ﮔﯿﺎه ﺷﻔﺎﻓﯿﺖ ﻋﺼﺎره رﻧﮓ و ﻋﺼﺎره ﺑﺮ ﻣﯿﺰان ﻣﻘﺪار ﺑﻨﺘﻮﻧﯿﺖ

ﯽﺳرﺮﺑ ﺮﯿﺛﺎﺗ

و ﺖﯿﻧﻮﺘﻨﺑ راﺪﻘﻣ ناﺰﯿﻣ ﺮﺑ هرﺎﺼﻋ pH

و ﮓﻧر هرﺎﺼﻋ ﺖﯿﻓﺎﻔﺷ

هﺎﯿﮔ

ﺎﻧﺎﯾدﻮﺑر ﺎﯾﻮﺘﺳا (Stevia rebaudiana Bertoni)

ﺪﻤﺣا ﷲا حور ﯽﺟرﻮﮔ ي

،

1

ﺪﯿﻤﺣ ﻒﺳﻮﯾ ﯽﻠﻏوا

2

،



ﻒﯾﺮﺷ ﻦﻤﻬﺑ

3

هداز

1 -

،ﺪﺷرا ﯽﺳﺎﻨﺷرﺎﮐ ﻪﺘﺧﻮﻣآ ﺶﻧاد

،ﯽﻧﺎﺒﻏﺎﺑ مﻮﻠﻋ هوﺮﮔ

،يزروﺎﺸﮐ هﺪﮑﺸﻧاد

،نﻼﯿﮔ هﺎﮕﺷاد ﺖﺷر

2 -

،ﯽﻧﺎﺒﻏﺎﺑ مﻮﻠﻋ هوﺮﮔ ،رﺎﯿﺸﻧاد

،يزروﺎﺸﮐ هﺪﮑﺸﻧاد

،نﻼﯿﮔ هﺎﮕﺷاد ﺖﺷر

3 - رﺎﯾدﺎﺘﺳا ،

،نﻼﯿﮔ قﺮﺷ ﯽﺳﺪﻨﻬﻣ و ﯽﻨﻓ هﺪﮑﺸﻧاد ،ﯽﺳﺪﻨﻬﻣ مﻮﻠﻋ و ﺮﺗﻮﯿﭙﻣﺎﮐ ﯽﺳﺪﻨﻬﻣ هوﺮﮔ

،نﻼﯿﮔ هﺎﮕﺸﻧاد ﺮﺳدور

) رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 21 / 06 / 98 رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 23 / 01 / 99 (

ﮑﭼ ﯿ هﺪ

ﯽﺳرﺮﺑ رﻮﻈﻨﻣ ﻪﺑ ﻣ ﺮﯾدﺎﻘﻣ ﺮﺛا

و ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨ ﯿﻧﻮﺘﻨﺑ ﺎﺑ سﺎﻤﺗ مﺎﮕﻨﻫ رد هرﺎﺼﻋpH

ﺖ ناﺰﯿﻣ ﺮﺑ ﯾﺎﻔﺷ هرﺎﺼﻋ ﺖ ﯾﻮﺘﺳا هﺎﯿﮔ ﺎ Stevia) (rebaudiana

Bertoni ﻪﻧﺎﮔاﺪﺟ ترﻮﺻ ﻪﺑ ﻞﻣﺎﻋ ود يور ﯽﺸﻫوﮋﭘ ، ﺐﻟﺎﻗ رد

حﺮﻃ ﻼﻣﺎﮐ ﯽﻓدﺎﺼﺗ ﯾدﺮﮔ اﺮﺟا راﺮﮑﺗ رﺎﻬﭼ رد ﺪ

. ﻞﻣﺎﺷ ،لوا ﻞﻣﺎﻋ ﯿﻧﻮﺘﻨﺑ ﺮﯾدﺎﻘﻣ

رد ﺖ رﺎﻬﭼ

ﺖﻈﻠﻏ ﺎﺑ ﺢﻄﺳ ،يﺎﻫ

0 ) ﺪﻫﺎﺷ (

، 10 ، 20 و 30 ﺮﮔ رد م ﯿﻟ ﺮﺘ ﻞﻣﺎﺷ ،مود ﻞﻣﺎﻋ و مﺎﺧ هرﺎﺼﻋ ﻒﻠﺘﺨﻣ يﺎﻫpH

ﯾدﺎﻘﻣ ﺎﺑ ﺢﻄﺳ ﺞﻨﭘ رد ﺮ

9/

5 ) ﺪﻫﺎﺷ ( ، 5/

5 ، 5 ،

5/

4 و 4 ﺪﻧدﻮﺑ . ﺞﯾﺎﺘﻧ ﻪﺑ ﯾراو ﻪﯾﺰﺠﺗ زا هﺪﻣآ ﺖﺳد هداد ﺲﻧﺎ

داد نﺎﺸﻧ ﺎﻫ ﻪﮐ

ﯿﺴﺑ ﺮﺛا ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ رﺎﻤﯿﺗ ﯽﻨﻌﻣ رﺎ

ﻓ ناﺰﯿﻣ ﺮﺑ يراد لﻮﻨ

، ،ﺪﯿﺋﻮﻨﺗرﺎﮐ

ﯿﻓوﺮﻠﮐ ﻞ ،a ﯿﻓوﺮﻠﮐ ﻞ ﯿﻓوﺮﻠﮐ و b ﻞ و ﻞﮐ ﺪﯿﺋﻮﻧوﻼﻓ هرﺎﺼﻋ ﺎﻫ دراد . ﺘﻤﮐ ﺎﺑ ﺖﯿﻧﻮﺘﻨﺑ زا ﺢﻄﺳ ﻦﯾﺮﺘﻬﺑ ﻦﯿﻨﭽﻤﻫ ،ﯽﯾادﺰﮕﻧر ﻦﯾﺮﺘﺸﯿﺑ و راﺪﻘﻣ ﻦﯾﺮ

راﺪﻘﻣ 20 رد مﺮﮔ

ﺮﺘﯿﻟ ﯾدﺎﻘﻣ ﺎﺑ لﻮﻨﻓ ﺮ 07 /0 ﯽﻠﯿﻣ ﯽﻠﯿﻣ رد مﺮﮔ ﺮﺘﯿﻟ

ﺪﯿﺋﻮﻨﺗرﺎﮐ ، 36

/0 ﯽﻠﯿﻣ ﻞﯿﻓوﺮﻠﮐ ،ﺮﺘﯿﻟ رد مﺮﮔ 1a

ﯽﻠﯿﻣ ،ﺮﺘﯿﻟ رد مﺮﮔ ﻠﮐ

ﻞﯿﻓوﺮ 03b /2 ، ﻞﯿﻓوﺮﻠﮐ ﻞﮐ 04 /3 ﯽﻠﯿﻣ -

ﺪﯿﺋﻮﻧوﻼﻓ راﺪﻘﻣ ﺎﺑ ﺖﯾﺎﻬﻧ رد و ﺮﺘﯿﻟ رد مﺮﮔ 69

/2 وﺮﮑﯿﻣ ﯽﻠﯿﻣ رد مﺮﮔ ﺮﺘﯿﻟ

ﺪﯾدﺮﮔ بﺎﺨﺘﻧا يﺪﻌﺑ ﺶﯾﺎﻣزآ مﺎﺠﻧا ﺖﻬﺟ .

ﯿﻧﻮﺘﻨﺑ دﺮﮑﻠﻤﻋ دﻮﺒﻬﺑ ياﺮﺑ هرﺎﺼﻋ pH

،ﺖ

ﯿﺑ ﺮﮕﻧﺎ ﯽﻨﻌﻣ ﺮﺛا ﺮﺛا و هرﺎﺼﻋ لﻮﻨﻓ ناﺰﯿﻣ ﺮﺑ راد ﯿﺴﺑ

ﯽﻨﻌﻣ رﺎ ﺮﺑ يراد ﻪﯿﻘﺑ ﺮﮐذ تﺎﻔﺻ هﺪﺷ

دﻮﺑ . زا ﺢﻄﺳ ﻦﯾﺮﺘﻬﺑ هرﺎﺼﻋ pH

ﺎﺑ ﻦﯾﺮﺘﺸﯿﺑ ﮓﻧر ﻪﺑ ﻪﺟﻮﺗ و ﯽﯾادز

،ﺮﺸﺑ ﺖﻣﻼﺳ ) pH

5 ( لﻮﻨﻓ ﺮﯾدﺎﻘﻣ ﺎﺑ 15

/0 ﯽﻠﯿﻣ ﯽﻠﯿﻣ رد مﺮﮔ ﺪﯿﺋﻮﻨﺗرﺎﮐ ،ﺮﺘﯿﻟ

42 /1 ﯽﻠﯿﻣ ﻞﯿﻓوﺮﻠﮐ ،ﺮﺘﯿﻟ رد مﺮﮔ 77a

/0 ﯽﻠﯿﻣ ،ﺮﺘﯿﻟ رد مﺮﮔ ﻞﯿﻓوﺮﻠﮐ

84b /0

ﯽﻠﯿﻣ ﺮﺘﯿﻟ رد مﺮﮔ ،

ﻞﯿﻓوﺮﻠﮐ ﻞﮐ 61 /1 ﯽﻠﯿﻣ ﺘﯿﻟ رد مﺮﮔ ﺪﯿﺋﻮﻧوﻼﻓ ﺖﯾﺎﻬﻧ رد و ﺮ راﺪﻘﻣ ﺎﺑ

17 /2 وﺮﮑﯿﻣ ﯽﻠﯿﻣ رد مﺮﮔ ﺮﺘﯿﻟ

ﺪﯾدﺮﮔ بﺎﺨﺘﻧا .

ﻦﯾاﺮﺑﺎﻨﺑ هﺪﻣآ ﺖﺳد ﻪﺑ ﺞﯾﺎﺘﻧ

ﯿﻘﺤﺗ ﻦﯾا زا ﯽﻣ نﺎﺸﻧ ﻖ ﯽﻣ ﻪﮐ ﺪﻫد

رد ﺎﺑ ناﻮﺗ ﺮﻈﻧ ﻦﺘﻓﺮﮔ ﺮﯾدﺎﻘﻣ ﺐﺳﺎﻨﻣ ﮓﻧر تﺎﺒﯿﮐﺮﺗ ﺖﯿﻧﻮﺘﻨﺑ نﻮﭽﻤﻫ يﺮﺑ

) 20 ﺮﺘﯿﻟ رد مﺮﮔ (

ﯾ رد ﮏ ﺐﺳﺎﻨﻣ pH ) 5 ( هرﺎﺼﻋ

،مﺎﺧ ﺖﯿﻓﺎﻔﺷ و ﮓﻧر ﯾﻮﺘﺳا هﺎﯿﮔ هرﺎﺼﻋ

ار ﺎ ﯿﺸﺨﺑ دﻮﺒﻬﺑ ﺪ

.

نﺎﮔژاو ﺪﯿﻠﮐ :

،ﺎﻧﺎﯾدﻮﺑر ﺎﯾﻮﺘﺳا هﺎﯿﮔ هرﺎﺼﻋ ﻨﻓ

ﻮ ﯿﻓوﺮﻠﮐ ،ﺪﯿﺋﻮﻧوﻼﻓ ،ل ﻞ

.

