جاﺮﺨﺘﺳا،تﺎﺒﯿﮐﺮﺗ ﯽﯾﺎﺳﺎﻨﺷ ﯽﯾﺎﯿﻤﯿﺷ و ﺖﯿﻟﺎﻌﻓ ﯽﺑﺎﯾزراﺪﺿ ﯽﺑوﺮﮑﯿﻣ ﺲﻧﺎﺳا ﺶﻔﻨﺑ ﻮﺑزﺎﻧ ﺮﺑيﺮﺘﮐﺎﺑيرﺎﻤﯿﺑ يﺎﻫﯽﯾاﺬﻏ ءﺎﺸﻨﻣ ﺎﺑ از ﺎﺑ نآ ﻪﺴﯾﺎﻘﻣ و ﯽﺘﻧآﺗﻮﯿﺑﯿﮏﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ و ﻦﯿﺴﯾﺎﻣﻮﮑﻧو يﺎﻫ ﯽﻌﯿﻤﺳ ﻪﻧﺎﺴﻓا

جاﺮﺨﺘﺳا

، تﺎﺒﯿﮐﺮﺗ ﯽﯾﺎﺳﺎﻨﺷ ﯽﯾﺎﯿﻤﯿﺷ

و ﺖﯿﻟﺎﻌﻓ ﯽﺑﺎﯾزرا ﺪﺿ

ﯽﺑوﺮﮑﯿﻣ ﺲﻧﺎﺳا

ﺶﻔﻨﺑ ﻮﺑزﺎﻧ ﺮﺑ

يﺮﺘﮐﺎﺑ يرﺎﻤﯿﺑ يﺎﻫ

ﯽﯾاﺬﻏ ءﺎﺸﻨﻣ ﺎﺑ از ﺎﺑ نآ ﻪﺴﯾﺎﻘﻣ و

ﯽﺘﻧآ ﺗﻮﯿﺑ ﯿ ﮏ ﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ و ﻦﯿﺴﯾﺎﻣﻮﮑﻧو يﺎﻫ

ﯽﻌﯿﻤﺳ ﻪﻧﺎﺴﻓا يدﺰﯾ ﯽﯾﺎﺒﻃﺎﺒﻃ هﺪﯾﺮﻓ ،

1

2

ﯽﻧﺎﻬﺒﻬﺑ هداﺰﯿﻠﻋ زوﺮﻬﺑ ،



،

3

ﯽﻧاﺮﻬﻃ يﺮﻫﺎﻈﻣ ﯽﻔﻄﺼﻣ

2

1 - ناﺮﯾا ،ﺪﻬﺸﻣ ،ﺪﻬﺸﻣ ﯽﺳودﺮﻓ هﺎﮕﺸﻧاد ،يزروﺎﺸﮐ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ ﯽﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،يﺮﺘﮐد ﻪﺘﺧﻮﻣآ ﺶﻧاد

2 - ناﺮﯾا ،ﺪﻬﺸﻣ ،ﺪﻬﺸﻣ ﯽﺳودﺮﻓ هﺎﮕﺸﻧاد ،يزروﺎﺸﮐ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ ﯽﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،دﺎﺘﺳا

3 - ،رﺎﯾدﺎﺘﺳا ناﺮﯾا ،ﯽﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽﻌﯿﺒﻃ ﻊﺑﺎﻨﻣ و يزروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و ﯽﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ ﯽﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ

) رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 19 / 05 / 98 رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 20 / 07 / 98 (

هﺪﯿﮑﭼ

ﺶﻫوﮋﭘ ﻦﯾا زا فﺪﻫ

، گﺮﺑ زا ﺲﻧﺎﺳا جاﺮﺨﺘﺳا ﺶﻔﻨﺑ ﻮﺑزﺎﻧ

، ﺗ اﺰﺟا و تﺎﺒﯿﮐﺮﺗ ﯽﯾﺎﺳﺎﻨﺷ ﺪﺿ ﺖﯿﻟﺎﻌﻓ ﯽﺳرﺮﺑ و ﺲﻧﺎﺳا هﺪﻨﻫد ﻞﯿﮑﺸ

ﯾﺮﺘﮐﺎﺑ ﯽﯾﺎ شور ﻪﺑ نآ يﺎﻫ

ﺮﺘﮐﺎﺑ زا يداﺪﻌﺗ ﺮﺑ ﯽﻤﮐ و ﯽﻔﯿﮐ ﻒﻠﺘﺨﻣ ي

يرﺎﻤﯿﺑ ﺺﺧﺎﺷ يﺎﻫ ﯽﺘﻧآ ﺎﺑ نآ ﻪﺴﯾﺎﻘﻣ ﺖﯾﺎﻬﻧ رد و اﺬﻏ از

ﯿﺗﻮﯿﺑ ﮏ ﻂﯾاﺮﺷ رد ﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ و ﻦﯿﺴﯾﺎﻣﻮﮑﻧو يﺎﻫ

ﯽﻫﺎﮕﺸﯾﺎﻣزآ دﻮﺑ

. هﺎﮕﺘﺳد ﻂﺳﻮﺗ ﺲﻧﺎﺳا هﺪﻨﻫد ﻞﯿﮑﺸﺗ ياﺰﺟا ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ

يزﺎﮔ ﯽ ﺪﻧﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ .

ﺿ ﺮﺛا ﺪ ﺲﻧﺎﺳا ﯽﺑوﺮﮑﯿﻣ ﺶﻔﻨﺑ ﻮﺑزﺎﻧ

رد ﮏﻫﺎﭼ شور ﻪﺑ

رﺎﮔآ ﻦﯿﯿﻌﺗ ﺪﺷ ﯽﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﺲﻧﺎﺳا

ﺰﯿﻧ ثاﺮﺑ ﻦﺷﻮﻠﯾادوﺮﮑﯿﻣ زا هدﺎﻔﺘﺳا ﺎﺑ فﺮﻌﻣ و

ﺪﯾاﺮﻠﮐ مﻮﯿﻟوزاﺮﺘﺗ ﻞﯿﻨﻓ يﺮﺗ ﺪﯾدﺮﮔ ﻦﯿﯿﻌﺗ

. داد نﺎﺸﻧ ﺞﯾﺎﺘﻧ

ﻪﮐ 28 عﻮﻤﺠﻣ رد هﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺐﯿﮐﺮﺗ 28

/ 99 ﺮﺗ ﺪﺻرد ﻫد ﯽﻣ ﻞﯿﮑﺸﺗ ار ﺲﻧﺎﺳا تﺎﺒﯿﮐ ﻨ

ﺪ . p-Allylanisole ﺎﺑ

64 / 51 هﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺐﯿﮐﺮﺗ ﻦﯾﺮﺘﺸﯿﺑ

ﺲﻧﺎﺳا زا ﺶﯿﺑ ﯽﯾﺎﻬﻨﺗ ﻪﺑ و ،دﻮﺑ 50

ﯽﻣ ﻞﻣﺎﺷ ار ﺲﻧﺎﺳا ﺐﯿﮐﺮﺗ زا ﺪﺻرد ﺪﺷ

. ﻞﻣﺎﺷ ﺮﮕﯾد ﯽﻠﺻا تﺎﺒﯿﮐﺮﺗ n-Tricosane

) 83 / 24 (%

و Linalool

) 81 / 14 (%

دﻮﺑ . آ رد ﮏﻫﺎﭼ شور رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻂﺳﻮﺘﻣ ﺮﺘﮐﺎﺑ ﺮﺑ رﺎﮔ

ي ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ 9/

15 ﯽﻠﯿﻣ يﺮﺘﮐﺎﺑ ياﺮﺑ و ﺮﺘﻣ ﻫ

ﯽﻔﻨﻣ مﺮﮔ يﺎ 15

/ 11 ﯽﻠﯿﻣ ﺮﺘﻣ

دﻮﺑ . ﺲﻧﺎﺳا ﯽﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﺶﻔﻨﺑ ﻮﺑزﺎﻧ

ﺮﺑ يا يﺮﺘﮐﺎﺑ يﺎﻫ يرﺎﻤﯿﺑ هدوﺪﺤﻣ رد از 6/

4 ﺎﺗ 8/

36 ﯽﻠﯿﻣ ﯽﻠﯿﻣ ﺮﺑ مﺮﮔ دﻮﺑ ﺮﺘﯿﻟ

. ﯽﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ

ﺲﻧﺎﺳا ﺞﻧر رد ﺰﯿﻧ 6/

4 ﺎﺗ 6/

73 ﯽﻠﯿﻣ ﮔ ﯽﻠﯿﻣ ﺮﺑ مﺮ ﺮﺘﯿﻟ دﻮﺑ . ﯽﻣ ﯽﻠﮐ رﻮﻃ ﻪﺑ ﺲﻧﺎﺳا ﻪﮐ ﺖﻔﮔ ناﻮﺗ

ﺶﻔﻨﺑ ﻮﺑزﺎﻧ يﺮﺘﮐﺎﺑ ﺮﺑ

ﻏ رد ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ ﺖﻈﻠ

ﻦﯿﯾﺎﭘ يﺎﻫ ﺮﺗ

ﺖﺴﻧاﻮﺗ و دﻮﺑ ﺮﺛﻮﻣ نآ ﺪﺷر زا

ﺪﻨﮐ يﺮﯿﮔﻮﻠﺟ ﺎﻫ .

ﺪﯿﻠﮐ نﺎﮔژاو : ﺶﻔﻨﺑ ﻮﺑزﺎﻧ

،

،ﯽﺑوﺮﮑﯿﻣﺪﺿ ﺖﯿﻟﺎﻌﻓ ﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ

، ﻖﯿﻗر ،ﻦﯿﺴﯾﺎﻣﻮﮑﻧو ﻊﯾﺎﻣ رد يزﺎﺳ

.

