ﺛﺎﺗ ﯿ د دﺎﻤﺠﻧا نﺎﻣز تﺪﻣ ﺮ اﺰﻓا ر
ﯾ ﻫدزﺎﺑ ﺶ ﯽ
ﻫ ﯿ ﻟورﺪ ﯿ ﺳ ﻪﺘﺳﺎﺸﻧ ﺰ ﯿ
ﻣز ﺐ ﯿﻨ ﯽ ﻣآ ﺎﻔﻟآ ﻂﺳﻮﺗ ﯿ
زﻼ
ﺪﺑﺎﻋ ﻪﻬﻟا
1ي ﮐ ،*
ﯿ ﺪﻤﺤﻣرﻮﭘ ﺎﻧﺎ
1ي
1 - ﺸﻧاد ﯿ ﺎﻨﺻ و مﻮﻠﻋ ﺶﺨﺑ رﺎ ﯾ
اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ هﺪﮑﺸﻧاد ، ي
ﺎﺴﻓ هﺎﮕﺸﻧاد ،
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 16 / 04 / 1401
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 18 / 05 / 1401
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﻣز ﺐ ﯿﻨ ﯽ
،
ﭘﯿ ﺗ ﺶ ﯿ ،دﺎﻤﺠﻧا رﺎﻤ
ﺮﮐ ﺪﺻرد ﯾ
،نﺪﺷ ﻪﻟﺎﺘﺴ ژﻮﻟﻮﻓرﻮﻣ صاﻮﺧ ي
.
DOI: 10.22034/FSCT.19.128.183
DOR: 20.1001.1.20088787.1401.19.128.27.2
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ : e,[email protected]
ﺘﺳد رﻮﻈﻨﻣ ﻪﺑ ﯿﺑﺎ
ﯽ ﻫ ﻪﺑ ﯿ ﻟورﺪ ﯿ ﻂﺳﻮﺗ ﻻﺎﺑ نﺎﻣﺪﻧار ﺎﺑ ﻪﺘﺳﺎﺸﻧ ﺰ ﻣآ-α
ﯿ ﭘ ،زﻼ ﯿ ﺗ ﺶ ﯿ رﺎﻤ
ﺳ ﻪﺘﺳﺎﺸﻧدﺎﻤﺠﻧا ﯿ
ﻣز ﺐ ﯿﻨ ﯽ ﺎﻣد رد ي C 25° - نﺎﻣز ود رد 4
(PF4) و 8 زور (PF8)
ﺖﻓﺮﮔ مﺎﺠﻧا .
اﺮﺑ ي ا ﯾ ﺳ ﻪﺘﺳﺎﺸﻧ ،رﻮﻈﻨﻣ ﻦ ﯿ
ﺐ ﻣز ﯿﻨ ﯽ (NPS) ﭘ و ﯿ ﺗ ﺶ ﯿ راﺮﻗ ﺎﺑ دﺎﻤﺠﻧا ﺎﺑ رﺎﻤ
آ ضﺮﻌﻣ رد ﻦﺘﻓﺮﮔ ﻣآ ﺎﻔﻟ
ﯿ زﻼ (0.15% w/v) اﺮﺑ
ي تﺪﻣ 10 ﺎﻣد رد ﺖﻋﺎﺳ ي
C 25°
مﺮﮔ ﻪﻧﺎﺧ راﺬﮔ ي ﺪﻧﺪﺷ . ژﻮﻟﻮﻓرﻮﻣ صاﻮﺧ ﯾ
ﮑ ﯽ ﻫ ﺪﺻرد ، ﯿ
ﻟورﺪ ﯿ ﺮﮐ ﻪﺟرد ،ﺰ ﯾ
نﺪﺷ ﻪﻟﺎﺘﺴ
ا ﻪﻌﺷا شاﺮﭘ ﻂﺳﻮﺗ ﯾ
ﺲﮑ (X-ray) ﻤﺧ و
ﯿﺮ ي و رآ هﺎﮕﺘﺳد ﻂﺳﻮﺗ نﺪﺷ ي
ا ي (Rapid
visco analyzer; RVA) ﻪﺘﺳﺎﺸﻧ رد
ﺎﻫ ي ﺒﻃ ﯿﻌ ﯽ ﻪﺘﺳﺎﺸﻧ ، ﺎﻫ ي ﭘ ﯿ ﺗ ﺶ ﯿ ﺑ رﺎﻤ و دﺎﻤﺠﻧا ﺎ
ﻪﺘﺳﺎﺸﻧ ﺎﻫ ي ﻫ ﯿ ﻟورﺪ ﯿ ﻣآ ﻂﺳﻮﺗ هﺪﺷ ﺰ ﯿ
ﺖﻓﺮﮔ راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ زﻼ .
ﺳآ ﺚﻋﺎﺑ دﺎﻤﺠﻧا ﯿ
ﻪﺑ ﺐ
ﺎﺘﻧ ﻖﺒﻃ و هﺪﺷ ﻪﺘﺳﺎﺸﻧ لﻮﻧاﺮﮔ ﺢﻄﺳ ﯾ
ژﻮﻟﻮﻓرﻮﻣ زا ﻞﺻﺎﺣ ﺞ ي
ﮕﺘﻓر ﻮﺗ ،شاﺮﺧ ، ﯽ
كﺮﺗ و
ﮔدرﻮﺧ ﯽ ﺪﺷ هﺪﻫﺎﺸﻣ لﻮﻧاﺮﮔ ﺢﻄﺳ رد .
ﺘﻧ رد ﯿ ﻪﺘﺳﺎﺸﻧ ﻪﺠ ﺘﺣار ﻪﺑ ﺎﻫ
ﯽ ﻫ ﺖﺤﺗ ﯿ ﻟورﺪ ﯿ ﻂﺳﻮﺗ ﺰ
ﻣآ -α ﯿ راﺮﻗ زﻼ ﺷﺎﭘوﺮﻓ ﺚﻋﺎﺑ و ﺪﻨﺘﻓﺮﮔ ﯽ
ﺑ ﯿ لﻮﻧاﺮﮔ ﺮﺘﺸ ﺎﻫ
ي ﭘ ﻪﺘﺳﺎﺸﻧ ﯿ ﺗ ﺶ ﯿ ﺎﺑ هﺪﺷ رﺎﻤ
ﺒﻃ ﻪﺘﺳﺎﺸﻧ ﻪﺑ ﺖﺒﺴﻧ دﺎﻤﺠﻧا ﯿﻌ
ﯽ ﺪﺷ . ﺎﺘﻧ ﯾ ﻫ ﺪﺻرد زا ﻞﺻﺎﺣ ﺞ ﯿ
ﻟورﺪ ﯿ دﺎﻤﺠﻧا ﻪﮐ داد نﺎﺸﻧ ﺰ
ﻟﻮﺗ ﺚﻋﺎﺑ ﯿ ﺰﺟ ﺪﺻرد ﺪ ﯾ
ﯽ ﻟﻮﺗ رد ﯿ ﻫ ﻪﺘﺳﺎﺸﻧ ﺪ ﯿ
ﻟورﺪ ﯿ ﺪﺷ ﺰ . ﻫ ﺪﺻرد ﯿ ﻟورﺪ ﯿ ﺰ (%) ﻪﺘﺳﺎﺸﻧ
ﺒﻃ ﯿﻌ ﯽ ﺗ ﺖﺤﺗ ، ﯿ رد دﺎﻤﺠﻧا رﺎﻤ و PF4
ﺑ PF8 ﺗﺮﺗ ﻪ ﯿ ﻪﺑ ﺐ 8 / 16
% ، 1 / 24 % و 8 / 40 % اﺰﻓا ﯾ ﺶ
ﯾ ﺖﻓﺎ . ا ﺮﺑ هوﻼﻋ ﯾ ﺮﮐ ﻪﺟرد ،ﻦ ﯾ
ﺒﻃ ﻪﺘﺳﺎﺸﻧ زا دﺎﻤﺠﻧا زا ﺲﭘ نﺪﺷ ﻪﻟﺎﺘﺴ ﯿﻌ
ﯽ ) 7 / 37 (%
ﻪﺑ
8 / 34 % رد و PF4 2 / 33
% رد ﺶﻫﺎﮐ PF8 ﯾ ا ﻪﮐ ﺖﻓﺎ ﯾ ﻣ ﻦ ﯿ ﺗ زا ﺲﭘ ﺶﻫﺎﮐ ناﺰ ﯿ
رﺎﻤ
ﺎﺑ ﻣآ -α ﯿ ﺴﺑ زﻼ ﯿ تﺪﺷ رﺎ ﯾ ﺒﻃ ﻪﺘﺳﺎﺸﻧ رد و ﺖﻓﺎ ﯿﻌ
ﯽ ﭘ و ﯿ ﺗ ﺶ ﯿ رد رﺎﻤ 4 و زور 8 دﺎﻤﺠﻧا زور
ﺗﺮﺗ ﻪﺑ ﯿ ﻪﺑ ﺐ 3
%/
34 ، 4
%/
29 و 5 / 25 % ﺶﻫﺎﮐ ﯾ ﺖﻓﺎ . ﺎﻣد ي ﺗﻻژ ﯿ ﺮﺜﮐاﺪﺣ و نﺪﺷ ﻪﻨ
وﯾ زﻮﮑﺴ ﯾ ﺗ زا ﺲﭘ ﻪﺘ ﯿ
ﻣآ ﺎﺑ رﺎﻤ ﯿ ﻣﺎﻤﺗ رد زﻼ ﯽ
ﻪﺘﺳﺎﺸﻧ ﺎﻫ ي ﺒﻃ ﯿﻌ ﯽ ﺗ ﺖﺤﺗ و ﯿ
ﻪﺑ دﺎﻤﺠﻧا رﺎﻤ
رادﺎﻨﻌﻣ ترﻮﺻ ي
(p˂ 0.05) ﺶﻫﺎﮐ
ﯾ ﺗﺮﺗ ﻪﺑ ﺶﻫﺎﮐ ﺪﻧور و ﺖﻓﺎ ﯿ
ﺐ PF8˂PF4˂P
ﻟد ﻪﮐ ﺪﺷ هﺪﻫﺎﺸﻣ ﯿ
ا ﻞ ﯾ ﺎﺘﻧ ﻦ ﯾ ﻣ ﺞ ﯽ ﺮﺨﺗ ﺪﻧاﻮﺗ ﯾ ﺎﻫرﺎﺘﺧﺎﺳ ﺐ ي
ﻟﻮﻧاﺮﮔ ﯽ ﻟﻮﮑﻟﻮﻣ نورد و ﯽ
و
ﻨﭽﻤﻫ ﯿ ﺎﭘ مرﻮﺗ ترﺪﻗ ﻦ ﯾﯿ
ﺪﺷﺎﺑ ﻦ .
