KHOA HOC CbNG NGHfi
IXIGHIEIM CliLI IJIVG DLJIMG ElVZYIVIE O E TACH LOP IMHOT CUA CA
P H £T H d C TROIMG CdlMG
I M G H £CHE BIEIM UOT
Nguydn Vdn Thudng^, Phan Thanh BinhS Phan Thi Loan^
TOMTAT
Nghidn ciiu dupe thpc hidn tir nam 2009-2011 tai Budn Ma Thudt Nam chd pham enzym tiiuc phlm, gdm:
Lumipect-CP, Peelzym®, Rohapect® lOL Rohament® pulpex va Pectinex® Ultra SP-Ld\iac thii nghidm vd hidu luc phan hiiy dich chidt lop nhdt dinh trdn hat c i phd flidc sau khi xat bd vo qua. Da ket luan Rohapect® lOL la chd pham phii hpp nhdt dd six dung tdch lap nhdt ciia qua ca phd vdi. Cung da xac dinh dupe lidu lupng Rohapect® lOL thich hpp nhdt dd tach lop nhdt ca phd vdi trong thdi gian 2 gid la 150 ppm.
vdi ti Id pha trdn ca phd/nu6c la 10/1 theo khdi lupng.
Tirkhda: Ciph€ vdi, chd bidn udt, tich lop nhdt, enzim, chung lo^, liSu lugng.
LBATVANDE
Chd bidn cd phd nhdn cd thd thtrc hidn tiieo cdng nghd khd hoac cdng nghd udrt. Mdt cdng doan quan trpng trong chd bidn theo cdng nghd irdt la tdch Idp nhdrt ndm trdn lop vd thdc cua hat sau cdng doan xdt bd vd qua. Thanh p h i n chinh ciia lop nhat Id cac hpp chit cao phdn tir n h u pectin, duong vd xenluloza [11.(3]. Pectin vd xenluloza khdng tan trong nirdc vd ndu khdng dirpc loai bd thi tao ra nhidu nguy c a lam giam c h i t Itrpng cd phd vd gdy khd khan cho cdng vide phai s l y sau nay.
Hai phtrang phdp tdch nhdt phd bidn hidn nay Id
~ Idn men tu nhidn vd ddnh nhdt c a hpc. Mdi phuang phdp ddu cd han chd n h l t dinh:
Ldn men tu nhidn phai keo ddi 1648 gidr. vi vdy de tranh Cm tdc san p h l m chd bidn khdng kjp d n phai xdy dung nhidu bd ldn men tdn kem. Qud trinh ldn men chiu nhidu tdc ddng khdng kidm sodt dupe tir bdn ngodi. n h l t la ydu td nhi^t dp, do dd kdt qua khdng dn d{nh. C h i t lupng d phd cd nguy c a bj gidm do nhidu hat bj ldn men thdi dan ddn d c miii vj khd chiu trong nudrc pha. Ldn men tu nhidn lam tang muc dp d nhidm nude vd khdng khi.
Phuong phdp ddnh nhdt c a hpc khdc phiic dupe mdt vai b i t lpi cua p h u a n g phdp ldn men tu nhidn, nhtmg nd lai cd cdc b i t lpi khdc: thidt b | cd gid khd cao; tidu tdn nhidu didn; nguy c a ldm nhidu hat bj tide vd thdc vd cd phd nhdn khdng cdn Idp bao vd ndn khd bdo quan. Do d c mdy ddnh nhdt khdng tdch dupe nhdt ndm tai khe hat ndn ndu mudn loai bd hdt nhdt phai ldn men bd sung vd khi dd qud tiinh chd bidn trdr ndn phtic tap horn.
Dd trdnh dupe nhimg han chd trong d hai phuang phdp ldn men tti nhidn vd ddnh nhdt ca hpc, d c nhd khoa hpc dd nghi sii dting enzim dd phdn hiiy lap n h d t Mdt sd hang san x u l t enzim trdn tiid gidi da gidi thidu cdc chd p h l m enzim phan huy lop nhdt ciia cd phd.
