Tap chl Y Duvc hqc - Truimg Dqi hqc Y Duvc Hui- Tgp 6, si4 - thdng 8/2016
TRICH LY BQT LYCOPENE TLT GAC HUOlMG DEN O'NG DUNG TRONG THirC PHAM CHLTC NANG VA MY PHAM
Nguyen Thj Li Thuy'^, Do Thanh Sinh', Tran Thj Hda' (1) Trung tam Nghien cUu va Trien khai, khu Cong nghe cao Thdnh pho Ho ChiMinh
12) Khoa Co ban, Trubng Bgi hgcY Dugc Hue - Dpi hgc Hue Tdm tat
Dat van de: Gae la trai ciy ch&a nhieu chat ed feh vdi ham lu'png cao, dgc bigt l i lycopene, mpt logi caro- tenoid chdng oxy hoi mgnh. Tuy vgy nh&ng san pham t& trai gac van han chl v l sy da dgng va gia trj. Chiing tdi trinh bay qui trinh trich ly bdt lycopene v i |3-carotene t y dau gac de cd the md rpng &ng dyng eho thye phim ch&c nang va my phim. Odi tu'png v i phuang phip nghien c&u: Khao sat qua trinh trich ly theo cac thdng sd anh hudng din higu suat va chi gidi ban phyang phap xa phdng hoa dau vdi cic hoi chat thin thien la ethanol, propylene glycol, KOH, NaCl. Kit qua: Him lu'png hai thanh phan chinh l i lycopene va p-carotene xic dinh dong thdi bang phuang phap phd UV-Vis eho thay higu suat ciia qui trinh dgt d i n 65,07% khi s&
dyng 8 g KOH 12 mol/L d l xa phdng hoi 20g dau gae trong 120 phiit. Do tinh khilt ciia san pham dgt 89,02%.
Ket luan: Quy trinh dgt higu suat kha cao va tdi thieu hoi chat ed hgi trong san pham. Ngoai ra phuang phap cijng ch&ng minh dupe ti'nh dn djnh de ed the sin xuat b quy md pilot.
Tir khoa: Carotenoids, lycopene, trich ly an todn, UV Vis, xdc dinh thdnh phdn dong thbi.
Abstract
LYCOPENE POWDER PRODUCING FROM GAC FRUIT TOWARDS APPLICATIONS IN NUTRACEUTICAL AND COSMETICS
Nguyen Thi Le Thuy'', Do Thanh Sinh', Tran Thi Hoa' (1) R&D labs, Saigon High-tech Park, Ho Chi Minh City (2) faculty of Basic Science Department, Hue University of Medicine and Pharmacy - Hue University Backgroud: Gac is a fruit containing many antioxidants, especially lycopene, with high concentration.
However the variety and value of products from gac are limited. We introduce a modified method to produce lycopene and others carotenoids from gac oil tovwards available and safe applications in nutraceutical and cosmetics. Materials and method: We study the extraction with different parameters and limit at the saponification of gac oil using less toxic substances such as ethanol, propylene glycol, postasium hydroxide and sodium chlorid. Results: Concentrations of lycopene and P-carotene determined silmutaneously by UV- Vis spectrophotometer present that the efficiency reaches 65.07% as 20 g of gac oil is saponified with 8 g KOH 12 mol/L in 120 mins. The purity of product is 89.02%. Conclusions: This process could minimize toxic residue in the powder after the precipitati'on, filtration and washing. Besides, the method is stable and applicable to the mass production.
Keywords: Carotenoids, Lycopene, safe extraction, UV Vis silmutaneous concentration determination.
