the effect of different levels of nano zinc and

(1)

١

توﺎﻔﺘﻣ حﻮﻄﺳ ﺮﺛا ﯽﺳرﺮﺑ يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ

، ﻦﻫآ ﺪﯿﺴﮐاﻮﻧﺎﻧ ﻪﻧﻮﻤﻧﺰﯾر و

لﺎﮐﺮﺑ ﻒﻠﺘﺨﻣ يﺎﻫ ﯽﯾاز

و هﺪﻧز

سﻮﻟﺎﮐ ﯽﻧﺎﻣ ﻞﮔ

ﻦﯾا ﻂﯾاﺮﺷ رد ﯽﻋار وﺮﺘﯾو

ا ﯽﻓﺮﺷ ﻢﯿﻫاﺮﺑ

*

ﻮﻠﻨﺴﺣ هﺮﻫﺎﻃ ،1

،3

نﺎﯿﮐﻮﺘﻓ ﻦﯿﺴﺣﺪﻤﺤﻣ ﯽﯾﻮﮑﻧ مﺎﯿﺧ ﯽﺒﺘﺠﻣ ﺪﯿﺳ ،2

يدواد شﻮﯾراد ،3 3

1 - ﺸﮐ يژﻮﻟﻮﻨﮑﺗﻮﯿﺑ ﺪﺷرا ﯽﺳﺎﻨﺷرﺎﮐ يﻮﺠﺸﻧاد يزروﺎ

،يزروﺎﺸﮐ هﺪﮑﺸﻧاد ، ﺪﻫﺎﺷ هﺎﮕﺸﻧاد

2 - ﻮﻀﻋ ﻫﯿ ﺪﻫﺎﺷ هﺎﮕﺸﻧاد ،يزروﺎﺸﮐ هﺪﮑﺸﻧاد ،ﯽﻤﻠﻋ ﺖﺌ

3 - ﻮﻀﻋ ﻫﯿ ناﺮﯾا يزروﺎﺸﮐ يژﻮﻟﻮﻨﮑﺗﻮﯿﺑ هﺪﮑﺸﻫوﮋﭘ ﯽﻤﻠﻋ ﺖﺌ

*[email protected]

هﺪﯿﮑﭼ ﻞﮔ ﻣ صاﻮﺧ ياراد ﻦﯾرﻮﻓﺮﭙﯾﺎﻫ و ﻦﯿﺳﺮﭙﯾﺎﻫ ﯽﻨﻌﯾ نآ ﺮﺛﻮﻣ داﻮﻣ ﻪﮐ ﺖﺳا ﻢﻬﻣ ﯽﯾوراد هﺎﯿﮔ ﮏﯾ ﯽﻋار ﺖﯿﺻﺎﺧ ﺪﻨﻧﺎﻣ ﯽﻤﻬ

ﺪﺿ ﺪﺿ ،ﯽﮔدﺮﺴﻓا ﺪﺿ و ﯽﺳوﺮﯾو

ﯽﻣ يﺮﺘﮐﺎﺑ ﯽﻧاواﺮﻓ دﺮﺑرﺎﮐ ﯽﯾوراد ﻊﯾﺎﻨﺻ رد و ﺪﺷﺎﺑ

دراد . ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﺮﺛا ﻖﯿﻘﺤﺗ ﻦﯾا رد

توﺎﻔﺘﻣ

ﻦﻫآ ﺪﯿﺴﮐاﻮﻧﺎﻧ و يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ و

ﺰﯾر عﻮﻧ سﻮﻟﺎﮐ ﺮﺑ ﻪﻧﻮﻤﻧ ﯽﯾاز

و ﯽﻧﺎﻣ هﺪﻧز ﯽﻋار ﻞﮔ سﻮﻟﺎﮐ

ﻞﯾرﻮﺘﮐﺎﻓ ﺶﯾﺎﻣزآ ﺐﻟﺎﻗ رد

ﮐ ﻪﯾﺎﭘ حﺮﻃ ﺎﺑ ﺎﺑ ﯽﻓدﺎﺼﺗ ﻼﻣﺎ

5 و راﺮﮑﺗ 5 ﺰﯾر راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﻪﻧﻮﻤﻧ ﺖﻓﺮﮔ

