١٧٣
زرا ﯾﺑﺎ ﯽ ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ﯿ
ﺎﻧاﻮﺗ ،ﻞﮐ ﺪ ﯾ
ﯽ دار ﯾ ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ ﭼرﺎﻗﺪﺿ ﺮﺛا و ﯽ
ﻋ ﻟﻮﻧﺎﺗا هرﺎﺼ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ
ﮏﭙﮐ ﺮﺑ ﺎﻫ ي ﻣ دﺎﺴﻓ ﻞﻣﺎﻋ ﯿ
ﻃ لﺎﻘﺗﺮﭘ هﻮ ﯽ
ﻧﺎﻣرﺎﺒﻧا ﯽ
ﻔﻄﺼﻣ ﯽ ﺘﻤﺣر ﯽ ﻨﺟ ﯿ دﺎﺑآﺪ
1
،*
ﻠﻋ زوﺮﻬﺑ ﯿ ﻧﺎﻬﺒﻬﺑ هداﺰ
2ﯽ دﺎﺷﻮﻧ ﺪﻤﺤﻣ ، 3
1 - دﺎﺘﺳا ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﻧﺎﺒﻏﺎﺑ ﯽ زروﺎﺸﮐ هﺪﮑﺸﻧاد ، ي
زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ ﯽ ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ
ا ، ﯾ ناﺮ .
2 - ﺘﺳا دﺎ ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ، ﯽ
ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ ﯽ
ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ
ا ، ﯾ ناﺮ .
3 - ﺸﻧاد ﯿ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ، ﯽ
ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ ﯽ
ﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﻧﺎ
ﯽ ا ، ﯾ ناﺮ .
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 17 / 08 / 1401
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 21 / 09 / 1401
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
ﭘ لﺎﻘﺗﺮ
،
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ
،
ز هرﺎﺼﻋ ﯾ لﺎﻌﻓ ﺖﺴ
،
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ
،
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ .
DOI: 10.22034/FSCT.19.132.173
DOR: 20.1001.1.20088787.1401.19.132.13.6
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ : [email protected]
ا زا فﺪﻫ ﯾ ﺳرﺮﺑ ﻪﻌﻟﺎﻄﻣ ﻦ ﯽ
ﻟﺎﻧآ ﯿ ﻓ ﺰ ﯿ ﺷﻮﺘ ﯿﻤ ﯿﺎ ﯾ ﯽ ﻟﺎﻌﻓ و ﯿ ﺖ ﺎﻫ ي ﭼرﺎﻗﺪﺿ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﺮ
ﻟﺎﮕﻨﺑ ﯽ ) Ficus benghalensis (
ﻮﺳ ﺪﺷر ﺮﺑ ﯾﻪ
ﺎﻫ ي رﺎﻗ ﭼ ﯽ ﺑ ﯿ رﺎﻤ ي ﻣ دﺎﺴﻓ ﻞﻣﺎﻋ از ﯿ
لﺎﻘﺗﺮﭘ هﻮ
ﻃ ﯽ ﻧﺎﻣرﺎﺒﻧا ﯽ ﻨﭘ ) ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ( دﻮﺑ . ﻣﯿ شور ﻖﺑﺎﻄﻣ ﻞﮐ لﻮﻨﻓ ناﺰ
ﻟﻮﻓ ﯿ ﻦ - ﺳ ﯿ
،ﻮﺘﻟﺎﮐﻮ ﻣ ﯿ ﺋﻮﻧوﻼﻓ ناﺰ ﯿ
ﺠﻨﺳ ﮓﻧر شور سﺎﺳا ﺮﺑ ﻞﮐ ﺪ ﯽ
ﺮﻠﮐ ﯾ ﻣﻮﻟآ ﺪ ﯿﻨﯿ ﻟﺎﻌﻓ و مﻮ ﯿ ﺖ
ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ شور سﺎﺳا ﺮﺑ ﺎﻫ
ي دار رﺎﻬﻣ ﯾ دازآ لﺎﮑ و DPPH
زرا درﻮﻣABTS
ﯾﺑﺎ ﯽ راﺮﻗ
ﺖﻓﺮﮔ . شور ﺎﻫ ي ﻔﻠﺘﺨﻣ ﯽ ) دﯾ د ﮏﺴ ﯾﻔ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ،رﺎﮔآ نژﻮ ﯽ
چرﺎﻗ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﺪﺷر ﺸﮐ
ﯽ ( اﺮﺑ ي زرا ﯾﺑﺎ ﯽ ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﺮ
ﻟﺎﮕﻨﺑ ﯽ ﺖﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ .
ﻣﯿ ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ناﺰ ﯿ
ﺗﺮﺗ ﻪﺑ هرﺎﺼﻋ ﻞﮐ ﺪ ﯿ
ﺐ mg GAE/g
62 / 1 49± / 110 و mg QE/g 57
/ 1 60± / 62 دﻮﺑ . ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺎﭘ ﺮﺑ ﯾ شور ﻪ ﺎﻫ ي دار رﺎﻬﻣ ﯾ لﺎﮑ و DPPH ﺗﺮﺗ ﻪﺑ ABTS
ﯿ لدﺎﻌﻣ ﺐ 46
/ 0
±
56 / 48 و 38 / 0 20± / 57 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ دﺮﮔ هﺪﻫﺎﺸﻣ ﺮﺘ ﯾ
ﺪ . ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ نﻮﻣزآ ﺞ ﺎﻫ ي د ﯾ ﮏﺴ
دﯾ ﻔﯿ ﮔآ ﮏﻫﺎﭼ و رﺎﮔآ نژﻮ ﻟﺎﻌﻓ ،رﺎ
ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻮﺳ و دﻮﺑ ﺖﻈﻠﻏ ﻪﺑ ﻪﺘﺴﺑاو هرﺎﺼﻋ ﯾﻪ
ﺎﻫ ي
ﭼرﺎﻗ ﻨﭘﯽ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ﺗﺮﺗ ﻪﺑ ﯿ ﺮﺘﻤﮐ ﺎﺑ ﺐ ﯾ
ﺑ و ﻦ ﯿ ﺮﺘﺸ ﯾ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦ
موﺎﻘﻣ ،ﺪﺷر مﺪﻋ ﺮﺗ
ﯾ سﺎﺴﺣ و ﻦ ﺮﺗ
ﯾ ﻪﻧﻮﮕﻨ ﺪﻧدﻮﺑ هرﺎﺼﻋ ﺮﺑاﺮﺑ رد ﺎﻫ .
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
اﺮﺑ ي ﻮﺳ ﯾﻪ ﺎﻫ ي ﺗﺮﺗ ﻪﺑ قﻮﻓ ﯿ
ﺐ 16 و 8 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﺮﺘ ﯽ
512 و
128 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺪﻣآ ﺖﺳﺪﺑ ﺮﺘ .
ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ
،ﺞ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﻤﻬﻣ ﻊﺒﻨﻣ ﯽ زا
ﮐﺮﺗ ﯿ ﺘﻧآ تﺎﺒ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﭼرﺎﻗ ﺪﺿ و ﯽ
و ﺖﺳا ﻣ ﯽ ﺪﻧاﻮﺗ اﺮﺑ ي اﺰﻓا ﯾ رﺎﮔﺪﻧﺎﻣ ﺮﻤﻋ ﺶ ي
تﻻﻮﺼﺤﻣ
ﻧﺎﺒﻏﺎﺑ ﯽ دﻮﺷ هدﺎﻔﺘﺳا .
ﻪﻠﺠﻣ
مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ
www.fsct.modares.ac.ir :ﻪﻠﺠﻣﺖﯾﺎﺳ
ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ
١٧٤
1 - ﻪﻣﺪﻘﻣ
تﺎﺒﮐﺮﻣ ﯾﮑ ﯽ ﻣ تﻻﻮﺼﺤﻣ زا ﯿ
ﻤﻫا زا ﻪﮐ ﺖﺳا نﺎﻬﺟ رد هﻮ ﯿ
ﺖ
دﺎﺼﺘﻗا ي ﻻﺎﺑ ﯾ ﯽ ﻟﻮﺗ و ﺖﺳا رادرﻮﺧﺮﺑ ﯿ
ﺮﺳاﺮﺳ رد نآ ﻪﻧﻻﺎﺳ ﺪ
لﺎﺳ رد نﺎﻬﺟ 2010
ﺑ ﯿ زا ﺶ 123 ﻣ ﯿﻠ ﯿ ﺖﺳا هﺪﺷ دروآﺮﺑ ﻦﺗ نﻮ .
نآ ﺮﺗﻻﺎﺑ ﺎﻫ ﯾ ﻣ لﻮﺼﺤﻣ شزرا ﻦ ﯿ
ترﺎﺠﺗ رد ار هﻮ ﺑ ﯿ
ﻦ ﻠﻠﻤﻟا ﯽ
ﺪﻧراد . ﻣ زا ﯿ ﻪﻧﻮﮔ نﺎ ﺎﻫ ي ار ﯾ لﺎﻘﺗﺮﭘ ،هﺪﺷ ﺖﺸﮐ ﺞ )
Citrus
sinensis ( هﺪﻤﻋ ﺶﺨﺑ ا
ي ﻟﻮﺗ زا ﯿ ﮑﺸﺗ ار تﺎﺒﮐﺮﻣ ﺪ ﯿ
ﻤﻠ ﯽ و ﺪﻫد
ﺮﻘﺗ ﯾ ًﺎﺒ 55 ﻟﻮﺗ زا ﺪﺻرد ﯿ
ﻧﺎﻬﺟ تﺎﺒﮐﺮﻣ ﺪ ﯽ
صﺎﺼﺘﺧا دﻮﺨﺑ ار
ﻣ ﯽ ﺪﻫد ] 1 [.
ﺬﭘ ﯾ ﺑ ترﺎﺠﺗ رد لﺎﻘﺗﺮﭘ شﺮ ﯿ
ﻦ ﻠﻋ ﻞﻠﻤﻟا ﯿ ﺬﭘدﺎﺴﻓ ﻢﻏﺮ ﯾﺮ
ي ،ﻻﺎﺑ
ﻟد ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ ﯿ
ا ﻞ اﺬﻏ شزر ﯾ ﯽ ﻧﺎﻣرد و ﯽ ﻻﺎﺑ ي ﺪﺷﺎﺑ نآ .
