ﺎﺳﺎﻨﺷ ﯾ ﯽ ﮐﺮﺗ ﯿ ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ
ﺳرﺮﺑ و زﺎ ﯽ
ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ
ﺑوﺮﮑ ﯽ ﺮﺑ نآ ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ،
ﻟﯿ ﺮﺘﺴ ﯾ ا ﺎ ﯾ اﻮﮐﻮﻨ ﺷﺮﺷا ، ﯿ ﻠﮐ ﺎ ﯽ ﺗ ﻼﻧﻮﻤﻟﺎﺳ ، ﯿﻔ
ﯽ ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ ، ﯾ
ازﻮﻨ ﺪﻧﺎﮐ و ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ
ﺸﺨﺑ ﺎﺿﺮﻣﻼﻏ ﯽ
ﻧﺎﺧ ﯿ ﮑ
1ﯽ ﺤﺻﺎﻧ داﺰﻬﺑ ،
2ﯽ ﻠﻋﺪﻤﺤﻣ ،*
ﯽ ﻫاﺮﺑا ﯿﻤ
3ﯽ ﺪﺣﻮﻣ اﺪﻧ ، ي
ﺮﺑﺎﺻ ﻠﻋ زوﺮﻬﺑ ،4
ﯿ ﻧﺎﻬﺒﻬﺑ هداﺰ
5ﯽ
1 - ﺑ هوﺮﮔ ،دﺎﺘﺳا ﯿ
ژﻮﻟﻮﻨﮑﺗﻮ ي زروﺎﺸﮐ ي ﭘ هﺎﮕﺸﻧاد ، ﯿ
ا ،قﺮﺷ ناﺮﻬﺗ ﺪﺣاو رﻮﻧ مﺎ ﯾ
ناﺮ .
2 - ﺸﻧاد ﯿ ﺳﺪﻨﻬﻣ هوﺮﮔ رﺎ ﯽ
روﺎﻨﻓ و ي زروﺎﺸﮐ ي ﭘ هﺎﮕﺸﻧاد ، ﯿ
ا ،رﻮﻧ مﺎ ﯾ ناﺮ .
3 - ﺑ هوﺮﮔ ،دﺎﺘﺳا ﯿ
ژﻮﻟﻮﻨﮑﺗﻮ ي زروﺎﺸﮐ ي ﭘ هﺎﮕﺸﻧاد ، ﯿ
ا ،قﺮﺷ ناﺮﻬﺗ ﺪﺣاو ،رﻮﻧ مﺎ ﯾ
ناﺮ .
4 - ز ﻪﺘﺷر ﻪﺘﺧﻮﻣآ ﺶﻧاد ﯾ
روﺎﻨﻓ ﺖﺴ ي ﭘ هﺎﮕﺸﻧاد،
ﯿ ا ،قﺮﺷ ناﺮﻬﺗ ﺪﺣاو ،رﻮﻧ مﺎ ﯾ
ناﺮ .
5 - دﺎﺘﺳا ﯾ ﺳﺪﻨﻬﻣ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯽ
ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﻣاد مﻮﻠﻋ هﺪﮑﺸﻧاد ، ﯽ
ﺎﻨﺻ و ﯾ اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ي
ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ
ﯽ
ﻧﺎﺛﻼﻣ ،نﺎﺘﺳزﻮﺧ ﯽ
ا ، ﯾ ناﺮ .
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 25 / 10 / 1400
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 24 / 11 / 1400
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
ﭘ ﺲﻧﺎﺳا ﯿ زﺎ
،
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
،
ﺖﻗر زﺎﺳ ي ﮏﻫﺎﭼ رد
،
ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ ﯽ
زﺎﮔ ي .
