ﻣﺠﻠﻪ ﻋﻠﻮم و ﺻﻨﺎ ﻊﯾ ﻏﺬا ﯽﯾ ﯾا ﺮان

(1)

ي ﺎﻫ ﯽ ﮔﮋ ﯾ و ﯽ ﺳرﺮﺑ ﺷ

ﯿﻤ ﯿﺎ ﯾ ﯽ ﻟﺎﻌﻓ و ﯿ ﻣﺪﺿ ﺖ ﯿ

ﺑوﺮﮑ ﯽ ﺮﺑ كزار هرﺎﺼﻋ Staphylococcus aureus

،

Listeria monocytogenes

، Escherichia coli و

Enterobacter aerogenes اﺮﺷ رد

ﯾ ﻂ

نوﺮﺑ ﻨﺗ ﯽ

دﺎﺷﻮﻧ ﺪﻤﺤﻣ

1

ﻠﻋ زوﺮﻬﺑ ،*

ﯿ ﻧﺎﻬﺒﻬﺑ هداﺰ

2ﯽ ﻔﻄﺼﻣ ، ﯽ ﺘﻤﺣر ﯽ ﻨﺟ ﯿ دﺎﺑآﺪ 3

1 - ﺸﻧاد ﯿ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ

ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻠﻋ هﺪﮑﺸﻧاد ، ﻣاد مﻮ

ﯽ ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي

ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ ﯽ

ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ

ا ، ﯾ ناﺮ .

2 - دﺎﺘﺳا ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ

ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ، ﯽ

ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي

ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ ﯽ

ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ

ا ، ﯾ ناﺮ .

3 - دﺎﺘﺳا ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ

ﺎﺒﻏﺎﺑ ﻧ ﯽ زروﺎﺸﮐ هﺪﮑﺸﻧاد ، ي

زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي

ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ ﯽ ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ

ا ، ﯾ ناﺮ .

ﻪﻟﺎﻘﻣ تﺎﻋﻼﻃا ﮑﭼ

ﯿ هﺪ

رﺎﺗ ﯾ ﺎﻫ ﺦ ي ﻪﻟﺎﻘﻣ :

رﺎﺗ ﯾ رد ﺦ ﯾ ﺖﻓﺎ : 03 / 10 / 1400

رﺎﺗ ﯾ ﺬﭘ ﺦ ﯾ شﺮ : 03 / 12 / 1400

ﻠﮐ تﺎﻤﻠﮐ ﯿ ﺪ ي :

كزار هرﺎﺼﻋ

،

ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ

،

ﻣ ﺪﺿ ﯿ بوﺮﮑ

،

ﺮﺘﮐﺎﺑ ي نژﻮﺗﺎﭘ

،

ﺒﻃ هﺪﻧراﺪﻬﮕﻧ ﯿﻌ

ﯽ .

DOI: 10.22034/FSCT.19.126.143

DOR: 20.1001.1.20088787.1401.19.126.15.6

تﺎﺒﺗﺎﮑﻣ لﻮﺌﺴﻣ 

: [email protected]

ا رد ﯾ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ،ﻪﻌﻟﺎﻄﻣ ﻦ ﯽ

ﮔ ﯿ ﺧ شور زا هدﺎﻔﺘﺳا ﺎﺑ كزار هﺎ ﯿ

ﻬﺗ نﺪﻧﺎﺴ ﯿ ﺪﺷ ﻪ . ﻟﺎﻌﻓ ﯿ ﺖ

ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﺮﺘﮐﺎﺑ ﺮﺑاﺮﺑ رد نآ ي

ﺎﻫ ي نژﻮﺗﺎﭘ ) Staphylococcus aureus

، Listeria

monocytogenes

، Escherichia coli و

Enterobacter aerogenes (

ﻖﺑﺎﻄﻣ

شور ﺎﻫ ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ،رﺎﮔآ نژﻮ ﯽ

ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و

ﮔﺪﻨﺸﮐ ﯽ ﺳرﺮﺑ ﯽ دﺮﮔ ﯾ ﺪ . شور زا ﺠﻨﺳ ﮓﻧر ﯽ

ﻟﻮﻓ فﺮﻌﻣ ﯿ

ﻦ - ﺳﯿ ﺮﻠﮐ و ﻮﺘﻟﺎﮐﻮ ﯾ

ﻣﻮﻟآ ﺪ ﯿﻨﯿ مﻮ

هزاﺪﻧا ﺖﻬﺟ ﮔﯿ

ﺮ ي اﻮﺘﺤﻣ ي ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ﯿ

ﺪﺷ هدﺎﻔﺘﺳا هرﺎﺼﻋ ﻞﮐ ﺪ .

ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ

ﻧ هرﺎﺼﻋ ﯿ شور ﺎﺑ ﺰ ﺎﻫ ي دار رﺎﻬﻣ ﯾ دازآ لﺎﮑ وDPPH

ﺳرﺮﺑABTS ﯽ دﺮﮔ ﯾ ﺪ .ﺑ ﯿ ﺮﺘﺸ ﯾ و ﻦ

ﺮﺘﻤﮐ ﯾ شور رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦ ﺎﻫ

ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ نژﻮ ) 9 / 16 ﻣ ﯿﻠ ﯽ ﺮﺑاﺮﺑ رد ﺮﺘﻣ 5

/ 12

ﻣﯿ ﻠ ﯽ ﺮﺘﻣ ( رﺎﮔآ ﮏﻫﺎﭼ و )

6 / 17 ﻣ ﯿﻠ ﯽ ﺮﺑاﺮﺑ رد ﺮﺘﻣ 9

/ 13 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ﺗﺮﺗ ﻪﺑ ﯿ ﺮﺘﮐﺎﺑ رد ﺐ ي

ﺎﻫ ي S.

aureus و E. aerogenes ﺪﺷ هﺪﻫﺎﺸﻣ

. ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔ عاﻮﻧا ﻪﺑ ﺖﺒﺴﻧ ﺖﺒﺜﻣ مﺮﮔ ﯽ

رد

ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﺮﺑاﺮﺑ ﯽ

ﮔ ﯿ سﺎﺴﺣ كزار هﺎ ﺪﻧدﻮﺑ ﺮﺗ

. اﻮﺘﺤﻣ ي ﻮﻧوﻼﻓ و لﻮﻨﻓ ﺋﯿ

ﻪﺑ هرﺎﺼﻋ ﻞﮐ ﺪ

ﺗﺮﺗ ﯿ ﺎﺑ ﺮﺑاﺮﺑ ﺐ mg GAE/g

47 / 96 و mg QE/g 5

/ 28 دﻮﺑ . ﺘﻧآ ﺮﺛا ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ هرﺎﺼﻋ

ﻟﻮﻧﺎﺗا ﯽ شور سﺎﺳا ﺮﺑ كزار ﺎﻫ

ي دار رﺎﻬﻣ ﯾ دازآ لﺎﮑ وDPPH

ﺗﺮﺗ ﻪﺑABTS ﯿ ﺐ 63 / 58 و

5 / 66 هزاﺪﻧا ﺪﺻرد ﮔﯿ

ﺮ ي دﺮﮔ ﯾ ﺪ . ﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯾ

ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ،ﺞ ﯽ

ﮔ ﯿ ﻣ ار كزار هﺎ ﯽ

ناﻮﺗ

ﮐﺮﺗ ناﻮﻨﻌﺑ ﯿ

ﻣ ﺪﺿ ﺐ ﯿ ﺘﻧآ و بوﺮﮑ ﯽ

ﺴﮐا ﯿ ﺒﻃ ناﺪ ﯿﻌ ﯽ ﮔﻮﻠﺟ ﺖﻬﺟ ﯿﺮ

ي ﺮﺘﮐﺎﺑ ﺪﺷر زا ي

ﺎﻫ ي

ﺴﮐا شﺮﺘﺴﮔ و نژﻮﺗﺎﭘ ﯿ

ﺳاﺪ ﯿ ﻟ نﻮ ﯿﭙﯿ اﺬﻏ تﻻﻮﺼﺤﻣ عاﻮﻧا رد ﺪ ﯾ

ﯽ دﻮﻤﻧ هدﺎﻔﺘﺳا .

ﻪﻠﺠﻣ

مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ

www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ

ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ

[ DOR: 20.1001.1.20088787.1401.19.126.15.6 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-06 ]

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1 - ﻪﻣﺪﻘﻣ

ﭘ ﺎﺑ ﯿ ﻟﻮﺗ رد ﺖﻓﺮﺸ ﯿ

ﺎﻫوراد ﺪ ي ﺷ ﯿﻤ ﯿﺎﯾ ﯽ ﺪﺟ ﯾ ﺘﻧآ و ﺪ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ -

ﺎﻫ ي رﺪﺗ ﻪﺑ ،توﺎﻔﺘﻣ ﯾ

ﺞ ا ﺮﻀﻣ تاﺮﺛا ﯾ

ﺖﻣوﺎﻘﻣ و ﺎﻫوراد ﻦ

ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮑ ﯽ اﺮﻓا ﯾ ﺶ ﯾ ا ﻪﮐ ﺖﺳا ﻪﺘﻓﺎ ﯾ

اﺰﻓا ﻪﺑ ﺮﺠﻨﻣ ﺮﻣا ﻦ ﯾ

ﺶ

ﺎﻤﺗ ﯾ ﮔ زا هدﺎﻔﺘﺳا ﻪﺑ ﻞ ﯿ

وراد نﺎﻫﺎ ﯾ ﯽ ﺖﺳا هﺪﺷ .

ﺎﻫوراد ي ﺒﻃ ﯿﻌ ﯽ

ﮔ و ﯿ وراد نﺎﻫﺎ ﯾ ﯽ ﻪﺑ نﺎﻣرد ﺖﻬﺟ هدﺎﻔﺘﺳا درﻮﻣ داﻮﻣ ﻪﻠﻤﺟ زا

ﻣ رﺎﻤﺷ ﯽ آﯾ ﻟوا داﻮﻣ و ﺪﻨ ﯿ

نآ ﻪ زﺎﺳوراد ﺖﻌﻨﺻ رد ﺎﻫ ي

درﻮﻣ

ﻔﺘﺳا ﻣ راﺮﻗ هدﺎ ﯽ ﮔﯿ ﺎﺟ و دﺮ ﯾ ﺰﮕ ﯾ ﺒﺳﺎﻨﻣ ﻦ ﯽ اﺮﺑ ي ﺎﻫوراد ي ﺘﻌﻨﺻ ﯽ

ﻣ رﺎﻤﺷ ﻪﺑ ﯽ

آﯾ ﺪﻨ ] 1 [ . هرﺎﺼﻋ دﺮﺑرﺎﮐ سﺎﺳا ﺎﻫ

ي ﮔ ﯿ ﻫﺎ ﯽ رد

راﺪﻬﮕﻧ ي اﺬﻏ داﻮﻣ ﯾ ﯽ وراد ، ﯾ ﯽ ارآ و ﯾ ﺸ ﯽ ﻟد ﻪﺑ ﯿ صاﻮﺧ ﻞ

دﺮﮑﻠﻤﻋ ي ﻟﺎﻌﻓ ﺪﻨﻧﺎﻣ ﺎﻬﻧآ ﯿ

ﻣ ﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﺘﻧآ و ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣ ﯽ -

ا ﻪﮐ ﺪﺷﺎﺑ ﯾ ﻟد ﻪﺑ ﺮﻣا ﻦ ﯿ

ﻃ دﻮﺟو ﻞ ﯿ

ﺳو ﻒ ﯿﻌ ﯽ ﮐﺮﺗ زا ﯿ لﺎﻌﻓ تﺎﺒ

ﺑﯿ ژﻮﻟﻮ ﯾﮑ ﯽ نآ رد ﺖﺳا هﺪﺷ شراﺰﮔ ﺎﻫ ]2

[ . هرﺎﺼﻋ ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ

اراد ي ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯿ ﺳا ﺪﻨﻧﺎﻣ ﻒﻠﺘﺨﻣ ﮏ ﯿ

ﺎﻫﺪ ي ﻟﻮﻨﻓ ﯽ ﻦﻧﺎﺗ ، و ﺎﻫ

ﺋﻮﻧوﻼﻓ ﯿ ﮐﺮﺗ ،ﺎﻫﺪ ﯿ ﻧ تﺎﺒ ﯿ ﻧژوﺮﺘ ﯽ ﻣآ ﻪﻠﻤﺟ زا ﯿ

ﺳاﻮﻨ ﯿ تﺎﻘﺘﺸﻣ ،ﺎﻫﺪ

ﻓوﺮﻠﮐ ﯿ ﺋﻮﻟﺎﮑﻟآ ،ﻞ ﯿ ﺳا ،ﺎﻫﺪ ﯿ ﺑرﻮﮑﺳآ ﺪ ﯿ ﺋﻮﻨﺗورﺎﮐ و ﮏ ﯿ

ﻣ ﺪ ﯽ ﺪﻨﺷﺎﺑ .