1. MOroXu
Lycopene l i mpt trong nh&ng hogt chat ran cd oxy m&c dan d nang lu'png cao. Mpt sd cdng dung miu dd dgm die trung, cd dge tinh khang oxy hoi chinh ciia lycopene du'pc ch&ng mmh trong y van rat cao trong gan 600 logi carotenoids dupc biet nhU kha nang ngan ng&a lao hoa, bgnh nhdl mau den. Dac ti'nh nay giup lycopene cd the bao ve cP ca tim va ung thu tien ligt tuyin, khi ning cai thign the eon ngudi khdi cac bgnh t i t ve suy thoai lam ding k l tl Ig tinh triing v i mang thai thanh edng ciia thay ddi ADN bang cich trung hoi cac gdc t y do va eae cap hilm mudn.
-a....-..-^^: t^^py^n ThiL0 Thuy, email: ti.thuy.nguyen^gmaii,
%l^«'Sl£ 3/8/2016: Ngay ddng y dang: 12/9/2016; Ngdyn
JOURNAL OF MEDICINE AND
rp ch! Y Duac hgc - TruVng Dgi hgc Y Dugc Hue- Tgp 6, so 4 - thang 8/2016 Ben eanh nh&ng cdng dyng eiia thyc pham ch&c nang, lycopene cdn duae &ng dyng nhieu trong my phim nh&ng nam qua. Thyc vgy, vl lycopene la mpt chudi hydrocarbon md eiia earotenoid chua bao hoa vdi 11 ndi ddi lien hpp, nd hogt dpng nhu mdt chat ehdng lai cic tie nhin oxy hoa eua mdi trudng, tia UV va b&c xg cd budc sdng dai eua anh sang kha kien. Khi phdi hpp trong my phim du&ng da, nd ed the bao ve hogc lam giam anh hudng ngan hgn (chay nang) va dai han (ung thu da) eiia inh sing mgt trdi.
Trong t y nhien, thdng thudng lycopene duac tim thay trong cic loai t r i i ciy va rau cii phd bien cd mau dd din ving nhu ci chua, c i rdt, dua hau, du dil. Chi die biet trong t r i i glc, lycopene co ndng dp cao han nhieu so vdi cic logi trai ciy khac, va tgp trung nhieu b mang dd bao quanh hgt gac (2073 mg/kg). Phan thit qua gac eung ch&a earotenoid nhung vdi ndng dp thap han. Mgc dii khdi lupng mang gae tuai ehi ehiem khoang 24,6% khdi lupng qua gae nhung vdi ham lupng lycopene gap t& 50 - 200 lan ham lupng d ca chua (42 mg/kg) [1], vigc trich ly lycopene t& gae dupe quan tam nghien e&u ngay eang nhieu. Trong bai nay chung tdi trinh bay phuang phap trich ly bpt lycopene t& dau gac de t&
dd cd the &ng dyng vao s i r pham khae nhau.
Mang do hgt gac ed khoang 85,57% nuPc, 6,92% ehat beo, 3,93% carbohydrate, 1,95% pro- tein, 0,83% spi va 0,80% tro. Sau khai tich mang ra khdi hat va xay nhuyln, cd hai phyang phap chinh de trich ly lycopene l i x i phdng hoi ming tuai rdi tich bang dung mdi h&u ca, hoge ep het dau roi sau dd x i phdng hoi va ipc. Vdi myc dieh &ng dyng sin pham mdt cich an toan trong thyc pham ch&c nang va hgn che du lupng hoi chat h&u eO cdn lai trong san pham, chung tdi lya chpn phuang phap th& hai. Ngoai ra nghien c&u ehi gidi hgn khao sat qui trinh trich iy lycopene t& dau gac d cic dieu kign khic nhau.
2. VAT LIEU VA PHiraNG PHAP 2.1. Vit lieu thi nghigm
Cic hoa chat diing cho qui trinh xa phdng hoi gom KOH eiia Merck, propylene glycol cua Scharlau, ethanol thuc pham 95 %, nudc d l logi ion (DIW) san xuat tai Trung tam Nghien e&u Tnen khai (TTNCTK) (Khu Cdng nghe cao Thanh phd Ho Chi Minh).