وﺎﺣ لﺎﻘﺗﺮﭘ ﯾﻤ
ﯿ ﻻﺎﺑ ناﺰ ي و ﯾ ﻣﺎﺘ ﯿ ﻦ ﻞﺑﺎﻗ راﺪﻘﻣ ،C ﻬﺟﻮﺗ
ﯽ
وﯾ ﻣﺎﺘ ﯿ ﻦ ﻓ و تﻻﻮﻓ ،A ﯿ
ﺮﺤﺗ رد ﻪﮐ ﺖﺳاﺮﺒ ﯾ
دﺮﮑﻠﻤﻋ ﮏ
لﻮﺒﻠﮔ ﺎﻫ ي ﻔﺳ ﯿ ﺳ رد نﻮﺧ ﺪ ﯿ
ا ﻢﺘﺴ ﯾ ﻨﻤ ﯽ ﮑﺸﺗ ، ﯿ ،ناﻮﺨﺘﺳا ﻞ
ﻟﻮﺗ ،ﻢﺸﭼ ﺖﻣﻼﺳ ﯿ
ﺪ ﺑ ﺮﻄﺧ ﺶﻫﺎﮐ ،DNA ﯿ
رﺎﻤ ي ﺎﻫ ي
ﺒﻠﻗ ﯽ - ﻗوﺮﻋ ﯽ ﻏ و ﯿ راد ﺶﻘﻧ هﺮ ﺪﻧ
] 2 , 3 [.
راﺪﻬﮕﻧ لﻮﻃ رد ي
ﮐ ، ﯿﻔ ﯿ ﺖ ﻣﯿ هﻮ ﺎﻫ ﻣ ﺶﻫﺎﮐ ﯽ ﯾ ﺘﻧ رد و ﺪﺑﺎ ﯿ
ﻪﺠ
ﻢﻌﻃ ﺎﻫ ي ﻏ ﯿ ﺑ ﺢﻄﺳ نﺪﺷ مﺮﻧ ،هﺮﻈﺘﻨﻣﺮ ﯿ
ﻧوﺮ ﯽ
، هﻮﻬﻗ ا ي زا ،نﺪﺷ
ﺰﺠﺗ و بآ نداد ﺖﺳد ﯾ
ﺎﻫ ﺖﻓﺎﺑ ﻪ ي
ﺤﻄﺳ ﯽ ا ﯾ ﻣدﺎﺠ ﯽ دﻮﺷ .
ا ﺮﺑ هوﻼﻋ ﯾ اﺮﺷ ،ﻦ ﯾ راﺪﻬﮕﻧ ﻂ ي ﻧ ﯿ ﭼرﺎﻗ رﻮﻠﻓ مﻮﺠﻫ ﺰ ﯽ
ﻟﻮﺗ و ﯿ ﺪ
ﺎﻣ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ ﻬﺴﺗ ار ﻦ ﯿ ﻣ ﻞ ﯽ ﻪﮐ ،ﺪﻨﮐ ﯾ ﮏ ﻪﺑ هﺪﻤﻋ ﻞﮑﺸﻣ وﯾ
ﺮﺑ هﮋ
ﺬﻐﺗ صاﻮﺧ ﯾﻪ
ا ي ﻣ ﯿ هﻮ ﺖﺳا ﺎﻫ . ﺘﻧ رد ﯿ ﻟﻮﺗ،ﻪﺠ ﯿ ﺎﻣ ﺪ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ ﻦ
ﻟﻮﺗ ﯿ ﻪﻧﻮﮔ ﺪ ﺎﻫ ي ﺴﮐا ﯿ ﻮﻘﺗ ار لﺎﻌﻓ نﮋ ﯾ
ﻣ ﺖ ﯽ ﻣ ﻪﮐﺪﻨﮐ ﯽ ﺮﺠﻨﻣ ﺪﻧاﻮﺗ
ﺴﮐاﺮﭘ ﻪﺑ ﯿ ﺳاﺪ ﯿ ﻟ نﻮ ﯿﭙﯿ ﺪﯾ هﺎﺗﻮﮐ ﻮ رﺎﮔﺪﻧﺎﻣ نﺎﻣز نﺪﺷ ﺮﺗ ي
ﻣ ﯿ هﻮ ﺎﻫ
ﺬﭘ ﺐﻗﺎﻌﺘﻣ ﺶﻫﺎﮐ هاﺮﻤﻫ ﻪﺑ ﯾ
فﺮﺼﻣ ﻂﺳﻮﺗ لﻮﺼﺤﻤﺷﺮ هﺪﻨﻨﮐ
ددﺮﮔ . ﺮﺑ هوﻼﻋ ﺬﻐﺗ رﺮﺿ ﯾﻪ ا ي ﮔدﻮﻟآ ، ﯽ ﭼرﺎﻗ ﯽ ﺖﺷادﺮﺑ زا ﺲﭘ
اﺰﻓا ﺎﺑ ﺖﺳا ﻦﮑﻤﻣ ﯾ
ﺶ ﺰﻫ ﯾ ﻪﻨ ﺎﻫ ي ﻞﻤﺣ ﺧذ و ﻞﻘﻧو ﯿ
هﺮ زﺎﺳ ي ،
رازﺎﺑ شزرا ﯾﻤ
ﯿ هﻮ ﺪﻫد ﺶﻫﺎﮐ زا ار ﺎﻫ ]
4 [.
ﺎﻫ هﺪﻧراﺪﻬﮕﻧ ي
ﻋﻮﻨﺼﻣ ﯽ ﯾ ﻧﻮﻔﻋﺪﺿ ﺎ ﯽ هﺪﻨﻨﮐ ﺎﻫ ي ﺷ ﯿﻤ ﯿﺎﯾ ﯽ
اﺮﺑ ًﻻﻮﻤﻌﻣ ي ﭼرﺎﻗ دﺎﺴﻓ ﺎﺑ هزرﺎﺒﻣ ﯽ
ﮔدﻮﻟآ و ﯽ ﺎﻣ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ ﻦ
ﻣﯿ هﻮ ﻣ هدﺎﻔﺘﺳا ﺎﻫ ﯽ
ﺪﻧﻮﺷ . ﻧﻮﻔﻋﺪﺿ ﯽ هﺪﻨﻨﮐ ﺎﻫ ي وﺎﺣ ي و ﺮﻠﮐ
ﻫﯿ ﺮﻠﮐﻮﭙ ﯾ ﺜﮑﺗ ﺶﻫﺎﮐ رد ﺖ ﯿ
ﻧ ﺮﺛﺆﻣ ناﺪﻨﭼ چرﺎﻗ ﺮ ﯿ
ﺪﻨﺘﺴ .
اﺎﺑ ﯾ ﻦ ﺑ هدﺎﻔﺘﺳا ،لﺎﺣ ﯿ
نآ ﺪﺣ زا ﺶ ﺮﺤﺗ ﺚﻋﺎﺑ ﺖﺳا ﻦﮑﻤﻣ ﺎﻫ
ﯾ ﮏ
ﺴﻔﻨﺗ تﻼﮑﺸﻣ ،ﺖﺳﻮﭘ ﯽ
ﺷراﻮﮔ و ﯽ دﻮﺷ . ا ﺮﺑ هوﻼﻋ ﯾ ،نزا ،ﻦ
ﺴﮐاﺮﭘ ﯽ ﺘﺳا ﯿ ﺳا ﮏ ﯿ ﺳا ،ﺪ ﯿ ﺎﻫﺪ ي ﻟآ ﯽ ﻔﻟﻮﺳ و ﯿ ﻫ ﺪ ﯿ ﻪﺑ ردﺎﻗ نژورﺪ
ﺘﺳد ﯿﺑﺎ ﯽ ﻪﺑ ﻧ رﺎﻬﻣ ﺮﺜﮐاﺪﺣ ﯿ
ﻨﭽﻤﻫ و ﺪﻨﺘﺴ ﯿ
اراد ﻦ ي ضراﻮﻋ
ﺒﻧﺎﺟ ﯽ ﻤﺳ و ﯿ ﺪﻨﺘﺴﻫ هﻮﻘﻟﺎﺑ ﺖ ]
5 [.
اﺮﺑﺎﻨﺑ ﯾ ﻧ ﻦ ﯿ ﻪﺑ زﺎ ﯾ ﮐﺮﺗ ﻦﺘﻓﺎ ﯿ تﺎﺒ ﺒﻃ
ﯿﻌ ﯽ ﻣ ﻪﮐ ﯽ ﺎﺟ ﺪﻨﻧاﻮﺗ ﯾ ﺰﮕ ﯾ ﮐﺮﺗ ﻦ ﯿ ﻋﻮﻨﺼﻣ تﺎﺒ ﯽ
ﺪﭘ ،ﺪﻧﻮﺷ ﯾ ﺪ
ور نﺎﻘﻘﺤﻣ و ﺖﺳا هﺪﻣآ ي
ﯾ ﮐﺮﺗ ﻊﺑﺎﻨﻣ ﻦﺘﻓﺎ ﯿ
ﺗﺎﺒ ﯽ ﻟﺎﻌﻓ ﺎﺑ ﯿ ﺖ ﺎﻫ ي
ﺑﯿ ژﻮﻟﻮ ﯾﮑ ﯽ ا ﻪﮐ ﯾ ﻦﻤ ﺤﻣ راﺪﺘﺳود و ﺮﺗ ﯿ
ﻂ ز ﯾ ﺴﻫ ﺖﺴ ﺰﮐﺮﻤﺘﻣ ﺪﻨﺘ
هﺪﺷ ﺪﻧا .