ﻪﻠﺠﻣ مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣ ﺖﯾﺎﺳ
ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ
[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
1 - ﻪﻣﺪﻘﻣ
ﻪﺘﺳﺎﺸﻧ ﺮﺘﻤﻬﻣ ﯾ ﻦ ﻊﺒﻨﻣ ژﺮﻧا ي ﺧذ ﯿ هﺮ ا ي رد ﮔ ﯿ نﺎﻫﺎ ﻪﺑ رﺎﻤﺷ
ﻣ ﯽ دور ﻪﮐ ﻪﺑ رﻮﻓو رد ﻪﻧاد تﻼﻏ ﺪﻨﻧﺎﻣ )
،مﺪﻨﮔ
،ترذ ﺞﻧﺮﺑ و
ﭘﺎﺗ ﯿ ﺎﮐﻮ ( و رد ﮔ ﯿ نﺎﻫﺎ هﺪﻏ ا ي ﺪﻨﻧﺎﻣ) عاﻮﻧا ﺳ ﯿ ﺐ ﻣز ﯿﻨ ﯽ ( ﯾ ﺖﻓﺎ
ﻣ ﯽ دﻮﺷ . وﯾ ﮔﮋ ﯽ ﺎﻫ ي ﺮﺼﺤﻨﻣ دﺮﻔﺑ ﻪﺘﺳﺎﺸﻧ ﺚﻋﺎﺑ هﺪﺷ ﺖﺳا ﺎﺗ
ناﻮﺘﺑ زا اﯾ ﻦ ﮐﺮﺗ ﯿ ﺐ ﻠﭘ ﯿ ﺮﻤ ي ﻪﺑ ترﻮﺻ هدﺮﺘﺴﮔ رد ﺎﻨﺻ ﯾ ﻊ
ﻒﻠﺘﺨﻣ هدﺎﻔﺘﺳا دﻮﻤﻧ ﻪﮐ رد اﯾ ﻦ ﺑﯿ
،ﻦ ﺖﻌﻨﺻ اﺬﻏ ﻢﻬﺳ هﺪﻤﻋ ا ي
ار ﻪﺑ دﻮﺧ صﺎﺼﺘﺧا هداد ﺖﺳا ]1 .[
ﻪﺑ ناﻮﻨﻋ لﺎﺜﻣ ﻪﺘﺳﺎﺸﻧ ﺎﻫ رد
ﻟﻮﺗ ﯿ ﺪ ﺎﻫردﻮﭘ ي اﻮﻧﺎﻧ ﯾ ﯽ و داﻮﻣ دﻮﺒﻬﺑ هﺪﻨﻫد ﺖﺨﭘ ﻪﺑ ناﻮﻨﻋ
هﺪﻨﻨﮐﺮﭘ هدﺎﻔﺘﺳا ﻣ ﯽ ﺪﻧﻮﺷ . رد ﻟﻮﺗ ﯿ ﺪ عاﻮﻧا ﺲﺳ ﻪﺑ رﻮﻈﻨﻣ ﻆﻔﺣ
ﺴﻟﻮﻣا ﯿ نﻮ ﻦﻏور و ﻪﮐﺮﺳ و ﮔﻮﻠﺟ ﯿﺮ ي زا ود زﺎﻓ نﺪﺷ ﺲﺳ
دﺮﺑرﺎﮐ ﺪﻧراد . ﻠﺑﺎﻗ ﯿ ﺖ د ﯾ ﺮﮕ نآ ﺎﻫ دﻮﺒﻬﺑ ﺖﻓﺎﺑ ﺑﯿ ﻮﮑﺴ ﯾ ﺖ و ﺮﮐاﺮﮐ
و دﺮﺗ ي ﺎﻬﻧآ و لﺮﺘﻨﮐ ﺖﺳا pH
. رد ﺎﻨﺻ ﯾ ﻊ ﺖﺨﭘ ﮐ ﯿ ﮏ و
،ﻪﭼﻮﻠﮐ رد ز نﺎﻣ ﺐﻟﺎﻗ ﮔﯿ ﺮ ي
، اﺮﺑ ي ﮔﻮﻠﺟ ﯿﺮ ي زا ﺒﺴﭼ ﯿ نﺪ ﻤﺧ ﯿﺮ
ﻪﺑ ﺐﻟﺎﻗ زا ﻪﺘﺳﺎﺸﻧ هدﺎﻔﺘﺳا ﻣ ﯽ دﻮﺷ . ﻨﭽﻤﻫ ﯿ ﻦ زا ﻪﺘﺳﺎﺸﻧ ﺎﻫ
ﻣ ﯽ ناﻮﺗ رد ﺎﺳ ﯾﺮ ﺎﻨﺻ ﯾ ﻊ ﺪﻨﻧﺎﻣ زﺎﺳوﺮﺴﻨﮐ ي
، ﺖﺷﻮﮔ و ﺎﻫاﺬﻏ ي
ﺪﻤﺠﻨﻣ هدﺎﻔﺘﺳا دﻮﻤﻧ . وﯾ ﮔﮋ ﯽ ﺎﻫ ي ﻓ ﯿﺰ ﯾ ﺷﻮﮑ ﯿﻤ ﯿﺎﯾ ﯽ ﻪﺘﺳﺎﺸﻧ و
ﺎﻫدﺮﺑرﺎﮐ ي نآ رد ﺎﻨﺻ ﯾ ﻊ ﻒﻠﺘﺨﻣ ﺖﺤﺗ ﺛﺎﺗ ﯿﺮ رﺎﺘﺧﺎﺳ نآ زا
ﻪﻠﻤﺟ ﻣ ﯿ ناﺰ ﺮﮐ ﯾ ﻪﻟﺎﺘﺴ
،ندﻮﺑ ﺖﺒﺴﻧ ﻣآ ﯿ زﻮﻠ ﻪﺑ ﻣآ ﯿ ﺘﮑﭘﻮﻠ ﯿ
،ﻦ
ژﻮﻟﻮﻓرﻮﻣ ي لﻮﻧاﺮﮔ و لﻮﻧاﺮﮔﺮﻄﻗ ﺖﺳﺎﻫ ] 3 - 1 .[
اﺮﺑﺎﻨﺑ ﯾ ﻦ
ﺑ صاﻮﺧ ندﻮﺑ اراد ﺎﺑ ﻪﺘﺳﺎﺸﻧ ﯽ
ﻈﻧ ﯿﺮ ي لژ نﻮﭼ زﺎﺳ ي ماﻮﻗ ،
ﮔﺪﻨﻫد ﯽ ﮔﺪﻨﻨﮐﺮﭘ ، ﯽ اﺬﻏ ﺖﻌﻨﺻ رد ه،
ﯾ ﯽ وراد ، ﯾ ﯽ ﺷ و ﯿﻤ ﯿﺎﯾ ﯽ
ﺴﺑ دﺮﺑرﺎﮐ ﯿ رﺎ ي دراد . ا هزوﺮﻣا ﯾ دﺮﮑﻠﻤﻋ صاﻮﺧ ﻦ ي
ﺎﺑ ﻪﺘﺳﺎﺸﻧ
ﺷ تﺎﺣﻼﺻا لﺎﻤﻋا ﯿﻤ
ﯿﺎﯾ ﯽ ﻓ ، ﯿﺰ ﯾ ﮑ ﯽ ﯾ ﺰﻧآ ﺎ ﯾﻤ ﯽ ﭘ دﻮﺒﻬﺑ ﯿ هدﺮﮐ اﺪ
ﺖﺳا ] 3 - 1 .[
ﻣآ ﺎﻔﻟآ ﯿ اﺮـﺑ ﻪﺘﺳﺎﺸـﻧ ﺖﻌﻨﺻ رد ﺐﻠﻏا ﺎﻫزﻼ ي
ﻟﻮﺗ ـﯿ ﺑ ﺪ ﯿ ،لﻮﻧﺎـﺗاﻮ
ﻻﺎـﺑ زﻮـﺘﮐوﺮﻓ ﺎﺑ ﺖﺑﺮﺷ ـﯾ
ﻟا ،ﺰﮐﻮـﻠﮔ ﺎ ﯿ
رﺎﮐﺎـﺳﻮﮕ ﯾ زﻮﺘﻟﺎـﻣ و ﺎﻫﺪ
ﻣ هدﺎﻔﺘﺳا ﯽ دﻮﺷ . ا زا ﯾ ﮐﺮﺗ ﻦ ﯿ ﺷﻮﻧ و اﺬﻏ رد ًﺎﺗﺪﻤﻋ تﺎﺒ ﯿ
ﻧﺪ ﯽ ﺎـﻫ ي
ﻤﺨﺗ ﯿﺮ ﯾ ﻪﺒ ﺎﺟ ناﻮﻨﻋ ﯾ ﺰﮕ ﯾﻨ ﯽ اﺮﺑ ي ـﺑﺮﭼ و ﺮﮑـﺷ ﯽ
ـﯾ ناﻮـﻨﻋ ﻪـﺑ ﺎ
هﺪﻧراﺪﻬﮕﻧ ﺎﻫ ي ﻋﻮﻨﺼﻣ ﯽ ﺑ ﺪﺿ ﻞﻣاﻮﻋ و ﯿﺗﺎ
ﯽ ـﻣ هدﺎﻔﺘﺳا ﯽ دﻮـﺷ .
ا ﺮﺑ هوﻼﻋ ﯾ ا ،ﻦ ﯾ ﮐﺮﺗ ﻦ ﯿ ﻫ زا ﻞﺻﺎﺣ تﺎﺒ ﯿ
ﻟورﺪ ﯿ ﻣآ ﺎﻔﻟآ ﺰ ﯿ اﺮﺑ ،زﻼ ي
ﻈﻨﺗ ﯿ اﺰﻓا ﻢ ﯾ ﻟﻮﺴﻧا ﺖﻈﻠﻏ ﺶ ﯿ
فﺮـﺻ زا ﺲـﭘ نﻮـﺧ ﺰﮐﻮﻠﮔ و ﻦ ﻧ و ،اﺬﻏ
ﯿ ﻈﻨﺗ ﺰ ﯿ ﻣ رﺎﮑﺑ ﻪﺘﺳﺎﺸﻧ ﻢﻀﻫ ﺖﻋﺮﺳ ﻢ ﯽ
و ﺪﻧور دﺎﻘﻣ ﯾﺮ ي
ژر رد نآ زا ﯾ
اﺬﻏ ﻢ ﯾ ﯽ ﺎﺑ نﺎﺴﻧا ﯾ ﻪـﺘﻓﺮﮔ ﺮـﻈﻧ رد ﻊـﻄﻗ رﻮـﻃ ﻪﺑ ﺪ
دﻮﺷ . ا ﺮﺑ دﺎﻘﺘﻋا ﯾ زا ﺲـﭘ نﻮﺧ ﺪﻨﻗ تﺎﻧﺎﺳﻮﻧ ﺶﻫﺎﮐ ﻪﮐ ﺖﺳا ﻦ
ﺻﺎﺧ تاﺮﺛا ،اﺬﻏ ﯽ
ﺘﻣﻼﺳ ﺮﺑ ﯽ ﻪﺑ وﯾ ﭘ ﺮﻈﻧ زا هﮋ ﯿ
ﮕﺸ ﯿﺮ ي نﺎﻣرد و
دﯾ دراد ﺖﺑﺎ . ﻧ تﺎﻌﻟﺎﻄﻣ ﯿ ﮐﺎﺣ ﺰ ﯽ ﻣآ ﺎﻔﻟآ ﺮﺛا زا ﯿ
اﺮﺑ ﺎﻫزﻼ ي ﺶﻫﺎﮐ
ﮕﺘﺴﺧ ﯽ ﮔﻮﻠﺟ ، ﯿﺮ ي زا ﯾ ﺖﺳﻮﺒ شراﺰـﮔ هدور دﺮـﮑﻠﻤﻋ دﻮﺒﻬﺑ و
هدﺮﮐ ﺪﻧا ] 4 .[
ناﻮـﻨﻋ ﻪـﺑ دﺎﻤﺠﻧا ﯾ
ـﮑ ﯽ شور زا ﺎـﻫ ي ﻓ ﯿﺰ ﯾ ـﮑ ﯽ اﺮـﺑ ي حﻼـﺻا
ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﻪﺘﺳﺎﺸﻧ .