تﺎﺒﺗﺎﮑﻣ لﻮﺌﺴﻣ

:

hamidoghli@guilan.ac.ir

1 - ﻪﻣﺪﻘﻣ

ﻮﺘﺳا ﯾ ﯽﻤﻠﻋ مﺎﻧ ﺎﺑ ﺎ Stevia rebaudiana

ﯿﮔ و ﯽﻔﻠﻋ ﯽﻫﺎ

ﺪﻨﭼ هداﻮﻧﺎﺧ زا ﻪﻟﺎﺳ Asteraceae

ﯿﮔ ﯽﻟﺎﻤﺷ ﻖﻃﺎﻨﻣ ﯽﻣﻮﺑ ﯽﻫﺎ

آ ﯾﺮﻣ

،ﯽﺑﻮﻨﺟ يﺎﮑ ﺑ و ﻪﺋﻮﮔارﺎﭘ

ﯾزﺮ ﯽﻣ ﻞ ﺪﺷﺎﺑ ] 1 .[

ﯿﮔ هﺎ ﯾﻮﺘﺳا ﻢﻫ ﺎ

ﯾ ناﻮﻨﻋ ﻪﺑ ﯿﮔ ﮏﯾ ﻢﻫ و ﯽﯾوراد هﺎﯿﮔ ﮏ

ا هﺎ ظﺎﺤﻟ زا يدﺎﺼﺘﻗ

ﯽﺘﻌﻨﺻ ) ﯾﺮﯿﺷ ﮏﯾ ناﻮﻨﻋ ﻪﺑ ﻦ

ﯿﺒﻃ هﺪﻨﻨﮐ ﯽﻌ ( ﺖﺳا حﺮﻄﻣ .

ﯾﺮﯿﺷ ﻦ

گﺮﺑ ندﻮﺑ ﯾﻮﺘﺳا يﺎﻫ ﻟد ﻪﺑ ﺎ

ﯿ يد رﻮﻀﺣ ﻞ ﻦﭘﺮﺗ

،ﺎﻫ و ﻪﺑ ﯾ ﺘﺳا هﮋ ﯾﻮ لﻮ

ﻠﮔ ﯿ زﻮﮑ ﯾ ﺖﺳا ﺎﻫﺪ ]

2 .[

ﺎﺗ ﺎﯾﻮﺘﺳا يﺎﻫﺪﯾزﻮﮑﯿﻠﮔ 350

ﺮﺑاﺮﺑ

ﺮﯿﺷ ﯾ ﻦ ﺪﻨﺘﺴﻫ زوﺮﮐﻮﺳ زا ﺮﺗ ]

3 .[

ﯾزﻮﮑﯿﻠﮔ ﻦﯿﺑ رد ﺪ

يﺪﻨﻗ يﺎﻫ

ﻒﻠﺘﺨﻣ ﯾزﻮﮑﯿﻠﮔ ،ﺎﯾﻮﺘﺳا رد دﻮﺟﻮﻣ ﺪ

ﯾازﻮﯾدﻮﺒﯾر ،ﺪﯾازﻮﯾﻮﺘﺳا يﺎﻫ ﺪ

و A ياراد C ﯾدﺎﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ ﻨﺘﺴﻫ ﺮ

ﺪ ] 4 .[

يﺪﻨﻗ يﺎﻫﺪﯾزﻮﮑﯿﻠﮔ

ﯾﻮﺘﺳا مﺮﮔ بآ رد ﺎ 80

ﯽﺘﻧﺎﺳ ﻪﺟرد جاﺮﺨﺘﺳا ﻞﺑﺎﻗ ﯽﺘﺣار ﻪﺑ داﺮﮔ

ﺪﻨﺘﺴﻫ . ﻪﺟﻮﺗ ﺎﺑ وﺰﻓازور زوﺮﺑ ﻪﺑ ﯾد نﻮﭼ ﯽﺗﻼﮑﺸﻣ ن

،ﯽﻗﺎﭼ ،ﺖﺑﺎ

ﻪﺘﮑﺳ و ﯽﺒﻠﻗ يﺎﻫ ﯾﻮﺘﺳا يﺬﻐﻣ

ﯽﻣ ﺎ ﯽﺒﺳﺎﻨﻣ رﺎﯿﺴﺑ ﻦﯾﺰﮕﯾﺎﺟ ﺪﻧاﻮﺗ

ﯾﺬﻐﺗ ياﺮﺑ ﺪﺷﺎﺑ ﺪﻨﻗ فﺮﺼﻣ زا ﯽﺷﺎﻧ تﻼﮑﺸﻣ و بﻮﻠﻄﻣﺎﻧ ﻪ ]

5 .[

ﯿﮐﺮﺗ ياراد ﯽﻟﻮﻨﻓ تﺎﺒﯿﮐﺮﺗ و ﺎﻫﺪﯿﺋﻮﻧوﻼﻓ دﻮﺟو ﻞﯿﻟد ﻪﺑ ﺎﯾﻮﺘﺳا تﺎﺒ

ﯽﺘﻧآ ﯾﻻﺎﺑ ﯽﻧاﺪﯿﺴﮐا ﺖﺳا ﯽ

] 6 .[

ﯾا دﻮﺟو ﺎﺑ ،ﻦ

ﯿﻤﻫا يﻻﺎﺑ ﺖ ﺎﯾﻮﺘﺳا

ﯿﻟد ﻪﺑ دﻮﺟو ﻞ ﯿﺒﻃ ﯽﻨﯾﺮﯿﺷ گﺮﺑ رد يﺮﻟﺎﮐ نوﺪﺑ و ﯽﻌ

ﺖﺳا نآ يﺎﻫ

] 7 .[

ﺪﺻ آ رد مدﺮﻣ ﻪﮐ ﺖﺳا لﺎﺳ ﺎﻫ هرﺎﺼﻋ زا ﯽﺑﻮﻨﺟ يﺎﮑﯾﺮﻣ

ﯾﺮﯿﺷ ناﻮﻨﻋ ﻪﺑ ﺎﯾﻮﺘﺳا ﻦ

ﯽﻣ هدﺎﻔﺘﺳا هﺪﻨﻨﮐ ﺪﻨﻨﮐ

. ﯾﺮﯿﺷ صاﻮﺧ و ﯽﻨ

گﺮﺑ ﯽﻧﺎﻣرد ﯾﻮﺘﺳا يﺎﻫ

تاﺮﻈﻧ ،ﺎ ﻪﺑ ار ﯽﻧاواﺮﻓ ﯽﻤﻠﻋ و يدﺎﺼﺘﻗا

ﯾا ﻦ ﯿﮔ ﺖﺳا هدﺮﮐ ﺐﻠﺟ هﺎ ]

8 .[

گﺮﺑ زا ﻞﺻﺎﺣ هرﺎﺼﻋ ﺎﯾﻮﺘﺳا هﺎﯿﮔ ﮏﺸﺧ يﺎﻫ

Stevia)

rebaudiana Bertoni (

ﺑ رﺎﯿﺴﺑ ،لﻮﻠﺤﻣ تﺎﺒﯿﮐﺮﺗ دﻮﺟو ﻞﯿﻟد ﻪ

ﯿﺗ ﯽﻣ هﺮ ﺪﺷﺎﺑ ] 7 [ ﯾﺎﻨﺻ زا يرﺎﯿﺴﺑ ﺎﺗ هﺪﺷ ﺐﺒﺳ ﺮﻣا ﻦﯿﻤﻫ و ﻊ

ﻪﺑ ﺪﻨﻧاﻮﺘﻧ ر ﺪﻨﻨﮐ هدﺎﻔﺘﺳا نآ زا ﯽﺘﺣا .

ﯿﻟد ﻦﯿﻤﻫ ﻪﺑ ﻞ

زا يرﺎﯿﺴﺑ

ﯿﻘﻘﺤﻣ ﻞﺣ هار ﺖﻤﺳ ﻪﺑ ﻦ ﺒﻬﺑ ياﺮﺑ ﯽﯾﺎﻫ

هرﺎﺼﻋ ﺖﯿﻓﺎﻔﺷ و ﮓﻧر دﻮ

ﯿﭘ قﻮﺳ ﺎﯾﻮﺘﺳا زا ﻞﺻﺎﺣ هدﺮﮐ اﺪ

ﯿﮔ ﻦﯾا هرﺎﺼﻋ زا ناﻮﺘﺑ ﺎﺗ ﺪﻧا ﺎﺑ هﺎ

ﺶﺨﺑ رد ،شزرا ﯾوراد يﺎﻫ

ﯽ ﯾاﺬﻏ ﻊﯾﺎﻨﺻ و ﯽ

دﻮﻤﻧ هدﺎﻔﺘﺳا .

رد

ﯿﻘﺤﺗ رد ﺎﺘﺳار ﻦﯾا ور نﻮﮔﺎﻧﻮﮔ تﺎﻘ

ش ﯾﺎﻫ رد ﻪﮐ هﺪﺷ حﺮﻄﻣ ﯽ

لﻼﺣ نﺪﻧﺎﻣ ﯽﻗﺎﺑ ﺐﺒﺳ ﻪﺑ ﺎﻬﻧآ زا يدراﻮﻣ و ﯽﻟآ يﺎﻫ

ﯾ نﻮ يﺎﻫ

ﺼﻣ يﺰﻠﻓ ﺖﺳا هﺪﺷ مﻼﻋا ﺮﻀﻣ نﺎﺴﻧا ﺖﻣﻼﺳ ياﺮﺑ ﺎﻬﻧآ فﺮ ]

9 [ .

ا يﺎﻫﺎﺸﻏ يﺮﯿﮔرﺎﮑﺑ رد و

ﯿﺳاﺮﺘﻠﯿﻓاﺮﺘﻟ يروﺎﻨﻓ و نﻮ

زا ﻪﺘﻓﺮﺸﯿﭘ يﺎﻫ

ﯾﺰﻫ ﺎﺑ يداﻮﻣ ﯽﻣ هدﺎﻔﺘﺳا ﻻﺎﺑ ﻪﻨ

ﻪﺑ هزاﺪﻧا نﺎﻤﻫ ﻪﺑ و دﻮﺷ يژﻮﻟﻮﻨﮑﺗ

يﺎﻫ ﯾﺰﻫﺮﭘ ﻪﻨ ﯿﻧ ﺪﻧراد زﺎ ]

10 .[

زا هدﺎﻔﺘﺳا شور

و ﺎﻫ

ﯿﮐﺮﺗ ﯽﺗﺎﺒ ﺎﺑ ﯾﺰﻫ ﻢﮐ يژﻮﻟﻮﻨﮑﺗ د ﺎﺑ ﻪﻨ

نﺎﺴﻧا ﯽﺘﻣﻼﺳ ﻦﺘﻓﺮﮔ ﺮﻈﻧ ر

ﺖﺳا هﺪﺷ ﻊﻗاو ﻪﺟﻮﺗ درﻮﻣ .

