ﻪﻠﺠﻣ مﻮﻠﻋ يزار ﻲﻜﺷﺰﭘ هرود
18
، هرﺎﻤﺷ 91
، يد ﻲﺸﻫوﮋﭘ ﻪﻟﺎﻘﻣ 1390
ﻪﻣﺪﻘﻣ
رد تادﻮﺟﻮﻣ ﺮﭘ
ﻲﻟﻮﻠﺳ ﻪﻛ لﻮﻠﺳ ﺎـﻫ ﻲﻳﺎـﻧاﻮﺗ ﻢﻴـﺴﻘﺗ
،ﺪﻧراد يﺮﻴﮔﻮﻠﺟ زا
ﺮﻴﺜﻜﺗ ﻲـﺑ ﻪـﻳور نآ ﺎـﻫ رﺎﻴـﺴﺑ ﺰﺋﺎـﺣ
ﺖﻴﻤﻫا ﺖﺳا . ﻪﺑ مﺎﮕﻨﻫ زوﺮـﺑ ﻚـﻳ ﺶﻨـﺗ ﺮـﻴﻈﻧ رﻮـﻀﺣ
يﺎــﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮــﻳ
ﻪــﻛ رد DNAنآ رﺎــﭼد ﻪﻣﺪــﺻ
ﻲﻣ
،ددﺮﮔ يﺎﻫ نژ ﺮﮕﺑﻮﻛﺮـﺳ
رﻮـﻣﻮﺗ ﺮـﻴﻈﻧ
،p53 لﺎـﻌﻓ
هﺪﺷ و ناﺰﻴﻣ نﺎﻴﺑ نآ ﺶﻳاﺰﻓا ﻲﻣ ﺪﺑﺎﻳ .
ﻦﻴﺌﺗوﺮــﭘ ﻪــﻛp53
ﻦﻴﺌﺗوﺮــﭘ يرﻮــﻣﻮﺗ
Tp53 ) ( ﺰــﻴﻧ
هﺪﻴﻣﺎﻧ ﻲﻣ
،دﻮﺷ ﻢﻴـﻈﻨﺗ هﺪـﻨﻨﻛ ﻪـﺧﺮﭼ ﻲﻟﻮﻠـﺳ هدﻮـﺑ و
ﻲﻣ ناﻮﺗ نآ ار ﻒـﻗﻮﺘﻣ هﺪـﻨﻨﻛ رﻮـﻣﻮﺗ ﻢـﻫ ﺪـﻴﻣﺎﻧ ) 1 و 2 .(
ناﺰﻴﻣ رد p53 لﻮﻠﺳ يﺎﻫ ﻲﻌﻴﺒﻃ ﻢﻛ و ﻪﺑ رﻮﻃ ﻪﺘـﺳﻮﻴﭘ
p53 يزﺎﺳزﺎﺑ
ارDNA ﻢﻴﻈﻨﺗ ﻲﻣ
،ﺪﻨﻨﻛ ﻞﺼﺘﻣ هﺪﺷ و گﺮﻣ
ﻪﻣﺎﻧﺮﺑ يﺰﻳر هﺪﺷ لﻮﻠﺳ ار لﺮﺘﻨﻛ ﻲﻣ ﺪﻨﻛ ) 6 - 3 .(
ﺎﻣا ﻞﻜﺸﻣ ﺖﺳﺎﺠﻨﻳا ﻪﻛ
رد لﻮﻠﺳ يﺎﻫ
،ﻲﻧﺎﻃﺮﺳ دﻮـﺧ
p53نژ ﺰــﻴﻧ ﻦــﻜﻤﻣ ﺖــﺳا ﺮــﻴﻴﻐﺗ ﻪــﺘﻓﺎﻳ و ﺮــﻴﻏ لﺎــﻌﻓ
ددﺮﮔ ) 7 و 8 .(
تﺎﻘﻴﻘﺤﺗ نﺎـﺸﻧ
ﻲـﻣ ﺪـﻨﻫد ﻪـﻛ
ًادوﺪـﺣ رد
% 50 زا ﻲﻣﺎﻤﺗ نﺎﻃﺮﺳ p53ﺎﻫ
ﺮﻴﻴﻐﺗ اﺪـﻴﭘ هدﺮـﻛ ﺖـﺳا
) 11 - 9 (.
نﺎﺼﺼﺨﺘﻣ ﻚـﻴﺘﻧژ
ﺪـﻧﺪﻘﺘﻌﻣ ﺮـﮔا
p53نژ رد
لﻮﻠﺳ يﺎﻫ ﻲﻧﺎﻃﺮﺳ لﺎﻌﻓ
،دﻮـﺷ ﺪـﺷر ﻦـﻳا لﻮﻠـﺳ ﺎـﻫ
ﻒﻗﻮﺘﻣ ﻲـﻣ ددﺮـﮔ . ﻦـﻳا
،ﺮـﻴﻴﻐﺗ ﺖـﺻﺮﻓ ﻲﺒـﺳﺎﻨﻣ ياﺮـﺑ
ﻢﺘﺴﻴﺳ ﻲﻨﻤﻳا ﻢﻫاﺮﻓ ﻲﻣ دروآ ﺎﺗ لﻮﻠﺳ يﺎﻫ ﻲﻧﺎﻃﺮـﺳ ار
زا ﻦﻴﺑ دﺮﺒﺑ ) 12 و 13 .(
DNA
* ﻼﻬﺷ ﺮﺘﻛد يﺪﻤﺤﻣ : رﺎﻳدﺎﺘﺳا ﺺﺼﺨﺘﻣ و ﻚﻳﺰﻴﻓﻮﻴﺑ هﺪﻜﺸﻫوﮋﭘ ،ﻲﻟﻮﻜﻟﻮﻣ مﻮﻠﻋ
ﻪﺘﺴﻫ هﺎﮕﺸﻫوﮋﭘ ،يا مﻮﻠﻋ
و نﻮﻨﻓ ﻪـﺘﺴﻫ ناﺮـﻬﺗ ،يا - ناﺮﻳا
*) لوﻮﺌﺴﻣ ﻒﻟﻮﻣ (.
ﺪﻤﺤﻣ ﺎﺿر ﻲﺘﺋاﺮﻗ : هرود يﻮﺠﺸﻧاد ﻲﺼﺼﺨﺗ يﺮﺘﻛد
هﺪﻜﺸﻧاد ،ﻲﻤﻴﺷﻮﻴﺑ هﺎﮕﺸﻧاد ،مﻮﻠﻋ
ﺖﻴﺑﺮﺗ
،سرﺪﻣ ناﺮﻳا ،ناﺮﻬﺗ [email protected].
يﺮﺒﻛ ﻲﻛز نﺎﺧ - ﮓﻨﻟا هرود يﻮﺠﺸﻧاد : ﻲﻜﺷﺰﭘ ﻲﺼﺼﺨﺗ يﺮﺘﻛد
هﺪﻜﺸﻧاد ،ﻲﻟﻮﻜﻟﻮﻣ مﻮﻠﻋ
ﻪﻳﺎﭘ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ مﻮﻠﻋ
ﻲﻜﺷﺰﭘ ناﺮﻳا ،ﻦﻳوﺰﻗ ،ﻦﻳوﺰﻗ .
[email protected] ﺎﺳر ﻲﻳﺎﺟر ﻲﺳﺎﻨﺷرﺎﻛ: ﺪﺷرا ﺖﺴﻳز ﻲﻟﻮﻠﺳ - هﺪﻜﺸﻫوﮋﭘ ،ﻲﻟﻮﻜﻟﻮﻣ دﺮﺑرﺎﻛ
هﺎﮕﺸﻫوﮋﭘ ،ﺎﻫﻮﺗﺮﭘ مﻮﻠﻋ
و نﻮﻨﻓ ﻪـﺘﺴﻫ
،يا ناﺮﻳا ،ناﺮـﻬﺗ [email protected] .
ﻪﺴﻳﺎﻘﻣ زوﺮﺑ
ﻦﻴﺌﺗوﺮﭘ رد
p53هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ﺎﺑ
هوﺮﮔ ﺪﻫﺎﺷ
هﺪﻴﻜﭼ فﺪﻫ و ﻪﻨﻴﻣز :
يﺎﻫ ﺶﻨﺗ نﻮﮔﺎﻧﻮﮔ ﺮﻴﻈﻧ يﺎﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮﻳ ﻲﻣ ﺪﻨﻧاﻮﺗ ﺐﺟﻮﻣ ﺶﻳاﺰﻓا يﺎﻫ ﺐﻴﺳآ ﻲﻟﻮﻠﺳ صﻮﺼﺧ ﻪﺑ رد ﺶﺨﺑ ﻪﺘـﺴﻫ نآ ﻲـﻨﻌﻳ DNA
ﺪﻧدﺮﮔ . ياﺮﺑ ﺖﻈﻓﺎﺤﻣ زا ﻦﻳا ﻪﻧﻮﮔ
،ﺎﻫ ﺐﻴﺳآ نژ يﺎﻫ ﻲﻤﻴﻈﻨﺗ ﻲﻌﻴﺒﻃ ﺪﻨﻧﺎﻣ نژ بﻮﻛﺮﺳ هﺪﻨﻨﻛ رﻮﻣﻮﺗ Tp53 دراو ﻞﻤﻋ ﻲﻣ ﺪﻧدﺮﮔ . ﻦﻳارد
،طﺎﺒﺗرا رد ﻦﻴﺑ
ﻦﻴﻠﻏﺎﺷ يرﺎﻛﻮﺗﺮﭘ ﻪﻛ هراﻮﻤﻫ ﺎﺑ يﺎﻫزود ﻲﻨﻴﻳﺎﭘ زا يﺎﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮﻳ رﺎﻛوﺮﺳ ﺪﻧراد ﻦﻳا،
نژ ﻲﺳرﺮﺑ و ناﺰﻴﻣ زوﺮﺑ نآ ﺎﺑ هوﺮﮔ لﺮﺘﻨﻛ ﻪﺴﻳﺎﻘﻣ ﺪﺷ .