Thiet bl s& dyng tai TTNCTK gom cie beaker 200 mL, 500 mL v i 1000 mL, may khuay IKA RW 20 Digital, may do UV-Vis Visco Spectrophotometer
V670, bep gia nhigt, hg dupc on nhigt cich thu^
thdng thu'dng 5& dyng nhiet k l thuy ngan chinh xae 1°C, bd ipc thuy tinh vdi mang ipc Whatman Nylon 0,2 ym, dudng kinh 47 mm va bam chan khdng.
Dau gac thuong mai cua Cdng ty GacViet' vdi thanh phan lycopene va p-carotene kiem nghigm tgi Trung tam djch vy phin ti'ch thf nghigm Thanh pho Hd Chi Minh bang phuang phip DSM v i HPLC lan lupt l i 1971,0 ppm, 6004,7 ppm. Cie gii trj do dupc eua dau gac bang UV-Vis tat TTNCTK lan lupt la 1964
± 28 ppm va 5984 ± 35 ppm.
2.2. Xac djnh dong thPi ndng dp lycopene v i p-carotene bang phd UV-Vis
Thanh phan eiia eae earotenoid ed the xae dmh dya trgn dp ha'p thy b dinh phd dac trung nio dd ciia t&ng chat b mien t& ngoai-kha kien. Lycopene trong n-hexane ed eae dinh hap thy lan lupt tgi 503 nm, 472 nm, 445 nm. Cdn p-carotene cd ba dinh b 478 nm, 452 nm v i 430 nm [2]. Do tinh cdng dupc cua dp hap thy, nlu earotenoid trich ly cd hai thinh phan chii yeu la lycopene va P-carotene thi dp hap thy d hai budc sdng 502 nm v i 450 nm cd the viet dudi dgng ham eiia ndng dp hai chat trin [3],
Aj,„^=0,320q^^+0,043C|^^^,, (1) A^^„=0,216q^+0,258C,^^,,, (2) u budc sdng 450 nm, ddng gdp cita hai thinh phan lycopene va p-earotene la gan nhu nhau, trong khi d budc sdng 502 nm ddng gdp chii yeu la eiia ly- copene. T& hg phu'ang trinh niy, gia tri nong dp ciia cac chat ed t h i suy ra dong thdi [4], [5)
q^^=3,521Ajj,j- 0,587A,j^, (3) C|^.„,=4,367A,j^- 2,947Aj^^j. (4) 2.3, Quy trinh chilt xult lycopene t& dau glc Dau gac dupc bao quan ky trong qui trinh vgn ehuyen v i dupe luu t r & d ngan mat tu Ignh d 5°Csau khi ehia thanh t&ng lypng nhd cho mdi lan thi ng- hiem. Lycopene va p-carotene la cac chuoi hydroeac- bon chua no, ed nhieu lien ket ddi nen rat d l bj oxy hoa. Qua trinh thi nghigm can phii thuc hien nhanh, khdng de dau gac, eic san pham trung gian va bpt lycopene (earotenoid) bj qua nhigt, phai sang, phPi khi hoge nhilm khuan. Dau gac thf nghigm dupc s&
dung trong vdng 30 ngay ke t& ngiy sin xuat de dam bao dp tin cgy cua kit qua (han s& dung 2 nam).
' http://www quagac.com/san-pham/dau-gac-nguyen-chat-S76.html
I 5 2 lOUKNAL OF MEDICINE AND PHAKMACV
Tqp chlYDUQc hoc - Truiyng Dm bgc Y Duac Hui - Tdp 6, so 4 - thdng 8/2016
b. Hon hqp sau xd phong hod
c. earotenoid Idng dgng sau d. Bot earotenoid trong hexane xa phong hod va nudc Hinh 1. Qui trinh tien hanh thf nghiem xa phong hoi dau gac.
Qui trinh thuy phan dau gac trong K0H/P6 dypc phat trien t& quy trinh cCia Ausich va Sanders nam 1999 J6] v i ciia Huynh eung cpng sy nam 2016 [7].