ﻣﺎﮕﻨﻫ ﯽ لﻮﻧاﺮﮔ ﻪﮐ ﺎﻫ
ي رد ﻪﺘﺳﺎﺸـﻧ
ــﻣ راﺮــﻗ دﺎــﻤﺠﻧا ضﺮــﻌﻣ ﯽ
ﮔﯿ ر ،ﺪــﻧﺮ ﯾ صاﻮــﺧ و رﺎﺘﺧﺎــﺳﺰ
ﻓﯿ ﺰﯾ ﺷﻮﮑ ﯿﻤ ﯿﺎﯾ ﯽ هدﺮﺘﺴﮔ رﻮﻃ ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ ا
ي ﻐﺗ ﯿﯿ ﺪﻨﮐ ﺮ . ﺲـﭘ
ﺘﺳﺎﺸـﻧ دﺎﻤﺠﻧا زا لﻮـﮑﻟﻮﻣ ،ﻪ
ﺎـﻫ ي لژ رد بآ ﺎـﻫ
ي ﻪـﺑ ﻪﺘﺳﺎﺸـﻧ
ﺮﮐ ﯾ لﺎﺘﺴ ﺎﻫ ي ﯾ ﺪﺒﺗ ﺦ ﯾ ﻣ ﻞ ﯽ لﻮﮑﻟﻮﻣ ﻊﻤﺠﺗ ﻪﺑ ﺮﺠﻨﻣ و ﺪﻧﻮﺷ ﺎﻫ
ي
ﻣ ﻪﺘﺳﺎﺸﻧ ﯽ ﺪﻧﻮﺷ . لژ ،بوذ زا ﺲﭘ ﺎﻫ
ي ﺎﻫزﺎﻓ ﻪﺑ ﻪﺘﺳﺎﺸﻧ ي
ﻨﻏ ﯽ
ﺴﻘﺗ ﻪﺘﺳﺎﺸﻧ زا ﯿ
ﻣ ﻢ ﯽ ﺳ و ﺪﻧﻮﺷ ﯿ رﺮﻨ ﯾ جرﺎﺧ بآ و هدﺎﺘﻓا قﺎﻔﺗا ﺲ
ﻣ ﯽ ﺳآ ﻪﺑ ﺮﺠﻨﻣ ﻪﮐ ددﺮﮔ ﯿ
ﻣ ﻪﺘﺳﺎﺸﻧ ﺐ ﯽ
دﻮﺷ ] 5 .[
ﺛﺎﺗ ﯿ ﺮ -α ﻣآ ﯿ ز نﺎﻘﻘﺤﻣ ﻂﺳﻮﺗ ﻪﺘﺳﺎﺸﻧ رﺎﺘﺧﺎﺳ ﺮﺑ زﻼ ـﯾ
دﺎ ي درﻮـﻣ
ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﻪﻌﻟﺎﻄﻣ ]
9 - 6 .[
ﻪﻌﻟﺎﻄﻣ رد ا ي ﺪـﺑﺎﻋ ﻂـﺳﻮﺗ ي
و
نارﺎﮑﻤﻫ ) 2022 ( ﺛﺎﺗ ﯿ ﻣآ ﺎﻔﻟآ ﺮ ﯿ ﭘ ﻪﺘﺳﺎﺸﻧ ﺮﺑ زﻼ ﯿ
ﺗ ﺶ ﯿ ﺎﺑ هﺪﺷ رﺎﻤ
ﺳ ﯿ ﻞﮑ ﺎﻫ ي دﺎﻤﺠﻧا ماﺪﻣ -
ﺎﻣا ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ بوذ
ﺛﺎﺗ نﻮﻨﮐﺎﺗ ﯿ ﺮ -α ﻣآ ﯿ ﺮﺑزﻼ ﭘ ﻪﺘﺳﺎﺸﻧ ﯿ ﺗ ﺶ ﯿ ﺎﻤ رد دﺎـﻤﺠﻧا ﺎـﺑ هﺪﺷ ر
نﺎﻣز ﺎﻫ ي ﺳرﺮﺑ درﻮﻣ ﻒﻠﺘﺨﻣ ﯽ
دﺮﮕﻧ ﯾ ﺖﺳا هﺪ . ا رد ـﯾ ﻘﺤﺗ ﻦ ـﯿ ﻖ
ﺛﺎﺗ ﯿ نﺎﻣز ود ﺮ 4
و 8 ﺎـﻣد رد دﺎﻤﺠﻧا زور ي
C 25° - ناﻮـﻨﻋ ﻪـﺑ
ﭘﯿ ﺗ ﺶ ﯿ ـﺳرﺮﺑ ﻪﺘﺳﺎﺸـﻧ رﺎﺘﺧﺎـﺳ ﺮـﺑ رﺎـﻤ ﯽ
ﻠﺑﺎﻘﺴـﭙﺳ و ﺪـﺷ ﯿ
ﺖ
ﻫﯿ ﻟورﺪ ﯿ ﺰﻧآ ﺰ ﯾ ﻣآ ﻢ ﯿ ـﺳ ﻪﺘﺳﺎﺸﻧ ﺮﺑزﻼ ﯿ
ﻣز ﺐ ﯿ ـﻨ ﯽ ﺒﻃ ﯿ ـﻌ ﯽ ﻐﺗ و ﯿ ـﯿ ﺮ
ﯾ ﺳرﺮﺑ درﻮﻣ دﺎﻤﺠﻧا ﺎﺑ ﻪﺘﻓﺎ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
2 - شور و داﻮﻣ ﺎﻫ
ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﻣز ﺐ ﯿﻨ ﯽ وﺎﺣ ي 64 / 13 % ،ﺖﺑﻮﻃر 94 /0 % ﺑﺮﭼ ﯽ ،
26 / 11 % ﺌﺗوﺮﭘ ﯿ و ﻦ 78 / 18 % ﻣآ ﯿ زادﺮﭘ ﻪﺷﻮﺧ ﺖﮐﺮﺷ زا زﻮﻠ
ﺮﺧ سرﺎﻓ ﯾ راﺪ ي ﺪﺷ . ﮐﺮﺗ ﯿ شور سﺎﺳا ﺮﺑ تﺎﺒ ﺳ آ آ
ﯽ ﺳ ﯽ
) 2000 ( هزاﺪﻧا ﮔﯿ ﺮ ي ﺪﻧﺪﺷ .
[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
2 - 1 - ﺪﻤﺠﻨﻣ ﻪﺘﺳﺎﺸﻧ
ﻟﻮﺗ ﺖﻬﺟ ﯿ ﻟﻮﻣ شور زا ،ﺪﻤﺠﻨﻣ ﻪﺘﺳﺎﺸﻧ ﺪ ﯿ
و ﺎﻨ نارﺎﮑﻤﻫ ) 2016 (
ﺪﺷ هدﺎﻔﺘﺳا .
ﺴﻧﺎﭙﺳﻮﺳ ﯿ وﺎﺣ نﻮ ي ﻪﺘﺳﺎﺸﻧ ) 20 % (dwb بآ رد
ﻪﻧﻮﻤﻧ ﺲﭙﺳ و ﺪﺷ هداد راﺮﻗ ﺐﻟﺎﻗ رد ﺮﻄﻘﻣ تﺪﻣ ﻪﺑ ﺎﻫ
4 و 8
ﺎﻣد رد زور ي C 25° - ﺪﻧﺪﺷ ﺪﻤﺠﻨﻣ ]
10 .[
2 - 2 - ﻫ ﯿ ﻟورﺪ ﯿ ﺰﻧآ ﺰ ﯾﻤ ﯽ ﻪﺘﺳﺎﺸﻧ ﯾ ﻢ
ﺮﻓﺎﺑ زا هدﺎﻔﺘﺳا ﺎﺑ 50
ﻣ ﯿﻠ ﯽ ﺪﺳ تﺎﺘﺳا رﻻﻮﻣ ﯾ
ﻢ ) pH 5.5 ،
وﺎﺣ ي 02 /0 ﺪﺳ ﺪﺻرد ﯾ
ﻢ زآ ﯾﺪ ( ﺳ ﻪﺘﺳﺎﺸﻧ ، ﯿ
ﻣز ﺐ ﯿﻨ ﯽ ﺒﻃ ﯿﻌ ﯽ ،
رد هﺪﺷ دﺎﻤﺠﻧا ﻪﺘﺳﺎﺸﻧ 4
زور رد هﺪﺷ دﺎﻤﺠﻧا ﻪﺘﺳﺎﺸﻧ و (P4)
8 زور ﻞﮐ نزو ﺎﺗ (P8)
100 ﮐﺮﺘﻣﺮﮔ ﯿ ﺪﻧﺪﺷ ﺐ . ندوﺰﻓا زا ﺲﭘ
ﻣآ ﺎﻔﻟآ ﯿ ﺢﻄﺳ رد ﻪﺘﺳﺎﺸﻧ بﺎﻏود ﻪﺑزﻼ 15
/0 ) % ،w/w dbg
ﮏﺸﺧ ﻪﺘﺳﺎﺸﻧ (
ﺮﻄﺑ ، ي ﻘﻓا ترﻮﺻ ﻪﺑ ﻪﻧﻮﻤﻧ ﯽ
ﺎﺑ بآ مﺎﻤﺣ رد
ﺎﻣد ي C 45 °
و 150 ﻗد رد رود ﯿ
ا ؛ﺪﻧﺪﺷ هداد نﺎﮑﺗ ﻪﻘ ﯾ
ﻪﺑ ﻦ
ﺰﻧآ حﻼﺻا عوﺮﺷ رﻮﻈﻨﻣ ﯾﻤ
ﯽ ﺪﺷ مﺎﺠﻧا . ﻫﯿ ﻟورﺪ ﯿ ﺰﻧآ ﺰ ﯾﻤ ﯽ ﻪﺑ
تﺪﻣ 10 دﻮﺑ مﺎﺠﻧا لﺎﺣ رد ﺖﻋﺎﺳ .
ﺑ لﻮﻧﺎﺗا ﻢﺠﺣ رﺎﻬﭼ ﯽ
بآ
ﺎﻬﻧ ﻞﺣاﺮﻣ رد ﯾ
ﯽ ﮐﺮﺗ ﻪﺑ ﯿ ﺪﺷ ﻪﻓﺎﺿا ﺐ .
اﺮﺑ ،نآ زا ﺲﭘ ي
ﺎﭘ ﯾ نﺎ
ﺰﻧآ ﺶﻨﮐاو ﻪﺑ نداد ﯾﻤ
ﯽ ﺘﺳد ترﻮﺻ ﻪﺑ تﺪﺷ ﻪﺑ طﻮﻠﺨﻣ ، ﯽ
اد نﺎﮑﺗ ﺪﺷ هد . زا هدﺎﻔﺘﺳا ﺎﺑ 200
ﻣ ﯿﻠ ﯽ ﻟ ﯿ ﺑ لﻮﻧﺎﺗا ﺮﺘ ﯽ
،بآ
ﺪﺷ ﻪﺘﺴﺷ ﺎﻫرﺎﺑ و ﺎﻫرﺎﺑ ،دﻮﺑ هﺪﺷ حﻼﺻا ﻪﮐ ﻪﺘﺳﺎﺸﻧ .