. ﻪﺸﯾرو گﺮﺑ ،ﻪﻗﺎﺳ ﻞﻣﺎﺷ هدﺎﻔﺘﺳا درﻮﻣ يﺎﻫ ﻪﻧﻮﻤﻧ ﺰﯾر

ﻂﯿﺤﻣ يورﺮﺑ ﻪﮐ ﺖﺳا

MS

ﻦﻫآ ﺪﯿﺴﮐا ﻮﻧﺎﻧ يوﺎﺣ )

،ﺮﻔﺻ 25 ، 50 ، 75 ، 100 ﯽﺑ ﯽﭘ ﯽﭘ (

يور ﺪﯿﺴﮐا ﻮﻧﺎﻧو )

،ﺮﻔﺻ 25 ، 50 ، 75 ،

100 ﯽﺑ ﯽﭘ ﯽﭘ (

ﺖﻓﺮﮔ راﺮﻗ .

ﻪﻧﻮﻤﻧ ﺖﺸﮐ يﺎﻫ

هﺪﺷ يﺎﻣد رد ﯽﮑﯾرﺎﺗ رد 23

رد ﻪﺟ ﯽﺘﻧﺎﺳ داﺮﮔ ﺪﻧﺪﺷ يراﺪﻬﮕﻧ .

ﺖﺷﺬﮔ زا ﺲﭘ 28

زور

ﻪﻧﻮﻤﻧ ﺰﯾر عﻮﻧ ﻦﯿﺑ ﻞﺑﺎﻘﺘﻣ ﺮﺛا و

توﺎﻔﺘﻣ حﻮﻄﺳ ﻦﻫآ ﺪﯿﺴﮐاﻮﻧﺎﻧ و يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ

راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﺖﻓﺮﮔ

. ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻦﯿﺑ

زو ﯽﯾاز لﺎﮐ ﺮﻈﻧ زا ﺪﺻ رد ﮏﯾ ﺢﻄﺳ رد راد ﯽﻨﻌﻣ ﺮﺛا ﻪﻧﻮﻤﻧ ﺰﯾر عﻮﻧو داﻮﻣ ﺪﺷ هﺪﻫﺎﺸﻣ سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧ

. ناﺰﯿﻣ ﺮﺑ ار ﺮﺛا ﻦﯾﺮﺘﺸﯿﺑ

ﺖﻈﻠﻏ ﺎﺑ يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز و ﯽﯾاز سﻮﻟﺎﮐ 100

ﺖﻈﻠﻏ ﺎﺑ ﻦﻫآ ﺪﯿﺴﮐا ﻮﻧﺎﻧ ار ﺮﺛا ﻦﯾﺮﺘﻤﮐو ﯽﺑ ﯽﭘ ﯽﭘ 100

ﯽﺑ ﯽﭘ ﯽﭘ

ﺖﺷاد ﻪﻧﻮﻤﻧ ﺰﯾر عﻮﻧ ﻪﺳ ﺮﻫ ياﺮﺑ .

هژاو يﺪﯿﻠﮐ يﺎﻫ :

ﻞﮔ

،ﯽﻋار اﻮﻧﺎﻧ ،سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز ،ﯽﯾاز لﺎﮐ ﻦﻫآ ﺪﯿﺴﮐاﻮﻧﺎﻧ ،يور ﺪﯿﺴﮐ

.

(2)

٢

ﻪﻣﺪﻘﻣ

ﻪﻌﻟﺎﻄﻣ او ﮐ ﺶﻨ ﮐ لﺎ از ﯽﯾ ناﻮﻨﻋ ﻪﺑ سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧزو ﻘﺤﺗ

ﯿ تﺎﻘ ﻨﺑ ﯿ دﺎ ي و ﺎﭘﯾ ﻪ ﻪﺑ رﻮﻈﻨﻣ تﺎﻌﻟﺎﻄﻣ ﺪﻌﺑ

ي لﺎﻘﺘﻧا نژ و ﻘﺤﺗ ﯿ تﺎﻘ

ﺑﯿ ﺗﻮ ﮑ ژﻮﻟﻮﻨ ي روﺮﺿ ي ﺖﺳا )