تﺎﺒﺗﺎﮑﻣ لﻮﺌﺴﻣ

:

tabatabai@um.ac.ir

1 - ﻪﻣﺪﻘﻣ

رﺎﻤﯿﺑ ي ﻪﻧﻻﺎﺳ اﺬﻏ زا ﯽﺷﺎﻧ يﺎﻫ 30

% يﺎﻫرﻮﺸﮐ رد ﺖﯿﻌﻤﺟ

ﯽﻣ ﺮﯿﮔرد ار ﻪﺘﻓﺎﯾ ﻪﻌﺳﻮﺗ دزﺎﺳ

1] .[ و ﺖﯿﻔﯿﮐ هزوﺮﻣا زا نﺎﻨﯿﻤﻃا

ﯽﯾاﺬﻏ تﻻﻮﺼﺤﻣ ندﻮﺑ ﻢﻟﺎﺳ ﯽﻧاﺮﮕﻧ زا

ﺖﻌﻨﺻ رد ﯽﻠﺻا يﺎﻫ

ﺖﺳاﺬﻏ 2]

.[ يﺮﺘﮐﺎﺑ ﺪﺷر زا يﺮﯿﮔﻮﻠﺟ يﺎﻫ

و دﺎﺴﻓ ﻞﻣﺎﻋ

ﯽﯾاﺬﻏ ﺖﯿﻣﻮﻤﺴﻣ ﯽﺑوﺮﮑﯿﻣ دﺎﺴﻓ ﻦﺘﺧاﺪﻧا ﺮﯿﺧﺎﺗ ﻪﺑ و

ﻮﺼﺤﻣ تﻻ

ﯽﯾاﺬﻏ ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ترﻮﺻ ﻪﺑ ﻪﮐ ﺪﻨﺘﺴﻫ ﯽﯾﺎﻫرﻮﺘﮐﺎﻓ ، يا

ﯽﻣ يرﺎﮔﺪﻧﺎﻣ نﺎﻣز ﺪﻨﻧاﻮﺗ ﺪﻨﻫد ﺶﯾاﺰﻓا ار

3] و 4 .[

ﺖﯿﻤﻫا هدﺎﻔﺘﺳا زا نﺎﻫﺎﯿﮔ ﯾوراد ﯽﻌﯿﺒﻃ و ﯽ زا يﺮﯿﮕﺸﯿﭘ رد

يرﺎﻤﯿﺑ ،ﺎﻫ نﺎﻣرد و ﺖﻌﻧﺎﻤﻣ زا ﺪﺷر يﺮﺘﮐﺎﺑ يﺎﻫ يرﺎﻤﯿﺑ از ﻪﺑ

ﻪﺘﺧﺎﻨﺷ ﯽﺑﻮﺧ هﺪﺷ

ﺖﺳا . ﺎﺑ دﻮﺟو عﻮﻨﺗ ﺘﺴﮔ و شﺮ رﺎﯿﺴﺑ يدﺎﯾز

ﻪﮐ ﯽﯾوراد نﺎﻫﺎﯿﮔ رد

و ناﺮﯾا نﺎﻬﺟ يﺎﻫرﻮﺸﮐ ﺮﯾﺎﺳ ﺪﻧراد

ﺶﻫوﮋﭘ ﯽﻧاوﺮﻓ يﺎﻫ رد

ﻪﻨﯿﻣز ﺪﺿ ﺮﺛا ﯽﺳرﺮﺑ و ﯽﺑوﺮﮑﯿﻣ

ﯽﺘﻧآ نﺎﻫﺎﯿﮔ ﻦﯾا ﯽﻧاﺪﯿﺴﮐا نﺎﻨﭽﻤﻫ

ﻪﻣادا دراد 5] .[ رد لﺎﺣ ﺮﺿﺎﺣ

ﺖﻣوﺎﻘﻣ يﺮﺘﮐﺎﺑ ﺎﻫ ي يرﺎﻤﯿﺑ ﯽﺘﻧآ ﻪﺑ ﺖﺒﺴﻧ از ﺞﯾار يﺎﻫ ﮏﯿﺗﻮﯿﺑ

ﯽﻧﺎﻣرد ﻪﺑ ﮏﯾ ﻞﮑﺸﻣ گرﺰﺑ ﯽﺳﺎﺳا ، ﺮﺳﺎﺗﺮﺳ رد هﺪﻨﻨﮐ ﺪﯾﺪﻬﺗ و

ﻞﯾﺪﺒﺗ نﺎﻬﺟ هﺪﺷ

ﺖﺳا ﻪﮐ يرﻮﻃ ﻪﺑ رﺎﻌﺷ

لﺎﺳ 2011 نﺎﻣزﺎﺳ

ﺖﺷاﺪﻬﺑ ﯽﻧﺎﻬﺟ

"

ﺖﻣوﺎﻘﻣ ﻪﺑ يﺎﻫوراد ﺪﺿ ﯽﺑوﺮﮑﯿﻣ ﮏﯾ ﺪﯾﺪﻬﺗ

ﯽﻧﺎﻬﺟ

"

ﯽﻣ ﺪﺷﺎﺑ .

تﺎﺒﯿﮐﺮﺗ ﯽﺑوﺮﮑﯿﻣﺪﺿ ﯽﮑﯾ ﯽﯾوراد نﺎﻫﺎﯿﮔ

زا ﻊﺑﺎﻨﻣ ﺎﺑ و ﺪﻨﻤﺷزرا

ﺖﯿﻤﻫا ﺰﺋﺎﺣ رد

ﻢﻠﻋ ،ﯽﮑﺷﺰﭘ ﺖﻌﻨﺻ يزﺎﺳوراد و

ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ

ﻪﺑ رﺎﻤﺷ ﯽﻣ ﺪﻨﯾآ . ﻪﺟﻮﺗ ﺎﺑ شﺮﺘﺴﮔ ﻪﺑ يرﺎﻤﯿﺑ

يﺎﻫ زا ﯽﺷﺎﻧ ﺖﻧﻮﻔﻋ

ﯽﯾاﺬﻏ ﺖﯿﻣﻮﻤﺴﻣ و ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ ﺖﻣوﺎﻘﻣ و

يرﺎﻤﯿﺑ يﺎﻫ ،از

ﯽﯾﺎﺳﺎﻨﺷ ﺮﺘﺸﯿﺑ ﻪﭼ ﺮﻫ ﯽﻌﯿﺒﻃ و ﯽﯾوراد نﺎﻫﺎﯿﮔ

رد و لﺮﺘﻨﮐ نﺎﻣرد

نارﺎﻤﯿﺑ ﺮﺛﻮﻣ ﺪﻫاﻮﺧ دﻮﺑ 6] .[

ﺮﺑ نوﺰﻓا 50

ﯽﺘﻧآ فﺮﺼﻣ زا ﻪﮐ ﺖﺳا لﺎﺳ ﮏﯿﺗﻮﯿﺑ

ﺞﯾار يﺎﻫ

ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ لﺮﺘﻨﮐ و نﺎﻣرد ياﺮﺑ ﯽﻧﺎﻣرد و ﺖﻧﻮﻔﻋ ﻞﻣﺎﻋ يﺎﻫ

ﯽﻣ ﺖﯿﻣﻮﻤﺴﻣ هدﺎﻔﺘﺳا ﯽﻟو ،درﺬﮔ

ﮏﺷﺰﭘ ﺰﯾﻮﺠﺗ نوﺪﺑ و رﺮﮑﻣ يﺎﻫ

ﮏﯿﺗﻮﯿﺑ ﯽﺘﻧآ ﻪﺑ ﺖﻣوﺎﻘﻣ هﺪﯾﺪﭘ زوﺮﺑ ﺚﻋﺎﺑ ﻦﯾا نﺎﻣرد و هﺪﺷ ﺎﻫ

زا ﻪﻧﻮﮔ ﻪﯾﻮﺳ يرﺎﻤﯿﺑ يﺎﻫ ﺖﺳا ﻪﺘﺧﺎﺳ ﻪﺟاﻮﻣ ﻞﮑﺸﻣ ﺎﺑ ار از

7] .[ ﺶﻔﻨﺑ ﻮﺑزﺎﻧ

ﺎﯾ ﺎﺤﯾر ن ﺶﻔﻨﺑ ﯽﻣ ﺪﻨﻤﺷزرا ﯽﯾوراد نﺎﻫﺎﯿﮔ زا ﯽﮑﯾ

يﺎﻫ لﻮﻠﺳ رد ﺲﻧﺎﺳا يدﺎﯾز راﺪﻘﻣ ندﻮﺑ اراد ﺐﺒﺳ ﻪﺑ ﻪﮐ ﺪﺷﺎﺑ دراد ﯽﺘﻨﺳ ﺐﻃ رد ﯽﻧاواﺮﻓ دﺮﺑرﺎﮐ ﯽﺸﯾور .

ﻮﺑزﺎﻧ ﺶﻔﻨﺑ هداﻮﻧﺎﺧ زا

ﯽﻣ نﺎﯿﻋﺎﻨﻌﻧ ﺪﺷﺎﺑ

. ﻮﺑزﺎﻧ ﺶﻔﻨﺑ يﺮﯿﺴﻣﺮﮔ ﻖﻃﺎﻨﻣ رد ﯽﻌﯿﺒﻃ رﻮﻃ ﻪﺑ

ﺪﻨﮐ ﯽﻣ ﺪﺷر يﺮﯿﺴﻣﺮﮔ ﻪﻤﯿﻧ و .

ﻮﺑزﺎﻧ د ناﺮﯾا رد طﺎﻘﻧ ﯽﻣﺎﻤﺗ ر

ﯽﻣ ﺖﺸﮐ دﻮﺷ . هﺎﯿﮔ گﺮﺑ ﻮﺑزﺎﻧ ود رد ﯽﻣ هﺪﯾد ﺶﻔﻨﺑ و ﺰﺒﺳ عﻮﻧ -

گﺮﺑ ﻪﺑ ﮓﻧر ﺶﻔﻨﺑ يﺮﻫﺎﻇ ﻦﯿﻧﺎﯿﺳﻮﺘﻧآ دﻮﺟو ﻞﯿﻟد ﻪﺑ ﻪﮐ دﻮﺷ ﺎﻫ

ﯽﻣ ﺪﻫد 9] و 8 . [ ﻮﺑزﺎﻧ نﺎﻣرد ﺖﻬﺟ ﯽﺘﻨﺳ رﻮﻃ ﻪﺑ و ﻢﯾﺪﻗ مﺎﯾا زا

يرﺎﻤﯿﺑ ﻪﻓﺮﺳ ،دردﺮﺳ يﺎﻫ

، و ﻞﮕﻧا ،ﻞﯿﮔز ،لﺎﻬﺳا ﯽﺘﺣارﺎﻧ

يﻮﯿﻠﮐ

ﯽﻣ هدﺎﻔﺘﺳا هﺪﺷ

ﺖﺳا . ناﺮﯾا ﻖﻃﺎﻨﻣ زا ﯽﺧﺮﺑ رد ﯽﺘﻨﺳ رﻮﻃ ﻪﺑ

نورزﺎﮐ ﺪﻨﻧﺎﻤﻫ )

سرﺎﻓ نﺎﺘﺳا (

نﺎﺘﺴﺘﺷد و )

ﺮﻬﺷﻮﺑ نﺎﺘﺳا (

ﻦﯾا زا

،نﺎﻫد ﻢﺧز مﺎﯿﺘﻟا ياﺮﺑ هﺎﯿﮔ درﻮﻣ هﺪﻌﻣ هﺪﻨﻨﮐ ﺖﯾﻮﻘﺗ و ﺐﺗ ﺶﻫﺎﮐ

ﯽﻣ راﺮﻗ هدﺎﻔﺘﺳا دﺮﯿﮔ

10] .[

ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ ﯽﻣﻮﺑ ندﻮﺑ هﺎﯿﮔ ﻮﺑزﺎﻧ ﺶﻔﻨﺑ رد ﯽﺳﺮﺘﺳد ،ناﺮﯾا

،نﺎﺳآ

نازرا و فﺮﺼﻣ ﯽﯾاﺬﻏ و ﯽﯾوراد ﻦﯾا هﺎﯿﮔ زا نﺎﻣز يﺎﻫ رود رد

ناﺮﯾا ﻦﯾا ﺶﻫوﮋﭘ ﯽﻣ ﺪﻧاﻮﺗ ﻪﻣﺪﻘﻣ يا ﺖﻬﺟ هدﺎﻔﺘﺳا ﯽﻠﻤﻋ زا

ﺲﻧﺎﺳا ﻦﯾا هﺎﯿﮔ رد ﻊﯾﺎﻨﺻ ﯽﯾاﺬﻏ ﺪﺷﺎﺑ . ﺶﻫوﮋﭘ ﻦﯾا زا فﺪﻫ

،

گﺮﺑ زا ﺲﻧﺎﺳا جاﺮﺨﺘﺳا ﻮﺑزﺎﻧ

،ﺶﻔﻨﺑ اﺰﺟا و تﺎﺒﯿﮐﺮﺗ ﯽﯾﺎﺳﺎﻨﺷ

ﺲﻧﺎﺳا هﺪﻨﻫد ﻞﯿﮑﺸﺗ و

ﺖﯿﻟﺎﻌﻓ ﯽﺳرﺮﺑ ﺪﺿ

يﺮﺘﮐﺎﺑ نآ ﻪﺑ شور

ﺮﺘﮐﺎﺑ زا يداﺪﻌﺗ ﺮﺑ ﯽﻤﮐ و ﯽﻔﯿﮐ ﻒﻠﺘﺨﻣ يﺎﻫ ي

ﺺﺧﺎﺷ يﺎﻫ

يرﺎﻤﯿﺑ ﯽﺘﻧآ زا يداﺪﻌﺗ ﺎﺑ نآ ﻪﺴﯾﺎﻘﻣ ﺖﯾﺎﻬﻧ رد و اﺬﻏ از ﮏﯿﺗﻮﯿﺑ

يﺎﻫ

ﯽﻧﺎﻣرد ﺞﯾار ﻂﯾاﺮﺷ رد

ﯽﻫﺎﮕﺸﯾﺎﻣزآ دﻮﺑ

.