ﻤﻫ رد ﯿ ﺲﻧﺎﺳا ،ﺎﺘﺳار ﻦ هرﺎﺼﻋ وﺎﻫ
ﺎﻫ ﺎﺟ ﯾ ﺰﮕ ﯾ ﺑﻮﺧ ﻦ ﯽ ﺪﻨﺘﺴﻫ
زﯾ اراد اﺮ ي ﺘﻧآ صاﻮﺧ ﯽ
ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻨﭽﻤﻫ و ﯿ ﻟﺎﻌﻓ ﻦ ﯿ ﺪﺿ ﺖ
ﭼرﺎﻗ ﯽ ﺮﺘﮐﺎﺑ ﺪﺿ و ﯾﺎﯾ
ﯽ ا ﺮﺑ هوﻼﻋ ،ﺪﻨﺘﺴﻫ ﯾ
ﺒﻃ ،ﻦ ﯿﻌ ﯽ و هدﻮﺑ
وراد و اﺬﻏ نﺎﻣزﺎﺳ ﻂﺳﻮﺗ ي
ا ﯾ ﮐﺮﺗ ناﻮﻨﻌﺑ هﺪﺤﺘﻣ تﻻﺎ ﯿ
تﺎﺒ
اﯾ هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﻦﻤ ﺪﻧا
.ا ﯾ ﺮﺗ ﻦ ﮐﯿ ﻤﺗﺎﺒ ﯽ اﺮﺑ ﺪﻨﻧاﻮﺗ ي ﺎﻫاﺬﻏ ﻪﻌﺳﻮﺗ ي
دﺮﮑﻠﻤﻋ ي ﺪﺟ ﯾ ﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ ﺪ ﯿ
ﺪﻧﺮ نآ زا هدﺎﻔﺘﺳا و ﺎﻫ
ﻣ ﯽ ﺪﻧاﻮﺗ راﺪﻬﮕﻧ ﺮﻤﻋ ي
اﺬﻏ تﻻﻮﺼﺤﻣ ﯾ
ﯽ دﻮﺒﻬﺑ ار
ﺪﺸﺨﺒﺑ ] 6 - 8 [.
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ) Ficus benghalensis (،
هداﻮﻧﺎﺧ زا
Moraceae
، ﯾ وراد ﻢﻬﻣ ﺖﺧرد ﮏ ﯾ
ﯽ رد ﻪﮐ ﺖﺳا هﺪﻨﮐاﺮﭘ و
ﻣ ﺪﻨﻫ ﻒﻠﺘﺨﻣ ﻖﻃﺎﻨ ﯾ
ﺖﻓﺎ ﻣ ﯽ دﻮﺷ . ﻪﻧﻮﮔ ﺎﻫ ي ا ﯾ اراد هداﻮﻧﺎﺧ ﻦ ي
ﻟﺎﻌﻓ ﯿ دﺪﺿ ﺖ ﯾ ﺘﺑﺎ ﯽ ﺑﺎﻬﺘﻟاﺪﺿ ، ﯽ
رﻮﻣﻮﺗﺪﺿ ، ي
ﻧﺎﻃﺮﺳﺪﺿ ، ﯽ
،
ﻟﻮﻠﺳ ﻆﻓﺎﺤﻣ ﯽ
ﺘﻧآ ،دردﺪﺿ ،ﻢﺧزﺪﺿ و ﯽ
ﺴﮐا ﯿ ﺑﺮﭼ ﺶﻫﺎﮐ ،ناﺪ ﯽ
ﻫﺪﺿ ،نﻮﺧ ﯿ
ﻠﮔﺮﭙ ﯿ ﻤﺴ ﯽ ﺪﻨﺘﺴﻫ ﺮﺑ ﺐﺗ و .
ﮐﺮﺗ ﯿ ﻓ تﺎﺒ ﯿ ﺷﻮﺘ ﯿﻤ ﯿﺎﯾ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ترﺎﺒﻋ ﺗور زا ﺪﻧا ﯿ
1ﻦ ﺮﻓ ، ﯾ ﻟﺪ ﯿ
2ﻦ لوﺮﺘﺳﻮﺴﮐارﺎﺗ ، ،3
لﻮﺌﭘﻮﻟ ﻣآ ﺎﺘﺑ ،4
ﯿﺮ ﯾ
5ﻦ ﻦﻟارﻮﺴﭘ ﺎﺑ هاﺮﻤﻫ ﻦﺘﭘﺎﮔﺮﺑ ،6
ﺎﺘﺑ و 7
ﺳ ﯿ لوﺮﺘﺴ ﺘﺳﺮﺋﻮﮐ ،8
ﯿ ﻦ - 3 - زﻮﺘﮐﻻﺎﮔ ﯾ
9ﺪ ] 9 [.
ﻪﻌﻟﺎﻄﻣ ﺎﻫ ي ﻒﻠﺘﺨﻣ
ﻣ نﺎﺸﻧ ﯽ ﺪﻫد ﺠﻧا ﺲﻧﺎﺳا و هرﺎﺼﻋ ﻪﮐ ﯿ
ﻟﺎﮕﻨﺑ ﺮ ﯽ اراد ي ﻟﺎﻌﻓ ﯿ ﺖ
ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣﺪﺿ و ﯿ ﺑوﺮﮑ ﯽ ﻣ ﺮﺑاﺮﺑ رد ﯿ
ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي
ﺑﯿ رﺎﻤ ي از ﻣ ﯽ ﺪﺷﺎﺑ ] 9 [.
ا ﺎﺑ ﯾ ﺳرﺮﺑ ﺎﺑ ،لﺎﺤﻨ ﯽ
ﻤﻠﻋ ﻊﺑﺎﻨﻣ ﯽ ﺴﺑ تﺎﻌﻟﺎﻄﻣ ، ﯿ
ﻤﮐ رﺎ ﯽ درﻮﻣ رد
ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗﺪﺿ ﺖ ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺪﺷر ﺮﺑاﺮﺑ رد
چرﺎﻗ ﺎﻫ ي ﺎﺴﻓ ﻞﻣﺎﻋ نژﻮﺗﺎﭘ ﻣ د
ﯿ ﻃ لﺎﻘﺗﺮﭘ هﻮ ﯽ
ﻧﺎﻣرﺎﺒﻧا ﯽ ترﻮﺻ
ﺖﺳا ﻪﺘﻓﺮﮔ .
اﺮﺑﺎﻨﺑ ﯾ ا ،ﻦ ﯾ ﻌﺗ فﺪﻫ ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦ ﯿﯿ
ﭼرﺎﻗﺪﺿ ﺮﺛا ﻦ ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺮﺑاﺮﺑ رد ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ و
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ﺪﺷ مﺎﺠﻧا . ا ﺮﺑ هوﻼﻋ ﯾ ﻣ ،ﻦ ﯿ ،ﻞﮐ لﻮﻨﻓ ناﺰ
ﺋﻮﻧوﻼﻓ ﯿ ﻟﺎﻌﻓ و ﻞﮐ ﺪ ﯿ
ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻧ هرﺎﺼﻋ ﯿ ﺮﺑ ﺰ ﺳر ﯽ
دﺮﮔ ﯾ ﺪ.
1. Rutin 2. Friedelin 3.Taraxosterol 4. Lupeol 5. β-amyrin 6. Psoralen 7. Bergapten 8. β-sisterol
9. quercetin-3-galactoside
١٧٥
2 - شور و داﻮﻣ ﺎﻫ
2 - 1 - داﻮﻣ
ﺘﺳﺮﺋﻮﮐ ﯿ ﺳا ،ﻦ ﯿ ﻟﺎﮔ ﺪ ﯿ ،ﮏ DPPH10
11 و ﺖﮐﺮﺷ زا ABTS
ﺳ ﯿ رﺪﻟآ ﺎﻤﮕ ﯾ ﭻ ) ﺮﻣآ ﯾ ﺎﮑ ( و ﺤﻣ ﯿ ﻂ ﺎﻫ ي زوﺮﺘﺴﮐد دورﻮﺑﺎﺳ ﺖﺸﮐ
ثاﺮﺑ و رﺎﮔآ كﺮﻣ ﺖﮐﺮﺷ زا
) نﺎﻤﻟآ ( ﻬﺗ ﯿ ﺪﻧﺪﺷ ﻪ .
2 - 2 - هرﺎﺼﻋ جاﺮﺨﺘﺳا
ندوﺰﻓا ﺎﺑ 50 ﺠﻧا ردﻮﭘ مﺮﮔ ﯿ
ﻟﺎﮕﻨﺑ ﺮ ﯽ ﻪﺑ 250 ﻣ ﯿﻠ ﯽ ﻟﯿ ،لﻼﺣ ﺮﺘ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﻬﺗ ﯿ ﺪﺷ ﻪ . تﺪﻣ ﻪﺑ جاﺮﺨﺘﺳا 48
ﺎﻣد رد ﺖﻋﺎﺳ ي
ﺤﻣ ﯿ ﺬﻏﺎﮐ ﻂﺳﻮﺗ گﺮﺑ ردﻮﭘ و هرﺎﺼﻋ طﻮﻠﺨﻣ ﺲﭙﺳ و مﺎﺠﻧا ﻂ
ﻓﺎﺻ ﯽ ﻓ ﻦﻤﺗاو ﯿ دﺮﮔ ﺮﺘﻠ ﯾﺪ . رد ﺲﭙﺳ ﻞﺻﺎﺣ لﻮﻠﺤﻣ 3000
ﺮﺑاﺮﺑ
ﺸﻧاﺮﮔ بﺎﺘﺷ ﯽ
تﺪﻣ ﻪﺑ 10 ﻗد ﯿ ﺮﺘﻧﺎﺳ ﻪﻘ ﯾﻔ ﯿ ژﻮ ﺪﺷ . ﺎﻬﻧ رد ﯾ ﺖ
ﻪﺑ ﻟﻮﺗ رﻮﻈﻨﻣ ﯿ ﻠﻏ و لﻼﺣ نوﺪﺑ هرﺎﺼﻋ ﺪ ﯿ
زا هدﺎﻔﺘﺳا ﺎﺑ لﻮﻠﺤﻣ ،ﻆ
ﺸﺧﺮﭼ رﻮﺗاﺮﭘاوا ﯽ
ﻸﺧ ﺖﺤﺗ ﻠﻐﺗ
ﯿ ﻆ دﺮﮔ ﯾ ﺪ . ﻠﻐﺗ هرﺎﺼﻋ ﯿ ﻆ هﺪﺷ
ﺷ فوﺮﻇ رد ﺲﭙﺳ ﯿ
ﻪﺸ ا ي ﺗ ﯿ ﺎﻣد رد هﺮ ي 4 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
داﺮﮔ
راﺪﻬﮕﻧ ي دﺮﮔ ﯾﺪ ] 10 [.