DOI: 10.52547/fsct.19.123.225
DOR: 20.1001.1.20088787.1401.19.123.30.5
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ : [email protected]
نﺎﯾز ﺶﻫﺎﮐ ﺖﻬﺟ يرﺎﻤﯿﺑ ﻞﻣاﻮﻋ زا ﯽﺷﺎﻧ ﯽﻧﺎﺟ تاﺮﻄﺧ و يدﺎﺼﺘﻗا يﺎﻫ
زا هدﺎﻔﺘﺳا ،ﯽﺑوﺮﮑﯿﻣ ياز
يﺮﺘﮐﺎﺑ رﻮﻀﺣ لﺮﺘﻨﮐ ياﺮﺑ ﺮﺛﻮﻣ ﯽﺷور ﯽﺑوﺮﮑﯿﻣﺪﺿ تﺎﺒﯿﮐﺮﺗ ناﻮﻨﻋ ﻪﺑ ﯽﻌﯿﺒﻃ داﻮﻣ يرﺎﻤﯿﺑ يﺎﻫ
ﻪﺑ از
ﯽﻣ ﺮﻈﻧ ﺪﺳر . ا رد ﯾ ﮐﺮﺗ اﺪﺘﺑا ،ﺶﻫوﮋﭘ ﻦ ﯿ
ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ هﺎﮕﺘﺳد ﺎﺑ زﺎ ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ
ﯽ زﺎﮔ ي
ﻃ ﻪﺑ ﻞﺼﺘﻣ ﯿ
ﻒ ﻣﺮﺟ ﺞﻨﺳ ﯽ ﻌﺗ ﯿﯿ دﺮﮔ ﻦ ﯾ ﺪ . شور زا ﺎﻫ ﯾ رﺎﮔآ رد رﺎﺸﺘﻧﺎ ﺳرﺮﺑ ﺖﻬﺟ رﺎﮔآ ﮏﻫﺎﭼ و
ﯽ ﺮﻄﻗ
ﺖﻗر شور،ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ زﺎﺳ
ي ﮏﻫﺎﭼ رد )ﻣ
ﯿ اد ثاﺮﺑوﺮﮑ ﯾ
ﻦﺷﻮﻠ ( ﮔزاﺪﻧا ﺖﻬﺟ ﯿﺮ
ي ﺖﻈﻠﻏ ﻞﻗاﺪﺣ
ﻨﭽﻤﻫ و ﺪﺷر هﺪﻨﻨﮐرﺎﻬﻣ ﯿ
ﻠﭘرﻮﭘ شور زﺎﻨ ﯿ
اﺮﺑ ﺖ ي ﺪﺷ هدﺎﻔﺘﺳا ﯽﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ .
عﻮﻤﺠﻣ رد 20
ﯽﯾﺎﯿﻤﯿﺷ راّﺮﻓ هدﺎﻣ )
ﺮﻘﺗ ﯾ
ًﺎﺒ 100 ﮐﺮﺗ ﺪﺻرد ﯿ
ﭘ ﺲﻧﺎﺳا تﺎﺒ ﯿ
زﺎ ( ﺎﺳﺎﻨﺷ ﯾ ﯽ ﺪﺷ . ﮐﺮﺗ ﯿ تﺎﺒ Trisulfide
dipropyl ،
Disulfide dipropyl
، n-Decane و
Furfuryl methyl sulfide ﺗﺮﺗ ﻪﺑ
ﯿ ﺎﺑ ﺐ 67 / 20 ،
18 / 16 ، 51 / 13 و 23 / 10 اﺰﺟا ﺪﺻرد ي
ﻠﺻا ﯽ ﮑﺸﺗ ﯿ ﭘ ﺲﻧﺎﺳا هﺪﻨﻫد ﻞ ﯿ
ﺪﻧدﻮﺑ زﺎ . ﺎﺘﻧ ﯾ ﮏﻫﺎﭼ نﻮﻣزآ ﺞ
ﭘ ﺲﻧﺎﺳا ﻪﮐ داد نﺎﺸﻧ رﺎﮔآ ﯿ
ﺑ زﺎ ﯿ ﺮﺘﺸ ﯾ ﻢﮐ و ﻦ ﺮﺗ ﯾ ﺛﺎﺗ ﻦ ﯿ ﺗﺮﺗ ﻪﺑ ار ﺮ ﯿ
ﺮﺑ ﺐ ﺮﺘﮐﺎﺑ ي ﺖﺒﺜﻣ مﺮﮔ
ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﺮﻄﻗ ﺎﺑ
15 ﻣ ﯿﻠ ﯽ ﺮﺘﮐﺎﺑ و ﺮﺘﻣ ي
ﻔﻨﻣ مﺮﮔ ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳﯽ
ﯾ ازﻮﻨ ﺮﻄﻗ ﺎﺑ 10
ﻣﯿ ﻠ ﯽ ﺖﺷاد ﺮﺘﻣ .