2 - شور و داﻮﻣ ﺎﻫ

2 - 1 - ﯽﯾﺎﯿﻤﯿﺷ داﻮﻣ

، ﯽﺑوﺮﮑﯿﻣ ﺖﺸﮐ ﻂﯿﺤﻣ و

هﺎﮕﺘﺳد هدﺎﻔﺘﺳا درﻮﻣ يﺎﻫ

داﻮﻣ ﻂﯿﺤﻣ ،ﯽﯾﺎﯿﻤﯿﺷ ﯽﺑوﺮﮑﯿﻣ ﺖﺸﮐ يﺎﻫ

هﺎﮕﺘﺳد و يﺎﻫ درﻮﻣ

ﻞﻣﺎﺷ ﺶﻫوﮋﭘ ﻦﯾا رد هدﺎﻔﺘﺳا :

ﺮﺗاو نﻮﺘﭙﭘ )

BDH Chemicals

Ltd., England (

ﺮﮕﻨﯾر لﻮﻠﺤﻣ ، )

Merck, Germany (

،

ﻦﻤﺗاو ﺬﻏﺎﮐ )

WhatmanNo. 3 filter paper, Whatman

Ltd., England (

ﯽﮕﻧﺮﺳ ﺮﺳ ﺮﺘﻠﯿﻓ ، 2/

0 ،ﺮﺘﻣوﺮﮑﯿﻣ ﺖﯿﻠﭘ

96

ﻪﻧﺎﺧ

، ﻨﯿﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂﯿﺤﻣ رﺎﮔآ نﻮﺘﻨﯿﻫ ﺮﻟﻮﻣ و ثاﺮﺑ نﻮﺘ

) ﺖﺧﺎﺳ

DEFCO Laboratories, USA (،

ﯽﻫﺎﮕﺸﯾﺎﻣزآ بﺎﯿﺳآ )

لﺪﻣ

Warning نﺎﻤﻟآ ﺖﺧﺎﺳ

( ، ﺎﺘﯿﺠﯾد يوزاﺮﺗ ل

) EK 300i ﺖﻗد ﺎﺑ

01 /0 ( ، رﻮﺗﺎﺑﻮﮑﻧا ) Flash shaker (

، يراﺪﻬﮕﻧ ﺖﻬﺟ رﻮﺗﺎﺑﻮﮑﻧا

ﺖﺸﮐ وﻼﮐﻮﺗا ،ﺐﺳﺎﻨﻣ ﻂﯾاﺮﺷ رد ﺎﻫ )

لﺪﻣ Memmert (

،

ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا Sigma

، ﻦﺑ يرﺎﻣ ) لﺪﻣ Memmert (

ﺎﯾ نوآ ،

رﻮﻓ ) لﺪﻣ Memmert (

ﺮﯿﻐﺘﻣ ﺮﻠﭙﻤﺳ ، 10

ﺎﺗ 1000 ﺮﺘﯿﻟوﺮﮑﯿﻣ

) فورﺪﻨﭘا ( و ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ و يﺎﻫ ﻧ ﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ و ﻦﯿﺴﯾﺎﻣﻮﮑ )

ﻦﺗدﺎﭘ

ﺐﻃ ( دﻮﺑ .

2 - 2 - ﻪﯾﻮﺳ ﻪﯿﻬﺗ ﯽﺑوﺮﮑﯿﻣ يﺎﻫ

لﺎﻌﻓ و نآ يزﺎﺳ ﺎﻫ

ﯽﺴﮐﻮﮐ ﺖﺒﺜﻣ مﺮﮔ يﺮﺘﮐﺎﺑ ﻪﻧﻮﮔ ود ﺶﻫوﮋﭘ ﻦﯾا رد ﺋروا سﻮﮐﻮﮐﻮﻠﯿﻓﺎﺘﺳا) و سﻮ

ﺰﻧژﻮﯿﭘ سﻮﮐﻮﮐﻮﺘﭘﺮﺘﺳا (

ﻪﻧﻮﮔ ود و

ﻞﯿﺳﺎﺑ ﯽﻔﻨﻣ مﺮﮔ يﺮﺘﮐﺎﺑ ﯽﻠﮐ ﺎﯿﺷﺮﺷا)

ازﻮﻨﯾژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ و (

راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﺖﻓﺮﮔ

. يﺮﺘﮐﺎﺑ يوﺎﺣ ﻪﻟﻮﻟ ﻼﻣﺎﮐ ﻂﯾاﺮﺷ رد ﺎﻫ

ﯽﭘ ﻂﺳﻮﺗ ﺲﭙﺳ ،ﺪﺷ زﺎﺑ ﻞﯾﺮﺘﺳا تﺎﺒﯿﮐﺮﺗ زا رﻮﺘﺳﺎﭘ ﺖﭘ

يﺮﺘﮐﺎﺑ نﻮﯿﺴﻧﺎﭙﺳﻮﺳ ﺖﺸﮐ ﻂﯿﺤﻣ ﻪﺑ ﺎﻫ

ﺮﻟﻮﻣ ﺪﻣﺎﺟ و ﻊﯾﺎﻣ يﺎﻫ

ﻨﯿﻫ يﺮﺘﮐﺎﺑ و ﺪﯾدﺮﮔ ﺢﯿﻘﻠﺗ دﻮﺑ هﺪﺷ هدﺎﻣآ ﻞﺒﻗ زا ﻪﮐ نﻮﺘ ﻪـﺑ ﺎﻫ

تﺪﻣ 24 يﺎﻣد رد ﺖﻋﺎﺳ 37

ﻪﺟرد ﯽﺘﻧﺎﺳ داﺮﮔ ﻪﻧﺎﺨﻣﺮﮔ يراﺬﮔ

ﺪﻧﺪﺷ . لﺎﻌﻓ ﺖﻬﺟ يﺮﺘﮐﺎﺑ يزﺎﺳ

ﺎﻫ 24 مﺎﺠﻧا زا ﻞﺒﻗ ﺖﻋﺎﺳ

ﺶﯾﺎﻣزآ ﺪﺿ ﺖﯿﻟﺎﻌﻓ ﯽﺑﺎﯾزرا يﺎﻫ ﯽﯾﺎﯾﺮﺘﮐﺎﺑ

ﻞﯾﺮﺘﺳا ﺲﻧآ ﮏﻤﮐ ﻪﺑ ،

هﺮﯿﺧذ ﺖﺸﮐ زا )

ردﺎﻣ ( ﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂﯿﺤﻣ ﻪﺑ مﺎﺠﻧا ﺢﯿﻘﻠﺗ نﻮﺘﻨﯿ

ﺪﺷ . ﺮﮕﻨﯾر لﻮﻠﺤﻣ زا تروﺪﮐ نﺪﺷ ﺮﺑاﺮﺑ ﺖﻬﺟ

ﺪﺷر

ﮏﻣ ﻢﯿﻧ دراﺪﻧﺎﺘﺳا ﺎﺑ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ ﺪﻨﻟرﺎﻓ

ﺪﺷ هدﺎﻔﺘﺳا .

ﻦﯾا رد

يﺮﺘﮐﺎﺑ ناﺰﯿﻣ ﺖﻟﺎﺣ ﺎﺑ ﺮﺑاﺮﺑ ﺎﻫ

) Colony Forming Unit/mL

10⁸

× 5/

1 ( دﻮﺑ 11] .[

2 - 3 - يروآ ﻊﻤﺟ ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ ﺲﻧﺎﺳا جاﺮﺨﺘﺳا و

گﺮﺑ ﻮﺑزﺎﻧ ﺶﻔﻨﺑ زا ﺸﻣ ﺪﻬ ) يﻮﺿر نﺎﺳاﺮﺧ (

ﺪﺷ يراﺪﯾﺮﺧ .

راﺪﻘﻣ

50 مﺮﮔ زا ﻮﺑزﺎﻧ ﺶﻔﻨﺑ ﺎﺑ هاﺮﻤﻫ 750 ﯽﻠﯿﻣ ﻪﺑ ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟ هﺎﮕﺘﺳد

ﺮﺠﻧﻮﻠﮐ ﻪﺸﯿﺷ يا ﺖﺧﺎﺳ ﺖﮐﺮﺷ كادآ ﻪﮐ سﺎﺳا رﺎﮐ نآ ﺮﯿﻄﻘﺗ ﯽﺑآ

ﺖﺳا ﻞﻘﺘﻨﻣ و ﻪﺑ تﺪﻣ 3 ﺖﻋﺎﺳ ﺎﺑ ﺖﻋﺮﺳ ﮏﯾ ﯽﻠﯿﻣ ﺮﺘﯿﻟ رد

ﻪﻘﯿﻗد ﻞﻤﻋ ﺲﻧﺎﺳا يﺮﯿﮔ مﺎﺠﻧا ﺖﻓﺮﮔ . ﺲﻧﺎﺳا ﺎﺑ تﺎﻔﻟﻮﺳ ﺳ

،ﻢﯾﺪ

يﺮﯿﮕﺑآ و رد فﺮﻇ

،ﻞﯾﺮﺘﺳا رود زا رﻮﻧ و رد لﺎﭽﺨﯾ يراﺪﻬﮕﻧ

ﺪﺷ 12] . [ ﻞﮑﺷ رد 1 ﻪﻧﻮﻤﻧ ، هﺪﺷ هداد نﺎﺸﻧ ﺶﻔﻨﺑ ﻮﺑزﺎﻧ هﺎﯿﮔ زا يا

ﺖﺳا .

Fig 1 Purple basil.

