ﺳرﺮﺑ ﯽ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ
ﮔﺪﻨﺸﮐ و ﯽ
چرﺎﻗ ﺎﻫ ي ﮏﭙﮐ ﻞﻣﺎﻋ ﮔدز
ﯽ ﻣ ﯿ لﺎﻘﺗﺮﭘ هﻮ
ﻨﭘ ) ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ( ﻫ هرﺎﺼﻋ زا هدﺎﻔﺘﺳا ﺎﺑ ﯿ
ﮑﻟاورﺪ ﯽ ﮔ ﯿ نﺎﻨﺷا هﺎ
ﻔﻄﺼﻣ ﯽ ﺘﻤﺣر ﯽ ﻨﺟ ﯿ دﺎﺑآﺪ
1
ﻠﻋ زوﺮﻬﺑ ،
*ﯿ ﻧﺎﻬﺒﻬﺑ هداﺰ
2
ﯽ دﺎﺷﻮﻧ ﺪﻤﺤﻣ ،
31 - دﺎﺘﺳا ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﻧﺎﺒﻏﺎﺑ ﯽ زروﺎﺸﮐ هﺪﮑﺸﻧاد ، ي
زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ
ﯽ ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ
ا ، ﯾ ناﺮ .
2 - دﺎﺘﺳا ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ، ﯽ
ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ
ﯽ
ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ
ا ، ﯾ ناﺮ .
3 - ﺸﻧاد ﯿ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ، ﯽ
ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ
ﯽ
ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ
ا ، ﯾ ناﺮ .
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 18 / 11 / 1400
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 15 / 01 / 1400
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
ﮔﯿ نﺎﻨﺷا هﺎ
،
ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ
،
ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
،
ﺘﻧآ ﺮﺛا ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ .
DOI: 10.52547/fsct.19.124.127
DOR: 20.1001.1.20088787.1401.19.124.30.7
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ : [email protected]
ﻫ هرﺎﺼﻋ ،ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ نﺎﻨﺷا هﺎ ) Seidlitziarosmarinus (
زا هدﺎﻔﺘﺳا ﺎﺑ
ﺧ شور ﯿ دﺮﮔ جاﺮﺨﺘﺳا نﺪﻧﺎﺴ ﯾ
ﻣﺪﺿ ﺮﺛا و ﺪ ﯿ
ﺑوﺮﮑ ﯽ ﻠﻋ نآ ﯿ چرﺎﻗ ﻪ ﺎﻫ ي ﮏﭙﮐ ﻞﻣﺎﻋ ﮔدز
ﯽ ﻣ ﯿ هﻮ
لﺎﻘﺗﺮﭘ ﻨﭘ) ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ( نﻮﻣزآ ﻖﺑﺎﻄﻣ ﺎﻫ
ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ ،رﺎﮔآ نژﻮ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ﯽ
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﯽ
ﺳرﺮﺑ درﻮﻣ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
ا ﺮﺑ هوﻼﻋ ﯾ
ﻣ ،ﻦ ﯿ ﺋﻮﻧوﻼﻓ و لﻮﻨﻓ ناﺰ ﯿ
ﻟﺎﻌﻓ و مﺎﺗ ﺪ ﯿ
آ ﺖ ﺘﻧ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻫ هرﺎﺼﻋ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ هﺎ
ﻌﺗ نﺎﻨﺷا ﯿﯿ دﺮﮔ ﻦ ﯾ ﺪ . ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ نﻮﻣزآ ﺞ ﺎﻫ ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ ﻟﺎﻌﻓ ،رﺎﮔآ ﮏﻫﺎﭼ و نژﻮ ﯿ
ﺪﺿ ﺖ
ﭼرﺎﻗ ﯽ و دﻮﺑ ﺖﻈﻠﻏ ﻪﺑ ﻪﺘﺴﺑاو هرﺎﺼﻋ ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ سﺎﺴﺣ ﺮﺗ ﯾ ﻮﺳ ﻦ ﯾ ﭼرﺎﻗ ﻪ ﯽ رد
دﻮﺑ هرﺎﺼﻋ ﺮﺑاﺮﺑ .
ﻮﺳ ود ﺮﻫ ،هوﻼﻌﺑ ﯾ
ﭼرﺎﻗ ﻪ ﯽ رﻮﻀﺣ رد 32
ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ردﺎﻗ هرﺎﺼﻋ ﺮﺘ
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﺪﻧدﻮﺒﻧ ﺪﺷر ﻪﺑ ﯽ
اﺮﺑ ﻨﭘي ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﺎﺑ ﺮﺑاﺮﺑ 512 ﻣ ﯿﻠ ﯽ رد مﺮﮔ
ﻣﯿ ﻠ ﯽ ﻟﯿ دﻮﺑ ﺮﺘ . اﻮﺘﺤﻣ ي ﺋﻮﻧوﻼﻓ و مﺎﺗ لﻮﻨﻓ ﯿ
ﺗﺮﺗ ﻪﺑ هرﺎﺼﻋ مﺎﺗ ﺪ ﯿ
ﺎﺑ ﺮﺑاﺮﺑ ﺐ mg GAE/g
19 / 26
و mg QE/g 10
/ 11 دﻮﺑ . ﺎﺘﻧ ﯾ ﻟﺎﻌﻓ ﺞ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻫ هرﺎﺼﻋ داد نﺎﺸﻧ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ هﺎ
دار رﺎﻬﻣ ﻪﺑ ردﺎﻗ نﺎﻨﺷا ﯾ
لﺎﮑ ﺎﻫ ي دازآ ) DPPH 14 / 112 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ ( و
)ABTS 46 / 103 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ ( ﻣ ﯽ ﺪﺷﺎﺑ . ﻠﮐ رﻮﻄﺑ ﯽ ﺎﺘﻧ ، ﯾ ا ﺞ ﯾ ﻣ نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﻦ ﯽ
ﺪﻫد
ﻫ هرﺎﺼﻋ ﻪﮐ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ وﺎﺣ نﺎﻨﺷا هﺎ ي
ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻠﺑﺎﻗ ﺎﺑ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
و
ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺑ ﻣ هﻮﻘﻟﺎ ﯽ ﺪﺷﺎﺑ .
ﻪﻠﺠﻣ
مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ
ناﺮ
www.fsct.modares.ac.ir
: ﻪﻠﺠﻣ ﺖ ﯾ ﺎﺳ
ﻪﻟﺎﻘﻣ
ﻤﻠﻋ
ﯽ
ﺸﻫوﮋﭘ
_ﯽ
1 - ﻪﻣﺪﻘﻣ
ﺑ رد و ﺪﻨﺘﺴﻫ هﺪﺷ ﻪﺘﺧﺎﻨﺷ نﺎﻬﺟ ﺮﺳاﺮﺳ رد تﺎﺒﮐﺮﻣ ﯿ
زا ﺶ 137
ﻟﻮﺗ رﻮﺸﮐ ﯿ رﺎﺠﺗ ﺪ ي ﺪﻧراد . ﻟﻮﺗ ﺢﻄﺳ ﻞﮐ ﯿ
تﺎﺒﮐﺮﻣ ﺪ )
ﻞﻣﺎﺷ
ﺮﮔ ،لﺎﻘﺗﺮﭘ ﯾ
ﭗ ﻟ ،توﺮﻓ ﯿ ﻟ ،ﻮﻤ ﯿ ﮕﻧرﺎﻧ ،شﺮﺗﻮﻤ ﯽ
ﻏ و ﯿ هﺮ ( نﺎﻬﺟ رد
دوﺪﺣ 7/
9 ﻣ ﯿﻠ ﯿ لﺎﺳ رد ﻪﮐ ﺖﺳا هدﻮﺑ رﺎﺘﮑﻫ نﻮ 2018
دوﺪﺣ
5/
138 ﻣ ﯿﻠ ﯿ ﻣ ﻦﺗ نﻮ ﯿ ﺖﺳا هﺪﺷ ﺖﺷادﺮﺑ نآ زا هﻮ ]
1 , 2 [ . ﻢﻬﺳ
ﻧﺎﻬﺟ دﺎﺼﺘﻗا رد تﺎﺒﮐﺮﻣ ﺖﻌﻨﺻ ﯽ
ﺴﺑ ﯿ ز رﺎ ﯾ اﺮﺑ و ﺖﺳا دﺎ ي
ﻣﯿ ﻠﯿ نﻮ ﻠﻤﻋ رد نﺎﻬﺟ ﺮﺳاﺮﺳ رد ﺮﻔﻧ ﺎﻫ ﯿ
ﺎﺠﺑﺎﺟ ،ﺖﺷادﺮﺑ تﺎ ﯾ
ﯽ ،
ﻞﻤﺣ ﺧذ ،ﻞﻘﻧو ﯿ هﺮ زﺎﺳ ي رازﺎﺑ و ﯾﺑﺎ ﯽ ﻐﺷ ا ﻞ ﯾ ﻣ دﺎﺠ ﯽ ﺪﻨﮐ . ﻤﻫا ﯿ ﺖ
ﻟد ﻪﺑ تﺎﺒﮐﺮﻣ ﯿ
هدﺮﺘﺴﮔ رﻮﻃ ﻪﺑ ﻪﮐ ﺖﺳا نآ زا عﻮﻨﺘﻣ هدﺎﻔﺘﺳا ﻞ ا
ي
ﻣ ناﻮﻨﻋ ﻪﺑ ﯿ
ﻣ بآ ناﻮﻨﻋ ﻪﺑ ،هزﺎﺗ هﻮ ﯿ
و هﻮ ﯾ دﺎﻨﻗ رد ﺎ ي فﺮﺼﻣ ﺎﻫ
ﻣ ﯽ دﻮﺷ . اراد تﺎﺒﮐﺮﻣ ي
ﺬﭙﻟد ﻢﻌﻃ ﯾ ﮓﻧر ،ﺮ ﺎﻫ ي ﺎﻣورآ و باﺬﺟ
فﺮﺼﻣ ﺪﻨﺴﭘ درﻮﻣ و ﺪﻨﺘﺴﻫ ﻣ راﺮﻗ نﺎﮔﺪﻨﻨﮐ
ﯽ ﮔﯿ ﺪﻧﺮ . ﻨﭽﻤﻫ ﯿ ﻦ
اراد ﯾ شزرﺎ اﺬﻏ ﯾ ﯽ ﮐﺮﺗ و ﻻﺎﺑ ﯿ
ﺰﺗﺎﺒ ﯾ دﺪﻌﺘﻣ لﺎﻌﻓ ﺖﺴ ي
ﻪﻠﻤﺟ زا
ﺳا ﯿ ﺑرﻮﮑﺳا ﺪ ﯿ ﮏ ) وﯾ ﻣﺎﺘ ﯿ ﻦ (C ﺳا ، ﯿ ﺎﻫﺪ ي ﻟﻮﻨﻓ ﯿ ﺋﻮﻧوﻼﻓ ،ﮏ ﯿ
،ﺎﻫﺪ
ﺋﻮﻨﺗورﺎﮐ ﯿ ﺘﮑﭘ ،ﺎﻫﺪ ﯿ ﺎﺳ و ﻦ ﯾ ﮐﺮﺗ ﺮ ﯿ ﻣ تﺎﺒ ﯽ ﺪﻨﺷﺎﺑ . ﻟد ﻪﺑ تﺎﺒﮐﺮﻣ ﯿ
ﻞ
اﻮﺘﺤﻣ ي ﮐﺮﺗ و بآ ﯿ
ﺬﻐﻣ تﺎﺒ ي ﺮﺗﻻﺎﺑ ي هرود لﻮﻃ رد ،ﺪﻧراد ﻪﮐ
ﺑﯿ ﻞﻤﺣ ،ﺖﺷادﺮﺑ ﻦ ﺴﺑ ،فﺮﺼﻣ و ﻞﻘﻧو
ﯿ ﺪﻌﺘﺴﻣ رﺎ ﻪﺑ ﻼﺘﺑا
ﺑ ﻞﻣاﻮﻋ ﻂﺳﻮﺗ ﺖﻧﻮﻔﻋ ﯿ
رﺎﻤ ي از ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ﺪﻨﺘﺴﻫ ] 3 , 4 [.