2 - 3 - هزاﺪﻧا ﮔﯿ ﺮ ي ﻞﮐ لﻮﻨﻓ
اﻮﺘﺤﻣ ي ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻞﮐ لﻮﻨﻓ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﻟﻮﻓ شور ﺎﺑ ﯿ
ﻦ -
ﺳ ﯿ هزاﺪﻧا ﻮﺘﻟﺎﮐﻮ ﮔﯿ
ﺮ ي ﺪﺷ . ﻪﺑ ،ﻪﺻﻼﺧ رﻮﻃ 8/
0 ﻣ ﯿﻠ ﯽ ﻟﯿ زا ﺮﺘ
ﺖﻈﻠﻏ ﺎﺑ هرﺎﺼﻋ 50
ﻣ ﯿ ﻣ ﺮﺑ مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺎﺑ ﺮﻄﻘﻣ بآ رد ﺮﺘ 1/
0
ﻣﯿ ﻠ ﯽ ﻟﯿ ﻟﻮﻓ فﺮﻌﻣ ﺮﺘ ﯿ
ﻦ - ﺳ ﯿ ﻗر ﻮﺘﻟﺎﮐﻮ ﯿ ﻖ و ﺪﺷ طﻮﻠﺨﻣ ًﻼﻣﺎﮐ هﺪﺷ
تﺪﻣ ﻪﺑ 3 ﻗد ﯿ رد ﻪﻘ ﺎﻣد ي راﺪﻬﮕﻧ قﺎﺗا ي
ﺪﺷ . ،نآ زا ﺲﭘ
1/
0 ﻣﯿ ﻠ ﯽ ﻟﯿ ﺪﺳ تﺎﻨﺑﺮﮐ ﺮﺘ ﯾ
ﻢ 10 ﻪﻧﻮﻤﻧ طﻮﻠﺨﻣ ﺮﻫ ﻪﺑ ﺪﺻرد
تﺪﻣ ﻪﺑ و ﺪﺷ ﻪﻓﺎﺿا 30
ﻗد ﯿ د ﻪﻘ ﯾ رﺎﺗ رد ﺮﮕ ﯾﮑ ﯽ ﺎﻣد رد ي قﺎﺗا
راﺪﻬﮕﻧ ي دﺮﮔ ﯾ ﺪ . رد ﻪﻧﻮﻤﻧ بﺬﺟ ﺲﭙﺳ 760
ﺮﺘﻣﻮﻧﺎﻧ
هزاﺪﻧا ﮔﯿ ﺮ ي ﺪﺷ . ﻟﺎﮔ ﯿ ﺳا ﮏ ﯿ و ﺪﺷ هدﺎﻔﺘﺳا دراﺪﻧﺎﺘﺳا ناﻮﻨﻋ ﻪﺑ ﺪ
ﺎﺘﻧ ﯾ ﻣ ترﻮﺼﺑ ﺞ ﯿﻠ
ﯽ ﺳا لدﺎﻌﻤﻣﺮﮔ ﯿ
ﻟﺎﮔ ﺪ ﯿ ﮏ ) (GAE مﺮﮔ رد
هرﺎﺼﻋ ) mg GAE/g (
ﺑﯿ ﺪﺷ نﺎ ] 11 [.
2 - 4 - هزاﺪﻧا ﮔﯿ ﺮ ي ﺋﻮﻧوﻼﻓ ﯿ ﻞﮐ ﺪ
اﺮﺑ ي ﻌﺗ ﯿﯿ ﻤﻨ ﯿ ﺋﻮﻧوﻼﻓ ناﺰ ﯿ
،ﻞﮐ ﺪ 1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﮔ هرﺎﺼﻋ ﺮﺘ ﯿ
ﻫﺎ ﯽ ﺎﺑ 1
ﻣﯿ ﻠ ﯽ ﻟﯿ ﻟﻮﻧﺎﺘﻣ لﻮﻠﺤﻣ ﺮﺘ ﯽ
AlCl3
2 ﺪﺷ طﻮﻠﺨﻣ ﺪﺻرد .
زا ﺲﭘ
راﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ ي
ﺎﻣد رد ي تﺪﻣ ﻪﺑ قﺎﺗا 15
ﻗد ﯿ بﺬﺟ ،ﻪﻘ
ﻣ لﻮﻃ رد ﺶﻨﮐاو طﻮﻠﺨ جﻮﻣ
430 هزاﺪﻧا ﺮﺘﻣﻮﻧﺎﻧ ﮔﯿ
ﺮ ي ﺪﺷ .
ﺘﺳﺮﺋﻮﮐ ﯿ ﻦ ﻨﺤﻨﻣ ﻢﺳر ﺖﻬﺟ ﯽ
دراﺪﻧﺎﺘﺳا )
0 - 50 ﻣ ﯿﻠ ﯽ رد مﺮﮔ
10. 2,2-diphenyl-1-picrylhydrazyl
11. 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
ﻟﯿ ﺮﺘ ( هدﺎﻔﺘﺳا دﺮﮔ ﯾﺪ . اﻮﺘﺤﻣ ي ﺋﻮﻧوﻼﻓ ﯿ ترﻮﺻ ﻪﺑ هرﺎﺼﻋ ﻞﮐ ﺪ
ﻣﯿ ﻠ ﯽ مﺮﮔ ﺘﺳﺮﺋﻮﮐ لدﺎﻌﻣ ﯿ
ﻦ ﮏﺸﺧ نزو رد
هرﺎﺼﻋ ) mg QE/g (
ﺪﺷ شراﺰﮔ ]
12 [.
2 - 5 - ﺎﻌﻓ ﻟﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ
ا رد ﯾ ﻟﺎﻌﻓ ،ﻪﻌﻟﺎﻄﻣ ﻦ ﯿ
دار رﺎﻬﻣ ﺖ ﯾ
لﺎﮑ ﺎﻫ ي و DPPH ABTS
اﺮﺑ ي ﻌﺗ ﯿﯿ ﻦ ﺮﺛا ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ
ﺳرﺮﺑ ﯽ دﺮﮔ ﯾ ﺪ ] 13 , 14 [.
اﺮﺑ ي ﻌﺗ ﯿﯿ ﻟﺎﻌﻓ ﻦ ﯿ ﺘﻧﺂﺘ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺐﺴﺣﺮﺑ ،DPPH 50 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﺘ
ﻫرﺎﺼﻋ ﯿ لﻮﻠﺤﻣ ﺎﺑ لﺮﺘﻨﮐ ﺎ 12
/0 ﻣ ﯿﻠ ﯽ رﻻﻮﻣ ﻟﻮﻧﺎﺗا ﯽ DPPH
) 5 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﺪﺷ طﻮﻠﺨﻣ .
ﺎﻣد رد هﺪﻣآ ﺖﺳﺪﺑ لﻮﻠﺤﻣ ي
25
ﺘﻧﺎﺳ ﻪﺟرد ﯽ تﺪﻣ ﻪﺑ داﺮﮔ 30
ﻗد ﯿ راﺪﻬﮕﻧ ﻪﻘ ي نآ بﺬﺟ و ﺪﺷ
لﻮﻃ رد جﻮﻣ 517 دﺮﮔ ﺖﺋاﺮﻗ ﺮﺘﻣﻮﻧﺎﻧ ﯾﺪ
.
ﻌﺗ ﺖﻬﺟ ﯿﯿ ﻟﺎﻌﻓ ﻦ ﯿ رﺎﻬﻣ ﺖ لﻮﻠﺤﻣ ،ABTS
و ABTS
K2S2O8
اﺮﺑ اﺪﺘﺑا رد ي
ﻟﻮﺗ ﯿ ﺗﺎﮐ لﻮﻠﺤﻣ ﺪ ﯿ
ﻧﻮ ﯽ دار ﯾ لﺎﮑ ABTS
ﺪﺷ طﻮﻠﺨﻣ ﻢﻫ ﺎﺑ ﺪﻧ
. ،نآ زا ﺲﭘ 1/
0 ﻣﯿ ﻠ ﯽ ﻟﯿ هرﺎﺼﻋ ﺮﺘ ﯾ لﺮﺘﻨﮐ ﺎ
ﺎﺑ 9/
3 ﻣ ﯿﻠ ﯽ ﻟﯿ دار لﻮﻠﺤﻣ ﺮﺘ ﯾ
لﺎﮑ بﺬﺟ و ﺪﺷ طﻮﻠﺨﻣ ABTS
رد نآ 734 دﺮﮔ ﺖﺒﺛ ﺮﺘﻣﻮﻧﺎﻧ ﯾﺪ
.
ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ز لﻮﻣﺮﻓ ﻖﺑﺎﻄﻣ هرﺎﺼﻋ ﯾ
و ﻪﺒﺳﺎﺤﻣ ﺮ
ﺐﺴﺣ ﺮﺑ ﺲﭙﺳ IC50
) ﻣﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ ( دﺮﮔ شراﺰﮔ ﯾﺪ
.
2 - 6 - ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﭼرﺎﻗ ﺪﺿ ﺮﺛا ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ شور سﺎﺳا ﺮﺑ ﺎﻫ
ي
دﯾ د ﮏﺴ ﯾﻔ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ،رﺎﮔآ نژﻮ ﯽ
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﯽ
ﺳرﺮﺑ ﯽ دﺮﮔ ﯾﺪ .
2 - 6 - 1 - د ﯾ د ﮏﺴ ﯾﻔ ﯿ رﺎﮔآ نژﻮ
ا رد ﯾ د ،نﻮﻣزآ ﻦ ﯾ
ﮏﺴ ﺎﻫ ي تﺪﻣ ﻪﺑ ﮏﻧﻼﺑ 15
ﻗد ﯿ هرﺎﺼﻋ رد ﻪﻘ
ﺮﺘﺳا ﯾ ﻞ ) 20 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﺘ ( ﻪﻃﻮﻏ ﺪﻧﺪﺷ رو . د ﺲﭙﺳ ﯾ ﮏﺴ ﺎﻫ ﯾ ﮏﻧﻼﺒ
ور ي ﺤﻣ ﯿ وﺎﺣ رﺎﮔآ زوﺮﺘﺴﮐد دورﻮﺑﺎﺳ ﺖﺸﮐ ﻂ ي
ﻮﺳ ﯾﻪ ﺎﻫ ي
ﭼرﺎﻗ ﯽ ﺪﻧﺪﺷ هدادراﺮﻗ .
راﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ زا ﺲﭘ ي
ﺮﺘﭘ ي دﯾ رد ﺶ
ﺎﻣد ي 27 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ 72
ﺣﺎﻧ ،ﺖﻋﺎﺳ ﯿﻪ
رادزﺎﺑ ي
)ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( د فاﺮﻃا ﯾ ﮏﺴ ﺎﻫ هزاﺪﻧا ﮔﯿ ﺮ ي ناﻮﻨﻋ ﻪﺑ و ﺴﻧﺎﺘﭘ
ﯿ ﺪﺿ ﻞ
ﭼرﺎﻗ ﯽ ﺪﺷ شراﺰﮔ هرﺎﺼﻋ ]
6 , 10،8 [.