ﺎﺘﻧ ﯾ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ نﻮﻣزآ ﺞ ﯽ
ﭘ ﺲﻧﺎﺳا ﺪﺷر زا ﯿ
اﺮﺑ زﺎ ﻓﺎﺘﺳاي ﯿ سﻮﮐﻮﮐﻮﻠ
سﻮﺋروا ﻟﯿ ، ﺮﺘﺴ ﯾ ا ﺎ ﯾ اﻮﮐﻮﻨ ﺷﺮﺷا ، ﯿ ﻠﮐ ﺎ ﯽ ﺗ ﻼﻧﻮﻤﻟﺎﺳ ، ﯿﻔ
ﯽ ﺋا سﺎﻧﻮﻣودﻮﺳ ، ژوﺮ
ﯾ ازﻮﻨ ﺪﻧﺎﮐ و ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ ﻪﺑ
ﺗﺮﺗ ﯿ ﺐ 512 ، 512 ، 512 ، 512 ، 512 و 1024 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ دﻮﺑ ﺮﺘ . ﻠﮐ رﻮﻃ ﻪﺑ ﯽ
ﺎﺘﻧ ﯾ زا ﻞﺻﺎﺣ ﺞ
اﯾ ﻣﺪﺿ ﺮﺛا ﻪﮐ داد نﺎﺸﻧ ﺶﻫوﮋﭘ ﻦ ﯿ
ﺑوﺮﮑ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ دوﺪﺣ ﺎﺗ زﺎ ي
ﻣ عﻮﻧ ﻪﺑ ﻪﺘﺴﺑ ﯿ
ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ
ﻣ ﯽ توﺎﻔﺘﻣ ﺪﻧاﻮﺗ ﺪﺷﺎﺑ
.
ﻪﻠﺠﻣ
مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ
ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ
1 - ﻪﻣﺪﻘﻣ
ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﻧﻮﻔﻋ ﯽ ناﻮﻨﻋ ﻪﺑ ﯾ
ﺪﻬﺗ ﮏ ﯾ اﺮﺑ ﻢﻬﻣ ﺪ ي
ﺖﻣﻼﺳ
ﻧﺎﺴﻧا ﻊﻣاﻮﺟ ﯽ
ﻣ رﺎﻤﺷ ﻪﺑ ﯽ
ﺪﻧور . ﺘﻧآ ﻒﺸﮐ ﺎﺑ ﯽ
ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﺞ
ﻧﺎﻣرد ﯽ ﺷﺎﻧ گﺮﻣ ، ﯽ
ﺑ زا ﯿ رﺎﻤ ي ﺎﻫ ي ﻧﻮﻔﻋ ﯽ ﻞﺑﺎﻗ رﻮﻃ ﻪﺑ
ﻪﻈﺣﻼﻣ ا ي ﺶﻫﺎﮐ ﯾ
،ﺖﻓﺎ ﺎﻣا ﺑ هدﺎﻔﺘﺳا ﻪﻧﺎﻔﺳﺎﺘﻣ ﯽ
ور ﯾ و ﻪ
ﻏﯿ ﻟﻮﺻاﺮ ﯽ ﺘﻧآ زا ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﻧﺎﻣرد ﺞ ﯽ ﺷﺎﻧ ﺖﻣوﺎﻘﻣ و ﯽ
زا
نآ ا ﺖﺸﮔزﺎﺑ ﺚﻋﺎﺑ ﺎﻫ ﯾ
ﺑ ﻦ ﯿ رﺎﻤ ي ﺖﺳا هﺪﺷ ﺎﻫ .
ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
اﺰﻓا ﯾ ﻣ ﺖﻣوﺎﻘﻣ ﺶ ﯿ
ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي ﺮﺑاﺮﺑ رد از
ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﻧﺎﻣرد ﺞ ﯽ ﻧ نﺎﻬﺟ ، ﯿ ﻣﺮﺒﻣ زﺎ ﯽ ﻐﺗ ﻪﺑ ﯿﯿ ﻮﮕﻟا ﺮ ي
فﺮﺼﻣ ﺪﺟ ﻊﺑﺎﻨﻣ ﻒﺸﮐ و ﯾ
ﺮﺘﻤﮐ ﺎﺑ ﺪ ﯾ ﺒﻧﺎﺟ ﺮﺛا ﻦ ﯽ
اﺮﺑ ي ﺖﻣﻼﺳ
نﺎﺴﻧا دراد ﺎﻫ ] 1 و 2 [.
ﺤﺻ نﺎﻣرد ﯿ
ﺘﻧآ ﺎﺑ ﺖﺳرد و ﻊﻗﻮﻣ ﻪﺑ ،ﺢ ﯽ
ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﺞ
ﻧﺎﻣرد ﯽ ﻤﻬﻣ مﺎﮔ ﯽ ﺑ دﻮﺒﻬﺑ رد ﯿ
رﺎﻤ ي ﺎﻫ ي ﻧﻮﻔﻋ ﯽ بﺎﺴﺣ ﻪﺑ
ﻣ ﯽ آﯾ ﺪ.
ﭘﯿ اﺪ ﯾ ﺮﺘﮐﺎﺑ رد ﺖﻣوﺎﻘﻣ ﺶ ي
ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي ﻪﺑ ﺖﺒﺴﻧ از
ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﻧﺎﻣرد ﺞ ﯿﯿ ﮑ ﯽ ﻧﺎﻣرد تﻼﮑﺸﻣ زا ﯽ
نﺎﻬﺟ رد
ﻣ ﯽ ﺪﺷﺎﺑ .ا ﯾ ﻣ ﻦ و ﻪﺑ ﻪﻠﺌﺴ ﯾ ﺎﻫرﻮﺸﮐ رد هﮋ ﯾ
ﯽ نآ رد ﻪﮐ فﺮﺼﻣ ﺎﻫ
ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﻮﺠﺗ نوﺪﺑ ﮏ ﯾ
ﻏو ﮏﺷﺰﭘ ﺰ ﯿ
ﻟﻮﺻاﺮ ﯽ ﺑ ﺖﺳا ﯿ ﻞﺑﺎﻗ ﺮﺘﺸ
ﻣ ﻪﺟﻮﺗ ﯽ ﺪﺷﺎﺑ . ﺶﻫوﮋﭘ ﺎﻫ ي ﺴﺑ ﯿ رﺎ ي ﺬﭘ مﺎﺠﻧا نﺎﻬﺟ رد ﯾ
ﻪﺘﻓﺮ
ﺎﺘﻧ ﻪﮐ ﺖﺳا ﯾ
ا ﺞ ﯾ ﻪﻌﻟﺎﻄﻣ ﻦ ﺎﻫ ﻮﻣ ﯾ ﺖﻣوﺎﻘﻣ ﺪﻧور ﻪﮐ ﺖﺳا نآ ﺪ
ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮑ ﯽ ﻧد طﺎﻘﻧ ﺮﺜﮐا رد ﯿ
ﺪﺟ ترﻮﺻ ﻪﺑ ﺎ ي
اﺰﻓا ﯾ ﺶ ﯾ ﺘﻓﺎ ﻪ
ﺖﺳا ] 3 و 4[
. ﺶﻫوﮋﭘ ﻖﺒﻃ ﺎﻫ
ي ﺧا ﯿﺮ اﺰﻓا ﯾ نوﺰﻓازور ﺶ
وراد ﺖﻣوﺎﻘﻣ ﯾ
ﯽ ﻪﺑ ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﻧﺎﻣرد ﺞ ﯽ ﺳﺎﺴﺣ و ﯿ ﺖ
ﮐﺮﺗ ﻪﺑ ﯿ ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾ ﯽ ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﻻد ﻪﻠﻤﺟ زا ﯾ
ور ﻞ ﯾ دﺮﮑ
ﻘﻘﺤﻣ ﯿ اﺮﺑ ﻦ ي ﯾ ﮐﺮﺗ ﻦﺘﻓﺎ ﯿ ﻣﺪﺿ تﺎﺒ ﯿ
ﺑوﺮﮑ ﯽ ﮔ ﺄﺸﻨﻣ ﺎﺑ ﯿ
ﻫﺎ ﯽ و
اراد ي ﺒﻧﺎﺟ ﺮﺛا ﯽ اﺮﺑ ﻢﮐ ي نﺎﺴﻧا ﺖﻣﻼﺳ ﻣ ﺎﻫ
ﯽ ﺪﺷﺎﺑ . ا زا ﯾ ﻦ ور
ﮐﺮﺗ ﯿ ﻣﺪﺿ تﺎﺒ ﯿ
ﺑوﺮﮑ ﯽ ﺎﭘ ﺮﺑ ﯾ ﮔ ﻪ ﯿ ﻫﺎ ﯽ ﻣ ﯽ لﺮﺘﻨﮐ رد ﺪﻨﻧاﻮﺗ
ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﻧﺎﺴﻧا ﯽ ﺷزرا ﺎﺑ ﺶﻘﻧ ﯽ
ا ﯾ ﺎﻤﻧ ﺎﻔ ﯾ ﺪﻨ ]5 و 6 [.