،يﺰﯾﺰﻋ 1381 ( . ﻦﯾا رد ﺶﻫوﮋﭘ يور ﺪﯿﺴﮐا و ﻦﻫآ ﺪﯿﺴﮐا داﻮﻣ ﻮﻧﺎﻧ ود ﺮﺛا رﺎﺑ ﻦﯿﻟوا ياﺮﺑ ناﺰﯿﻣ ﺮﺑ

ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﯽﻋار ﻞﮔ رد سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز و ﯽﯾاز سﻮﻟﺎﮐ .

داﻮﻣ و ﺎﻬﺷور

ﺪﺷ ﻪﯿﻬﺗ ﺪﻫﺎﺷ هﺎﮕﺸﻧاد ﯽﯾوراد نﺎﻫﺎﯿﮔ تﺎﻘﯿﻘﺤﺗ ﺰﮐﺮﻣ رﺬﺑ ﮏﻧﺎﺑ زا ﯽﻋار ﻞﮔ يﺎﻫ رﺬﺑ .

تﺪﻣ ﻪﺑ ﺎﻫرﺬﺑ 20

ﻪﻘﯿﻗد ﺖﯾﺮﻠﮐﻮﭙﯿﻫرد

ﻢﯾﺪﺳ 5 ﺲﭙﺳو ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﺪﺻرد 3

ﻪﺒﺗﺮﻣ ﺎﺑ بآ ﺮﻄﻘﻣ ﺮﺘﺳا ﯾ ﻪﺑ ﻞ تﺪﻣ 12 ﺪﻧﺪﺷ هداد ﻮﺸﺘﺴﺷ ﻪﻘﯿﻗد .

ﺮﯾز رد هﺪﺷ ﻪﺘﺴﺷ يﺎﻫ رﺬﺑ

تﺪﻣ ﻪﺑ ﻞﯾﺮﺘﺳا ﻼﻣﺎﮐ دﻮﻫ 60

ﺪﻧﻮﺷ ﮏﺸﺧ ﻼﻣﺎﮐ ﺎﺗ ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻪﻘﯿﻗد .

ﻂﯿﺤﻣ يوﺮﺑ نﺪﺷ ﮏﺸﺧ زا ﺪﻌﺑ ﺎﻫ رﺬﺑ يوﺎﺣMS

30 مﺮﮔ

و زرﺎﮐﺎﺳ 5/

7 ﺎﺑ رﺎﮔآ مﺮﮔ 7/PH

5 يرﻮﻧ ﻂﯾاﺮﺷﺎﺑ نوﺮﺗﻮﺘﯿﻓ رد و ﺪﻨﺘﻓﺮﮔ راﺮﻗ 2400

ﻮﻧ هرود ، ﺲﮐﻮﻟ ير

16 و ﯽﯾﺎﻨﺷور ﺖﻋﺎﺳ 8

يﺎﻣد رد ﯽﮑﯾرﺎﺗ ﺖﻋﺎﺳ 2

+ 25 ﺪﻧﺪﺷ يراﺪﻬﮕﻧ .

زاﺪﻌﺑ 40 ﻪﺑو ﺪﻧﺪﺷ اﺪﺟ ﻪﻗﺎﺳو ﻪﺸﯾر، گﺮﺑ يﺎﻫ ﻪﻧﻮﻤﻧ ﺰﯾر ﺖﺳر ﻪﻧاد ﺮﻫ زا زور

ﻂﯿﺤﻣ MS ﻦﻫآ ﺪﯿﺴﮐا ﻮﻧﺎﻧ يوﺎﺣ )

،ﺮﻔﺻ 25 ، 50 ، 75 ، 100 ﯽﺑ ﯽﭘ ﯽﭘ (

يور ﺪﯿﺴﮐا ﻮﻧﺎﻧو )

،ﺮﻔﺻ 25 ، 50 ، 75 ، 100 ﯽﺑ ﯽﭘ ﯽﭘ (

ﺪﻨﺘﻓﺎﯾ لﺎﻘﺘﻧا .