2 - 6 - 2 - رﺎﮔآ ﮏﻫﺎﭼ
اﺮﺑ ي ا ﯾ ﺠﻧا هرﺎﺼﻋ ،رﻮﻈﻨﻣ ﻦ ﯿ
ﻟﺎﮕﻨﺑ ﺮ ﯽ ) 20 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﺘ ( ﻪﺑ
ﮏﻫﺎﭼ ﺎﻫ ﯾ ﯽ ) ﺮﻄﻗ 6 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ور ًﻼﺒﻗ ﻪﮐ ي
دورﻮﺑﺎﺳ ﺢﻄﺳ
١٧٦
ﺮﺘﭘ رد رﺎﮔآ زوﺮﺘﺴﮐد ي
دﯾ ﺶ اﯾ ﻪﺑ هدﻮﻟآ و دﻮﺑ هﺪﺷ دﺎﺠ
ﻮﺳ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ﺪﺷ ﻪﻓﺎﺿا ،دﻮﺑ .
ﺮﺘﭘ ي دﯾ ﺶ تﺪﻣ ﻪﺑ 72
ﺎﻣد رد ﺖﻋﺎﺳ ي
27 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
راﺪﻬﮕﻧ داﺮﮔ ي
ﺲﭙﺳ و ﺪﺷ
رادزﺎﺑ ﻖﻃﺎﻨﻣ ي
ﭼرﺎﻗ ﺪﺿ ﺮﺛا ناﻮﻨﻋ ﻪﺑ ﯽ
دﺮﮔ ﺖﺒﺛ هرﺎﺼﻋ ﯾﺪ
] 6 , 10،8 [.
2 - 6 - 3 - ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
ﮔﺪﻨﺸﮐ و ﯽ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
ادوﺮﮐﺎﻣ شور ﺎﺑ ﯾ
ثاﺮﺑ ﻦﺷﻮﻠ
ﺳرﺮﺑ ﯽ دﺮﮔ ﯾﺪ . ﺖﻈﻠﻏ ﺎﻫ ي ﻟاﻮﺘﻣ ﯽ ) 1 ، 2 ، 4 ، 8،
16 ، 32 ، 64 ،
128 ، 256 و ، 512 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﺤﻣ رد هرﺎﺼﻋ ﯿ
ﻂ
ﺮﻃ زا ثاﺮﺑ زوﺮﺘﺴﮐد دورﻮﺑﺎﺳ ﯾ
ﻓ ﻖ ﯿ ﺎﻫﺮﺘﻠ ﯾ ﮕﻧﺮﺳ ﺮﺴ ﯽ 45 /0
ﻣﯿ ﺮﺘﻣوﺮﮑ ي ﺮﺘﺳا ﯾ ﻪﻟﻮﻟ رد و هﺪﺷ ﻞ ﺎﻫ
ي ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ ر ﯾ ﺪﺷ ﻪﺘﺨ .
ﺲﭙﺳ ﺴﻧﺎﭙﺳﻮﺳ ﯿ نﻮ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ﻪﻟﻮﻟ ﺮﻫ نورد دراﺪﻧﺎﺘﺳا
دﺮﮔ ﻪﻓﺎﺿا ﯾﺪ . ﻪﻟﻮﻟ ﺲﭙﺳ ﺎﻫ ي وﺎﺣ ي ﺖﺸﮐ ﺎﻣد رد هﺪﺷ ي
27
ﺘﻧﺎﺳ ﻪﺟرد ﯽ تﺪﻣ ﻪﺑ داﺮﮔ 72
ﺪﻧﺪﺷ هداد راﺮﻗ ﺖﻋﺎﺳ .
زا ﺲﭘ
نآ ﻪﻟﻮﻟ ﻤﺸﭼ ترﻮﺻ ﻪﺑ تروﺪﮐ ﺮﻈﻧ زا ﺎﻫ ﯽ
ﺳرﺮﺑ ﯽ ﺪﺷ . ﻟوا ﯿ ﻦ
ﻪﻟﻮﻟ ا ي ﺪﺸﻧ هﺪﻫﺎﺸﻣ تروﺪﮐ نآ رد ﻪﮐ ناﻮﻨﻋ ﻪﺑ
ﺖﻈﻠﻏ ﻞﻗاﺪﺣ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد هرﺎﺼﻋ .
ﺎﺘﻧ سﺎﺳا ﺮﺑ ﯾ
ﺞ نﻮﻣزآ ﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﮔﺪﻨﻨ
ﯽ ،ﺪﺷر 100
ﻣﯿ ﻟوﺮﮑ ﯿ ﻪﻟﻮﻟ زا ﺮﺘ ﺎﻫ ي ﻣ ﺪﺷر نوﺪﺑ ﯿ
ﺑوﺮﮑ ﯽ ور ي ﺤﻣ ﯿ ﺖﺸﮐ ﻂ
ﺪﺷ هداد ﺖﺸﮐ رﺎﮔآ زوﺮﺘﺴﮐد دورﻮﺑﺎﺳ .
ﺤﻣ ،ﺪﻌﺑ ﻪﻠﺣﺮﻣ رد ﯿ
ﻂ
ﺎﻣد رد ﺖﺸﮐ ي
27 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ 72
ﺖﻋﺎﺳ
راﺪﻬﮕﻧ ي دﺮﮔ ﯾ ﺮﺘﻤﮐ و ﺪ ﯾ گﺮﻣ ﻪﺑ ﺮﺠﻨﻣ ﻪﮐ هرﺎﺼﻋ ﺖﻈﻠﻏ ﻦ
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ناﻮﻨﻋ ﻪﺑ ﺪﺷ چرﺎﻗ ﯽ
ﺮﻈﻧ رد ﻪﺘﻓﺮﮔ
ﺪﺷ ] 6 , 10،8 [.
2 - 7 - ﻟﺎﻧآ ﯿ رﺎﻣآ ﺰ ي
ﺎﺘﻧ ﯾ مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﺞ راﺰﻓا
) SPSS ﻪﺨﺴﻧ 18 ( زا هدﺎﻔﺘﺳا ﺎﺑ و
ﻟﺎﻧآ ﯿ راو ﺰ ﯾ ﺴﻧﺎ ﯿ ﮏ ﺰﺠﺗ درﻮﻣ ﻪﻓﺮﻃ ﯾ
ﻠﺤﺗ و ﻪ ﯿ و ﺖﻓﺮﮔ راﺮﻗ ﻞ
ﻣ فﻼﺘﺧا ﯿ ﮕﻧﺎ ﯿ هداد ﻦ رد ﻦﮑﻧاد نﻮﻣزآ ﺎﺑ ﺎﻫ p<0.05
ﻌﺗ ﯿﯿ ﻦ
ﺪﺷ . نﻮﻣزآ مﺎﻤﺗ ﺪﻧﺪﺷ راﺮﮑﺗ ﻪﺒﺗﺮﻣ ﻪﺳ رد ﺎﻫ
.
3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ
ﺎﺘﻧ ﯾ ﻣ ﺞ ﯿ ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ناﺰ ﯿ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻞﮐ ﺪ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ
ﻞﮑﺷ رد 1 ﺖﺳا هﺪﺷ ﻪﺋارا .
ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ اراد هرﺎﺼﻋ ،ﺞ ي
mg
GAE/g 62 /1 49± / 110 و ﻞﮐ لﻮﻨﻓ mg QE/g
57 /1
±
60 / 62 ﺋﻮﻧوﻼﻓ ﯿ دﻮﺑ ﻞﮐ ﺪ .
Fig 1 Total phenol and flavonoid contents of Ficus
benghalensis ethanolic extract.
ﻞﮑﺷ 2 ﺎﺘﻧ ، ﯾ ﻟﺎﻌﻓ ﺞ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﺮ
ﻟﺎﮕﻨﺑ ﯽ ﻣ نﺎﺸﻧ ار ﯽ ﺪﻫد . دار رﺎﻬﻣ ﻪﺑ ردﺎﻗ هرﺎﺼﻋ ﯾ
لﺎﮑ ﺎﻫ ي دازآ
ﻠﺑﺎﻗ و دﻮﺑ ﯿ دار رﺎﻬﻣ رد نآ ﺖ ﯾ
لﺎﮑ ﺎﻫ ي و DPPH ﻪﺑ ABTS
ﺗﺮﺗ ﯿ ﺐ 46 /0 56± / 48 و 38 /0 20± / 57 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ
دﻮﺑ .
Fig 2 Antioxidant activity of Ficus benghalensis ethanolic extract, based on DPPH and ABTS free
radical scavenging methods.
ﺎﺘﻧ ﯾ ﭼرﺎﻗ ﺪﺿ ﺮﺛا ﺞ ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﺮ ﻟﺎﮕﻨﺑ ﯽ سﺎﺳا ﺮﺑ
د شور ﯾ د ﮏﺴ ﯾﻔ ﯿ ﻞﮑﺷ رد رﺎﮔآ نژﻮ 3
ﺖﺳا هﺪﺷ شراﺰﮔ .
ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻮﺳ عﻮﻧ و ﺖﻈﻠﻏ ﻪﺑ ﻪﺘﺴﺑاو هرﺎﺼﻋ ﯾ
ﻪ
ﭼرﺎﻗ ﯽ دﻮﺑ . اﺰﻓا ﯾ زا هرﺎﺼﻋ ﺖﻈﻠﻏ ﺶ 15
ﻪﺑ 60 ﻣ ﯿﻠ ﯽ رد مﺮﮔ
ﻣﯿ ﻠ ﯽ ﻟﯿ اﺰﻓا ﺐﺒﺳ ﺮﺘ ﯾ
ﻨﻌﻣ ﺶ ﯽ زا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ راد 8
ﻪﺑ
10 / 17 ﻣ ﯿﻠ ﯽ رد ﺮﺘﻣ ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ و 90 /9 ﻪﺑ 80 / 19
ﻣﯿ ﻠ ﯽ رد ﺮﺘﻣ ﻨﭘ ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ دﺮﮔ ﯾﺪ . هﺪﻫﺎﺸﻣ ﻪﮐ رﻮﻄﻧﺎﻤﻫ
ﻣ ﯽ ،دﻮﺷ ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﺮﺘﻤﮐ ﺎﺑ ﯾ ﻨﭘ و ﻦ ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ
١٧٧
ﺑ ﺎﺑ ﯿ ﺮﺘﺸ ﯾ ﺗﺮﺗ ﻪﺑ ،ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦ ﯿ
موﺎﻘﻣ ﺐ ﺮﺗ ﯾ و ﻦ
سﺎﺴﺣ ﺮﺗ ﯾ ﻮﺴﻨ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﺮﺑاﺮﺑ رد ﯽ
ﺠﻧا ﯿ ﺮ
ﻟﺎﮕﻨﺑ ﯽ ﺪﻧدﻮﺑ .