ﺴﺑ تﺎﺒﮐﺮﻣ ﯿ
ﺑ عاﻮﻧا ﻪﺑ ﻼﺘﺑا ﺪﻌﺘﺴﻣ رﺎ ﯿ
رﺎﻤ ي ﺎﻫ ي ﺖﺷادﺮﺑ زا ﺲﭘ
ز تارﺎﺴﺧ ﺚﻋﺎﺑ ﻪﮐ ﺪﻨﺘﺴﻫ ﯾ
دﺎ ي ﺖﺷادﺮﺑ زا ﺲﭘ ﻪﻠﺣﺮﻣ رد
ﻣ ﯽ دﻮﺷ . اﺮﺑ ﺎﻬﻨﺗ ﻪﻧ ،ﺖﺷادﺮﺑ زا ﺲﭘ ترﺎﺴﺧ ي
ﻪﮑﻠﺑ ،تﺎﺒﮐﺮﻣ
اﺮﺑ ي اﺬﻏ تﻻﻮﺼﺤﻣ مﺎﻤﺗ ﯾ
ﯽ ﻟد ﻪﺑ ، ﯿ ءﻮﺴﻠ ﺬﻐﺗ ﯾ ﻧﺎﻬﺟ ﻪ ﯽ ﺑ ﯿ ﺮﺘﺸ
ﻣ رﻮﺼﺗ و ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﻪﺟﻮﺗ درﻮﻣ ﯽ
ﮔﻮﻠﺟ ﻪﮐ دﻮﺷ ﯿﺮ
ي زا
ﺖﻓا ﯾ ﻧﺎﻬﺟ كﺮﺘﺸﻣ فﺪﻫ ،ﺖﺷادﺮﺑ زا ﺲﭘ ترﺎﺴﺧ ﺎ ﯽ
ﺮﺸﺑ
اﺮﺑ ي ﺘﺳد عﻮﺿﻮﻣ ﯿﺑﺎ
ﯽ ﻧﺎﻬﺟ ﯽ ﻨﻣا ﻪﺑ ﯿ اﺬﻏ ﺖ ﯾ ﯽ ﺎﭘ ﯾ ﺖﺳا راﺪ ]
5 ,
6 [.
ﻢﻬﻣ ﺮﺗ ﯾ ﺴﺑ ﺪﻨﻧﺎﻣ ،ﺖﺷادﺮﺑ زا ﺲﭘ تﺎﺒﮐﺮﻣ ترﺎﺴﺧ ﻦ ﯿ
رﺎ ي زا
ﻣﯿ هﻮ ﺎﻫ ي د ﯾ ﻟد ﻪﺑ ،ﺮﮕ ﯿ
ﺶﻫﺎﮐ ﻞ ﺳﻮﭘ و نزو
ﯿ ﮔﺪ ﯽ ﻪﮐ ﺖﺳا
ﻂﺳﻮﺗ ًﺎﺗﺪﻤﻋ ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ
1مﻮﮑ ) ﺑآ ﮏﭙﮐ ﯽ ( ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ مﻮ
دﯾ ﺠ ﯿ مﻮﺗﺎﺘ ) 2
ﺰﺒﺳ ﮏﭙﮐ ( اﯾ ﻣ دﺎﺠ ﯽ دﻮﺷ . ﺷﺎﻧ ترﺎﺴﺧ عﻮﻤﺠﻣ ﯽ
ا زا ﯾ ﺑ ﻞﻣﺎﻋ ود ﻦ ﯿ
رﺎﻤ ي ﺗﺮﺗ ﻪﺑ از ﯿ ﺎﺗ ﺐ 80 و 30 ﻣ ﺪﺻرد ﯽ ﺪﺳر
] 7 , 8 [.
چرﺎﻗ ﺶﮐ ﺎﻫ ي ﺷ ﯿﻤ ﯿﺎ ﯾ ﯽ ﻮﻟوا ﯾ ﺖ ﺎﻫ ي ﻠﺻا ﯽ ﻟﻮﺗ ﯿ و نﺎﮔﺪﻨﻨﮐﺪ
ﺎﻨﺻ ﯾ ﻪﺘﺴﺑ ﻊ ﺪﻨﺑ ي اﺮﺑ ي نژﻮﺗﺎﭘ ﺖﺷادﺮﺑ زا ﺲﭘ لﺮﺘﻨﮐ ﺪﻨﺘﺴﻫ ﺎﻫ
.
ا ﺎﺑ ﯾ ﺑ هدﺎﻔﺘﺳا ﻪﮐ ﺖﺳا هﺪﺷ شراﺰﮔ لﺎﺣ ﻦ ﯿ
ﺪﺣ زا ﺶ ﯾ ﺎ
1. Penicillium italicum 2. Penicillium digitatum
ﺷ داﻮﻣ زا ﺖﺳردﺎﻧ ﯿﻤ
ﯿﺎ ﯾ ﯽ زروﺎﺸﮐ ي ﻮﺳ ﻪﺑ ﺮﺠﻨﻣ ﯾﻪ
ﺎﻫ ي ﭼرﺎﻗ ﯽ
ﻣ موﺎﻘﻣ ﯽ ﺸﺨﺑﺮﺛا ﻪﮐ دﻮﺷ ﯽ
چرﺎﻗ ﺶﮐ ﻣ ﺶﻫﺎﮐ ار ﺎﻫ ﯽ
ﺎﺑ و ﺪﻫد
ﺪﻨﭼ ﯾ ز عﻮﺿﻮﻣ ﻦ ﯾ
ﺖﺴ ﺤﻣ ﯿ ﻄ ﯽ ﺘﺷاﺪﻬﺑ و ﯽ ﺖﺳا ﻂﺒﺗﺮﻣ .
هوﻼﻋ
ا ﺮﺑ ﯾ ﺎﻘﺑ ،ﻦ ﯾﺎ ي ﺷ داﻮﻣ ﯿﻤ ﯿﺎﯾ ﯽ زروﺎﺸﮐ ي اﺬﻏ داﻮﻣ رد ﯾ
ﯽ عﻮﺿﻮﻣ
ﺚﺤﺑ ﺎﻫ ي ﻣﻮﻤﻋ ﯽ ﻟﻮﺒﻘﻣ ﻪﮐ ﺖﺳا هدﻮﺑ نﺎﻬﺟ رد دﺪﻌﺘﻣ ﯿ
داﻮﻣ ﺖ
ﺷ ﯿﻤ ﯿﺎ ﯾ ﯽ زروﺎﺸﮐ رد ي
داد ﺶﻫﺎﮐ ار .
ﻨﭽﻤﻫ ﯿ ﺖﮐرﺎﻣﺮﭘﻮﺳ ،ﻦ ،ﺎﻫ
ﺖﮐﺮﺷ ﺎﻫ ي ﺮﺷ ﺎﻫرﻮﺸﮐ و تﺎﺒﮐﺮﻣ تاردﺎﺻ ذﺎﺨﺗا ﻪﺑ عو
ﺳ ﯿ ﺖﺳﺎ ﺎﻫ ي ﮕﺘﺨﺳ ﯿ ﻗﺎﺑ درﻮﻣ رد ﻪﻧاﺮ ﯿ
هﺪﻧﺎﻤ ﺎﻫ ي ﺖﻓآ ﺶﮐ ﺎﻫ
هدﺮﮐ ﺪﻧا ] 9 , 10 [ . اﺮﺑﺎﻨﺑ ﯾ ﻧ ،ﻦ ﯿ ﻤﻬﻣ زﺎ ﯽ تﺎﻣاﺪﻗا ﻪﻌﺳﻮﺗ ﻪﺑ
ﺎﺟ ﯾ ﺰﮕ ﯾ ﺳﻮﭘ لﺮﺘﻨﮐ ﻦ ﯿ
ﮔﺪ ﯽ اﺮﺑ ﺖﺷادﺮﺑ زا ﺲﭘ ي
ﺷ داﻮﻣ ﯿﻤ ﯿﺎ ﯾ ﯽ
زروﺎﺸﮐ ي دراد دﻮﺟو .