2 - 4 - ﺲﻧﺎﺳا ﯽﯾﺎﯿﻤﯿﺷ تﺎﺒﯿﮐﺮﺗ ﯽﯾﺎﺳﺎﻨﺷ ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ

ﯽﯾﺎﺳﺎﻨﺷ تﺎﺒﯿﮐﺮﺗ ﺲﻧﺎﺳا ﻮﺑزﺎﻧ ،ﯽﻫﺎﯿﮔ مﻮﻠﻋ هﺪﮑﺸﻫوﮋﭘ رد ﺶﻔﻨﺑ

ﮕﺸﻧاد ﺪﺷ مﺎﺠﻧا ﯽﺘﺸﻬﺑ ﺪﯿﻬﺷ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎ .

ﺲﻧﺎﺳا جاﺮﺨﺘﺳا

هﺪﺷ ﺎﺑ ﻖﯾرﺰﺗ 2/

0 هﺎﮕﺘﺳد ﻪﺑ ﺮﺘﯿﻟوﺮﮑﯿﻣ زﺎﮔ

ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ ﻞﺼﺘﻣ

ﻪﺑ ﻒﯿﻃ رﺎﮕﻧ لﺪﻣ ﯽﻣﺮﺟ TRACE MS

) هﺪﻧزﺎﺳ ﺖﮐﺮﺷ :

ThermoQ

ً

uest-Finnigan (

يوﺎﺣ نﻮﺘﺳ DB-5 لﻮﻃ 30

،ﺮﺘﻣ ﺮﻄﻗ ﯽﻠﺧاد 25 /0 ﺮﺘﻣ ﯽﻠﯿﻣ و

ﺖﻣﺎﺨﺿ ﻦﮐﺎﺳ زﺎﻓ 25

/ 0

ﺘﻣوﺮﮑﯿﻣ ﺮ ﻞﺼﺘﻣ ﻪﺑ ﻒﯿﻃ ﺞﻨﺳ ﯽﻣﺮﺟ لﺪﻣ Quadrupole مﺎﺠﻧا

ﺖﻓﺮﯾﺬﭘ . يﺎﻣد نﻮﺘﺳ زا 40 ﻪﺟرد ﯽﺘﻧﺎﺳ داﺮﮔ ﺎﺗ 250 ﻪﺟرد

ﯽﺘﻧﺎﺳ داﺮﮔ ﺖﻋﺮﺳ ﺎﺑ 5/

2 ﯽﺘﻧﺎﺳ ﻪﺟرد ﺖﻓﺎﯾ ﺶﯾاﺰﻓا ﻪﻘﯿﻗد ﺮﺑ داﺮﮔ

.

ﺖﻋﺮﺳ ﺎﺑ ﻢﯿﻠﻫ زﺎﮔ زا 1/

1 يژﺮﻧا زا و ﻪﻘﯿﻗد رد ﺮﺘﯿﻟ ﯽﻠﯿﻣ

نﻮﯿﺳاﺰﯿﻧﻮﯾ 70

دﺎﻔﺘﺳا نوﺮﺘﮑﻟا ه

ﺪﺷ 13] .[

2 - 5 - ﻪﺒﺳﺎﺤﻣ ﺺﺧﺎﺷ

يرادزﺎﺑ و

ﯽﯾﺎﺳﺎﻨﺷ

ﺎﻫ ﺐﯿﮐﺮﺗ

ﻪﺒﺳﺎﺤﻣ ياﺮﺑ ﺲﯾﺪﻧا

يﺎﻫ يرادزﺎﺑ

،تﺎﺒﯿﮐﺮﺗ يﺎﻫ نﺎﮑﻟآ لﺎﻣﺮﻧ

C22– C9 ﻖﯾرﺰﺗ

ﺪﯾدﺮﮔ . ﯽﯾﺎﺳﺎﻨﺷ ﺐﯿﮐﺮﺗ ﺎﻫ ﺎﺑ ﻪﻌﻟﺎﻄﻣ

ﻒﯿﻃ يﺎﻫ ﯽﻣﺮﺟ و ﻪﺴﯾﺎﻘﻣ ﺎﺑ ﻒﯿﻃ ﯽﻣﺮﺟ ﺐﯿﮐﺮﺗ

،دراﺪﻧﺎﺘﺳا يﺎﻫ

ﺎﺑ هدﺎﻔﺘﺳا زا تﺎﻋﻼﻃا دﻮﺟﻮﻣ رد ﻪﻧﺎﺨﺑﺎﺘﮐ ﺮﺗﻮﯿﭙﻣﺎﮐ

و ﻪﺑ ﮏﻤﮐ

ﺺﺧﺎﺷ يﺎﻫ ﻪﺒﺳﺎﺤﻣ يرادزﺎﺑ ﺪﺷ

. ﻪﺴﯾﺎﻘﻣ نآ ﺎﻫ ﺎﺑ ﺺﺧﺎﺷ يﺎﻫ

يرادزﺎﺑ ﻪﮐ دراﺪﻧﺎﺘﺳا رد

ﻊﺑﺎﻨﻣ ﻒﻠﺘﺨﻣ ﺮﺸﺘﻨﻣ

،هﺪﯾدﺮﮔ مﺎﺠﻧا ﺪﺷ .

ﯽﻤﮐ تﺎﺒﺳﺎﺤﻣ )

ﺐﯿﮐﺮﺗ ﺮﻫ ﺪﺻرد (

ﺢﻄﺳ ندﺮﮐ لﺎﻣﺮﻧ شور ﻪﺑ

1

ﺪﺷ مﺎﺠﻧا .

2 - 6 - ﻪﺑ ﯽﺑوﺮﮑﯿﻣﺪﺿ ﺖﯿﻟﺎﻌﻓ ﯽﺳرﺮﺑ نﻮﻣزآ

ﮏﻫﺎﭼ دﺎﺠﯾا شور

يﺮﺘﮐﺎﺑ اﺪﺘﺑا درﻮﻣ يﺎﻫ

ﻪﺑ ،ﯽﺳرﺮﺑ ﺮﮕﻨﯾر لﻮﻠﺤﻣ ﮏﻤﮐ

ﺎﺗ ﺮﺑاﺮﺑ

ﺎﺑ نﺪﺷ دراﺪﻧﺎﺘﺳا تروﺪﮐ 5/

0 ﮏﻣ ،ﺪﻨﻟرﺎﻓ ﻖﯿﻗر ﺪﺷ . ﻂﺳﻮﺗ

زا ﻞﯾﺮﺘﺳا پاﻮﺳ يﺮﺘﮐﺎﺑ

ﺎﻫ ﺖﺸﮐ ﻂﯿﺤﻣ يور و ﺪﺷ ﻪﺘﺷادﺮﺑ ﺮﻟﻮﻣ

رﺎﮔآ نﻮﺘﻨﯿﻫ ﻪﻠﯿﻣ ﻂﺳﻮﺗ ،

ﻪﺸﯿﺷ ي يا يﺮﺘﭘ ﺢﻄﺳ رد ﺪﻨﻧﺎﻣ L ﺶﯾد

8 ﺮﺘﻣ ﯽﺘﻧﺎﺳ ﺪﺷ هداد ﺖﺸﮐ

. ﻞﯾﺮﺘﺳا رﻮﺘﺳﺎﭘ ﺖﭙﯿﭘ و ﻼﺧ ﻂﺳﻮﺗ ،

ﮏﻫﺎﭼ ﺮﻄﻗ ﻪﺑ ﯽﯾﺎﻫ 6

ﯽﻠﯿﻣ ﺪﯾدﺮﮔ دﺎﺠﯾا ﺖﺸﮐ ﻂﯿﺤﻣ يور ﺮﺘﻣ .

ﮏﻫﺎﭼ يﺎﻬﺘﻧا ﺎـﺑ ﺎﻫ

10 ذﻮﻔﻧ زا ﺎﺗ ﺪﺷ ﺮﭘ ﺖﺸﮐ ﻂﯿﺤﻣ ﺮﺘﯿﻟوﺮﮑﯿﻣ

ﯽﻟﺎﻤﺘﺣا ﺲﻧﺎﺳا ﻮﺑزﺎﻧ ﺶﻔﻨﺑ ﻒﮐ ﻪﺑ يﺮﺘﭘ ﺮﻫ زوﺮﺑ زا و يﺮﯿﮔﻮﻠﺟ

دﻮﺷ يﺮﯿﮕﺸﯿﭘ ﺎﻄﺧ ﻪﻧﻮﮔ .

ﻞﺧاد ﺮﻫ ﮏﻫﺎﭼ ﺎﺑ ﺮﻠﭙﻤﺳ 20

ﺖﻗر زا ﺮﺘﯿﻟوﺮﮑﯿﻣ يﺎﻫ

1 ، 2/

4،

1/

1 ، 8/

1 ، 16 /1 ، 32 / 1 و 64 / 1

لﻮﻠﺤﻣ رد نﺎﺤﯾر ﺲﻧﺎﺳا ﺪﯾﺎﺴﮐﻮﻔﻟﻮﺳ ﻞﯿﺘﻣ يد

ﻪﺑ رﻮﻃ ﻪﻧﺎﮔاﺪﺟ

راﺮﻗ هداد ﺪﺷ . ﺮﻫ رد ﺶﯾد يﺮﺘﭘ ﮏﻫﺎﭼ ﮏﯾ

ﻂﺳو رد ناﻮﻨﻋ ﻪﺑ

ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﺪﻫﺎﺷ .

ﺖﯿﻠﭘ رد هﺪﺷ ﺖﺸﮐ يﺎﻫ يﺎﻣد

37 ﻪﺟرد

ﯽﺘﻧﺎﺳ داﺮﮔ تﺪﻣ ﻪﺑ 24

ﻪﻧﺎﺨﻣﺮﮔ ﺖﻋﺎﺳ ﺪﻧﺪﺷ يراﺬﮔ

. ﺮﻄﻗ

ﻪﻟﺎﻫ ﺎﺑ ﺪﺷر مﺪﻋ يﺎﻫ ﻂﺧ زا هدﺎﻔﺘﺳا

ﺶﮐ ﻖﯿﻗد رﻮﻃ ﻪﺑ هزاﺪﻧا

يﺮﯿﮔ

ﺪﺷ . ﮏﺴﯾد زا ﯽﺘﻧآ يﺎﻫ ﮏﯿﺗﻮﯿﺑ ﻦﺴﯾﺎﻣﻮﮑﻧو و ﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ يﺎﻫ

ﯽﺘﻧآ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ و ﺪﯾدﺮﮔ هدﺎﻔﺘﺳا ﮏﯿﺗﻮﯿﺑ

ﺮﻫ درﻮﻣ رد ﺎﻫ ﻪﺴﯾﺎﻘﻣ هﺪﻣآ ﺖﺳد ﻪﺑ ﺞﯾﺎﺘﻧ ﺎﺑ و ﺪﯾدﺮﮔ ﺺﺨﺸﻣ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ

ﺪﺷ 14] .[

2 - 7 - ﻪﺑ ﯽﺑوﺮﮑﯿﻣﺪﺿ ﺖﯿﻟﺎﻌﻓ ﯽﺳرﺮﺑ نﻮﻣزآ

ﻦﯿﯿﻌﺗ شور ﻞﻗاﺪﺣ

ﺖﻈﻠﻏ رﺎﻬﻣ زا ﯽﮔﺪﻨﻨﮐ ﺪﺷر

ﻪﯾﻮﺳ زا رﻮﻈﻨﻣ ﻦﯾا ياﺮﺑ يﺮﺘﮐﺎﺑ يﺎﻫ

يرﺎﻤﯿﺑ دراﺪﻧﺎﺘﺳا يﺎﻫ ﮏﯾ از

ﺖﺸﮐ 24 ﺪﺷ ﻪﯿﻬﺗ ثاﺮﺑ نﻮﺘﻨﯿﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂﯿﺤﻣ رد ﻪﺘﻋﺎﺳ .