Fig 3 Antifungal effect of Ficus benghalensis ethanolic extract, according to the disk diffusion
agar test.
ﯾ ﻪﺘﻓﺎ ﺎﻫ ي ﺎﺘﻧ ﻖﺑﺎﻄﻣ رﺎﮔآ ﮏﻫﺎﭼ نﻮﻣزآ ﯾ
د نﻮﻣزآ ﺞ ﯾ
ﮏﺴ
دﯾ ﻔﯿ دﻮﺑ رﺎﮔآ نژﻮ )
ﻞﮑﺷ 4 ( موﺎﻘﻣ و ﺮﺗ ﯾ و ﻦ
سﺎﺴﺣ ﺮﺗ ﯾ ﻮﺴﻨ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﺮﺑاﺮﺑ رد ﯽ
ﺠﻧا ﯿ ﺮ
ﻟﺎﮕﻨﺑ ﯽ ﺗﺮﺗ ﻪﺑ ﯿ ﻨﭘ ﺐ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ
ﻣﺪﺿ ﺮﺛا و ﺪﻧدﻮﺑ ﯿ
ﺑوﺮﮑ ﯽ اﺰﻓا ﺎﺑ هرﺎﺼﻋ ﯾ
رﻮﻄﺑ ﺖﻈﻠﻏ ﺶ
ﻨﻌﻣ ﯽ راد ي اﺰﻓا ﯾ داد نﺎﺸﻧ ﺶ .
ا ﺎﺑ ﯾ ﻪﮐ ﺖﺳا ﺮﮐذ ﻪﺑ مزﻻ لﺎﺤﻨ
گرﺰﺑ رﺎﮔآ ﮏﻫﺎﭼ نﻮﻣزآ رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ د زا ﺮﺗ
ﯾ ﮏﺴ
دﯾ ﻔﯿ دﻮﺑ رﺎﮔآ نژﻮ .
Fig 4 Antifungal effect of Ficus benghalensis ethanolic extract, according to the well diffusion
agar test.
ا ﺮﺑ هوﻼﻋ ﯾ
ﺎﺘﻧ ،ﻦ ﯾ نﻮﻣزآ ﺞ ﺎﻫ ي ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
) ﻞﮑﺷ 5 ( ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﯽ
) ﻞﮑﺷ 6 ( ﺎﺸﻧ ﻪﮐ داد ن ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ﺗﺮﺗ ﻪﺑ ﯿ ﺐ
موﺎﻘﻣ ﺮﺗ ﯾ سﺎﺴﺣ و ﻦ ﺮﺗ
ﯾ ﻮﺴﻨ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ هرﺎﺼﻋ ﺮﺑاﺮﺑ رد
ﺪﻧدﻮﺑ . ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
اﺮﺑ ي ا ﯾ ﻮﺳ ﻦ ﯾﻪ ﺗﺮﺗ ﻪﺑ ﺎﻫ ﯿ ﺐ
16 و 8 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﺮﺘ ﯽ
اﺮﺑ ي
اﯾ ﻮﺳ ﻦ ﯾﻪ ﺗﺮﺗ ﻪﺑ ﺎﻫ ﯿ ﺎﺑ ﺮﺑاﺮﺑ ﺐ 512
و 128 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ
دﺮﮔ هﺪﻫﺎﺸﻣ ﯾﺪ
.
Fig 5 Antifungal effect of Ficus benghalensis ethanolic extract, according to the minimum
inhibitory concentration test.
Fig 6 Antifungal effect of Ficus benghalensis ethanolic extract, according to the minimum
fungicidal concentration test.
ﮐﺮﺗ ﻪﮐ ﺖﺳا هﺪﺷ شراﺰﮔ ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ﺎﻧاﻮﺗ ﯾ ﯽ فﺬﺣ
دار ﯾ لﺎﮑ ﺎﻫ ي ﺪﻧراد ار دازآ .
ﻟﺎﻌﻓ ﯿ ﺖ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ
ﻟد ﻪﺑ ًﺎﺗﺪﻤﻋ ﯿ
ﺖﺳا ﺎﻬﻧآ ﺲﮐودر صاﻮﺧ ﻞ هزﺎﺟا ﺎﻬﻧآ ﻪﺑ ﻪﮐ
ﻣ ﯽ ﺣا ﻞﻣاﻮﻋ ناﻮﻨﻋ ﻪﺑ ﺪﻫد ﯿ
ﻫ هﺪﻨﻨﮐ اﺪﻫا ،هﺪﻨﻨﮐ ﺎ ﯿ
و نژورﺪ
هﺪﻨﻨﮐ شﻮﻣﺎﺧ ﺎﻫ
ي ﺴﮐا ﯿ نﮋ ﯾ ﺪﻨﻨﮐ ﻞﻤﻋ ﻪﻧﺎﮕ ]
15 - 17 [ . ا رد ﯾ ﻦ
ﻟﺎﻌﻓ ،ﺎﺘﺳار ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺶﻬﺟ ﺪﺿ و از
ﯾ ﯽ ﺑآ هرﺎﺼﻋ ﯽ
١٧٨
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺗﺎﺳ ﻪﻌﻟﺎﻄﻣ رد ﯿ
نارﺎﮑﻤﻫ و ﺶ )
2013 ( شراﺰﮔ
ﺖﺳا هﺪﺷ ]
9 [ . ﻮﺋار ارﺎﮑﺳﺎﺑ نارﺎﮑﻤﻫ و
) 2014 ( ﻪﮐ داد نﺎﺸﻧ
ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ رﺎﻬﻣ رد
دار ﯾ لﺎﮑ اﻮﺘﺤﻣ و دﻮﺑ ﺖﻈﻠﻏ ﻪﺑ ﻪﺘﺴﺑاو DPPH
ي و لﻮﻨﻓ
ﺋﻮﻧوﻼﻓ ﯿ ﺗﺮﺗ ﻪﺑ ار هرﺎﺼﻋ ﻞﮐ ﺪ ﯿ
ﺐ mg GAE/g 004
/ 53 و
mg QE/g 99
/ 142 ﺪﻧدﻮﻤﻧ شراﺰﮔ ]
18 [ . اﯾ ﻘﻘﺤﻣ ﻦ ﯿ ،ﻦ
ﻨﭽﻤﻫ ﯿ ﺎﺣ هرﺎﺼﻋ ﻪﮐ ﺪﻧداد نﺎﺸﻧ ﻦ و
ي ﮐﺮﺗ ﯿ ﻠﭘ تﺎﺒ ﯽ ﻟﻮﻨﻓ ﯽ
ﻟﺎﮔ ﯿ ﺳا ﮏ ﯿ وﻼﻓﻮﺌﺗ ،ﺪ ﯾ
ﭽﺗﺎﮐ ،ﻦ ﯿ ﻣ نوﻼﻓ و ﻦ ﯽ
ﺪﺷﺎﺑ . رد
ﻪﻌﻟﺎﻄﻣ ا ي د ﯾ اﻮﺘﺤﻣ ،ﺮﮕ ي
ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ﯿ
ﻟﺎﻌﻓ و ﻞﮐ ﺪ ﯿ
ﺖ
ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺴﮐاﺮﻓ و ﯿ نﻮ ﺎﻫ ي
ﺑآ ﯽ ﺗا ، ﯿ د ،تﺎﺘﺳا ﻞ ي
ﻂﺳﻮﺗ نآ لﻮﻧﺎﺗﻮﺑ و ناﺰﮕﻫ ،نﺎﺘﻣوﺮﻠﮐ
نارﺎﮑﻤﻫ و ﻞﺣار )
2017 ( ﻣ ﺳرﺮﺑ درﻮ ﯽ ﺖﻓﺮﮔ راﺮﻗ .
اﻮﺘﺤﻣ ي
ﺗا ﺶﺨﺑ رد لﻮﻨﻓ ﯿ
ﺑ تﺎﺘﺳا ﻞ ﯿ
ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ناﺰ ) mg GAE/g
27 /3 ( ﻟﺎﺣرد ﯽ ﺮﺘﻤﮐ ﻪﮐ ﯾ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ رد نآ ﻦ ﯽ
مﺎﺧ ) mg
GAE/g 004 /0 ( دﻮﺑ . اﻮﺘﺤﻣ ي ﻟﻮﻨﻓ ﯽ هرﺎﺼﻋ رد ﺎﻫ
ي ﻒﻠﺘﺨﻣ
ﺗﺮﺗ ﻪﺑ ﯿ ﺗا ﺐ ﯿ تﺎﺘﺳا ﻞ ﺑآ <
ﯽ د <
ي نﺎﺘﻣوﺮﻠﮐ
<
-n ناﺰﮕﻫ
<
-n
لﻮﻧﺎﺗﻮﺑ ﺶﻫﺎﮐ لﻮﻧﺎﺘﻣ <
ﯾ ﺖﻓﺎ . ﺤﻣ اﻮﺘ ي ﺋﻮﻧوﻼﻓ ﯿ رد ﻞﮐﺪ
ﺴﮐاﺮﻓ ﯿ ﺑآ نﻮ ﯽ ) mg GAE/g 51
/0 ( ﺮﺗﻻﺎﺑ ﯾ رد و ﻦ
ﺴﮐاﺮﻓ ﯿ نﻮ ﺎﻫ ي -n د و ناﺰﮕﻫ ي
نﺎﺘﻣوﺮﻠﮐ )
mg GAE/g
001 /0 ( ﺮﺘﻤﮐ ﯾ دﻮﺑ ﻦ . ﻣﯿ ﺋﻮﻧوﻼﻓ ناﺰ ﯿ
ﺎﻫﺪ ي هرﺎﺼﻋ رد ﻞﮐ ﺎﻫ
ي
ﺗﺮﺗ ﻪﺑ ﻒﻠﺘﺨﻣ ﯿ
ﺑآ ﺐ ﯽ ﺗا <
ﯿ تﺎﺘﺳا ﻞ
<
-n لﻮﻧﺎﺗﻮﺑ مﺎﺧ <
د <
ي
نﺎﺘﻣوﺮﻠﮐ
<
-n ﺶﻫﺎﮐ ناﺰﮕﻫ ﯾ
ﺖﻓﺎ . ﺮﺗﻻﺎﺑ ﯾ ﻟﺎﻌﻓ ﻦ ﯿ ﻣ ﺖ رﺎﻬ
دار ﯾ ﺑ رد لﺎﮑ ﯿ هرﺎﺼﻋ مﺎﻤﺗ ﻦ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﻪﺑ طﻮﺑﺮﻣ ﺎﻫ
ﯽ مﺎﺧ
ﺑﯿ ﻦ 77 / 71 ﺎﺗ 6/
82 دﻮﺑ ﺪﺻرد ]
19 [ . ﮐﺮﺗ ﯿ ﻠﭘ تﺎﺒ ﯽ ﻟﻮﻨﻓ ﯽ ﺪﻨﻧﺎﻣ
ﺋﻮﻧوﻼﻓ ﯿ ﺳا ،ﺎﻫﺪ ﯿ ﺎﻫﺪ ي ﻟﻮﻨﻓ ﯿ ﻦﻧﺎﺗ و ﮏ ﻠﺻا ﻞﻣاﻮﻋ ناﻮﻨﻋ ﻪﺑ ﺎﻫ ﯽ
ﻟﺎﻌﻓ رد ﯿ ﺖ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﮔ ﯿ وراد نﺎﻫﺎ ﯾ ﯽ
، ﻣﯿ هﻮ ﺰﺒﺳ و ﺎﻫ ي رد ﺎﻫ
ﻣ ﻪﺘﻓﺮﮔ ﺮﻈﻧ ﯽ
ﺪﻧﻮﺷ ] 20 [.