ﯾ ﻧﺎﻬﺟ ﺪﻧور ﮏ ﯽ
ﺪﺟ ﯾ اﺮﺑ ﺪ ي ﺎﺟ ﻒﺸﮐ ﯾ
ﺰﮕ ﯾ ﻦ ﺎﻫ ﯾ ﯽ ﻪﮐ
ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﺷادﺮﺑ زا ﺲﭘ ﻣ لﺮﺘﻨﮐ ار ﺖ
ﯽ ﻮﻟوا ﺎﺑ ،ﺪﻨﻨﮐ ﯾ
نداد ﺖ
شور ﻪﺑ ﺎﻫ ي ﭘ ﯿ ﮕﺸ ﯿﺮ ي ﺳﻮﭘ زا ﯿ ﮔﺪ ﯽ ﺛﺄﺗ ﻞﻗاﺪﺣ ﺎﺑ ﯿ
ﺖﻣﻼﺳ ﺮﺑ ﺮ
ﺤﻣ و نﺎﺴﻧا ﯿ
ﻂ زﯾ ﺪﭘ ،ﺖﺴ ﯾ ﺖﺳا هﺪﺷ راﺪ .
هﺮﻬﺑ ،ﻊﻗاو رد رادﺮﺑ
ي
ﺧا ﯿ ﺒﻃ تﻻﻮﺼﺤﻣ زا ﺮ ﯿﻌ
ﯽ اﺮﺑ ي ﺑ دﺎﺴﻓ لﺮﺘﻨﮐ ﯿ
ژﻮﻟﻮ ﯾ ﮑ ﯽ و
اﺰﻓا ﯾ راﺪﻬﮕﻧ ﺮﻤﻋ ﺶ ي
ﻧﺪﺷﺪﺳﺎﻓ داﻮﻣ ﯽ
ﺑ ﯿ ﭘ زا ﺶ ﯿ درﻮﻣ ﺶ
ﺮﮔ راﺮﻗ ﻪﺟﻮﺗ ﺖﺳا ﻪﺘﻓ
. و ﻪﺑ ﯾ ﺖﻓآ ،هﮋ ﺶﮐ ﺎﻫ ي ﺒﻃ ﯿﻌ ﯽ ﻨﺘﺒﻣ ﯽ ﺮﺑ
هرﺎﺼﻋ و ﺲﻧﺎﺳا ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ ﻪﺑ ﻆﻓﺎﺤﻣ ناﻮﻨﻋ ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ
ﺎﺟ ﯾ ﺰﮕ ﯾ ﺎﻤﺣ ﻦ ﯾ ﻣ ﺖ ﯽ ﺪﻧﻮﺷ .اﯾ ﮐﺮﺗ ﻦ ﯿ ﺒﻃ تﺎﺒ ﯿﻌ ﯽ ﻠﮐ رﻮﻃ ﻪﺑ ﯽ
ﻪﺑ
ا ناﻮﻨﻋ ﯾ اﺮﺑ ﻦﻤ ي ﺤﻣ ﯿ ز ﻂ ﯾ ﻪﺘﺧﺎﻨﺷ نﺎﺴﻧا ﺖﻣﻼﺳ و ﺖﺴ
ﻣ ﯽ اﺮﺑﺎﻨﺑ ،ﺪﻧﻮﺷ ﯾ
نآ زا هدﺎﻔﺘﺳا ﻪﺑ ﻪﻗﻼﻋ ﻦ زروﺎﺸﮐ فﺪﻫ رد ﺎﻫ
ي
ﺎﭘﯾ اﺰﻓا راﺪ ﯾ ﺸ ﯿ ﺶﻫوﮋﭘ و ﺖﺳا ﻪﺘﻓﺎ ﺎﻫ
ي ز ﯾ دﺎ ي ﺖﺳا هﺪﺷ مﺎﺠﻧا
ﺴﺑ رد ﻪﮐ ﯿ رﺎ ي ﻣ ﺖﺑﺎﺛ دراﻮﻣ زا ﯽ
ﺲﻧﺎﺳا ﻪﮐ ﺪﻨﮐ ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ و
هرﺎﺼﻋ ﺸﻘﻧ ﺖﺳا ﻦﮑﻤﻣ ﺎﻫ ﯽ
وراد داﻮﻣ ناﻮﻨﻋ ﻪﺑ ﯾ
ﯽ هﺪﻧراﺪﻬﮕﻧ و
اﺬﻏ داﻮﻣ ﯾﯿ ﺪﻨﺷﺎﺑ ﻪﺘﺷاﺪ ]
11 - 14 [.
نﺎﻨﺷا هﺎﯿﮔ (Seidlitziarosmarinus)
داﻮﻧﺎﺧ ﻪﺑ ﻖﻠﻌﺘﻣ ه
Chenopodiaceae د ﻪﮐ ﺖﺳا
رﺎﺸﺘﻧا ﻞﺑﺎﻗ رﻮﺷ ﻖﻃﺎﻨﻣ ر
ﻣ ﯽ ﺪﺷﺎﺑ . د هﺎﯿﮔ ﻦﯾا ﺪﻨﻔﺳﻮﮔ ﯽﯾاﺬﻏ هﺮﯿﺟ ر دﺎﻔﺘﺳا ﺮﺘﺷ و
ﺪﺷ ه و ه
د د ﯽﻤﻬﻣ ﺶﻘﻧ ﺮﯾﻮﮐ ﻪﯿﺷﺎﺣ ر ﺎﻔﯾا كﺎﺧ زا ﺖﻇﺎﻔﺣ ر
ﻣ ﯽ ﺪﻨﮐ ] 15 [.
ﺧﺮﺑ ﯽ شراﺰﮔ ﮐﺎﺣ ﺎﻫ ﯽ ا ﻪﮐ ﺖﺳا نآ زا ﯾ
ﮔ ﻦ ﯿ هﺎ
اراد ي وراد صاﻮﺧ ﯾ
ﯽ ژﻮﻟﻮﺗﺎﻣرد رد و هدﻮﺑ ي
ﻣ هدﺎﻔﺘﺳا ﯽ دﻮﺷ .
ﻗﺎﺑﺮﺘﺴﮐﺎﺧ ﯽ گﺮﺑ نﺪﻧازﻮﺳ زا ﺲﭘ هﺪﻧﺎﻣ ﻪﻗﺎﺳ و ﺎﻫ
اراد ﺎﻫ ي ﺮﺛا
ﻧﻮﻔﻋ ﺪﺿ ﯽ ﺮﺘﮐﺎﺑ ﺪﺿ و هﺪﻨﻨﮐ ﯾﺎﯾ
ﯽ ﺖﺳا . ﺎﻬﺘﻓﺎﺑ ي ر ﯾ ﮔ ﻪﺸ ﯿ هﺎ
ﻓﺮﻇ نﺎﻨﺷا ﯿ ﻻﺎﺑ ﺖ ﯾ ﯽ دﺎﻘﻣ بﺬﺟ رد ﯾ
ز ﺮ ﯾ دﺎ ي ﻠﻗ داﻮﻣ ﯿﺎ ﯾ ﯽ كﺎﺧ
ﺪﺳ ﺪﻨﻧﺎﻣ ﯾ ﺳﺎﺘﭘ و ﻢ ﯿ ﺪﻧراد ﻢ . ﻨﭽﻤﻫ ﯿ ا ﻪﮐ ﺖﺳا هﺪﺷ شراﺰﮔ ﻦ ﯾ
ﻦ
ﮔﯿ اراد هﺎ ي وراد صاﻮﺧ ﯾ
ﯽ و هدﻮﺑ اﺮﺑ ي ﺧﺮﺑ نﺎﻣرد ﯽ
ﻪﻨﮐآ ﺎﻫ
ﻣ هدﺎﻔﺘﺳا ﯽ دﻮﺷ ] 16 [.
ا زا فﺪﻫ ﯾ ﻫ هرﺎﺼﻋ جاﺮﺨﺘﺳا ،ﺶﻫوﮋﭘ ﻦ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ نﺎﻨﺷا هﺎ
ﺳرﺮﺑ و ﯽ ﻣﺪﺿ صاﻮﺧ ﯿ
ﺑوﺮﮑ ﯽ چرﺎﻗ ﺮﺑاﺮﺑ رد نآ ﺎﻫ
ي ﻞﻣﺎﻋ
ﺳﻮﭘ ﯿ ﮔﺪ ﯽ ﻣ لﺎﻘﺗﺮﭘ ﯽ ﺪﺷﺎﺑ . ا ﺮﺑ هوﻼﻋ ﯾ
ﻣ ،ﻦ ﯿ و ﻞﮐ لﻮﻨﻓ ناﺰ
ﺋﻮﻧوﻼﻓ ﯿ ﺘﻧآ ﺮﺛا و ﻞﮐ ﺪ ﯽ
ﺴﮐا ﯿ ﻧاﺪ ﯽ ا ﯾ ﺳرﺮﺑ هرﺎﺼﻋ ﻦ ﯽ
دﺮﮔ ﯾﺪ .
2 - شور و داﻮﻣ ﺎﻫ
2 - 1 - داﻮﻣ
ﻟﯿ ﺌﻟﻮﻨ ﯿ ﺳا ﮏ ﯿ ﻟﻮﻓ فﺮﻌﻣ ،ﺪ ﯿ
ﻦ - ﺳ ﯿ دار ،ﻮﺘﻟﺎﮐﻮ ﯾ
دازآ لﺎﮑ ABTS3
،
دار ﯾ دازآ لﺎﮑ DPPH4
ﺳ ﺖﮐﺮﺷ زا ﯿ
ﺎﻤﮕ ) ﺮﻣآ ﯾ ﺎﮑ ( ﺤﻣ و ﯿ ﻂ ﺎﻫ ي
زا ثاﺮﺑ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ و رﺎﮔآ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ ﺖﺸﮐ كﺮﻣ ﺖﮐﺮﺷ )
نﺎﻤﻟآ ( ﻬﺗ ﯿ ﺪﻧﺪﺷ ﻪ .
2 - 2 - هرﺎﺼﻋ جاﺮﺨﺘﺳا
ﮔﯿ هﺎ ﺎﺳ رد نﺎﻨﺷا ﯾ
ﺳآ ﻂﺳﻮﺗ و ﮏﺸﺧ ﻪ ﯿ
ﺎﻣزآ بﺎ ﯾ ﻫﺎﮕﺸ ﯽ ردﻮﭘ
ﺪﻧﺪﺷ . ﮔ ردﻮﭘ ،ﻪﻣادا رد ﯿ
لﻮﻧﺎﺗا لﻼﺣ ﻪﺑ هﺎ )
50 ﺪﺻرد ( ﻪﻓﺎﺿا
د و ﺪﺷ ﯾ ﺳﺮﭙﺴ ﯿ تﺪﻣ ﻪﺑ نﻮ 48
ﺎﻣد رد ﺖﻋﺎﺳ ي
25 ﻪﺟرد
ﺘﻧﺎﺳ ﯽ ور و داﺮﮔ ي
ﺷ هﺎﮕﺘﺳد ﯿ
ﺪﺷ هداد نﺎﮑﺗ ﺮﮑ .