لﺎﯾﺮﺳ ﺲﻧﺎﺳا زا ﯽﻟاﻮﺘﻣ ﺖﻗر يﺎﻫ ﻮﺑزﺎﻧ

و ﻪﯿﻬﺗ ﺶﻔﻨﺑ 100

ﺲﻧﺎﺳا زا ﺮﺘﯿﻟوﺮﮑﯿﻣ ﻮﺑزﺎﻧ

ﺖﯿﻠﭘوﺮﮑﯿﻣ ﻪﺑ يﺎﻫ

96 ﻪﻧﺎﺧ يا ﻼﺒﻗ ﻪﮐ

يوﺎﺣ 10 ﺮﺘﯿﻟوﺮﮑﯿﻣ ﮏﻣ ﻢﯿﻧ تروﺪﮐ ﺎﺑ يﺮﺘﮐﺎﺑ

ﻪﻓﺎﺿا دﻮﺑ ﺪﻨﻟرﺎﻓ

ﺪﺷ . ﺶﯾﺎﻣزآ ﺖﺒﺜﻣ لﺮﺘﻨﮐ ياﺮﺑ ﻪﺑﺎﺸﻣ يﺎﻫ )

ﺮﻟﻮﻣ ﺖﺸﮐ ﻂﯿﺤﻣ

ﺲﻧﺎﺳا نوﺪﺑ يﺮﺘﮐﺎﺑ يوﺎﺣ ثاﺮﺑ نﻮﺘﻨﯿﻫ (

ﯽﻔﻨﻣ لﺮﺘﻨﮐ و )

ﻂﯿﺤﻣ

يﺮﺘﮐﺎﺑ نوﺪﺑ ثاﺮﺑ نﻮﺘﻨﯿﻫ ﺮﻟﻮﻣ ﺖﺸﮐ (

مﺎﺠﻧا ﺰﯿﻧ ﺪﺷ . ﺖﯿﻠﭘ 96

ﻪﻧﺎﺧ تﺪﻣ ﻪﺑ يا 24

رد ﺖﻋﺎﺳ يﺎﻣد رد رﻮﺗﺎﺑﻮﮑﻧا

37 ﻪﺟرد

ﯽﺘﻧﺎﺳ ﺖﻓﺮﮔ راﺮﻗ داﺮﮔ .

ﺖﺷﺬﮔ زا ﺲﭘ 24

يﺮﺗ لﻮﻠﺤﻣ ﺖﻋﺎﺳ

ﻪﻧﺎﺧ ﺮﻫ ﻪﺑ و هﺪﺷ ﻪﯿﻬﺗ ﺪﯾاﺮﻠﮐ مﻮﯿﻟوزاﺮﺘﺗ ﻞﯿﻨﻓ 10

زا ﺮﺘﯿﻟوﺮﮑﯿﻣ

فﺮﻌﻣ ﻦﯾا ﻪﻓﺎﺿا

ﺪﺷ . ﻪﻧﺎﺧ رد هدﺎﺘﻓا قﺎﻔﺗا ﯽﺑوﺮﮑﯿﻣ ﺪﺷر ﻪﮐ ﯽﯾﺎﻫ

ﻢﮐ فﺮﻇ ﺪﺷﺎﺑ دﺎﺠﯾا ﯽﻧاﻮﻏرا ﺎﯾ هﺮﯿﺗ ﺰﻣﺮﻗ ﮓﻧر ،ﺖﻋﺎﺳ ﻢﯿﻧ زا ﺮﺗ

ﯽﻣ دﻮﺷ . ﮓﻧر و هداﺪﻧ يور يﺮﺘﮐﺎﺑ ﺪﺷر نآ رد ﻪﮐ ﯽﺘﻈﻠﻏ ﻦﯿﻟوا

ﻪﺑ ﺪﺸﻧ ﻞﯿﮑﺸﺗ ﺰﻣﺮﻗ ناﻮﻨﻋ

ﯽﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ شراﺰﮔ

ﺪﺷ 15] .[

2 - 8 - ﻞﻗاﺪﺣ ﺖﻈﻠﻏ

ﺪﻨﺸﮐ ﮔ ﻮﺑزﺎﻧ ﺲﻧﺎﺳا ﯽ

ﺶﻔﻨﺑ

ﺲﻧﺎﺳا ﯽﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﯿﯿﻌﺗ ﺶﻔﻨﺑ ﻮﺑزﺎﻧ

ﻦﯿﯿﻌﺗ ﻪﻣادا رد

ﯽﻣ نآ ﻞﻤﮑﻣ و هﺪﻧرادزﺎﺑ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﺪﺷﺎﺑ

. ﻦﯿﯿﻌﺗ ياﺮﺑ ﻞﻗاﺪﺣ

ﯽﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﮏﻫﺎﭼ ﻪﻤﻫ

ﺪﻗﺎﻓ يﺎﻫ ﮓﻧر ﺮﯿﯿﻐﺗ

، ﺢﻄﺳ ﺮﺑ ﻂﯿﺤﻣ

ﺖﺸﮐ ﺖﺸﮐ رﺎﮔآ نﻮﺘﻨﯿﻫ ﺮﻟﻮﻣ هداد

ﺪﺷ . يوﺎﺣ ﺖﺸﮐ ﻂﯿﺤﻣ

يﺮﺘﮐﺎﺑ يﺎﻣد رد 37

ﯽﺘﻧﺎﺳ ﻪﺟرد تﺪﻣ ﻪﺑ داﺮﮔ

24 ﺖﻋﺎﺳ

ﻪﻧﺎﺨﻣﺮﮔ ﺪﺷ يراﺬﮔ .

ﻦﯿﻟوا ﯽﻨﻠﮐ ﭻﯿﻫ ﻪﮐ ﯽﺘﯿﻠﭘ نآ رد

هدﺮﮑﻧ ﺪﺷر

ﻪﺑ ﺪﺷﺎﺑ ناﻮﻨﻋ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ

ﺪﺷ شراﺰﮔ 6]

.[

2 - 9 - رﺎﻣآ ﺰﯿﻟﺎﻧآ ي

ﺶﯾﺎﻣزآ ﯽﻣﺎﻤﺗ ﺎﻫ

ﻪﺳ رد راﺮﮑﺗ ياﺮﺑ ،ﺖﻓﺮﯾﺬﭘ مﺎﺠﻧا ﻪﯾﺰﺠﺗ

و

ﻞﯿﻠﺤﺗ يرﺎﻣآ هداد ﺎﻫ زا مﺮﻧ راﺰﻓا SPSS ﻪﺨﺴﻧ 18 ﺪﺷ هدﺎﻔﺘﺳا .

ﯽﻨﻌﻣ ﺢﻄﺳ ندﻮﺑ راد

5 رد ﺪﺻرد ﺮﻈﻧ

ﺪﺷ ﻪﺘﻓﺮﮔ .

3 - ﺞﯾﺎﺘﻧ ﺚﺤﺑ و

رﻮﻈﻨﻣ ﻪﺑ ﯽﯾوراد نﺎﻫﺎﯿﮔ زا هدﺎﻔﺘﺳا يرﺎﻤﯿﺑ نﺎﻣرد

نﺎﺴﻧا رد يﺎﻫ

ﻪﻘﺑﺎﺳ ﯽﻨﯿﺑ ﺶﯿﭘ ،دراد ﯽﻧﻻﻮﻃ ﺲﺑ يا دروآﺮﺑ و ﺎﻫ

ه هﺪﺷ مﺎﺠﻧا يﺎﻫ

ﯽﻣ نﺎﺸﻧ ﯽﻧﺎﻬﺟ ﺖﺷاﺪﻬﺑ نﺎﻣزﺎﺳ دوﺪﺣ ﻪﮐ ﺪﻫد

80 ﺪﺻرد

ﻪﺒﻨﺟ ياﺮﺑ ﯽﻌﯿﺒﻃ و ﯽﯾوراد نﺎﻫﺎﯿﮔ زا نﺎﻬﺟ ﺖﯿﻌﻤﺟ ﺖﺒﻗاﺮﻣ يﺎﻫ

-

ﯽﻣ هدﺎﻔﺘﺳا دﻮﺧ ﯽﺘﺷاﺪﻬﺑ يﺎﻫ ﺪﻨﯾﺎﻤﻧ

.

ﺲﻧﺎﺳا ﺰﯿﻟﺎﻧآ ﺞﯾﺎﺘﻧ ﻮﺑزﺎﻧ

ﻔﻨﺑ ﺶ هﺎﮕﺘﺳد ﻂﺳﻮﺗ يزﺎﮔ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ

رد لوﺪﺟ 1 ﺖﺳا هﺪﺷ هدروآ ، .

ﯽﯾﺎﺳﺎﻨﺷ ﺐﯿﮐﺮﺗ ﺖﺸﻫ و ﺖﺴﯿﺑ

عﻮﻤﺠﻣ رد هﺪﺷ 28

/ 99 ﺲﻧﺎﺳا تﺎﺒﯿﮐﺮﺗ ﺪﺻرد ﻮﺑزﺎﻧ

ار ﺶﻔﻨﺑ

ﻞﯿﮑﺸﺗ داد .

p-Allylanisole ﺎﺑ

64 / 51 رد هﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺐﯿﮐﺮﺗ ﻦﯾﺮﺘﺸﯿﺑ

زا ﺶﯿﺑ ﯽﯾﺎﻬﻨﺗ ﻪﺑ و ،دﻮﺑ نﺎﺤﯾر ﺲﻧﺎﺳا 50

ﺐﯿﮐﺮﺗ زا ﺪﺻرد

ﺎﺳا ﺪﺷ ﯽﻣ ﻞﻣﺎﺷ ار ﺲﻧ .

ﺶﻫوﮋﭘ رد ﻦﯾا ﺪﺻرد ﺰﯿﻧ ﻪﺑﺎﺸﻣ يﺎﻫ

ﻦﯿﺑ ﺐﯿﮐﺮﺗ 30

ﺎﺗ 70 دﻮﺑ هﺪﺷ شراﺰﮔ ﺪﺻرد ﺮﮐذ ﻪﺑ مزﻻ

ﯽﻣ نﻮﭽﻤﻫ ﯽﻔﻠﺘﺨﻣ ﯽﻣﺎﺳا ياراد ﺐﯿﮐﺮﺗ ﻦﯾا ﻪﮐ ﺪﺷﺎﺑ estragol

،

methoxy-4-(2-propenyl)-benzene ،

estragon و

...