ا ﺎﺑ ﯾ ﺑ توﺎﻔﺗ ،لﺎﺤﻨ ﯿ
ﺎﺘﻧ ﻦ ﯾ ا رد ﺞ ﯾ ﻦ
ﻠﺒﻗ تﺎﻌﻟﺎﻄﻣ ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﯽ
ﺷﺎﻧ ًﺎﺗﺪﻤﻋ ﯽ ا زا ﯾ ﻘﺣ ﻦ ﯿ ﻪﮐ ﺖﺳا ﺖﻘ
ﮔ ﺪﺷر ﯿ ﮐﺮﺗ و هﺎ ﯿ ﺐ / ﻟﺎﻌﻓ ﯿ ﺑ ﺖ ﯿ ژﻮﻟﻮ ﯾﮑ ﯽ تﺪﺷ ﻪﺑ نآ هرﺎﺼﻋ
ﺛﺄﺗ ﺖﺤﺗ ﯿ ﻔﻠﺘﺨﻣ ﻞﻣاﻮﻋ ﺮ ﯽ
ﺒﻗ زا ﯿ ﺘﻧژ ﻞ ﯿ ،ﺎﻣد ،ﺪﺷر ﻪﻠﺣﺮﻣ ،ﮏ
اﺮﺷ و ﺖﺑﻮﻃر ﯾ
هرﺎﺼﻋ جاﺮﺨﺘﺳا ﻂ دراد راﺮﻗ
] 21 , 22 [.
ﻪﻌﻟﺎﻄﻣ ي ﺮﺳ ﯾ اﻮ نارﺎﮑﻤﻫ و اوﺎﺘﺳ )
2022 ( هرﺎﺼﻋ ﻪﮐ داد نﺎﺸﻧ
ﺑآ ﯽ ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺪﺷر رﺎﻬﻣ ﻪﺑ ردﺎﻗ ﺪﻧﺎﮐ
ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ اﺮﺷ رد ﯾ ﻂ
ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ ] 23 [ . نارﺎﮑﻤﻫ و ﻮﮑﻨﭼﺎﺗ )
2017 ( ﻟﺎﻌﻓ ، ﯿ ﺖ
ﻣ ﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﻬﺗ ﯿ ﺠﻧا گﺮﺑ زا هﺪﺷ ﻪ ﯿ
ﻟﺎﮕﻨﺑ ﺮ ﯽ رد
ﺮﺘﮐﺎﺑ ﺮﺑاﺮﺑ ي
ﺎﻫ ي ﺖﺒﺜﻣ مﺮﮔ ﻓﺎﺘﺳا)
ﯿ وا سﻮﮐﻮﮐﻮﻠ سﻮﺋر
و
ﻧﻮﻣﻮﻨﭘ سﻮﮐﻮﮐﻮﺘﭘﺮﺘﺳا ﯿﻪ
(،
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ سﺎﻧﻮﻣودﻮﺳ) ژوﺮﺋا
ﯾ ازﻮﻨ ﺴﺒﻠﮐ ، ﯿ ﻧﻮﻣﻮﻨﭘ ﻼ ﯿﻪ ﺷﺮﺷا و ﯿ ﻠﮐﺎ ﯽ ( چرﺎﻗ و ﺪﻧﺎﮐ ﯾ اﺪ
ﺒﻟآ ﯿ ﺲﻨﮑ زرا ار ﯾﺑﺎ ﯽ ﺪﻧدﻮﻤﻧ . ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﻟﺎﻌﻓ ﯿ ﺪﺿ ﺖ
ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﻄﺳﻮﺘﻣ ﯽ ﺮﺑاﺮﺑ رد ار ﻓﺎﺘﺳا
ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ،
ﺷﺮﺷا ﯿ ﻠﮐ ﺎ ﯽ ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ و ﯾ
ازﻮﻨ ﺎﺸﻧ ﻟﺎﺣرد ،داد ن ﯽ
ﻪﮐ
ﻟﺎﻌﻓ ﯿ ﺮﺘﮐﺎﺑ ﺪﺿ ﺖ ﯾﺎﯾ
ﯿ ﻨﻌﻤ ﯽ راد ي ﺮﺑاﺮﺑ رد ﺴﺒﻠﮐ ﯿ ﻧﻮﻣﻮﻨﭘ ﻼ ﯿﻪ و
ﻧﻮﻣﻮﻨﭘ سﻮﮐﻮﮐﻮﺘﭘﺮﺘﺳا ﯿﻪ
ﺪﻧﺎﮐو ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ داﺪﻧ نﺎﺸﻧ . ﻣ رد ﯿ نﺎ
ﻮﺳ ﯾﻪ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ﺎﻣزآ ﯾ ﺶ ﺮﺘﮐﺎﺑ ،هﺪﺷ ي
چرﺎﻗ ﻪﺑ ﺖﺒﺴﻧ ﺎﻫ ﺎﻫ
سﺎﺴﺣ ﻟﺎﻌﻓ و ﺪﻧدﻮﺑ ﺮﺗ ﯿ
ﺮﺘﮐﺎﺑ ﺪﺿ ﺖ ﯾﺎﯾ
ﯽ ور ﯾ ﺮﺘﮐﺎﺒ ي ﺎﻫ ي مﺮﮔ
ﮐﺎﺑ ﻪﺑ ﺖﺒﺴﻧ ﺖﺒﺜﻣ ﺮﺘ
ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ دﻮﺑ ﺮﺗزرﺎﺑ .
ﻟﺎﻌﻓ ﯿ ﺖ ﺎﻫ ي
ﺮﺘﮐﺎﺑ ﺪﺿ ﯾﺎﯾ ﯽ ا هدﺮﺘﺴﮔ ﯾ ﻟﻮﺑﺎﺘﻣ ﻪﺑ هرﺎﺼﻋ ﻦ ﯿ
ﺖ ﺎﻫ ي ﻮﻧﺎﺛ ﯾ ﻪ
ﮔﯿ ﻫﻮﺑﺮﮐ ﺪﻨﻧﺎﻤﻫﺎ ﯿ
تارﺪ ﺎﻫﺪﻨﻗ ،ﺎﻫ ي
ﺣا ﯿ لوﺮﺘﺳا ،هﺪﻨﻨﮐﺎ ،ﺎﻫ
ﻠﮔ ﯿ زﻮﮑ ﯾ ﮐﺮﺗ ،ﺎﻫﺪ ﯿ تﺎﺒ ﻟﻮﻨﻓ ﯽ ﻦﻧﺎﺗ ، ﻧﻮﭘﺎﺳ ،ﺎﻫ ﯿ ﻦ ﺋﻮﻧوﻼﻓ و ﺎﻫ ﯿ
ﺎﻫﺪ
ﺪﺷ هداد ﺖﺒﺴﻧ ]
24 [ . ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
گﺮﺑ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﺴﮐاﺮﻓ و ﯿ نﻮ ﺎﻫ ي چرﺎﻗ ﺮﺑاﺮﺑ رد نآ ﺪﻧﺎﮐ
ﯾ اﺪ
ﺒﻟآ ﯿ ﺲﻨﮑ داوﺎﮔﻻآ و زاﻮﻧﺎﺑ ﻪﻌﻟﺎﻄﻣ رد ي
) 2016 ( هﺪﺷ شراﺰﮔ
ﺖﺳا ] 25 [.
ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﺮﺗﻻﺎﺑ ﯽ
ﺠﻧا ﯿ ﺮ
ﻟﺎﮕﻨﺑ ﯽ ﺎﻘﻣ رد رﺎﮔآ ﮏﻫﺎﭼ شور رد ﯾ
د شور ﺎﺑ ﻪﺴ ﯾ
ﮏﺴ
دﯾ ﻔﯿ ﺷﺎﻧ ﺖﺳا ﻦﮑﻤﻣ رﺎﮔآ نژﻮ ﯽ
ﻘﺘﺴﻣ سﺎﻤﺗ زا ﯿ
ﻢ ﺎﺼﻋ ﺎﺑ هر
ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ا رد ﺎﻫ ﯾ ﺪﺷﺎﺑ شور ﻦ .
ﻟﺎﺣرد ﯽ ﻪﮐ ﺪﺿ نﻮﻣزآ رد
ﻣﯿ ﺑوﺮﮑ ﯿ ﺪﯾ د ﮏﺴ ﯾﻔ ﯿ ﺎﺑ هرﺎﺼﻋ ،رﺎﮔآ نژﻮ ﯾ
ﺘﺴ ﯽ د حﻮﻄﺳ زا ﯾ
ﮏﺴ
ﺤﻣ ﻞﺧاد ﻪﺑ ﯿ
ﻂ رﺎﺸﺘﻧا ﯾ ﮔﺪﻧرادزﺎﺑ ﺮﺛا ﺎﺗ ﺪﺑﺎ ﯽ
ﺪﻫد نﺎﺸﻧ ار دﻮﺧ
] 13 , 14 , 21 [.