هرﺎﺼﻋ ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ ﻪﺑ ﻟد ﯿ ﮐﺮﺗ دﻮﺟو ﻞ ﯿ

ا ،ﻢﻬﻣ تﺎﺒ ﯾ

ﺎﺑ و ندﻮﺑ ﻦﻤ

وﯾ ﮔﮋ ﯽ ﺎﻫ ي ﺑ ﯿ ژﻮﻟﻮ ﯾﮑ ﯽ ﻟﺎﻌﻓ ﺪﻨﻧﺎﻣ ﻪﻠﻤﺟ زا ﻒﻠﺘﺨﻣ ﯿ

ﺪﺿ ﺖ

ﻣﯿ ﺑوﺮﮑ ﯽ ﺑﺎﻬﺘﻟاﺪﺿ ، ﯽ ﺘﻧآو ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻪﺘﻓﺮﮔ راﺮﻗ ﻪﺟﻮﺗ درﻮﻣ ﺪﻧا

] 3 [ . ﻣ ﺪﺿ تاﺮﺛا ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ رد ﯿ

ﺑوﺮﮑ ﯽ ﺘﻧآ و ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ

هرﺎﺼﻋ ﮔ ﯿ ﺖﺳا هﺪﺷ شراﺰﮔ ﻒﻠﺘﺨﻣ نﺎﻫﺎ .

نارﺎﮑﻤﻫ و ﺮﮔزﺮﺑ

] 4 [ ﺳرﺮﺑ ﺎﺑ ﯽ ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﺋﻮﻧوﻼﻓ و ﯿ ﺪ ي ﺑآ هرﺎﺼﻋ ﯽ و ﺎﻨﺣ

ﺪﺿ صاﻮﺧ ﻣ

ﯿ ﺑوﺮﮑ ﯽ ﺘﻧآ و ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ا ﯾ ﮔ ﻦ ﯿ ناﻮﻨﻋ ﻪﺑ ار نآ ،هﺎ

ﯾ ﮐﺮﺗ ﮏ ﯿ ز ﺐ ﯾ ﺒﻃ لﺎﻌﻓ ﺖﺴ ﯿﻌ

ﯽ ﻓﺮﻌﻣ ﯽ ﺪﻧدﺮﮐ . هﺪﺷ شراﺰﮔ

ﺖﻈﻠﻏ ﻪﮐ ﺖﺳا ﺎﻫ

ي هرﺎﺼﻋ ﻒﻠﺘﺨﻣ ﻟﻮﻧﺎﺗا

ﯽ ﮔ ﯿ هرﻮﭙﻠﮐ هﺎ

) Teucrium polium (

اراد ي ﻣ ﺪﺿ ﺮﺛا ﯿ

ﺑوﺮﮑ ﯽ ﺑاﺮﺑ رد ﺮ

ﺴﺑ ﯿ رﺎ ي ﺮﺘﮐﺎﺑ زا ي ﺎﻫ ي اﺬﻏ داﻮﻣ دﺎﺴﻓ ﻞﻣﺎﻋ ﯾ

ﯽ ﻣ ﯽ ﺪﺷﺎﺑ ]5 [ .

ﻨﭽﻤﻫ ﯿ هرﺎﺼﻋ ﻪﮐ ﺖﺳا هﺪﺷ شراﺰﮔ ﻦ ﺎﻫ

ي ﺑآ ﯽ ﻟﻮﻧﺎﺗا و ﯽ گﺮﺑ

ﻟﺎﮐا ﯿ اراد سﻮﺘﭙ ي ﻣ ﺪﺿ ﺮﺛا ﯿ ﺑوﺮﮑ ﯽ ﻻﺎﺑ ﯾ ﯽ ﺮﺘﮐﺎﺑ ﺮﺑاﺮﺑ رد ي

ﺎﻫ ي

ﻣ ﺖﺒﺜﻣ مﺮﮔ ﯽ

ﺪﺷﺎﺑ ]6 [.

ﮔﯿ ﻤﻠﻋ مﺎﻧ ﺎﺑ كزار هﺎ ﯽ

Humulus lupulus هداﻮﻧﺎﺧ زا

ﻪﻧاﺪﻫﺎﺷ ) Cannebinaceae (

ﻣ ﯽ ﺪﺷﺎﺑ . ﮔﯿ ﺮﻣآ رد كزار هﺎ ﯾ

ﺎﮑ

ﻣ هداد ﺖﺸﮐ ﺎﭘورا و ﯽ

ا رد و دﻮﺷ ﯾ

ﻧ ناﺮ ﯿ ﻟﺎﻤﺷ ﻖﻃﺎﻨﻣ رد ﺰ ﯽ

ﻣ ﺪﺷر ﯽ ﺪﻨﮐ . اﯾ ﮔ ﻦ ﯿ وﺎﺣ هﺎ ي ﻦﻏور ﺎﻫ ي ﮐﺮﺗ ،راﺮﻓ ﯿ ﻠﭘ تﺎﺒ ﯽ -

ﻟﻮﻨﻓ ﯽ ﺋﻮﻧوﻼﻓ و ﯿ

ﮐﺮﺗ ،ﺎﻫﺪ ﯿ زر تﺎﺒ ﯾﻨ ﯽ ﺌﺗوﺮﭘ ، ﯿ ﻦ ﻟ ،ﺎﻫ ﯿﭙﯿ ،ﺎﻫﺪ

ﺳا ﯿ ﺎﻫﺪ ي ﺳا ،ﺎﻫﺪﻨﻗ ،بﺮﭼ ﯿ

ﺎﻫﺪ ي ﻣآ ﯿ ﻣ حﻼﻣا و ﻪﻨ ﯽ

ﺪﺷﺎﺑ . ﮔﯿ هﺎ

زار ﻔﻠﺘﺨﻣ تاﺮﺛا ك ﯽ

ﻣ ﺪﺿ ﻪﻠﻤﺟ زا ﯿ

ﺑوﺮﮑ ﯽ ﺘﻧآ ، ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ، ﭼرﺎﻗ ﺪﺿ

ﯽ ﻧﺎﻃﺮﺳ ﺪﺿ و ﯽ

دراد . ﻨﭽﻤﻫ ﯿ ا ﻦ ﯾ ﮔ ﻦ ﯿ ﺶﻫﺎﮐ رد هﺎ

ﻟﻮﺴﻧا ﺖﻣوﺎﻘﻣ ﯿ

اﺰﻓا ،ﻦ ﯾ ﺷ ﻢﺠﺣ ﺶ ﯿ

باﻮﺧ و هﺪﻌﻣ هﺮ روآ

ي ﺶﻘﻧ

اراد و ﻪﺘﺷاد ي

ﻣ بﺎﻬﺘﻟاﺪﺿ و درد ﺪﺿ تاﺮﺛا ﯽ

ﺪﺷﺎﺑ . ﮔﯿ كزار هﺎ

ﺎﻨﺻ رد ﯾ وراد ﻊ ﯾ ﯽ ﺷﻮﻧ و ﯿ ﻧﺪ ﯽ ﺷﻮﻧ ﺪﻨﻧﺎﻣ ﯿ ﻧﺪ ﯽ ﺠﺑآ و ناﻮﻨﻋ ﻪﺑ ﻮ

ﺒﺜﺗ ﯿ ﻣ هدﺎﻔﺘﺳا هﺪﻨﻫد ﻢﻌﻃ و ﻒﮐ هﺪﻨﻨﮐ ﺖ ﯽ

دﻮﺷ ] 7 , 8 [ . رد

نارﺎﮑﻤﻫ و نﺎﺒﺘﺳا ﻮﺴﻧﻮﻟآ ﺶﻫوﮋﭘ ]

9 [ دﺮﮔ ﺺﺨﺸﻣ ﯾ

ﻪﮐ ﺪ

ﮔ ﻪﻧاد هرﺎﺼﻋ ﯿ

اراد كزار هﺎ ي

ﺘﻧآ ﺮﺛا ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣ ﺪﺿ و ﯿ ﺑوﺮﮑ ﯽ

ﻣ ﯽ ﺪﺷﺎﺑ . ﻨﭽﻤﻫ ﯿ ﻣ ﺪﺿ ﺮﺛا ﻦ ﯿ

ﺑوﺮﮑ ﯽ ﺘﻧآ و ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ و گﺮﺑ

ﺖﺳا هﺪﺷ شراﺰﮔ كزار طوﺮﺨﻣ ]

10 [ . ﺗ ﺎﺑ ﺪﺿ صاﻮﺧ ﻪﺑ ﻪﺟﻮ

ﻣﯿ ﺑوﺮﮑ ﯽ ﺘﻧآ و ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﮔ ﯿ ا رد ،كزار هﺎ ﯾ

ﻣ ﺶﻫوﮋﭘ ﻦ ﯿ

ناﺰ

ﺋﻮﻧوﻼﻓ ،ﻞﮐ لﻮﻨﻓ ﯿ

ﺻﺎﺧ ،ﻞﮐ ﺪ ﯿ

ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﺎﻌﻓ و ﯿ ﺪﺿ ﺖ

ﻣﯿ ﺑوﺮﮑ ﯽ ا هرﺎﺼﻋ ﯾ ﮔ ﻦ ﯿ ور ﺮﺑ هﺎ ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﺪﻨﻧﺎﻣ نژﻮﺗﺎﭘ

ﺳرﺮﺑ ﯽ دﺮﮔ ﯾﺪ .

2 - شور و داﻮﻣ ﺎﻫ

2 - 1 - داﻮﻣ

ﺷ داﻮﻣ ﻪﻠﻤﺟ زا ﯿﻤ

ﯿﺎﯾ ﯽ د هدﺎﻔﺘﺳا درﻮﻣ ا ر

ﯾ ﻣ ﺶﻫوﮋﭘ ﻦ ﯽ

ﻪﺑ ناﻮﺗ

دار ﯾ دازآ لﺎﮑ ﻟﻮﻓ فﺮﻌﻣ ،DPPH

ﯿ ﻦ - ﺳ ﯿ دار ،ﻮﺘﻟﺎﮐﻮ ﯾ

دازآ لﺎﮑ

) ABTS ﺳ ﺖﮐﺮﺷ ﯿ ﺮﻣآ ،ﺎﻤﮕ ﯾ ﺎﮑ ( ﺤﻣ و ﯿ ﻂ ﺎﻫ ي ﺮﻟﻮﻣ ﺖﺸﮐ

ﻫﯿ ﻫ ﺮﻟﻮﻣ و رﺎﮔآ نﻮﺘﻨ ﯿ

ثاﺮﺑ نﻮﺘﻨ )

نﺎﻤﻟآ ،كﺮﻣ ﺖﮐﺮﺷ (

دﺮﺑ مﺎﻧ .