ﻌﺑﺎﻨﻣ ﯽﯾوراد نﺎﻫﺎﯿﮔ ﯽ
ﺖﯿﻟﻮﺑﺎﺘﻣ زا ﯽﻨﻏ ﻊﻗاو رد و ﻪـﯾﻮﻧﺎﺛ يﺎـﻫ
ﺎﯾ ﮏﯾ ﻪﮐ هدﻮﺑ ﯽـﯾوراد داﻮﻣ زا يرﺎﯿﺴﺑ ﯽﺳﺎﺳا هﺮﺛﺆﻣ ﻊﺑﺎﻨﻣ ماﺪﻧا زا ﯽﺧﺮﺑ ﻫ
نآ يﺎ هدﺎﻣ يوﺎﺣ ﺎﻫ ﺳﺎﺳا ي
ﯽ ﺖـﺳا . هدﺎﻣ ﻦﯾا
ﻢﮐ ﻻﻮﻤﻌﻣ ﻪﮐ زا ﺮﺗ
1 ﯽﻣ ﻞﯿﮑﺸﺗ ار هﺎﯿﮔ ﮏﺸﺧ نزوﺪﺻرد ،ﺪﻫد
اﻮﻓ ياراد ﯾ ﺖـﺳا هﺪـﻧز تادﻮـﺟﻮﻣ ﺮﺑ ﺐﺳﺎﻨﻣ ﯽﯾوراد ﺪ .
نﺎﻣز
ﻗد ﯿ ﮔ زا هدﺎﻔﺘﺳا ﻖ ﯿ
وراد نﺎﻫﺎ ﯾ ﯽ نﺎﺴﻧا ﻂﺳﻮﺗ وراد ناﻮﻨﻋ ﻪﺑ ﺎﻫ
ﻟو ﺖﺴﯿﻧ مﻮـﻠﻌﻣ ﯽ
اﻮﻓ ﻪـﺑ طﻮـﺑﺮﻣ تﺎـﻋﻼﻃا ﯾ
اﻮﺧ و ﺪ ص
نﺎﻣز زا ﯽﯾوراد نﺎﻫﺎﯿﮔ و هﺪـﻣآ ﺖﺳد ﻪﺑ رود رﺎﯿﺴﺑ يﺎﻫ
ﻞﺴﻧ رﺎﯿﺘﺧا رد مﺎﺠﻧاﺮﺳ ﺖـﺳا ﻪـﺘﻓﺮﮔ راﺮﻗ ﺮﺻﺎﻌﻣ يﺎﻫ
] 7 [.