شور رﺎﻛ : ﺎﺑ ،ﻲﻠﻴﻠﺤﺗ ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد هدﺎﻔﺘﺳا
زا ﺖﻴﻛ
،اﺰﻳﻻا راﺪﻘﻣ ﻦﻴﺌﺗوﺮﭘ مﺮﺳ p53
ﻲﻧﻮﺧ رد 42 و رﺎـﻛﻮﺗﺮﭘ دﺮﻓ 16
هزاﺪـﻧا ﺪﻫﺎـﺷ دﺮـﻓ يﺮـﻴﮔ
ﺪـﺷ .
ﻲﺳرﺮﺑ يﺎﻫ يرﺎﻣآ ﻂﺳﻮﺗ student t-testشور
مﺎﺠﻧا ﺖﻓﺮﮔ . ﻪﺑ ناﻮﻨﻋ هوﺮﮔ رﺎﻛﻮﺗﺮﭘ زا ﻦﻴﻠﻏﺎﺷ نﺎﻣزﺎﺳ يژﺮﻧا ﻲـﻤﺗا ناﺮـﻳا ) ﺎـﺑ ﻦﻴﮕﻧﺎـﻴﻣ ﻲﻨـﺳ 3 /
±1
8 / 37 لﺎﺳ ( تﻮﻋد ﺪﺷ يرﺎﻜﻤﻫ ﻪﺑ .
ﻪﺑ ناﻮﻨﻋ هوﺮﮔ ﺪﻫﺎﺷ ﺰﻴﻧ ﺮﻈﻧ زا ﻪﻛ ﻲﻨﻴﺒﻠﻃواد
،ﻦﺳ ﺲﻨﺟ و تادﺎﻋ رﺎﮕﻴﺳ نﺪﻴﺸﻛ ﺎﺑ هوﺮﮔ رﺎﻛﻮﺗﺮﭘ ﻖﺑﺎﻄﺗ ﺪﻨﺘﺷاد ) ﺎـﺑ
ﻦﻴﮕﻧﺎﻴﻣ ﻲﻨﺳ /
±2 5 / 34 لﺎﺳ (
، ﺪﻧﺪﻳدﺮﮔ بﺎﺨﺘﻧا .
زا 2 ﻲﺳ ﻲﺳ ﺪﺷ جاﺮﺨﺘﺳا ژﻮﻔﻳﺮﺘﻧﺎﺳ ﻂﺳﻮﺗ نآ مﺮﺳ ،دﺮﻓ ﺮﻫ ﻪﻨﻳرﺎﭙﻫ نﻮﺧ .
ﻪﺘﻓﺎﻳ ﺎﻫ نژ نﺎﻴﺑ: ﺑ رﺎﻛ ﻮﺗﺮﭘ هوﺮﮔ ردp53
ﻪ ﻲﻨﻌﻣ رﻮﻃ ﺪﻫﺎﺷ هوﺮﮔ زا ﺮﺗﻻﺎﺑ يراد دﻮﺑ
) 0225 / 0 p= .(
هوﻼﻋ ﺮﺑ
،ﻦﻳا ﻪﻘﺑﺎﺳ رﺎﻛ رﺎﻛﻮﺗﺮﭘ و ﺎﻳ فﺮـﺼﻣ رﺎﮕﻴـﺳ
يﺮﻴﺛﺎﺗ ﺮﺑ ناﺰﻴﻣ نﺎﻴﺑ ﻦﻳا نژ ﻪﺘﺷاﺪﻧ ﺖﺳا .
ﻪﺠﻴﺘﻧ يﺮﻴﮔ : يﺎﻫزود ﺮﻤﺘﺴﻣ و ﻦﻴﻳﺎﭘ يﺎﻫﻮﺗﺮﭘ
ناﺰﻴﻧﻮﻳ رد ﻲﻳاﺰﺳ ﻪﺑ ﺶﻘﻧ ﺶﻳاﺰﻓا
نﺎﻴﺑ p53نژ رد ﻦﻴﺑ ﻦﻴﻠﻏﺎﺷ رﺎﻛﻮﺗﺮﭘ ﺖﺳا ﻪﺘﺷاد . رد ﻦـﻳا و ﻲﻟﺎـﺣ
ﻪﻛ ﺖﺳا ﺮﺑ سﺎﺳا ﺞﻳﺎﺘﻧ ﺖﺒﺛ هﺪﺷ رد يﺮﺘﻤﻳزود يدﺮﻓ ﻦﻳا
،داﺮﻓا زود ﻲﺘﻓﺎﻳرد رد هدوﺪﺤﻣ زود زﺎﺠﻣ ﻪﻧﻻﺎﺳ ياﺮﺑ نارﺎﻛﻮﺗﺮﭘ هدﻮﺑ ﺖﺳا . ﻦﻳا ﻪﻠﺌـﺴﻣ ﺪـﻳﻮﻣ
ﻦﻳا عﻮﺿﻮﻣ ﺖﺳا ﻪﻛ ﺶﻳﺎﭘ نارﺎﻛﻮﺗﺮﭘ ﺪﻳﺎﺒﻧ ﺎﻬﻨﺗ ﺮﺑ سﺎﺳا يﺮﺘﻤﻳزود يﺎﻫ ﻲﻜﻳﺰﻴﻓ ﻪﻜﻠﺑ مزﻻ ﺖﺳا ﺎـﺗ زا ﺺﺧﺎـﺷ يﺎـﻫ ﻲﻜﻳژﻮـﻟﻮﻴﺑ ﻪـﻛ ردﺎـﻗ ﻪـﺑ ﻪـﺋارا
ﺐﻴﺳآ يﺎﻫ يﻮﺗﺮﭘ يدﺮﻓ هدﻮﺑ ﺰﻴﻧ هدﺎﻔﺘﺳا دﻮﺷ .
هژاوﺪﻴﻠﻛ :ﺎﻫ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ
،p53 ﻮﺗﺮﭘ
،ناﺰﻴﻧﻮﻳ ﺐﻴﺳآ ﻲﻟﻮﻠﺳ
ﺖﻓﺎﻳرد ﺦﻳرﺎﺗ :
10 / 2 / 90 شﺮﻳﺬﭘ ﺦﻳرﺎﺗ
: 19 / 10 / 90
ﻪﺴﻳﺎﻘﻣ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ ردp53 هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ...
37
ﻞﻜﺷ 1 . ﻲﻨﺤﻨﻣ دراﺪﻧﺎﺘﺳا ﻦﻴﺌﺗوﺮﭘ p53
نﺎﻴﺑ p53نژ
ﻻﺎﺑ ﻪﺘﻓر و لﻮﻠـﺳ رد ﻦـﻳا مﺎـﮕﻨﻫ ود هار
ﺶﻴﭘ يور
؛دراد هار لوا ﻦﻳا ﺖﺳا ﻪـﻛ ﺐﻴـﺳآ هدراو ﻪـﺑ
ﺮﻴﻤﻌﺗDNA هﺪﺷ و هار مود ﻦﻳا ﻪـﻛ ﺮـﮔا ﺐﻴـﺳآ هدراو
ﺮﻴﻤﻌﺗ
،ددﺮﮕﻧ لﻮﻠﺳ گﺮﻣ ﻪﻣﺎﻧﺮﺑ يﺰﻳر هﺪـﺷ ) زﻮـﺘﭘﻮﭘآ ( ار
رد ﺶﻴﭘ دﺮﻴﮔ . رد ﻊﻗاو p53 ﺦﺳﺎﭘ
ﻦﻴـﻴﻌﺗ ﻲـﻣ ﺪـﻨﻛ ﻪـﻛ
لﻮﻠﺳ هار ﺗ DNA ﺮﻴﻤﻌ ار
ﺶﻴﭘ دﺮﻴﮔ ﺎﻳ گﺮﻣ يزﻮﺘﻴﻣ ار
لﺎﻌﻓ ﺪﻨﻛ ) 14 و 15 .(
ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ ﺐﻟﺎﻄﻣ ﺮﻛذ هﺪﺷ ناﺰﻴﻣ نﺎﻴﺑ p53نژ
ياﺮﺑ
ﻲﻨﻤﻳا داﺮﻓا يرﺎﻛﻮﺗﺮﭘ ﻪﻛ
ﻪﺑ ظﺎﺤﻟ ﻲﻠﻐﺷ رد ضﺮﻌﻣ ﻮﺗﺮﭘ
راﺮﻗ ﺪﻧراد زا ﺖﻴﻤﻫا ﻲﻳاﺰﺴﺑ ﺮﺑ رادرﻮﺧ ﺖﺳا .