بﺬﺟ ﯿﺗ ﮓﻧر بذﺎﺟ ﻂﺳﻮﺗ هﺮ ﺎﻫ

ﯾاﺮﻓ ﺪﻨ ﯽﺷزرا ﺎﺑ لﻼﺣ ندﺮﮐ ﻪﻓﺎﺿا نوﺪﺑ ﻪﮐ ﺖﺳا

ﯽﻤﺳ يﺎﻫ

ﯽﻣ ﯾ ﺖﺳد فﺎﻔﺷ هرﺎﺼﻋ ﮏﯾ ﺪﯿﻟﻮﺗ ﻪﺑ ناﻮﺗ ﺖﻓﺎ

] 7 .[

ﯾ ناﻮﻨﻋ ﻪﺑ ﺖﯿﻧﻮﺘﻨﺑ ﮏ

ﯾﺰﻫ ﻢﮐ و يﻮﻗ ﯽﺑذﺎﺟ ،ﯽﻧﺪﻌﻣ سر كﺎﺧ ﻪﻨ

ﯽﻣ ﯿﺴﺑ رد ﻪﮐ ﺪﺷﺎﺑ يرﺎ

ﺖﺳا دﻮﺟﻮﻣ نﺎﻬﺟ طﺎﻘﻧ زا ]

11 .[

ﺶﯿﭘ

ﯿﻟد ﻪﺑ بآ ﺎﺑ ﺖﯿﻧﻮﺘﻨﺑ رﺎﻤﯿﺗ نﺪﺷ مرﻮﺘﻣ ﻞ

تارذ ﺐﺒﺳ ﺖﯿﻧﻮﺘﻨﺑ

ﯿﻓﺮﻇ ﺶﯾاﺰﻓا ﯽﻣ بﺬﺟ ﺖ

دﻮﺷ ] 12 .[

زا ﯿﻘﺤﺗ رد ﺖﯿﻧﻮﺘﻨﺑ تﺎﻘ

ﮓﻧر ﺖﻬﺟ رد ﯽﻧﻮﮔﺎﻧﻮﮔ ﺖﺳا هﺪﺷ هدﺎﻔﺘﺳا هرﺎﺼﻋ يﺮﺑ

. رد

ﯿﻘﺤﺗ ﺠﻧا ﺎﻣﺮﺧ ﺖﺑﺮﺷ يور ﻪﮐ ﯽﻘ زا هدﺎﻔﺘﺳا ﺎﺑ ﺪﺷ مﺎ

25 رد مﺮﮔ

زا ﺮﺘﯿﻟ لﻮﻠﺤﻣ 20 ﯿﺗﻻژ هاﺮﻤﻫ ﻪﺑ ﺖﯿﻧﻮﺘﻨﺑ يﺪﺻرد ،هرﺎﺼﻋ رد ﻦ

ﯿﺛﺎﺗ ﺮ 40 ﺪﺷ هﺪﻫﺎﺸﻣ تروﺪﮐ ﺶﻫﺎﮐ رد يﺪﺻرد ]

13 .[

ﻦﯿﻨﭽﻤﻫ

ﯿﻧ ﺮﮑﺸﯿﻧ هرﺎﺼﻋ يزﺎﺳ فﺎﻔﺷ ﺖﻬﺟ رد ﺖﯿﻧﻮﺘﻨﺑ زا هﺪﺷ هدﺎﻔﺘﺳا ﺰ

ﺖﺳا ] 14 .[

ﯽﻣ ﺮﺘﻣارﺎﭘ ﯽﺳرﺮﺑ ﺎﺑ ناﻮﺗ ﯿﺛﺎﺗ يﺎﻫ

ﺮ ﺪﻨﻧﺎﻣ يراﺬﮔ pH

ﺎﻣ ﯽﯾارﺎﮐ ،ﺖﯿﻧﻮﺘﻨﺑ ﺐﺳﺎﻨﻣ راﺪﻘﻣ ﺎﺑ هرﺎﺼﻋ ﯾاﺰﻓا ار ﺮﻈﻧ درﻮﻣ هد

ﺶ

داد . ﯿﻬﺑ رﻮﻈﻨﻣ ﻪﺑ ﻪﻨ

ﺖﯿﻧﻮﺘﻨﺑ ﺎﺑ سﺎﻤﺗ رد ﺪﯿﺳا ﺖﻈﻠﻏ يزﺎﺳ )

ﺖﻬﺟ

لﺎﻌﻓ ﺖﯿﻧﻮﺘﻨﺑ يزﺎﺳ (

ﯿﻓﺎﻔﺷ ﺐﺒﺳ ﻪﺘﯾﺪﯿﺳا ناﺰﯿﻣ ، ﺪﺷ اﺰﻠﮐ ﻦﻏور ﺖ

] 15 .[

يﺮﺒﮕﻧر رد ﺪﯿﺳا ﺎﺑ هﺪﺷ لﺎﻌﻓ ﺖﯿﻧﻮﺘﻨﺑ زا هدﺎﻔﺘﺳا ﻦﯿﻨﭽﻤﻫ ﯿﺴﺑ ﻢﻟﺎﭘ ﻦﻏور دﻮﺑ ﺮﺛﻮﻣ رﺎ

] 16 .[

ﯿﻘﺤﺗ ﺞﯾﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻧا تﺎﻘ

مﺎﺠ

،هﺪﺷ ﯾا رد ﻦ ﺮﯿﺛﺎﺗ ،هﺪﺷ لﺎﻌﻓ ﺖﯿﻧﻮﺘﻨﺑ زا هدﺎﻔﺘﺳا ﺮﺑ هوﻼﻋ ﺶﻫوﮋﭘ

ﯿﻣ ناﺰ ﯽﺳرﺮﺑ درﻮﻣ ﺖﯿﻧﻮﺘﻨﺑ دﺮﮑﻠﻤﻋ ﺶﯾاﺰﻓا رد ﺰﯿﻧ هرﺎﺼﻋ pH

ﺖﻓﺮﮔ راﺮﻗ .

ﯾا ﻦﯾاﺮﺑﺎﻨﺑ ﺶﻫوﮋﭘ ﻦ هﺮﻬﺑ ﺎﺑ

ﯾاﺮﻓ زا يﺮﯿﮔ زا ﺪﻌﺑ يﺎﻫﺪﻨ

هرﺎﺼﻋ ﯿﮔ ﻪﺑ ،يﺮ فﺎﻔﺷ رﻮﻈﻨﻣ ﺳا هرﺎﺼﻋ يزﺎﺳ

ﺎﯾﻮﺘ Stevia)

rebaudiana Bertoni (

رد نآ زا هدﺎﻔﺘﺳا ﯽﯾﺎﻬﻧ فﺪﻫ ﺎﺑ

ﯾاﺬﻏ و ﯽﯾوراد ﻒﻠﺘﺨﻣ ﻊﯾﺎﻨﺻ ﺖﺳا هﺪﺷ مﺎﺠﻧا ﯽ

.

2 - شور و داﻮﻣ ﺎﻫ

ﯾا ﻦ رد ﺶﻫوﮋﭘ ﯿﯾﺎﭘ

لﺎﺳ ﺰ 1397 د ﯾﺎﻣزآ ر يﺰﮐﺮﻣ و ﯽﻧﺎﺒﻏﺎﺑ هﺎﮕﺸ

ﯾدﺮﮔ مﺎﺠﻧا نﻼﯿﮔ هﺎﮕﺸﻧاد ﺪ

. ﯿﺗ ﻞﯾاوا رد گﺮﺑ هﺎﻣﺮ

هزﺎﺗ يﺎﻫ هﺎﯿﮔ

ﯾﻮﺘﺳا ﺎ (Stevia rebaudiana Bertoni) ﻪﻋرﺰﻣ زا

و يا رد ﻊﻗا

نﺎﺘﺳا ﯿﻬﺗ نﻼﯿﮔ ﺪﺷ ﻪ . ﻪﺴﯿﮐ نورد ندﺮﮐ ﮏﺸﺧ زا ﺲﭘ و

ﯾﺎﻣزآ مﺎﺠﻧا ﺖﻬﺟ ﯽﮑﯿﺘﺳﻼﭘ ﺖﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ تﺎﺸ

.

ﺢﻄﺳ ﻦﯾﺮﺘﻬﺑ ﻦﯿﯿﻌﺗ ياﺮﺑ ﯿﻧﻮﺘﻨﺑ

ﺖ

، ﺢﻄﺳ رﺎﻬﭼ 0

، 10 ، 20 ، 30

رد مﺮﮔ ﺖﻓﺮﮔ راﺮﻗ ﺶﯾﺎﻣزآ درﻮﻣ ﺮﺘﯿﻟ .

ﻞﮑﺷ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ )

1 ( ﺮﻫ زا

ﻮﻠﺤﻣ هﺪﺷ لﺎﻌﻓ ﺖﯿﻧﻮﺘﻨﺑ ﺮﯾدﺎﻘﻣ زا ﺢﻄﺳ ل

20 ﯾدﺮﮔ ﻪﯿﻬﺗ ﺪﺻرد ﺪ

ﺪﻨﭼ ياﺮﺑ و ﺖﯾﺎﻬﻧرد و ﺪﺷ هداد ﺖﺣاﺮﺘﺳا ﺖﻋﺎﺳ

ﻪﺑ 1 ﯿﻟ ﺮﺘ

ﺼﻋ تﺪﻣ ﻪﺑ و ﺪﺷ ﻪﻓﺎﺿا مﺎﺧ هرﺎ 20

ﯿﻃﺎﻨﻐﻣ نﺰﻤﻫ يور ﻪﻘﯿﻗد ﯽﺴ

يﺎﻣد رد 25 ﯽﺘﻧﺎﺳ ﻪﺟرد ﺪﯾدﺮﮔ طﻮﻠﺨﻣ داﺮﮔ

ﺬﻏﺎﮐ زا ﺖﯾﺎﻬﻧ رد و

هرﺎﻤﺷ ﻦﻤﺗاو ﯽﻓﺎﺻ 42

ﺪﺷ هداد رﻮﺒﻋ ]

17 [.

ياﺮﺑ ﺪﻌﺑ ﻪﻠﺣﺮﻣ رد ﯿﻣ ﻦﯿﯿﻌﺗ

ﻣ ناﺰ ﺐﺳﺎﻨ زا هدﺎﻔﺘﺳا ﺎﺑ مﺎﺧ هرﺎﺼﻋ ،pH

ﺖﻈﻠﻏ ﺎﺑ HCl

6 ﺮﺘﻣ چا ﯽﭘ و ﺪﺻرد (Ultrabasic UB-10)

ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻪﺑ

pH ﻞﻣﺎﺷ 4 ، 5/

4 ، 5 ، 5/

5 ، 9/

5 ) ﺪﻫﺎﺷ ( ﺪﺷ هﺪﻧﺎﺳر ]

10 [

، ﺲﭙﺳ

ﯿﻧﻮﺘﻨﺑ ﻪﻨﯿﻬﺑ ﺢﻄﺳ ﺎﺣ ﺖ

ﻞﺒﻗ ﻪﻠﺣﺮﻣ زا ﻞﺻ )

20 ﺮﺘﯿﻟ رد مﺮﮔ (

نآ ﻪﺑ

ﯾدﺮﮔ ﻪﻓﺎﺿا ﺪ

. نﺪﺷ طﻮﻠﺨﻣ زا ﺲﭘ ﺖﯾﺎﻬﻧرد رد ﺐﯿﮐﺮﺗ

يﺎﻣد 25

ﯽﺘﻧﺎﺳ ﻪﺟرد نﺎﻣز و داﺮﮔ

20 ﺪﺷ هداد رﻮﺒﻋ ﯽﻓﺎﺻ ﺬﻏﺎﮐ زا ﻪﻘﯿﻗد .

Fig 1 Overview of pre and post extraction steps to increase the clarification of Stevia extract

2 - 1 - ﯽﮑﯾژﻮﻟﻮﯾﺰﯿﻓ تﺎﻔﺻ يﺮﯿﮔ هزاﺪﻧا

2 - 1 - 1 - هزاﺪﻧا ﻞﮐ لﻮﻨﻓ يﺮﯿﮔ

هزاﺪﻧا ﻞﮐ لﻮﻨﻓ ناﺰﯿﻣ يﺮﯿﮔ حﻼﺻا ﻮﺘﻟﺎﮐ ﻮﯿﺳ ﻦﯿﻟﻮﻓ شور ﻂﺳﻮﺗ

ﺪﯾدﺮﮔ مﺎﺠﻧا هﺪﺷ .