ﻪﺑ ﺲﭙﺳ
تﺪﻣ 10 ﻗد ﯿ ﺮﻃ زا ﻪﻘ ﯾ ﺮﺘﻧﺎﺳ ﻖ ﯾﻔ ﯿ رود ﺎﺑ ژﻮ 5000
ﻗد ﺮﺑ ﯿ ﻪﻘ
زﺎﺑ ﯾﺑﺎ ﯽ ﺎﻣد رد و ﺪﺷ ي
C 40° ﺪﻧﺪﺷ ﮏﺸﺧ .
ﻫ ﺪﺻرد ﯿ ﻟورﺪ ﯿ ﺰ
ﻪﺘﺳﺎﺸﻧ ز ﻪﻟدﺎﻌﻣ زا ﺎﻫ ﯾ
ﺪﺷ ﺺﺨﺸﻣ ﺮ :
١٠٠ ﻫ ﻪﺘﺳﺎﺸﻧ نزو× ﯿ
ﻟورﺪ ﯿ هﺪﺷ ﺰ - ﻪﺘﺳﺎﺸﻧ نزو =
ﻫ ﺪﺻرد ﯿ ﻟورﺪ ﯿ ﺰ
ﻪﺘﺳﺎﺸﻧ نزو
2 - 3 - ﻣ ﯿ ﻧوﺮﺘﮑﻟا پﻮﮑﺳوﺮﮑ ﯽ
ﺸﺑور
2
ﯽ
ﻐﺗ ﻪﻌﻟﺎﻄﻣ ﯿﯿ
ﺤﻄﺳ تاﺮ ﯽ
لﻮﻧاﺮﮔ ﺎﻫ ي ﻪﺘﺳﺎﺸﻧ ﻂﺳﻮﺗ
ﻣﯿ ﻧوﺮﺘﮑﻟا پﻮﮑﺳوﺮﮑ ﯽ
ﺸﺑور ﯽ ﺪﺷ مﺎﺠﻧا . اﺮﺑ ي ا ﯾ رﻮﻈﻨﻣ ﻦ
ﮐﺪﻧا راﺪﻘﻣ ﯽ ﻪﻧﻮﻤﻧ زا ﻪﻧاد ﺎﺑ ﺎﻫ ﺪﻨﺑ ي ﯾ ﺧاﻮﻨﮑ ور ،ﺖ ي ﺎﭘ ﯾ ﺰﻠﻓ ﻪ ي
ﺶﺷﻮﭘ زا ﺲﭘ و هﺪﻧﺎﺒﺴﭼ ﻫد
ﯽ ﻪﻧﻮﻤﻧ ﻻ ﺎﺑ ﺎﻫ ﯾﻪ ا ي ﻪﺑ و ﻼﻃ زا
ﻪﻧﻮﻤﻧ ،نﻮﮔرآ زﺎﮔ رﻮﺒﻋ ﮏﻤﮐ ﻣ ﻂﺳﻮﺗ ﺎﻫ
ﯿ ﻧوﺮﺘﮑﻟا پﻮﮑﺳوﺮﮑ ﯽ
(Leica Cambridge, UK) ﺎﻤﻨﮔرﺰﺑ ﺎﺑ
ﯾ ﯽ دوﺪﺣ 1000
ا و ﺮﺑاﺮﺑ
ﯾ ﺴﻧﺎﺘﭘ فﻼﺘﺧا دﺎﺠ ﯿ
ﺮﺘﮑﻟا ﻞ ﯾﮑ ﯽ 20 kV ﻪﻌﻟﺎﻄﻣ درﻮﻣ
ﺖﻓﺮﮔ راﺮﻗ ]
2 .[
2 - 4 - ﻪﻌﺷا شاﺮﭘ ا ﯾ
ﺲﮑ
ا ﻪﻌﺷا ﺞﻨﺳ شاﺮﭘ زا هدﺎﻔﺘﺳا ﺎﺑ ﯾ
ﺲـﮑ ) لﺪـﻣ D8 Advance ،
نﺎﻤﻟآ ( ﻮﮕﻟا،
ي ا ﻮﺗﺮﭘ شاﺮﭘ ﯾ
ﻪـﻧﻮﻤﻧ ﺲﮑ ﺪـﺷ ﺺﺨﺸـﻣ ﺎـﻫ
] 3 .[
راﺰـﻓا مﺮـﻧ زا هدﺎﻔﺘـﺳا ﺎـﺑ ﻪﺘﺳﺎﺸﻧ رﻮﻠﺒﺗ ﻪﺟرد )
ﻪﺨﺴـﻧ ،EVA
9,0 ( ﺮﻃ زا ، ﯾ ﺴﻘﺗ ﻖ ﯿ ز ﺢﻄﺳ ﻢ ﯾ ﻪﻠﻗ ﺮ ـﻨﺤﻨﻣ ﺢﻄـﺳ ﺮـﺑ ﺎـﻫ ﯽ
ﻞـﮐ
ﻌﺗ ﯿﯿ ﺪﺷ ﻦ .
2 - 5 - ﻤﺧ صاﻮﺧ ﯿﺮ
ي نﺪﺷ
ﺳرﺮﺑ ﺖﻬﺟ ﯽ
ﺻﻮﺼﺧ ﯿﺎ ﻤﺧ ت ﯿﺮ ي ﻪﺘﺳﺎﺸﻧ نﺪﺷ هﺎﮕﺘـﺳد زا ﺎـﻫ
RVA(Newport Scientific Pty. Ltd, Warriewoof, Australia) ﺳا
ﺪﺷ هدﺎﻔﺘ . ﻤﻫ ﻪﺑ ﯿ زا رﻮﻈﻨﻣ ﻦ
ﻪﻧﻮﻤﻧ ﺮﻫ 5/
3 ﻪـﺑ و هﺪﺷ نزو مﺮﮔ 25
هدوﺰـﻓا ﺮـﻄﻘﻣ بآ مﺮـﮔ
ﺪﺷ . ﺘﺳﻼﭘ لاﺪﭘ ﯿ ﮑ ﯽ ﻣﻮﻟآ نﺰﺨﻣ دراو هﺎﮕﻨﺳد ﯿﻨﯿ
ﻣﻮ ﯽ وﺎـﺣ ي بآ
تﺪﻣ رد لاﺪﮔ ﺲﭙﺳ و هﺪﺷ ﻪﺘﺳﺎﺸﻧ و 10
ﻧﺎﺛ ﯿ لوا ﻪ 960 rpm
ﻪﻣادا رد و 160 rpm دﻮﺑ
. ﺳرﺮﺑ ﺖﻬﭼ ﯽ
ﺻﻮﺼﺧ ﯿ ﻤﺧ تﺎ ﯿﺮ ي
ﺒﻃ ﻪﺘﺳﺎﺸﻧ نﺪﺷ ﯿﻌ
ﯽ ﺗ و ﯿ تﺪﻣ ﻪﺑ اﺪﺘﺑا ،هﺪﺷ رﺎﻤ 2
ﻗد ﯿ ور ﻪـﻘ ي
°C 50 ﺪـﻧﺎﻣ ﺖﺑﺎﺛ .
ﺖﻋﺮـﺳ ﺎـﺑ ﺲﭙـﺳ 2/°C
0 ﻗد ﺮـﺑ ﯿ ﺎـﻣد ،ﻪـﻘ
ﻪﺑ
°C 90 و ﺪﺷ هﺪﻧﺎﺳر 3
ﻗد ﯿ ﻤﻫ رد ﻪﻘ ﯿ ﻗﺎﺑ ﺎﻣد ﻦ ﯽ ﺪﻧﺎﻣ . ﺲﭙﺳ
ور ﻪﺑ ﺎﻣد ﺖﻋﺮﺳ نﺎﻤﻫ ﺎﺑ ي
°C 50 ﺶﻫﺎﮐ ﯾ و ﺖﻓﺎ 5 ﻗد ﯿ رد ﻪـﻘ
اﯾ ﻗﺎﺑ ﺎﻣد ﻦ ﯽ ﺪﻧﺎﻣ ] 11 .[
2 - 6 - رﺎﻣآ حﺮﻃ ي
اﺮﺑ ي ﻟﺎﻧآ مﺎﺠﻧا ﯿ ﺎﻫﺰ ي هداد ﺳرﺮﺑ و ﺎﻫ ﯽ زا هﺪﻣآ ﺖﺳﺪﺑ تﺎﻋﻼﻃا
نﻮﻣزآ ﺎﻫ ي ﻓدﺎﺼﺗ ﻼﻣﺎﮐ حﺮﻃ زا ﻒﻠﺘﺨﻣ ﯽ
ﺪﺷ هدﺎﻔﺘﺳا .
ﻪﺑ
ﻌﺗ رﻮﻈﻨﻣ ﯿﯿ ﺑ فﻼﺘﺧا ﻦ ﯿ
ﻣ ﻦ ﯿ ﮕﻧﺎ ﯿ داﺪﻋا ﻦ ) راﺮﮑﺗ ﻪﺳ ﻞﻗاﺪﺣ
اﺮﺑ ي ﺎﻣزآ ﺮﻫ ﯾ ﺶ ( آ زا ﺲﭘ ، ﻟﺎﻧ
ﯿ راو ﺰ ﯾ
3ﺲﻧﺎ ﺪﻨﭼ نﻮﻣزآ زا
ﻪﻨﻣاد ا ي ﻦﮑﻧاد ﺢﻄﺳ رد 4
) 05 /0 P<
( دﺮﮔ هدﺎﻔﺘﺳا ﯾﺪ
. مﺎﻤﺗ رد
ﺰﺠﺗ ،ﻞﺣاﺮﻣ ﯾ
ﻠﺤﺗ و ﻪ ﯿ رﺎﻣآ ﻞ ي هداد راﺰﻓا مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ
SPSS 22 ﺖﻓﺮﮔ ترﻮﺻ
.