،اﺬﻟ رد ﻦﻳا ﻖﻴﻘﺤﺗ ﻲﻫوﺮﮔ زا نارﺎﻛﻮﺗﺮﭘ ﻪﻛ
رد ضﺮﻌﻣ
ﻢﺋاد ﺎﻳ ﺖﻗﻮﻣ يﺎﻫزود ﺮﻴﻐﺘﻣ زا يﺎﻫﻮﺗﺮﭘ نﻮﻳ
زﺎـﺳ راﺮـﻗ
ﻪﺘﻓﺮﮔ ﺪﻧا ﻪﺑ ناﻮﻨﻋ ﻚﻳ لﺪﻣ ﻲﺘﻴﻌﻤﺟ
رﺎـﻛ ﻪـﺑ و ﻪـﺘﻓﺮﮔ
ناﺰﻴﻣ نﺎﻴﺑ p53نژ
رد ﻦﻳا هوﺮﮔ ﺎـﺑ ﻚـﻳ هوﺮـﮔ ﺪﻫﺎـﺷ
ﺪﻳدﺮﮔ ﻪﺴﻳﺎﻘﻣ .
شور رﺎﻛ
هزاﺪﻧا يﺮـﻴﮔ ناﺰـﻴﻣ نﺎـﻴﺑ ﻦﻴﺌﺗوﺮـﭘ ﻪـﺑp53
ﻚـﻤﻛ
ﺶﻳﺎﻣزآ ياﺰﻳﻻا ﻂـﺳﻮﺗp53
ﺖـﻴﻛ يراﺪـﻳﺮﺧ هﺪـﺷ
زا
ﺖﻛﺮـﺷ Bender MedSystems (BMS256 مﺎـﺠﻧا
ﻪﺘﻓﺮﮔ ﺖﺳا . رﻮﻃ ﻪﺑ ﻲﻠﻛ ياﺮﺑ مﺎـﺠﻧا ﻦـﻳا نﻮـﻣزآ و ﺎـﺑ
ﻪﺟﻮﺗ ﻪﺑ شور رﺎﻛ ﻦـﻳا
،ﺖـﻴﻛ ﻲــﻃ ﻦﻳﺪــﻨﭼ ﻪــﻠﺣﺮﻣ
ﻮﺸﺘﺴﺷ ﺎﺑ يﺎـﻫ لﻮﻠﺤﻣ
،ﻒﻠــﺘﺨـﻣ ﺶﻨـﻛاو
ﻲﻳﺎـﻬﻧ رد
ﺐﻟﺎﻏ ﻚـﻳ لﻮﻠﺤﻣ ﻲﮕﻧر ﻞﺻﺎﺣ ﺖﺳد ﻪﺑ ﻲﻣ
ﺪـﻳآ ﻪــﻛ
تﺪـــﺷ ﮓــﻧر نآ ﺐــﺳﺎﻨﺘﻣ ﺎـــﺑ
راﺪـــﻘﻣ ردp53
ﻪــﻧﻮﻤﻧ
ﺖـﺳا . ياﺮﺑ ﺮﻫ دﺮﻓ ود ﻪﻧﻮﻤﻧ ﻪﻴﻬﺗ و ﺶﻳﺎﻣزآ اﺰـﻳﻻا ﺮـﺑ
يور نآ مﺎﺠﻧا ﻪﺘﻓﺮﮔ و ﻦﻴﮕﻧﺎﻴﻣ ود ﺶﻳﺎـﻣزآ ﻪـﺑ
ناﻮـﻨﻋ
ﺞﻳﺎﺘﻧ ﻪﻌﻟﺎﻄﻣ ﻪﺋارا ﺪﺷ . ﻪﺑ رﻮﻈـﻨﻣ ﻦـﻴﻴـﻌﺗ راﺪــﻘﻣ
،p53
بﺬــــﺟ لﻮــــﻠﺤﻣ
رد 450 ﺮﺘﻣﻮﻧﺎﻧ ﻂــــﺳﻮﺗ هﺎﮕﺘــــﺳد
ناﻮــــﺧاﺰﻳﻻا Anthos
) لﺪــــﻣ 2020
Microplate
Reader (
هزاﺪﻧا ﻪﺘﻓﺮﮔ ﺪﺷ .
ﻲﺳرﺮﺑ يﺎﻫ يرﺎﻣآ ﻪﺴﻳﺎﻘﻣو ناﺰﻴﻣ
نﺎﻴﺑ ﻦﻴﺌﺗوﺮﭘ p53
ﻦﻴــﺑ ود هوﺮــﮔ ﺪﻫﺎــﺷ و رﺎــﻛﻮﺗﺮﭘ ﺎــﺑ
هدﺎﻔﺘــﺳا زا
شور
student t-test مﺎــﺠﻧا
ﺖــﻓﺮﮔ . ﺖــﻬﺟ ﻲــﺳرﺮﺑ ﺮﻴﺛﺎــﺗ
ﺮﻴﻐﺘﻣ يﺎﻫ ﻪﻘﺑﺎﺳ رﺎﻛ
تﺎـﻴﻧﺎﺧد فﺮـﺼﻣ و ﻦـﺳ ﻦﻴـﺑ رد
ﺖـﺴﺗ زا ،رﺎﻛﻮﺗﺮﭘ هوﺮﮔ one way ANOVA
هدﺎﻔﺘـﺳا
ﺪﻣآ ﻞﻤﻋ ﻪﺑ .
نﺎﻴﺑ ناﺰﻴﻣ ﻦﻴﺑ ﻲﮕﺘﺴﺒﻤﻫ ﻦﻴﻴﻌﺗ رﻮﻈﻨﻣ ﻪﺑ
ﻲﮕﺘـﺴﺒﻤﻫ نﻮـﻣزآ ،نارﺎـﻛﻮﺗﺮﭘ يﻮﺗﺮﭘ ﻪﻘﺑﺎﺳ و ﻦﻴﺌﺗوﺮﭘ ﺪﺷ ﻪﺘﻓﺮﮔ رﺎﻛ ﻪﺑ نﻮﺳﺮﻴﭘ .
زا هدﺎﻔﺘﺳا ﺎﺑ يرﺎﻣآ يﺎﻫﺰﻴﻟﺎﻧآ
راﺰﻓا مﺮﻧ Origin 6.1
Excel و ﻢﻴـﺳﺮﺗ و ﻪﺘﻓﺮﮔ مﺎﺠﻧا ﺪﻧﺪﻳدﺮﮔ
. ﻞﻨﺳﺮﭘ درﻮﻣ ﻪـﻌﻟﺎﻄﻣ رد ﻦـﻳا هژوﺮـﭘ زا ﻦﻴـﺑ ﻲﻧﺎـﺒﻠﻃواد
بﺎﺨﺘﻧا هﺪﺷ ﺪﻧا ﻪﻛ رد نﺎﻣزﺎﺳ يژﺮـﻧا ﻲـﻤﺗا ناﺮـﻳا ﻪـﺑ
يﺎﻫ ﺖﻴﻟﺎﻌﻓ نﻮﮔﺎﻧﻮﮔ
يﻮﺗﺮﭘ لﺎﻐﺘﺷا ﺪﻧراد . ﻦﻳا داﺮـﻓا ﺎـﺑ
ﻪﺟﻮﺗ ﻪﺑ ﻞﺤﻣ
،رﺎﻛ تﺪﻣ نﺎﻣز رد ضﺮـﻌﻣ ﻮـﺗﺮﭘ
،ندﻮـﺑ
ﻪﻘﺑﺎﺳ رﺎﻛ و ﺖﻴﻌﺿو ﻲﺘﻣﻼـﺳ بﺎـﺨﺘﻧا
هﺪـﻳدﺮﮔ ﺪـﻧا . رد
عﻮﻤﺠﻣ 42 دﺮﻓ رﺎﻛﻮﺗﺮﭘ رد ﻦﻳا رﺎـﻛ ﻲﺗﺎـﻘﻴﻘﺤﺗ ﺖﻛﺮـﺷ
ﺪﻧدﺮﻛ . ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ هﺪﻧوﺮﭘ ﻲﻜﺷﺰﭘ ﻦﻳا ﻪـﺑ ﺞﻳﺎـﺘﻧ و داﺮـﻓا
،ﺎﻫ نآ ﻲﻜﻳﺰﻴﻓ يﺎﻫﺮﺘﻤﻳزود زا هﺪﻣآ ﺖﺳد زود
ﻲﺘﻓﺎﻳرد
ﺎﻫ نآ زا زود زﺎﺠﻣ ﻪﻧﻻﺎﺳ ياﺮﺑ نارﺎﻛﻮﺗﺮﭘ زوﺎﺠﺗ
هدﺮـﻜﻧ
ﺖﺳا . ﻪﺘﺒﻟا تﺎﻋﻼﻃا طﻮﺑﺮﻣ
ﻪﺑ يﺎﻫزود ﺖﺒﺛ هﺪـﺷ ﻦـﻳا
داﺮﻓا و داﺪﻌﺗ تﺎﻋﺎﺳ رﺎﻛ داﺮﻓا ﺎﺑ ﻮﺗﺮﭘ ﺰﻴﻧ رد سﺮﺘﺳد ﻦﻳا
هوﺮﮔ ﻲﺗﺎﻘﻴﻘﺤﺗ هدﻮﺒﻧ
ﺖﺳا . زا ﻲـﻓﺮﻃ 16 دﺮـﻓ ﺐـﻠﻃواد
ﻪﻛ زا ظﺎﺤﻟ ﻦﺳ و ﻲﺗادﺎﻋ ﺮﻴﻈﻧ لﺎﻤﻌﺘﺳا
،تﺎﻴﻧﺎﺧد ﻞـﻜﻟا
و هﺮﻴﻏ ﺎﺑ هوﺮﮔ رﺎﻛﻮﺗﺮﭘ ﻖﺑﺎﻄﺗ ﻲﻜﻳدﺰﻧ ﺪﻨﺘﺷاد ﻪﺑ ناﻮـﻨﻋ
هوﺮﮔ ﺪﻫﺎﺷ بﺎﺨﺘﻧا ﺪﻧﺪﺷ .