شور ﻦﯾا رد 2/

0 ﺎﺑ ﺺﻟﺎﺧ هرﺎﺼﻋ ﺮﺘﯿﻟ ﯽﻠﯿﻣ 1

ﻦﯿﻟﻮﻓ فﺮﻌﻣ ﺮﺘﯿﻟ ﯽﻠﯿﻣ 10

ﺪﺷ طﻮﻠﺨﻣ ﺪﺻرد .

ﺪﻌﺑ ﻪﻠﺣﺮﻣ رد 8/

0

ﯽﻠﯿﻣ تﺎﻨﺑﺮﮐ ﻢﯾﺪﺳ ﺮﺘﯿﻟ 5/

7 ﺪﯾدﺮﮔ ﻪﻓﺎﺿا نآ ﻪﺑ ﺪﺻرد )

ﻞﮑﺷ 2 -

9 ( ياﺮﺑ ﻪﻧﻮﻤﻧ ﺖﯾﺎﻬﻧرد ، 10

يﺎﻣد رد ﻪﻘﯿﻗد 50

ﯽﺘﻧﺎﺳ ﻪﺟرد ﮔ

داﺮ

رد بﺬﺟ قﺎﺗا يﺎﻣد رد نﺪﺷ دﺮﺳ زا ﺲﭘ و ﺖﻓﺮﮔ راﺮﻗ 750

ﺮﺘﻣﻮﺘﻓﻮﺘﮑﭙﺳا هﺎﮕﺘﺳد ﻂﺳﻮﺗ ﺮﺘﻣﻮﻧﺎﻧ )

لﺪﻣ T80- UV/VIS

Spectrometer +

ﺖﮐﺮﺷ ﺖﺧﺎﺳ نﺎﺘﺴﻠﮕﻧا PG

( ﺪﺷ هﺪﻧاﻮﺧ .

ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ياﺮﺑ 2/

0 ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟ ﯽﻠﯿﻣ ]

18 [.

2 - 1 - 2 - هزاﺪﻧا يﺮﯿﮔ ﻞﯿﻓوﺮﻠﮐ ،A

و ﻞﮐ ﻞﯿﻓوﺮﻠﮐ ،B

ﺪﯿﺋﻮﻨﺗرﺎﮐ

ﻣ ﯿﻓوﺮﻠﮐ ناﺰﯿ ﻞ

ﻪﻟدﺎﻌﻣ A ) 1 ( و ﻪﻟدﺎﻌﻣ B ) 2 ( ﯿﺋﻮﻨﺗرﺎﮐ و ﻪﻟدﺎﻌﻣ ﺪ

) 3 ( شور ﻂﺳﻮﺗ نارﺎﮑﻤﻫ و بﺮﻋ ﻮﺑا

ﺪﺷ مﺎﺠﻧا ]

19 [.

ﯾا رد ﻦ

شور 5 ﻣ ﯿ ﻟ ﯽﻠ ﯿ ﻮﺘﺳا هرﺎﺼﻋ ﺮﺘ ﯾ

ﺎﺑ ﺎ 30 ﻣ ﯿ ﯽﻠ ﻟﯿ نﻮﺘﺳا ﺮﺘ 85 ﺪﺻرد

رد ﯾ ﺗ ﻪﻈﻔﺤﻣ ﮏ ﯿ

تﺪﻣ ﻪﺑ و هﺪﺷ طﻮﻠﺨﻣ هﺮ 15

يﺎﻣد رد ﺖﻋﺎﺳ

ﭙﺳ و هﺪﻧﺎﻣ ﯽﻗﺎﺑ قﺎﺗا نورد و رﻮﺒﻋ ﯽﻓﺎﺻ ﺬﻏﺎﮐ زا ﺲ

ﯾ ﮏﺳﻼﻓ ﮏ

ﯽﻤﺠﺣ ﯿﺳو ﻪﺑ نﻮﺘﺳا ﻪﻠ 85 ﻢﺠﺣ ﻪﺑ ﺪﺻرد 100

ﯿﻟ ﯽﻠﯿﻣ هﺪﻧﺎﺳر ﺮﺘ

ﺪﺷ . ﻣﯿ ا بﺬﺟ ناﺰ ﯾ

رد طﻮﻠﺨﻣ ﻦ 440

، 644 و 662 ﺎﺑ ﺮﺘﻣﻮﻧﺎﻧ

ﺮﺘﻣﻮﺘﻓﻮﺘﮑﭙﺳا زا هدﺎﻔﺘﺳا )

لﺪﻣ T80- UV/VIS

Spectrometer +

ﺖﮐﺮﺷ ﺖﺧﺎﺳ نﺎﺘﺴﻠﮕﻧا PG

( هزاﺪﻧا ﮔﯿ يﺮ

ﯾدﺮﮔ ﺪ . نﻮﺘﺳا 85 ﺪﺻرد ﯾدﺮﮔ بﺎﺨﺘﻧا ﮏﻨﻠﺑ ناﻮﻨﻋ ﻪﺑ ﺪ

.

ﻮﺘﺤﻣ ﯾ ﺋﻮﻨﺗورﺎﮐ تﺎ ﯿ

ﻓوﺮﻠﮐ و ﺎﻫﺪ ﯿ

ﻞ تﻻدﺎﻌﻣ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ

) 1 ، 2 و 3 ( ﺪﯾدﺮﮔ ﻪﺒﺳﺎﺤﻣ .

(1) Chlorophyll A (mg/L) = (9.784× Abs 662) - (0.99 × Abs 644)

(2) Chlorophyll B (mg/L) = (21.426× Abs 644) - (4.65 × Abs 662)

(3) Total carotenoids (mg/L) = (4.695× Abs 440) – 0.369 (chl. A + chl. B)

Equations, 1 Chlorophyll A, 2 Chlorophyll B, 3 Total carotenoids

2 - 1 - 3 - هزاﺪﻧا يﺮﯿﮔ ﺪﯿﺋﻮﻧوﻼﻓ

ﯿﺋﻮﻧوﻼﻓ ﻂﺳﻮﺗ هداد حﺮﺷ شور ﻂﺳﻮﺗ ﻞﮐ ﺪ نارﺎﮑﻤﻫ و ﻮﺘﻧاود

هزاﺪﻧا ﺪﯾدﺮﮔ يﺮﯿﮔ ]

20 [.

ﯾا رد شور ﻦ 25 /0 ﯿﻣ ﯽﻠ ﻟ ﺎﺑ هرﺎﺼﻋ ﺮﺘﯿ

25 /1 ﯿﻟ ﯽﻠﯿﻣ ﻪﻟﻮﻟرد ﺮﻄﻘﻣ بآ ﺮﺘ ﯾدﺮﮔ طﻮﻠﺨﻣ ﺶﯾﺎﻣزآ

زا ﺲﭘ ﺪ

ﻓﺎﺿا ﻪ ندﺮﮐ 75 ﯾﺪﺳ ﺖﯾﺮﺘﯿﻧ لﻮﻠﺤﻣ ﺮﺘﯿﻟوﺮﮑﯿﻣ ﻢ

5 ﺪﺻرد رﻻﻮﻣ

زا ﺪﻌﺑ 6 ﻪﻘﯿﻗد 150 ﯿﻨﯿﻤﻟآ ﺪﯾﺮﻠﮐ ﺮﺘﯿﻟوﺮﮑﯿﻣ مﻮ

10 رﻻﻮﻣ ﺪﺻرد

زا ﺪﻌﺑ و ﻪﻓﺎﺿا 5

ﻪﻘﯿﻗد 500 ﯿﺴﮐورﺪﯿﻫ ﺮﺘﯿﻟوﺮﮑﯿﻣ ﺳ ﺪ

ﯾﺪ ﻢ 1 ﻻﻮﻣ ر

ﻪﻓﺎﺿا تﺎﺒﯿﮐﺮﺗ ﻪﺑ ﺪﯾدﺮﮔ

. ﯾﺎﻬﻧ رد و ﻢﺠﺣ ﻪﺑ ﺮﻄﻘﻣ بآ ﻂﺳﻮﺗ ﺖ

5/

2 ﯿﻟ ﯽﻠﯿﻣ ﺎﺳر ﺮﺘ ﻮﺘﮑﭙﺳا هﺎﮕﺘﺳد ﻂﺳﻮﺗ بﺬﺟ و ﺪﺷ هﺪﻧ ﺮﺘﻣﻮﺘﻓ

) لﺪﻣ T80- UV/VIS Spectrometer +

ﺖﮐﺮﺷ ﺖﺧﺎﺳ PG

نﺎﺘﺴﻠﮕﻧا ( جﻮﻣ لﻮﻃ رد 510

ﺪﺷ هﺪﻧاﻮﺧ ﺮﺘﻣﻮﻧﺎﻧ .

2 - 1 - 4 - ﺖﯿﻓﺎﻔﺷ ﺪﺻرد

ﯿﻓﺎﻔﺷ ﺪﺻرد ناﺰﯿﻣ يرﻮﻧ بﺬﺟ نﺪﻧاﻮﺧ ﺎﺑ ﺖ

670 ﺮﺘﻣﻮﻧﺎﻧ و ﻞﺒﻗ

ﺑ ﯿﮔ هزاﺪﻧا ﺖﻬﺟ ﻪﯿﻔﺼﺗ تﺎﯿﻠﻤﻋ زا ﺪﻌ هﺎﮕﺘﺳد ﻂﺳﻮﺗ تروﺪﮐ يﺮ

ﺮﺘﻣﻮﺘﻓﻮﺘﮑﭙﺳا )

لﺪﻣ +T80- UV/VIS Spectrometer

ﺖﮐﺮﺷ ﺖﺧﺎﺳ نﺎﺘﺴﻠﮕﻧا PG

( و رد بﺬﺟ ناﺰﯿﻣ نداد راﺮﻗ ﻪﻟدﺎﻌﻣ

) 4 ( ﯾدﺮﮔ ﻪﺒﺳﺎﺤﻣ ﺪ

] 21 [.

% clarification=

Equation 4 Percent of extract clarification

يرﺎﻣآ تﺎﺒﺳﺎﺤﻣ - 2 - 2

ﻪﺑ و راﺮﮑﺗ رﺎﻬﭼ رد ﯽﻓدﺎﺼﺗ ﻼﻣﺎﮐ ﻪﯾﺎﭘ حﺮﻃ ترﻮﺻ ﻪﺑ ﺶﯾﺎﻣزآ ﺪﯾدﺮﮔ رﻮﻈﻨﻣ ﻞﻣﺎﻋ ﺮﻫ ياﺮﺑ ﻪﻧﺎﮔاﺪﺟ ترﻮﺻ )

لوﺪﺟ 1 .(

ﻪﯾﺰﺠﺗ

ﺲﻧﺎﯾراو هداد ﺎﻫ ﺎﺑ هدﺎﻔﺘﺳا زا مﺮﻧ راﺰﻓا (Version 9) SAS

درﻮﻣ ﯾﺰﺠﺗ ﻪ و ﯿﻠﺤﺗ ﻞ راﺮﻗ ﺖﻓﺮﮔ . ﻪﺟﻮﺗ ﺎﺑ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﺖﻬﺟ

ﻪﺑ ﻞﻣﺎﻋ ﺮﻫ ياﺮﺑ ﻒﻠﺘﺨﻣ حﻮﻄﺳ يﺎﻫرﺎﻤﯿﺗ ﻦﺘﺷاد )

و ﺖﯿﻧﻮﺘﻨﺑ

ﯽﮐﻮﺗ نﻮﻣزآ زا (pH )