ﮔ ردﻮﭘ ﯿ هﺎ
ﻓﺎﺻ ﺬﻏﺎﮐ ﻂﺳﻮﺗ ﯽ
زﺎﺳاﺪﺟ هرﺎﺼﻋ زا ي
دﺮﮔ ﯾ هزﺎﺗ لﻼﺣ و ﺪ
ور ي ﺎﻫردﻮﭘ ي ﮔ ﯿ ر هﺎ ﯾ ﮔ هرﺎﺼﻋ و ﺪﺷ ﻪﺘﺨ ﯿﺮ
ي تﺪﻣ ﻪﺑ 24
د ﺖﻋﺎﺳ ﯾ دﺮﮔ راﺮﮑﺗ ﺮﮕ ﯾﺪ
. هرﺎﺼﻋ ﺪﻤﻫ ﺎﺑ ﺎﻫ ﯾ ﮐﺮﺗ ﺮﮕ ﯿ و ﺪﻧﺪﺷ ﺐ
ﺎﻬﻧ لﻮﻠﺤﻣ ﯾ
ﯽ رﺎﺗور هﺎﮕﺘﺳد ﻂﺳﻮﺗ ي
ﺎﻣد رد ﻸﺧ ﺖﺤﺗ و ي
40
رد ﺘﻧﺎﺳ ﻪﺟ ﯽ ﺨﺒﺗ رﻮﻈﻨﻣ ﻪﺑ داﺮﮔ ﯿ
ﺪﺷ هداد تراﺮﺣ لﻼﺣ ﺮ .
رد
ﺎﻬﻧ ﯾ ﻠﻐﺗ هرﺎﺼﻋ ندﺮﮐ ﮏﺸﺧ ﺖﻬﺟ نوآ زا ﺖ ﯿ
هدﺎﻔﺘﺳا هﺪﺷ ﻆ
دﺮﮔ ﯾ ﺪ ) 50 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
،داﺮﮔ 3 زور ( ] 17 [ .
2 - 3 - ﻣ ﯿ ﻞﮐ لﻮﻨﻓ ناﺰ
ﻌﺗ ﺖﻬﺟ ﯿﯿ ﻣ ﻦ ﯿ ،هرﺎﺼﻋ ﻞﮐ لﻮﻨﻓ ناﺰ 100
ﻣ ﯿﻠ ﯽ ﻟﯿ ﺎﺑ نآ زا ﺮﺘ 5/
2
ﻣﯿ ﻠ ﯽ ﻟﯿ ﻟﻮﻓ فﺮﻌﻣ زا ﺮﺘ ﯿ
ﻦ - ﺳ ﯿ ﻮﺘﻟﺎﮐﻮ ) ﻗر ﺖ 10 ﺮﺑاﺮﺑ ( ﺪﺷ طﻮﻠﺨﻣ
تﺪﻣ ﻪﺑ ﺶﻨﮐاو و 5
ﻗد ﯿ دﺮﮔ مﺎﺠﻧﺎﻬﻘ ﯾﺪ
. ﺲﭙﺳ 5/
2 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ
لﻮﻠﺤﻣ Na2CO3
ﺎﻬﻧ لﻮﻠﺤﻣ و ﺪﺷ ﻪﻓﺎﺿا نآ ﻪﺑ عﺎﺒﺷا ﯾ
ﯽ ﻪﺑ
تﺪﻣ 1 ﺎﻣد رد ﺖﻋﺎﺳ ي
ﺤﻣ ﯿ راﺪﻬﮕﻧ ﻂ ي
دﺮﮔ ﯾﺪ . ﺎﻬﻧ رد ﯾ ﺖ
رد ﺶﻨﮐاو طﻮﻠﺨﻣ بﺬﺟ 725
اﻮﺘﺤﻣ و دﻮﺷ هﺪﻧاﻮﺧ ﺮﺘﻣﻮﻧﺎﻧ ي
ﻠﭘ ﯽ ﻣ ترﻮﺻ ﻪﺑ هرﺎﺼﻌﻠﮐ لﻮﻨﻓ ﯿﻠ
ﯽ ﺳا لدﺎﻌﻣ مﺮﮔ ﯿ
ﻟﺎﮔ ﺪ ﯿ رد ﮏ
ﮔ ﮏﺸﺧ هدﺎﻣ مﺮﮔ ﺮﻫ ﯿ
هﺎ ) mg GAE/g (
ﺑﯿ ﺪﺷ نﺎ ] 18 [.
3 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) 4 2,2-diphenyl-1-picrylhydrazyl
2 - 4 - ﻣ ﯿ ﺋﻮﻧوﻼﻓ ناﺰ ﯿ
ﻞﮐ ﺪ
،ﻪﺻﻼﺧ رﻮﻃ ﻪﺑ 5/
2 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺎﺑ هرﺎﺼﻋ زا ﺮﺘ 5/
2 ﻣ ﯿﻠ ﯽ ﻟﯿ فﺮﻌﻣ ﺮﺘ
AlCl3
لﻮﻧﺎﺗا رد 90
تﺪﻣ ﻪﺑ و ﺪﺷ طﻮﻠﺨﻣ ﺪﺻرد 40
ﻗد ﯿ ﻪﻘ
ﺎﻣد رد ي راﺪﻬﮕﻧ قﺎﺗا ي
دﺮﮔ ﯾﺪ . رد طﻮﻠﺨﻣ بﺬﺟ ،نآ زا ﺲﭘ
لﻮﻃ جﻮﻣ 415 هزاﺪﻧا ﺮﺘﻣﻮﺗﻮﻓوﺮﺘﮑﭙﺳا زا هدﺎﻔﺘﺳا ﺎﺑ ﺮﺘﻣﻮﻧﺎﻧ ﮔﯿ
ﺮ ي
ﺪﺷ . ﺘﺳﺮﺋﻮﮐ زا ﯿ ﮐﺮﺗ ناﻮﻨﻋ ﻪﺑ ﻦ ﯿ
ﺪﺷ هدﺎﻔﺘﺳا دراﺪﻧﺎﺘﺳا ﺐ .
ﻪﻤﻫ
ﺎﻣزآ ﯾ ﺶ اﻮﺘﺤﻣ و ﺪﺷ مﺎﺠﻧا راﺮﮑﺗ ﻪﺳ رد ﺎﻫ ي
ﺋﻮﻧوﻼﻓ ﯿ ﻞﮐ ﺪ
اﺮﺑ ي ﻣ ترﻮﺻ ﻪﺑ هرﺎﺼﻋ ﯿﻠ
ﯽ ﺘﺳﺮﺋﻮﮐ لدﺎﻌﻣ مﺮﮔ ﯿ
مﺮﮔ ﺮﻫ رد ﻦ
) mg QE/g (
ﻨﺤﻨﻣ سﺎﺳا ﺮﺑ هرﺎﺼﻋ ﯽ
ﻟﺎﮐ ﯿﺒ ﺳاﺮ ﯿ ﻄﺧ نﻮ ﯽ
ﺘﺳﺮﺋﻮﮐ ﯿ ﻌﺗ ﻦ ﯿﯿ ﺪﺷ ﻦ ] 19 [.
2 - 5 - ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ
ﺻﺎﺧ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ شور ﻖﺑﺎﻄﻣ نﺎﻨﺷا
ﻠﻋ ﯿ ﻧﺎﻬﺒﻬﺑ هداﺰ ﯽ
نارﺎﮑﻤﻫ و )
2019 ( ﻠﺑﺎﻗ سﺎﺳا ﺮﺑ و ﯿ
رد نآ ﺖ
دار رﺎﻬﻣ ﯾ لﺎﮑ ﺎﻫ ي دازآ وDPPH ﺳرﺮﺑABTS
ﯽ دﺮﮔ ﯾ ﺪ ] 20 [.
2 - 5 - 1 - ﻟﺎﻌﻓ ﯿ دار رﺎﻬﻣ ﺖ ﯾ
دازآ لﺎﮑ
DPPH100 ﻣ ﯿ ﻟوﺮﮑ ﯿ هرﺎﺼﻋ ﺮﺘ ﯾ
ﺎﺑ ﺪﻫﺎﺷ ﺎ 9/
3 ﻣ ﯿﻠ ﯽ ﻟﯿ ﻟﻮﻧﺎﺗا لﻮﻠﺤﻣ زا ﺮﺘ ﯽ
دار ﯾ ﻟﺎﮑ ﯽ )DPPH 12 /0 ﻣ ﯿﻠ ﯽ رﻻﻮﻣ ( ﺪﺷ طﻮﻠﺨﻣ .
لﻮﻠﺤﻣ
ﻪﺑ ﺖﺳد تﺪﻣ ﻪﺑ هﺪﻣآ 30
ﻗد ﯿ ﺎﻣد رد ﻪﻘ ي
ﺤﻣ ﯿ ﻧﺎﮑﻣ رد ﻮﻄ ﯽ
رﺎﺗ ﯾ راﺪﻬﮕﻧ ﮏ ي
لﻮﻃ رد نآ بﺬﺟ و ﺪﺷ جﻮﻣ
517 ﺮﺘﻣﻮﻧﺎﻧ
دﺮﮔ ﺖﺋاﺮﻗ ﯾﺪ
. ﻟﺎﻌﻓ ﺲﭙﺳ ﯿ دار رﺎﻬﻣ ﺖ ﯾ
دازآ لﺎﮑ هرﺎﺼﻋDPPH
ز ترﻮﺻ ﻪﺑ ﯾ
هزاﺪﻧا ﺮ ﮔﯿ ﺮ ي ﺪﺷ :
Inhibition (%) = [(Ablank-Asample)/Asample] × 100
2 - 5 - 2 - ﻟﺎﻌﻓ ﯿ دار رﺎﻬﻣ ﺖ ﯾ
دازآ لﺎﮑ
ABTSﺗﺎﮐ ﯿ نﻮ ﺎﻫ ي ABTS ﻢﺠﺣ ﺶﻨﮐاو ﺎﺑ اﺪﺘﺑا ﯾ
لﻮﻠﺤﻣ نﺎﺴﮑ 7/
0
ﻣﯿ ﻠ ﯽ رﻻﻮﻣ لﻮﻠﺤﻣ و ABTS
45 /2 ﻣ ﯿﻠ ﯽ ﺳﺎﺘﭘ رﻻﻮﻣ ﯿ
ﻢ
راﺪﻬﮕﻧ زا ﺲﭘ تﺎﻔﻟﻮﺳﺮﭘ ي
ﻪﺑ لﻮﻠﺤﻣ ﺖﺳد
ﻣآ ﺎﻣد رد هﺪ ي
اﺮﺷ رد و قﺎﺗا ﯾ
رﺎﺗ ﻂ ﯾ تﺪﻣ ﻪﺑ ﮏ 16
ﻟﻮﺗ ،ﺖﻋﺎﺳ ﯿ
ﺪﻧﺪﺷ ﺪ .