ﻤﺘﺣر ﯽ ﻨﺟ ﯿ ﻠﻋ و دﺎﺑآﺪ ﯿ
ﻧﺎﻬﺒﻬﺑ هداﺰ ﯽ ) 2021 (
ﻟﺎﻌﻓ ﻪﮐ ﺪﻧداد نﺎﺸﻧ ﯿ
ﺑ ﺖ ﯿ ژﻮﻟﻮ ﯾ ﮑ ﯽ ﺲﻧﺎﺳا و هرﺎﺼﻋ ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ
ﺷﺎﻧ ﯽ ﮐﺮﺗ رﻮﻀﺣ زا ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻟﺎﻌﻓ ﻪﮐ ﺖﺳا ﺎﻬﻧآ رد ﯿ
ﺪﺿ ﺖ
ﻣﯿ ﺑوﺮﮑ ﯿ ﻟد ﻪﺑ ار دﻮﺨ ﯿ
ﺗﺎﻣورآ ﻪﺘﺴﻫ دﻮﺟو ﻞ ﯿ
هوﺮﮔ و ﮏ OH
ﻟﻮﻨﻓ ﯿ ﮑﺸﺗ ﻪﺑ ردﺎﻗ و هداد زوﺮﺒ ﯿ
ﭘ ﻞ ﯿ ﺎﻫﺪﻧﻮ ي ﻫ ﯿ ﻧژورﺪ ﯽ ﺎﺑ
هوﺮﮔ ﺎﻫ ي نﺎﮑﻣ رد -SH ﺎﻫ ي ﺰﻧآ لﺎﻌﻓ ﯾ ﻢ ﺎﻫ ي ﺘﻧ رد و فﺪﻫ ﯿ
ﻪﺠ
ﻏﯿ زﺎﺳ لﺎﻌﻓﺮ ي
ﺰﻧآ ﯾ ﻢ ﻫﺎ ي ﭼرﺎﻗ ﯽ ﻣ ﯽ ﺪﻨﺷﺎﺑ . ا ﺮﺑ هوﻼﻋ ﯾ
،ﻦ
ﻫﺎﻣ ﯿ ﺮﮕﺑآ ﺖ ﯾ ﮐﺮﺗ ﺰ ﯿ هرﺎﺼﻋ تﺎﺒ ﺲﻧﺎﺳا و ﺎﻫ
ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ ﺐﺒﺳ
ﻣ ﻪﺑ ﺎﻬﻧآ نﺪﺷ بﺬﺟ ﯿ
ﻠﺴ ﯿ ﭼرﺎﻗ مﻮ ﯽ ﮔﻮﻠﺟ و ﯿﺮ ي نآ ﺪﺷر زا
ﻣ ﯽ ددﺮﮔ ] 26 , 27 [ . ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ ا ﺞ ﯾ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ،ﻪﻌﻟﺎﻄﻣ ﻦ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ﮔﻮﻠﺟ ﻪﺑ ردﺎﻗ ﯿﺮ
ي نژﻮﺗﺎﭘ ﺪﺷر زا ﺎﻫ
ي ﭼرﺎﻗ ﯽ
ﻣﯿ هﻮ ﺰﺒﺳ و ﺎﻫ ي ﻣﺎﻫ ﯽ ﻣ و ﺪﺷﺎﺑ ﯽ ﮐﺮﺗ ناﻮﻨﻌﺑ ﺪﻧاﻮﺗ ﯿﺒ
ﯽ ﺒﻃ ﯿﻌ ﯽ
اﺰﻓا ﺖﻬﺟ ﯾ راﺪﻬﮕﻧ ﺮﻤﻋ ﺶ ي
اﺬﻏ تﻻﻮﺼﺤﻣ ﯾ
ﯽ ددﺮﮔ هدﺎﻔﺘﺳا .
4 - ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي
ﻟد ﻪﺑ ﯿ ﻞ اﻮﺘﺤﻣ ي ﻟﻮﻨﻓ ﯽ ﺋﻮﻧوﻼﻓ و ﯿ ﺪ ي ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ،ﻻﺎﺑ ﯽ
ﺠﻧا ﯿ ﺮ
١٧٩
ﻟﺎﮕﻨﺑ ﯽ ﻟﺎﻌﻓ ﯿ ﺖ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻞﺑﺎﻗ ﻬﺟﻮﺗ ﯽ ﺖﺷاد . ا ﺮﺑ هوﻼﻋ ﯾ
،ﻦ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ ا ﻣ ﺪﺿ ﺮﺛ ﯿ ﺑوﺮﮑ ﯽ ﻞﺑﺎﻗ ﻬﺟﻮﺗ ﯽ ﺮﺑ
ور ي ﻮﺳ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ نژﻮﺗﺎﭘ ﻣ دﺎﺴﻓ ﻞﻣﺎﻋ ﯿ
ﻃ لﺎﻘﺗﺮﭘ هﻮ ﯽ
ﻧﺎﻣرﺎﺒﻧا ﯽ ﻨﭘ ) ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ( نﺎﺸﻧ
داد . ﺎﺘﻧ ﯾ ﻣ نﺎﺸﻧ ﺞ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ ﺪﻫد ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯿﻤ ﯽ ﺪﻧاﻮﺗ
ﻤﻬﻣ ﻊﺒﻨﻣ ﯽ ﮐﺮﺗ زا ﯿ تﺎﺒ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣ ﺪﺿ و ﯿ ﺑوﺮﮑ ﯽ و ﺪﺷﺎﺑ
اﺮﺑ ﺖﺳا ﻦﮑﻤﻣ ي
اﺰﻓا ﯾ رﺎﮔﺪﻧﺎﻣ ﺮﻤﻋ ﺶ ي
ﺴﺑ ﯿ رﺎ ي تﻻﻮﺼﺤﻣ زا
اﺬﻏ ﯾ ﯽ زروﺎﺸﮐ و ي
دور رﺎﮑﺑ . و رﻮﻄﺑ ﯾ ﭘ ،هﮋ ﯿ ﻣ دﺎﻬﻨﺸ ﯽ ﻪﮐ دﻮﺷ
ﺘﻧآ ﺮﺛا ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﭼرﺎﻗ ﺪﺿ و ﯽ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ
ﺠﻧا ﯿ ﻟﺎﮕﻨﺑ ﺮ ﯽ
ور ي ﻣ ﯿ ﻃ و لﺎﻘﺗﺮﭘ هﻮ ﯽ
نﺎﻣز / ﺎﻫﺎﻣد ي راﺪﻬﮕﻧ ﻒﻠﺘﺨﻣ ي
ﺳرﺮﺑ ﯽ
ددﺮﮔ .
5 - ﺪﻘﺗ ﯾ ﺮﮑﺸﺗ و ﺮ
جﺮﺨﺘﺴﻣ ﺮﺿﺎﺣ ﻪﻟﺎﻘﻣ ﺸﻫوﮋﭘ حﺮﻃ زا
ﯽ ﺪﮐ ﺎﺑ 28 / 1401
ﻣ ﯽ ﻣ مزﻻ دﻮﺧ ﺮﺑ ﻪﻟﺎﻘﻣ نﺎﮔﺪﻨﺴﯾﻮﻧ اﺬﻟ ،ﺪﺷﺎﺑ ﯽ
ﺖﻧوﺎﻌﻣ زا ﺪﻨﻧاد
ﺸﻫوﮋﭘ ﯽ روﺎﻨﻓ و ي زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ
ﯽ
ﻟد ﻪﺑ نﺎﺘﺳزﻮﺧ ﯿ
ﺎﻤﺣ ﻞ ﯾ ﺖ ﺎﻫ ي دﺎﻣ ي ﻮﻨﻌﻣ و ي ﻤﺻ ﯿ ﺮﮑﺸﺗ ﻪﻧﺎﻤ
ﻧادرﺪﻗ و ﯽ ﺪﻨﯾﺎﻤﻧ .
6 - ﻊﺑﺎﻨﻣ
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١٨١
Evaluation of total phenol and flavonoids, radical scavenging ability and antifungal effect of Ficus benghalensis ethanolic extract on fungi species causing rot in orange fruit during storage
Rahmati-Joneidabad, M. 1*, Alizadeh Behbahani, A. 2, Noshad, M. 3
1. Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2. Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3. Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
ABSTRACT ARTICLE INFO
Article History:
Received 2022/ 11/ 08 Accepted 2022/ 12/ 12
Keywords:
Orange,
Ficus benghalensis, Bioactive extract, Penicillium digitatum, Penicillium italicum. The aim of this study was to investigate the phytochemical analysis and
antifungal activities of ethanolic extract of Ficus benghalensis on the growth of fungal strains causing rot in orange fruit during storage (Penicillium digitatum and Penicillium italicum). Total phenol content was evaluated according to Folin-Ciocalteu method, total flavonoid content was evaluated according to aluminum chloride colorimetric method, and antioxidant activity was evaluated based on DPPH and ABTS free radical scavenging methods. Various methods (disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration) were used to evaluate the antifungal activity of ethanolic extract of F. benghalensis. The amount of phenol and flavonoids in the whole extract was 110.49 mg GAE/g and 62.60 mg QE/g, respectively.
Antioxidant activity of ethanolic extract of F. benghalensis based on DPPH and ABTS radical inhibition methods was 48.56 and 57.20 μg/ml, respectively. The results of disk diffusion agar and well diffusion agar tests showed that the antifungal activity of the extract was concentration dependent and the fungal strains of Penicillium digitatum and Penicillium italicum with the lowest and highest diameter of growth inhibition zone, were the most resistant and sensitive species to the extract, respectively. The minimum inhibitory concentrations for the above strains were 16 and 8 mg/ml, respectively, and the minimum fungicidal concentrations were 512 and 128 mg/ml, respectively. According to the results, ethanolic extract of F. benghalensis is an important source of antioxidant and antifungal compounds and can be used to increase the shelf life of horticulturalproducts.
DOI: 10.22034/FSCT.19.132.173
DOR: 20.1001.1.20088787.1401.19.132.13.6
*Corresponding Author E-Mail:
Journal of Food Science and Technology (Iran)
Homepage:www.fsct.modares.ir Scientific Research