زا ﻦﻳا
،داﺮﻓا ﺎﺑ ﺖﻳﺎﻋر ﻞﺋﺎـﺴﻣ ﻲـﻗﻼﺧا و
نداد ﻲﻫﺎـﮔآ
مزﻻ زا عﻮــﺿﻮﻣ
،ﻖــﻴﻘﺤﺗ 2 ﻲــﺳ ﻲــﺳ نﻮــﺧ
ﻪــﻨﻳرﺎﭙﻫ
ﻪــﺘﻓﺮﮔ ﺪــﺷ . ﺲﭙــﺳ ﺎــﺑ هدﺎﻔﺘــﺳا زا
ژﻮﻔﻳﺮﺘﻧﺎــﺳ )
رد رود
×g 250 و تﺪﻣ ﻪﺑ 10 ﻪﻘﻴﻗد رد يﺎﻣد 20 ﻪﺟرد ﻲﺘﻧﺎـﺳ
داﺮﮔ (
، مﺮـﺳ زا ﻪﻴﻘﺑ ياﺰـﺟا نﻮـﺧ اﺪــﺟ هﺪـﺷ و ياﺮـﺑ
ﺶﻳﺎﻣزآ يﺎﻫ يﺪﻌﺑ رد 80 - ﻪﺟرد داﺮﮔ ﻲﺘﻧﺎﺳ يراﺪﻬﮕﻧ
ﺪﻳدﺮﮔ . رﺎـﻛ ﻪﻘﺑﺎـﺳ و ﺖﻴـﺴﻨﺟ ،ﻦﺳ ﻪﺑ طﻮﺑﺮﻣ تﺎﻋﻼﻃا
لوﺪﺟ رد داﺮﻓا ﻦﻳا يﻮﺗﺮﭘ 1
ﺖﺳا هﺪﻣآ .
ﺎﻫ ﻪﺘﻓﺎﻳ
ياﺮﺑ ﻦﻴﻴﻌﺗ ﺖﻈﻠﻏ ﻦﻴﺌﺗوﺮـﭘ رد
يﺎﻤـﺳﻼﭘ
،ﻦﻴـﺒﻠﻃواد
اﺪﺘﺑا ﺖﻔﻫ ﺖﻈﻠﻏ توﺎﻔﺘﻣ زا ﻚـﻳ ﻪـﻧﻮﻤﻧ ﻦﻴﺌﺗوﺮـﭘ p53
ﺮﺘﻛد ﻼﻬﺷ يﺪﻤﺤﻣ
نارﺎﻜﻤﻫ و 38
لوﺪﺟ 1 . نﺎﺒﻠﻃواد تﺎﺼﺨﺸﻣ
ﻦﻴﺌﺗوﺮﭘ ﺖﻈﻠﻏ U/ml)
(
يﻮﺗﺮﭘ رﺎﻛ ﻪﻘﺑﺎﺳ ﺖﻴﺴﻨﺟ/ﻦﺳ دﺮﻓ هرﺎﻤﺷ (U/ml) ﻦﻴﺌﺗوﺮﭘ ﺖﻈﻠﻏ يﻮﺗﺮﭘ رﺎﻛ ﻪﻘﺑﺎﺳ ﺖﻴﺴﻨﺟ/ﻦﺳ دﺮﻓ هرﺎﻤﺷ 00
/
0 8 دﺮﻣ /31 1R 9/88 2 نز /31 30R
80 /
1 4 دﺮﻣ /25 2R 4/82 7 دﺮﻣ /35 31R
36 /
96 22 دﺮﻣ /46 3R 1/50 1 دﺮﻣ /24 32R
74 /
0 20 دﺮﻣ /52 4R 12/85 9 دﺮﻣ/ 35 33R
29 /
0 10 دﺮﻣ /37 5R 13/72 13 نز /35 34R
25 /
2 23 دﺮﻣ /47 6R 7/07 8 دﺮﻣ /34 35R
82 /
1 20 دﺮﻣ /41 7R 11/44 8 دﺮﻣ /49 36R
21 /
0 23 دﺮﻣ /49 8R 0/13 2 دﺮﻣ /26 37R
16 /
5 17 دﺮﻣ /51 9R 1/96 3 دﺮﻣ /30 38R
75 /
2 20 دﺮﻣ /46 10R 1/58 2 دﺮﻣ /29 39R
01 /
0 10 دﺮﻣ /34 11R 11/10 3 دﺮﻣ /29 40R
80 /
13 12 دﺮﻣ /35 12R 14/54 4 دﺮﻣ /35 41R
27 /
5 12 نز /35 13R 1/64 2 دﺮﻣ /28 42R
13 /
1 24 دﺮﻣ /51 14R 9/27 -- نز/31 1C
42 /
0 10 دﺮﻣ /34 15R 0/29 -- دﺮﻣ/44 2C
54 /
9 7 دﺮﻣ /32 16R 1/27 -- دﺮﻣ/45 3C
07 /
3 11 دﺮﻣ /35 17R 4/50 -- دﺮﻣ/25 4C
58 /
0 2 دﺮﻣ /45 18R 0/31 -- دﺮﻣ/42 5C
26 /
37 8 نز/33 19R 1/21 -- دﺮﻣ/39 6C
27 /
1 18 دﺮﻣ /48 20R 11/76 -- نز/43 7C
01 /
0 3 دﺮﻣ /37 21R 9/22 -- دﺮﻣ/27 8C
77 /
38 20 دﺮﻣ /48 22R 0/05 -- دﺮﻣ/27 9C
14 /
17 20 دﺮﻣ /46 23R 0/44 -- دﺮﻣ/35 10C
78 /
14 8 دﺮﻣ/ 39 24R 5/90 -- دﺮﻣ/30 11C
38 /
6 16 نز /48 25R 3/65 -- دﺮﻣ/28 12C
75 /
42 20 نز /52 26R 4/39 -- دﺮﻣ/51 13C
29 /
45 8 دﺮﻣ /35 27R 0/79 -- دﺮﻣ/29 14C
50 /
4 1 دﺮﻣ /27 28R 4/90 -- دﺮﻣ/36 15C
87 /
1 2 دﺮﻣ /30 29R 1/16 -- دﺮﻣ/36 16C
دراﺪﻧﺎﺘـــــﺳا )
50
، 25
، 5 / 12
، 3 / 6
، 2 / 3
، 6 / 1
، 8 / 0
، 0 / 0
ﻲﺳ ﻲﺳ ﺮﻫ رد ﺪﺣاو (
ﻪﻴﻬﺗ ﺪﻳدﺮﮔ . ﻲـﻨﺤﻨﻣ راﺪﻧﺎﺘـﺳا ﺎـﺑ
ﻢﺳر ﻂـﺳﻮﺘﻣ بﺬـﺟ
ﺖـﻈﻠﻏ دراﺪﻧﺎﺘـﺳا ﺮـﺑ
يور رﻮـﺤﻣ
يدﻮﻤﻋ ﺮﺑ ﺐﺴﺣ p53 ﺖﻈﻠﻏ
ﺮﺑ يور رﻮـﺤﻣ ﻲـﻘﻓا ﻪـﺑ
ﺖﺳد ﺪﻣآ . ﺲﭙﺳ ﻦﻳﺮﺘﻬﺑ ﻂﺧ زا نﺎـﻴﻣ طﺎـﻘﻧ
ﺖـﺳد ﻪـﺑ
،هﺪﻣآ هﺪﻧارﺬﮔ ﺪﺷ ) 99989 / 0 r=
، ﻞﻜﺷ 1 .(
ياﺮﺑ ﻦﻴﻴﻌﺗ ﺖﻈﻠﻏ ﻦﻴﺌﺗوﺮﭘ اﺪﺘﺑاp53
ﻦﻴﮕﻧﺎﻴﻣ بﺬـﺟ
ياﺮﺑ ﺮﻫ ﻪﻧﻮﻤﻧ ﻲﻳﺎﺗود
ﺖـﺳد ﻪـﺑ هﺪـﻣآ
و ﺎـﺑ هدﺎﻔﺘـﺳا زا
ﻲﻨﺤﻨﻣ ﻂـﺧ دراﺪﻧﺎﺘـﺳا )
ﻞﻜـﺷ 1 (
، ﺖـﻈﻠﻏ ﻦﻴﺌﺗوﺮـﭘ رد
ﻪﻧﻮﻤﻧ نﻮﺧ داﺮﻓا ﻦﻴﻤﺨﺗ هدز ﺪﺷ .
رد ﻞﻜﺷ 2 ناﺰﻴﻣ بﺬﺟ ﻪﻧﻮﻤﻧ يﺎﻫ ﻮﺗﺮﭘ رﺎﻛ و ﺪﻫﺎـﺷ
رد 450 ﺮﺘﻣﻮﻧﺎﻧ ﻢﻴﺳﺮﺗ هﺪﺷ ﺖﺳا . راﺪﻘﻣ ﻂﺳﻮﺘﻣ بﺬـﺟ
رد هوﺮﮔ رﺎﻛﻮﺗﺮﭘ 05237 /
±0 27664 / 0 و رد هوﺮﮔ ﺪﻫﺎـﺷ
ﺮــﺑاﺮﺑ ﺎــﺑ 01755 /
±0 14594 / 0 ﺖــﺳد ﻪــﺑ هﺪــﻣآ
ﺖــﺳا
) ﻦﻴﮕﻧﺎﻴﻣ هاﺮﻤﻫ ﻪﺑ ﺖﺳد ﻪﺑ يﺎﻫ SE
ﺖـﺳا هﺪـﺷ ﻪﺋارا (
. ﻻﺎﺑ ندﻮﺑ ناﺰﻴﻣ بﺬﺟ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ياﺮﺑ هوﺮـﮔ رﺎـﻛﻮﺗﺮﭘ
رد ﻪﺴﻳﺎﻘﻣ ﺎﺑ ﺪﻫﺎﺷ ﻼﻣﺎﻛ دﻮﻬﺸﻣ ﺖﺳا .