ﯽﻨﻌﻣ فﻼﺘﺧا دﺎﻤﺘﻋا ﻞﺑﺎﻗ راد

( ﺢﻄﺳ رد

لﺎﻤﺘﺣا ﯾ ﮏ ﺪﯾدﺮﮔ هدﺎﻔﺘﺳا ﺪﺻرد و

و لواﺪﺟ ﻢﺳر رادﻮﻤﻧ

ﺎﻫ ﯿﻧ ﺰ

هدﺎﻔﺘﺳا ﺎﺑ زا مﺮﻧ راﺰﻓا Excel ﯿﺳﺮﺗ ﻢ دﺮﮔ . ﯾﺪ

of Stevia extract clarification

ents to improve Different levels of experimental treatm

1 Table

Experimental treatments

pH of crowed extracts Bentonite concentration (g/l) 4

4.5 5 5.5 5.9^*

0^* 10 20 30

4 4 Replication

*: Control

3 - ﯾﺎﺘﻧ ﺚﺤﺑ و ﺞ

3 - 1 - ﻨﻓ ﻮ ل ﻞﮐ

ﺞﯾﺎﺘﻧ ﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﻦ

ﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ ﻞﮐ لﻮﻨﻓ ﺺﺧﺎﺷ ﺮﺑ ﺖ

لوﺪﺟ رد )

4 (

، توﺎﻔﺗ رﺎﯿﺴﺑ ﯽﻨﻌﻣ ﺎﺑ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ حﻮﻄﺳ راد

ار ﺪﻫﺎﺷ ﯽﻣ نﺎﺸﻧ ﺪﻫد . ﺖﯿﻧﻮﺘﻨﺑ زا هدﺎﻔﺘﺳا ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻪﺘﺒﻟا

) 10 ﺎﺗ 30 ﺮﺘﯿﻟ رد مﺮﮔ (

فﻼﺘﺧا ﻢﻫ ﺎﺑ ﻞﮐ لﻮﻨﻓ ناﺰﯿﻣ ﺶﻫﺎﮐ رد

رﺎﯿﺴﺑ ﯽﻨﻌﻣ يراد ﺪﻨﺘﺷاﺪﻧ ﻢﻫﺎﺑ .

ﯾﺎﺘﻧ ﺞ ﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ حﻮﻄﺳ ﻦ

ﻒﻠﺘﺨﻣ pH لوﺪﺟ ) 5 (

، ﻦﯾﺮﺘﻤﮐ ﯿﻣ ناﺰ ﻨﻓ ﻮ ل ﻞﮐ طﻮﺑﺮﻣ ﻪﺑ

زا هدﺎﻔﺘﺳا ﺢﻄﺳ رد pH

5/

4 و 5 ﯽﻣ نﺎﺸﻧ ار ﺪﻫد

. ﻨﻓ راﺪﻘﻣ ﻮ ل

ﻞﮐ ﯿﺗ رد رﺎﻤ يﺎﻫ زا هدﺎﻔﺘﺳا ﺢﻄﺳ رد pH

5/

4 و 5 ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ

15 /0 ﯿﻣ ﯽﻠ د مﺮﮔ ﯿﻣ ر ﯽﻠ ﺪﻫﺎﺷ رﺎﻤﯿﺗ رد و ﺮﺘﯿﻟ 9/

5 ﺎﺑ 17 /0

ﯿﻣ ﯽﻠ د مﺮﮔ ﯿﻣ ر ﯽﻠ ﯿﻟ ﯽﻣ هﺪﻫﺎﺸﻣ ﺮﺘ ددﺮﮔ

. بﺬﺟ ﻨﻓ ﻮ ل ﺎﻫ ﻂﺳﻮﺗ

ﯿﻧﻮﺘﻨﺑ ﻟد ﻪﺑ ًﻻﺎﻤﺘﺣا ﺖ ﯿ

ﻞﺤﻣ ندﻮﺑ سﺮﺘﺳد رد ﻞ لﺎﻌﻓ بﺬﺟ يﺎﻫ

ﯿﻧﻮﺘﻨﺑ يور ﺮﺑ ﺖﺳا ﺖ

] 22 .[

شراﺰﮔ سﺎﺳا ﺮﺑ يﺎﻫ

نﺎﺳﺎﻧاﺪﻣﺎﺴﮐﻻ

نارﺎﮑﻤﻫ و )

2012 ( ﻖﻨﯿﺳ و كﺎﯾﺎﻧ و )

2007 ( ﯿﻣ ﻨﻓ بﺬﺟ ناﺰ ﻮ

ل

ﯿﻧﻮﺘﻨﺑ ﻂﺳﻮﺗ اﺰﻓا ﺎﺑ ﺖ

ﯾ راﺪﻘﻣ ﺶ لﻮﻠﺤﻣ pH

ﺶﻫﺎﮐ ﻞﯿﻟد ﻪﺑ

ﯿﺳاﺰﯿﻧﻮﯾ ﺶﻫﺎﮐ نﻮ ﯽﻣ

ﯾ ﺪﺑﺎ ] 14 ] [ 23 .[

3 - 2 - ﯿﺋﻮﻨﺗرﺎﮐ ﺪ

ﺞﯾﺎﺘﻧ ﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﻦ

ﺮﯿﺛﺎﺗ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ ﯿﻧﻮﺘﻨﺑ

ﺖ راﺪﻘﻣ ﺮﺑ

ﯿﺋﻮﻨﺗرﺎﮐ ﺪ لوﺪﺟ رد )

4 (

، توﺎﻔﺗ رﺎﯿﺴﺑ ﯽﻨﻌﻣ ﺢﻄﺳ ﺎﺗ يراد 10

ﯽﻤﻧ نﺎﺸﻧ ﺪﻫﺎﺷ ﺎﺑ ﺮﺘﯿﻟ رد مﺮﮔ ﺢﻄﺳ زا ﺎﻣا ﺪﻫد

20 ﺎﻣ ﺮﺘﯿﻟ رد مﺮﮔ

ﺶﻫﺎﮐ رﺎﯿﺴﺑ ﯽﻨﻌﻣ ﯽﻣ هﺪﻫﺎﺸﻣ ار ﺪﯿﺋﻮﻨﺗرﺎﮐ راد ﻢﯿﻨﮐ

. ﺢﻄﺳ 20 مﺮﮔ

رد ﯿﻟ ﺮﺘ هﺪﻨﻫد نﺎﺸﻧ ﯾﺮﺘﻤﮐ

راﺪﻘﻣ ﻦ ﯿﺋﻮﻨﺗرﺎﮐ ﺪ ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 36 /0

ﯿﻣ ﯽﻠ مﺮﮔ رد ﯿﻟ ﺮﺘ

،ﻞﺑﺎﻘﻣ رد ﯾﺮﺘﺸﯿﺑ

راﺪﻘﻣ ﻦ ﯿﺋﻮﻨﺗرﺎﮐ ﻣ ﺪ ﻪﺑ طﻮﺑﺮ

ﯿﺗ رﺎﻤ ﺪﻫﺎﺷ ) 0 مﺮﮔ رد ﯿﻟ ﺮﺘ ( ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 73 /0 ﯿﻣ ﯽﻠ مﺮﮔ رد ﯿﻟ ﺮﺘ

ﺖﺳا . ﺞﯾﺎﺘﻧ ﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﻦ

ﯿﺋﻮﻨﺗرﺎﮐ راﺪﻘﻣ ﺮﺑ pH ﺪ

لوﺪﺟ ) 5 ( ﯽﻣ نﺎﺸﻧ

،ﺪﻫد رد ﺢﻄﺳ 5/

4 ﯾﺮﺘﻤﮐ راﺪﻘﻣ ﻦ ﯿﺋﻮﻨﺗرﺎﮐ ﺪ ﻦﯿﮕﻧﺎﯿﻣ ﺎﺑ

83 /0 ﯿﻣ ﯽﻠ مﺮﮔ رد ﯿﻟ ﺮﺘ و ﯾﺮﺘﺸﯿﺑ راﺪﻘﻣ ﻦ ﯿﺋﻮﻨﺗرﺎﮐ ﻣ ﺪ ﯿﺗ ﻪﺑ طﻮﺑﺮ رﺎﻤ

4 ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 61 /1 ﯿﻣ ﯽﻠ رد مﺮﮔ ﯿﻟ ﺮﺘ ﺖﺳا . بﺬﺟ ﻢﺴﯿﻧﺎﮑﻣ زﻮﻨﻫ

ﯿﻧ ﺺﺨﺸﻣ ﺖﯿﻧﻮﺘﻨﺑ يور ﺪﯿﺋﻮﻨﺗورﺎﮐ و ﻦﺗورﺎﮐ ﺖﺴ

] 24 .[

ﺎﻣا ﯽﻣ -

ﯿﯿﻐﺗ ﺎﺑ ناﻮﺗ رد ﯽﺗاﺮ

ﺖﻬﺟ هرﺎﺼﻋ pH ءﺎﻘﺗرا

ﺪﯿﺋﻮﻨﺗرﺎﮐ فﺬﺣ رد

ﺖﺴﺟ هﺮﻬﺑ .

ﯾا ﺎﺑ رﺎﮐ ﻦ ﯿﻧﻮﺘﻨﺑ ﺢﻄﺳ رد ﺖﺒﺜﻣ رﺎﺑ دﺎﺠﯾا ﺖ

] 25 [ و

ﺖﯿﺒﻄﻗ ﯿﭘ دﺎﯾز يﺮﯾﺬﭘ ﻪﻧﺎﮔود يﺎﻫﺪﻧﻮ

] 26 [ بﺬﺟ ﺐﺒﺳ

ﯿﺋﻮﻨﺗرﺎﮐ ﺪ ﯽﻣ ﺎﻫ دﻮﺷ .

Table 2 Results of analysis of variance charactrestics studied in Stevia rebaudiana Bertoni

MS

DF sov Phenol Cartenoeids

Chlorophyll a Chlorophyll

b Total

chlorophyll Flavenoeids

Clarification

Bentonite(T) 3

0.00**

0.14**

28.49**

3.64**

40.11**

1.25**

128.48*

Error 12

0.00 0.00

0.03 0.05

0.07 0.02

14.69

C.V (%) -

11.39 5.75

7.50 9.08

5.42 5.45

5.04

** ,*: Significantly difference at 1% and 5% probability levels, respectively.

Table 3 Results of analysis of variance charactrestics studied in Stevia rebaudiana Bertoni

sov MS

DF Phenol Cartenoeids

Chlorophyll a Chlorophyll

b Total

chlorophyll Flavenoeids

Clarification

pH 4

0.00*

0.33**

0.95**

1.55**

4.92**

0.17**

41.81 ^ns

Error 15

0.00 0.00

0.02 0.01

0.04 0.03

45.64

C.V (%) -

5.11 6.17

11.30 7.81

7.03 8.05

8.55

** , ,* ns: Significantly difference at 1% and 5% probability levels and non-significant, respectively.

3 - 3 - ﯿﻓوﺮﻠﮐ ﻞ a

ﺞﯾﺎﺘﻧ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﺮﯿﺛﺎﺗ

راﺪﻘﻣ ﺮﺑ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ

ﯿﻓوﺮﻠﮐ ﻞ لوﺪﺟ a ) 4 ( نﺎﺸﻧ فﻼﺘﺧا هﺪﻨﻫد رﺎﯿﺴﺑ

ﯽﻨﻌﻣ راد ﻦﯿﺑ

ﺖﺳا هﺪﺷ هدﺮﺑ رﺎﮐ ﻪﺑ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ حﻮﻄﺳ و ﺪﻫﺎﺷ .