لﻮﻠﺤﻣ ﺲﭙﺳ ABTS
ﻗر لﻮﻧﺎﺘﻣ ﺎﺑ ﯿ
بﺬﺟ ﻪﺑ ﺎﺗ ﺪﺷ ﻖ
2/
0 7/± 0 لﻮﻃ رد جﻮﻣ 734 ﺪﺳﺮﺑ ﺮﺘﻣﻮﻧﺎﻧ .
،نآ زا ﺲﭘ 9/
3
ﻣﯿ ﻠ ﯽ ﻟﯿ ﻗر لﻮﻠﺤﻣ زا ﺮﺘ ﯿ
دار هﺪﺷ ﻖ ﯾ
ﻟﺎﮑ ﯽ ﺎﺑ 100 ﻣ ﯿ ﻟوﺮﮑ ﯿ هرﺎﺼﻋ ﺮﺘ
ﯾ لﻮﻧﺎﺘﻣ ﺎ ) ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ناﻮﻨﻋ ﻪﺑ (
ﺪﺷ طﻮﻠﺨﻣ .
ﻮﻠﺤﻣ تﺪﻣ ﻪﺑ ل
6 ﻗد ﯿ ﺎﻣد رد ﻪﻘ ي ﺤﻣ ﯿ راﺪﻬﮕﻧ ﻂ ي دﺮﮔ ﯾ لﻮﻃ رد بﺬﺟ و ﺪ جﻮﻣ
734 ﺪﺷ ﺖﺋاﺮﻗ ﺮﺘﻣﻮﻧﺎﻧ .
ﻟﺎﻌﻓ ﯿ دار رﺎﻬﻣ ﺖ ﯾ
دازآ لﺎﮑ
ز ﻪﻄﺑار ﻖﺒﻃ هرﺎﺼﻋDPPH ﯾ
ﺪﺷ ﻪﺒﺳﺎﺤﻣ ﺮ :
Inhibition (%) = [(Ablank-Asample)/Asample] × 100
ﻟﺎﻌﻓ ﻪﮐ ﺖﺳا ﺮﮐذ ﻪﺑ مزﻻ ﯿ
ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺎﻬﻧ ﯾ ﯽ ﺮﺑ هرﺎﺼﻋ
ﺐﺴﺣ IC50
)ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ ( دﺮﮔ شراﺰﮔ ﯾﺪ
.
2 - 6 - ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﺳرﺮﺑ رﻮﻈﻨﻣ ﻪﺑ ﯽ
ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ هﺎ
ﺮﺑاﺮﺑ رد نﺎﻨﺷا ﻮﺳ
ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﻨﭘ ﯽ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ و
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ شور زا ، ﺎﻫ ي ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ د ﯾ د ﮏﺴ ﯾﻔ ﯿ نژﻮ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ،رﺎﮔآ ﯽ
ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و
ﮔﺪﻨﺸﮐ ﯽ ، ﺘﻤﺣر شور ﻖﺑﺎﻄﻣ ﯽ
ﻨﺟ ﯿ ﻠﻋ و دﺎﺑآﺪ ﯿ
ﻧﺎﻬﺒﻬﺑ هداﺰ ﯽ
) 1400 ( دﺮﮔ هدﺎﻔﺘﺳا ﯾ
ﺪ ] 21 [.
2 - 6 - 1 - د ﯾ د ﮏﺴ ﯾﻔ ﯿ رﺎﮔآ نژﻮ
اﺮﺑ ي ا ﯾ هرﺎﺼﻋ اﺪﺘﺑا ،رﻮﻈﻨﻣ ﻦ )
30 ، 60 ، 90 و 120 ﻣ ﯿﻠ ﯽ رد مﺮﮔ
ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ( ﻓ ﻂﺳﻮﺗ ﯿ ﺎﺸﻏ ﺮﺘﻠ ﯾ ﯽ ﺎﻨﻣ ﺮﻄﻗ ﺎﺑ ﺬﻓ
22 /0 ﻣ ﯿ نوﺮﮑ
ﺮﺘﺳا ﯾ دﺮﮔ ﻞ ﯾﺪ . د ،ﻪﻣادا رد ﯾ
ﮏﺴ ﺎﻫ ي ﺮﺘﺳا ﯾ تﺪﻣ ﻪﺑ ﻞ 15
ﻗد ﯿ ﻪﻘ
ﻪﻃﻮﻏ هرﺎﺼﻋ رد ور ﺲﭙﺳ و رو
ي ﺤﻣ ﺢﻄﺳ ﯿ زوﺮﺑﺎﺳ ﺖﺸﮐ ﻂ
ﺮﺘﺳا رﺎﮔآ زوﺮﺘﺴﮐد ﯾ
ًﻼﺒﻗ ﻪﮐ ،ﻞ 100
ﻣ ﯿ ﻟوﺮﮑ ﯿ ﻮﺳ ﺮﻫ زا ﺮﺘ ﯾ
ﻪ
ﭼرﺎﻗ ﯽ ور ي ﺪﻧﺪﺷ هداد راﺮﻗ ،دﻮﺑ هﺪﺷ هداد ﺖﺸﮐ نآ .
ﺲﭙﺳ
ﺤﻣ ﯿ تﺪﻣ ﻪﺑ ﺖﺸﮐ ﻂ 72
ﺎﻣد رد ﺖﻋﺎﺳ ي
27 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
داﺮﮔ
راﺪﻬﮕﻧ ي ﺎﻬﻧ رد و ﺪﺷ ﯾ
ﻪﻟﺎﻫ ﺮﻄﻗ ﺖ ﻣ ﺐﺴﺣ ﺮﺑ ﺪﺷر مﺪﻋ
ﯿﻠ ﯽ ﺮﺘﻣ
هزاﺪﻧا ﮔﯿ ﺮ ي ﻟﺎﻌﻓ ناﻮﻨﻋ ﻪﺑ و ﯿ
ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
شراﺰﮔ هرﺎﺼﻋ
دﺮﮔ ﯾﺪ .
2 - 6 - 2 - رﺎﮔآ رد ﮏﻫﺎﭼ رﺎﺸﺘﻧا
ا رد ﯾ ﭼرﺎﻗﺪﺿ شور ﻦ ﯽ
ﮏﻫﺎﭼ اﺪﺘﺑا ، ﺎﻫ
ﯾ ﯽ ﺺﺨﺸﻣ ﺮﻄﻗ ﺎﺑ
ور ي ﺤﻣ ﺢﻄﺳ ﯿ ا رﺎﮔآ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ ﻂ ﯾ
ﺲﭙﺳ و ﺪﻧﺪﺷ دﺎﺠ
ﺎﺑ 20 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﺘﺳا هرﺎﺼﻋ زا ﺮﺘ ﯾ
ﻞ ) 30 ، 60 ، 90 و 120
ﻣﯿ ﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﻮﺳ و ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ﺪﻧﺪﺷ ﺮﭘ . ،ﻪﻣادا رد
ﺤﻣ ﯿ تﺪﻣ ﻪﺑ ﺖﺸﮐ ﻂ 72
ﺎﻣد رد ﺖﻋﺎﺳ ي
27 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
داﺮﮔ
راﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ ي
ﺎﻬﻧ رد و ﺪﺷ ﯾ
ﺮﻫ فاﺮﻃا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﺖ
ﻣ ﺐﺴﺣ ﺮﺑ ﮏﻫﺎﭼ ﯿﻠ
ﯽ هزاﺪﻧا ﺮﺘﻣ ﮔﯿ ﺮ ي دﺮﮔ ﯾﺪ .
2 - 6 - 3 - ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
اﺮﺑ ي ا مﺎﺠﻧا ﯾ ﭼرﺎﻗ ﺪﺿ شور ﻦ ﯽ
، 100 ﻣ ﯿ ﻟوﺮﮑ ﯿ زا ﺮﺘ
ﺴﻧﺎﭙﺳﻮﺳ ﯿ ﻣ نﻮ ﯿ ﺑوﺮﮑ ﯽ ﻪﻟﻮﻟ رد ﺎﻫ ي ﺎﻣزآ ﯾ ر ﺶ ﯾ ﺲﭙﺳ و ﺪﺷ ﻪﺘﺨ
5 ﻣﯿ ﻠ ﯽ ﻟﯿ ﺖﻈﻠﻏ زا ﺮﺘ ﺎﻫ
ي هرﺎﺼﻋ ﻒﻠﺘﺨﻣ )
4 ، 8 ، 16 ، 32 ، 64 ،
128 ، 256 و 512 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( دﺮﮔ ﻪﻓﺎﺿا نآ ﻪﺑ ﯾﺪ
.
ﻮﻟ ﻪﻟ ﺎﻫ ي ﺎﻣزآ ﯾ تﺪﻣ ﻪﺑ ﺶ 72
ﺎﻣد رد ﺖﻋﺎﺳ ي
27 ﻪﺟرد
ﺘﻧﺎﺳ ﯽ راﺪﻬﮕﻧ داﺮﮔ ي
ﺪﻧﺪﺷ . ﺳرﺮﺑ ﺎﺑ ﯽ ﻮﺳ ﺪﺷر ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ا تروﺪﮐ ﻂﺳﻮﺗ
ﯾ ﺘﻈﻠﻏ ،ﻢﺸﭼ ﺎﺑ هﺪﺷ دﺎﺠ ﯽ
ﻪﮐ هرﺎﺼﻋ زا
ﻫﯿ ﺗروﺪﮐ ﻪﻧﻮﮕﭽ ﯽ
ﺤﻣ رد ﯿ ا ﻂ ﯾ ﻞﻗاﺪﺣ ناﻮﻨﻋ ﻪﺑ ،دﻮﺑ هدﺮﮑﻧ دﺎﺠ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﯽ
دﺮﮔ شراﺰﮔ ﯾﺪ
.
