The 17th National & 5th International Iranian Biology Cnference Septamber 4-6, 2012 Shahid Bahonar University of Kerman
Determination of Rosmarinic Acid in Some Iranian Salvia Species by HPLC method
Roghayehsadat Ejtahed1*, Tayebeh Radjabian1, Sayed Ali Hoseini Tafreshi2, Asghar Kamrani1
1BiologyDepartment, Shahed University, Tehran, Iran
2BiologyDepartment, University of Kashan, Kashan, Iran E-mail: [email protected]
Rosmarinic acid (RA) is a biologically active phenolic compound, which commonly found in species of the Boraginaceae and Lamiaceae families. To the best of our knowledge, among 58 Iranian native Salvia species, only S.limbata has been studied for the prescence of rosmarinic. The objective of this paper was determination of rosmarinic acid content in the different organs of four Salvia species of Iran by HPLC method. Hydroalcoholic extracts of the dried and powdered samples were prepared for analysis of RA. Based on our results, among the studied organs, leaves were the richest sources of RA. The highest amounts of RA were determined in leaves of S. officinalis followed by S. sclarea (24.13 ± 0.99 and 11.49 ± 0.89 mg/g dry weight). Among the stem organs, the highest contents of RA were found in stems of S. officinalis and S. reuterana (10.71 ± 0.24 and 6.24 ± 0.07 mg/g dry weight). Root samples of S. officinalis and S. sclarea with 6.51 ± 0.46 and 4.04 ± 0.37 mg/g dry weight, respectively had the highest contents of RA. In conclusion, based our results, some Iranian Salvia species could be introduced as potent sources of RA.
Key Word: Lamiaceae ,Salvia species, Rosmarinic acid, HPLC method
ناﺮﻳاﻲﺳﺎﻨﺷﺖﺴﻳزﻲﻠﻠﻤﻟاﻦﻴﺑﺲﻧاﺮﻔﻨﻛﻦﻴﻤﺠﻨﭘويﺮﺳاﺮﺳﺲﻧاﺮﻔﻨﻛﻦﻴﻤﻫﺪﻔﻫ 16
- رﻮﻳﺮﻬﺷ14 نﺎﻣﺮﻛﺮﻨﻫﺎﺑﺪﻴﻬﺷهﺎﮕﺸﻧاد91
رد ﺪﻴﺳا ﻚﻴﻨﻳرﺎﻣزور ﺶﺠﻨﺳ ﻲﺧﺮﺑ
ﻲﻠﮔ ﻢﻳﺮﻣ يﺎﻫ ﻪﻧﻮﮔ ناﺮﻳا
شور ﻪﺑ ﻊﻳﺎﻣ ﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛ
ناﻮﺗﺮﭘ
ﺳﻪﻴﻗر ﺪﻬﺘﺟاتادﺎ
1
نﺎﻴﺒﺟرﻪﺒﻴﻃ،*
ﻲﻨﻴﺴﺣﻲﻠﻋﺪﻴﺳ،1
ﻲﻧاﺮﻣﺎﻛﺮﻐﺻا،2 1
ﻲﺳﺎﻨﺷﺖﺴﻳزهوﺮﮔ1
، ناﺮﻳا،ناﺮﻬﺗ،ﺪﻫﺎﺷهﺎﮕﺸﻧاد
هوﺮﮔ2 ﻲﺳﺎﻨﺷﺖﺴﻳز
، ناﺮﻳا،نﺎﺷﺎﻛ،نﺎﺷﺎﻛهﺎﮕﺸﻧاد
E-mail: [email protected]
ﻚﻴﻨﻳرﺎﻣزر ﺪﻴﺳا
ﺐﻴﻛﺮﺗ ﻲﻠﻨﻓ ﺖﺴﻳز ﻟﺎﻌﻓ ﻲ ﺖﺳا ﻪﻛ لﻮﻤﻌﻣرﻮﻄﺑ رد
يﺎﻫﻪﻧﻮﮔ هﺮﻴﺗ
يﺎﻫ ﮔ ﺑزوﺎ نﺎﻴﻧﺎ ﺖﻓﺎﻳنﺎﻴﻋﺎﻨﻌﻧو دﻮﺷﻲﻣ
.
زا ﻲﻠﮔ ﻢﻳﺮﻣ ﻪﻧﻮﮔ 58 ssp.)