ﭘﯿ ﺎﺑ زﺎ ﻤﻠﻋ مﺎﻧ
ﯽ Allium cepa ﮔﯿ
ﻫﺎ ﯽ
زا هدﺮﺳ ﺳ ﯿﺮ رد زﯾ ﺗﺮ ﯿ هﺮ ﭘﯿ زﺎ ﯾ نﺎ زا ﺗﯿ هﺮ ﺴﮔﺮﻧ ﯿ نﺎ ﺖﺳا ﭘ ﯿ. زا زﺎ هداﻮﻧﺎﺧ
ﻟآﯿ
1ﻪﺳﺎ ﻣ ﯽ ﮔ ﺮﻈﻧ زا و ﺪﺷﺎﺑ ﯿ
هﺎ ﺳﺎﻨﺷ ﯽ ﮔ ﯿ ﻫﺎ ﯽ ود ﺖﺳا
ﻪﺗﻮﺑ ﺪﺷر ﺎﺑ ﻪﻟﺎﺳ ا
ي ﻌﺿ ﯿ ﻒ ﺖﺳا . ﻪﺑ ناﺮﯾا تﻼﻓ ﯽﺸﺨﺑ ناﻮﻨﻋ
ﺖﺳا ﯽﮐارﻮﺧ زﺎﯿﭘ نﺪﺷ ﯽﻠﻫا و عﻮﻨﺗ ﺰﮐﺮﻣ ،يﺰﮐﺮﻣ يﺎﯿﺳآ زا .
ﭘﯿ ﮐﺮﺗ ﺰﻈﻧ زا زﺎ ﯿ
ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾ ﯽ وﺎﺣ ي ﺋﻮﻧﻼﻓ ﯿ ﮑﻟآ و ﺎﻫﺪ ﯿ
ﻞ
ﺳ ﯿ ﺌﺘﺴ ﯿ ﺴﮐﻮﻔﻟﻮﺳ ﻦ ﯿ
ا ﻪﮐ هدﻮﺑ دﺎ ﯾ
ﮐﺮﺗ ﻦ ﯿ ﻤﻫا تﺎﺒ ﯿ ﺖ و ﯾ هﮋ ا ي رد
ﺬﻐﺗ ﯾ ﺪﻧراد ﺖﻣﻼﺳ و ﻪ .
ﮐﺮﺗ ﯿ ﮑﻟآ ﺐ ﯿ ﺳ ﻞ ﯿ ﺌﺘﺴ ﯿ ﺴﮐﻮﻔﻟﻮﺳ ﻦ ﯿ
زا دﺎ
ﻢﻬﻣ ﺮﺗ ﯾ ﭘ ﻦ ﯿ ﺶ ﻢﻌﻃ داﻮﻣ زﺎﺳ ﺖﺳا راد
. ﭘﯿ اراد زﺎ ي ﮐﺮﺗ ﯿ تﺎﺒ
دﺮﮔﻮﮔ ي ﺴﻠﮐ ،ﺪﻨﻗ ، ﯿ
ﺪﺳ حﻼﻣا ،ﻢ ﯾ
ﺳﺎﺘﭘ و ﻢ ﯿ ،ﻢ ﯾ ﺳ ،ﺪ ﯿﻠ ﯿ ،ﺲ
ﻔﻠﺘﺨﻣ عاﻮﻧا و ﺮﻔﺴﻓ ،ﻦﻫآ ﯽ
و زا ﯾ ﻣﺎﺘ ﯿ ﻦ ﺎﻫ ي ) ،A و B (C
ﻣ ﯽ ﺪﺷﺎﺑ . ﭘ ﺲﻧﺎﺳا ﯿ ﺑ زا ﻦﺘﺨﭘ ﺮﺛا رد زﺎ ﯿ
ﻣ ﻦ ﯽ دور . ﭘﯿ مﺎﺧ زﺎ
ﻟﺎﻌﻓ ﯿ ﺪﺸﺗ ار هﺪﻌﻣ ﺖ ﯾ
ﻣ ﺪ ﯽ ﺚﻋﺎﺑ و ﺪﻨﮐ اﺰﻓا
ﯾ ﺢﺷﺮﺗ ﺶ
ﺳا ﯿ ﺮﻠﮐﺪ ﯾ رﺪ ﯾ ﻣ ﮏ ﯽ دﻮﺷ . ﮐﺮﺗ ﯿ ﭘ رد دﻮﺟﻮﻣ تﺎﺒ ﯿ
وﺎﺣ زﺎ ي تاﺮﺛا
ﺘﻧآ ،نﻮﺧ نﺪﺷ ﻪﺘﺨﻟ ﺪﺿ ،نﺎﻃﺮﺳ ﺪﺿ ﯽ
ﻫﯿ ﻣﺎﺘﺴ ﯿﻨ ﯽ و
ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ ]8 و 9 .[
ا زا فﺪﻫ ﯾ ﺎﺳﺎﻨﺷ ،ﺶﻫوﮋﭘ ﻦ ﯾ
ﯽ ﮐﺮﺗ ﯿ ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾ ﯽ ﺳا ﺲﻧﺎ ﭘﯿ و زﺎ
زرا ﯾﺑﺎ ﯽ ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﺮﺑ نآ ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ،
ﺷﺮﺷا ﯿ ﻠﮐ ﺎ ﯽ ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ ، ﯾ
ازﻮﻨ ﺗ ﻼﻧﻮﻤﻟﺎﺳ ، ﯿﻔ
ﯽ
،
ﻟﯿ ﺮﺘﺴ ﯾاﺎ ﯾ اﻮﮐﻮﻨ ﺪﻧﺎﮐ و ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ اﺮﺷ رد ﯾ نوﺮﺑ ﻂ ﻨﺗ ﯽ دﻮﺑ .