هدﺎﻔﺘﺳا درﻮﻣ نﻮﻣرﻮﻫ 2-4,D

ﺖﻈﻠﻏ ﺎﺑ 2

وﺮﺘﯿﻟ ﺮﺑ مﺮﮔ ﯽﻠﯿﻣ ﺖﻈﻠﻏ ﺎﺑ BAP

8/

0 دﻮﺑ ﺮﺘﯿﻟﺮﺑ مﺮﮔ ﯽﻠﯿﻣ .

12 ﯽﻠﯿﻣ

رد ﺖﺸﮐ ﻂﯿﺤﻣ ﺮﻫ زا ﺮﺘﯿﻟ 5

دﺎﻌﺑا ﺎﺑ يا ﻪﺸﯿﺷ يﺎﻫ ﺶﯾد يﺮﺘﭘ ﻞﺧادراﺮﮑﺗ 8/

1

* 6 فﺮﻇ ﺮﻫردو ، ﻊﯾزﻮﺗ ﺮﺘﻣ ﯽﺘﻧﺎﺳ 5

ماﺪﻧا زا ﻪﻌﻄﻗ

ﺪﺷ هداد ﺖﺸﮐ ﺮﻈﻧ درﻮﻣ .

ﺎﺗ ﻂﯾاﺮﺷ رد ﺎﻫ ﻪﻧﻮﻤﻧ يﺎﻣد رد ﯽﮑﯾر

23 ﺪﻨﺘﻓﺮﮔ راﺮﻗ داﺮﮔ ﯽﺘﻧﺎﺳ ﻪﺟرد .

ﺖﺷﺬﮔ زا ﺲﭘ 28

ﺮﺛا زور

و ﻪﻧﻮﻤﻧ ﺰﯾر عﻮﻧ ﻦﯿﺑ ﻞﺑﺎﻘﺘﻣ حﻮﻄﺳ

توﺎﻔﺘﻣ ﻦﻫآ ﺪﯿﺴﮐاﻮﻧﺎﻧ و يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ

راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﺖﻓﺮﮔ

.

ﺞﯾﺎﺘﻧ يرﺎﻣآ ﯽﺑﺎﯾزرا سﻮﻟﺎﮐ هزاﺪﻧا :

ﺑ يﺎﻫ هداد و ﺖﻓﺮﮔ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ هﺪﻧﺎﻣ هﺪﻧز يﺎﻫ سﻮﻟﺎﮐ داﺪﻌﺗو هﺪﻣآ ﺖﺳﺪ

ﺐﻟﺎﻗ رد

ﯽﻓدﺎﺼﺗ ﻼﻣﺎﮐ ﻪﯾﺎﭘ حﺮﻃ ﺎﺑ ﻞﯾرﻮﺘﮐﺎﻓ ﺶﯾﺎﻣزآ راﺰﻓا مﺮﻧ ﺎﺑ

ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﺰﯿﻟﺎﻧآ درﻮﻣSPSS

.

ﺞﯾﺎﺘﻧ

ﺑﯿ ﻦ حﻮﻄﺳ ﺗﯿ رﺎﻤ ﺎﻫ ي ﻦﻫآ ﺪﯿﺴﮐاﻮﻧﺎﻧ و يورﺪﯿﺴﮐا ﻮﻧﺎﻧ و

ﻪﻧﻮﻤﻧ ﺰﯾر عﻮﻧ ﻦﯿﻨﭽﻤﻫ ﺮﺛا ﻨﻌﻣ توﺎﻔﺗ

ﯽ رد راد ﺢﻄﺳ 1 ﺪﺻرد هزاﺪﻧا ﺮﻈﻧ زا

هﺪﻧز يﺎﻫ سﻮﻟﺎﮐ داﺪﻌﺗو سﻮﻟﺎﮐ ﻫﺎﺸﻣ

هﺪ ﺪﺷ .

يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﺖﻈﻠﻏ رد :

100 ﯽﺑ ﯽﭘ ﯽﭘ سﻮﻟﺎﮐ هزاﺪﻧا ناﺰﯿﻣﺮﺑ ﺮﺛا ﻦﯾﺮﺘﺸﯿﺑ

) 5 ﺮﺘﻤﯿﻠﯿﻣ ( سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز و )

5 دﺪﻋ ( ﻪﺳﺮﻫ ياﺮﺑ

ﺪﺷ هﺪﻫﺎﺸﻣ ﻪﻧﻮﻤﻧ ﺰﯾر عﻮﻧ

.

يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ رد ﻪﻗﺎﺳ ﻪﻧﻮﻤﻧ ﺰﯾر - 1ﻞﮑﺷ

(3)

٣

يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ ردگﺮﺑ ﻪﻧﻮﻤﻧ ﺰﯾر- 2ﻞﮑﺷ

يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ ردﻪﺸﯾر ﻪﻧﻮﻤﻧﺰﯾر -3ﻞﮑﺷ

ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻦﻫآ

ﺖﻈﻠﻏ رد: 100

ﯽﺑ ﯽﭘ ﯽﭘ ياراد

سﻮﻟﺎﮐ هزاﺪﻧا ناﺰﯿﻣﺮﺑ ﺮﺛا ﻦﯾﺮﺘﻤﮐ )

ﺮﺘﻤﯿﻠﯿﻣ ﺮﻔﺻ (

سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز و )

0 ( ياﺮﺑ

ر ﻪﻧﻮﻤﻧ ﺰﯾ ﻪﺸﯾرو گﺮﺑ ﺪﺷ هﺪﻫﺎﺸﻣ

.

ﻦﻫآﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ ردگﺮﺑﻪﻧﻮﻤﻧ ﺰﯾر - 4ﻞﮑﺷ

(4)

٤

ﻦﻫآﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ رد ﻪﺸﯾرﻪﻧﻮﻤﻧ ﺰﯾر - 5ﻞﮑﺷ

ﻞﮑﺷ 6 -

ﻦﻫآﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ رد ﻪﻗﺎﺳ ﻪﻧﻮﻤﻧ ﺰﯾر

يﺮﯿﮔ ﻪﺠﯿﺘﻧو ﺚﺤﺑ

ﺪﯿﺴﮐا هرد ﻮﻧﺎﻧ ود ﺮﺛا رﺎﺑ ﻦﯿﻟوا ياﺮﺑ ﺶﻫوﮋﭘ ﻦﯾا رد ﺪﻧا هﺪﺷ ﺖﺸﮐ ﻂﯿﺤﻣ دراو ﻪﮐ ﺪﯾﺪﺟ يﺎﻫ رﻮﺘﯿﺴﯿﻟا ناﻮﻨﻋ ﻪﺑ ﻦﻫآ ﺪﯿﺴﮐاو يور

ﺪﺷ ﯽﺳرﺮﺑ .

ا ،ﺪﻨﺷﺎﺑ ﯽﻣ راﺬﮔ ﺮﺛا ﻪﯾﻮﻧﺎﺛ يﺎﻫ ﺖﯿﻟﻮﺑﺎﺘﻣ ﺪﯿﻟﻮﺗ ناﺰﯿﻣﺮﺑ دﺎﯾز رﺎﯿﺴﺑ ناﺰﯿﻣ ﻪﺑ ﯽﻄﯿﺤﻣ ﻞﻣاﻮﻋ ز

ﺮﺻﺎﻨﻋ ﻞﻣاﻮﻋ ﻦﯾا ﻪﻠﻤﺟ

ﺪﻨﺘﺴﻫ يورو ﻦﻫآ ﺪﻨﻧﺎﻣ ﻦﯿﮕﻨﺳ .