Trudc khi dd nghi ting dung enzim dd phdn buy Idp nhdt cd phd trdn quy md ldn or Vidt Nam, vide dau tidn Id phai xdc dinh dupe loai chd p h l m enzim phii hpp vd cdc didu kidn thich hpp dd chd p h l m phdn huy lop nhdt dat ket qua tdt n h l t Dudi ddy la kdt qua tuydn chpn chd p h l m enzim tiiich hpp n h l t dd phdn hiiy lop nhdt cua qua cd phd vdi (Robusta) qua vide ddnh gid hidu luc phdn hiiy djch chidt lop nhdt cua chting vd kdt qua xdc djnh Udu lupng enzim tuydn chpn phu hpp cho xir ly Idp nhdt trong thdi gian 2 gidr. An djnh thdi gian xir ly 2 gidr dd phii hpp vdi thuc td san x u l t Sau khi xir ly enzim, cd phd cd thd dem phai s l y ngay trong ngdy chd bidn.
L NGUY^ LtU VA PHUOniG PHAP 1. Tuydn chpn chd p h l m enzim
- Qua chin cd phd vdi dirpc tdch vd bdng mdy xdt trdng tiidng thudng dd lly ra 30 kg cd phd thdc. Cd phd thdc dupe chia lam 6 phin, mdi phin 5 kg, dung trong cdc chdu i-nox;
- Cho thdm vdo mdi chdu 2,5 Ut nude (ti Id 2 cd p h d / 1 nude). Dung mdy ddnh tning hai cdnh khuly dd k h u l y dao ky cd phd d mdi chdu trong thdi gian 10 phiit dd chidt x u l t Idp n h d t
Tdch ridng hat cd phd ra; trdn chung djch chidt ciia 6 chdu dd cd m l u chung, sau dd lai chia ddu cho 6 chdu dd cd m l u tiii nghidm xii ly enzim.
'Vi§n KHKT Ndng LSm nghidp Tdy Nguydn
^Trudng Cao dang nghe Thanh nien dan tpc Tay Nguyen
N 6 N G
NGHlfP VA PHAT TRIEN
N 6 N G T H 6 NKYI THANG 8/2011
49- ChuIn bi 5 chd pham enzim (ndu trong bang 1).
Cac chd phlm nay chiia enzim pectinaza hodc xenluloza, hoac hdn hpp enzim tiiudc hai nhdm [2].
Moi chd phlm xu ly dich nhdt a mdt chdu ridng bidt voi Udu lupng 15 ppm tinh theo khdi lupng d phd thdc udt Dich chidt trong chdu khdng xir ly enzim la ddi chiing.
Bang 1. Cdc chd phlm enzim thtrc phlm sii dting trong nghidn ciiu Chdphlm
Lumipect-CP
Thdnh phin enzun Polygalacturonase (vidt tdt PG, thudc nhdm pectinaz^
Ngudn gdc San phlm ciia Lumis Biotech P.Ltii„ An Dp
Tinh chit Long, mdu ndu, tan trong nude. Hoat lire toi tiiidu: 720 LU/ml.Ti trpng: 1,25 g/ml.
Peelzym®
Enzim nhdm xenluloza, chu ydu p-glucanazs, ngodi ra cdn cd xylanazav^ hemicellulaza nhung hoat Itic thIp
San pham ciia Novo Nordisk Ferment Ltd.
Dan Mach. San xult bdng gidng nim AspergUlus aculeatus.
Long, mdu ndu. miii ldn men nhe, tan trong nude. Hoat Itrc tdi thieu:
lOOFBG/g.Titi-png:
1,23 g/ml
Rohapect® lOL
Enzim nhdm pectinaza, gdm pectinesteraza (PE), PG vd pectinlyaza (PL)
San phlm ciia AB Enzim, Phin Lan. San xult tii gidng nim
AspergiUus niger.
Long, mdu ndu, miii thorn, tan trong nuoc.
Hoat Itic tdi tiiidu: 260 PA Titi-png: 1,18 g/ml.
Rohament®) pulpex
Enzim nhdm pectinaza gdm PG, PE. PL; ngoai ra cdn cd mpt sd enzyme nhdm xenluloza nhtmg hoat tinh thap
San phlm ciia AB Enzim, Phin Lan. San xult tii gidng nim
AspergiUus niger.
Bdt thd, mdu ndu nhat, tan trong nude. Hoat lire tdi tiiidu: 600 PA.
Pectinex® Ulti-aSP-L
PG, ngoai ra cdn cd
hemicelUulaza nhung hoat tinh
rIt thIp
San pham cua Novo Nordisk Ferment Ltd, Dan Mach. San xult tir gidng nim
AspergiUus aculeatus.
Long, mdu ndu, miii ldn men nhe, tan trong nude. Hoat lire: 9500 PGU/ml.Titi-png:l,16 g/ml
Su dtmg hai loai vi nhdt kd VT-03F va VT- 04F cua Nhdt Ban dd do dp nhdt cua djch chidt ngay tai thdi diem tnrdc khi xir ly enzim vd sau mdi 6 phtit Dan vj dp nhdt tinh bdng mPa.s (MilUpascal-gidy).