2 - 2 - ﻊﻤﺟ روآ ي ﮔ ﯿ ﻬﺗ و هﺎ ﯿ هرﺎﺼﻋ ﻪ

ﻊﻤﺟ زا ﺲﭘ روآ

ي ﮔ ﯿ اﺮﺷ رد ،كزار هﺎ ﯾ

ﮏﺸﺧ ﺐﺳﺎﻨﻣ ﻂ و هﺪﺷ

اﺮﺑ ي هرﺎﺼﻋ ﮔﯿ ﺮ ي دﺮﮔ ردﻮﭘ ﯾﺪ . ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ

زا هدﺎﻔﺘﺳا ﺎﺑ

ﺧ شور ﯿ ﻬﺗ نﺪﻧﺎﺴ ﯿ ﺪﺷ ﻪ . ﻬﺗ ﺖﻬﺟ ﯿ ،هرﺎﺼﻋ ﻪ 100 ﮔ زا مﺮﮔ ﯿ هﺎ

ﺎﺑ هﺪﺷ ردﻮﭘ 500

ﻣ ﯿﻠ ﯽ ﻟﯿ لﻮﻧﺎﺗا ﺮﺘ 96 تﺪﻣ ﻪﺑ و طﻮﻠﺨﻣ ﺪﺻرد

24 ﺎﻣد رد ﺖﻋﺎﺳ ي

ﺎﻣزآ ﯾ اﺮﺷ ﺖﺤﺗ هﺎﮕﺸ ﯾ

ﻧﺰﻤﻫ ﻂ ﯽ راﺮﻗ

ﺖﻓﺮﮔ . تﺪﻣ ﻪﺑ طﻮﻠﺨﻣ ﺲﭙﺳ 20

ﻗد ﯿ ﻪﻠﻌﺷ ﺎﺑ ﻪﻘ تراﺮﺣ ﻢﮐ

ﺪﺷ هداد . ﺖﻋﺮﺳ ﺎﺑ ﻞﺻﺎﺣ ﺖﻧﺎﺗﺎﻧﺮﭘﻮﺳ 3000

ﻗد رد رود ﯿ

ﻪﻘ

ﺮﺘﻧﺎﺳ ﯾﻔ ﯿ ﺪﺷ ژﻮ . دﺎﻘﻣ ﻪﺑ هﺪﻣآ ﺖﺳد ﻪﺑ ﺖﻧﺎﺗﺎﻧﺮﭘﻮﺳ ﻢﺠﺣ ﯾ

ﺮ

ﻠﺻا ﯽ ﺳر ﯿ ﺮﻄﻗ ﺎﺑ ﻦﻤﺗاو ﺬﻏﺎﮐ ﺎﺑ و هﺪ 45

/0 ﻣ ﯿ ﻓ ﺮﺘﻣوﺮﮑ ﯿ ﺪﺷ ﺮﺘﻠ .

نﻮﻣزآ مﺎﺠﻧا ﺖﻬﺟ طﻮﻠﺨﻣ ﺎﻫ

ي ،ﻪﺘﺴﺑ رد فوﺮﻇ رد ﻒﻠﺘﺨﻣ

رد و رﻮﻧ زا رود ﯾ

راﺪﻬﮕﻧ لﺎﭽﺨ ي

ﺪﻧﺪﺷ ] 11 [.

2 - 3 - هزاﺪﻧا ﮔﯿ ﺮ ي ﻞﮐ لﻮﻨﻓ

ﻟﻮﻓ شور زا هدﺎﻔﺘﺳا ﺎﺑ ﻞﮐ لﻮﻨﻓ ﯿ

ﻦ - ﺳ ﯿ هزاﺪﻧا ﻮﭽﻟﺎﮐﻮ ﮔﯿ

ﺮ ي

دﺮﮔ ﯾ ﺪ . 5/

0 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺎﺑ هرﺎﺼﻋ زا ﺮﺘ 5/

0 ﻣ ﯿﻠ ﯽ ﻟﯿ ﻟﻮﻓ فﺮﻌﻣ ﺮﺘ ﯿ

ﻦ -

ﺳ ﯿ و ﻮﭽﻟﺎﮐﻮ 1

ﻣ ﯿﻠ ﯽ ﻟﯿ ﺪﺳ ﺮﺘ ﯾ تﺎﻨﺑﺮﮐ ﻢ )

1 رﻻﻮﻣ ( طﻮﻠﺨﻣ

(3)

دﺮﮔ ﯾﺪ . زا ﺲﭘ 1 لﻮﻃ رد ﻪﻧﻮﻤﻧ بﺬﺟ ﺖﻋﺎﺳ جﻮﻣ

760 ﺮﺘﻣﻮﻧﺎﻧ

هزاﺪﻧا ﮔﯿ ﺮ ي ﺪﺷ . ﻣﯿ ﻣ ﺐﺴﺣ ﺮﺑ ﻞﮐ لﻮﻨﻓ ناﺰ ﯿﻠ

ﯽ ﻟﺎﮔ مﺮﮔ ﯿ ﺳا ﮏ ﯿ ﺪ

هرﺎﺼﻋ مﺮﮔ ﺮﻫ رد دﻮﺟﻮﻣ )

mg GAE/g (

دﺮﮔ شراﺰﮔ ﯾ

ﺪ

] 12 [ .

2 - 4 - ﻣ ﯿ ﺋﻮﻧوﻼﻓ ناﺰ ﯿ

ﻞﮐ ﺪ

25 /1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺎﺑ ﺮﻄﻘﻣ بآ ﺮﺘ 1

ﻣ ﯿﻠ ﯽ ﻟﯿ ﺪﺷ طﻮﻠﺨﻣ هرﺎﺼﻋ ﺮﺘ .

ﺲﭙﺳ 75 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﻧ لﻮﻠﺤﻣ ﺮﺘ ﯿ

ﺮﺘ ﯾ ﺪﺳ ﺖ ﯾ دﺮﮔ ﻪﻓﺎﺿا نآ ﻪﺑ ﻢ ﯾ

ﺪ .

ﺖﺷﺬﮔ زا ﺲﭘ 6

ﻗد ﯿ ﻮﻠﺤﻣ ﻪﻘ ﻣﻮﻟآ ل ﯿﻨﯿ ﺮﺗ مﻮ ي اﺮﻠﮐ ﯾ ﻪﻓﺎﺿا ﺪ

ﺪﺷ . زا ﺲﭘ 6 ﻗد ﯿ ﻪﻘ 1 ﻣ ﯿﻠ ﯽ ﻟﯿ دﺮﮔ ﻪﻓﺎﺿا لﻮﻠﺤﻣ ﻪﺑ دﻮﺳ ﺮﺘ ﯾ

و ﺪ

رد ﻪﻧﻮﻤﻧ بﺬﺟ 510

ﺪﺷ ﺖﺒﺛ ﺮﺘﻣﻮﻧﺎﻧ .

ﺋﻮﻧووﻼﻓ راﺪﻘﻣ ﯿ

ﻞﮐ ﺪ

ﻣ ﺐﺴﺣﺮﺑ ﯿﻠ ﯽ ﺘﺳﺮﺋﻮﮐ مﺮﮔ ﯿ

هرﺎﺼﻋ مﺮﮔ ﺮﻫ رد ﻦ )

mg QE/g (

دﺮﮔ شراﺰﮔ ﯾ

ﺪ ] 13 [.

2 - 5 - ﻌﺗ ﯿﯿ ﻟﺎﻌﻓ ﻦ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ هرﺎﺼﻋ

شور ﺎﻫ ي ﻬﻣ دار رﺎ ﯾ دازآ لﺎﮑ و DPPH

دار رﺎﻬﻣ ﯾ دازآ لﺎﮑ

ABTS ﻌﺗ ﺖﻬﺟ ﯿﯿ ﺻﺎﺧ ﻦ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻬﺗ هرﺎﺼﻋ ﯿ هﺪﺷ ﻪ

ﺪﺷ هدﺎﻔﺘﺳا .

2 - 5 - 1 - هزاﺪﻧا ﮔﯿ ﺮ ي دار رﺎﻬﻣ ﯾ دازآ لﺎﮑ DPPH

ﮔ هرﺎﺼﻋ كﻮﺘﺳا لﻮﻠﺤﻣ ﯿ

ﻫﺎ ﯽ ﺳر ﺖﻬﺟ لﻮﻧﺎﺗا رد ﯿ

ﻪﺑ نﺪ

ﺖﻈﻠﻏ 1 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﻬﺗ ﺮﺘ ﯿ دﺮﮔ ﻪ ﯾﺪ . 1 ﻣ ﯿﻠ ﯽ ﻟﯿ زا ﺮﺘ

ﺑ هرﺎﺼﻋ ﺎ 1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﻟﻮﻧﺎﺘﻣ لﻮﻠﺤﻣ ﺮﺘ ﯽ

) DPPH 2/

0 ﻣ ﯿﻠ ﯽ رﻻﻮﻣ (

ﺪﺷ طﻮﻠﺨﻣ .

تﺪﻣ زا ﺲﭘ 30

ﻗد ﯿ ﺎﻣد رد ﻪﻘ ي رﺎﺗ رد و قﺎﺗا ﯾﮑ

ﯽ ،

ﻪﻧﻮﻤﻧ بﺬﺟ لﻮﻃ رد ﺎﻫ

جﻮﻣ 517 هزاﺪﻧا ﺮﺘﻣﻮﻧﺎﻧ ﮔﯿ

ﺮ ي ﺪﺷ . زا

لﻮﻠﺤﻣ ﺎﻫ ي ﻪﻧﻮﻤﻧ ناﻮﻨﻋ ﻪﺑ هرﺎﺼﻋ نوﺪﺑ هﺪﺷ ﻪﺘﻓﺮﮔ رﺎﮐ ﻪﺑ

ﺪﺷ لﺮﺘﻨﮐ . ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺪﺻرد ﯽ

دار ﯾ لﺎﮑ ﺘﺳا ﺎﺑDPPH زا هدﺎﻔ

ز ﻪﻟدﺎﻌﻣ ﯾ دﺮﮔ ﻪﺒﺳﺎﺤﻣ ﺮ ﯾ

ﺪ ] 2 [:

= ﻟﺎﻌﻓ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖ ﯽ

) DPPH ﺪﺻرد (

100

× )

لﺮﺘﻨﮐ

/ A

ﻪﻧﻮﻤﻧ

-A

لﺮﺘﻨﮐ

(A

2 - 5 - 2 - دار رﺎﻬﻣ ﯾ دازآ لﺎﮑ ABTS

دار ﯾ لﺎﮑ ﺎﻫ ي دازآ ABTS ) 7 ﻣ ﯿﻠ ﯽ رﻻﻮﻣ ( ﺳﺎﺘﭘ ندوﺰﻓا ﺎﺑ ﯿ

ﻢ

ﺎﺑ تﺎﻔﻟﻮﺳﺮﭘ 45

/2 ﻣ ﯿﻠ ﯽ ﻗر رﻻﻮﻣ ﯿ ﺎﻣد رد و ﻖ ي

رﺎﺗ رد قﺎﺗا ﯾﮑ

ﯽ

راﺪﻬﮕﻧ ي دﺮﮔ ﯾﺪ . ﻣ ﺗﺎﮐ لﻮﻠﺤ ﯿ ﻧﻮ ﯽ دار ﯾ ﻟﺎﮑ ﯽ ﺳر ﺎﺗ ABTS ﯿ نﺪ

بﺬﺟ ﻪﺑ 7/

0 لﻮﻃ رد جﻮﻣ 734 ﻗر ﺮﺘﻣﻮﻧﺎﻧ ﯿ ﺪﺷ ﻖ . ﻪﻠﺣﺮﻣ رد

ﺪﻌﺑ 100 ﻣ ﯿ وﺮﮑ ﻟﯿ هرﺎﺼﻋ زا ﺮﺘ ﯾ

لﻮﻧﺎﺗا ﺎ ) لﺮﺘﻨﮐ ناﻮﻨﻋ ﻪﺑ (

ﻪﺑ 9/

3

ﻣﯿ ﻠ ﯽ ﻟﯿ ﺗﺎﮐ لﻮﻠﺤﻣ ﺮﺘ ﯿ

ﻧﻮ ﯽ دار ﯾ لﺎﮑ ﺪﺷ ﻪﻓﺎﺿا ABTS

. زا ﺲﭘ 6

ﻗد ﯿ هزاﺪﻧا ﻪﻧﻮﻤﻧ بﺬﺟ ﻪﻘ ﮔﯿ

ﺮ ي ﻟﺎﻌﻓ و ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖ ﯽ

ﻖﺒﻃ

ز لﻮﻣﺮﻓ ﯾ دﺮﮔ ﻪﺒﺳﺎﺤﻣ ﺮ ﯾ

ﺪ ] 3 [:

100

× )

لﺮﺘﻨﮐ

/A

ﻪﻧﻮﻤﻧ

-A

لﺮﺘﻨﮐ

= (A ﻟﺎﻌﻓ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖ ﯽ

) ﺪﺻرد (

2 - 6 - ﻌﺗ ﯿﯿ ﻟﺎﻌﻓ ﻦ ﯿ ﻣﺪﺿ ﺖ ﯿ

ﺑوﺮﮑ ﯽ هرﺎﺼﻋ

ﺻﺎﺧ ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﺮﺘﮐﺎﺑ ﺮﺑاﺮﺑ رد هرﺎﺼﻋ ي

ﺎﻫ ي مﺮﮔ نژﻮﺗﺎﭘ

ﺖﺒﺜﻣ ) Staphylococcus aureus و

Listeria

monocytogenes (

ﻔﻨﻣ مﺮﮔ و ﯽ ) Escherichia coli و

Enterobacter aerogenes (

شور زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ

ي

دﯾ د ﮏﺴ ﯾﻔ ﯿ هزاﺪﻧا ،رﺎﮔآ ﮏﻫﺎﭼ ،رﺎﮔآ نژﻮ ﮔﯿ

ﺮ ي ﺖﻈﻠﻏ ﻞﻗاﺪﺣ

ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﮔﺪﻨﺸﮐ و ﯽ زرا ﯾﺑﺎ ﯽ دﺮﮔ ﯾﺪ .