Rdt 20 g dau gac vao mpt beaker 200 mL da dupc bao giay nhdm bin ngoii d l cd the truyen nhigt dupe nhung hgn che tdi da anh sang Ipt vao (Hinh la). Beaker ditq/c dat trong be chyng each thuy d nhiet dp SOX sau dd thgm 12 g PG v i khuay deu vdi tdc dp 400 vdng/phiit trong 60 phiit. Qua trinh xa phdng hoi dupe tien hinh chim b nhigt dp 55°C bang each them t& t& 8 mL dung djch KOH 12 mol/L trong 30 phut v i ti'ep tuc khuay vdi tdc dp nhu trgn trong vdng 120 phut. 6 giai dogn niy, x i phdng cd the dupe tao ra lim dp nhdt ting nhieu, do dd can luu V de hon hpp ludn dupe khuiy deu (Hinh lb).
De giam dp nhpt v i nhiet dp eiia hdn hpp trudc khi Ipe, 50 -100 mL ethanol Ignh dupc thgm vao v i khuay deu trong vdng 15 phut sau do hg dupe bpc lgi va bao quan trong ngan m i t tu Ignh 6 nhiet dp 5-10°C trong 2-3 gid de cie chat ran lycopene va P- earotene cd the lang dan xudng diy. Luc nay mau eua dung dieh b tren it do ban, va d gan day cd the quan sat thay mpt ldp chat ran lang dpng mau do hdng (Hinh le).
Khuay nhe hon hpp rdi Ipc chin khdng bang mang Ipc 0,2 pm. Chat ran earotenoid edn Igi tren mang dupc r&a vdi hon hpp dung djch ethanoliNaCI 0,9% vdi tl Ig 1V;1V eho den khi dp PH = 7 va nudc r&a khdng edn bpt, khdng ed mau (Hinh Id), sau dd bao quan trong chai nau, say khd bang nita va xie djnh khdi lypng, thinh phin nong dp.
T& phuang phip trieh ly dy kien nhu vgy, chimg ta thay cac yeu td cd the anh hudng den hieu suit gdm nhigt dd ciia qui trinh xa phdng hoa, khdi lupng v i nong dp KOH, tdc dp khuay, dung dich rira va thdi gian lang earotenoid. Tuy nhien trong qua trinh thi nghigm khao sat sa bd, vl dp nhdt ciia dau khoang 0,050 ± 0,005 Pa.s, chung tdi chpn nong dp KOH la 12 mol/L. Luang dung d|ch KOH dupe ehpn t& 0 den 12 mL cho 20 g dau dya vao ehi sd xa phdng hoi eiia dau gac. Qui trinh x i phdng hoi xiy ra nhanh hPn khl tang nhigt dp nhung Itic nay carotenoids lgi de bj phan buy ngn trong qui trinh trich ly hg dupe dilu ehinh d 55 ± 5°C bang hg ehung each thuy. Thdi gian khao sat xa phdng hoi dau gae l i t y 60 den 150 phiit, van tdc khuay t& 200 den 500 vdng/phiit v i thdi gian earotenoid kit tinh b nhigt dp 10°C la t& 0 den 180 phut.
531
Tgp chi Y Dugc bgc - Trucmg Bqi hgc Y DUQc Hui- Tgp 6,s64- thdng 8/2016 Chung ta se khao sat lan lupt sy phy thupe eiia higu suit trfch ly theo t&ng yeu td vdi 4 khoang khao sat deu nhau. Cae kit qua dupe lay trung blnh v i x&
ly thdng k l cic bien (Analysis of Variance - ANOVA) bang MS Excel. So lieu phd hap thy UV-Vis x& ly bang phan mem OriginLabs.