2 - شور و داﻮﻣ ﺎﻫ
2 - 1 - ﺷ داﻮﻣ ﯿﻤ
ﯿﺎ ﯾ ﯽ ﺤﻣ و ﯿ ﻂ ﺎﻫ ¬ ي ﺖﺸﮐ
ﻣﯿ ﺑوﺮﮑ ﯽ
ﺮﺗ فﺮﻌﻣ ي ﻞﻨﻓ ﻟوزاﺮﺘﺗ ﯿ مﻮ اﺮﻠﮐ ﯾ ﺪ ) ﺳﯿ رﺪﻟآ ﺎﻤﮕ ﯾ ﺮﻣآ ،ﭻ ﯾ ﺎﮑ ( ،
دﯾ ﮏﺴ ﺎﻫ ي ﺐﻃ ﻦﺗدﺎﭘ ﮏﻧﻼﺑ )اﯾ
ناﺮ ( ﺤﻣ و ﯿ ﻂ ﺎﻫ ي ﺮﻟﻮﻣ ﺖﺸﮐ
ﻫﯿ ﻫ ﺮﻟﻮﻣ ،رﺎﮔآ نﻮﺘﻨ ﯿ
زوﺮﺑﺎﺳ ،رﺎﮔآ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ ،ثاﺮﺑ نﻮﺘﻨ
ثاﺮﺑ زوﺮﺘﺴﮐد )
نﺎﻤﻟآ ،كﺮﻣ (
ﻬﺗ ﯿ ﺪﻧﺪﺷ ﻪ .
2 - 2 - ﺎﺳﺎﻨﺷ ﯾ ﯽ ﮐﺮﺗ ﯿ ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾﯿ ﭘ ﺲﻧﺎﺳﺎ ﯿ
ﺎﺑ زﺎ
ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ هﺎﮕﺘﺳد ﯽ
زﺎﮔ ي ﻪﺑ ﻞﺼﺘﻣ
ﻃﯿ ﻒ ﻣﺮﺟ ﺞﻨﺳ ﯽ
رﺰﺗ زا ﯾ ﻖ 2 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﭘ ﺲﻧﺎﺳا ﺮﺘ ﯿ
ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ هﺎﮕﺘﺳد ﻪﺑ زﺎ ﯽ
زﺎﮔ ي (Agilent Technologies 7890 A) ﻧﻮﺘﺳ ﺎﺑ
ﯽ ﻪﺑ
لﻮﻃ 30 ﻠﺧاد ﺮﻄﻗ ،ﺮﺘﻣ ﯽ
25 /0 ﻣ ﯿﻠ ﯽ ﻻ ﺖﻣﺎﺨﺿ و ﺮﺘﻣ ﯾ
ﻠﺧاد ﻪ ﯽ
25 /0 ﻣ ﯿ ﺮﺘﻣوﺮﮑ ) Agilent Technologies Inc.,HP-5
(MS ﻃ ﻪﺑ ﻞﺼﺘﻣ ﯿ
ﻣﺮﺟ رﺎﮕﻧ ﻒ ﯽ
(Agilent
Technologies 5975) ﺎﺳﺎﻨﺷ ﺖﻬﺟ
ﯾ ﯽ ﮐﺮﺗ ﯿ ﺲﻧﺎﺳا تﺎﺒ
ﭘﯿ ﺪﺷ هدﺎﻔﺘﺳا زﺎ ]
10 [ .