2 - 6 - 1 - د ﯾ د ﮏﺴ ﯾﻔ ﯿ رﺎﮔآ نژﻮ

د اﺪﺘﺑا ﯾ ﮏﺴ ﺎﻫ ي ﺮﺘﺳا ﮏﻧﻼﺑ ﯾ

تﺪﻣ ﻪﺑ ﻞ 20

ﻗد ﯿ ﺮﻫ رد ﻪﻘ ﯾ زا ﮏ

ﺖﻈﻠﻏ ﺎﻫ ي ﻬﺗ ﯿ هرﺎﺼﻋ زا هﺪﺷ ﻪ ﺎﻫ

ي ﻟﻮﻧﺎﺗا ﯽ ﮔ ﯿ كزار هﺎ ) 100 ،

200 ، 300 و 400 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﻪﻃﻮﻏ ﺪﺷ رو . دﯾ ﮏﺴ -

ﺮﻫ ﺖﺸﮐ زا ﺲﭘ ﺎﻫ ﯾ

ﺮﺘﮐﺎﺑ زا ﮏ ي

ﺎﻫ ي ور ﻪﻌﻟﺎﻄﻣ درﻮﻣ ي

ﺤﻣ ﯿ ﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂ ﯿ

ﺒﺜﺗ رﺎﮔآ نﻮﺘﻨ ﯿ

دﺮﮔ ﺖ ﯾ ﺪ . زا ﺲﭘ 24 ﺖﻋﺎﺳ

ﻪﻧﺎﺨﻣﺮﮔ راﺬﮔ ي ﺎﻣد رد ي 37 ﺘﻧﺎﺳ ﻪﺟرد ﯽ

ﻪﻟﺎﻫ ﺮﻄﻗ ،داﺮﮔ ﺎﻫ

ي

د فاﺮﻃا ﺪﺷر مﺪﻋ ﯾ

ﮏﺴ ﺐﺴﺣ ﺮﺑ ﺎﻫ ﻣﯿ

ﻠ ﯽ هزاﺪﻧا ﺮﺘﻣ ﮔﯿ ﺮ ي

ﺪﺷ ] 14 [.

2 - 6 - 2 - رﺎﮔآ ﮏﻫﺎﭼ

ا رد ﯾ ﺴﻧﺎﭙﺳﻮﺳ ﺖﺸﮐ ﺲﭘ شور ﻦ ﯿ

نﻮ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ) لدﺎﻌﻣ

دراﺪﻧﺎﺘﺳا 5/

0 ﺪﻨﻟرﺎﻓ ﮏﻣ (

ﺤﻣ ﺢﻄﺳ رد ﯿ

ﻫ ﺮﻟﻮﻣ ﻂ ﯿ ،رﺎﮔآ نﻮﺘﻨ

60 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﻫ زا ﺮﺘ ﯾ ﺖﻈﻠﻏ زا ﮏ ﺎﻫ

ي ﻬﺗ ﯿ هرﺎﺼﻋ زا هﺪﺷ ﻪ

) 100 ، 200 ، 300 و 400 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﮏﻫﺎﭼ ﻪﺑ ﻫﺎ ﯾ ﯽ

ﺮﻄﻗ ﺎﺑ 6 ﻣ ﯿﻠ ﯽ ا ﺮﺘﻣ ﯾ ﺤﻣ رد هﺪﺷ دﺎﺠ ﯿ

ر ﺖﺸﮐ ﻂ ﯾ

ﺪﺷ ﻪﺘﺨ .

ﻠﭘﯿ ﺖ تﺪﻣ ﻪﺑ ﺎﻫ 24

ﺎﻣد رد ﺖﻋﺎﺳ ي

37 ﺘﻧﺎﺳ ﻪﺟرد ﯽ

داﺮﮔ

ﻪﻧﺎﺨﻣﺮﮔ راﺬﮔ ي ﺪﻧﺪﺷ . ﻪﻟﺎﻫ ﺮﻄﻗ ﺎﻫ ي ﺮﻫ فاﺮﻃا ﺪﺷر مﺪﻋ ﯾ

ﮏ

ﮏﻫﺎﭼ زا ﻣ ﺐﺴﺣ ﺮﺑ ﺎﻫ ﯿﻠ

ﯽ هزاﺪﻧا ﺮﺘﻣ ﮔﯿ ﺮ ي ﺪﺷ ] 15 [ .

2 - 6 - 3 - هزاﺪﻧا ﮔﯿ ﺮ ي ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ

ﺖــﻬﺟ هزاﺪــﻧا ﮔ ــﯿ ﺮ ي ﮔﺪــﻨﻨﮐرﺎﻬﻣ ﺖــﻈﻠﻏ ﻞﻗاﺪــﺣ ﯽ

شور زا

ﺖﻗر زﺎﺳ ي ﺎﻣ رد ﯾ ﻣ رد ﻊ ﯿ ﻠﭘوﺮﮑ ﯿ ﺖ ﺎﻫ ي 96 ﻪﻧﺎﺧ ا ي ﺪﺷ هدﺎﻔﺘﺳا .

ﺪﺑ ﯾ ﺗﺮﺗ ﻦ ﯿ ﻪﮐ ﺐ 100 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺖـﻗر زا ﺮﺘ ﺎـﻫ

ي ﻟاﻮـﺘﻣ ﯽ هرﺎﺼـﻋ

ﻟﻮﻧﺎـــﺘﻣ ﯽ كزار ) 2 ، 4 ، 8 ، 16 ، 32 ، 64 ، 128 ، 256 و 512 ( رد ،

ﮏﻫﺎﭼ ﺎﻫ ي ﻠﭘ ﯿ ﺖ 96 ﻪـﻧﺎﺧ ا ي ر ﯾ و ﺪـﺷ ﻪـﺘﺨ 20

ﻣ ﯿ ﻟوﺮﮑ ﯿ زا ﺮـﺘ

ﺴﻧﺎﭙﺳﻮﺳ ﯿ ﻣ نﻮ ﯿ ﺑوﺮﮑ ﯽ دﺮﮔ ﻪﻓﺎﺿا نآ ﻪﺑ ﯾ

ﺪ . ﻪـﮐ ﺮﺧآ ﮏﻫﺎﭼ ود

ﯾﮑ ﯽ وﺎﺣ ي ﺴﻧﺎﭙﺳﻮﺳ ﯿ ﻣ نﻮ ﯿ ﺑوﺮﮑ ﯽ ﺤﻣ و ﯿ ناﻮﻨﻋ ﻪﺑ دﻮﺑ ﺖﺸﮐ ﻂ

د ﮏﻫﺎﭼ و ﺖﺒﺜﻣ لﺮﺘﻨﮐ ﯾ

وﺎﺣ ﻪﮐ ﺮﮕ ي

ﺤﻣ ﯿ هرﺎﺼـﻋ و ﺖﺸـﮐ ﻂ

ﻟﻮﻧﺎﺗا ﯽ ﻔﻨﻣ لﺮﺘﻨﮐ ناﻮﻨﻋ ﻪﺑ دﻮﺑ ﯽ

ﺪـﺷ ﻪـﺘﻓﺮﮔ ﺮـﻈﻧ رد .

زا ﺲـﭘ

ﺖــﺷﺬﮔ 24 ﺎــﻣد رد ﺖﻋﺎــﺳ ي

37 ﺘﻧﺎــﺳ ﻪــﺟرد ﯽ

ــﮔ ،داﺮ 10

ﻣﯿ ﻟوﺮﮑ ﯿ ﺮﺗ فﺮﻌﻣ زا ﺮﺘ ي

ﻞﻨﻓ ﻟوزاﺮﺘﺗ ﯿ مﻮ ) 5 ﺪﺻرد ( ﮏﻫﺎﭼ ﻪﺑ ﺎـﻫ

(4)

ﺪﺷ ﻪﻓﺎﺿا . تﺪﻣ ﻪﺑ ًادﺪﺠﻣ 5/

0 ﻪﻧﺎﺨﻣﺮﮔ ﺖﻋﺎﺳ راﺬﮔ

ي راﺮـﮑﺗ

دﺮﮔ ﯾﺪ . ﻟوا ﯿ ﻪﻧﺎﺧ ﻦ ا ي ﻫ نآ رد ﻪﮐ ﯿ

ﻐﺗ و ﺪﺷر ﻪﻧﻮﮕﭽ ﯿ

ـﯿ ـﮕﻧر ﺮ ﯽ

ﮔﺪـﻨﻨﮐرﺎﻬﻣ ﺖـﻈﻠﻏ ﻞﻗاﺪـﺣ ناﻮﻨﻋ ﻪﺑ ،ﺪﺸﻧ هﺪﻫﺎﺸﻣ ﯽ

شراﺰـﮔ

دﺮﮔ ﯾ ﺪ ] 16 [ .

2 - 6 - 4 - هزاﺪﻧا ﮔﯿ ﺮ ي ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ

100 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﻫ زا ﺮﺘ ﯾ ﮏﻫﺎﭼ زا ﮏ ﺎﻫ

ي ﻐﺗ نوﺪﺑ ﯿ ـﯿ ﺮـﺑ ﮓـﻧر ﺮ

ور ي ﺤﻣ ﯿ ﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂ ﯿ

ﺪـﺷ هداد ﺖﺸـﮐ رﺎﮔآ نﻮﺘﻨ .

زا ﺲـﭘ

ﻪﻧﺎﺨﻣﺮﮔ راﺬﮔ ي اﺮﺷ ﺪﻨﻧﺎﻣ ﯾ ﺮـﺘﻤﮐ ،قﻮﻓ ﻂ ﯾ

ـﺘﻈﻠﻏ ﻦ ﯽ نآ رد ﻪـﮐ

ﺮﺘﮐﺎﺑ ﺪﺷر ي ﮔﺪﻨﺸـﮐ ﺖـﻈﻠﻏ ﻞﻗاﺪـﺣ ناﻮﻨﻋ ﻪﺑ ﺪﺸﻧ هﺪﻫﺎﺸﻣ ﯽ

ﺪﺷ شراﺰﮔ ]

17 [ .

2 - 7 - ﻟﺎﻧآ ﯿ رﺎﻣآ ﺰ ي

نﻮﻣزآ ﺎﻫ ي ا ﯾ ﺪﻧﺪـﺷ مﺎـﺠﻧا راﺮـﮑﺗ ﻪﺳ رد ﺶﻫوﮋﭘ ﻦ .

ﺰﺠﺗ ـﯾ و ﻪ

ﻠﺤﺗ ﯿ ﺎﺘﻧ ﻞ ﯾ مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﺞ راﺰﻓا

ﻪﺨﺴﻧ SPSS 18 ﺪـﺷ مﺎﺠﻧا .

ﺨﺸﺗ ﺖﻬﺟ ﯿ

ﻨﻌﻣ ﺺ ﯽ ﺑ فﻼﺘﺧا ندﻮﺑ راد ﯿ

ﻣ ﻦ ﯿ ﮕﻧﺎ ﯿ ﻦ ﻟﺎﻧآ زا ،ﺎﻫ ﯿ ﺰ

راو ﯾ ﺲﻧﺎ ﯾ ﮏ ﻤﻃا ﺢﻄﺳ رد ﻦﮑﻧاد نﻮﻣزآ و ﻪﻓﺮﻃ ﯿ

نﺎﻨ 95 ﺪـﺻرد

) p<0.05 ( دﺮﮔ هدﺎﻔﺘﺳا ﯾﺪ

.