ﯽﻣ ﺪﺷﺎﺑ . ﻞﻣﺎﺷ ﺮﮕﯾد ﯽﻠﺻا تﺎﺒﯿﮐﺮﺗ ﻦﯾا ﺮﺑ هوﻼﻋ n-

Tricosane )

83 / 24 (%

و Linalool )

81 / 14 (%

دﻮﺑ . داﺪﻋا

تﺎﺒﯿﮐﺮﺗ ﯽﻧاوﺮﻓ راﺪﻘﻣ ماﺮﮔﻮﺗﺎﻣوﺮﮐ يدﻮﻤﻋ نﻮﺘﺳ رد جرﺪﻨﻣ ﯽﻣ نﺎﺸﻧ ار ﺶﻔﻨﺑ ﻮﺑزﺎﻧ ﺲﻧﺎﺳا هﺪﻨﻫد ﻞﯿﮑﺸﺗ ﯽﻘﻓا نﻮﺘﺳ و ﺪﻫد

ﯽﯾﺎﺳﺎﻨﺷ و يزﺎﺳاﺪﺟ نﺎﻣز نﻮﺘﺳ رد ﺲﻧﺎﺳا تﺎﺒﯿﮐﺮﺗ زا ﮏﯾ ﺮﻫ

ﯽﻣ نﺎﯿﺑ ار ﺪﻨﮐ

) ﻞﮑﺷ 2 .(

ﻪﻌﻟﺎﻄﻣ رد ﺗ ﻪﮐ يا و ﯽﮔرﺰﺑ رﻮﭘ ﻂﺳﻮ

نارﺎﮑﻤﻫ ) 1386 ( زا ﺶﯿﺑ ﻪﮐ ﺪﯾدﺮﮔ ﺺﺨﺸﻣ ،ﺖﻓﺮﯾﺬﭘ مﺎﺠﻧا ، 50

ﺐﯿﮐﺮﺗ ﻦﯾﺮﺘﺸﯿﺑ و دراد دﻮﺟو نﺎﺤﯾر ﺲﻧﺎﺳا رد ﻒﻠﺘﺨﻣ ﺐﯿﮐﺮﺗ ﺮﮐذ ﻪﺑ مزﻻ ،ﺖﺳا هداد صﺎﺼﺘﺧا دﻮﺧ ﻪﺑ لﻮﮔاﺮﺘﺳا ار ﺲﻧﺎﺳا ﯽﻣ و ﺶﻔﻨﺑ ﻮﺑزﺎﻧ هﺎﯿﮔ ﯽﯾﺎﯿﻤﯿﺷ تﺎﺒﯿﮐﺮﺗ ناﺮﮕﺸﻫوﮋﭘ ﻦﯾا ﻪﮐ ﺪﺷﺎﺑ

ﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ار ﺰﺒﺳ ﺪﻧدﻮﺑ هداد را

10] و 16 . [ ﺶﻫوﮋﭘ ﻪﺠﯿﺘﻧ ﯽﻧاﻮﺨﻤﻫ يدﺎﯾز دوﺪﺣ ﺎﺗ ناﺮﮕﺸﻫوﮋﭘ ﺮﯾﺎﺳ ﺞﯾﺎﺘﻧ ﺎﺑ ﺮﺿﺎﺣ

ﺮﻫ ﺲﻧﺎﺳا ﯽﯾﺎﯿﻤﯿﺷ رﺎﺘﺧﺎﺳ ﻪﮐ ﺖﺳا نآ ﺖﯿﻤﻫا ﺰﺋﺎﺣ ﻪﺘﮑﻧ ،ﺖﺷاد توﺎﻔﺘﻣ ﯽﯾاﻮﻫ و بآ ﻂﯾاﺮﺷ و ﯽﻄﯿﺤﻣ ﯽﻠﻣاﻮﻋ ﺮﯿﺛﺎﺗ ﺖﺤﺗ هﺎﯿﮔ ﯽﻣ ﺪﺷﺎﺑ . Table 1 Chemical compounds of the purple basil

essential oil

No Compound name KI^a %

1 α-Pinene 933 0.09

2 Sabinene 972 0.03

3 β-Pinene 977 0.06

4 6-Methyl-5-hepten-2-one 984 0.08

5 β-Myrcene 988 0.04

6 Limonene 1027 0.05

7 1,8-Cineole 1030 0.57

8 Z-β-Ocimene 1035 0.03

9 E-β-Ocimene 1045 0.13

10 cis-Linalool oxide 1071 0.1

11 trans-Linalool oxide 1088 0.09

12 Linalool 1101 14.81

13 Menthol 1175 0.11

14 Terpineol<alpha-> 1194 0.12

15 p-Allylanisole 1204 51.64

16 Neral 1242 0.31

17 Geranial 1271 0.46

18 trans-caryophyllene 1422 0.32

19 Bergamotene<alpha-trans-> 1437 0.59

20 trans-β-Farnesene 1457 0.3

21 Germacrene D 1484 0.12

22 (E)-α-Bisabolene 1544 1.32

23 n-Octadecane 1797 0.12

24 n-Nonadecane 1897 0.32

25 n-Eicosane 1997 0.43

26 n-Docosane 2197 0.88

27 n-Tricosane 2300 24.83

28 n-Tetracosane 2397 1.33

Fig 2 The chromatogram of purple basil essential oil.

ﺲﻧﺎﺳا ﺮﺛا ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ ﺮﺑ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ

لواﺪﺟ 2 و 3 ﺖﺳا هﺪﺷ هدروآ ، .

ﻪﮐ داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﻦﯾا ﺞﯾﺎﺘﻧ

ﺲﻧﺎﺳا ﻮﺑزﺎﻧ يﺮﺘﮐﺎﺑ ﺪﺷر زا ﯽﺑﻮﺧ ﻪﺑ يرﺎﻤﯿﺑ يﺎﻫ

يﺮﯿﮔﻮﻠﺟ از

نآ ﺮﺛا و هدﺮﮐ ﺮﺘﮐﺎﺑ ﺮﺑ

ي ﻫﺎ ﺮﻣ ﻪﺑ ﺖﺒﺜﻣ مﺮﮔ ﯽﺴﮐﻮﮐ ي ا

ﺮﺘﺸﯿﺑ ﺐﺗ

ﯿﺳﺎﺑ زا ﻞ دﻮﺑ ﯽﻔﻨﻣ مﺮﮔ يﺎﻫ .

ﯽﮔﮋﯾو زا ﯽﮑﯾ ﺲﻧﺎﺳا ﻢﻬﻣ يﺎﻫ

ﺰﯾﺮﮕﺑآ ﺖﯿﺻﺎﺧ ﻪﺑ طﻮﺑﺮﻣ ﯽﯾوراد نﺎﻫﺎﯿﮔ ي

ﯽﻣ نآ ﻦﯾا رد ،ﺪﺷﺎﺑ

ﯽﻣ هﺎﯿﮔ ﺲﻧﺎﺳا ﺖﻟﺎﺣ يﺎﺸﻏ يﺪﯿﭙﯿﻟ ﻪﯾﻻ يور ﺪﻧﻮﯿﭘ ﺎﺑ ﺪﻧاﻮﺗ

ﯽﻟﻮﻠﺳ يرﺪﻨﮐﻮﺘﯿﻣ و يﺮﺘﮐﺎﺑ

ﺎﻫ ﺎﺸﻏ فﺎﮑﺷ ﺚﻋﺎﺑ ي

و ﯽﻟﻮﻠﺳ

لﻮﮑﻟﻮﻣ جوﺮﺧ نﻮﯾ و ﺎﻫ

رد و لﻮﻠﺳ زا جرﺎﺧ ﻪﺑ يﺮﺘﮐﺎﺑ يﺎﻫ

يﺮﺘﮐﺎﺑ گﺮﻣ ﺖﯾﺎﻬﻧ ددﺮﮔ

. ﺪﺿ ﺖﯿﻟﺎﻌﻓ ﺲﻧﺎﺳا ﯽﯾﺎﯾﺮﺘﮐﺎﺑ

ﻪﯿﻠﻋ ﺎﻫ

هدﺮﺘﺴﮔ ﻒﯿﻃ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ زا يا

يرﺎﻤﯿﺑ ﺺﺧﺎﺷ يﺎﻫ ﻞﻣﺎﻋ و از

يﺮﺘﮐﺎﺑ ﻪﻠﻤﺟ زا ،ﺖﻧﻮﻔﻋ و ﺖﯿﻣﻮﻤﺴﻣ ﯽﻔﻨﻣ مﺮﮔ و ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ

ﺖﺳا هﺪﺷ تﺎﺒﺛا .

ﺖﯿﻟﺎﻌﻓ ﺎﻫ ﺪﺿ ي ﺲﻧﺎﺳا ﯽﺑوﺮﮑﯿﻣ يداﺪﻌﺗ ﻪﺑ ،ﺎﻫ

هوﺮﮔ و ﯽﻠﻨﻓ و يﺪﯿﺋﻮﻨﭘﺮﺗ تﺎﺒﯿﮐﺮﺗ زا رد دﻮﺟﻮﻣ لﺎﻌﻓ يﺎﻫ

ﺲﻧﺎﺳا نآ ﻦﯿﺑ تﻻﺎﻌﻔﻧا و ﺎﻫ ﯽﻣ هداد ﺖﺒﺴﻧ ،ﺎﻫ

دﻮﺷ 17] .[

ﺖﯿﻤﻫا هدﺎﻔﺘﺳا زا نﺎﻫﺎﯿﮔ ﯽﻌﯿﺒﻃ و ﯽﯾوراد زا يﺮﯿﮕﺸﯿﭘ رد

يرﺎﻤﯿﺑ و نﺎﻣرد ،ﺎﻫ ﺖﻌﻧﺎﻤﻣ

زا ﺪﺷر يﺮﺘﮐﺎﺑ يﺎﻫ يرﺎﻤﯿﺑ از ﻪﺑ ﺷ ﯽﺑﻮﺧ

ﻪﺘﺧﺎﻨ هﺪﺷ ﺖﺳا . ﻪﻌﻟﺎﻄﻣ رد ﻪﮐ يا ترﻮﺑ ) 2004 (،

مﺎﺠﻧا

ﻪﮐ دﻮﻤﻧ نﺎﯿﺑ داد يﺮﺘﮐﺎﺑ

يﺎﻫ مﺮﮔ ﺖﺒﺜﻣ ﻪﺑ ﺖﺒﺴﻧ يﺮﺘﮐﺎﺑ

يﺎﻫ

مﺮﮔ ﯽﻔﻨﻣ ﺖﯿﺳﺎﺴﺣ يﺮﺘﺸﯿﺑ

تﺎﺒﯿﮐﺮﺗ ﻪﺑ ﺖﺒﺴﻧ ﺲﻧﺎﺳا

ﯽﻫﺎﯿﮔ

ﺪﻧراد . ﻪﮑﻨﯾا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ يﺮﺘﮐﺎﺑ

يﺎﻫ مﺮﮔ ﯽﻔﻨﻣ ياراد ﮏﯾ ﻪﯾﻻ

ﯽﺟرﺎﺧ رد فاﺮﻃا هراﻮﯾد ﯽﻟﻮﻠﺳ دﻮﺧ ﯽﻣ ﺷﺎﺑ ﻨ ﺪ و ﻪﺑ ناﻮﻨﻋ ﮏﯾ

ﺪﺳ ذﻮﻔﻧ ﺎﻧ ﺮﯾﺬﭘ هدﻮﻤﻧ ﻞﻤﻋ و

ﯽﺳﺮﺘﺳد تﺎﺒﯿﮐﺮﺗ ﺰﯾﺮﮕﺑآ ار دوﺪﺤﻣ

ﯽﻣ ﺪﯾﺎﻤﻧ ﻢﮐ ﺖﯿﺳﺎﺴﺣ ياراد اﺬﻟ ﯽﻣ يﺮﺗ

ﺪﺷﺎﺑ 18] . [ رﺪﻨﻠﻫ و

نارﺎﮑﻤﻫ ) 1998 (،

ﻪﮐ ﺪﻧداد شراﺰﮔ ﯽﻟﻮﻨﻓ تﺎﺒﯿﮐﺮﺗ

ﺪﺷر يﺮﺘﮐﺎﺑ -

يﺎﻫ مﺮﮔ ﯽﻔﻨﻣ ار ﺎﺑ ﺐﯾﺮﺨﺗ ﺎﺸﻏ ي ﯽﻧوﺮﯿﺑ

،لﻮﻠﺳ رﺎﻬﻣ ﯽﻣ ﺪﻨﯾﺎﻤﻧ .