ﺲﭙــﺳ لوﺪــﺟ ﻊــﻳزﻮﺗ ﻲــﻧاواﺮﻓ ﻦﻴﺌﺗوﺮــﭘ ﻪــﻴﻬﺗp53
هﺪﻳدﺮﮔ و ﺎﺑ هدﺎﻔﺘﺳا زا نآ ﻲﻨﺤﻨﻣ ﻲـﻧاواﺮﻓ ﻦﻴﺌﺗوﺮـﭘ
ﻪـﺑ
ﺖﺳد ﺪﻣآ ) ﻞﻜﺷ 2 .(
ياﺮﺑ ﻪﺴﻳﺎﻘﻣ ود هوﺮﮔ مزﻻ دﻮـﺑ ﺎـﺗ
داﺪﻌﺗ داﺮﻓا رد ﺮﻫ هوﺮﮔ ﻪﺑ 100 هﺰﻳﻻﺎﻣﺮﻧ ﺪﻧﻮﺷ
. ﺎﺑ ﻪﺟﻮـﺗ
ﻪﺑ ﻞﻜـﺷ 2
، ﺖﻈﻠﻏ ﻦﻴﺌﺗوﺮﭘ رد داﺮﻓا رﺎﻛﻮﺗﺮﭘ رد ﻪﺴﻳﺎﻘﻣ
ﺎﺑ داﺮﻓا لﺮﺘﻨﻛ ﺮﺗﻻﺎﺑ هدﻮﺑ و ناﺰﻴﻣ ﻲﮔﺪﻨﻛاﺮﭘ نآ
رد هوﺮﮔ
رﺎﻛﻮﺗﺮﭘ ﺮﺘﺸﻴﺑ ﺖﺳا ) 0225 / 0 p= (.
ﻪـﭽﻧﺎﻨﭼ ﻞﻜـﺷ رد
2
هﺪﻳد ﻲﻣ
،دﻮﺷ رد هوﺮﮔ رﺎﻛﻮﺗﺮﭘ يداﺮﻓا ﺎﺑ ناﺰﻴﻣ ﺮﺗﻻﺎﺑ زا
30
ﻲـﺳ ﻲـﺳ ﺮﻫ رد ﺪﺧاو ﻦﻴﺌﺗوﺮـﭘ زا
p53 ﺰـﻴﻧ هﺪـﻳد
ﻲﻣ ﺪﻧﻮﺷ . رﺎـﻛ ﻪﻘﺑﺎـﺳ ﺐـﺴﺣ ﺮـﺑ ﻦﻴﺌﺗوﺮـﭘ ﻊـﻳزﻮﺗ ﻦﻳا ﺮﺑ هوﻼﻋ رد ﻪـﻛ ﺖـﻓﺮﮔ راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﺰﻴﻧ رﺎﻛﻮﺗﺮﭘ داﺮﻓا يﻮﺗﺮﭘ
ﻞﻜﺷ 3
ﺖـﺳا هﺪـﺷ هداد نﺎﺸﻧ .
ﺐﻳﺮـﺿ زا هدﺎﻔﺘـﺳا ﺎـﺑ
ﻪﺴﻳﺎﻘﻣ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ ردp53 هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ...
39
ﺪﻫﺎﺷ و رﺎﻛﻮﺗﺮﭘ هوﺮﮔ ود ردP53 ﻦﻴﺌﺗوﺮﭘ ﺮﻳدﺎﻘﻣ ماﺮﮔﻮﺘﺴﻴﻫ .2ﻞﻜﺷ
ﻞﻜﺷ 3 . داﺮﻓا يﻮﺗﺮﭘ رﺎﻛ ﻖﺑﺎﺳ ﺐﺴﺣ ﺮﺑ ﻦﻴﺌﺗوﺮﭘ ﺖﻈﻠﻏ
راﺮـﻗ ﻲﺳرﺮﺑ درﻮﻣ ﺎﻫ نآ ﻦﻴﺑ طﺎﺒﺗرا نﻮﺳﺮﻴﭘ ﻲﮕﺘﺴﺒﻤﻫ يرﺎـﻣآ ﺰﻴﻟﺎـﻧآ ﻦـﻳا ﺞﻳﺎﺘﻧ ﻪﻛ ﺖﻓﺮﮔ )
27 / 0 r= ( زا ﻲﻛﺎـﺣ
راد ﻲﻨﻌﻣ ﻲﮕﺘﺴﺒﻤﻫ مﺪﻋ ﻘﺑﺎﺳ و ﻦﻴﺌﺗوﺮﭘ نﺎﻴﺑ ﻦﻴﺑ
ﻪ رﺎـﻛ
ﺖﺳا نﺎﺒﻠﻃواد يﻮﺗﺮﭘ .
نﺎـﻴﺑ درﻮـﻣ رد ﻲﻬﺑﺎـﺸﻣ ﻲﺳرﺮﺑ
ﺰﻴﻧ ﻲﺳرﺮﺑ ﻦﻳا ﻪﻛ ﺖﻓﺮﮔ مﺎﺠﻧا نﺎﺒﻠﻃواد ﻦﺳ و ﻦﻴﺌﺗوﺮﭘ نﺎﺸﻧ ار ﻦﻴﺌﺗوﺮﭘ نﺎﻴﺑ ناﺰﻴﻣ و ﺐﻠﻃواد ﻦﺳ ﻦﻴﺑ ﻲﻃﺎﺒﺗرا داﺪﻧ ) ﺖﺳا هﺪﺸﻧ هداد نﺎﺸﻧ ﺞﻳﺎﺘﻧ .(
ﻪﻌﺟاﺮﻣ ﺎﺑ ﺎﻬﻨﺗ ،هﺪﺷ ﺮﭘ يﺎﻫ مﺮﻓ ﻪﺑ
7 % و نارﺎﻛﻮﺗﺮﭘ زا
5 / 6 % ﺪـﻧدﻮﺑ يرﺎﮕﻴـﺳ ﺪﻫﺎـﺷ داﺮﻓا زا )
ﺎـﻫ هداد ﺶﻳﺎـﻤﻧ
ﻫداد هﺪﺸﻨ ﺪﻧا ( . طﺎﺒﺗرا ﻲﻨﻌﻣ و يرﺎﮕﻴـﺳ داﺮﻓا ﻦﻴﺑ ﺎﻣ يراد
ﻦﻴﺌﺗوﺮـﭘ نﺎـﻴﺑ ظﺎﺤﻟ ﻪﺑ يرﺎﮕﻴﺳ ﺮﻴﻏ ﺪـﺸﻧ هﺪـﻳدP53
) 1265 / 0 p= (.
ﺚﺤﺑ و ﻪﺠﻴﺘﻧ يﺮﻴﮔ
تﺎﻌﻳﺎﺿ ﻲـﺷﺎﻧ زا يﺎـﻫﻮﺗﺮﭘ ناﺰﻴﻧﻮـﻳ
DNA رد لﻮﻠـﺳ
ﺚﻋﺎﺑ زوﺮﺑ هﺮﺘﺴﮔ يا زا ﺦـﺳﺎﭘ ﺎـﻫ ﻞـﺜﻣ ﻒـﻗﻮﺗ ﻞﻜﻴـﺳ
،ﻲﻟﻮﻠﺳ
،زﻮﺘﭘﻮﭘآ گﺮﻣ يزﻮﺘﻴﻣ و ﻲﮔﺪﺷﺮﻴﭘ ﻲﻣ
دﻮﺷ . ﻚﻳ
يﺮﮔ ﻮﺗﺮﭘ ﻲﻣ ﺪﻧاﻮﺗ دوﺪﺣ 40 ﺖـﺴﻜﺷ ود
ﻪﺘـﺷر يا رد
دﻮﺟﻮﺑDNA هدروآ و ﻂﻘﻓ ﻚﻳ ﺖـﺴﻜﺷ ود
ﻪﺘـﺷر يا
ﻢﻴﻣﺮــﺗ
،هﺪــﺸﻧ ﻲﻓﺎــﻛ ﺖــﺳا ﺎــﺗ گﺮــﻣ ﻲﻟﻮﻠــﺳ ار ﻢــﻗر
ﺪــﻧز ) 16 و 17 .(
رد ﻊــﻗاو ﻲــﻤﻛ ﺲــﭘ زا ﺶﺑﺎــﺗ يﺎــﻫﻮﺗﺮﭘ
،ناﺰﻴﻧﻮﻳ ﻦﻴﺌﺗوﺮﭘ ﻪﺑp53
ناﻮﻨﻋ ﻚﻳ ) ﻆﻓﺎﺤﻣ مﻮـﻧژ ( ﻪـﺑ
يﺎﻫ ﻲﻟاﻮﺗ ﻲﺻﺎﺧ
DNA زا ) ﻲﺣاﻮـﻧ رد ﻢﻴـﻈﻨﺗ
هﺪـﻨﻨﻛ
نژ ﺎﻫ ( ﻞﺼﺘﻣ ﻲﻣ دﻮﺷ . ﻦـﻳا طﺎـﻘﻧ ﻞﻣﺎـﺷ
يﺎـﻫ نﺎـﻜﻣ
لﺮﺘﻨﻛ هﺪﻨﻨﻛ ﻞﻜﻴﺳ
،ﻲﻟﻮﻠﺳ ﻢﻴﻈﻨﺗ هﺪﻨﻨﻛ زﻮـﺘﭘﻮﭘآ و ﺰـﻴﻧ
نﺎﻜﻣ ﻲﻳﺎﻫ ﺖﺳا ﻪﻛ ﻪﺑ رﻮﻃ ﻲﻔﻨﻣ p53 دﻮـﺧ
ار ﻢﻴـﻈﻨﺗ
ﻲﻣ ﺪﻨﻨﻛ . ﺎﭘ p53 ﺦـﺳ ﻦﻴـﻴﻌﺗ هﺪـﻨﻨﻛ ﺖـﺷﻮﻧﺮﺳ لﻮﻠـﺳ
ﺐﻴﺳآ هﺪﻳد ﺖﺳا ﻪﻛ ﺎﻳ ﻢﻴﻣﺮﺗ وDNA ﺎﻳ گﺮﻣ يزﻮﺘﻴﻣ
ار ﻲﻃ .ﺪﻨﻛ ﻦﻳاﺮﺑﺎﻨﺑ ﻦﻴﺌﺗوﺮﭘ ﻚﻳp53
نژ زﺎﺑ هﺪـﻧراد ي
يرﻮﻣﻮﺗ ﺖﺳا و ﺎﺗ ﻲﻧﺎﻣز ﻪﻛ لﻮﻠﺳ ﻲﻣ ﺪـﻧاﻮﺗ ﺪـﻴﻟﻮﺗp53
،ﺪﻨﻛ ﻊﻧﺎﻣ ﻪﻌﺳﻮﺗ ي نﺎﻃﺮﺳ ﻲﻣ ددﺮﮔ .