ﻪﺘﺒﻟا

توﺎﻔﺗ رﺎﯿﺴﺑ ﯽﻨﻌﻣ يراد ﺶﻫﺎﮐ رد ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻦﯿﺑ

ﻞﯿﻓوﺮﻠﮐ ناﺰﯿﻣ ﯽﻤﻧ هﺪﻫﺎﺸﻣ a

دﻮﺷ . ﯾﺎﺘﻧ ﻦﯿﻨﭽﻤﻫ ﺞ

ﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﻦ

ﻒﻠﺘﺨﻣ حﻮﻄﺳ لوﺪﺟ pH

) 5 ( داد نﺎﺸﻧ ﻪﮐ ناﺰﯿﻣ ﻦﯾﺮﺘﻤﮐ

ﯿﻓوﺮﻠﮐ ﻞ زا هدﺎﻔﺘﺳا ﻪﺑ طﻮﺑﺮﻣ a ﺢﻄﺳ رد pH

5 ﯽﻣ ﺪﺷﺎﺑ . راﺪﻘﻣ

ﮐ ﯿﻓوﺮﻠ ﻞ ﯿﺗ رد a زا هدﺎﻔﺘﺳا رﺎﻤ ﺢﻄﺳ رد pH

5 ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 77 /0 ﯿﻣ

ﯽﻠ رد مﺮﮔ ﯾﺮﺘﺸﯿﺑ و ﺮﺘﯿﻟ نآ ناﺰﯿﻣ ﻦ

د ﺪﻫﺎﺷ رﺎﻤﯿﺗ ر )

9/

5 ( ﺎﺑ

ﻦﯿﮕﻧﺎﯿﻣ 05 /2 ﯿﻣ ﯽﻠ رد مﺮﮔ ﯿﻟ ﺪﯾدﺮﮔ هﺪﻫﺎﺸﻣ ﺮﺘ .

ﺎﺑ هرﺎﺼﻋ رﺎﻤﯿﺗ

ﯿﻓوﺮﻠﮐ بﺬﺟ رد ﯽﯾﻻﺎﺑ ﺖﯿﻓﺮﻇ ﺖﯿﻧﻮﺘﻨﺑ دراد ﻞ

] 27 .[

ﯽﺸﻫوﮋﭘ رد

ﻪﮐ نارﺎﮑﻤﻫ و ﺰﯿﻟاﺰﻧﻮﮔ ﻂﺳﻮﺗ )

1994 ( ﻞﯿﻓوﺮﻠﮐ بﺬﺟ يور ﺮﺑ a

ﺖﯿﻧﻮﺘﻨﺑ ﻂﺳﻮﺗ نﻮﺘﺳا لﻮﻠﺤﻣ زا ﺪﯿﺳا ﺎﺑ هﺪﺷ رﺎﻤﯿﺗ

ﺪﯾدﺮﮔ مﺎﺠﻧا

ﻪﮐ داد نﺎﺸﻧ ﺎﺑ هرﺎﺼﻋ رﺎﻤﯿﺗ

ﺖﯿﻧﻮﺘﻨﺑ ﺪﯿﺳا ﺎﺑ هﺪﺷ لﺎﻌﻓ ﯿﻟد ﻪﺑ

ﻞ

نوﺰﻓازور ﻞﯾﺎﻤﺗ ﺳ رد لﺎﻌﻓ طﺎﻘﻧ

ﺶﻫﺎﮐ رد ﯽﺘﺒﺜﻣ ﺮﺛا بذﺎﺟ ﺢﻄ

ﯿﻓوﺮﻠﮐ ﻞ دراد a داد ﻪﺋارا ار ﯽﻬﺑﺎﺸﻣ ﺞﯾﺎﺘﻧ و ]

28 .[

3 - 4 - ﯿﻓوﺮﻠﮐ ﻞ b

ﺞﯾﺎﺘﻧ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﺮﯿﺛﺎﺗ

ﺺﺧﺎﺷ ﺮﺑ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ

ﯿﻓوﺮﻠﮐ ﻞ لوﺪﺟb ) 4 ( ، فﻼﺘﺧا رﺎﯿﺴﺑ ﯽﻨﻌﻣ يراد ار ﺢﻄﺳ ﺎﺗ 10

ﺪﻫﺎﺷ ﺎﺑ ﺮﺘﯿﻟ رد مﺮﮔ نﺎﺸﻧ

ﻧ ﯽﻤ ﺪﻫد

، ﺢﻄﺳ زا 20 ﻦﯾا ﺮﺘﯿﻟ رد مﺮﮔ

ﺖﺳا دﻮﻬﺸﻣ ﺪﻫﺎﺷ ﺎﺑ فﻼﺘﺧا .

ﺞﯾﺎﺘﻧ لوﺪﺟ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ

ﻒﻠﺘﺨﻣ حﻮﻄﺳ لوﺪﺟ pH

) 5 ( نﺎﺸﻧ ﯽﻣ ﺪﻫد ﻪﮐ ﻦﯾﺮﺘﻤﮐ راﺪﻘﻣ

ﮐ ﯿﻓوﺮﻠ ﻞ ﯿﺗ ردb زا هدﺎﻔﺘﺳا رﺎﻤ ﺢﻄﺳ رد pH

5 ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 84 /0

ﯿﻣ ﯽﻠ رد مﺮﮔ و ﺮﺘﯿﻟ ﺘﺸﯿﺑ ناﺰﯿﻣ ﻦﯾﺮ ﺪﻫﺎﺷ رﺎﻤﯿﺗ رد

) 9/

5 ( ﺎﺑ ﻦﯿﮕﻧﺎﯿﻣ

16 /2 ﯿﻣ ﯽﻠ رد مﺮﮔ ﯿﻟ ﺮﺘ ﺖﺳا . ﯿﻧﻮﺘﻨﺑ زا ﻊﯾﺎﻨﺻ رد ﺖ

لﺎﻌﻓ يﺎﻫ

ﯿﻓوﺮﻠﮐ بﺬﺟ ﺖﻬﺟ ﻞ

ﯽﻣ هدﺎﻔﺘﺳا ﻦﻏور زا ﺎﻫ دﻮﺷ

] 15 [ . ﺗﯿ رﺎﻤ

ﺎﺑ ﺖﯿﻧﻮﺘﻨﺑ ﺳا

ﯿﺪ ﺗﺎﮐ يﻮﺸﺘﺴﺷ ﺮﺑ هوﻼﻋ ﯿ

نﻮ قرو زا ﺎﻫ يﺎﻫ

ﺖﺸﻫ

ﯽﺼﻟﺎﺧﺎﻧ ،ﯽﻌﻠﺿ ﺎﻫ

ﯾ ﺴﻠﮐ ﺪﻨﻧﺎﻣ ﯽ ﯿ

ﯽﻣ ﻞﺣ ار ﺖ ﮐ و ﺪﻨﮐ

ﺗﺎﯿ نﻮ يﺎﻫ

ﻮﻌﺗ ﻞﺑﺎﻗ ﯾ ﺎﺑ ار ﺾ ﯾ نﻮ ﻫ يﺎﻫ ﯿ ﺎﺟ نژورﺪ ﯾ

ﺰﮕ ﯾ ﯽﻣ ﻦ ﺪﻨﮐ يﻮﺳ زا و

ﻪﯾﻻ نﺪﺷ ﻞﺨﻠﺨﺘﻣ ﺐﺒﺳ ﺮﮕﯾد ﯽﻣ ﺖﯿﻧﻮﺘﻨﺑ يﺎﻫ

ددﺮﮔ ﻪﮐ ا ﻪﻤﻫ ﯾ ﻦ

ﻐﺗ ﯿ تاﺮ ﺶﯾاﺰﻓا ﺐﺒﺳ ﺬﻓﺎﻨﻣ ﺮﻄﻗ و ﺢﻄﺳ

ﯽﻣ ﺖﯿﻧﻮﺘﻨﺑ تارذ ددﺮﮔ

] 29 .[

3 - 5 - ﯿﻓوﺮﻠﮐ ﻞ ﻞﮐ

ﺞﯾﺎﺘﻧ راﺪﻘﻣ ﺺﺧﺎﺷ ﺮﺑ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ

ﯿﻓوﺮﻠﮐ ﻞ ﻞﮐ لوﺪﺟ ) 4 ( ﯽﻨﻌﻣ رﺎﯿﺴﺑ فﻼﺘﺧا حﻮﻄﺳ ﻦﯿﺑ راد

ار ﺪﻫﺎﺷ ﺎﺑ ﺖﯿﻧﻮﺘﻨﺑ دﺮﺑرﺎﮐ ﻒﻠﺘﺨﻣ ﯽﻣ نﺎﺸﻧ

ﺪﻫد . زا 10 ﺎﺗ 20 مﺮﮔ

ﯽﻣ هﺪﻫﺎﺸﻣ ار ﻞﮐ ﻞﯿﻓوﺮﻠﮐ ﺶﻫﺎﮐ ﺎﻣ ﺎﻣا ﻢﯿﻨﮐ

هدﺎﻔﺘﺳا راﺪﻘﻣ 30

ﺮﺘﯿﻟ رد مﺮﮔ ﺖﯿﻧﻮﺘﻨﺑ

فﻼﺘﺧا رﺎﯿﺴﺑ ﯽﻨﻌﻣ راﺪﻘﻣ ﺎﺑ يراد 20

رد مﺮﮔ

ﯽﻤﻧ نﺎﺸﻧ ﺮﺘﯿﻟ ﺪﻫد

. ﺘﻧ ﯾﺎ ﺞ ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ pH

لوﺪﺟ ) 5 ( ﻪﮐ داد نﺎﺸﻧ ﻦﯾﺮﺘﻤﮐ

ﮐ راﺪﻘﻣ ﯿﻓوﺮﻠ ﻞ ﻞﮐ ﯿﺗ رد رﺎﻤ

زا هدﺎﻔﺘﺳا ﺢﻄﺳ رد pH

5 ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 61 /1 ﯿﻣ ﯽﻠ رد مﺮﮔ رد و ﺮﺘﯿﻟ

ﺪﻫﺎﺷ رﺎﻤﯿﺗ )

9/

5 ( ﺎﺑ راﺪﻘﻣ 22 /4 ﯿﻣ ﯽﻠ رد مﺮﮔ ﯿﻟ ﺮﺘ ﺖﺳا . رﺎﻤﯿﺗ

اد ﻞﯿﻓوﺮﻠﮐ بﺬﺟ رد ﯽﯾﻻﺎﺑ ﺖﯿﻓﺮﻇ ﺖﯿﻧﻮﺘﻨﺑ ﺎﺑ هرﺎﺼﻋ

،در رد

ﻪﮐ ﯽﺸﻫوﮋﭘ نارﺎﮑﻤﻫ و ﻦﭼ ﻂﺳﻮﺗ

) 2012 ( ﺖﻓﺎﺑ هرﺎﺼﻋ يور ﺮﺑ

ﺪﯾدﺮﮔ مﺎﺠﻧا ﺞﻧﺮﺑ ار ﯽﻬﺑﺎﺸﻣ ﺞﯾﺎﺘﻧ

ﻪﮐ داد نﺎﺸﻧ راﺪﻘﻣ رد ﺶﯾاﺰﻓا

ﺎﺑ هرﺎﺼﻋ رد ﺖﯿﻧﻮﺘﻨﺑ زا هدﺎﻔﺘﺳا ناﺰﯿﻣ ﺶﻫﺎﮐ ﺐﺒﺳ ﺞﻧﺮﺑ ﺖﻓ

ﻞﯿﻓوﺮﻠﮐ ﯽﻣ ددﺮﮔ ﺖﯾﺎﺳ و ﺢﻄﺳ ﺶﯾاﺰﻓا نآ ﻞﯿﻟد ﻪﮐ لﺎﻌﻓ يﺎﻫ

ﯽﻣ بﺬﺟ ﺪﺷﺎﺑ ] 27 .[

ﯽﻣ ﺮﻈﻧ ﻪﺑ ﻞﮐ ﻞﯿﻓوﺮﻠﮐ بﺬﺟ ﺶﻫﺎﮐ ﺪﺳر

ﻞﯿﻟد ود ﻪﺑ ﺖﯿﻧﻮﺘﻨﺑ راﺪﻘﻣ ﺶﯾاﺰﻓا ﺎﺑ ﺪﺷﺎﺑ

. ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ﺎﺑ ًﻻوا

ﺶﻫﺎﮐ بذﺎﺟ ﺢﻄﺳ ﻢﻫ ﻪﺑ ﺖﯿﻧﻮﺘﻨﺑ تارذ لﺎﺼﺗا ﻞﯿﻟد ﻪﺑ ﺖﯿﻧﻮﺘﻨﺑ ﯽﻣ ﻞﯿﻓوﺮﻠﮐ زا ت ﯽﻤﺠﺣ رد ﺖﯿﻧﻮﺘﻨﺑ ﺖﻈﻠﻏ ﺶﯾاﺰﻓا ﺎﯿﻧﺎﺛ و ﺪﺑﺎﯾ