3 - ﺎﺘﻧ ﯾ ﺞ ﺚﺤﺑ و

3 - 1 - ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ

ﺑوﺮﮑ ﯽ

ﺎﺘﻧ ﯾ ﻟﺎﻌﻓ ﺞ ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﺖﻈﻠﻏ ﺎﻫ ي ﻟﻮﻧﺎـﺗا هرﺎﺼـﻋ ﻒﻠﺘﺨﻣ ﯽ

ﺮﺘﮐﺎﺑ ﺮﺑ كزار ي

ﺎﻫ ي د شور سﺎﺳا ﺮﺑ ،نژﻮﺗﺎﭘ ـﯾ

د ﮏﺴ ﯾﻔ ـﯿ نژﻮ

ﻞﮑﺷ رد ،رﺎﮔآ 1

ﺖﺳا هﺪﺷ هدروآ .

ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ اﺰﻓا ،ﺞ ﯾ ﺖﻈﻠﻏ ﺶ

اﺰﻓا ﺐﺒﺳ هرﺎﺼﻋ ﯾ

ﻨﻌﻣ ﺶ ﯽ ﮔﺪﻧرادزﺎﺑ ﻪﻟﺎﻫ ﺮﻄﻗ راد ﯽ

دﺮﮔ ـﯾ ﺪ . رد

ﺎﺑ ﺎﺑ طﺎﺒﺗرا ﺮﺘﮐ ي E. aerogenes ﮔﺪـﻧرادزﺎﺑ ﻪـﻟﺎﻫ ﺮـﻄﻗ ،

ﯽ زا

3/

6 ﻣ ﯿﻠ ﯽ ﺖﻈﻠﻏ رد ﺮﺘﻣ 100

ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﻪـﺑ هرﺎﺼـﻋ ﺮـﺘ

5/

12 ﻣ ﯿﻠ ﯽ ﺖﻈﻠﻏ رد ﺮﺘﻣ 400

ﻣ ﯿﻠ ﯽ ﻣ رد مﺮـﮔ ﯿ ـﻠ ﯽ ﻟﯿ اﺰـﻓا ﺮـﺘ ﯾ ﺶ

ﯾ ﺖﻓﺎ . ﮔﺪﻧرادزﺎﺑ ﻪﻟﺎﻫ ﺮﻄﻗ ﯽ

اﺮﺑ ي ﺮﺘﮐﺎـﺑ ي E. coli ﺖـﻈﻠﻏ رد

100 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺎﺑ ﺮﺑاﺮﺑ هرﺎﺼﻋ ﺮﺘ 8/

6 ﻣ ﯿﻠ ﯽ ﺎـﻣا ،دﻮﺑ ﺮﺘﻣ

ﺖﻈﻠﻏ 400 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ا ﺐﺒﺳ هرﺎﺼﻋ ﺮﺘ ﯾ

مﺪﻋ ﻪﻟﺎﻫ دﺎﺠ

ﺮﻄﻗ ﺎﺑ ﺪﺷر 14

ﻣ ﯿﻠ ﯽ ﻨﻠﮐ فاﺮﻃا رد ﺮﺘﻣ ﯽ

ﺎﻫ ي ﺮﺘﮐﺎـﺑ ي E. coli

دﺮﮔ ﯾﺪ . ﺎﺘﻧ ﯾ ﻬﺑﺎﺸﻣ ﺞ ﯽ ﺮﺘﮐﺎﺑ درﻮﻣ رد ي

ﺎﻫ ي ﺪـﺷ هﺪﻫﺎﺸـﻣ .

ﺮـﻄﻗ

رﻮﻀﺣ رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ 100

ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﻪـﺑ هرﺎﺼﻋ ﺮﺘ

ﺗﺮﺗ ﯿ ﺐ 8/

8 و 3/

7 ﻣ ﯿﻠ ﯽ اﺮﺑ ﺮﺘﻣ ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي S. aureus و

L.

monocytogenes اﺰــﻓا و دﻮــﺑ

ﯾ ﻪــﺑ هرﺎﺼــﻋ ﺖــﻈﻠﻏ ﺶ 400

ﻣﯿ ﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ اﺰﻓا ﺐﺒﺳ ﺮﺘ ﯾ

ﻨﻌﻣ ﺶ ﯽ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ راد

) ﺗﺮﺗ ﻪﺑ ﯿ ﺐ 9/

16 و 5/

15 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ﺪﺷ . ﻠﮐ رﻮﻄﺑ ﯽ گرﺰﺑ ، ﺮـﺗ ﯾ ﻦ ﮏﭼﻮﮐ و

ﺮﺗ ﯾ ﺗﺮﺗ ﻪﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦ ﯿ

ﺮﺘﮐﺎﺑ رد ﺐ ي

ﺎﻫ ي S.

aureus و E. aerogenes دﺮﮔ هﺪﻫﺎﺸﻣ

ـﯾ ﺮﺘﮐﺎـﺑ و ﺪ ي ﺎـﻫ ي

ﺖﺒﺜﻣ مﺮﮔ ) S. aureus و

L. monocytogenes (

ﻪـﺑ ﺖﺒﺴﻧ

ﻔﻨﻣ مﺮﮔ عاﻮﻧا ﯽ

) E. coli و E. aerogenes (

هرﺎﺼﻋ ﺮﺑاﺮﺑ رد

ﻟﻮﻧﺎﺗا ﯽ سﺎﺴﺣ كزار ﺪﻧدﻮﺑ ﺮﺗ

.

Fig 1 Mean inhibition zone diameter (mm) of Humulus lupulus extract on some microorganism

pathogen (disk diffusion agar).

ﻞﮑﺷ 2 ﺎﺘﻧ ، ﯾ ﻣﺪﺿ ﺮﺛا ﺞ ﯿ

ﺑوﺮﮑ ﯽ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ

ﺮﺑاﺮﺑ رد كزار

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي نﺎﺸﻧ ار رﺎﮔآ ﮏﻫﺎﭼ رﺎﺸﺘﻧا شور سﺎﺳا ﺮﺑ نژﻮﺗﺎﭘ

ﻣ ﯽ ﺪﻫد . ﻣﺪﺿ ﺮﺛا ﺪﻧور ﯿ

ﺑوﺮﮑ ﯽ ﻏ ﺖﻈﻠ ﺎﻫ ي هرﺎﺼﻋ ﻒﻠﺘﺨﻣ

د شور ﻪﺑﺎﺸﻣ ﯾ

د ﮏﺴ ﯾﻔ ﯿ ﺎﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ و دﻮﺑ رﺎﮔآ نژﻮ

اﺰﻓا ﯾ ﻨﻌﻣ رﻮﻄﺑ هرﺎﺼﻋ ﺖﻈﻠﻏ ﺶ ﯽ

راد ي اﺰﻓا ﯾ ﺶ ﯾ ﺖﻓﺎ .

Fig 2 Mean inhibition zone diameter (mm) of Humulus lupulus extract on some microorganism

pathogen (well diffusion agar).

ا ﺮﺑ هوﻼﻋ ﯾ

ﺖﻈﻠﻏ رد ،ﻦ 400

ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ

ﯽ ﻟﯿ ،ﺮﺘ

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي S. aureus و

E. aerogenes ﺗﺮﺗ ﻪﺑ

ﯿ ﺮﺗﻻﺎﺑ ﺐ ﯾ ﻦ

(5)

) 6/

17 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ﺮﺘﻤﮐ و ﯾ ﻦ ) 9/

13 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ

ﻣﺪﺿ ﺮﺛا و ﺪﻧراد صﺎﺼﺘﺧا دﻮﺧ ﻪﺑ ار ﯿ

ﺑوﺮﮑ ﯽ ﺮﺑاﺮﺑ رد هرﺎﺼﻋ

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﮔ ﺑ ﺖﺒﺜﻣ مﺮ ﯿ

ﺮﺘﮐﺎﺑ زا ﺮﺘﺸ ي

ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ دﻮﺑ

)ﻣ ﯿ ﮕﻧﺎ ﯿ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦ 8/

16 ﻣ ﯿﻠ ﯽ ﺮﺑاﺮﺑ رد ﺮﺘﻣ 5/

14

ﻣﯿ ﻠ ﯽ ﺖﻈﻠﻏ رد ﺮﺘﻣ 400

ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ .(

ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ ﺞ

ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ

) لوﺪﺟ 1 ( ﺮﺘﮐﺎﺑ ﺪﺷر ، ي

ﺎﻫ ي مﺮﮔ

ﺖﺒﺜﻣ S. aureus و

L. monocytogenes رﻮﻀﺣ رد

ﺖﻈﻠﻏ ﺎﻫ ي ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﺮﺘﻤﮐ ﯽ

دﺮﮔ ﻒﻗﻮﺘﻣ كزار ﯾ

ﺮﺘﻤﮐ و ﺪ ﯾ ﻦ )

64 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﺑ و ﯿ ﺮﺘﺸ ﯾ ﻦ ) 256 ﻣ ﯿﻠ ﯽ رد مﺮﮔ

ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ( ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﯽ

ﺗﺮﺗ ﻪﺑ ﺪﺷر ﯿ

ﺮﺘﮐﺎﺑ رد ﺐ ي

ﺎﻫ ي S.

aureus و E. aerogenes ﺪﺷ هﺪﻫﺎﺸﻣ

. ﺎﺘﻧ ﯾ ﻞﻗاﺪﺣ ﺞ

ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﯽ

ﻧ ﯿ ﺮﺘﮐﺎﺑ ﻪﮐ داد نﺎﺸﻧ ﺰ ي

سﺎﺴﺣ ﺮﺗ ﯾ ﻦ

ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﺑ ﺖﺒﺴﻧ ﻢﺴ ﯽ

ﻣ كزار ﯽ و ﺪﺷﺎﺑ

ﺮﺘﻤﮐ ﺖﻈﻠﻏ ي

ﺑ زا ﺖﻬﺟ هرﺎﺼﻋ زا ﯿ

ا ندﺮﺑ ﻦ ﯾ ﺮﺘﮐﺎﺑ ﻦ ي ﻧ ﯿ زﺎ

ﺖﺳا ) لوﺪﺟ 1 .(

Table 1 The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Humuluslupulus extract on some pathogenic microorganisms.

ﺴﮐ ﻪﻌﻟﺎﻄﻣ رد ﺮ

ي ﻫﺎﺸﻧﺎﻣﺮﮐ ﯽ نارﺎﮑﻤﻫ و ]1

[ ﺮﺘﮐﺎﺑ ﺪﺿ ﺮﺛا ، ي

ﻠﮑﻟا هرﺎﺼﻋ ﯽ ﮔ ﻞﮔ ﯿ ور ﺮﺑ كزار هﺎ ي

ﻮﺳ رﺎﻬﭼ ﯾ ﻪ S. aureus ،

E. coli ، Pseudomonas aeruginosa و

Bacillus

subtilis ﺳرﺮﺑ ﯽ دﺮﮔ ﯾﺪ . ﻣﺎﻤﺗ رد كزار هرﺎﺼﻋ ﯽ

ﺖﻈﻠﻏ ﺎﻫ

ﻮﺑ ﯾ ﺖﻈﻠﻏ هﮋ ﺎﻫ ي 1000 و 500 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﻪﺑ ﺮﺘ رﻮﻃ

ﻨﻌﻣ ﯽ راد ي ر زا ﺮﺘﮐﺎﺑ ﺪﺷ ي ﺎﻫ ي ﮔﻮﻠﺟ ﺖﺒﺜﻣ مﺮﮔ ﯿﺮ

ي و دﻮﻤﻧ

ﺖﻈﻠﻏ ﺎﻫ ي 125 و 5/

62 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ اراد ﺮﺘ ي رﺎﻬﻣ ﺮﺛا ي

ﮔﺪﻨﺸﮐ و ﯽ ﺗﺮﺗ ﻪﺑ ﯿ ﺮﺘﮐﺎﺑ درﻮﻣ رد ﺐ ي

ﺎﻫ ي B. subtilis و

S.

aureus ﺪﻧدﻮﺑ ] 1 [ . ﺪﻤﺤﻣ ي ﻧﺎﺛ ﯽ نارﺎﮑﻤﻫ و ]

7 [ ﺳرﺮﺑ ﺎﺑ ﯽ ﺮﺛا

ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﺑآ هرﺎﺼﻋ ﯽ

ﺮﺑ كزار S. aureus

، L.

monocytogenes ،

E. aerogenes و

Salmonella

enterica د شور ﻖﺑﺎﻄﻣ ﻪﮐ ﺪﻧداد نﺎﺸﻧ

ﯾ د ﮏﺴ ﯾﻔ ﯿ ثاﺮﺑ و نژﻮ

ﻣﯿ دوﺮﮑ ﯾ ﺮﺘﮐﺎﺑ ،ﻦﺷﻮﻠ ي

ﺎﻫ ي ﺑآ هرﺎﺼﻋ ﻪﺑ ﺖﺒﺜﻣ مﺮﮔ ﯽ

كزار

ﺮﺘﮐﺎﺑ ﻪﺑ ﺖﺒﺴﻧ ي

ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ سﺎﺴﺣ ﺪﻧدﻮﺑ ﺮﺗ . ﺑﯿ ﺮﺘﺸ ﯾ ﺮﺛا ﻦ

ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﺪﺷر ) ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ 17

ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( شور رد

ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ د ﯾ د ﮏﺴ ﯾﻔ ﯿ ﺮﺑاﺮﺑ رد رﺎﮔآ نژﻮ S. aureus

ﺪﺷ هﺪﻫﺎﺸﻣ .

ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،ﻪﭼﺮﮔا ﯽ

هرﺎﺼﻋ ﺪﺷر

ﺑآ ﯽ ور ﺮﺑ كزار ي

ﺎﺑ ﺮﺑاﺮﺑ 625 /0 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺎﻣا ،دﻮﺑ ﺮﺘ

ﺖﻈﻠﻏ ﺎﻫ ي ﺳرﺮﺑ درﻮﻣ ﯽ

ﮔﺪﻨﺸﮐ ﺮﺛا هرﺎﺼﻋ ﯽ

) (MBC ﺮﺑ

ﺮﺘﮐﺎﺑ ي ﺪﻨﺘﺷاﺪﻧ ﺎﻫ ]

7 [ . ﻪﻌﻟﺎﻄﻣ رد ي نارﺎﮑﻤﻫ و نﺎﺒﺘﺳا ﻮﺴﻧﻮﻟآ

] 9 [ رﺎﺼﻋ ، ﻟﻮﻧﺎﺘﻣ ه ﯽ اراد كزار ي

ﺮﺘﮐﺎﺑ ﺪﺿ ﺮﺛا ﯾﺎﯾ

ﯽ ﺮﺑ

ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي ﺮﺑ S. aureus

، L. monocytogenes ،

Bacillus cereus ،

E. coli ،

Enterococcus

faecalis و Salmonella typhimurium ا و دﻮﺑ

ﯾ ﻪﺑ ﺮﺛا ﻦ

ﭽﺗﺎﮐ رﻮﻀﺣ ﯿ

بﻮﺳر ﻪﺑ ردﺎﻗ ﻪﮐ ﺪﺷ هداد ﺖﺒﺴﻧ هرﺎﺼﻋ رد ﻦ

ﺌﺗوﺮﭘ ﯿ ﻦ ﺎﻫ ي د ﯾ ﻟﻮﻠﺳ هراﻮ ﯽ رد و ﺘﻧ ﯿ ﮑﺸﺗ رد ﻞﺧاﺪﺗ ﻪﺠ ﯿ

د ﻞ ﯾ هراﻮ

ﻟﻮﻠﺳ ﯽ ﻣ ﯽ ﺪﻨﺷﺎﺑ ] 9 , 18 [ . ﺑ ﺖﻣوﺎﻘﻣ ﻪﮐ ﺖﺳا ﺮﮐذ ﻪﺑ مزﻻ ﯿ

ﺮﺘﺸ

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ هرﺎﺼﻋ ﺮﺑاﺮﺑ رد ﺖﺒﺜﻣ مﺮﮔ عاﻮﻧا ﻪﺑ ﺖﺒﺴﻧ

ﻟﻮﻧﺎﺗا ﯽ ﺷﺎﻧ ًﺎﺗﺪﻤﻋ كزار ﯽ

ﺎﻫءﺎﺸﻏ دﻮﺟو زا ي

ﺟرﺎﺧ ﯽ فاﺮﻃا

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ اﺰﻓا ﺐﺒﺳ ﻪﮐ ﺖﺳا ﯾ

نآ ﺖﻣوﺎﻘﻣ ﺶ ﺎﻫ

ﻣ ﯽ ددﺮﮔ . ﮔ ﺎﺸﻏ ﻪﮐ ﺖﺳا هﺪﺷ شراﺰ ي

ﺑ ﯿ ﻧوﺮ ﯽ ﺮﺘﮐﺎﺑ ي ﺎﻫ ي مﺮﮔ

ﻔﻨﻣ ﯽ ﭘ رﺎﺘﺧﺎﺳ زا ﯿ

ﭽ ﯿ ﻟ هﺪ ﯿ ﻠﭘﻮﭙ ﯽ رﺎﮐﺎﺳ ﯾ ﺪ ي ﮑﺸﺗ ﯿ ﻪﮐ ﺖﺳا هﺪﺷ ﻞ

ﮐﺮﺗ ذﻮﻔﻧ ﺖﻋﺮﺳ ﯿ

ﺮﮕﺑآ تﺎﺒ ﯾ ﺲﻧﺎﺳا و هرﺎﺼﻋ رد دﻮﺟﻮﻣ ﺰ ﺎﻫ

ي

ﮔﯿ ﻫﺎ ﯽ ﻣ ﺪﻨﮐ ار لﻮﻠﺳ ﻞﺧاد ﻪﺑ ﯽ

دزﺎﺳ . ﻻ ﺎﻣا ﯾ ﺘﭙﭘﻮﮐﻮﻣ ﻪ ﯿ ﺪ ي

ﺮﺘﮐﺎﺑ رد هدﺎﺳ ي

ﺎﻫ ي اراد ﺖﺒﺜﻣ مﺮﮔ ي

ﺬﭘذﻮﻔﻧ ﯾﺮ ي ﻻﺎﺑ ﯾ ﯽ ﻣ ﯽ -

ﺳﺎﺴﺣ ﻪﮐ ﺪﺷﺎﺑ ﯿ

ﺑ ﺖ ﯿ نآ ﺮﺘﺸ ﮐﺮﺗ ﻪﺑ ﺖﺒﺴﻧ ﺎﻫ ﯿ

ﺮﮕﺑآ تﺎﺒ ﯾ ﻪﺑ ار ﺰ

دراد لﺎﺒﻧد ] 12 , 19 [.

3 - 2 - اﻮﺘﺤﻣ ي ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ﯿ

ﻞﮐ ﺪ

اﻮﺘﺤﻣ ي ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻞﮐ لﻮﻨﻓ ﯽ

ﮔ ﯿ ﺎﺑ ﺮﺑاﺮﺑ كزار هﺎ mg

GAE/g 47 / 96 اﻮﺘﺤﻣ و ي ﺋﻮﻧوﻼﻓ ﯿ ﺎﺑ ﺮﺑاﺮﺑ ﺎﺑ ﺮﺑاﺮﺑ نآ ﺪ mg

50QE/g / 28 دﻮﺑ ) ﻞﮑﺷ 3 .(

ﮑﺴﮐ ﯿ نارﺎﮑﻤﻫ و ﻦ ]

20 [ شراﺰﮔ

ﻣ ﻪﮐ ﺪﻧدﻮﻤﻧ ﯿ

گﺮﺑ و جﺎﮐ هﻮ ﺎﻫ

ي ﺗﺮﺗ ﻪﺑ كزار ﯿ

اراد ﺐ ي اﻮﺘﺤﻣ ي

ﻞﮐ لﻮﻨﻓ mg GAE/g 12

/7 و 86 /6 ﻣ ﯽ ﺪﺷﺎﺑ . ا ﺮﺑ هوﻼﻋ ﯾ ،ﻦ

ﻣﯿ ﻞﮐ لﻮﻨﻓ ناﺰ mg GAE/g

15 رد كزار گﺮﺑ هرﺎﺼﻋ رد

Microorganism MIC (mg/mL) MBC (mg/mL)

Enterobacter aerogenes 256 > 512

Escherichia coli 128 > 512

Staphylococcus aureus 64 512

Listeria monocytogenes 128 > 512

(6)

نارﺎﮑﻤﻫ و ماﺮﺑآ ﻪﻌﻟﺎﻄﻣ ]

10 [ ﺖﺳا هﺪﺷ شراﺰﮔ .

ﻨﭽﻤﻫ ﯿ ،ﻦ

ﻟﺎﻣ ﯿ نارﺎﮑﻤﻫ و رﺎ ]

21 [ رﺎﮑﻤﻫ و سﺎﮐرﺎﻓ و نا

] 22 [ اﻮﺘﺤﻣ ي

ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﻞﮐ لﻮﻨﻓ ﯽ

ﺗﺮﺗ ﻪﺑ ار كزار ﯿ

ﺐ μg GAE/g 4

/7

و mg GAE/g 39

/2 ﺪﻧداد شراﺰﮔ .

ﻣﯿ كزار ﻞﮐ لﻮﻨﻓ ناﺰ

ﺛﺄﺗ ﺖﺤﺗ ﯿ ﻔﻠﺘﺨﻣ ﻞﻣاﻮﻋ ﺮ ﯽ

ﺒﻗ زا ﯿ اﺮﺷ ،ﺖﺸﮐ ﻪﻘﻄﻨﻣ ﻞ ﯾ

و بآ ﻂ

اﻮﻫ ﯾ ﯽ شور ، ﺎﻫ ي ﻣ راﺮﻗ لﻼﺣ عﻮﻧ و جاﺮﺨﺘﺳا ﯽ

ﮔﯿ دﺮ ] 10 [ .

ﮔ هرﺎﺼﻋ ﯿ اراد كزار هﺎ ي

ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻔﻠﺘﺨﻣ ﯽ ﺒﻗ زا ﯿ ﻞ

ﭽﺗﺎﮐ ﯿ ﭘا و ﻦ ﯽ ﭽﺗﺎﮐ ﯿ ﻣ ﻦ ﯽ ﺪﺷﺎﺑ . نارﺎﮑﻤﻫ و نﺎﺒﺘﺳا ﻮﺴﻧﻮﻟآ ]9

[

ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﻪﮐ ﺪﻧدﻮﻤﻧ شراﺰﮔ ﯽ

ﮔ ﯿ وﺎﺣ كزار هﺎ ي

7 / 13

ﻣﯿ ﻠ ﯽ ﭽﺗﺎﮐ مﺮﮔ رد مﺮﮔ ﯿ

و ﻦ 9/

3 ﻣ ﯿﻠ ﯽ ﭘا مﺮﮔ رد مﺮﮔ ﯽ

ﭽﺗﺎﮐ ﯿ ﻦ

ﺖﺳا . ﻪﻌﻟﺎﻄﻣ رد ا ي د ﯾ دﺮﮔ ﺺﺨﺸﻣ ﺮﮕ ﯾ

ﻣ ﻪﮐ ﺪ ﯿ ﭽﺗﺎﮐ ناﺰ ﯿ و ﻦ

ﭘا ﯽ ﭽﺗﺎﮐ ﯿ ﻮﻧﺎﺘﻣ هرﺎﺼﻋ رد ﻦ ﻟ

ﯽ ﺗﺮﺗ ﻪﺑ كزار ﯿ

ﺐ 37 /0 و 83 /0

ﻣﯿ ﻠ ﯽ ﻣ مﺮﮔ رد مﺮﮔ ﯽ

ﺪﺷﺎﺑ ] 20 [ . ا ﺮﺑ هوﻼﻋ ﯾ هﺪﺷ شراﺰﮔ ،ﻦ

ﻟﻮﺌﺗﻮﻟ ﻪﮐ ﺖﺳا ﯿ

ﺗور ،ﻦ ﯿ ﻟوﺮﻓ ،ﻦ ﯿ ﺳا ﮏ ﯿ ﭽﺗﺎﮐ و ﺪ ﯿ هﺪﻤﻋ ﻦ ﺮﺗ ﯾ ﻦ

ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﺎﺳﺎﻨﺷ ﯾ ﯽ ﻣ هرﺎﺼﻋ رد هﺪﺷ ﯿ

ﺗور و كزار هﻮ ﯿ

،ﻦ

ﭘا ﯽ ﭽﺗﺎﮐ ﯿ ﻧاو ،ﻦ ﯿﻠﯿ ﺳا ﮏ ﯿ د و ﺪ ﯾ ﺋزدﺎ ﯿ ناواﺮﻓ ﻦ ﺮﺗ ﯾ ﮐﺮﺗ ﻦ ﯿ تﺎﺒ

ﻟﻮﻨﻓ ﯽ رد ﻣ كزار گﺮﺑ هرﺎﺼﻋ ﯽ

ﺪﻨﺷﺎﺑ ] 20 [ . ﺖﺳا ﺮﮐذ ﻪﺑ مزﻻ

ﭽﺗﺎﮐ ﻪﮐ ﯿ ﭘا و ﻦ ﯽ ﭽﺗﺎﮐ ﯿ هدﺮﺘﺴﮔ رﻮﻄﺑ ﻦ ا

ي ﻧدوﺰﻓا ناﻮﻨﻌﺑ ﯽ

اﺬﻏ داﻮﻣ رد اﺮﮕﻠﻤﻋ ﯾ

ﯽ ﻞﻤﮑﻣ و ﺎﻫوراد اﺬﻏ ، ﺎﻫ

ي ﺬﻐﺗ ﯾﻪ ا ي ﻪﺑ

ﮔﻮﻠﺟ ،نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ ﺖﻣﻼﺳ دﻮﺒﻬﺑ رﻮﻈﻨﻣ ﯿﺮ

ي ﺑ زا ﯿ رﺎﻤ ي ﺎﻫ ي

اﺰﻓا و ﻒﻠﺘﺨﻣ ﯾ

راﺪﻬﮕﻧ ﺮﻤﻋ ﺶ ي

ﻣ هدﺎﻔﺘﺳا تﻻﻮﺼﺤﻣ ﯽ

ﺪﻧﻮﺷ

] 23 - 25 [.