Mire dp giam dp nhdt nhanh hay cham thd hidn hidu luc ciia enzim.
2. Xdc djnh Udu Itrpng chd phlm enzim thich hpp Qua chin cd phd vdi dirpc tdch vd bdng may xdt trdng dd lly ra 105 kg cd phd thdc. Chia ddu cd phd thdc vdo 35 chau (mdi chdu 3 kg) vd phdn lam 7 nhdm (mdi nhdm 5 chau). trong dd mdt nhdm khdng xii ly enzim, vd 6 nhdm dupe xu ly enzim Rohapect®10L tuong ting vdi 6 Udu lirpng (tinh bdng ppm, w/w) (bang 2). Nam chdu or mdi nhdm dupe bd sung nirdc theo d c ti Id ca phd/nuoc (w/w) lln lupt la 10/5, 10/4, 10/3. 10/2 va 10/1. Dao ti-pn cd phd vdi enzim ky ludng va ti trong 2 gidr. Rura cd phd 3 lln bang 15 Ut nudrc sach/chdu vd dem phoi kho. Kidm tra vd danh gid mtic dp sach nhdt theo phirong phdp chuydn gia. Xdt khd va tinh ti Id % khdi lupng cd phd
nhdn khd (Im dO 12,5%) so vdi d phd flidc udt tiirdc khi xii ly enzim. Ldp lai thi nghidm 3 lln.
Bang 2. Cdc cdng thtic thi nghidm xii 1^
enzun Rohapecl®10L Cdng
thiic Ddi chiing
I II II IV VI
Lidu lupng enzim Oppm 100 ppm 150 ppm 200 ppm 250 ppm 300 ppm 350 ppm
Ti Id ca phd/nuoc 10/5
10/5 10/5 10/5 10/5 10/5 10/5
10/4 10/4 10/4 10/4 10/4 10/4 10/4
10/3 10/3 10/3 10/3 10/3 10/3 10/3
10/2 10/2 10/2 10/2 10/2 10/2 10/2
10/1 10/1 10/1 10/1 10/1 10/1 10/1 Sd Udu xii ly bdng phin mdm MSTATC d a D?i hpc Michigan. Ddnh gid chit lupng miii vj d phd tdch theo phuang phdp cho didm cua Hidp hpi Cd phd Ddc san Hoa k^ (SCAA) vdi 6 chi tidu chinh vA tdng sd didm miii vj. Cdc chi tidu: Miii bdt (Fragrance), Miii nude (Aroma), thd chit (Body), dO axit (Acidity) vd miu-vj (Flavor) tinh theo thang didm
50
NONG NGHIEP VA PHAT TRIEN
N 6 N G T H 6 NKY 1 - THANG 8/2011
KHOA HOC CbNG NGHfi 10; chi tidu miic ua tiiich cua chuydn gia tiiir ndm
(OveraU) tiieo thang didm 20.
•.KETQUAVATHAOLUAN
1. Hidu qua tdch nhdt cd phd ciia cdc enzim thi nghidm
cd tdc dung ldm giam nhanh dd nhdt cua djch tridtidp nhdt cua hat cd phd.
Dd nhdt cua djch chidt ldp nhdt trudc khi xii ly enzim Id 270 mPa.s; sau khi xu ly, Peelzym® ldm giam dp nhdt nhanh nhlt (sau 18 phiit dp nhdt djch chidt cdn 20 mPa.s) vd tidp tiieo Id Rohapeet®10L
(sau 24 phiit dp nhdt djch chidt cdn 25 mPa.s).