راﺪﻘﻣ ردp53 لﻮﻠﺳ يﺎﻫ ﻲﻌﻴﺒﻃ ﻢﻛ ﺖﺳا و ﻪﺑ رﻮـﻃ
ﺮﺘﻛد ﻼﻬﺷ يﺪﻤﺤﻣ
نارﺎﻜﻤﻫ و 40
ﻪﺘﺳﻮﻴﭘ ﺪـﻴﻟﻮﺗ و ﺐـﻳﺮﺨﺗ ﻲـﻣ
دﻮـﺷ . رد ترﻮـﺻ ﺐﻴـﺳآ
،DNA ﺐﻳﺮﺨﺗ ﻦﻴﺌﺗوﺮﭘ ﻒﻗﻮﺘﻣp53
هﺪﺷ و راﺪﻘﻣ p53
رد لﻮﻠﺳ ﻪﺑ تﺪﺷ ﺶﻳاﺰﻓا اﺪﻴﭘ ﻲﻣ ﺪـﻨﻛ . ﻦﻴﺌﺗوﺮـﭘ p53
ﻪﺑ نژ ﻲﻳﺎﻫ ﻪﻛ زﺎﺑ يزﺎـﺳ ارDNA
ﻢﻴـﻈﻨﺗ ﻲـﻣ
،ﺪـﻨﻨﻛ
ﻞﺼﺘﻣ هﺪـﺷ و گﺮـﻣ ﻪـﻣﺎﻧﺮﺑ يﺰـﻳر هﺪـﺷ ي لﻮﻠـﺳ ار
لﺮﺘﻨﻛ ﻲﻣ ﺪﻨﻛ . ﺢﻄﺳ دﺎـﻳز ﻦﻴﺌﺗوﺮـﭘ رد p53
ﻪـﻠﺣﺮﻣ ي
،لوا ﻪﺧﺮﭼ ي ﻲﻟﻮﻠﺳ ار ﻒﻗﻮﺘﻣ ﻲﻣ ﺪﻨﻛ ﺎﺗ ﺖﺻﺮﻓ ياﺮـﺑ
يزﺎﺳزﺎﺑ ﺐﻳﺮﺨﺗDNA
هﺪﺷ ﻪﺑ دﻮﺟو ﺪﻳآ . ﺮﮔا ﺐـﻳﺮﺨﺗ
رﺎﻴﺴﺑ ﺪﻳﺪـﺷ
،ﺪـﺷﺎﺑ لﻮﻠـﺳ گﺮـﻣ ﻪـﻣﺎﻧﺮﺑ يﺰـﻳر هﺪـﺷ
و
» ﻲﺸﻛدﻮﺧ لﻮﻠﺳ
« ار زﺎـﻏآ ﻲـﻣ ﺪـﻨﻛ . ﻲـﻟو زا ﻲـﻓﺮﻃ
تﺎﻌﻟﺎﻄﻣ نﺎﺸﻧ
هداد ﺪﻧا ﻪﻛ رد ﻞﻗاﺪﺣ 50 ﺪﺻرد زا مﺎـﻤﺗ
نﺎﻃﺮﺳ p53،ﺎﻫ
دﻮﺧ رﺎﭼد ﺮﻴﻴﻐﺗ هﺪﻳدﺮﮔ ﺖـﺳا ) 11 - 9 ( .
تﺎﻘﻴﻘﺤﺗ ﻪﻠـﺻﺎﺣ
ﺮـﺑ يور ﻦـﻳا نژ ﻲـﺸﺣو وp53
ﺎـﻳ
ﺖﻟﺎﺣ ﺶﻬﺟ ﻪـﺘﻓﺎﻳ ﻦـﻳا
،نژ ﻲﻛﺎـﺣ زا ﻦـﻳا ﺖـﺳا ﻪـﻛ
ﺖﻴﺳﺎﺴﺣ يﻮﺗﺮﭘ
ﻪﺑ رﻮﻃ ﻂﺳﻮﺘﻣ ﺎﺑ دﻮﺒﻤﻛ ﻦﻴﺌﺗوﺮﭘ p53
ﺶﻫﺎﻛ ﻲﻣ ﺪﺑﺎﻳ ) 18 .(
رد ﺶﻫوﮋﭘ ﺮﺿﺎﺣ ﺮﺑ يور داﺮﻓا ﻂﺒﺗﺮﻣ ﺎﺑ
،ﻮﺗﺮﭘ ناﺰـﻴﻣ
ردp53 مﺮﺳ نﻮﺧ ﻲﻄﻴﺤﻣ ﺎﺑ ﻚﻳ هوﺮﮔ ﺪﻫﺎـﺷ ﻪـﺴﻳﺎﻘﻣ
هﺪﺷ ﺖﺳا . ﺞﻳﺎﺘﻧ ﻦﻳا هزاﺪﻧا يﺮﻴﮔ ﻲﻛﺎﺣ زا ﺮﺗﻻﺎـﺑ ندﻮـﺑ
ﻦﻳا ﻦﻴﺌﺗوﺮﭘ رد يﺎﻤﺳﻼﭘ هوﺮـﮔ
رﺎـﻛﻮﺗﺮﭘ ﺖـﺳا
. ﻦـﻜﻤﻣ
ﺖﺳا نﺎﻴﺑ يﻻﺎﺑ ﻦﻴﺌﺗوﺮﭘ سﺎﻜﻌﻧا رﻮﻀﺣ مواﺪﻣ ﻮـﺗﺮﭘ رد
ﻂﻴﺤﻣ رﺎﻛ و سﺮﺘﺳا ﻲﺷﺎﻧ زا نآ ﻪﺑ لﻮﻠﺳ ﺪﺷﺎﺑ ﻪـﻛ ﻪـﺑ
ﺶﻳاﺰﻓا ﻪﺑ p53
رﻮﻈﻨﻣ ترﺎﻈﻧ ﻪﺑ ﺐﻴـﺳآ DNAيﺎـﻫ
ﺮﺠﻨﻣ هﺪﺷ ﺖﺳا . رد ﻊﻗاو ﻦﻳا ﺢﻄﺳ يﻻﺎﺑ نﺎﻴﺑ نژ يﺎﻫ
يﺮﻴﻤﻌﺗ ردDNA
رﺎﻛﻮﺗﺮﭘ ﻲﻣ ﺪﻧاﻮﺗ ﻲﺷﺎﻧ زا لﺎﻌﻓ نﺪﺷ
ﺮﻴﺴﻣ يﺎﻫ ﺮـﻴﻤﻌﺗ وDNA
رد ﺦـﺳﺎﭘ ﻪـﺑ تاﺮـﺛا هﺎـﺗﻮﻛ
تﺪﻣ و ﺰﻴﻧ ﺪﻨﻠﺑ تﺪﻣ يﺎﻫزود ﻦﻴﻳﺎﭘ ﻮﺗﺮﭘ ﺪـﺷﺎﺑ
؛ ﺪـﻨﭼﺮﻫ
ﻪﻛ ﻴﻣ ناﺰ نﺎﻴﺑ ﻦﻳا نژ رد ﻲﺧﺮﺑ زا داﺮﻓا رﺎﻛﻮﺗﺮﭘ ) داﺮـﻓا
هرﺎﻤﺷ 3
، 19
، 22
، 26 و 27 ( رﺎﻴﺴﺑ ﻻﺎﺑ هدﻮـﺑ و مﺰﻠﺘـﺴﻣ
ﻲﺳرﺮﺑ يﺎﻫ ﺮﺘﺸﻴﺑ ﻲﻣ ﺪﺷﺎﺑ .