3. KET QUA vA BAN llili.N
3.1. Khio sit sa bp qua trinh xa phong hoi Vdi thlit k l thi nghigm khao sat sa bp cho 20g dau, qui trinh xa phdng hda cd the diln ra gan nhu' hoan toan (san pham phan t i n tdt trong nudc va khdng cdn dau tach pha trd lgi sau khi trung hoi th&
mdt lupng nhd vdi axit HCl IM) va cd the Ipc dupe earotenoid neu lupng KOH la 8 mL 12M trong 120 phut khuay ddng hoa vdi vgn tdc 400 vdng/phiit va de lang trong 120 phut. Nh&ng thdng sd nay dupc chpn cd djnh d m&c 3 trong bang khao sit khi thay
ddi cic thdng sd cdn lgi de lam cP sd danh gii higu suat quy trinh. Qui trinh Ipc r&a earotenoid dan gian va d l dang han so vdi phuang phip cua Ausich v i Sanders 1999 [6] v i ciia Huynh [7] vl hgn che sir dyng thgm nudc v i khdng trung hoa bang axit HCl lam tang dp nhdt eua dung djch va se tgo thanh Idp ming sip ngin can qua trinh Ipe khi s& dyng bom chan khdng.
Hinh 2 the hign phd UV-Vis eiia 3 mlu bpt ear- otenoid khac nhau trong dd M I U C l i san pham thu dupc bdi quy trinh xa phdng hoi d tren. Miu A dupe Ipc r&a va t i i k i t tinh trong hexane n§n cd ndng dp lycopene eao va ed the quan sat ro 3 dinh die tru'ng ciia lycopene nhu kit qua eiia tac gia khac [8]. d Miu B (th& nghiem thdi gian loe vi say lau trong ban khdng khi) dinh d 502 nm khdng cdn (Bang 1) va dinh dgc trung d 450 nm trd nen trpi han.
0 . 9 - 0 , 8 - 0,7- 0,6- 0.5- 0,4- 0,3- 0,2- 0,1 -
/ '
K : : : - ^ ' '
• ' • 1
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1 l \ -
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p
V 'i
V V
1
- - -Mau A - • • M a u B
Mau C
1
Bu'd'c sdng (nm)
Hinh 2. Pho UV-Vis trong n-hexane ciia cic mlu bpt earotenoid thu dupe vdi ti lg lycopene;P-carotene khac nhau (xem thgm Bang 2).
Phuang phap xic d[nh thanh phan cua cic earotenoid phd bien nhat l i phan ti'eh phd t& ng- ogi kha kiln UV-Vis va sle ky long higu nang cao (HPLC-high performance liquid chromatography), Phuang phip HPLC cd the phan tach va xic djnh thinh phan eae earotenoid mdt each chinh xae.
Tuy nhign eung chfnh vgy phyang phip nay khi tdn kem, cln nhieu hoa chat khi dpc hgi, nhieu thdi gian va k? thuit ph&c tgp. Vdi chi phi thdng thudng va quy trinh xac djnh dan gian han nhieu,
I 5 4 JOURNAL Of MEDICINE AND PHARMACY
phu'ang phap UV-Vis da dupc s& dung rpng rai trong eae nghien e&u eho phep thu thip cac thdng sd nhanh v i kha chfnh xic. Thgt vgy, Ab- dul-Hammed s& dyng cic he phuang trinh trgn va khao sit Igi dp hap thy ciia lycopene b dinh 472 nm cho k i t qua phii hpp den 98% vdi gii tri eua cic nghien e&u ly thuyet [5]. Trong bai bio nay, chting tdi gidi hgn xac djnh thanh phin hai earote- noid chii yeu ciia sin pham d l danh gia higu suit ciia qui trinh trich ly dya tren phd UV-Vis.
Tgp cbi Y DUVC hgc - Tru&ng Dgi hgc Y DUQC Hue -Tgp 6, so 4- thdng 8/2016
Bing 1. Dinh phd hap thy v i thinh phan lycopene, P-carotene trong cie miu b Hinh 2.
Mlu VI tri 1 Vi trf 2 Vi tri 3 Vi tri 4 A 450 A 502 C lyc C Bear " , "^ .-f'j^^ ' • - - ~ ' ~ '^ •<-C_lvc /C_lyc A
B C
422*
424*
423*
445 450 449
471 475 472
502
502*
0,487 0,853 0,722
0,566 0,254 0,340
1,706 0,392 0,773
0,457 2,979 2,151
2,164 3,371 2,924
0,268 7,593 2,782 Dan VI do ndng dd l i mg/L.