ﻠﻋ ناﻮﺘﺑ ﺪﯾﺎﺷ ﻦﯾا ار هﺪﯾﺪﭘ ﻦﯾا ﺖ

ﻪﻧﻮﮔ ﻪﮐ دﻮﻤﻧ ﻪﯿﺟﻮﺗ نزو

ﯽﻟﻮﮑﻟﻮﻣ ﻦﯿﯾﺎﭘ ﺲﻧﺎﺳا ﺎﻫ هزﺎﺟا ﯽﻣ ﺪﻫد ﺎﺗ تﺎﺒﯿﮐﺮﺗ ﻦﯾا ﻪﺑ

يﺎﺸﻏ

ﯽﻠﺧاد يﺮﺘﮐﺎﺑ يﺎﻫ مﺮﮔ ﯽﻔﻨﻣ ذﻮﻔﻧ ﺪﻨﯾﺎﻤﻧ نآ ﺪﺷر زا و ﺎﻫ

ﺪﯾﺎﻤﻧ يﺮﯿﮔﻮﻠﺟ 17]

.[ ياراد ﻪﮐ ﻢﻬﻣ يﺪﯿﺋﻮﻨﭘﺮﺗ ﯽﺗﺎﺒﯿﮐﺮﺗ زا ﯽﮑﯾ

ﺪﺿ ﺮﺛا ﺲﻧﺎﺳا رد و ﺪﺷﺎﺑ ﯽﻣ ﯽﭼرﺎﻗ و ﯽﺑوﺮﮑﯿﻣ ﻧ

ﻮﺑزﺎ ﺰﯿﻧ ﺶﻔﻨﺑ

دﻮﺑ لﻮﻟﺎﻨﯿﻟ ،ﺪﺷ ﺖﻓﺎﯾ .

ﺖﯿﻟﺎﻌﻓ ﺪﺿ ﯽﺑوﺮﮑﯿﻣ ﺮﺘﺸﯿﺑ ﺎﻫﺪﯿﺋﻮﻨﭘﺮﺗ زا

لﻮﻟﺎﻨﯿﻟ ﻪﻠﻤﺟ ﻪﺑ

هوﺮﮔ يﺎﻫ ﺮﮕﻠﻤﻋ طﺎﺒﺗرا دراد .

Table 2 Average diameter (mm) of microbial free zone area of the purple basil essential oil on Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes (well

diffusion agar method).

Purple basil essential oil

Microorganism 1 1.2 1.4 1.8 1.16 1.32 1.64

P. aeruginosa 10.40 ± 0.54^a 9.20±0.28^a 8.00 ±0.52^a 7.10±0.54^a 6.10±0.37^a 00.00±0.17^a 00.00±0.11^a E. coli 11.90±0.52^a 11.10 ±0.50^a 10.00±028^a 8.20±0.56^b 7.30±0.49^b 6.40±0.29^b 00.00±0.10^b S. aureus 13.90 ±0.28^a 12.90 ±0.28^a 11.00 ±0.53^b 9.40 ±0.24^c 8.20 ±0.39^c 7.40 ±0.57^c 6.20±0.27^c S. pyogenes 17.90±0.52^a 16.20±0.53^b 14.00±0.51^c 12.20±0.36^d 10.00±0.48^e 9.10±0.41^e 7.70±0.42^f

Values are means ± standard deviations

ﺑ ﻪ ﺲﻧﺎﺳا ﻪﮐ داد نﺎﺸﻧ ﺞﯾﺎﺘﻧ ﯽﻠﮐ رﻮﻃ ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ ﺮﺘﮐﺎﺑ ﺮﺑ ي يﺎﻫ

ﺖﺒﺜﻣ مﺮﮔ و ﻪﺑ ﻪﺘﺴﺑ نآ ﯽﺸﺨﺑ ﺮﺛا ناﺰﯿﻣ ﺎﻣا ﺖﺳا ﺮﺛﻮﻣ ﯽﻔﻨﻣ مﺮﮔ

ﺖﺳا توﺎﻔﺘﻣ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ عﻮﻧ )

لوﺪﺟ 2 .(

ﻪﺑ طﻮﺑﺮﻣ ﺞﯾﺎﺘﻧ

ﺪﺟ رد رﺎﮔآ رد ﮏﻫﺎﭼ شور لو

2 ﺖﺳا هﺪﺷ هدروآ ، .

ﺞﯾﺎﺘﻧ ﻦﯾا

يﺮﺘﮐﺎﺑ رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻂﺳﻮﺘﻣ ﯽﻠﮐ رﻮﻃ ﻪﺑ ﻪﮐ داد نﺎﺸﻧ -

ﺖﺳا ﺮﺘﺸﯿﺑ ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ .

شور رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻂﺳﻮﺘﻣ

ﺮﺘﮐﺎﺑ ﺮﺑ رﺎﮔآ رد ﮏﻫﺎﭼ ي

ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ 9/

15 ﯽﻠﯿﻣ ﺮﺘﻣ ياﺮﺑ و

يﺮﺘﮐﺎﺑ مﺮﮔ يﺎﻫ ﯽﻔﻨﻣ 15 / 11 ﯽﻠﯿﻣ ﺮﺘﻣ دﻮﺑ . مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻂﺳﻮﺘﻣ

ﺮﺘﮐﺎﺑ ﺮﺑ ﺪﺷر ي

ﯽﺘﻧآ ياﺮﺑ ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ ﻦﯿﺴﯾﺎﻣﻮﮑﻧو ﮏﯿﺗﻮﯿﺑ

8/

11 ﯽﻠﯿﻣ يﺮﺘﮐﺎﺑ ياﺮﺑ و ﺮﺘﻣ ﯽﺘﻧآ ياﺮﺑ ﯽﻔﻨﻣ مﺮﮔ يﺎﻫ

ﮏﯿﺗﻮﯿﺑ

ﻦﯿﺴﯾﺎﻣﺎﺘﻨﺟ 15

ﯽﻠﯿﻣ دﻮﺑ ﺮﺘﻣ .

رﺎﮑﻤﻫ و ﺎﺴﮑﯿﻟ ا

ن ) 2007 (،

ﺲﻧﺎﺳا ﻮﺑزﺎﻧ يﺮﺘﮐﺎﺑ زا يداﺪﻌﺗ ﺮﺑ ار ﺎﻫ

چرﺎﻗ و يرﺎﻤﯿﺑ يﺎﻫ ﯽﺳرﺮﺑ درﻮﻣ ﯽﻫﺎﮕﺸﯾﺎﻣزآ ﻂﯾاﺮﺷ رد از

ﺪﻧدادراﺮﻗ . ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ يﺎﻫ

ﻞﻣﺎﺷ ﻪﻌﻟﺎﻄﻣ ﻦﯾا سﻮﻠﯿﺳﺎﺑ

سﻮﺋﺮﺳ ،ﻼﻧﻮﻤﻟﺎﺳ ، سﻮﺌﺗوﺮﭘ

ﺲﯾرﺎﮕﻟو ﺲﻨﮑﯿﺒﻟآ اﺪﯾﺪﻧﺎﮐ ،

و ...

دﻮﺑ . ﺲﻧﺎﺳا ﻪﮐ داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﻦﯾا ﺞﯾﺎﺘﻧ ﻮﺑزﺎﻧ

ﺪﺷر زا ﯽﺑﻮﺧ ﻪﺑ

ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ يرﺎﻤﯿﺑ يﺎﻫ

يﺮﯿﮔﻮﻠﺟ از دﺮﮐ

19]

،[ ﻦﯾا ﺞﯾﺎﺘﻧ

ناﺮﮕﺸﻫوﮋﭘ

ﻪﺘﻓﺎﯾ ﺎﺑ ﻟﺎﻄﻣ يﺎﻫ ﺖﺷاد ﯽﻧاﻮﺨﻤﻫ ﺮﺿﺎﺣ ﻪﻌ .

ﺞﯾﺎﺘﻧ

ﻪﺑ طﻮﺑﺮﻣ ﻦﯿﯿﻌﺗ

ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﯽﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ

ﺲﻧﺎﺳا ﯽﮔﺪﻨﺸﮐ ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ لوﺪﺟ رد 3

ﺖﺳا هﺪﺷ هدروآ ، .

ﻦﯾا

ﺲﻧﺎﺳا زا ﯽﺘﻈﻠﻏ ﻞﻗاﺪﺣ ﯽﻠﮐ رﻮﻃ ﻪﺑ داد نﺎﺸﻧ ﺞﯾﺎﺘﻧ ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ

ﯽﻣ ﯽﮔﺪﻨﺸﮐ و ﺪﺷر زا ﺖﻌﻧﺎﻤﻣ ﺚﻋﺎﺑ ﻪﮐ يﺮﺘﮐﺎﺑ ياﺮﺑ دﻮﺷ

يﺎﻫ

ﺒﺴﻧ ﺖﺒﺜﻣ مﺮﮔ ﻪﺑ ﺖ

يﺮﺘﮐﺎﺑ يﺎﻫ ﻢﮐ ﯽﻔﻨﻣ مﺮﮔ ﺖﺳا ﺮﺗ

.