3. Analysis of variance
[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ
3 - 1 - ﺛﺎﺗ ﯿ ﻟﺎﻌﻓ ﺮ ﯿ ﺖ - α ﻣآ ﯿ ﺒﻃ ﻪﺘﺳﺎﺸﻧ ﺮﺑ زﻼ ﯿﻌ
ﯽ
ﭘ و ﯿ ﺗ ﺶ ﯿ دﺎﻤﺠﻧا ﺎﺑ رﺎﻤ
ﻞﮑﺷ رد ﻪﮐ رﻮﻄﻧﺎﻤﻫ 1
د ﯾ ﻣ هﺪ ﯽ لﻮﻧاﺮﮔ،دﻮﺷ ﺎﻫ
ي ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﺐ
ﻣز ﯿﻨ ﯽ ﻌﻠﺿ ﺪﻨﭼ و دﺮﮔ ﻞﮑﺷ ﯽ
ﺪﻧراد ] 12 .[
،دﺎﻤﺠﻧا لﺎﺒﻧد ﻪﺑ
لﻮﻧاﺮﮔ ﺢﻄﺳ ﺎﻫ
ي ﻓﺎﺻ ﻪﺘﺳﺎﺸﻧ ﯽ
ﻦﺸﺧ و هداد ﺖﺳد زا ار دﻮﺧ
ﻣ ﯽ ﺪﻧدﺮﮔ . ا ﺮﺑ هوﻼﻋ ﯾ
داﺪﻌﺗ ،دﺎﻤﺠﻧا زا ﺲﭘ،ﻦ ي
ﺮﮔ زا لﻮﻧا ﺎﻫ
وﺎﺣ ي شاﺮﺧ ،گرﺰﺑ ﺬﻓﺎﻨﻣ كﺮﺗ وﺎﻫ
ﺎﻫ ي ﻞﺑﺎﻗ گرﺰﺑ و ﮏﭼﻮﮐ
ور هﺪﻫﺎﺸﻣ ي
ز ﺪﻨﺘﺴﻫ ﺢﻄﺳ ﯾ
ﻟد ﻪﺑ دﺎﻤﺠﻧا ﺎﺑ اﺮ ﯿ
ﺑ لﺎﺼﺗا ﻞ ﯿ
ﻦ
كﺮﺗ و ﺬﻓﺎﻨﻣ ﺮﺘﮔرﺰﺑ ﺬﻓﺎﻨﻣ ،ﺎﻫ
ي ا لﻮﻧاﺮﮔ رد ﯾ
ﻣدﺎﺠ ﯽ ددﺮﮔ . ﻞﮑﺷ
2 ﻫ ﻪﺟرد ﯿ ﻟورﺪ ﯿ ﺳ ﻪﺘﺳﺎﺸﻧ ﺰ ﯿ
ﻣز ﺐ ﯿﻨ ﯽ ﺒﻃ ﯿﻌ ﯽ هﺪﺷ ﺪﻤﺠﻨﻣ و
و PF4 ﻣآ ﻂﺳﻮﺗ PF8
ﯿ ﻣ نﺎﺸﻧ ار زﻼ ﯽ
د ﺪﻫ . ﻫ زا ﻞﺒﻗ ﯿ ﻟورﺪ ﯿ ﺰ
ﺰﻧآ ﯾﻤ ﯽ اﺮﻓ ، ﯾ ﻟﻮﺗ ﺚﻋﺎﺑ دﺎﻤﺠﻧا ﺪﻨ ﯿ
ﺋﺰﺟ ﺪ ﯽ ﻫ ﻪﺘﺳﺎﺸﻧ ﯿ ﻟورﺪ ﯿ هﺪﺷ ﺰ
ﺳ رد ﯿ ﻣز ﺐ ﯿﻨ ﯽ ﺪﺷ ) :7PF4
%/
0 و 2PF8
%/
1 .(
ﺗ زا ﺲﭘ ﯿ رﺎﻤ
10 ﺎﺑ ﺖﻋﺎﺳ -α
ﻣآ ﯿ اﺰﻓا ،زﻼ ﯾ ﻫ ﻪﺟرد ﺶ ﯿ
ﻟورﺪ ﯿ ﺒﻃ ﻪﺘﺳﺎﺸﻧ ﺰ ﯿﻌ
ﯽ
هﺪﺷ ﺪﻤﺠﻨﻣ و و PF4
ﺗﺮﺗ ﻪﺑPF8 ﯿ ﻪﺑ ﺐ 8/
16
%،
1/
24 % و
8/
40 % اﺰﻓا ﯾ ﺶ ﯾ اﺮﺑﺎﻨﺑ ،ﺖﻓﺎ ﯾ
ﻦ ﻫ ﻪﺟرد ﺪﻧور ، ﯿ
ﻟورﺪ ﯿ زا ﺲﭘ ﺰ
ز ترﻮﺻ ﻪﺑ دﺎﻤﺠﻧا ﯾ
دﺮﮔ هﺪﻫﺎﺸﻣ ﺮ ﯾ
ﺪ : PF8E
<
PF4E
<
.PE
ﻫﯿ ﻟورﺪ ﯿ ﻣآ ﻂﺳﻮﺗ ﻪﺘﺳﺎﺸﻧ ﺰ ﯿ
دﺪﻌﺘﻣ ﻞﻣاﻮﻋ ﻪﺑ زﻼ ي
ﮕﺘﺴﺑ ﯽ دراد
ﮔ ﻊﺒﻨﻣ ،ﻪﺘﺳﺎﺸﻧ ﺖﻈﻠﻏ ﻞﻣﺎﺷ ﻪﮐ ﯿ
ﺳﺎﻨﺷ هﺎ ﯽ ،ﻪﺘﺳﺎﺸﻧ لﻮﻧاﺮﮔ
وﯾ ﮔﮋ ﯽ ﺎﻫ ي ﺢﻄﺳ ) لﺎﻧﺎﮐ و ﺬﻓﺎﻨﻣ دﻮﺟو ﺎﻫ
( ژﻮﻟﻮﻓرﻮﻣ ، ي لﻮﻧاﺮﮔ
) هزاﺪﻧا و ﻞﮑﺷ (
ﮐﺮﺗ ، ﯿ ﻟﻮﮑﻟﻮﻣ ﺐ ﯽ ) ﻣآ ﺖﺒﺴﻧ ﯿ ﻣآ و زﻮﻠ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻦ
ﺠﻧز لﻮﻃ و ﯿ
ﻣآ هﺮ ﯿ ﻣآ و زﻮﻠ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻦ ( ﻣ ﯽ ﺪﻨﺷﺎﺑ . ﻣآ ﯿ ﺎﻫزﻼ
لﻮﻧاﺮﮔ ﺎﻫ ي ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﻣز ﺐ ﯿﻨ ﯽ ﻫ درﻮﻣ شور ود ﺎﺑ ار ﯿ
ﻟورﺪ ﯿ ﺰ
ﻣ راﺮﻗ ﯽ ﺪﻫد : 1 - ﺰﻧآ ﯾ لﻮﻧاﺮﮔ ،ﻢ ﺮﻃ زا ار ﺎﻫ ﯾ
ﻖ
"
اﯾ رد هﺮﻔﺣ دﺎﺠ
5ﺢﻄﺳ ﻫﯿ ﻟورﺪ ﯿ ﻣ ﺰ ﯽ لﻮﻧاﺮﮔ و ﺪﻨﻨﮐ ﺎﻫ
ي ﺳ ﯿ ﻣز ﺐ ﯿﻨ ﯽ تﺎﻌﻄﻗ ﻪﺑ
ﺴﻘﺗ ﮏﭼﻮﮐ ﯿ
ﻣ ﻢ ﯽ ﺪﻧﻮﺷ . ﺘﻧ رد و ﯿ آﺮﻓ ﻪﺠ ﯾ ﻫ ﺪﻨ ﯿ ﻟورﺪ ﯿ ور ﺰ ي
،ﻪﺘﺳﺎﺸﻧ حﻮﻄﺳ -α
ﻣآ ﯿ ﺰﺠﺗ ﻪﺑ ردﺎﻗ زﻼ ﯾ
لﻮﮑﻟﻮﻣ ﻪ ﺎﻫ ي ﻣآ ﯿ رد زﻮﻠ
ﺑ ﻖﻃﺎﻨﻣ ﯽ ا ﻪﮐ ﺖﺳا ﻪﺘﺳﺎﺸﻧ ﻞﮑﺷ ﯾ
ﻞﺑﺎﻗ ﺶﻫﺎﮐ ﻪﺑ ﺮﺠﻨﻣ ﺮﻣﺎﻨ
ﻬﺟﻮﺗ ﯽ اﻮﺘﺤﻣ رد ي ﻣآ ﯿ ﺮﻫﺎﻇ زﻮﻠ ي ﻣ ﯽ دﻮﺷ . د رﺎﮐوزﺎﺳ ﯾ اﺮﺑ ﺮﮕ ي
5: Surface pitting
ﻫﯿ ﻟورﺪ ﯿ ﺳ ﺰ ﯿ ﻣز ﺐ ﯿﻨ ﯽ
، ا ﯾ ﻮﮕﻟا ﺎﺑ فﺎﮑﺷ دﺎﺠ ي
ﻫ ﯿ ﻟورﺪ ﯿ ﺰ
"
ﺎﺳﺮﻓ ﯾ ﺤﻄﺳ ﺶ
6ﯽ
"
ﺖﺳا .
ﻪﺘﺳﺎﺸﻧ ﺎﻫ ي ﻪﺘﺳﺎﺸﻧ ﻪﺑ ﺖﺒﺴﻧ ﺪﻤﺠﻨﻣ ﺎﻫ
ي ﺒﻃ ﯿﻌ ﯽ ﺳﺎﺴﺣ ﯿ ﺖ
ﺑﯿ ﺮﺘﺸ ي ﻫ ﻪﺑ ار ﯿ ﻟورﺪ ﯿ ﻂﺳﻮﺗ ﺰ -α
ﻣآ ﯿ زﻼ اﺰﻓا ﺎﺑ و ﺪﻧداد نﺎﺸﻧ ﯾ
ﺶ
زا دﺎﻤﺠﻧا نﺎﻣز 4
ﻪﺑ زور 8 ﻣ ،زور ﯿ ﺳﺎﺴﺣ ناﺰ ﯿ لﻮﻧاﺮﮔ ﺖ ﺎﻫ ي
ﺳآ ﯿ د ﺐ ﯾ ﺰﻧآ ﻪﺑ هﺪ ﯾ اﺰﻓا ﻢ ﯾ ﺶ ﯾ ﺖﻓﺎ ﻻد ﻪﮐ ﯾ دﺪﻌﺘﻣ ﻞ ﯾ اﺮﺒ ي نآ
دراد دﻮﺟو .
ﻣ ﻪﮐ ﺖﺳا هﺪﺷ شراﺰﮔ ﯿ
ناﺰ ﻫ ﯿ ﻟورﺪ ﯿ رﺎﺘﺧﺎﺳ ﺎﺑ ﺰ
ﺑ ﯽ لﻮﻧاﺮﮔ رد ﻢﻈﻧ ﺎﻫ
ي هﺪﺷ ﺪﻤﺠﻨﻣ )
هزاﺪﻧا ﮔﯿ ﺮ ي شاﺮﭘ ﻂﺳﻮﺗ
ا ﻪﻌﺷا ﯾ ﺲﮑ ( ﮕﺘﺴﺒﻤﻫ ﯽ دراد ﺖﺒﺜﻣ . اﺮﺑﺎﻨﺑ ﯾ ﻪﺘﺳﺎﺸﻧ رد ،ﻦ ﺎﻫ
ﯾ ﯽ ﺎﺑ
اﻮﺘﺤﻣ ﺖﺒﺴﻧ ي
ﺲﮑﻠﭙﻤﮐ ،ﻞﺨﻠﺨﺘﻣ رﺎﺘﺧﺎﺳ و ﺮﺗﻻﺎﺑ فرﻮﻣآ
ﺰﻧآ ﯾﻤ ﯽ - ﺮﺘﺴﺑﻮﺳ ﺮﺳ ا ﯾ ﻊ ﻣ قﺎﻔﺗا ﺮﺗ ﯽ
ﺪﺘﻓا . ﺪﻤﺠﻨﻣ ﻪﺘﺳﺎﺸﻧ رد
اراد ي ﻻﺎﺑ ﻪﺟرد ﯾ ﯽ ﺖﺴﻫ لﻮﻧاﺮﮔ ﺢﻄﺳ رد هﺮﻔﺣ و فﺎﮑﺷ زا
ﺰﻧآ دورو ﻪﮐ ﯾ
ﺮﺴﺗ لﻮﻧاﺮﮔ رﺎﺘﺧﺎﺳ ﻞﺧاد ﻪﺑ ار ﻢ ﯾ
ﻣ ﻊ ﯽ ﺪﻨﮐ .
