يﺎﻫﺮﯿﻐﺘﻣ ياﺮﺑ هﺪﺷ ﻦﯿﯿﻌﺗ ﻂﯾاﺮﺷ )
ﻪﻨﯿﻬﺑ ﺖﻬﺟ
ﺲﻧﺎﺳا ﺮﯿﺛﺄﺗ يزﺎﺳ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﻒﻠﺘﺨﻣ يﺎﻫ
وﺪﮐ
ﺰﺒﺳ ( ﯽﻣ نﺎﺸﻧ ار هﺪﺷ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ و ﺪﻫد
. ﻞﮑﺷ ﻦﯾا رد ﺎﻫ
ﻞﻘﺘﺴﻣ يﺎﻫﺮﯿﻐﺘﻣ )
ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و ﺲﻧﺎﺳا ﺖﻈﻠﻏ (
رد
ﺪﺷ مﺎﺠﻧا تﺎﺸﯾﺎﻣزآ هدوﺪﺤﻣ رد ﺖﺳا هﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ه
ﻞﻗاﺪﺣ ،ﺪﻨﯾآﺮﻓ فﺪﻫ ناﻮﻨﻋ ﻪﺑ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﻪﮑﯿﻟﺎﺣ ﺖﺳا هﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد .
ﻪﻨﯿﻬﺑ ﺪﻨﯾآﺮﻓ رد ﯽﻣﺎﻤﺗ ﻪﺑ يزﺎﺳ
ﺪﺷ هداد نﺎﺴﮑﯾ ﺖﯿﻤﻫا و نزو ﻞﻘﺘﺴﻣ يﺎﻫﺮﺘﻣارﺎﭘ .
ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﻞﺣ هار ﺮﻈﻧ درﻮﻣ ﻂﯾاﺮﺷ ﺶﯿﭘ يﺎﻫ
سﺎﺳا ﺮﺑ ﻪﮐ هﺪﺷ ﯽﻨﯿﺑ ﺖﯿﺑﻮﻠﻄﻣ ﻦﯾﺮﺗﻻﺎﺑ
ﻞﮑﺷ رد ﺲﻧﺎﺳا ﺮﻫ ياﺮﺑ 4
ﺖﺳا هﺪﺷ ﻪﺋارا
ﻪﺑ ﺖﯿﺑﻮﻠﻄﻣ ﻪﭼﺮﻫ و 1
ﮏﯾدﺰﻧ ﺐﺳﺎﻨﻣ ﺪﺷﺎﺑ ﺮﺗ ﻦﯾﺮﺘﻬﺑ و ﻦﯾﺮﺗ
ﻂﯾاﺮﺷ ﻦﯾﺮﺘﻬﺑ ناﻮﻨﻋ ﻪﺑ لوا ﻞﺣ هار ﻪﮐ دﻮﺑ ﺪﻫاﻮﺧ ﻂﯾاﺮﺷ ﺖﺳد ﺖﻬﺟ لﺎﻤﻋا ﺎﺑ و ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻪﺑ ﯽﺑﺎﯾ
ﻢﮐ يزﺎﺳ ﻪﻨﯿﻬﺑ رد هﺪﻣآ ﺖﺳﺪﺑ ﺪﻨﯾآﺮﻓ ﻂﯾاﺮﺷ راﺪﻘﻣ ﻦﯾﺮﺗ
ﺖﯿﻟﺎﻌﻓ ﺲﻧﺎﺳا زا ماﺪﮐ ﺮﻫ ﺮﯿﺛﺄﺗ ﺖﺤﺗ ﯽﻤﯾﺰﻧآ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
ﺪﻣآ ﺪﻫاﻮﺧ ﺖﺳﺪﺑ .
نﺎﻤﻫ رﻮﻃ ﻞﮑﺷ رد ﻪﮐ 4
) و A1 (A2
هﺪﻫﺎﺸﻣ ﯽﻣ دﻮﺷ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ياﺮﺑ ﻂﯾاﺮﺷ ﻦﯾﺮﺘﻬﺑ
ﺖﺤﺗ زاﺪﯿﺴﮐاﺮﭘ ﺮﯿﺛﺄﺗ
ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﻞﻣﺎﺷ هﺮﯾز ﺲﻧﺎﺳا
5/ppm 194 ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و 02
/0 ﻪﯿﻧﺎﺛ ﯽﻣ ﺪﺷﺎﺑ رد
ﻪﮑﯿﻟﺎﺣ ﻪﺑ ﯽﺑﺎﯾ ﺖﺳد ياﺮﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻢﮐ
ﻦﯾﺮﺗ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ
ﻪﻧﺎﯾزار ﺲﻧﺎﺳا ﺖﻈﻠﻏ زا هدﺎﻔﺘﺳا ﻞﻣﺎﺷ زاﺪﯿﺴﮐاﺮﭘ 200 ppm
و
ﺲﻧﺎﺳا ﺖﻈﻠﻏ زا هدﺎﻔﺘﺳا و ،ﻪﯿﻧﺎﺛ ﺮﻔﺻ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز ﮏﺨﯿﻣ ppm 200 ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و 7/
1 ﻪﯿﻧﺎﺛ ﯽﻣ ﺪﺷﺎﺑ .
ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ لﺎﻤﻋا ﺎﺑ و
هدﺎﻔﺘﺳا زا ﺖﻈﻠﻏ يﺎﻫ ﻪﻨﯿﻬﺑ ﺲﻧﺎﺳا يﺎﻫ
و هدﻮﺑ ﻞﻗاﺪﺣ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،هﺮﯾز اﺮﺑ ﺐﯿﺗﺮﺗ ﻪﺑ ﺮﺑ
029 /0 ، 041 /0 و 013 /0 ﯽﻣ ﺪﺷﺎﺑ .
3 - 3 - ﯽﻠﮐ يﺮﯿﮔ ﻪﺠﯿﺘﻧ
هﻮﻬﻗ ﯽﻤﯾﺰﻧآ نﺪﺷ يا هﻮـﯿﻣ رد
ﺮـﺛا رد ﺐـﻠﻏا تﺎﺠﯾﺰﺒـﺳ و ﺎـﻫ
ﻪـﮐ هﺪـﺷ مﺎﺠﻧا زاﺪﯿﺴﮐاﺮﭘ و زاﺪﯿﺴﮐا ﻞﻨﻓ ﯽﻠﭘ ﻢﯾﺰﻧآ ود ﺖﯿﻟﺎﻌﻓ ﯿﯿﻐﺗ ﺐﺒﺳ بﻮﻠﻄﻣﺎﻧ ﯽﻔﯿﮐ تاﺮ ﻪﯾﺬﻐﺗ شزرا و ﻢﻌﻃ ،ﮓﻧر رد
يا
ﺎﺠﺑﺎﺟ ،يﺪﻨﺑ ﻪﺘﺴﺑ ﻦﯿﺣ رد ﯾ
يرادرﺎﺒﻧا و ﯽ ﯽﻣ
دﻮﺷ . ﻪـﺑ ﻪﺟﻮﺗ ﺎﺑ
و ﻪﺘﺷاد ﻢﯾﺰﻧآ ود ﻦﯾا ﻞﻤﻋ ﺖﯿﻫﺎﻣ رد ﯽﻤﻬﻣ ﺶﻘﻧ نﮋﯿﺴﮐا ﻪﮑﻨﯾا نﻮﯿﺳاﺪﯿﺴﮐا ﺶﻨﮐاو ﯽﻋﻮﻧ ًﺎﻣﻮﻤﻋ -
ﯽـﻣ بﻮﺴـﺤﻣ ﺎـﯿﺣا ،ﺪﻧﻮـﺷ
ﯽﻣ ناﺪﯿﺴﮐا ﯽﺘﻧآ تﺎﺒﯿﮐﺮﺗ زا هدﺎﻔﺘﺳا اﻮﻨﻋ ﻪﺑ ﺪﻧاﻮﺗ
ياﺮﺑ ﯽﻠﻣﺎﻋ ن
ﻢﯾﺰﻧآ ﻦﯾا ﺖﯿﻟﺎﻌﻓ رﺎﻬﻣ زا ﻖﯿﻘﺤﺗ ﻦﯾا رد ﻦﯾاﺮﺑﺎﻨﺑ ؛ﺪﺷﺎﺑ حﺮﻄﻣ ﺎﻫ
ﺲﻧﺎﺳا ﯽﻫﺎﯿﮔ يﺎﻫ )
ﮏﺨﯿﻣ و ﻪﻧﺎﯾزار ،هﺮﯾز (
صاﻮـﺧ ياراد ﻪﮐ
يﺎـﻫ ناﺪﯿﺴـﮐا ﯽـﺘﻧآ ناﻮﻨﻋ ﻪﺑ ﺪﻨﺘﺴﻫ هﺪﺷ تﺎﺒﺛا ﯽﺑوﺮﮑﯿﻣ ﺪﺿ ﺎـﺗ ﺪـﺷ هدﺎﻔﺘـﺳا زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ يزﺎﺳ لﺎﻌﻓ ﺮﯿﻏ ياﺮﺑ ﯽﻌﯿﺒﻃ ﺧ زا هدﺎﻔﺘﺳا ﺮﺑ هوﻼﻋ نآ ﺖﯿﻠﺑﺎﻗ ﻦﯾا زا ﯽﺑوﺮﮑﯿﻣ ﺪﺿ ﺖﯿﺻﺎ
ﺎـﻫ
دﻮﺷ هدﺎﻔﺘﺳا ﺰﯿﻧ .