2 - 6 - 4 - ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
ا رد ﯾ ،نﻮﻣزآ ﻦ 100
ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺤﻣ زا ﺮﺘ ﯿ تروﺪﮐ ﺪﻗﺎﻓ ﺖﺸﮐ ﻂ
ور ي ﺢﻄﺳ ﺤﻣ ﯿ هداد ﺖﺸﮐ رﺎﮔآ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ ﺖﺸﮐ ﻂ
ﺪﺷ . راﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ ي
تﺪﻣ ﻪﺑ 72
ﺎﻣد رد ﺖﻋﺎﺳ ي
27 ﻪﺟرد
ﺘﻧﺎﺳ ﯽ دﺮﮔ مﺎﺠﻧا داﺮﮔ ﯾ
ﺘﻈﻠﻏ ﻞﻗاﺪﺣ ﺲﭙﺳ و ﺪ ﯽ
ﺐﺒﺳ ﻪﮐ
ﮔﻮﻠﺟ ﯿﺮ ي ﻮﺳ ﺪﺷر زا ﯾﻪ
ﺎﻫ ي ﭼرﺎﻗ ﯽ دﺮﮔ ﯾ ﻞﻗاﺪﺣ ناﻮﻨﻋ ﻪﺑ ،ﺪ
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﯽ
ﻌﺗ هرﺎﺼﻋ ﯿﯿ ﺪﺷ شراﺰﮔ و ﻦ .
2 - 7 - ﻟﺎﻧآ ﯿ رﺎﻣآ ﺰ ي
ﻠﮐ ﯿ ﺎﻣزآ ﻪ ﯾ ﺶ مﺮﻧ زا و ﺪﻧﺪﺷ مﺎﺠﻧا راﺮﮑﺗ ﻪﺳ رد ﺎﻫ راﺰﻓا
وSPSS
ﻪﻨﻣاد ﺪﻨﭼ نﻮﻣزآ ا
ي ﻦﮑﻧاد ) 05 /0
<
(p ﻟﺎﻧآ ﻖﺑﺎﻄﻣ ﯿ راو ﺰ ﯾ ﺲﻧﺎ
ﯾ ﮏ ﻟﺎﻧآ ﺖﻬﺟ ﻪﻓﺮﻃ ﯿ
هداد ﺰ ﺎﻫ ي ا ﯾ دﺮﮔ هدﺎﻔﺘﺳا ﻪﻌﻟﺎﻄﻣ ﻦ ﯾﺪ
.
3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ
ﺎﺘﻧ ﯾ اﻮﺘﺤﻣ ﺞ ي ﺋﻮﻧوﻼﻓ و ﻞﮐ لﻮﻨﻓ ﯿ
ﻫ هرﺎﺼﻋ ﻞﮐ ﺪ ﯿ
ﻠﮑﻟاورﺪ ﯽ
ﮔﯿ ﻞﮑﺷ رد نﺎﻨﺷا هﺎ 1
ﺖﺳا هﺪﺷ شراﺰﮔ .
ﻣﯿ و ﻞﮐ لﻮﻨﻓ ناﺰ
ﺋﻮﻧوﻼﻓ ﯿ ﺗﺮﺗ ﻪﺑ هرﺎﺼﻋ ﻞﮐ ﺪ ﯿ
ﺎﺑ ﺮﺑاﺮﺑ ﺐ mg GAE/g
24 /0
±
19 / 26 و mg QE/g 17
/0 1± / 11 دﻮﺑ .
Fig 1 Total phenols and total flavonoids content of Seidlitziarosmarinus hydroalcoholic extract.
ﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯾ
ﻟﺎﻌﻓ ﺞ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ) ﻞﮑﺷ 2 ( هرﺎﺼﻋ ،
ﻫﯿ ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ دار رﺎﻬﻣ ﻪﺑ ردﺎﻗ نﺎﻨﺷا هﺎ ﯾ
لﺎﮑ ﺎﻫ ي دازآ
)DPPH 36 /0 14 ± / 112 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ ( و
)ABTS 47 /0 46± / 103 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ ( دﻮﺑ .
Fig 2 Antioxidant activity of Seidlitziarosmarinus hydroalcoholic extract.
ﺎﺘﻧ ﯾ ﻟﺎﻌﻓ ﺞ ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ ﺎﭘ ﺮﺑ نﺎﻨﺷا هﺎ ﯾ
ﻪ
د شور ﯾ د ﮏﺴ ﯾﻔ ﯿ ﻞﮑﺷ رد رﺎﮔآ نژﻮ 3
ﺖﺳا هﺪﺷ ﻪﺋارا .
ﺪﺿ ﺮﺛا
ﭼرﺎﻗ ﯽ دﻮﺑ ﺖﻈﻠﻏ ﻪﺑ ﻪﺘﺴﺑاو هرﺎﺼﻋ .
اﺰﻓا ﯾ زا هرﺎﺼﻋ ﺖﻈﻠﻏ ﺶ
30 ﻪﺑ 120 ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ اﺰﻓا ﺐﺒﺳ ﺮﺘ ﯾ
ﻨﻌﻣ ﺶ ﯽ ﺮﻄﻗ راد
زا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ 2/
7 ﻣ ﯿﻠ ﯽ ﻪﺑ ﺮﺘﻣ 5/
14 ﻣ ﯿﻠ ﯽ اﺮﺑ ﺮﺘﻣ ي چرﺎﻗ
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ و 6/
6 ﻣ ﯿﻠ ﯽ ﻪﺑ ﺮﺘﻣ 13 ﻣ ﯿﻠ ﯽ اﺮﺑ ﺮﺘﻣ ي چرﺎﻗ
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ دﺮﮔ ﯾﺪ . ا ﺮﺑ هوﻼﻋ ﯾ
ﻮﺳ ،ﻦ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ
ﺗوﺎﻔﺘﻣ رﺎﺘﻓر ﯽ
ﭼرﺎﻗ ﺪﺿ ﺮﺛا ﻪﺑ ﺖﺒﺴﻧ ﯽ
و ﺪﻧداد نﺎﺸﻧ هرﺎﺼﻋ
ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﺳﺎﺴﺣ ﯿ ﺑ ﺖ ﯿ ﺮﺘﺸ ي ﻪﺑ ﺖﺒﺴﻧ ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ مﻮ
اﯾ ﻟﺎﺘ ﯿ مﻮﮑ داد نﺎﺸﻧ .
Fig 3 Antifungal activity of Seidlitziarosmarinus hydroalcoholic extract, based on disk diffusion
agar.
ﺎﺘﻧ
ﯾ ﻧ رﺎﮔآ ﮏﻫﺎﭼ نﻮﻣزآ ﺞ ﯿ
ﺎﺘﺳار رد ﺰ ي
نﻮﻣزآ تاﺪﻫﺎﺸﻣ
دﯾ د ﮏﺴ ﯾﻔ ﯿ دﻮﺑ رﺎﮔآ نژﻮ )
ﻞﮑﺷ 4 .(
رﻮﻄﺑ ﯾ مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻪﮑ
اﺰﻓا ﺎﺑ ﺪﺷر ﯾ
ﻨﻌﻣ رﻮﻄﺑ هرﺎﺼﻋ ﺖﻈﻠﻏ ﺶ ﯽ
راد ي اﺰﻓا ﯾ ﺶ ﯾ و ﺖﻓﺎ
چرﺎﻗ ﻨﭘ ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻪﺑ ﺖﺒﺴﻧ ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ رد
سﺎﺴﺣ هرﺎﺼﻋ ﺮﺑاﺮﺑ دﻮﺑ ﺮﺗ
. ا رد ﯾ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ،ﺎﺘﺳار ﻦ
اﺮﺑ ي ﻮﺳ ﯾﻪ ﺎﻫ ﻨﭘ ي ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘ و ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ رد
ﺖﻈﻠﻏ رﻮﻀﺣ 120
ﻣ ﯿﻠ ﯽ ﻣ رد مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺗﺮﺗ ﻪﺑ هرﺎﺼﻋ ﺮﺘ ﯿ
ﺮﺑاﺮﺑ ﺐ
ﺎﺑ 3/
15 و 4/
13 ﻣ ﯿﻠ ﯽ دﻮﺑ ﺮﺘﻣ .
Fig 4 Antifungal activity of Seidlitziarosmarinus hydroalcoholic extract, based on well diffusion
agar.
رﺎﮔآ ﮏﻫﺎﭼ نﻮﻣزآ رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻪﮐ ﺖﺳا ﺮﮐذ ﻪﺑ مزﻻ گرﺰﺑ د نﻮﻣزآ زا ﺮﺗ ﯾ
د ﮏﺴ ﯾﻔ ﯿ دﻮﺑ رﺎﮔآ نژﻮ .ا
ﯾ ﻦﮑﻤﻣ ﺖﻟﺎﺣ ﻦ
ﻟد ﻪﺑ ﺖﺳا ﯿ
ﻘﺘﺴﻣ سﺎﻤﺘﻠ ﯿ
ﺑ ﻢ ﯿ ﻣ و هرﺎﺼﻌﻨ ﯿ
ﻧﺎﮔراوﺮﮑ ﯿ شور رد ﻢﺴ
ﺪﺷﺎﺑ رﺎﮔآ ﮏﻫﺎﭼ .
ﻟﺎﺣ رد ﯽ د ﺢﻄﺳ زا هرﺎﺼﻋ رﺎﺸﺘﻧا ،ﻪﮐ ﯾ
ﮏﺴ
ﺤﻣ ﻪﺑ ﯿ ﭼرﺎﻗ ﺮﺛا ،ﻂ ﯽ
ﺪﺷور رد ار نآ ﯾ
د ﮏﺴ ﯾﻔ ﯿ ﻌﺗ رﺎﮔآ نژﻮ ﯿﯿ
ﻦ
ﻣ ﯽ ﺪﻨﮐ ] 12 - 14 , 20 - 24 [.