ﺪﺑﺎﻋ ي ﺎﺘﻧ نارﺎﮑﻤﻫ و ﯾ
ﻬﺑﺎﺸﻣ ﺞ ﯽ ﺗ ﺖﺤﺗ ﻪﺘﺳﺎﺸﻧ ﻪﺑ ﻊﺟار ﯿ
رﺎﻤ
دﺎﻤﺠﻧا - ﺮﺨﺗ ﺎﺑ ﻪﮐ ﺪﻧدروآ ﺖﺳد ﻪﺑ بوذ ﯾ
ﻪﺘﺳﺎﺸﻧ رﺎﺘﺧﺎﺳ ﺐ
و ترذ ﺳ ﯿ ﻣز ﺐ ﯿﻨ ﯽ اﺮﻓ ﻂﺳﻮﺗ ﯾ
دﺎﻤﺠﻧا ﺪﻨ - رﺎﺘﺧﺎﺳ ،بوذ
اﺮﺑ ﺐﺳﺎﻨﻣ ﻪﺘﺳﺎﺸﻧ ي
ﺰﻧآ دورو ﯾ دﻮﺑ ﻢ ] 5 .[
ﻃ رد ﯽ آﺮﻓ ﯾ ﺪﻨ
،دﺎﻤﺠﻧا ﯾ ﻟﻮﺗ ﺦ ﯿ ﻠﺧاد ﺶﺨﺑ رد هﺪﺷ ﺪ ﯽ
ﯾ ﺟرﺎﺧ ﺎ ﯽ ،لﻮﻧاﺮﮔ
ﻬﺟﻮﺗ ﻞﺑﺎﻗ رﺎﺸﻓ ﯽ
) ﻧﯿ وﺮ ي ﻧﺎﮑﻣ ﯿ ﮑ ﯽ ( لﻮﻧاﺮﮔ ﻪﺑ ار ﺎﻫ
ي ﻪﺘﺳﺎﺸﻧ
ﻣ دراو ﯽ ﺪﻨﮐ . ﺘﻧ رد ﯿ لﻮﻧاﺮﮔ ،رﺎﺸﻓ ﻪﺠ ﺎﻫ
ي ﻪﺘﻓروﺮﻓ ﻪﺘﺳﺎﺸﻧ ﺮﺗ
ﻣ ﯽ ﺪﻧدﺮﮔ ] 13 و 14 .[
3 - 2 - ا ﻪﻌﺷا شاﺮﭘ ﯾ
ﺲﮑ
اﺮﺑ ي ﺳرﺮﺑ ﯽ ﺮﮐ رﺎﺘﺧﺎﺳ ﯾ
ﻟﺎﺘﺴ ﯽ ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﻣز ﺐ ﯿﻨ ﯽ ﺒﻃ ﯿﻌ ﯽ و
ﻮﮕﻟا زا هﺪﺷ ﺪﻤﺠﻨﻣ ي
ا ﻪﻌﺷا شاﺮﭘ ﯾ
ﺲﮑ ﺪﺷ هدﺎﻔﺘﺳا .
ﻞﮑﺷ رد
2 ﺳ ﻪﺘﺳﺎﺸﻧ شاﺮﭘ ﯿ
ﻣز ﺐ ﯿﻨ ﯽ ﺒﻃ ﯿﻌ ﯽ ﻧ رﺎﺘﺧﺎﺳ ، ﯿ
ﺮﮐ ﻪﻤ ﯾ ﻟﺎﺘﺴ ﯽ
عﻮﻧ ﭘ ﺎﺑ ار B ﯿ ﺎﻫ ﮏ ي رد ﺺﺧﺎﺷ 2θ
،ﻞﻣﺎﺷ ﻪﮐ 6/◦
5 ، 15◦ ،
17◦ ، 22◦ و 24◦ نﺎﺸﻧ ار ﻣ داد
ﯽ ﺪﻫد . هﺪﻫﺎﺸﻣ ﻪﮐ رﻮﻄﻧﺎﻤﻫ
ﻣ ﯽ ﺮﮐ ،دﻮﺷ ﯾ ﻟﺎﺘﺴ ﯿﺘ ﯽ زا 7/
37 ) % (NPS ﻪﺑ 8/
34 ) % (PF4 و
2/
33 ) % (PF8 ﺶﻫﺎﮐ ﯾ ﺖﻓﺎ . ﯾ هدﺮﺸﻓ ار لﻮﻧاﺮﮔ ،بآ ندز ﺦ
ﻣ ﯽ ارآ ﻪﺑ ﻪﮐ ﺪﻨﮐ ﯾ
ﺮﮐ ﺶ ﯾ ﻟﺎﺘﺴ ﯽ ﺳآ ﻪﺘﺳﺎﺸﻧ لﻮﻧاﺮﮔ ﻞﺧاد ﯿ
ﺐ
ﻣ ﯽ ﺑ ﺶﻨﮑﻤﻫﺮﺑ و ﺪﻧﺎﺳر ﯿ
ﭘرﺎﻣ ﻦ ﯿ ﭻ ﺎﻫ ي ﻀﺗ ار ﻪﻧﺎﮔود ﻌﯿ
ﻣ ﻒ ﯽ -
6: Surface pitting
[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
ﺪﻨﮐ . ﺑ ﻪﻘﻄﻨﻣ ءﺎﻘﺗرا ﯽ
ﺮﮐ ﺖﻟﺎﺣزا لﺎﻘﺘﻧا و ﻞﮑﺷ ﯾ
ﻟﺎﺘﺴ ﯽ ﺖﻟﺎﺣ ﻪﺑ
ﺮﺨﺗ ﺚﻋﺎﺑ ،دﺎﻤﺠﻧا زا ﺪﻌﺑ فرﻮﻣآ ﯾ
ﺑ ﺐ ﯿ ﺣﺎﻧ ﺮﺘﺸ ﯿ ﺮﮐ ﻪ ﯾ ﻟﺎﺘﺴ ﯽ و
ﻪﺟرد ﺶﻫﺎﮐ ﺮﮐ
ﯾ هﺪﺷ ﺪﻤﺠﻨﻣ ﻪﺘﺳﺎﺸﻧ نﺪﺷ ﻪﻟﺎﺘﺴ دﺮﮔ
ﯾ ا و ﺪ ﯾ ﻦ
اﺰﻓا ﺎﺑ ﺶﻫﺎﮐ ﯾ
دﺎﻤﺠﻧا نﺎﻣز ﺶ )
نﺎﻣز 8 ﻪﺑ ﺖﺒﺴﻧ زور 4
زور (
ﺖﺳا ﺮﺗدﻮﻬﺸﻣ .
ﻫ ﺎﺑ ﯿ ﻟورﺪ ﯿ ﺰﻧآ ﺰ ﯾ ﻢ ﺮﮐ ﻪﺟرد ، ﯾ
رد نﺪﺷ ﻪﻟﺎﺘﺴ
ﻪﺘﺳﺎﺸﻧ ﻪﺑ PE 2/
35 رد و و PF4E ﺗﺮﺗ ﻪﺑ PF8E
ﯿ ﻪﺑ ﺐ 4/
29
و 5/
25 ﭘ ﺶﻫﺎﮐ ﯿ دﺮﮐ اﺪ . ﭘﯿ ﺗ ﺶ ﯿ ﻞﺑﺎﻗ رﻮﻃ ﻪﺑ دﺎﻤﺠﻧا رﺎﻤ
ﻪﻈﺣﻼﻣ ا ي ﭘ ﯿ ﺎﻫﺪﻧﻮ ي ﻫ ﯿ ﻧژورﺪ ﯽ ﺑ و نورد ﯿ
ﻟﻮﮑﻟﻮﻣ ﻦ ﯽ ﺑ ﯿ ﻦ
لﻮﮑﻟﻮﻣ ﺎﻫ ي ﻣآ ﯿ ﻣآ و زﻮﻠ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻌﻀﺗ ار ﻦ ﯿ ﺘﻧ رد و دﺮﮐ ﻒ ﯿ
ﻪﺠ
ﺬﭘ فﺎﻄﻌﻧا ﯾﺮ ي ﻮﮑﻟﻮﻣ كﺮﺤﺗ و ﻣآ ل
ﯿ ﻣآ و زﻮﻠ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﺖﻬﺟ ار ﻦ
ﺬﭘ ﯾ ﺰﻧآ شﺮ ﯾ ﻫ ﻢ ﯿ ﻟورﺪ ﯿ اﺰﻓا هﺪﻨﻨﮐ ﺰ ﯾ
داد ﺶ ] 15 و 16 .[
اﯾ ﺘﻧ ﻦ ﯿ ﻪﺠ
ﻣ ار ﯽ ﺮﻃ زا ناﻮﺗ ﯾ
ا ﻖ ﯾ ﻌﻗاو ﻦ ﯿ ﺿﻮﺗ ﺖ ﯿ ﭘ ﻪﮐ داد ﺢ ﯿ ﺗ ﺶ ﯿ ﺎﻫرﺎﻤ ي ﺠﻧز ﻦﺘﻓﺎﮑﺷ ،دﺎﻤﺠﻧا
ﯿ هﺮ ﺎﻫ ي ﻣآ ﯿ ﻣآ و زﻮﻠ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻬﺴﺗ ار ﻦ ﯿ ﻞ
ﻣ ﯽ ز ﺪﻨﮐ ﯾ ا لﻮﺌﺴﻣ اﺮ ﯾ
ﺣﺎﻧ دﺎﺠ ﯿ ﺮﮐ ﻪ ﯾ ﻟﺎﺘﺴ ﯽ لﻮﻧاﺮﮔ رد ﺎﻫ
ي
ﺘﺳﺎﺸﻧ ﺪﻨﺘﺴﻫ ﻪ . اﺮﺑﺎﻨﺑ ﯾ ﺠﻧز ،ﻦ ﯿ هﺮ ﺎﻫ ي ﻟﻮﺗ هﺎﺗﻮﮐ ﺐﻌﺸﻨﻣ ﯿ
هﺪﺷ ﺪ
) ﻫ سﺎﺳا ﺮﺑ ﯿ
ﻟورﺪ ﯿ ﻪﺧﺎﺷ ﺰ ﺎﻫ ي ﻣآ هﺎﺗﻮﮐ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻣآ ﺎﺑ ﻦ ﯿ زﻮﻠ (
ﺎﻫ ﺶﻨﮐ ﻢﻫﺮﺑ ﺪﻨﺘﺴﻧاﻮﺘﻧ ي
ﺮﮐ دﺪﺠﻣ ﯾ ﻟﺎﺘﺴ ﯽ ا ﯾ رد و ﺪﻨﻨﮐ دﺎﺠ
ﺘﻧﯿ ﺮﮐ ﻪﺟرد ﻪﺠ ﯾ
ﻟﺎﺘﺴ ﯽ ﭘ ﺶﻫﺎﮐ ﯿ دﺮﮐ اﺪ ]
17 و 18 .[
ﻂﺳﻮﺗ هﺪﺷ مﺎﺠﻧا تﺎﻌﻟﺎﻄﻣ رد Benavent-Gil
و Rosell لﺎﺳ رد (2017a, 2017b) ﻪﮐ ﺪﺷ هداد نﺎﺸﻧ ،
ﻫﯿ ﻟورﺪ ﯿ ﻪﺘﺳﺎﺸﻧ ﺰ ﺎﻫ ي ﺒﻃ ﯿﻌ ﯽ ﺳ ،ﺞﻧﺮﺑ ،ترذ ﯿ
ﻣز ﺐ ﯿﻨ ﯽ مﺪﻨﮔ و
رﻮﻃ ﻪﺑ ﻬﺟﻮﺗ ﻞﺑﺎﻗ
ﯽ ﺠﻧز لﻮﻃ ﯿ
هﺮ ﺎﻫ ي ﻣآ ﯿ و زﻮﻠ
ﻨﭽﻤﻫ ﯿ ﻦ ﻣآ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻦ ﺶﻫﺎﮐ ار ﻣ
ﯽ ﺪﻫد ] 6 - 9 .[
NPS PE
PF4 PF4E
PF8 PF8E
Fig 1 Scanning electron micrographs of native, freezed and hydrolyzed potato starch.