Fig 3 Total phenolic content (TPC) and total flavonoid content (TFC) of Humulus lupulus

extract.

3 - 3 - ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ

ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ و ﺶﻘﻧ ﯾ هﮋ ا ي ﻟﺎﻌﻓ رد ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ هرﺎﺼﻋ ﺎﻫ

ﺲﻧﺎﺳا و ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ ا ﯾ ﻣ ﺎﻔ ﯽ ﺪﻨﻨﮐ . ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ هرﺎﺼﻋ ﻟﻮﻧﺎﺗا

ﯽ ﮔ ﯿ دار رﺎﻬﻣ شور ود ﻖﺑﺎﻄﻣ كزار هﺎ ﯾ

لﺎﮑ ﺎﻫ ي دازآ

وDPPH هزاﺪﻧاABTS

ﮔﯿ ﺮ ي ﺎﺘﻧ و ﯾ ﻞﮑﺷ رد ﺞ 4

هﺪﺷ هدروآ

ﺖﺳا . دﺮﮔ هﺪﻫﺎﺸﻣ ﯾ

ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ ﺪ ﯽ

ﮔ ﯿ اراد كزار هﺎ ي

ﻟﺎﻌﻓ ﯿ دار رﺎﻬﻣ ﺖ ﯾ

ﻞﺑﺎﻗ دازآ لﺎﮑ ﻬﺟﻮﺗ

ﯽ ﻣ ﯽ ﺪﺷﺎﺑ . رﻮﻄﺑ ﯾ ﻪﮑ

ﻟﺎﻌﻓ ﯿ ﻣ ﺖ ﮔﺪﻨﻨﮐرﺎﻬ ﯽ دار ﺮﺑاﺮﺑ رد ﯾ

لﺎﮑ ﺎﻫ ي دازآ وDPPH

ﺗﺮﺗ ﻪﺑABTS ﯿ لدﺎﻌﻣ ﺐ 63 / 58 و 5/

66 دﻮﺑ ﺪﺻرد .

ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻪﺑ ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ رد كزار هرﺎﺼﻋ

ﺳر تﺎﺒﺛا ﯿ ﺖﺳا هﺪ . ﻟﺎﻣ ﯿ نارﺎﮑﻤﻫ و رﺎ ]

21 [ ﻪﮐ ﺪﻧداد شراﺰﮔ

ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﯽ

ﮔ ﯿ ﺮﺑاﺮﺑ رد كزار هﺎ

دار ﯾ لﺎﮑ ﺎﻫ ي دازآ وDPPH

ﺗﺮﺗ ﻪﺑABTS ﯿ رد ﺐ

هدوﺪﺤﻣ μgTorolox/g 3

/ 44 - 7/

23 و 6/

60 - 8/

43 ﻣ ﯽ ﺪﺷﺎﺑ .

نارﺎﮑﻤﻫ و ماﺮﺑآ ]

10 [ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ ﺪﻧداد نﺎﺸﻧ ﯽ

گﺮﺑ

اراد كزار ي ﻟﺎﻌﻓ ﯿ دار رﺎﻬﻣ ﺖ ﯾ

دازآ لﺎﮑ ﺮﺘﻤﮐDPPH

ي ﺖﺒﺴﻧ

ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﺑ ﯽ

ﻣ ﯿ ﮔ هﻮ ﯿ ﻣ هﺎ ﯽ ﺪﺷﺎﺑ ) دوﺪﺣ 5 ﺮﺑاﺮﺑ .(

ﻟﺎﻌﻓ ﯿ ﺖ

ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻋ ﻟﻮﻧﺎﺘﻣ هرﺎﺼ ﯽ

ﻣ و گﺮﺑ ﯿ

ﺮﺑاﺮﺑ رد كزار هﻮ

دار ﯾ لﺎﮑ ﺎﻫ ي دازآ )DPPH mg/ml 99

/0 78 – /0 ( رد

ﻪﻌﻟﺎﻄﻣ ي ﮑﺴﮐ ﯿ نارﺎﮑﻤﻫ و ﻦ ]

20 [ ﺖﺳا هﺪﺷ شراﺰﮔ .

هوﻼﻋ

ا ﺮﺑ ﯾ نارﺎﮑﻤﻫ و نﺎﺒﺘﺳا ﻮﺴﻧﻮﻟآ ،ﻦ ]

9 [ ﺑ ﯿ هرﺎﺼﻋ ﻪﮐ ﺪﻧدﻮﻤﻧ نﺎ

ﻟﻮﻧﺎﺘﻣ ﯽ اراد كزار ي

ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻞﺑﺎﻗ ﻬﺟﻮﺗ ﯽ

) μg/ml 505 ( ﺑ رد دار ﺮﺑاﺮ ﯾ دازآ لﺎﮑ درادDPPH

. ﻟﺎﻌﻓ ﯿ ﺖ

ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺷﺎﻧ ًﺎﺗﺪﻤﻋ كزار هرﺎﺼﻋ ﯽ

ﮐﺮﺗ زا ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ و

ﺋﻮﻧوﻼﻓ ﯿ ﺪ ي ﻣ نآ ﯽ رﻮﻄﺑ ؛ﺪﺷﺎﺑ ﯾ

ﺘﻧآ ﺮﺛا ﻪﮑ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﭽﺗﺎﮐ ﯿ و ﻦ

ﭘا ﯽ ﭽﺗﺎﮐ ﯿ ﺖﺳا هﺪﺷ هداد نﺎﺸﻧ ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ رد ﻦ

] 26 - 29 [ .

Fig 4 Antioxidant activity of Humulus lupulus extract.

ا ﺎﺑ ﯾ ﺎﻘﻣ ،لﺎﺤﻨ ﯾ ﺎﺘﻧ ﻪﺴ ﯾ ا ﺞ ﯾ ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦ ﯾ

ﻪﺘﻓﺎ ﺎﻫ ي ﺎﺳ ﯾ نﺎﻘﻘﺤﻣ ﺮ

ﻣ نﺎﺸﻧ ﯽ ﻣ توﺎﻔﺗ ﻪﮐ ﺪﻫد ﯿ

اﺮﺑ هﺪﺷ شراﺰﮔ داﺪﻋا نﺎ ي

ﻟﺎﻌﻓ ﯿ ﺖ

ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ اﺮﺷ ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ كزار هرﺎﺼﻋ ﯾ

و بآ ﻂ

(7)

اﻮﻫ ﯾ ﯽ اﺮﺷ ، ﯾ ﮔ ﺖﺸﮐ ﻂ ﯿ

ﻊﻤﺟ نﺎﻣز ،هﺎ آ

رو ي ﮔ ﯿ شور ،هﺎ

هزاﺪﻧا شور ،لﻼﺣ عﻮﻧ ،جاﺮﺨﺘﺳا ﮔﯿ

ﺮ ي ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ

ﻣ و ﯿ ﮐﺮﺗ ناﺰ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﺋﻮﻧوﻼﻓ و ﯿ

ﺪ ي دﻮﺷ هداد ﺖﺒﺴﻧ

] 30 - 32 [.

4 - ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي ﺎﻬﻧ ﯾ ﯽ

ﺎﺘﻧ ﯾ ا ﺞ ﯾ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﻦ ﯽ

ﮔ ﯿ ﺛﺄﺗ كزار هﺎ ﯿ

ﺮ

ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﻞﺑﺎﻗ ﻬﺟﻮﺗ ﯽ ﺮﺘﮐﺎﺑ عﻮﻧ ود ﺮﻫ ﺮﺑ ي

ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ و

دراد ﺖﺒﺜﻣ مﺮﮔ .

ا ﺎﺑ ﯾ ﺳﺎﺴﺣ ،لﺎﺤﻨ ﯿ

ﺮﺘﮐﺎﺑ ﺖ ي ﺎﻫ ي ﺖﺒﺜﻣ مﺮﮔ

ﻔﻨﻣ مﺮﮔ عاﻮﻧا ﻪﺑ ﺖﺒﺴﻧ ﯽ

دﻮﺑ ﺮﺗﻻﺎﺑ هرﺎﺼﻋ ﻪﺑ ﺖﺒﺴﻧ .

رﻮﻄﺑ ﯾ ﻪﮑ

اﺮﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ي

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﺑ ﺖﺒﺜﻣ مﺮﮔ ﯿ

و دﻮﺑ ﺮﺘﺸ

ﺮﺘﻤﮐ ﺖﻈﻠﻏ ي

ﻟﻮﻧﺎﺗا هرﺎﺼﻋ زا ﯽ

اﺮﺑ كزار ي ﮔﻮﻠﺟ ﯿﺮ ي ﺪﺷر زا

و ﯾ ﺑ زا ﺎ ﯿ ا ندﺮﺑ ﻦ ﯾ ﺎﺑ ﻦ ﺮﺘﮐ ي ﻧ ﺎﻫ ﯿ دﻮﺑ زﺎ . ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﯽ

ﮔ ﯿ هﺎ

اراد كزار ي ﻣ ﯿ ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ناﺰ ﯿ

ﺑﻮﻠﻄﻣ ﻞﮐ ﺪ ﯽ

اﺮﺑﺎﻨﺑ و دﻮﺑ ﯾ

ﻦ

ﻞﺑﺎﻗ رﻮﻄﺑ ﻬﺟﻮﺗ

ﯽ دار رﺎﻬﻣ ﺐﺒﺳ ﯾ

لﺎﮑ ﺎﻫ ي دازآ وDPPH

دﺮﮔABTS ﯾﺪ . ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ،اﺬﻟ ﯽ

ﮔ ﯿ ﻠﺑﺎﻗ كزار هﺎ ﯿ

لﺮﺘﻨﮐ ﺖ

ﺴﺑ ﺪﺷر ﯿ رﺎ ي ﺮﺘﮐﺎﺑ زا ي ﺎﻫ ي ﻨﭽﻤﻫ و نژﻮﺗﺎﭘ ﯿ

ﮔﻮﻠﺟ ﻦ ﯿﺮ ي زا

ﺴﮐا ﯿ ﺳاﺪ ﯿ ﻟ نﻮ ﯿﭙﯿ ﻣ اراد ار ﺎﻫﺪ ﯽ

ﻣ و ﺪﺷﺎﺑ ﯽ ا زا ناﻮﺗ ﯾ ﮐﺮﺗ ﻦ ﯿ ﺐ

زﯾ اﺰﻓا ﺖﻬﺟ لﺎﻌﻓ ﺖﺴ ﯾ

ا ﺶ ﯾ ﻨﻤ ﯽ ﮐ ، ﯿﻔ ﯿ رﺎﮔﺪﻧﺎﻣ ﺮﻤﻋ و ﺖ ي

اﺬﻏ تﻻﻮﺼﺤﻣ ﯾ

ﯽ دﻮﻤﻧ هدﺎﻔﺘﺳا .