ﺎﺘﻧ ﯾ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ نﻮﻣزآ ﺞ ﯽ
ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و
ﮔﺪﻨﺸﮐ ﯽ لوﺪﺟ رد 1
ﺖﺳا هﺪﺷ ﻪﺋارا .
ﻮﺳ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ﺎﭘ ﺖﻈﻠﻏ رﻮﻀﺣ رد ﯾﯿ
هرﺎﺼﻋ ﻦ
) mg/ml 16≤
( ﺪﻧدﻮﺑ ﺪﺷر ﻪﺑ ردﺎﻗ .
ﺖﻈﻠﻏ ﺎﻣا ﺎﻫ
ي ﺮﺗﻻﺎﺑ
ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ نﺎﻨﺷا هﺎ )
mg/ml 32
(≥ ﺪﺷر زا
ﻮﺳ ﯾﻪ ﺎﻫ ي ﭼرﺎﻗ ﯽ ﮔﻮﻠﺟ ﯿﺮ ي دﻮﻤﻧ .
Table 1 Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of Seidlitziarosmarinushydroalcoholic extract
MIC (mg/ml)
Extract concentration (mg/ml) MFC (mg/ml) Control
512 256 128 64
32 16
8 4 Fungi species
512 -
- - - - -
+ +
+ P. digitatum
> 512 -
- - - - -
+ +
+ P. italicum
+, grow; -, not grow.
ﺎﺘﻧ ﯾ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ نﻮﻣزآ ﺞ ﯽ
ﻪﮐ داد نﺎﺸﻧ ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ مﻮ
دﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻪﺑ ﺖﺒﺴﻧ ﻨﭘ
ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ﺖﻈﻠﻏ رﻮﻀﺣ رد
ﺮﺘﻤﮐ ي ﻏ هرﺎﺼﻋ زا ﯿ
ﻣ لﺎﻌﻓﺮ ﯽ ﺎﺘﺳار رد ﻪﮐ ددﺮﮔ ي
ﺎﺘﻧ ﯾ ﺞ
نﻮﻣزآ ﺎﻫ ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ ﻣ رﺎﮔآ ﮏﻫﺎﭼ و رﺎﮔآ نژﻮ ﯽ
ﺪﺷﺎﺑ .
ﻟﺎﻌﻓ ﯿ ﭼرﺎﻗﺪﺿ ﺖ ﯽ
ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ ﺖﺳا ﻦﮑﻤﻣ نﺎﻨﺷا هﺎ
ﺷﺎﻧ ﯽ ا زا ﯾ ﻘﺣ ﻦ ﯿ ﻫﺎﻣ ﺖﻠﻋ ﻪﺑ ﻪﮐ ﺪﺷﺎﺑ ﺖﻘ ﯿ
ﺮﮕﺑآ ﺖ ﯾ و ﺲﻧﺎﺳا ﺰ
هرﺎﺼﻋ ﺎﻫ ي ﻫ ﯿ ﻠﮑﻟاورﺪ ﯽ ا ، ﯾ ﮐﺮﺗ ﻦ ﯿ ز تﺎﺒ ﯾ ذﻮﻔﻧ ﻪﺑ ردﺎﻗ لﺎﻌﻓ ﺖﺴ
ﻣ رد ﯿ ﻠﺴ ﯿ ﮔﻮﻠﺟ و چرﺎﻗ مﻮ ﯿﺮ
ي ﻣ نآ ﺪﺷر زا ﯽ
ﺪﻨﺷﺎﺑ . ،ﻪﭼﺮﮔا
ﻧﺎﮑﻣ ﯿ ﻗد ﻢﺴ ﯿﻘ ﯽ ﭼرﺎﻗﺪﺿ ﺮﺛا ﺎﺑ طﺎﺒﺗرا رد ﯽ
نآ و دراﺪﻧ دﻮﺟو ﺎﻫ
اﯾ ﺪﻤﻋ ﺖﻟﺎﺣ ﻦ ﻟﺪﺑ ًﺎﺗ
ﯿ ﮐﺮﺗ رﻮﻀﺣ ﻞ ﯿ
ﺲﻧﺎﺳا رد ﻒﻠﺘﺨﻣ تﺎﺒ و ﺎﻫ
هرﺎﺼﻋ ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ . ا ﺎﺑ ﯾ ﻪﮐ ﺖﺳا هﺪﺷ شراﺰﮔ ،لﺎﺤﻨ
اﯾ ﮐﺮﺗ ﻦ ﯿ اﺰﻓا ﻪﺑ ردﺎﻗ تﺎﺒ ﯾ
ﺬﭘذﻮﻔﻧ ﺶ ﯾﺮ
ي ﺮﺨﺗ و ﯾ ،ءﺎﺸﻏ ﺐ
ژﻮﻟﻮﻓرﻮﻣ حﻼﺻا و چرﺎﻗ هزاﺪﻧا ﺶﻫﺎﮐ ي
ﻟﻮﻠﺳ ﯽ ﻣ ﯽ ﺪﻨﺷﺎﺑ ] 21 [ .
ﻤﮐ تﺎﻌﻟﺎﻄﻣ نﻮﻨﮐﺎﺗ ﯽ
ﮐﺮﺗ درﻮﻣ رد ﯿ
ﻓ تﺎﺒ ﯿ ﺷﻮﺘ ﯿﻤ ﯿﺎ ﯾ ﯽ ﻟﺎﻌﻓ ، ﯿ ﺖ
ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟﺎﻌﻓ و ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻫ هرﺎﺼﻋ ﯿ ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ هﺎ
ﺖﺳا ﻪﺘﻓﺮﮔ ترﻮﺻ نﺎﻨﺷا .
ﺰﻋ ﯾﺰ ﯾ ﺮﻫﺎﻃ و ﻪﻣﺮﺷ نﺎ ي
هداز
) 2014 ( ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ ﺪﻧدﻮﻤﻧ شراﺰﮔ ﯽ
ﮔ ﯿ وﺎﺣ نﺎﻨﺷا هﺎ ي
mg GAE/g 26
/7 و ﻞﮐ لﻮﻨﻓ mg QE/g
36 / 48 ﺋﻮﻧوﻼﻓ ﯿ ﺪ
ﻣ ﻞﮐ ﯽ ﻟﺎﻌﻓ و ﺪﺷﺎﺑ ﯿ
ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣ ترﺪﻗ سﺎﺳا ﺮﺑ نآ رﺎﻬ
دار ﯾ دازآ لﺎﮑ ﺎﺑ ﺮﺑاﺮﺑDPPH
63 / 73 ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺮﺘ
دﺮﮔ شراﺰﮔ ﯾ
ﺪ ] 25 [ . ﺰﻋ ﯾﺰ ﯾ نارﺎﮑﻤﻫ و ﻪﻣﺮﺷ نﺎ )
1396 ( ﺎﺑ
ﺳرﺮﺑ ﯽ ﻣ ﯿ ﺋﻮﻧوﻼﻓ ،ﻞﮐ لﻮﻨﻓ ناﺰ ﯿ
ﻟﺎﻌﻓ ،ﻞﮐ ﺪ ﯿ
ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ و
ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ هرﺎﺼﻋ ﺎﻫ ي ﺑآ ﯽ ﻟﻮﻧﺎﺘﻣ ، ﯽ ﻟﻮﻧﺎﺗا و ﯽ ﮔ ﯿ هﺎ
ﺑ نﺎﻨﺷا ﯿ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ ﻪﮐ ﺪﻧدﻮﻤﻧ نﺎ ﯽ
ﺑآ و ﯽ ﺗﺮﺗ ﻪﺑ ﯿ اراد ﺐ ي
ﺑﯿ ﺮﺘﺸ ﯾ ﺮﺘﻤﮐ و ﻦ ﯾ
دار رﺎﻬﻣ ترﺪﻗ ﻦ ﯾ
دازآ لﺎﮑ DPPH
) 150 100– ﻣ ﯿ ﻣ رد مﺮﮔوﺮﮑ ﯿﻠ
ﯽ ﻟﯿ ﺐﺴﺣ ﺮﺑ ﺮﺘ IC50
( اﻮﺘﺤﻣ ، ي
لﻮﻨﻓ ) mg GAE/g 25
- 15 ( اﻮﺘﺤﻣ ، ي ﺋﻮﻧوﻼﻓ ﯿ ﺪ
) mg QE/g 20
- 12 ( ﻟﺎﻌﻓ و ﯿ ﭼرﺎﻗ ﺪﺿ ﺖ ﯽ
ﻠﻋ ﯿ ژﺮﭙﺳآ ﻪ ﯾ سﻮﻠ
ﺎﻧﯾ ﺮﺠ ) 11 5– ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ﺪﻧﺎﮐ و ﯾ ﺒﻟآ اﺪ ﯿ ﺰﻨﮑ ) 15 10– ﻣ ﯿﻠ ﯽ ﺮﺘﻣ (
ﻣ ﯽ ﺪﻨﺷﺎﺑ ] 26 [ . ﻪﻌﻟﺎﻄﻣ رد ا ي د ﯾ ﺑاود ،ﺮﮕ ﯽ نارﺎﮑﻤﻫ و )
1399 (
ﺑﯿ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ ﺪﻨﺘﺷاد نﺎ ﯽ
اراد ي ﺑ ﯿ ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ﻟﺎﻌﻓ ناﺰ ﯿ ﺖ
ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺑآ هرﺎﺼﻋ و ﯽ
ﺮﺘﻤﮐ ﯾ ﻣ ﻦ ﯿ ﺘﻧآ ﺮﺛا ناﺰ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ار
ﻣ اراد ﯽ ا ﻪﮐ ﺪﺷﺎﺑ ﯾ ﺒﻄﻗ ﻪﺑ ﺖﻟﺎﺣ ﻦ ﯿ
بآ ﻪﺑ ﺖﺒﺴﻧ لﻮﻧﺎﺗا ﺮﺘﻤﮐ ﺖ
طﺎﺒﺗرا ﺮﻃ زا ﻪﮐ ﺪﺷ هداد ﯾ
ور ﺮﺑ ﺮﺛا ﻖ ي
د ﯾ ﻟﻮﻠﺳ هراﻮ ﯽ ﻢﻫ ﺮﺑ و
ﺎﻫرﺎﺘﺧﺎﺳ ندز ي
ﺘﻓﺎﺑ ﯽ ﺎﻧاﻮﺗ ﯾ ﯽ ﺑ ﯿ ﺮﺘﺸ ي ﮐﺮﺗ جاﺮﺨﺘﺳا رد ﯿ
تﺎﺒ
ﻟﻮﻨﻓ ﯽ ﺪﻧراد . ا ﺮﺑ هوﻼﻋ ﯾ
ﮐﺮﺗ رﻮﻀﺣ ،ﻦ ﯿ
ﺋﻮﻟﺎﮑﻟآ تﺎﺒ ﯿ
ﺪ ي ،
ﺋﻮﻧوﻼﻓ ﯿ ﺪ ي ﺋوﺮﺘﺳا ، ﯿ ﺪ ي ﻧﻮﭘﺎﺳ ، ﯿﻨ ﯽ ﻠﮔ ، ﯿ زﻮﮑ ﯾﺪ ي ﻨﻧﺎﺗ و ﯽ رد ﮔ هرﺎﺼﻋ
ﯿ ﺋﺎﺗ نﺎﻨﺷا هﺎ ﯿ
دﺮﮔ ﺪ ﯾ ﺪ ] 27 [ . توﺎﻔﺗ ﺎﻫ ي هﺪﺷ هﺪﻫﺎﺸﻣ
ﻣ رد ﯿ ﮐﺮﺗ ناﺰ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ / ﺋﻮﻧوﻼﻓ ﯿ ﺪ ي ﻟﺎﻌﻓ و ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ و
ﭼرﺎﻗ ﺪﺿ ﯽ ﮔ هرﺎﺼﻋ ﯿ
ا رﺪﻧﺎﻨﺷا هﺎ ﯾ
ﺎﻘﻣ رد ﻪﻌﻟﺎﻄﻣ ﻦ ﯾ
ﺎﺑ ﻪﺴ
ﯾ ﻪﺘﻓﺎ ﺎﻫ ي ﺎﺳ ﯾ ﻘﻘﺤﻣ ﺮ ﯿ شور ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ ﻦ ﺎﻫ
ي ﻒﻠﺘﺨﻣ
ﺖﻤﺴﻗ و جاﺮﺨﺘﺳا ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ اﺮﺑ هدﺎﻔﺘﺳا درﻮﻣ ي
جاﺮﺨﺘﺳا
ﺲﻧﺎﺳا / دﻮﺷ هداد ﺖﺒﺴﻧ هرﺎﺼﻋ .