Kdt qua or bang 3 cho thly d nam loai enzim ddu
Bdng 3. DQ nhdt (dvt niPa.s) ciia djch chidt I6p nhdt cd phd sau khi xu ly enzim Thdi gian
xir ly (phiit)
0 6 12 18 24 30 36 42 48 54 60 66 72 78 84
Cdc cdng thuc xir ly enzun Lumipect -
C P 270 230 185 155 110 95 80 70 65 60 55 50 50 45 28
Peelzym®
270 60 50 20 17 15 13 10 10 10 10 8 8 7 7
Rohapect®
lOL 270
70 60 35 25 17 16 15 14 13 11 10 12 10 10
Rohament®
Pulpex 270 155 118 60 40 17 9 6 5 5 5 5 4 4 4
Pectinex® Ultra SP-L
270 140 100 65 29 26 23 20 19 17 17 16 16 16 16
Ddi chiing
270 270 270 265 265 260 260 260 250 250 250 250 250 250 250 Rohament® Pulpex or giai doan dIu khdng ldm
giam dp nhdt nhanh nhu Peelzym® vd Rohapect®10L, nhung or giai doan sau lam dd nhdt giam bidt dd nhlfc sau 48 phtit, dp nhdt djch chidt chi cdn 5 mPa.s, chi bdng 1/2 so vdi djch chidt xii ly Peelzym® vd bdng 1/3 so vdi djch chidt xir 1^
Rohapect®10L. Luu y rdng dd nhdt ciia nude Oly hi gidng khoan) sii dung trong thi nghidm ndy la 0,7 mPa.s.
Hinh 1 bidu didn qud trinh gidm dd nhdt cua djch chidt bdng sd tuong ddi (%) trong dd dp nhdt cua dich chidt trudc khi xu ly enzim la 270 mPa.s tuong duong 100%. Vdi ddi chung sau 30 phut dp nhdt vin cdn 94,7%; vdi d c cdng tiiuc xir ly enzim, dp nhdt chi cdn 5-33%. Sau 84 phiit dp nhdt d a ddi chung vin cdn 87,7%, ti-ong khi dd nhdt cua cdc cdng tinic xu ly enzim chi cdn dudi 10%.
Trong 5 chd phlm, Lumipect CP cd hidu luc tiilp (sau 30 phtit dd nhat van cdn 30%). Cdn lai 4 enzim khdc cd hidu lt,rc tdt sau 30 phiit ldm dd nhdt dich chidt gidm cdn 5-10%; tiiy vdy ti-ong 4 loai ndy.
Pectinex® Ultra SP-L cho hidu luc kdm hon ba loai cdn lai.
• - UnipEd- CP
•—Pcel^TD*
RohaoicntV Pulpex K-PeclineslliUIIriSP-L
»—Doi chung Dich nh6t
^— Doi chui^ Nude
0 6 12 11 24 30 36 42 48 S4 60 66 72 71 84 Thbi gian xu ly (phut)
Hinh 1. Mtic dO giam dd nhdt d a djch chidt Idp nhdt d phd sau khi xii ly enzim
Chd phlm Peelzym® cd hidu luc rIt cao, ldm giam dd nhdt ciia djch tiidt lop nhdt nhanh nhlt: chi sau 18 phut xir ly, dp nhdt ciia djch tridt ldp nhdt chi cdn 7%. Rohapect®10L cho cimg cd hidu luc rIt cao;
sau 24 phiit xii ly, dp nhdt ciia djch chidt cdn dudi 10%. Tuy vdy khi xir ly bang Peelzym® tiii d phd rIt khd riia sach vd vd tiidc xin mdu sau khi khd.
N 6 N G
NGHIEP VA PHAT TRI^N
N C N G T H 6 N- KY 1 - THANG 8/2011
51Rohament® Pulpex cung cho hidu Itic cao. Theo cdng bd, hoat luc pectinaza d a chd phlm la 600 PA, cao han 2 lln so voi Rohapect®lOL (260 PA); tuy vdy ket qua nghidn cthi lai cho thly hidu Itic phdn huy djch nhdt cua Rohapect®lOL cao hon. Do bdo chd d dang bpt ndn Rohament® Pulpex khd hda tan vao djch nhdt han, vi vay phdt huy tac dung cham han.
Vdi uu tidn lua chpn chd pham lam giam dp nhdt djch tridt xudng dudi 10% nhanh n h l t ndn Rohapect®10L dupe chpn cho d c nghidn cihi tidp theo.
2. Hidu qua tdch Idp nhdt d phd d a Rohapect®
lOL dr d c Udu lupng khdc nhau
Bang 4 cho thly or cac ti Id cd phd/nuoc khac nhau thi mtic dp sach nhdt ciia d phd thdc ddu tang din theo Udu luong enzim.
TI Id d phd/nuoc cang cao thi mtic dp sach nhdt cdng cao. Khi ti Id d phd/nuoc cang cao, tiic lupng nude dimg cdng i t thi dung djch enzim cdng dSm dac, do vay hoat lire enzim se cao han vd tac dung phdn hiiy ldp nhdt se nhanh han so vdi ti Id ca phd/nuoc thIp (dung djch enzim loang hon). Voi cdng thuc ddi chting (khdng xir ly enzim) a mpi ti 16 cd phd/nuoc, sau 2 gidr dp nhdt mdi sach 10%.