Salvia رد ﻪﺘﻓﺎﻳ ﺶﻳور (
،ناﺮﻳا دﻮﺟو ﺪﻴﺳا ﻚﻴﻨﻳرﺎﻣزر ﺎﻬﻨﺗ
ﻲﻠﮔ ﻢﻳﺮﻣ رد راد ﻪﺒﻟ
) S. limbata راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ (
ﺖﺳا ﻪﺘﻓﺮﮔ زا فﺪﻫ .
ﺮﺿﺎﺣ ﺶﻫوﮋﭘ ياﻮﺘﺤﻣ ﻦﻴﻴﻌﺗ
ﻚﻴﻨﻳرﺎﻣزر ﺪﻴﺳا
رد ماﺪﻧا ﻲﻠﮔﻢﻳﺮﻣﻪﻧﻮﮔرﺎﻬﭼﻒﻠﺘﺨﻣيﺎﻫ ناﺮﻳا
دﻮﺑ زا . هرﺎﺼﻋ يﺎﻫ ﺎﻫﻪﻧﻮﻤﻧﻲﻠﻜﻟاورﺪﻴﻫ ي
ردﻮﭘو ﻚﺸﺧ هﺪﺷ
ياﺮﺑ ﺶﺠﻨﺳ
ﺪﻴﺳا ﻚﻴﻨﻳرﺎﻣزر ﻪﺑ
ناﻮﺗﺮﭘ ﻊﻳﺎﻣ ﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛ شور HPLC
)
هدﺎﻔﺘﺳا ( ﺷ ﺪ
،هﺪﻣآ ﺖﺳﺪﺑ ﺞﻳﺎﺘﻧ سﺎﺳا ﺮﺑ . اﺪﻘﻣ ﻦﻳﺮﺘﺸﻴﺑ
ر
ﻚﻴﻨﻳرﺎﻣزر ﺪﻴﺳا
گﺮﺑرد يﺎﻫ ﻲﻳورادﻲﻠﮔﻢﻳﺮﻣ )
icinalis S. off
و ( ﻲﻠﮔﻢﻳﺮﻣ S. sclarea)
نﺎﺳاﺮﺧﺖﻴﻌﻤﺟ ( )
±0,99
24,13 و
±0,89 11,49 مﺮﮔ ﻲﻠﻴﻣ ﻚﺸﺧنزومﺮﮔﺮﺑ/
ﺪﻣآﺖﺳﺪﺑ(
،ﺎﻫ ماﺪﻧاﺮﻳﺎﺳﻦﻴﺑ رد. ﻪﻗﺎﺳ
ﻢﻳﺮﻣو ﻲﻳورادﻲﻠﮔﻢﻳﺮﻣ
ﻲﻧﺎﻬﻔﺻاﻲﻠﮔ reuterana)
S.
( )
±0,24 10,71 و
±0,07 مﺮﮔﻲﻠﻴﻣ6,24
ﻚﺸﺧنزومﺮﮔﺮﺑ/ و (
ﻪﺸﻳر ﻳﺮﻣ ﻲﻳورادﻲﻠﮔﻢ و
ﻲﻠﮔﻢﻳﺮﻣ )
±0,46 و6,51
±0,37 مﺮﮔﻲﻠﻴﻣ4,04
ﻚﺸﺧنزومﺮﮔ ﺮﺑ/ ﺪﻨﺘﺷادارﺪﻴﺳاﻚﻴﻨﻳرﺎﻣزرياﻮﺘﺤﻣﻦﻳﺮﺘﺸﻴﺑ (
ﺎﺑ.
ناﻮﺗﻲﻣ،ﺞﻳﺎﺘﻧﻪﺑﻪﺟﻮﺗ ﻲﺧﺮﺑ
يﺎﻫﻪﻧﻮﮔ ﻲﻠﮔﻢﻳﺮﻣ
ﻊﺑﺎﻨﻣناﻮﻨﻋﻪﺑارناﺮﻳا زاﻲﻨﻏ
ﻚﻴﻨﻳرﺎﻣزر ﺪﻴﺳا
دﻮﻤﻧﻲﻓﺮﻌﻣ .
يﺪﻴﻠﻛتﺎﻤﻠﻛ
،نﺎﻴﻋﺎﻨﻌﻧ:
،ﺪﻴﺳاﻚﻴﻨﻳرﺎﻣزر،ﻲﻠﮔﻢﻳﺮﻣ ﻊﻳﺎﻣﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛ
ناﻮﺗﺮﭘ