5 - ﺪﻘﺗ ﯾ ﺮﮑﺸﺗ و ﺮ

ﻣ مزﻻ دﻮﺧ ﺮﺑ ﻪﻟﺎﻘﻣ نﺎﮔﺪﻨﺴﯾﻮﻧ ﯽ

ﺸﻫوﮋﭘ ﺖﻧوﺎﻌﻣ زا ﺪﻨﻧاد ﯽ

و

روﺎﻨﻓ ي زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ي

ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ

ﯽ ﻪﺑ نﺎﺘﺳزﻮﺧ

ﻟد ﯿ ﺎﻤﺣ ﻞ ﯾ ﺖ ﺎﻫ ي دﺎﻣ ي ﻮﻨﻌﻣ و ي ﻤﺻ ﯿ ﻧادرﺪﻗ و ﺮﮑﺸﺗ ﻪﻧﺎﻤ ﯽ

ﺪﻨﯾﺎﻤﻧ .

6 - ﻊﺑﺎﻨﻣ

[1] R. Kasra Kermanshahi, B. Nasr Esfahani, A. A. Pour babaie, G. Asghari, and J. Esmi Serkani, "The Study of Antibacterial Effect of Humulus lupulus on some of Gram Positive & Gram Negative Bacteria,"

Journal of Medicinal Plants, vol. 8, no. 30, pp. 92-97, 2009.

[2] M. Yeganegi, F. Tabatabaei Yazdi, S. A.

Mortazavi, J. Asili, B. Alizadeh Behbahani, and A. Beigbabaei, "Equisetum telmateia extracts: Chemical compositions, antioxidant activity and antimicrobial effect on the growth of some pathogenic strain causing

poisoning and infection," Microbial Pathogenesis, vol. 116, pp. 62-67, 2018.

[3] P. Namazi, H. Barzegar, B. Alizadeh behbahani, and M. A. Mehrnia, "Evaluation of functional groups of bioactive compounds, antioxidant potential, total phenolic and total flavonoid content of red bell pepper extracts," Food Science and Technology, vol.

18, no. 113, pp. 301-311, 2021.

[4] H. Barzegar, B. Alizadeh behbahani, and M. Noshad, "Evaluation of total phenol and flavonoid contents, antioxidant and antimicrobial activity of Lawsonia inermis aqueous extract against some gram- positive and gram- negative bacteria," Food Science and Technology, vol. 18, no. 116, pp. 327- 335, 2021.

[5] F. Tabatabaei Yazdi and B. Alizadeh Behbahani, "Antimicrobial effect of the aqueous and ethanolic Teucrium polium L.

extracts on gram positive and gram negative bacteria “in vitro”," Archives of Advances in Biosciences, vol. 4, no. 4, 2013.

[6] B. A. Behbahani, F. T. Yazdi, A.

Mortazavi, F. Zendeboodi, and M. M.

Gholian, "Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis Lon food infection and intoxication microorganisms “in vitro”" Archives of Advances in Biosciences, vol. 4, no. 3, 89-99, 2013.

[7] A. Mohamadi Sani, M. Azami, and M.

Yavarmanesh, "Evaluation of antimicrobial effect of Hop water extract with Humulune from Hop against Staphylococcus aureus, Enterobacter aerogenes, Listeria monocytogenes and Salmonella enterica," (in en), Iranian Food Science and Technology Research Journal, vol. 11, no. 2, pp. 218- 224, 2015.

[8] S. H. Hejazian and S. M. Mahdavi,

"Comparison Between the Analgesic Effect of Humulus Lupulus (Hops) Extract and Morphine in Mice," The Journal of Rafsanjan University of Medical Sciences, vol. 5, no. 4, pp. 265-272, 2006.

[9] J. I. Alonso-Esteban et al., "Phenolic composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) Seeds," Industrial Crops and Products, vol. 134, pp. 154-159, 2019.

[10] V. Abram et al., "A comparison of antioxidant and antimicrobial activity between hop leaves and hop cones,"

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Industrial Crops and Products, vol. 64, pp.

124-134, 2015.

[11] M. Noshad, B. Alizadeh Behbahani, and S. Dehghani, "Evaluation of the effect of aqueous and ethanolic extraction methods on extraction yield, phenolic compounds, and antioxidant and antimicrobial activity of Stachys schtschegleevii extract," Food Science and Technology, vol. 17, no. 100, pp. 117-125, 2020.

[12] H. Zanganeh, S. A. Mortazavi, F. Shahidi, and B. Alizadeh Behbahani, "Evaluation of the chemical and antibacterial properties of Citrus paradise essential oil and its application in Lallemantia iberica seed mucilage edible coating to improve the physicochemical, microbiological and sensory properties of lamb during refrigerated storage," Journal of Food Measurement and Characterization, vol. 15, no. 6, pp. 5556-5571, 2021.

[13] B. Majdi, M. A. Mehrnia, H. Barzegar, and B. Alizadeh Behbahani, "Determination of the structure, chemical composition, antioxidant activity and the cytotoxic effect of Turmeric essential oil," (in en), Iranian Food Science and Technology Research Journal, vol. 17, no. 2, pp. 261-271, 2021.

[14] M. Rahmati-Joneidabad and B. Alizadeh behbahani, "Evaluation of chemical activity and antifungal effect of Vitex agnus-castus essential oil on Penicillium digitatum and Penicillium italicum causing orange rot,"

mdrsjrns, vol. 18, no. 114, pp. 82-73, 2021.

[15] F. Shahidi, F. Tabatabaei Yazdi, B.

Alizadeh Behbahani, S. Roshanak, N.

Norouzi, A. Vasiee, "Aantibacterial Effect of Tragopogon graminifolius Extract on Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli and Salmonella typhi “in vitro”," Iranian Journal of Infectious Diseases and Tropical Medicine, vol. 24, no. 84, pp. 1-10, 2019.

[16] M. Ebrahimi Hemmati Kaykha, H.

Jooyandeh, B. Alizadeh Behbahani, M.

Noshad, "Antimicrobial Activity of Rosemary Essential Oil and its Interaction with Common Therapeutic Antibiotics on some Gram Positive and Gram Negative Bacteria," Journal of Infectious Diseases and Tropical Medicine, vol. 87, pp. 25-34, 2020.

[17] M. Rahmati-Joneidabad, B. Alizadeh Behbahani, and M. Noshad, "Antifungal effect of Satureja khuzestanica essential oil on Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer causing strawberry’s rot

and mold," Food Science and Technology, vol. 18, no. 115, pp. 171-180, 2021.

[18] F. M. S. Gomes et al., "Evaluation of antibacterial and modifying action of catechin antibiotics in resistant strains,"

Microbial Pathogenesis, vol. 115, pp. 175- 178, 2018.

[19] H. Zanganeh, F. Shahidi, S. A. Mortazavi, and B. Alizadeh Behbahani, "Evaluation of antioxidant and antimicrobial properties of Citrus paradisi essential oil nanoemulsion on pathogenic microorganisms: A laboratory study," (in en), Iranian Food Science and Technology Research Journal, 2021, doi:

10.22067/ifstrj.2021.71660.1074.

[20] Ş. Keskin, Y. Şirin, H. E. Çakir, and M.

Keskin, "An investigation of Humulus lupulus L.: Phenolic composition, antioxidant capacity and inhibition properties of clinically important enzymes," South African Journal of Botany, vol. 120, pp. 170- 174, 2019.

[21] T. Maliar et al., "Secondary metabolites, antioxidant and anti-proteinase activities of methanolic extracts from cones of hop (Humulus lupulus L.) cultivars," Chemical Papers, vol. 71, no. 1, pp. 41-48, 2017.

[22] A. Fărcaş, M. Tofană, S. Socaci, S. Scrob, L. Salanţă, and A. Borşa, "Preliminary study on antioxidant activity and polyphenols content in discharged waste from beer production," J. Agroaliment. Process.

Technol, vol. 19, pp. 319-324, 2013.

[23] V. K. Ananingsih, A. Sharma, and W.

Zhou, "Green tea catechins during food processing and storage: A review on stability and detection," Food Research International, vol. 50, no. 2, pp. 469-479, 2013.

[24] A. F. Massounga Bora, S. Ma, X. Li, and L. Liu, "Application of microencapsulation for the safe delivery of green tea polyphenols in food systems: Review and recent advances," Food Research International, vol.

105, pp. 241-249, 2018.

[25] M. Karabin, T. Hudcova, L. Jelinek, and P. Dostalek, "Biotransformations and biological activities of hop flavonoids,"

Biotechnology Advances, vol. 33, no. 6, Part 2, pp. 1063-1090, 2015.

[26] T. Inui, K. Okumura, H. Matsui, T.

Hosoya, and S. Kumazawa, "Effect of harvest time on some in vitro functional properties of hop polyphenols," Food Chemistry, vol. 225, pp. 69-76, 2017.

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[27] M. Grzesik, K. Naparło, G. Bartosz, and I. Sadowska-Bartosz, "Antioxidant properties of catechins: Comparison with other antioxidants," Food Chemistry, vol. 241, pp.

480-492, 2018.

[28] I. Peres, S. Rocha, J. Gomes, S. Morais, M. C. Pereira, and M. Coelho, "Preservation of catechin antioxidant properties loaded in carbohydrate nanoparticles," Carbohydrate Polymers, vol. 86, no. 1, pp. 147-153, 2011.

[29] A. A. Zanwar, S. L. Badole, P. S. Shende, M. V. Hegde, and S. L. Bodhankar, "Chapter 21 - Antioxidant Role of Catechin in Health and Disease," in Polyphenols in Human Health and Disease, R. R. Watson, V. R.

Preedy, and S. Zibadi Eds. San Diego:

Academic Press, 2014, pp. 267-271.

[30] Z. Sosani Gharibvand, B. Alizadeh Behbahani, M. Noshad, and H. Jooyandeh,

"Investigation of the Functional Groups of Bioactive Compounds, Radical Scavenging Potential, Antimicrobial Activity and

Cytotoxic Effect of Callistemon Citrinus Aqueous Extract on Cell Line HT29: A Laboratory Study," The Journal of Rafsanjan University of Medical Sciences, vol. 19, no.

5, pp. 463-484, 2020.

[31] F. Falah, K. Shirani, A. Vasiee, F.

Tabatabaee Yazdi, and B. Alizadeh Behbahani, "In vitro screening of phytochemicals, antioxidant, antimicrobial, and cytotoxic activity of Echinops setifer extract," Biocatalysis and Agricultural Biotechnology, vol. 35, p. 102102, 2021.

[32] M. A. Mehrnia, B. Alizadeh Behbahani, H. Barzegar, and H. Tanavar, "Sclerorhachis platyrachis essential oil: Antioxidant power, total phenolic and flavonoid content and its antimicrobial activity on some Gram-positive and Gram-negative bacteria “in vitro”," Food Science and Technology, vol. 18, no. 112, pp. 189-198, 2021.

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Investigation of the chemical properties and antimicrobial activities of Humulus lupulus extract on Staphylococcus aureus,

Listeria monocytogenes, Escherichia coli, and Enterobacter aerogenes in vitro

Noshad, M. ^1*, Alizadeh Behbahani, B. ², Rahmati-Joneidabad, M. ³

1. Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

2. Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

3. Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

ABSTRACT ARTICLE INFO

Article History:

Received 2022/ 12/ 24 Accepted 2022/ 02/ 22

Keywords:

Humulus lupulus extract, Antioxidant,

Antimicrobe, Pathogenic bacteria, Natural preservative.

In this study, the ethanolic extract of Humulus lupulus was prepared by the maceration method. Its antimicrobial effect was evaluated against pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Enterobacter aerogenes) through disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration. Total phenol and flavonoid contents of the extract were measured by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant activity of the ethanolic extract was also measured by DPPH and ABTS free radical scavenging methods. The highest and lowest inhibition zones in disk diffusion agar (16.9 mm vs. 12.5 mm) and well diffusion agar (17.6 mm vs. 13.9 mm) methods were accounted for S. aureusand E. aerogenes, respectively. Gram-positive bacterial species were more sensitive to H. lupulus ethanolic extract compared to Gram-negative ones. Total phenol and flavonoid contents of the extract were found to be 96.47 mg GAE/g and 28.5 mg QE/g, respectively. The antioxidant effects of the H. lupulus ethanolic extract, based on DPPH and ABTS radical scavenging activities, were 58.63% and 66.5%, respectively.

Based on the results, the ethanolic extract of H. lupulus could be used as a natural antimicrobial and antioxidant agent to inhibit the growth of pathogenic bacteria and lipid oxidation progression in various food products.

DOI: 10.22034/FSCT.19.126.143

DOR: 20.1001.1.20088787.1401.19.126.15.6

*Corresponding Author E-Mail:

[email protected]

Iranian Journal of Food Science and Technology

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