ﺺﻟﺎﺧ مﺮﻓ ﺎﻬﻨﺗ ﻪﮐ داد نﺎﺸﻧ ﻖﯿﻘﺤﺗ ﻦﯾا ﺞﯾﺎﺘﻧ
ﺖﻈﻠﻏ و 200ppm
و ﺺﻟﺎﺧ مﺮﻓ ﻦﯿﻨﭽﻤﻫ و ﺰﺒﺳ هﺮﯾز ﺲﻧﺎﺳا
ﺖﻈﻠﻏ يﺎﻫ يﻻﺎﺑ ) 100 و 200ppm ( ﯽﯾﺎـﻧاﻮﺗ ،ﻪﻧﺎﯾزار ﺲﻧﺎﺳا
وﺪـﮐ زاﺪﯿﺴـﮐاﺮﭘ ﻢﯾﺰـﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺰﺒـﺳ
ﺎـﻣا ﺪـﻧدﻮﺑ اراد ار
ﺘﺳا زا هدﺎﻔ ﺖﻈﻠﻏ يﺎﻫ 50 و 75ppm ﺚـﻋﺎﺑ ﻪـﻧﺎﯾزار ﺲﻧﺎـﺳا
ﺖﺳا هﺪﺷ ﺰﯿﻧ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﯾاﺰﻓا .
ﮏـﺨﯿﻣ ﺲﻧﺎﺳا
ﻪﯿﻠﮐ رد ﺖﻈﻠﻏ يﺎﻫ ﻢﯾﺰـﻧآ ﺖﯿﻟﺎﻌﻓ ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﻪﻌﻟﺎﻄﻣ درﻮﻣ
ﺪﺷ زاﺪﯿﺴﮐاﺮﭘ ﻪﮐ
ﺖﻠﻋ ﻪﺑ ياﺮﺘﺴـﺑﻮﺳ ﺶﻫﺎـﮐ ﯽﻓﺮﺼـﻣ
و ﺎـﯾ
ﺶﻫﺎﮐ نﮋﯿﺴﮐا درﻮﻣ زﺎـﯿﻧ ﻢﯾﺰـﻧآ دﺎـﺠﯾا ياﺮـﺑ ﮓـﻧر
هﻮـﻬﻗ يا
ﯽﻣ ﺪﺷﺎﺑ نﺎﺸﻧ ﺞﯾﺎﺘﻧ ﻦﯾا ﻪﮐ هﺪﻨﻫد
ﯽﯾﺎﻧاﻮﺗ رد ﮏـﺨﯿﻣ ﺲﻧﺎـﺳا
ﺶﻨﮐاو ﺎﺑ نﮋﯿﺴﮐا ﻂﯿﺤﻣ و ﺖﻌﻧﺎﻤﻣ زا ﺶﻨﮐاو زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ
و ﯽﻠﭘ لﻮﻨﻓ زاﺪﯿﺴﮐا ) هﻮﻬﻗ يا نﺪﺷ ﯽـﻤﯾﺰﻧآ ( ﯽـﻣ ﺪـﺷﺎﺑ . ﺞﯾﺎـﺘﻧ
يزﺎـﺳ لﺎـﻌﻓ ﺮﯿﻏ ياﺮﺑ ﻂﯾاﺮﺷ ﻦﯾﺮﺘﻬﺑ ﻪﮐ داد نﺎﺸﻧ يزﺎﺳ ﻪﻨﯿﻬﺑ ﺖﺤﺗ زاﺪﯿﺴﮐاﺮﭘ ﻢﯾﺰﻧآ
ﺮﯿﺛﺄﺗ ﻣﺎﺷ هﺮﯾز ﺲﻧﺎﺳا ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﻞ
5/ppm 194 ﯽـﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و 02
/0 ﻪـﯿﻧﺎﺛ ﯽـﻣ ﺪـﺷﺎﺑ رد
ﻪﺑ ﯽﺑﺎﯾ ﺖﺳد ياﺮﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻪﮑﯿﻟﺎﺣ ﻢﮐ
ﻦﯾﺮﺗ ﻢﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ
زا هدﺎﻔﺘﺳا ﻞﻣﺎﺷ زاﺪﯿﺴﮐاﺮﭘ 200ppm
ﻪﻧﺎﯾزار ﺲﻧﺎﺳا نﺎـﻣز و
زا هدﺎﻔﺘـﺳا و ،ﻪـﯿﻧﺎﺛ ﺮﻔـﺻ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ 200ppm
ﺲﻧﺎـﺳا
ﮏﺨﯿﻣ ﯽﻤﯾﺰﻧآ ﺖﯿﻟﺎﻌﻓ نﺎﻣز و 7
/1 ﻪﯿﻧﺎﺛ ﯽﻣ ﺪﺷﺎﺑ .
Fig 4 The optimum conditions for inactivation of peroxidase enzyme activity in courgette under simultaneous effect of different concentration of A) Cumin B) fennel and
C) clove essential oils and enzyme activity time
4 - ﺑﺎﻨﻣ ﻊ
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