Bang 4. Mtic dd sach nhdt (%) d a cd phd dr cdc Udu lupng Roh^ject® lOL vd ti Id d phd/nudc Lidu lupng enzim
10/5 10/4
TI Id d phd/nudc
10/3 10/2 10/1 Trung binh
Oppm 10 10 10 10 10 10
100 ppm 65 65 65 75 85 71
150 ppm 70 70 70 80 95 77
200 ppm 75 80 80 85 95 83
250 ppm 80 80 80 90 95 85
300 ppm 85 85 85 95 100 90
350 ppm 90 95 100 93
enzim tiilp nhlt dap ting ydu clu nay Id 150 ppm theo ti Id cd phd/nuoc 10/1.
3. Anh hudng d a Udu lupng Roh^)ect® lOL tdi khdi lupng san phlm
Ti Id d phd nhdn thu dupe giam din theo su tdng ciia Udu lupng enzim Rohapect® lOL ttr 10O-350 ppm (bang 5). Khi xir ly enzim vdi Udu lupng cdng cao, ldp nhdt cdng bj phdn htiy nh<uih vd kha ndng enzim lam mdng vd thdc, giiip mdt sd chit tan tir Hinh 2. Mdu sac d phd tiidc khd dupe xir ly trong hat tiiodt ra khdi vd ttidc cdng dd hon, vi vHy
Rohapect vdi ti 1$ 10 d p h d / 1 nude khdi lupng nhdn giam nhidu hon. 6 Udu lupng 150 Cd phd tilde cd miic dp sach nhdt 95% trdr ldn Ppm, trung binh ti 1^ cd phd nhdn thu hdi tii^ hon dirpc coi la sach, vi vdy cdng thiic xti ly Udu lupng ddi chiing 0,5%, day la mtic chap nhdn dupe.
Bang 5. TI Id d phd nhdn khd b d c Udu lupng enzim vd ti Id d phd/nudc Udu lupng enzim
10/5
% (w/w) cd phd nhdn thii hdi theo ti Id cd phd/nudc
10/4 10/3 10/2 10/1 Trung binh (*)
Oppm 33,3 ±0,2 33,7 ±0,1 34,0 ±0,2 35,0 ±0.2 35,4 ±0.3 34.3 a 100 ppm 33,0 ± 0,2 33,8 ±0,2 34,1 ± 0,2 35,0 ±0,3 36,2 ±0,2 34,4 a 150 ppm 32,4 ±0,3 33,5 ±0,1 33,4 ±0,2 34,3 ± 0,1 35,3 ±0,2 33,8 A 200 ppm 31,6 ±0,3 33,3 ±0,2 32,2 ±0,4 32,3 ±0.5 35,3 ±0,3 33,0 c 250 ppm 31,4 ±0,2 32,4 ±0,3 31,3 ±0,3 32,2 ±0,2 35,1 ±0,1 32,5 rf 300 ppm 31,3 ±0,3 31,6 ±0,4 31,4 ±0.3 31.8 ±0,1 34,5 ±0,1 32,1 rf 350 ppm 30,1 ± 0,1 31,3 ±0,2 30,9 ±0,3 31,4 ±0.3 34.4 ±0.2 31,6 f (*) Cic sdUdu a cdt ndu tiieo sau bing cic chu cii gidng nhau thi khdng khic nhau vS mit tiidng ki (p>0,05) theo tiic nghidm da doan Duncan.
52 NONG NGHIEP VA PHAT TRIEN NONG THON KY 1 - THANG 8/2011
KHOA HOC CbNG NGHfi
Ti Id d phd nhdn tiiu hdi or cdng tinic xii ly 100 Chat lupng cd phd tdch ctia cd phd dupe xu ly lop ppm sai khdc khdng cd y nghia tiidng kd so voi ddi ^^^ bdng enzim h l u nhu khdng khdc bidt rd rdt so chung, cdn lai dr cdc cdng tiiiic khdc ddu tiilp ban ddi ^di ddi chirng khdng x u ly enzim, n h l t la didm sd vd chung. Khdi lupng cd phd nhdn tiiu dupe tdng d i n ™"i-vi- Khi tang d i n Udu lupng enzim tiii miii tiiom theo si; tang ciia ti Id cd p h d / n u d c vd sir khdc bidt vd ^d tiid c h i t d a cd phd. tdch gidm di chiit i t ma ti Id cd phd nhdn cd y nghia tiidng kd tiieo t r i e "guy^n nhdn cd tiid Id do mdt lupng c h i t hda tan nghidm da doan Duncan. 6 ti Id 10 cd p h d / 1 nude " ^ I t djnh da di ra khdi nhdn trong giai doan tdch khdi lupng cd phd nhdn cao n h l t so vdi cdc ti Id khdc.