رد ﻲــﺷراﺰﮔ Rössnerﻪــﻛ
و ﺶﻧارﺎــﻜﻤﻫ )
19 ( ﻪــﺋارا
هدﺮﻛ
،ﺪﻧا ناﺰﻴﻣ نﺎﻴﺑ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ار رد يﺎﻤـﺳﻼﭘ هوﺮـﮔ
رﺎﻛﻮﺗﺮﭘ ﻞﺑﺎﻗ ﻪﺴﻳﺎﻘﻣ ﺎﺑ هوﺮﮔ ﺪﻫﺎﺷ ﻪـﺘﻓﺎﻳ ﺪـﻧدﻮﺑ ﻪـﻛ ﺎـﺑ
ﺞﻳﺎﺘﻧ ﻪﺋارا هﺪﺷ رد ﻦﻳا ﻖﻴﻘﺤﺗ ﺖﻘﺑﺎﻄﻣ
؛دراﺪﻧ ﺪﻨﭼﺮﻫ ﻪﻛ
نآ ﺎﻫ ناﺰﻴﻣ نآ ار رد ﺖﻴﺳﻮﻔﻨﻟ نارﺎﻛﻮﺗﺮﭘ
ﺮﺘﺸﻴﺑ شراﺰـﮔ
ﺪﻧدﺮﻛ . تﺎﻘﻴﻘﺤﺗ يدﺪﻌﺘﻣ
نﺎﺸﻧ ﻲﻣ ﺪـﻨﻫد ﻪـﻛ ﻦﻴﺌﺗوﺮـﭘ
،ﻲﻌﻴﺒﻃp53 نوﺪﺑ ﻲﺸﻬﺟ DNAرد
ﻦـﻜﻤﻣ ﺖـﺳا رد
ﺎﻫرﻮﻣﻮﺗ ﻪﺘﺷﺎﺒﻧا ﺪﻧﻮﺷ .
هوﻼﻋ ﺮﺑ
،ﻦﻳا ﺎﺑ ﺶﻧاد
،ﺎﻣ رد هداﻮﻧﺎﺧ يﺎﻫ ﺪﻌﺘـﺴﻣ ﻪـﺑ
،نﺎﻃﺮﺳ ﻦﻴﺌﺗوﺮﭘ ﻦﻜﻤﻣp53
ﺖﺳا رد ﺖﻓﺎﺑ يﺎـﻫ ﺮـﻴﻏ ﻚﻴﺘﺳﻼﭘﻮﺌﻧ
ﺎﻳ رد ﺖﻓﺎﺑ يﺎﻫ ﺎﺑ لﻮﻠﺳ يﺎﻫ ﻲـﻌﻴﺒﻃ ﺰـﻴﻧ
ﻊﻤﺠﺗ ﺪﻨﺑﺎﻳ ) 20 و 21 .(
ﺮﻫ ود ﻞﻣاﻮﻋ ﻲﻜﻴﺘﻧژ و ﻴﻄﻴﺤﻣ ﻲﻤ ﺪﻨﻧاﻮﺗ دﺮـﻜﻠﻤﻋ p53
ار ﻞﻳﺪــﻌﺗ ﺪــﻨﻳﺎﻤﻧ . ﺢــﺿاو ﺖــﺳا ﻪــﻛ تاﺮــﻴﻴﻐﺗ رد
نﺎــﻴﺑ
ﻦﻴﺌﺗوﺮﭘ ﺮﺠﻨﻣp53
ﻪﺑ تﻻﻼﺘﺧا رد
ﻢﻴﻈﻨﺗ ﻪﺧﺮﭼ ﻲﻟﻮﻠﺳ
ﻲﻣ ددﺮﮔ ) 22 .(
ﻦﻴﺌﺗوﺮﭘ لﺎﻣﺮﻧp53
ﻲﻣ ﺪﻧاﻮﺗ لﻮﻠﺳ ﺎﻫ ار
رد يﺎﻬﺘﻧا G1 زﺎﻓ
ﻪﺧﺮﭼ ﻲﻟﻮﻠـﺳ ﻲـﻨﻌﻳ ﺖـﺳرد ﻞـﺒﻗ زا
DNAﺰﺘﻨﺳ ﻒﻗﻮﺘﻣ
،دزﺎﺳ رد ﻲﻟﺎﺣ ﻪـﻛ لﺎﻜـﺷا ﺶـﻬﺟ
ﻪﺘﻓﺎﻳ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ﻖﻓﻮﻣ ﻪﺑ ﻒﻗﻮﺗ ﻪﺧﺮﭼ ﻲﻟﻮﻠﺳ هﺪـﺸﻧ و
ﺮﻴﺜﻜﺗ ﻲﻟﻮﻠﺳ ﻪﻣادا ﻲﻣ ﺪﺑﺎﻳ . يرﺎﻴﺴﺑ زا تﺎﻌﻟﺎﻄﻣ رد
درﻮﻣ
p53نﺎﻴﺑ ﺮﺑ ﺖﻴﻤﻫا ﻪـﻤﻴﻧ ﺮـﻤﻋ ﻲﻧﻻﻮـﻃ ﺶـﻬﺟ p53
ﻪﺘﻓﺎﻳ ﺪﻴﻛﺎﺗ ﻲﻣ ﺪﻨﻨﻛ ﻪـﻛ ﺮـﺠﻨﻣ ﻪـﺑ ﻊـﻤﺠﺗ ﻦﻴﺌﺗوﺮـﭘ رد
ﺖﻓﺎﺑ يﺎﻫ ﻚﻴﺘﺳﻼﭘﻮﺌﻧ )
23 ( ﻲﻣ دﻮﺷ . ﻲﻟو نﺎـﻴﺑ رﺎﻴـﺴﺑ
يﻻﺎـﺑ ﻦﻴﺌﺗوﺮـﭘ ﻲــﺸﺣوp53
رد ﻪـﻧﻮﻤﻧ يﺎـﻫ يرﻮــﻣﻮﺗ
،نﺎﺴﻧا و ﺰﻴﻧ رد تﺎﻌﻳﺎﺿ شﻮﺧ ﻢﻴﺧ نوﺪﺑ p53ﺶـﻬﺟ
ﺰﻴﻧ هﺪﻫﺎﺸﻣ هﺪﺷ ﺖﺳا ) 20 .(
ﻦﻳاﺮﺑﺎﻨﺑ نﺎﻴﺑ يﻻﺎـﺑ ﻦﻴﺌﺗوﺮـﭘ
،p53 ﻦـﻜﻤﻣ ﺖـﺳا
ًﺎﻣوﺰﻟ ﻪﺑ ﺖﻠﻋ ﺶﻬﺟ رد ﻲﺣاﻮـﻧ ﺪـﻛ هﺪـﻨﻨﻛ نوﺰـﮔا نژ
ﺪﺷﺎﺒﻧ ) 24 .(
زا ﻪﻛ ﻲﻳﺎﺠﻧآ ﺶﻬﺟ
رد p53نژ ﻚﻳ ﻞـﻣﺎﻋ
ﺶﻴﭘ ﻲﻬﮔآ هﺪﻨﻫد ﻢﻬﻣ هدﻮﺑ ﻪـﻛ
ﺪـﻧاﻮﺗ ﻲـﻣ ﺮـﺠﻨﻣ
ﻪـﺑ
ﻊﻤﺠﺗ ﻦﻳا ﻦﻴﺌﺗوﺮﭘ رد ﻪﺘﺴﻫ يﺎﻫ لﻮﻠﺳ يﺎﻫ ﻲﻧﺎﻃﺮـﺳ
ددﺮﮔ ) 27 - 25 (
، ﻲـﺳرﺮﺑ ﻦـﻳاPCR
نژ و هزاﺪـﻧا يﺮـﻴﮔ
،زﻮﺘﭘﻮﭘآ تﺎﻋﻼﻃا ﻊﻣﺎﺟ
يﺮﺗ رد درﻮﻣ ﻢﺴﻴﻧﺎﻜﻣ دﺮـﻜﻠﻤﻋ
ﻦﻳا ﻦﻴﺌﺗوﺮﭘ رد درﻮﻣ ﻦﻳا هوﺮﮔ يرﺎـﻛ رد رﺎـﻴﺘﺧا ﺪـﻫاﻮﺧ
ﺖﺷاﺬﮔ ) 28 .(
ﺞﺑﺎﺘﻧ ﻞﺻﺎﺣ زا ﺰﻴﻟﺎﻧآ يرﺎﻣآ نﺎﺸﻧ هداد ﻪﻛ ناﺰﻴﻣ نﺎـﻴﺑ
ﻦﻳا ﻦﻴﺌﺗوﺮﭘ ﻪﺑ ﻪﻘﺑﺎﺳ رﺎﻛ داﺮﻓا و ﺰﻴﻧ ﻪﺑ فﺮﺼﻣ تﺎﻴﻧﺎﺧد
ﻲﮕﺘــﺴﺑ دراﺪــﻧ
p>0.05 ) .(
ﻦــﻳا ﺞﻳﺎــﺘﻧ رد ﻖﺑﺎــﻄﺗ ﺎــﺑ
تاﺪﻫﺎﺸﻣ Rössner
و ﺶﻧارﺎـﻜﻤﻫ )
29 ( و Garaj-ﺰـﻴﻧ
Vrhovac و
شرﺎﻜﻤﻫ )
30 ( ﻲﻣ ﺪﺷﺎﺑ .