ﻗ ﻪﮐ دراد ﻖﻠﻌﺗ نﺎﻫﺎﯿﮔ زا ﯽﻫوﺮﮔ ﻪﺑ ﯽﻋار ﻞﮔ يﺎﻫ ﺖﻈﻠﻏ رد ار يورو مﻮﯿﻣدﺎﮐ ﺮﯾدﺎﻘﻣ ﺖﺳا ردﺎ

ﺪﻨﮐ ﻪﺘﺷﺎﺒﻧا دﻮﺧ يﺎﻫ ﺖﻓﺎﺑ رد ﻻﺎﺑ )

Kralova et al, 2003 .(

ﺮﯿﺛﺎﺗ ﻦﯾﺮﺘﺸﯿﺑ ياراد يور ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﻪﮐ ﺪﺷﺎﺑ ﻞﯿﻟد ﻦﯿﻤﻫ ﻪﺑ ﺪﯾﺎﺷ

ﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز و ﯽﯾاز سﻮﻟﺎﮐ ناﺰﯿﻣﺮﺑ ار ﺖﺳا س

. ﺖﻈﻠﻏ رد ﻦﻫآ ﺪﯿﺴﮐا ﻮﻧﺎﻧ ﺮﮕﯾد فﺮﻃ زا 100

اد ﯽﺑ ﯽﭘ ﯽﭘ ﺮﺛا ﻦﯾﺮﺘﺸﯿﺑ يار

سﻮﻟﺎﮐ ﯽﻧﺎﻣ هﺪﻧز و ﯽﯾاز سﻮﻟﺎﮐ ناﺰﯿﻣﺮﺑ ﺮﺑ ﯽﻔﻨﻣ ﺖﺳاراد ار

. ﻮﯿﺑ يﺎﻫ ﺮﯿﺴﻣ رد ﺖﻟﺎﺧد ﻞﯿﻟد ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ ﺞﯾﺎﺘﻧ ﻦﯾا ﻪﺘﺒﻟا

دراد ﺮﺘﺸﯿﺑ ﻖﯿﻘﺤﺗ و ﻪﻌﻟﺎﻄﻣ ﻪﺑ زﺎﯿﻧ ﻪﮐ ﺪﺷﺎﺑ ﺖﺸﮐ ﻂﯿﺤﻣ ﺎﯿﺻﻮﺼﺧو ﯽﯾﺎﯿﻤﯿﺷ .

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ﻊﺑﺎﻨﻣ : 1.Azizi, M., Omidbeigi, R. 2002. The investigation of Hypericum perforatum L .in the in vitro condition in production of hypericin and other secondary metabolites. Pajouhesh and sazandegi journal. No: 40, 15-45.

2.Kralova, K., and Masarovicova, E. 2003.Hypericum perforatum L. and Chamomilla re cutita(L.) Rausch.-accumulators of some metals. Pharmazie.58:359-360.

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The effect of different levels of nano zinc oxid ,nano iron oxid and different explants on the callogenesis and callus survival of Hypericum perforatum L. in vitro

E.SHARAFI^1*,T.HASANLOO³,M.H.FOTOKIAN²,S.M.KHAYYMNKYY³,D.DAVOODI³ 1-Agricultual biotechonology graduate student, Faculity of Agriculture, Shahed University 2-Academic member, Faculity of Agriculture, Shahed University

3-Academic member Agricultural Biotechnology Researh Institute of Iran

*[email protected] Abstract

St John's wort (Hypericum perforatum L.) is an important medicinal plant which contains effective materials such as hypericin and hyperforin. These materials demonstrate as an anti-depressant, antiviral and antibacterial agents and widely use in the pharmaceutical industry. In this study the effect of different levels of nano zinc oxide and nano iron oxide and explants type on callogenesis and callus survival were examined with factorial experiment design on the basis of completely randomized design with five repeats and five explants. The root, stem and seedlings leave of sterile plantlets were used as a explants to culture on MS medium containing nano-iron oxide (zero, 25, 50, 75, 100 ppb) and nano-zinc oxide (zero, 25, 50, 75, 100 ppb). The grown samples were put in the growth chamber in the darkness condition at 23 °C. After 28 days, the interaction between different type’s explants and levels different nano zinc oxide and nano iron oxide was investigated. The significant effect at 1 percent probability was observed between different levels of nano-materials and explants type for callogenesis and callus survival. The media contained nano zinc oxide with the concentration of 100 ppb affect on the callogenesis and callus survival significantly and the media contained nano iron oxide with the concentration of 100 ppb had the lowest effect followed by each three explants.

Key words: Hypericum perforatum L, callogenesis, callus survival, nano zinc oxid , nano iron oxid.