Kdt qua xu ly tiidng kd cho tiily khdng cd sir tuong tdc giua hai ydu td Udu lupng enzim vd ti Id cd phd/nudc tdi ti Id cd phd nhdn tiiu dupe ^>0,05).
Xdt khia canh tiet kidm chi phi san x u l t dd lam tang hidu qua cua chd bidn tiii a ti Id 10 cd p h d / 1 nuoc voi Udu lupng enzun 150 ppm da cd kdt qua t d t lupng enzim sir dung tiilp horn bdn cdng tiitic khdc md cd phd vin sach nhdt vd ti Id cd phd nhdn thu dupe 35,3%, thap hon ddi chiing chi 0,1%.
nhdt vd ldm cho dd dam cua tdch cd phd (Udn quan chat che ddn tiid chit) giam di it nhidu.
W.KFTUIAN
Trong cdc chd p h l m chtia enzim pectinaza thi nghidm, Rohapect®10L cd hidu lire phdn hiiy djch chidt ldp nhdt cua qua cd phd vdi cao n h l t Voi lidu lupng 15 ppm, chi sau 24 phiit xir ly da lam giam trdn 90% dp nhdt cua djch chidt ldp nhdt.
Lidu lupng Rohapect®10L tiiich hpp cho xii ly ldp nhdt cd phd la 150 ppm vdi ti 1^ 10 cd phd/1 nude, 3. Anh huong ciia Udu lupng Rohapect® lOL ddn lam sach 95% lupng nhdt vd hao hut khdi lupng ca
Xii ly ldp nhdt cua hat cd phd bdng chd pham Rohapecl®10L khdng ldm dnh hudmg x l u ddn miii vj cd phd tdch.
TAI U ^ THAM KHAO
1. Carlos H. J. Brando (2004). Harvesting and Green Coffee Processing, Coffee Growing, Processing, Sustainable Production. Wiley-VCH Verlag Gmbh & Co. KgaA, Germany, 605-714.
2. Tdi Udu ky tiiudt (2009) cua cdc hang AB Enzyme, Novozyme vd Greentech.
3. Wafa Masoud and Lene Jespersen (2006).
Pectme degrading enzymes in yeast involved infermentation of Coffee arabica in East Affica.
International journal of Food Microbiology 110 (2006), Elservier, 291-296.
chit lupng c^
Bin);
Udu lupng enzim Oppm 150 ppm 200 ppm 300 ppm 350 ppm
Mui bpt
8 8 8 8 7
I phd tdch
' 6. Kdt qud thu ndm cdc m i u cd o Miii
nude 8 8 8 7 7
Thi chdt
8.5 8.5 8 8 8
DO acid 4 4 4 4 4
Miii- vj
7 7 7 7 7
Mtic ua thich
17 16 16 17 17
hd Tdng didm 52.5 51.5 51 51 50 Lly mdt sd mau san p h l m tir thi nghidm Udu lupng Rohapect vdi ti Id 10 cd p h d / 1 nude vd mau d phd nhdn chd bidn udt (khdng xti ly enzim. ldn men tu nhidn) dd tiiir ndm tdch.
POSSIBLE APPUCATIONS O F ENZYMES AT MUCILAGE - LAYER REMOVING STAGE D4 COFFEE WET PROCESSING TECHNOLOGY
Nguyen Van Thuong, Phan Thi Loan, Phan Thanh Binh Summary
The research was carried out during coffee harvest crops 2009/10 and 2010/11 in Buon Ma Thuot.
Vietnam. Five enzyme preparations, covering Lumipect-CP, Peelzym®, Rohapect® lOL Rohament®pulpex and Pectinex® Ultra SP-L were assessed its ability for removing mucilage layer on coffee parchment from pulped coffee cherries. The regjilt shown that enzyme preparation Rohapect® lOLis the best; and the most suitable usage dose of this enzyme preparation is 150 ppm for treatment of parchment coffee in a duration of two hours.
Key words: Robusta coffee, wet processing, mucilage removing, enzyme and usage dose.
Ngudi phdn bidn: TS. T r I n Thj Mai
N 6 N G