[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
Fig. 2 X-ray diffraction patterns of native, freezed and hydrolyzed potato starch.
3 - 3 - ﺻﻮﺼﺧ ﯿ
ﻤﺧ تﺎ ﯿﺮ ي نﺪﺷ
ﻞﮑﺷ 3 ﺻﻮﺼﺧ ﯿ ﻤﺧ تﺎ ﯿﺮ ي ﺒﻃ ﻪﺘﺳﺎﺸﻧ نﺪﺷ ﯿﻌ
ﯽ ﺗ ﺖﺤﺗ ، ﯿ رﺎﻤ
ﻫ و دﺎﻤﺠﻧا ﯿ
ﻟورﺪ ﯿ ﻂﺳﻮﺗ ﺰ -α ﻣآ ﯿ ﻣ نﺎﺸﻧ ارزﻼ ﯽ
ﺪﻫد . ﻪﮐ رﻮﻄﻧﺎﻤﻫ
ﭘ ،ﺪﺷ هداد نﺎﺸﻧ ﯿ
ﺗ ﺶ ﯿ رد دﺎﻤﺠﻧا رﺎﻤ 4
و 8 ﺎﻣد رد زور ي
C 25 ° - ﻬﺟﻮﺗ ﻞﺑﺎﻗ رﻮﻃ ﻪﺑ ، ﯽ
ﺎﻣد ي ﻤﺧ ﯿﺮ ي نﺪﺷ ) (،PT
وﺮﺜﮐاﺪﺣ ﯾ زﻮﮑﺴ ﯾ ﻪﺘ ) (PV و و ﯾ زﻮﮑﺴ ﯾ ﺎﻬﻧ ﻪﺘ ﯾ ﯽ ) (FV ﺶﻫﺎﮐ ار
داد . لوﺪﺟ رد ﻪﮐ رﻮﻄﻧﺎﻤﻫ 3
ﺎﻣد ،ﺪﺷ هﺪﻫﺎﺸﻣ ي
ﻤﺧ ﯿﺮ ي
رد نﺪﺷ ) PF4
3/
0 7/± 63 ( و ) PF8 6/
0 3/± 61 (
ﺎﻘﻣ رد ﯾ ﻪﺴ ﺎﺑ ) NPS 2/
0 4/± 65 ( ﻞﺑﺎﻗ ترﻮﺻ ﻪﺑ
رادﺎﻨﻌﻣ ي (p˂ 0.05) دﻮﺑ ﺮﺘﻤﮐ
. ﺎﻫﺮﺘﻣارﺎﭘ ي ﻤﺧ ﺎﺑ ﻂﺒﺗﺮﻣ ﯿﺮ
ي
ﻣ نﺪﺷ ﯽ ﺛﺄﺗ ﺖﺤﺗ ﺪﻨﻧاﻮﺗ ﯿ
دﺪﻌﺘﻣ ﻞﻣاﻮﻋ ﺮ ي
ﺶﺨﺑ ﺖﺒﺴﻧ ﺪﻨﻧﺎﻣ
ﺮﮐ ﻪﺑ فرﻮﻣآ ﯾ
ﺠﻧز لﻮﻃ ،لﺎﺘﺴ ﯿ
ﻣآ هﺮ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻣآ ﺖﺸﻧ ،ﻦ ﯿ
،زﻮﻠ
ﺑ كﺎﮑﻄﺻا ﯿ
لﻮﻧاﺮﮔ ﻦ ﺎﻫ ي لﻮﻧاﺮﮔ مرﻮﺗ و ،مرﻮﺘﻣ دﺮﮐ هرﺎﺷا ﺎﻫ
.
لﻮﻧاﺮﮕﻨﺘﺴﮑﺷ ﺎﻫ
ي ﭘ ﻦﺘﺴﮑﺷ و ﻪﺘﺳﺎﺸﻧ ﯿ
ﺎﻫﺪﻧﻮ ي ﺑو ﻞﺧاد ﯿ ﻦ
ﻣ ﻟﻮﮑﻟﻮ ﯽ ﻫ ﯿ ﺠﻧز رد نژورﺪ ﯿ
هﺮ ﺎﻫ ي ﺗ رد ﻪﺘﺳﺎﺸﻧ ﯿ
دﺎﻤﺠﻧارﺎﻤ
) ﻫ ﺪﺻرد ﻪﺑ طﻮﺑﺮﻣ ﺶﺨﺑ ﯿ
ﻟورﺪ ﯿ و ﺰ X-ray (،
دازآ ﻪﺑ ي ا ﯾ ﻦ
ﺠﻧز ﯿ هﺮ ﻣ ﮏﻤﮐ ﺎﻫ ﯽ
ﺪﻨﮐ . ﺘﻧ رد ﯿ لﻮﮑﻟﻮﻣ ،ﻪﺠ ﺎﻫ
ي ﺑ بآ ﯿ ﻪﺑ ﺮﺘﺸ
لﻮﻧاﺮﮔ نورد ﺎﻫ
ﯾﭙﯿ ﺗ ﺶ ﯿ ﻣ ذﻮﻔﻧ دﺎﻤﺠﻧا ﺎﺑ هﺪﺷ رﺎﻤ ﯽ
و ﺪﻨﻨﮐ
ﻣآ ﯿ ﻣ زﻮﻠ ﯽ ﻪﻧاد زا ﺪﻧاﻮﺗ ﺗﻻژ و دﻮﺷ جرﺎﺧ ﺎﻫ
ﯿﻨ ﺎﺑ ار نﺪﺷ ﻪ
ﺎﻣد ﯾ ﻤﺨ ﯿﺮ ي ﺮﺴﺗ ﺮﺘﻤﮐ نﺪﺷ ﯾﻊ
ﺪﻨﮐ . ﺘﻧ رد ﯿ ا ﻪﺠ ﯾ ،لﻼﺘﺧا ﻦ ﯾ
ﮏ زﺎﺑ رﺎﺘﺧﺎﺳ
) ﺶﺨﺑ X-ray و (SEM ﮑﺸﺗ ﯿ ﺑ ﻞﻣﺎﻌﺗ و ﺪﺷ ﻞ ﯿ
ﻦ
لﻮﮑﻟﻮﻣ ﺎﻫ ي ﺮﻃ زا ار بآ و ﻪﺘﺳﺎﺸﻧ ﯾ
هوﺮﮔ ﻖ ﺎﻫ ي ﻬﺴﺗ OH ﯿ ﻞ
دﺮﮐ . ﭽﻤﻫ ﯿﻨﯿ ﺎﻣد توﺎﻔﺗ ،ﻦ ي
ﻤﺧ ﯿﺮ ي ﺑ نﺪﺷ ﯿ ﻪﺘﺳﺎﺸﻧ ﻦ ﺎﻫ ي
ﺒﻃ ﯿﻌ ﯽ ﻫ ﯿ ﻟورﺪ ﯿ ﻪﺘﺳﺎﺸﻧ و هﺪﺷ ﺰ ﺎﻫ
ي ﭘ ﯿ ﺗ ﺶ ﯿ رﺎﻤ دﺎﻤﺠﻧا ﺎﺑ هﺪﺷ
ﻫﯿ ﻟورﺪ ﯿ و هﺪﻨﻫد نﺎﺸﻧ ،هﺪﺷ ﺰ ﯾ
ﮔﮋ ﯽ ﺎﻫ ي ) ﺠﻧز لﻮﻃ ﯿ نزو و هﺮ
ﻟﻮﮑﻠﻣ ﯽ ( ﻣآ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﺖﺳا ﻦ . ﻟﻮﮑﻠﻣ نزو ﺶﻫﺎﮐ ﺎﺑ ﯽ
ﻣآ ﯿ ﺘﮑﭘﻮﻠ ﯿ ،ﻦ
ﺎﻣد ﺶﻫﺎﮐ ي ﻤﺧ ﯿﺮ ي ﺑ نﺪﺷ ﯿ ﺖﺳا دﻮﻬﺸﻣ ﺮﺘﺸ .