نﺎﮑﻣ ﺶﯾاﺰﻓا ﺐﺒﺳ ﻞﮐ ﺮﯿﻏ لﺎﻌﻓ يﺎﻫ

ﯽﻣ بذﺎﺟ يور عﺎﺒﺷا دﻮﺷ

] 30 .[

ﯽﺸﻫوﮋﭘ رد ﺮﮕﯾد

ﻪﮐ نارﺎﮑﻤﻫ و يرﺎﭼآ ﻂﺳﻮﺗ )

2016 ( ﺮﺑ

ﮓﻧر يور ﺪﯾدﺮﮔ مﺎﺠﻧا ﻦﻏور ﯽﯾادز

شراﺰﮔ ار ﯽﻬﺑﺎﺸﻣ ﺞﯾﺎﺘﻧ

ﺶﻫوﮋﭘ ﻦﯾا رد ﺪﻧدﻮﻤﻧ ﺖﯿﻧﻮﺘﻨﺑ

هﺪﺷ رﺎﻤﯿﺗ يﺎﻫ ﺐﺒﺳ ﺪﯿﺳا ﺎﺑ

ﺶﻫﺎﮐ ﻞﮐ ﻞﯿﻓوﺮﻠﮐ ناﺰﯿﻣ ﺎﺑ رﺎﻤﯿﺗ نوﺪﺑ ﺖﯿﻧﻮﺘﻨﺑ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد

ﺪﯾدﺮﮔ ﺪﯿﺳا ]

31 .[

ﻪﮐ ﻦﯾا ﻞﯿﻟد ﻪﺑ ﻟﺎﻌﻓ

ﯿ ﻓ ﺖ ﯿﺰ ﯾ ﺷ و ﯽﮑ ﯿﻤ ﯿﺎﯾ ﯽ

و ﻪﺑ سر كﺎﺧ ﺢﻄﺳ رد دﻮﺟﻮﻣ ﯾ

ﺳا زا هدﺎﻔﺘﺳا ﺎﺑ هﮋ ﯿ

اﺰﻓا ﺪ ﯾ ﺶ

ﯽﻣ ﯾ ﺪﺑﺎ ] 32 ] [ 33 .[

3 - 6 - ﯿﺋﻮﻧوﻼﻓ ﺪ

ﺞﯾﺎﺘﻧ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﺮﯿﺛﺎﺗ

ﺺﺧﺎﺷ ﺮﺑ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ

ﯿﺋﻮﻧوﻼﻓ راﺪﻘﻣ ﺪ

لوﺪﺟ ) 4 (

، فﻼﺘﺧا رﺎﯿﺴﺑ ﯽﻨﻌﻣ ﺢﻄﺳ ﺎﺗ ار يراد

10 ﺪﻫﺎﺷ ﺎﺑ ﺮﺘﯿﻟ رد مﺮﮔ ﻧ

نﺎﺸ ﻧ ﯽﻤ ﺪﻫد راﺪﻘﻣ رد ﺶﯾاﺰﻓا ﯽﻟو

زا ﺖﯿﻧﻮﺘﻨﺑ 20

ﯽﻨﻌﻣ رﺎﯿﺴﺑ ﺶﻫﺎﮐ ﺐﺒﺳ ﺮﺘﯿﻟ رد مﺮﮔ ناﺰﯿﻣ راد

ﯽﻣ ﺪﯿﺋﻮﻧوﻼﻓ ددﺮﮔ

. ﯾﺎﺘﻧ ﺞ ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ pH

لوﺪﺟ ) 5 ( راﺪﻘﻣ ﻦﯾﺮﺘﻤﮐ ﻪﮐ داد نﺎﺸﻧ ﯿﺋﻮﻧوﻼﻓ

ﺪ ﯿﺗ رد هدﺎﻔﺘﺳا رﺎﻤ

زا ﺢﻄﺳ رد pH 5

ﯿﮕﻧﺎﯿﻣ ﺎﺑ ﻦ 17 /2 مﺮﮔوﺮﮑﯿﻣ رد

ﯿﻣ ﯽﻠ ﺮﺘﯿﻟ و

ﺢﻄﺳ رﺎﻤﯿﺗ رد راﺪﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ 4

ﺎﺑ 61 /2 ﯿﻣ وﺮﮑ مﺮﮔ رد ﯿﻣ ﯽﻠ ﯿﻟ ﺮﺘ

ﯽﻣ ﺪﺷﺎﺑ . نارﺎﮑﻤﻫ و ﻦﻤﮐﻮﮔ )

2001 ( درﻮﻣرد ار ﯽﻬﺑﺎﺸﻣ ﺞﯾﺎﺘﻧ

ﺶﻫﺎﮐ راﺪﻘﻣ ﯿﺋﻮﻧوﻼﻓ ﺪ ﻨﻋ ﻪﺑ ﺎﻫ هرﺎﺼﻋ تروﺪﮐ ﻞﻣاﻮﻋ زا ﯽﮑﯾ ناﻮ

ﺖﯿﻧﻮﺘﻨﺑ ﺎﺑ رﺎﻤﯿﺗ زا ﺪﻌﺑ ﺐﯿﺳ بآ رد ﺪﻧدﻮﻤﻧ شراﺰﮔ

] 34 [ . ﺎﺑ

ﯿﻧﻮﺘﻨﺑ راﺪﻘﻣ ﺶﯾاﺰﻓا رد ﺖ

ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ نآ بﺬﺟ راﺪﻘﻣ ﻪﻨﯿﻬﺑ pH

ﯾاﺰﻓا ﯾ ﺶﯾاﺰﻓا بذﺎﺟ ﺢﻄﺳ ﺶ ﺖﺳا ﻪﺘﻓﺎ

. ﻧﻮﺘﻨﺑ ﯿ ﺖ ﯿﺗ ﺎﺑ هﺪﺸﻧ رﺎﻤ

ﺪﯿﺳا ياراد ﯾ ﯽﻟﻮﻤﻌﻣ كزﺎﻧ قرو ﮏ و

ﺗﯿ ﻪﻐ دﻮﺧ ﺢﻄﺳ رد يا ﺖﺳا

زا ﺲﭘ ﯽﻟو ﯿﺗ

ﺎﺑ رﺎﻤ ﺳا ﯿ ﻧﻮﺘﻨﺑ ﺢﻄﺳ ،ﺪ ﯿ

ﯽﻣ ﺮﺗ ﻞﺨﻠﺨﺘﻣ و ﯽﮐﺮﮐ ﺖ -

،دﻮﺷ ﻪﮐ ﺐﺒﺳ اﺰﻓا ﯾ ﺶ ﯽﻣ بذﺎﺟ ﺢﻄﺳ ددﺮﮔ

] 16 .[

Table 4 Mean comparison effect of different concentrations of bentonite on the quantitative properties of Stevia extract

Flavenoeids (μg/ml)

Total Chlorophyll

(mg/l)

Chlorophyll b (mg/l)

Chlorophyll a (mg/l)

Carotenoids

(mg/l)

Phenol (mg/ml)

Bentonite

(g/l) 3.34±0.08^a

3.17±0.10^a 2.69±0.13^b 2.10±0.24^c

9.53±0.33^a 4.51±0.30^b 3.04±0.14^c 2.66±0.23^c

3.74±0.24^a 3.10±0.20^a 2.03±0.03^b 1.67±0.35^b

6.45±0.25^a 1.40±0.10^b 1±0.11^b 0.98±0.21^b

0.73±0.01^a 0.66±0.04^a 0.36±0.03^b 0.38±0.01^b

0.17±0.01^a 0.05±0.01^b

0.07±0.0^b

0.08±0.0^b

0 10 20 30 Comparison of the average effect of different amounts of bentonite on total phenol content. There is no significant

difference between 1% or 5% probability level using Tukey test. The top lines of the graph represent the Means ± SD.

Table 5 Mean comparison effect of different pH of extract on quantitative traits of Stevia extract Flavenoeids

(μg/ml)

Total Chlorophyll

(mg/l)

Chlorophyll b (mg/l)

Chlorophyll a (mg/l)

Carotenoids

(mg/l)

Phenol (mg/ml)

pH extract

2.61±0.14^a

2.34±0.07^ab 2.17±0.17^b 2.20±0.31^ab

2.59±0.16 ^ab

1.99±0.25^c 3.73±0.31^a 1.61±0.14^c 2.89±0.10^b 4.22±0.09^a

0.97±0.11^bc 2.13±0.34^a 0.84±0.08^c 1.44±0.06^b 2.16±0.12^a

1.01±0.14^cd 1.37±0.22^bc 0.77±0.17^d 1.44±0.07^b 2.05±0.06^a

1.61±0.06^a 0.83±0.04^c 1.42±0.07^ab

1.38±0.07^b 1.32±0.12^b

0.16±0.00^ab 0.15±0.01^b 0.15±0.00^b 0.16±0.00^ab

0.17±0.00^a

4 4.5

5 5.5 5.9 Comparison of the average effect of different amounts of bentonite on total phenol content. There is no significant

difference between 1% or 5% probability level using Tukey test. The top lines of the graph represent the Means ± SD.

3 - 7 - ﯿﻓﺎﻔﺷ ﺪﺻرد ﺖ

ﯾﺎﺘﻧ ﺞ لوﺪﺟ ) 2 ( ﯽﻨﻌﻣ توﺎﻔﺗ ﺖﯿﻧﻮﺘﻨﺑ ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻦﯿﺑ ار يراد

ﻢﻫ ﺎﺑ ار ﯽﻣ نﺎﺸﻧ ﺪﻫد . ﻞﮑﺷ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ )

2 ( ناﺰﯿﻣ ﺶﯾاﺰﻓا

ﺎﺗ ﺖﯿﻧﻮﺘﻨﺑ 20

ﺖﯿﻓﺎﻔﺷ رد ﻪﺟﻮﺗ ﻞﺑﺎﻗ دﻮﺒﻬﺑ ﺪﻫﺎﺷ ﺎﻣ ﺮﺘﯿﻟ رد مﺮﮔ

ﻢﯿﺘﺴﻫ هرﺎﺼﻋ .