Bing 2. Higu suit ciia quy trinh trich ly
Thinh phan Khdi lu'png ban dau Khdi lypng thu dypc Hieu suit Lycopene
p-carotene Tong cdng
0,0394 ±0,0022
0,1201 ±0,0012
0,1595 ± 0,0026
0,0242 4 0,0023
0,0796 ± 0,0060
0,1038 ± 0,0083
61,42 ± 3,59
66,27 ± 4,63
65,07 ± 4,22
Dan vj do khdi lu'png g, hieu suat %. Ket qua lay trung blnh 3 lan thf nghigm.
Bang 1 t h i hign dinh phd hap thy va thinh phan lycopene, p-earotene trong cac mau b Hinh 2. T&
ket qua eCia M i u C, higu suat ciia quy trinh trieh ly cic earotenoid trong 20 g dau d\JtQc the hi6n b Bang 2. Higu suit trieh ly lycopene thap han cd t h i la do hogt ti'nh oxy hoi cao cua nd so vdi p-carotene.
Tdng lupng chat ran thu dupc 0,1165 ± 0,0153 g.
Higu suit trich ly chat ran la 73,11 ± 0,02%. Dp tinh khiet dgt 89,02 ± 6,25%, dupe ti'nh bing tl le earo- tenoid tdng epng trong chat ran Ipc dupc. Hieu suat Chung ciia qua trinh la 65,07 ± 3,84%. Ti Ig lycopene va P-carotene it thay ddi so vdi liie dau eh&ng td cae dieu kign thi nghiem dtren cd kha nang bao vg tdt lycopene cung nhu eic earotenoid khic.
3.2. Khio sat higu suit it cic thdng sd khic nhau
BSng 3. Khao sat higu suit quy trinh theo thdng sd thi nghigm Thong so
Luong KOH 12 M (mL) Thc'r gian xa phong
(phut) Toe dp quay (Vong/phut) Thcri gian lang
(phut)
^oa 1 0 0 ± 0 "
60 38,03 ± 1,06'
200 41,85 ± 4,20^
0 32,43 ± 6,0r
2 4 29,15 ± 4,46"
90 59,27 ± 4,27''
300 54,79 ± 2,90''
60 51,38 ± 3,69^
3 8 65,07 ± 3,84'
120 65,07 ± 3,84"
400 65,07 ± 3,84'
120 65,07 ±3,84'
4 12 65,21 ± 2,96'
150 57,43 ± 5,48'
500 65,58 ± 1,92"
ISO 66,19 ± 2,17' Dan V! higu suat %. Ket qua lly trung blnh 3 lln
thi nghigm. Cac gia tri tren eiing mpt hang ed cic chi sd khae nhau (a, b, c) cd khac bigt thdng ke vdi m&c tin cgy 0,05.
Bang 3 tdng hpp ket qua khio sat hi|u suat eCia qui trinh trich ly theo cae thdng sd thf nghigm. Kit qua cho thay lu'png KOH anh hudng ehinh d i n higu suit qua trinh. Hieu suit ting gan ti Ig thugn vdi lypng KOH nhung t& 8 m l trd lgn, higu suat thu dupc tang rat it vi l&c nay qua trinh x i phdng h o i
diln ra gan nhu hoin toan. Luang KOH ting va du sau xa phong hoi qua nhieu la khdng can thilt, v&a ton kem hoi ehat, v&a khd r&a va Ipc.
Higu suat trich ly cijng tang khi tang tdc dp khuay t& 200 d i n 400 vong/phOt. Tuy nhien gia trj niy khdng the tang nhieu han do kha nang cua may va do dp nhdt cua hg.