يﺎﻤﻧ ﯽﺘﯿﺳﺎﺴﺣ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ

ﺎﻫ رد ﺮﺑاﺮﺑ ﺲﻧﺎﺳا ﻮﺑزﺎﻧ ﺶﻔﻨﺑ

سﺎﺴﺣ ﻪﮐ داد نﺎﺸﻧ ﻦﯾﺮﺗ

موﺎﻘﻣ و ﻦﯾﺮﺗ ﻪﯾﻮﺳ يرﺎﻤﯿﺑ از ﺐﯿﺗﺮﺗ ﻪﺑ

ﺰﻧژﻮﯿﭘ سﻮﮐﻮﮐﻮﺘﭘﺮﺘﺳا و

ازﻮﻨﯾژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ دﻮﺑ

) لواﺪﺟ 2 و 3 .(

ﺲﻧﺎﺳا هﺎﯿﮔ ﻮﺑزﺎﻧ ﻪﺑ رﻮﻃ ﯽﻠﮐ از تﺎﺒﯿﮐﺮﺗ صﺎﺧ ﻞﯿﮑﺸﺗ ﯽﻟﻮﻨﻓ

هﺪﺷ ﺖﺳا . ﻪﻌﻟﺎﻄﻣ رد يدﺮﻤﻧاﻮﺟ ﻂﺳﻮﺗ ﻪﮐ يا و

نارﺎﮑﻤﻫ

) 2002 (،

درﻮﻣ رد تﺎﺒﯿﮐﺮﺗ

ﯽﻟﻮﻨﻓ ، ﯽﮔﮋﯾو يﺎﻫ ﯽﯾﺎﯿﻤﯿﺷ و

يرﺎﺘﺧﺎﺳ 23

هﺪﺷ يروآ ﻊﻤﺟ ﻪﻧﻮﻤﻧ ﻮﺑزﺎﻧ

ﺪﺷ مﺎﺠﻧا ناﺮﯾا رد

ﻪﮐ ﺪﯾدﺮﮔ ﺺﺨﺸﻣ ﻪﻧﻮﮔ

ﯽﯾﺎﻫ زا ﻮﺑزﺎﻧ دراد دﻮﺟو ﻪﮐ

زا ﺮﻈﻧ ناﺰﯿﻣ

،

عﻮﻧ تﺎﺒﯿﮐﺮﺗ ﻟﻮﻨﻓ ﯽ و ﺖﯿﻔﯿﮐ ﺲﻧﺎﺳا رد يزروﺎﺸﮐ ﺖﯿﻤﻫا ياراد

ﯽﻣ ﺪﺷﺎﺑ 20] .[

Table 3 Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the purple basil essential oil on Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and

Streptococcus pyogenes

ﺲﻧﺎﺳا ندﺮﮐ ﻖﯿﻗر ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ ﻒﯿﻌﺿ ﺚﻋﺎﺑ ﺪﺿ ﺮﺛا نﺪﺷ ﺮﺗ

-

،ﺪﺷ نآ ﯽﺑوﺮﮑﯿﻣ يﻮﺤﻧ ﻪﺑ

ﺖﻈﻠﻏ رد ﻪﮐ يﺎﻫ

32 /1 و 64 / 1 ياﺮﺑ

يﺮﺘﮐﺎﺑ ﯽﻔﻨﻣ مﺮﮔ ازﻮﻨﯾژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ

ﺖﻈﻠﻏ رد و 64

/1 ياﺮﺑ يﺮﺘﮐﺎﺑ

ﺎﯿﺷﺮﺷا ﺪﺸﻧ هﺪﻫﺎﺸﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﯽﻠﮐ

. يﺮﺘﮐﺎﺑ درﻮﻣ رد

ﺖﺒﺜﻣ مﺮﮔ سﻮﺋروا سﻮﮐﻮﮐﻮﻠﯿﻓﺎﺘﺳا

ﺰﻧژﻮﯿﭘ سﻮﮐﻮﮐﻮﺘﭘﺮﺘﺳا و رد

ﺖﻈﻠﻏ ﯽﻣﺎﻤﺗ ﺪﺷ هﺪﻫﺎﺸﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺲﻧﺎﺳا يﺎﻫ

) لوﺪﺟ 2 .(

MBC (mg/ml)

MIC (mg/ml)

Microorganism

73.6 36.8

P. aeruginosa

36.8 E. coli 18.4

9.2 4.6

S. aureus

4.6 4.6

S. pyogenes

ﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻪﮐ داد نﺎﺸﻧ ﺞﯾﺎﺘﻧ ﻨ

ﮔﺪﻨ ﺲﻧﺎﺳا ﯽ ﻮﺑزﺎﻧ

ﺶﻔﻨﺑ

يﺮﺘﮐﺎﺑ ﺮﺑ هدوﺪﺤﻣ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ 6/

4 ﺎﺗ 8/

36 ﯽﻠﯿﻣ ﺮﺑ مﺮﮔ

ﯽﻠﯿﻣ دﻮﺑ ﺮﺘﯿﻟ . ﺞﻧر رد ﺰﯿﻧ ﺲﻧﺎﺳا ﯽﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ 6/

4 ﺎﺗ

6/

73 ﯽﻠﯿﻣ ﯽﻠﯿﻣ ﺮﺑ مﺮﮔ دﻮﺑ ﺮﺘﯿﻟ

. ﯽﻣ ﯽﻠﮐ رﻮﻃ ﻪﺑ ﻪﮐ ﺖﻔﮔ ناﻮﺗ

ﺲﻧﺎﺳا ﻮﺑزﺎﻧ يﺮﺘﮐﺎﺑ ﺮﺑ ﺶﻔﻨﺑ ﺖﻈﻠﻏ رد ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ

يﺎﻫ

ﯿﯾﺎﭘ ﻦ ﺮﺛﻮﻣ ﺮﺗ يﺮﺘﮐﺎﺑ زا ﺮﺗ دﻮﺑ ﯽﻔﻨﻣ مﺮﮔ يﺎﻫ

.

4 - ﻪﺠﯿﺘﻧ يﺮﯿﮔ

ﺲﻧﺎﺳا ﻮﺑزﺎﻧ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ ﺪﺷر زا ﯽﺑﻮﺧ ﻪﺑ ﺖﺴﻧاﻮﺗ ﺶﻔﻨﺑ يﺎﻫ

يرﺎﻤﯿﺑ يﺮﯿﮔﻮﻠﺟ از ﺪﻨﮐ

. يﺮﺘﮐﺎﺑ ياﺮﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ يﺎﻫ

ﯽﺘﻧآ زا ﺖﺒﺜﻣ مﺮﮔ دﻮﺑ ﺮﺘﺸﯿﺑ ﻦﯿﺴﯾﺎﻣﻮﮑﻧاو ﮏﯿﺗﻮﯿﺑ

. 28 رد ﺐﯿﮐﺮﺗ

ﺲﻧﺎﺳا ﻮﺑزﺎﻧ ﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺶﻔﻨﺑ زا ﺶﯿﺑ ﻪﮐ

50 ار نآ ﺪﺻرد

ﻞﯿﮑﺸﺗ لﻮﮔوﺮﺘﺳا دﻮﺑ هداد

. ﻖﯿﻗد ﻢﺴﯿﻧﺎﮑﻣ ندﺮﮐ ﺺﺨﺸﻣ ﺖﻬﺟ

ﺲﻧﺎﺳا ﻮﺑزﺎﻧ يﺮﺘﮐﺎﺑ ﺮﺑ ﺶﻔﻨﺑ يرﺎﻤﯿﺑ يﺎﻫ

ﻪﺑ زﺎﯿﻧ از ﺶﻫوﮋﭘ

يﺎﻫ

يﺮﺘﺸﯿﺑ ﯽﻣ ﺪﺷﺎﺑ . ﯽﻣ دﺎﻬﻨﺸﯿﭘ اﺬﻟ ﻂﯾاﺮﺷ رد ﯽﻓﺮﺼﻣ زود ناﺰﯿﻣ دﻮﺷ

نورد دﻮﺷ ﻊﻗاو ﯽﺑﺎﯾزرا درﻮﻣ ﯽﻨﺗ .

5 - ﯽﻧادرﺪﻗ و ﺮﮑﺸﺗ

نﺎﮔﺪﻨﺴﯾﻮﻧ هﺎﮕﺸﻧاد ﯽﺸﻫوﮋﭘ ﺖﻧوﺎﻌﻣ زا ﻪﮐ ﺪﻨﻧاد ﯽﻣ مزﻻ دﻮﺧ ﺮﺑ

ﻪﻨﯾﺰﻫ رﺎﺒﺘﻋا ﻦﯿﻣﺎﺗ ﺖﻬﺟ ﺪﻬﺸﻣ ﯽﺳودﺮﻓ ﯽﻟﺎﻣ يﺎﻫ

، ﻪﻧﺎﻤﯿﻤﺻ

ﺪﻨﯾﺎﻤﻧ ﯽﻧادرﺪﻗ و ﺮﮑﺸﺗ .

6 - ﻊﺑﺎﻨﻣ

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[2] Perez-Perez C, Regalado-Gonzalez C, Rodriguez-Rodriguez CA, Barbosa-Rodríguez J and Villasenor-Ortega F. 2006. Incorporation of antimicrobial agents in food packaging films and coatings. Advances in Agricultural and Food Biotechnology, 11:193-216.

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Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). African Journal of Pharmacy and Pharmacology, 3(2): 58-62.

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[7] Tabatabaei Yazdi F, Alizade Behbahani B, Heidari Sureshjani M. 2014. The comparison of antimicrobial effects of Chevil (Ferulago angulata) extract with a variety of common therapeutic antibiotics in vitro. Arak University of Medical Sciences Journal. 17 (3) :35-46.

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Tarahane'e Nashr publication. Vol 2. Tehran- Iran. 1997, 424 pp.

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Bruchidae). Neotropical Entomology, 42: 89-

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[14] Kolahi Marand S, Tabatabaei Yazdi F, Mortazavi SA, Beig Babaei A. 2016.

Inhibitory and bactericidal effects of artichoke (Cynara scolymus) on pathogenic strains and their comparison with antibiotics in vitro. Qom University Medical Science J,10(2):32-42.

[15] Sadrnia M, Arjomandzadegan M. 2014.

Comparative Study on the Effects of Aloe Vera Extract in Clinical Strains of Staphylococcus Aureus, Klebsiella, Staphylococcus Epidermidis and Escherichia Coli Compared to Antibiotics of Choice. Arak University of Medical Sciences Journal. 17 (6) :39-46.

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Extraction, identification of chemical compounds and antimicrobial activity of purple basil essential oil on food-born pathogenic bacteria

and its comparison with vancomycin and gentamicin antibiotics

Samiei, A. ¹, Tabatabaei Yazdi, F. ^2, Alizadeh Behbahani, B. ³, Mazaheri Tehrani, M.²

1. Ph. D, Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2. Professor, Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3. Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

(Received: 2019/08/10 Accepted:2019/10/12)

The objective of this study was to extract the essential oil of purple basil leaf, to identify its compounds and to investigate its antimicrobial effects on some food-borne pathogenic bacteria through different qualitative and quantitative methods, and eventually, to compare it with some antibiotics including vancomycin and gentamycin in vitro. The essential oil components were identified with GC/MS. The antimicrobial effect of basil essential oil was measured through well diffusion agar (WDA), and finally, the minimum inhibitory concentration (MIC) of the essential oil was determined using microdilution broth and triphenyl tetrazolium chloride. The results revealed that 28 identified compounds constituted 99.28% of the whole essential oil compounds. p-Allylanisole (51.64%) was the most abundant component of the essential oil. In addition, other main components such as n-Tricosane (24.83%) and Linalool (14.81%). In the wall in agar method, the mean free zone diameter was equal to 15.9 mm in the case of Gram-positive bacteria and 11.15 mm in the case of Gram-negative ones. The minimum MIC of purple basil essential oil ranged from 4.6 to 36.8 mg/ml in the case of pathogenic bacteria. Moreover, its minimum bactericidal concentration varied from 4.6 to 73.6. in conclusion, it can be said that purple basil essential oil was effective on Gram-positive bacteria at lower concentrations and could inhibit their growth.

Keywords: Purple basil, Antimicrobial activity, Gentamicin, Vancomycin, Broth microdilution.