هزوﺮﻣا دروآﺮـﺑ تاﺮـﻄﺧ ﻲـﺷﺎﻧ
زا زود يﺎـﻫ ﻦﻴﻳﺎـﭘ رد
،ﻂﻴﺤﻣ ﻲﺸﻟﺎﭼ ﻲﻣﻮﻤﻋ بﻮﺴﺤﻣ ﻲـﻣ
دﻮـﺷ . رد ود ﻪـﻫد
ﺮﻴﺧا ﻢﻏر ﻲﻠﻋ ﺶﻳاﺰﻓا
هدﺎﻔﺘﺳا زا ﻮﺗﺮﭘ رد
،ﻲﻜﺷﺰﭘ ﻮـﺗﺮﭘ
يﺮﻴﮔ يﺎﻫ ﻲﻠﻐﺷ ﺶﻫﺎﻛ ﻪﺘﻓﺎﻳ ﺖﺳا . ﻲﻜﻳ زا ﻞﻳﻻد ﻦﻳا
ﺮﻣا يوﺮـﻴﭘ ﺮﺘـﺸﻴﺑ نارﺎـﻛﻮﺗﺮﭘ زا
تارﺮـﻘﻣ و
شور يﺎـﻫ
ﻲﻨﻤﻳا نوﺪﻣ ﺖﺳا ﻪﺑ يﻮﺤﻧ ﻪﻛ يﺮﻴﮔﻮـﺗﺮﭘ نﺎـﻨﻛرﺎﻛ
رد
هدوﺪﺤﻣ زود زﺎﺠﻣ راﺮﻗ ﻪﺘﻓﺮﮔ
؛ﺖﺳا هدوﺪﺤﻣ يا ﻪﻛ ﻪـﺑ
نآ هدوﺪﺤﻣ ﻲﺑ ﺮﻄﺧ قﻼﻃا ﻲﻣ ددﺮـﮔ . ﻲـﻟو رد ﻖـﻴﻘﺤﺗ
ﺮﺿﺎﺣ ناﺰﻴﻣ نﺎﻴﺑ ﻦﻴﺌﺗوﺮﭘ ردp53
ﻦﻴﺑ نارﺎﻛﻮﺗﺮﭘ ﺮﺗﻻﺎﺑ
زا داﺮﻓا ﺪﻫﺎﺷ هﺪﻫﺎﺸﻣ هﺪﺷ ﺖـﺳا . رد ﻲﻟﺎـﺣ ﻪـﻛ ﺞﻳﺎـﺘﻧ
ﻪﺴﻳﺎﻘﻣ زوﺮﺑ ﻦﻴﺌﺗوﺮﭘ ردp53 هوﺮﮔ نارﺎﻛﻮﺗﺮﭘ ...
41
ﺶﻳﺎﭘ ﻦﻳا نارﺎﻛﻮﺗﺮﭘ ﻲﻛﺎﺣ
زا ﺖﻓﺎﻳرد يزود رد هدوﺪﺤﻣ
زود زﺎﺠﻣ ﻪﻧﻻﺎﺳ ياﺮﺑ نارﺎﻛﻮﺗﺮﭘ هدﻮﺑ
ﺖﺳا .
ﻦﻳا ﺞﻳﺎﺘﻧ نﺎﺸﻧ ﻲﻣ ﺪﻫد ﻪﻛ يﺮﺘﻤﻳزود يﺎـﻫ
ﻲـﻜﻳﺰﻴﻓ
ﺪﻳﺎــﺷ تﺎــﻋﻼﻃا ﻲﻠﻣﺎــﻛ
ار رد درﻮــﻣ ﻊــﻳزﻮﺗ و ناﺰــﻴﻣ
يﺮﻴﮔﻮﺗﺮﭘ داﺮﻓا
نﺎﺸﻧ ﻲﻤﻧ ﺪﻫد . رد ﻪﻛ ﻲﻟﺎﺣ ﺎﺑ
ﻪـﺟﻮﺗ ﻪـﺑ
تﺎــﻴﺑﺮﺠﺗ ﻞــﺻﺎﺣ
زا ثداﻮــﺣ
،يﻮــﺗﺮﭘ ﺺﺧﺎــﺷ
يﺎــﻫ
ﻲﻜﻳژﻮﻟﻮﻴﺑ ﻪﺑ
رﻮﻈﻨﻣ ﺖﺳد ﻪﺑ ندروآ
تﺎﻋﻼﻃا طﻮﺑﺮﻣ
ﻪﺑ
ﻊﻳزﻮﺗ ناﺰﻴﻣو يﺮﻴﮔﻮﺗﺮﭘ رﺎﻴﺴﺑ
ﻲﺗﺎﻴﺣ ﺖﺳا .
ﻪﺑ هوﻼﻋ زا ﻪﻛ ﻲﻳﺎﺠﻧآ داﺮﻓا
ياراد ﺖﻴﺳﺎﺴﺣ يﻮـﺗﺮﭘ
ﻲﺗوﺎﻔﺘﻣ
،ﺪﻨﺘﺴﻫ ﺺﺧﺎﺷ يﺎﻫ ﻲﻜﻳژﻮـﻟﻮﻴﺑ ياراد
ﺖـﻳﺰﻣ
يﺮﺘﺸﻴﺑ ﺪﻨﺘﺴﻫ .
،ﻦﻳاﺮﺑﺎﻨﺑ ياﺮﺑ نﺎﻨﻴﻤﻃا زا دﻮﺟو ﺮﺜﻛاﺪﺣ
ﻲﻨﻤﻳا
،ﻲﻠﻐﺷ ﺶﻳﺎﭘ ﻲﻜﻳژﻮـﻟﻮﻴﺑ نارﺎـﻛﻮﺗﺮﭘ
ياراد شزرا
يرﺎﻴﺴﺑ ﺖﺳا . ﻦﻴﻨﭼ شور ﻲﻳﺎـﻫ ﻲـﻣ ﺪـﻧاﻮﺗ ﻪـﺑ ناﻮـﻨﻋ
ﺮﺘﻤﻳزود ﻲﻠﺧاد ياﺮـﺑ ﺶﻳﺎـﭘ دﺮـﻓ و عﻼـﻃا زا ﺶﻳاﺰـﻓا
ﻲﻟﺎﻤﺘﺣا ﻞﻣاﻮﻋ ﻚﻴﺴﻛﻮﻧژ و
ًﻻﺎﻤﺘﺣا ﺮﻄﺧ نﺎﻃﺮﺳ ﻲـﻳاز
رد ﺮﻈﻧ ﻪﺘﻓﺮﮔ دﻮﺷ .
ﺮﻜﺸﺗ و ﺮﻳﺪﻘﺗ
ﻦﻳﺪﺑ ﻪﻠﻴﺳو زا قوﺪﻨـﺻ ﺖـﻳﺎﻤﺣ
زا ناﺮﮕـﺸﻫوﮋﭘ ﻪـﻛ
ﻲﺸﺨﺑ زا ﻪﺟدﻮﺑ ﻦﻳا رﺎﻛ ﻲﺗﺎﻘﻴﻘﺤﺗ ار
ﻦﻴﻣﺎﺗ هدﻮﻤﻧ ﺖـﺳا
ﺮﻜﺸﺗ ﻲﻣ دﻮﺷ . ﻦﻴﻨﭽﻤﻫ زا
يﺎﻗآ ﺮﺘﻛد ﻲﻫﺎﺷﺮﻴﻣ و
ﺪـﺣاو
ﺖﻇﺎﻔﺣ رد ﺮﺑاﺮﺑ ﻪﻌﺷا ﻪـﻛ هﺎﮕـﺸﻳﺎﻣزآ يﺎـﻫ
دﻮـﺧ ار رد
رﺎﻴﺘﺧا ﻦﻳا هوﺮﮔ ﻲـﺸﻫوﮋﭘ راﺮـﻗ
هداد
،ﺪـﻧا يراﺰﮕـﺳﺎﭙﺳ
ﻲﻣ ددﺮﮔ .
ﻊﺑﺎﻨﻣ
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نارﺎﻜﻤﻫ و 42
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Original Article
Comparison of p53 expression between occupationally exposed to ionizing radiation and control group
*Shahla Mohammadi, Ph.D. Assistant Professor of Molecular Biophysics, Nuclear Science Research School, Nuclear Science & Technology Research Institute, Tehran, Iran (*Corresponding author). [email protected]
Mohammad Reza Gharaati, MSc. PhD candidate of Biochemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran. [email protected]
Kobra Ezaki Khan Alang, MSc. PhD candidate of Molecular Medicine, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. [email protected]
Rasa Rajaie, MSc. Radiation Applications Research School, Nuclear Science & Technology Research Institute, Tehran, Iran. [email protected]
Abstract
Background: Various stresses such as ionizing radiation can increase cellular damage, especially to nuclear DNA. To protect cellular damages, normal regulatory genes (such as Tp53 tumor suppressor) become activated. Accordingly, in this study, the p53 gene and its expression among employees occupationally exposed to low doses of ionizing radiation were compared with a selected control group.
Methods: Using an ELISA kit, the amount of p53 protein in the blood serum of case and control groups was measured. Statistical analysis was performed using student t-test procedure. As a case group, 42 healthy individuals (with a mean age of 37.8 ± 1.3 years) occupationally dealing with different kind of radiation sources in Atomic Energy Organization of Iran were chosen. As a control group, 16 healthy unexposed volunteers (with a mean age of 34.5 ± 2.0 years) who were matched for age, sex and smoking habits were selected. A 2 ml aliquot of heparinized peripheral blood was collected from each case.
Results: The results of this research indicate the higher levels of p53 expression in occupationally radiation group. Moreover, the work history or smoking had no effects on p53 expression.
Conclusion: Although the absorbed doses were below the permissible limits, this study confirms the role of low-level chronic exposure in increasing p53 expression among occupationally radiation workers. These results confirm that monitoring of radiation workers should not only be solely based on physical dosimetry, but also on the biological indicators, which have the advantage of measuring the individual radiation damage.
Keywords: Protein P53 expression, Ionizing radiation, Cell damage.