ThPi gian x i phdng hoi ting se lam ting ham lupng earotenoid thu dupc va giiip phan &ng ed the xay ra hoan toan. Tuy nhien nlu xa phdng hoi qui
AND PHAHMACY 551
Tgp cbi Y DUQC hgc - TruVng Dgi hgc YDuQc Hui- Tqp 6, so 4 - thdng 8/2016
lau trong KOH cd t h i lam giam ding k l lupng higu suit do cac earotenoid giai phdng ra b| tilp xdc vdi khi oxy v i nhigt trong thdi gian dai. Tdc dp khay v i thdi gian x i phdng hoa la hai y l u td cd anh hudng qua Igi din kit qua. Thdng thudng tdc dp khuay cang nhanh thi phan &ng xay ra cang nhanh do eic chat tham gia phan &ng tilp xtic nhau d l han. Kit qua thf nghigm phii hpp vPi dy doan ly thuyet nay.
Sy phy thupe eua higu suat va thdi gian lang eiia dung dich ed the do sy kit ty cua cic tinh the diln ra de dang han khi lam lanh dpt ngpt. Tuy nhien khi tangthPi gian lam Ignh hoac nhigt dp ciia hdn dich thap dudi 10°C cd t h i lim he ddng Igi va khd Ipe.
3.3. Phit trien k i t qui trich ly bpt lycopene Th& nghigm trich ly vdi cic thdng sd mang Ial higu suat cao nhat, t&c la diing 12 ml KOH 12M trong 120 phut, khuay vdi vgn tdc 500 vdng/phiit v i de ket tinh trong 180 phiit, kit qui higu suit thu dupe la 66,83 ± 4,52%. Higu suat tang khdng nhieu trong khi KOH nhieu han va thdi gian thyc hign dai ban nen ed the lam giam tinh kinh t l ciia quy trinh.
De tim hieu tinh dn djnh ciia phuang phap va kha nang nang cd san xuat pilot, thf nghigm dupe lap lai vdi lupng dau gae la 50 g va 500 g, s& dung bd loe kieh thude ldn han. Hieu suat thu dupe lan lupt la 66,25 ± 4,08% va 68,05 ± 2,59%. Ket qua cho thay hieu suit ciia quy trinh kha on djnh va mat khic cd the nang cao ban khoing 2 den 3% vi kha nang thu
hdi earotenoid t& giay ipe eao han khi tang quy mo thf nghigm. Vdi uu the trong phan bd trai glc - "trai eay t& trdn trdi", cung nhu' vai trd quan trgng ciia lycopene vdi ham lupng ldn trong gac, vigc dau tu nghign cyu san xuat va dng dyng cae san pham t&
gae tgi Vigt Nam h&a hen tiem nang trong tuang lai.
4. K^TLUANVAKIENNGH)
earotenoid ndi ehung va lycopene ndi rigng rit dupc quan t i m do cd vai trd dang ke ddi vdi sue khoe eon ngudi va cic yng dyng trong cong nghigp mau thyc pham, thyc pham ch&c nang v i my pham.
Lycopene tan t y nhien trong diu gae nen hien nay cic sin pham tren thi trudng ehii yeu la dau gac Tuy nhien cic enzyme ton tai trong dau gac cd the phi buy lycopene. De tang pham vl &ng dung cijng nhtr sy da dgng eiia san pham, chiing tdi nghiin c&u cich trich ly lycopene va dy kiln phit trien dang nano phan tan trong nu'dc.
Quy trinh trich ly lycopene va p-earotene t& dau gac bang phUPng phip x i phdng hoi chl diing KOH va PG la mpt phuang phip khi thi, an toan va it tdn kem vdi higu suat dgt 65,07 ± 3,84%. San pham eb the s& dyng ngay trong thye pham va my pham. Quy trinh dn djnh v i cd kha nang phit triln b quy m l pilot. LOe nay, eic thiet bj v i qui trinh Ipc cd t h i dieu ehinh phii hpp de dgt higu qua cao nhusfrdyng Ipc li t i m v i say phun Ignh san pham trong ddng khi nita.
TAI LI|U THAM KHAO
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