ﺑ هوﻼﻋ ا ﺮ ﯾ غﻮﻠﺑ ،عﻮﻨﺗ ،ﺄﺸﻨﻣ ،ﻦ
ﮐ و ﺖﺷادﺮﺑ مﺎﮕﻨﻫ رد ﯿﻔ
ﯿ ﭘ رﺎﻨﮐ رد مﺎﺧ داﻮﻣ ﺖ ﯿ
ﺗ ﺶ ﯿ و ﺎﻫرﺎﻤ
شور ﺎﻫ ي ﮐﺮﺗ و دﺮﮑﻠﻤﻋ ،جاﺮﺨﺘﺳا ﯿ
ﺲﻧﺎﺳا ﺐ /
ﻞﺻﺎﺣ هرﺎﺼﻋ
ﺛﺄﺗ ﺖﺤﺗ ار ﯿ
ﻣ راﺮﻗ ﺮ ﯽ ﺪﻨﻫد ] 13 , 20 , , 28 , 29 [.
4 - ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي ﺎﻬﻧ ﯾ ﯽ
ﻫ هرﺎﺼﻋ ،ﻪﻌﻟﺎﻄﻣ ﻦﯾا ﺞﯾﺎﺘﻧ ﻖﺑﺎﻄﻣ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ رﻮﻄﺑ نﺎﻨﺷا هﺎ
ﺮﺛﺆﻣ ي دار رﺎﻬﻣ ﺐﺒﺳ ﯾ
لﺎﮑ ﺎﻫ ي دازآ وDPPH دﺮﮔABTS
ﯾ ﺪ
ﺷﺎﻧ ًﺎﺗﺪﻤﻋ ﻪﮐ ﯽ
ﮐﺮﺗ رﻮﻀﺣ زا ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ز ﯾ ﮔ رد لﺎﻌﻓ ﺖﺴ ﯿ
هﺎ
ﻣ نﺎﻨﺷا ﯽ ﺪﺷﺎﺑ . ا ﺮﺑ هوﻼﻋ ﯾ چرﺎﻗ ،ﻦ ﺎﻫ ي ﺳﻮﭘ و دﺎﺴﻓ ﻞﻣﺎﻋ ﯿ
ﮔﺪ ﯽ
لﺎﻘﺗﺮﭘ ﻨﭘ) ﯽ ﺳﯿ ﻠﯿ د مﻮ ﯾ ﺠ ﯿ مﻮﺗﺎﺘ ﻨﭘو ﯽ ﺳﯿ ﻠﯿ ا مﻮ ﯾ ﻟﺎﺘ ﯿ مﻮﮑ ( رﻮﻀﺣ رد
ﺖﻈﻠﻏ ﺎﻫ ي ﻻﺎﺑ ي ﻫ هرﺎﺼﻋ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﻗ نﺎﻨﺷا ﺪﺷر ﻪﺑ ردﺎ
ﺪﻧدﻮﺒﻧ . اﺮﺑﺎﻨﺑ ﯾ ﻫ هرﺎﺼﻋ ،ﻦ ﯿ
ﻠﮑﻟاورﺪ ﯽ ﮔ ﯿ ﻠﺑﺎﻗ نﺎﻨﺷا هﺎ ﯿ
هدﺎﻔﺘﺳا ﺖ
ﮐﺮﺗ ناﻮﻨﻌﺑ ﯿ
ﻣ ﺪﺿ ﺐ ﯿ
ﺘﻧآ و بوﺮﮑ ﯽ
ﺴﮐا ﯿ ﺒﻃ ناﺪ ﯿﻌ ﯽ ﺖﻬﺟ
ﮔﻮﻠﺟ ﯿﺮ ي ﺑ زا ﯿ رﺎﻤ ي ﺎﻫ ي ﻣ ﺖﺷادﺮﺑ زا ﺲﭘ ﯿ
هﻮ ﺰﺒﺳ و ﺎﻫ ﯾ و تﺎﺠ
ﺑ نﺎﻣرد ﯿ رﺎﻤ ي ﺎﻫ ي ﺷﺎﻧ ﯽ سﺮﺘﺳا زا ﺎﻫ
ي ﺴﮐا ﯿ ﺗاﺪ ﯿ اراد ار ﻮ
ﻣ ﯽ ﺪﺷﺎﺑ . ا ﺎﺑ ﯾ ﺎﺳﺎﻨﺷ ﺎﺑ ،لﺎﺤﻨ ﯾ
ﯽ ﮐﺮﺗ عﻮﻧ ﯿ هرﺎﺼﻋ رد هﺮﺛﺆﻣ تﺎﺒ
ﻫﯿ ﮑﻟاورﺪ ﯽ ﮔ ﯿ ﻣ نﺎﻨﺷا هﺎ ﯽ
ﻊﻣﺎﺟ تﺎﻌﻟﺎﻄﻣ ناﻮﺗ ﺮﺗ
ي طﺎﺒﺗرا رد
و ﻪﺑ ﯾ ﮔﮋ ﯽ ﺎﻫ ي ﺑ ﯿ ژﻮﻟﻮ ﯾ ﮑ ﯽ داد مﺎﺠﻧا نآ .
5 - ﺪﻘﺗ ﯾ ﺮﮑﺸﺗ و ﺮ
ﻣ مزﻻ دﻮﺧ ﺮﺑ ﻪﻟﺎﻘﻣ نﺎﮔﺪﻨﺴﯾﻮﻧ ﯽ
ﺸﻫوﮋﭘ ﺖﻧوﺎﻌﻣ زا ﺪﻨﻧاد ﯽ
و
روﺎﻨﻓ ي زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ
ﯽ ﻪﺑ نﺎﺘﺳزﻮﺧ
ﻟد ﯿ ﺎﻤﺣ ﻞ ﯾ ﺖ ﺎﻫ ي دﺎﻣ ي ﻮﻨﻌﻣ و ي ﻤﺻ ﯿ ﻧادرﺪﻗ و ﺮﮑﺸﺗ ﻪﻧﺎﻤ ﯽ
ﺪﻨﯾﺎﻤﻧ .
6 - ﻊﺑﺎﻨﻣ
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Inhibitory and lethality effect of Seidlitziarosmarinus hydroalcoholic extract on mold fungi causing orange fruit rot
(Penicillium digitatum and Penicillium italicum)
Rahmati-Joneidabad, M. 1*, Alizadeh Behbahani, B. 2, Noshad, M. 3
1. Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2. Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3. Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
ABSTRACT ARTICLE INFO
Article History:
Received 2022/ 02/ 07 Accepted 2022/ 04/ 04
Keywords:
Seidlitziarosmarinus,
Hydroalcoholic extract, Antifungal activity,
Antioxidant effect. In the present study, the hydroalcoholic extract of Seidlitziarosmarinus was
obtained using maceration method and its antifungal effect against fungi species causing orange fruit rot (Penicillium digitatum and Penicillium italicum) according to disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum fungicidal concentration were evaluated. In addition, the content of total phenols and flavonoids and the antioxidant activity of the hydroalcoholic extract of S. rosmarinus were determined. According to the results of disk diffusion and well diffusion agar tests, the antifungal activity of the extract was dependent on its concentration and P. digitatum was the most sensitive fungal strain to the extract. Moreover, both fungal strains were unable to grow in the presence of 32 mg/ml extract and the minimum fungicidal concentration for P.
digitatum was 512 mg/ml. The contents of total phenols and total flavonoids of the extract were 26.19 mg GAE/g and 11.10 mg QE/g, respectively. The results of antioxidant activity showed that the hydroalcoholic extract of S. rosmarinus is able to scavenge DPPH (112.14 μg/ml) and ABTS (103.46 μg/ml) free radicals. In general, the results of this study show that the hydroalcoholic extract of S. rosmarinus plant contains phenolic compounds with antifungal and antioxidant potential.
DOI: 10.52547/fsct.19.124.127
DOR: 20.1001.1.20088787.1401.19.124.30.7
*Corresponding Author E-Mail:
Iranian Journal of Food Science and Technology
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