دﯾ ﻤﺧ صاﻮﺧ ﺮﮕ ﯿﺮ
ي و ﺮﺜﮐاﺪﺣ ﻞﻣﺎﺷ نﺪﺷ ﯾ
زﻮﮑﺴ ﯾ ﻪﺘ ) (PV و
وﯾ زﻮﮑﺴ ﯾ ﺎﻬﻧ ﻪﺘ ﯾ ﯽ ) (FV رد ،NPS ،PF4 لﺎﮑﺷا وPF8
ﻫﯿ رﺪ ﻟو ﯿ نآ هﺪﺷ ﺰ ا ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ رﻮﻃ ﻪﺑ ﺎﻫ
ي ﺶﻫﺎﮐ
ﯾ ﺖﻓﺎ ) لوﺪﺟ 1 .(
و ﺮﺜﮐاﺪﺣ ﯾ زﻮﮑﺴ ﯾ زا ﻪﺘ cP (NPS) 3678
ﻪﺑ
3206cP رد و PF4 3006cP رد ﺶﻫﺎﮐPF8 ﯾ ﺖﻓﺎ . ﺗ ﺎﺑ ﯿ رﺎﻤ
ﻣآ ﺎﻔﻟآ ﻪﺑ ﺎﻫ ﻪﺘﺳﺎﺸﻧ عاﻮﻧا ﯿ
و ﺮﺜﮐاﺪﺣ ،زﻼ ﯾ
زﻮﮑﺴ ﯾ ﻪﺘﺳﺎﺸﻧ رد ﻪﺘ
ﻫﯿ ﻟورﺪ ﯿ ﺒﻃ هﺪﺷ ﺰ ﯿﻌ ﯽ ، و PF4 ﺗﺮﺗ ﻪﺑ PF8
ﯿ ﻪﺑ ﺐ 3113cP
،
2155cP و 1956cP ﭘ ﺶﻫﺎﮐ ﯿ دﺮﮐ اﺪ . دﺎﻘﻣ ﯾ ﺎﭘ ﺮ ﯾﯿ ﻦ ﺮﺗ PV
ﻪﺘﺳﺎﺸﻧ ﺎﻫ ﯾﭙﯿ ﺗ ﺶ ﯿ ﺎﻘﻣ رد دﺎﻤﺠﻧا ﺎﺑ هﺪﺷ رﺎﻤ ﯾ
ﺒﻃ ﻪﺘﺳﺎﺸﻧ ﺎﺑ ﻪﺴ ﯿﻌ
ﯽ
ﻫﯿ ﻟورﺪ ﯿ ﻣآ ﺎﻔﻟآ ﻂﺳﻮﺗ هﺪﺷ ﺰ ﯿ
ﻣ نﺎﺸﻧ ﺎﻫزﻼ ﯽ
ﭘ ﻪﮐ ﺪﻫد ﯿ ﺶ
ﺗﯿ ﻻد ﻪﺑ دﺎﻤﺠﻧارﺎﻤ ﯾ
لﻮﻧاﺮﮔ ﻪﺑ تﺪﺷ ﻪﺑ ﻒﻠﺘﺨﻣ ﻞ ﺎﻫ
ي ﻪﺘﺳﺎﺸﻧ
ﺳآ ﯿ ﻣ ﺐ ﯽ و و ﺪﻧﺎﺳر ﯾ
زﻮﮑﺴ ﯾ ﻣ ﺶﻫﺎﮐ ار ﻪﺘ ﯽ
ﺪﻫد : 1 - رﺎﺘﺧﺎﺳ
ﭘ ﻦﺘﺴﮑﺷ ﺎﺑ ﻪﺘﺳﺎﺸﻧ ﯿ
ﺎﻫﺪﻧﻮ ي ﻫ ﯿ ﻧژورﺪ ﯽ ﺮﮐ رﺎﺘﺧﺎﺳ و ﯾ
ﻟﺎﺘﺴ ﯿ زﺎ
Crystallinity (%): 25.5± 0.3e Hydrolysis (%): 40.8± 0.6a Crystallinity (%): 29.4± 0.2d Hydrolysis (%): 24.1± 0.4b Crystallinity (%): 34.3± 0.3b Hydrolysis (%):16.8± 0.5c Crystallinity (%): 33.2± 0.5c Hydrolysis (%): 1.2± 0.3d
Crystallinity (%): 34.8± 0.4b Hydrolysis (%): 0.7± 0.1e Crystallinity (%): 37.7± 0.3a Hydrolysis (%): 0f
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ﻣ ﻪﺘﺴﺴﮔ ﻢﻫ ﯽ
دﻮﺷ . 2 - خارﻮﺳ و ﺎﻫ كﺮﺗ ﺎﻫ
ي ﺑ ﯿ ﺢﻄﺳ رد ﺮﺘﺸ
ﮔرﺎﭘ ﻪﺑ ﺮﺠﻨﻣ،لﻮﻧاﺮﮔ ﯽ
ﮑﻔﺗ و ﻪﺘﺳﺎﺸﻧ ﯿ
لﻮﮑﻟﻮﻣ ﮏ ﺎﻫ
ي
ﻣآ ﯿ ﺘﮑﭘﻮﻠ ﯿ ﻣآ و ﻦ ﯿ زﻮﻠ دﺮﮔ ﯾ هﺪ ﮑﺸﺗ و ﯿ ﺮﺘﺴﮐد ﻞ ﯾ ﻦ ﻮﻘﺗ ار ﯾ ﺖ
ﺸﺨﺑ ﯿ ﺗﻻژ رد ﻪﮐ هﺪ ﯿ
ﺮﺘﻤﮐ مرﻮﺗ نﺪﺷ ﻪﻨ ي
ﺎﺸﻧ ﻣ ن ﯽ ﺪﻫد . اﯾ ﻦ ﺎﺘﻧ
ﯾ ﺎﺘﺳار رﺪﺠ ي
ﺎﺘﻧ ﯾ ﻟ زا ﻞﺻﺎﺣ ﺞ ﯽ
نارﺎﮑﻤﻫ و )
2021 ( ،
نارﺎﮑﻤﻫ و ﮓﻧﻮﺳ )
2020 ( و ﮓﻧاژ و
نارﺎﮑﻤﻫ )
2021 ( ﻣ ﯽ ﺪﺷﺎﺑ ] 17 ، 19 ، 20 .[
Fig 3 RVA of native, freeze-thawed and hydrolyzed potato and corn starch.
Table 1 Pasting profile and thermal properties of native, freezed and hydrolyzed potato starch Pasting
temperature (°C)
Peak viscosity (mPa•s)
Final viscosity
(mPa•s) P 65.4± 0.2B 3678± 23A 1762± 15A
PE* 65.8± 0.1A 3113±13C 1267±17C PF4 63.7± 0.3C 3206±15B 1367± 8B
PF4E 57.7± 0.4E 2155± 15E 1008±11E
PF8 61.3± 0.6D 3006± 23D 1221± 12D
PF8E 52.6± 0.7F 1956±17F 971± 12F
** Different capital letters are significantly different (p <0.05).
4 - ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي ﻠﮐ ﯽ
ا رد ﯾ ﻣآ ﺮﺛا ،ﻪﻟﺎﻘﻣ ﻦ ﯿ
و ﺮﺑ زﻼ ﯾ ﮔﮋ ﯽ ﺎﻫ ي رﺎﺘﺧﺎـﺳ ي دﺮـﮑﻠﻤﻋ و ي
ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﺐ ﻣز ﯿﻨ ﯽ ﺒﻃ ﯿﻌ ﯽ ﺗ و ﯿ نﺎﻣز رد دﺎﻤﺠﻧا ﺎﺑ هﺪﺷ رﺎﻤ ﺎـﻫ
ي
ﺳرﺮﺑ ﻒﻠﺘﺨﻣ ﯽ
ﺪﺷ . اﺮﻓ ﯾ ﻧا ﺪﻨ ﻬﺟﻮـﺗ ﻞﺑﺎﻗ رﻮﻃ ﻪﺑ دﺎﻤﺠ ﯽ
ﺢﻄـﺳ
وﯾ اﺰﻓا ار ﺬﻓﺎﻨﻣ هزاﺪﻧا و هﮋ ﯾ
اﺰﻓا ﺚﻋﺎﺑ و هداد ﺶ ﯾ
ﺳﺎﺴـﺣ ﺶ ﯿ
ﺖ
ﻠﺑﺎﻗ ﺮﺑاﺮﺑ رد ﻪﺘﺳﺎﺸﻧ ﯿ
ﺰﻧآ ﻢﻀﻫ ﺖ ﯾﻤ
ﯽ ﻣ ﯽ دﻮﺷ . ﻫﯿ ﻟورﺪ ﯿ ﻪﺘﺳﺎﺸﻧ ﺰ
ﺒﻃ ﯿﻌ ﯽ ﺗ ﯿ ﺑ ﻪـﺑ ﺮﺠﻨﻣ ،دﺎﻤﺠﻧا ﺎﺑ هﺪﺷ رﺎﻤ ـﯿ
ﺮﺘﺸ ﯾ ـﺑ ﻦ ﯽ ـﻤﻈﻧ ﯽ رد
ﺮﮐ رﺎﺘﺧﺎﺳ ﯾ ﻟﺎﺘﺴ ﯽ ) ﺶﻫﺎﮐ ( ژﻮﻟﻮﻓرﻮﻣ ، ي
) اﯾ كﺮـﺗ و ﺬﻓﺎﻨﻣ دﺎﺠ (
،
ﺎﻣد ي ﺧ ﻤﯿ ﺮ ي و ﺮﺜﮐاﺪﺣ و نﺪﺷ ﯾ
زﻮﮑﺴ ﯾ ﻪﺘ ) ﺶﻫﺎـﮐ ( ـﻣ ﯽ دﻮـﺷ .
ﺗﯿ رﺎﻤ ﻪﺘﺳﺎﺸﻧ ﺰﻧآ ﺎﺑ ﯾ لﻮﻧاﺮﮔ رد ﻢ ﺎﻫ
ﯾ ﯽ اراد ﻪـﮐ ي فرﻮـﻣآ داﻮـﻣ
ﺑﯿ ﺮﺘﺸ ) اﻮﺘﺤﻣ ي ﺮﺘﻤﮐ ﺮﮐ ﯾ ﻟﺎﺘﺴ ﯿﻨﯿ ﺘ ﯽ ( دﻮـﺑ ﺮـﺗ ﻪـﺟﻮﺗ ﻞﺑﺎﻗ ،ﺪﻧدﻮﺑ .
ﺑﯿ ﺮﺘﺸ ﯾ ﺳﺎﺴﺣ ﻦ ﯿ ﻫ ﺮﺑاﺮﺑ رد ﺖ ـﯿ
ﻟورﺪ ﯿ زا ﺰ ـﻣآ-α ﯿ ﻪـﺑ ﻖـﻠﻌﺘﻣ زﻼ
ﺳ ﻪﺘﺳﺎﺸﻧ ﯿ ﻣز ﺐ ﯿﻨ ﯽ ﺗ ﯿ ﻣ ﻪﮐ دﻮﺑ دﺎﻤﺠﻧا ﺎﺑ هﺪﺷ رﺎﻤ ﯽ
ﺮـﺠﻨﻣ ﺪﻧاﻮﺗ
ﺰﻧآ فﺮﺼﻣ ﺶﻫﺎﮐ ﻪﺑ ﯾ
ﺎﻨـﺻ رد ﻢ ﯾ اﺬـﻏ ﻊ ﯾ ﯽ ﻂﺒﺗﺮـﻣ و داﻮـﻣ ﺎـﺑ
اﺬﻏ ﯾ ﯽ دﻮﺷ .
5 - ﻊﺑﺎﻨﻣ
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Jahromi, M. Niakousari, L. Torri, The effect
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[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
The effect of freezing time on the efficiency yield of potato starch hydrolysis by alpha-amylase
Abedi, E. 1*, Pourmohammadi, K. 1
1. AssociateProfessor of Food Science and Technology, School of Agriculture, Fasa University, Fasa, Iran.
ABSTRACT ARTICLE INFO
Article History:
Received 2022/ 07/ 07 Accepted 2022/ 08/ 09
Keywords:
Potato starch,
Freezing pretreatment, Crystallinity percentage, Morphological properties.
In order to achieve high-efficiency hydrolysis by α-amylase, freezing pretreatment of potato starch was performed at -25°C for 4 days (PF4) and 8 days (PF8). For this purpose, potato starch (NPS) and freeze pretreatment were suspended in water by exposure to alpha-amylase (0.15% w/v) for 10 hours at 25°C. Morphological properties, percentage of hydrolysis, degree of crystallinity by X-ray diffraction (X-ray) and pasting properties by rapid visco analyzer (RVA) were studied in native,freezed and hydrolyzed starches.Freezing caused surface damage of starch granule and according to the morphology results, scratches, dents and cracks were observed on the surface of the granule, and these changes were much more obvious after 8 days of freezing than after 4 days of freezing, and they were easily hydrolyzed. It was placed by α- amylase and caused the disruption of starch granules. The results of the hydrolysis percentage showed that freezing produced a trace percentage in the production of hydrolyzed starch. Hydrolysis percentage (%) of natural starch increased to 16.8%, 24.1% and 40.8% after hydrolysis in P, PF4 and PF8, respectively. In addition, the relative crystallinity after freezing decreased from natural starch (37.7%) to 34.8% in PF4 and 33.2% in PF8, and this decrease was very substantial(p˂ 0.05) after treatment with α-amylase which decreased to 34.3%, 29.4% and 25.5%
in P, 4 days and 8 days of freezing, respectively. Gelatinization temperature and pick viscosity after amylase treatment in all natural starches under freezing treatment decreased significantly (p˂ 0.05) and the decreasing trend was observed in the order of PF8 > PF4 > P, which granular and intramolecular structures disruption as well as low swelling power could be the reason behind these results.
DOI: 10.22034/FSCT.19.128.183
DOR: 20.1001.1.20088787.1401.19.128.27.2
*Corresponding Author E-Mail:
Journal of Food Science and Technology (Iran)
Homepage:www.fsct.modares.ir Scientific Research
[ DOR: 20.1001.1.20088787.1401.19.128.27.2 ] [ Downloaded from fsct.modares.ac.ir on 2023-10-02 ]