ر داﻮﻣ دﺮﺑرﺎﮐ ﮓﻧ

ﯿﻧﻮﺘﻨﺑ ﺪﻨﻧﺎﻤﻫ يﺮﺑ ﯽﻣ ﺖ

رد ﺪﻧاﻮﺗ

ﯿﻓﺎﻔﺷ ﺖ ﺎﺗ هرﺎﺼﻋ 79

ﻮﺷ ﻊﻗاو ﺮﺛﻮﻣ ﺪﺻرد د

ﯿﯿﻐﺗ ﺎﺑ ﻪﮐ تاﺮ

ﺮﺘﻣارﺎﭘ ﯿﺛﺎﺗ يﺎﻫ ﺮ ﺪﻨﻧﺎﻣ يراﺬﮔ ﯽﻣ pH

ﺎﺗ ار ﺖﯿﻓﺎﻔﺷ ناﺰﯿﻣ ناﻮﺗ 82

داد ﺶﯾاﺰﻓا ﺪﺻرد .

راﺪﻘﻣ ﺖﯿﻧﻮﺘﻨﺑ دﺮﺑرﺎﮐ ﻒﻠﺘﺨﻣ ﺮﯾدﺎﻘﻣ ﻦﯿﺑ رد

20 هرﺎﺼﻋ ﺖﯿﻓﺎﻔﺷ ﺮﯿﺛﺎﺗ ناﺰﯿﻣ ﺎﺑ هرﺎﺼﻋ ﺮﺘﯿﻟ رد مﺮﮔ 94

/ 79

دﻮﺑ راﺪﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ ياراد ﺪﺻرد .

ﺖﯿﻓﺎﻔﺷ ناﺰﯿﻣ ﻦﯾﺮﺘﻤﮐ ﻦﯿﻨﭽﻤﻫ

ﻋ سﺎﻤﺗ ﻪﺑ طﻮﺑﺮﻣ ﺰﯿﻧ هرﺎﺼ 10

رد مﺮﮔ ناﺰﯿﻣ ﺎﺑ هرﺎﺼﻋ ﺮﺘﯿﻟ

ﺖﯿﻓﺎﻔﺷ 55 / 69 دﻮﺑ ﺪﺻرد .

زا ﺶﯿﺑ ﺶﯾاﺰﻓا ﻪﺘﺒﻟا 20

ناﺰﯿﻣ رد مﺮﮔ

ﺖﺳا ﻪﺘﺷاد هرﺎﺼﻋ ﺖﯿﻓﺎﻔﺷ يور ﯽﻔﻨﻣ ﺮﯿﺛﺎﺗ ﺖﯿﻧﻮﺘﻨﺑ .

ﯿﺑ رد ﻦ

ﻒﻠﺘﺨﻣ حﻮﻄﺳ ﻦﯾﺮﺘﺸﯿﺑ ﺰﯿﻧ pH

ﻪﺑ طﻮﺑﺮﻣ ﺖﯿﻓﺎﻔﺷ رد ﺮﯿﺛﺎﺗ راﺪﻘﻣ

ﻪﺘﯾﺪﯿﺳا 4 و 5 ﯿﻣ ﻪﺑ ﺐﯿﺗﺮﺗ ﻪﺑ ﻪﮐ ناﺰ

55 / 82 و 43 / 82 دﻮﺑ ﺪﺻرد .

ﯿﻓﺎﻔﺷ رد ﺮﯿﺛﺎﺗ ناﺰﯿﻣ ﻦﯾﺮﺘﻤﮐ و ﻣ ﺖ

ﯾﺪﯿﺳا ﻪﺑ طﻮﺑﺮ ﻪﺘ

5/

4 و 9/

5

) ﺪﻫﺎﺷ ( ﯾدﺎﻘﻣ ﺎﺑ ﺐﯿﺗﺮﺗ ﻪﺑ ﻪﮐ ﺮ

01 / 76 و 68 / 76 دﻮﺑ ﺪﺻرد .

و ﻦﭼ نارﺎﮑﻤﻫ

) 2001 ( درﻮﻣرد ار ﯽﻬﺑﺎﺸﻣ ﺞﯾﺎﺘﻧ ﻓﺎﻔﺷ ﺶﯾاﺰﻓا

ﯿ ﺖ

هرﺎﺼﻋ هرﺎﺼﻋرد ﺗ يﺎﻫ ﺪﻧداد شراﺰﮔ ﺖﯿﻧﻮﺘﻨﺑ ﺎﺑ هﺪﺷ رﺎﻤﯿ ]

27 [.

نارﺎﮑﻤﻫ و نﺎﺳﺎﻧاﺪﻣﺎﺴﮐﻻ )

2012 ( درﻮﻣرد ار ﯽﻬﺑﺎﺸﻣ ﺞﯾﺎﺘﻧ ﺰﯿﻧ

ﺪﻧدﻮﻤﻧ شراﺰﮔ ﺖﯿﻧﻮﺘﻨﺑ راﺪﻘﻣ ﺶﯾاﺰﻓا ﺎﺑ ﺖﯿﻓﺎﻔﺷ ناﺰﯿﻣ ﺶﻫﺎﮐ ] 14 .[

Fig 2 Effect of different amounts of bentonite on the clarification of Stevia rebaudiana extract

4 - ﯿﮔ ﻪﺠﯿﺘﻧ ﯽﻠﮐ يﺮ

ﻪﺑ داد نﺎﺸﻧ ﺶﻫوﮋﭘ ﻦﯾا ﺞﯾﺎﺘﻧ ﯽﻠﮐ رﻮﻃ رﺎﻤﯿﺗ ﻪﮐ

20 رد مﺮﮔ ﯿﻟ ﺮﺘ

ﺖﯿﻧﻮﺘﻨﺑ رد هرﺎﺼﻋ pH 5 ﺐﺒﺳ دﻮﺒﻬﺑ ﯿﻓﺎﻔﺷ رد هرﺎﺼﻋ ﺖ

ﺎﯾﻮﺘﺳا (Stevia rebaudiana Bertoni) ﺎﺗ

82 ﯽﻣ ﺪﺻرد -

ددﺮﮔ . ﺒﻟا ﻨﻣ ﺢﻄﺳ بﺎﺨﺘﻧا رد ﻪﺘ ﻪﺑ ﻪﺟﻮﺗ ﺮﺑ هوﻼﻋ ﺖﯿﻧﻮﺘﻨﺑ زا ﺐﺳﺎ

ﮓﻧر ﺖﯿﺻﻮﺼﺧ ﯿﻧﻮﺘﻨﺑ زا ﺢﻄﺳ ﻦﯾﺮﺘﻤﮐ ﻪﮐ هﺪﺷ ﯽﻌﺳ ﯽﯾادز

ﻪﮐ ﺖ

ياراد ﯾﺮﺘﺸﯿﺑ ﻦ ﯿﻓﺎﻔﺷ رد ﯽﯾارﺎﮐ ﺷﺎﺑ هرﺎﺼﻋ ﺖ

دﻮﺷ ﻪﺘﻓﺮﮔ رﺎﮐ ﻪﺑ ﺪ

ﺪﺷﺎﺑ يدﺎﺼﺘﻗا ﻪﯿﺟﻮﺗ ياراد ﺎﺗ .

ﯿﻨﭽﻤﻫ ﺐﺳﺎﻨﻣ ﺢﻄﺳ بﺎﺨﺘﻧا رد ﻦ

ﻪﻌﻣﺎﺟ ﺖﻣﻼﺳ ﻪﺑ pH ﺖﺳا هﺪﺷ ﻪﺟﻮﺗ

. ﯿﺗ ﺢﻄﺳ رﺎﻤ 20

مﺮﮔ

ﺮﺘﯿﻟرد ﯿﻧﻮﺘﻨﺑ ﻞﮐ دﻮﺒﻬﺑ ﺚﻋﺎﺑ ﺖ تﺎﻔﺻ

ﻞﻣﺎﺷ : ،ﺪﯿﺋﻮﻨﺗرﺎﮐ ،ﻞﮐ لﻮﻨﻓ

ﻞﯿﻓوﺮﻠﮐ ،a

ﻞﯿﻓوﺮﻠﮐ ،b

ﻞﯿﻓوﺮﻠﮐ ﻞﮐ ﺪﯿﺋﻮﻧوﻼﻓ و ﯾدﺮﮔ

ﺪ . رد ﻪﺘﺒﻟا

ﻦﺘﻓﺮﮔ ﺮﻈﻧ ﯿﺑ ﯽﯾارﺎﮐ رد هرﺎﺼﻋ pH

ﺮﺘﺸ ﯿﻬﺑ ﺢﻄﺳ ﻪﻨ ﯿﻧﻮﺘﻨﺑ ﺖ

ﺸﭼ ﯿﮕﻤ دﻮﺑ ﺮ . ﺎﺑ ﺖﺳد ﻪﺑ ﺞﯾﺎﺘﻧ هﺪﻣآ

ﯽﻣ ﻪﯿﺻﻮﺗ دﺮﺑرﺎﮐ ﺎﺑ ،ددﺮﮔ

ﺐﺳﺎﻨﻣ ﺢﻄﺳ ﺖﯿﻧﻮﺘﻨﺑ

) 20 ﻟ رد مﺮﮔ ﺮﺘﯿ

( بذﺎﺟ ﮏﯾ ناﻮﻨﻋ ﻪﺑ

نازرا ﯽﺤﻄﺳ ﻢﯿﻈﻨﺗ و

ﺐﺳﺎﻨﻣ pH ) 5 ( هرﺎﺼﻋ ، ﺶﯾاﺰﻓا ﺐﺒﺳ

ﻢﯾدﺮﮔ هرﺎﺼﻋ ﺖﯿﻓﺎﻔﺷ و ﮓﻧر ﺖﯿﻔﯿﮐ .

5 - ﻊﺑﺎﻨﻣ

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Effect of amount of bentonite and pH level on color and clarification of Stevia (Stevia rebaudiana Bertoni) extracts.

AhmadiGoragi, R. ¹, Hamidoghli, Y. ^2, Sharifzadeh, B. ³

1. M. Sc. Student, Department of Horticultural Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2. Associate Professor, Department of Horticultural Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3. Assistant Professor. Department of Computer Engineering and Engineering Science, Faculty of Engineering - East Guilan, University of Guilan, Roodsar, Iran

(Received: 2019/09/12 Accepted: 2020/04/11)

In order to investigate the effect of different levels of bentonite and pH of the extract on exposure to bentonite on the healing rate of stevia extract (Stevia rebaudiana Bertoni), a study was conducted on two factors in a completely randomized design with four replications. The first factor contains bentonite in four levels with concentrations of 0 (control), 10, 20 and 30 g / l and the second factor contains different pH of crude extract in five levels with 5.9 (control), they were 5.5, 5, 4.5 and 4, respectively. The results of analysis of variance showed that treatment with different amounts of bentonite had a significant effect on phenol, carotenoid, chlorophyll a, chlorophyll b and chlorophyll and flavonoid extracts. Also the best level of bentonite with the least amount and maximum clarification was 20 g / l with 0.07 mg / ml phenol, 0.36 mg / l carotenoid, chlorophyll a 1 mg / l, Chlorophyll b.03, Chlorophyll 3.03 mg / l and flavonoid content of 2.69 µg / ml were selected for subsequent experiment. The pH of the extract showed a significant effect on the phenol content of the extract and a significant effect on other traits. The best level of pH of the extract with the highest clarification and attention to human health, pH (5) with 0.15 mg/ml phenol, 1.42 mg / l carotenoid, 0.77 mg chlorophyll a Chlorophyll b 0.84 mg/l, chlorophyll b 1.61 mg / l and flavonoid with 2.17 µg / ml were selected. Therefore, the results of this study show that by considering appropriate amounts of dye compounds such as bentonite (20 g / l) at a suitable pH (5), the crude extract, color and transparency of stevia extract can be improved.

Keywords: Stevia rebaudiana extract, Phenol, Flavonoid, Chlorophyll.