2 - 3 - ﻬﺗ ﯿﻪ ﺴﻧﺎﭙﺳﻮﺳ ﯿ
ﻣ نﻮ ﯿ ﺑوﺮﮑ ﯽ شور و ﺎﻫ
ي
زرا ﯾﺑﺎ ﯽ ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ
ﺑوﺮﮑ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ
زﺎ
اﺮﺑ ي ﻬﺗ ﯿ ﺴﻧﺎﭙﺳﻮﺳ ﻪ ﯿ
ﻣ نﻮ ﯿ ﺑوﺮﮑ ﯽ ﻮﺳ هزﺎﺗ ﺖﺸﮐ زا ﯾﻪ
ﺎﻫ ي
ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ) Staphylococcus aureusATCC 25923
،
Pseudomonas aeruginosaPTCC
،1707
Escherichia
coliATCC 25922
، Listeria innocuaATTC
33090 و Salmonella typhi PTCC 1609 ﭼرﺎﻗ و
ﯽ
) Candida albicans PTCC5027 (
ﺪﺷ هدﺎﻔﺘﺳا .
اﺮﺑ ي ﻬﺗ ﯿ ﻪ
ﺴﻧﺎﭙﺳﻮﺳ ﯿ ﺎﺑ نﻮ ﺮﺘﮐ ﯾﺎﯾ ﯽ ﭼرﺎﻗ و ﯽ ﺗﺮﺗ ﻪﺑ ﯿ ﺐ 24 و 72 ﺖﻋﺎﺳ
نﻮﻣزآ مﺎﺠﻧا زا ﻞﺒﻗ ﺎﻫ
ي ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ
، زا ﺧذ ﺖﺸﮐ ﯿ هﺮ ﻪﺑ ﺤﻣ ﯿ ﻂ
ﺖﺸﮐ ﻫ ﺮﻟﻮﻣ ﯿ رﺎﮔآ نﻮﺘﻨ زوﺮﺑﺎﺳ و
رﺎﮔآ زوﺮﺘﺴﮐد ﻘﻠﺗ
ﯿ ﺢ رد هﺪﺷ
ﺎﻫﺎﻣد ي 37 و 25 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
دﺮﮔ ﻪﺑﻮﮑﻧا داﺮﮔ ﯾﺪ
. زا ﺲﭘ
مﺮﮔ نﺎﻣز ﺖﺷﺬﮔ ﻪﻧﺎﺧ
راﺬﮔ ي ﺤﻣ ﺢﻄﺳ ﯿ
ﺖﺸﮐ ﻂ ﺎﺑ
لﻮﻠﺤﻣ
رﯾ ﺮﺘﺳا ﺮﮕﻨ ﯾ ﻞ ﻪﺘﺴﺷ هﺪﺷ و ﺴﻧﺎﭙﺳﻮﺳ ﯿ ﻣ نﻮ ﯿ ﺑوﺮﮑ ﯽ ﺎﺑ لﻮﻠﺤﻣ
رﯾ ﻗر ﺮﮕﻨ ﯿ ﻖ دﺮﮔ ﯾﺪ ﺎﺗ ﻧﺎﻣز ﯽ ﻣ ﻪﮐ ﯿ ناﺰ بﺬﺟ ﺴﻧﺎﭙﺳﻮﺳ ﯿ نﻮ رد
جﻮﻣ لﻮﻃ 620
ﺮﺑاﺮﺑ ﺎﺑ ﻣ ﯿ ناﺰ بﺬﺟ دراﺪﻧﺎﺘﺳا لﻮﻠﺤﻣ 5/
0
ﮏﻣ ددﺮﮔ ﺪﻨﻟرﺎﻓ ]
11 و 12 [.
شور زا ﺎﻫ ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ ،رﺎﮔآ نژﻮ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ﯽ
ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و
ﮔﺪﻨﺸﮐ ﯽ ﺳرﺮﺑ رﻮﻈﻨﻣ ﻪﺑ ﯽ
ﻣ ﺪﺿ ﺮﺛا ﯿ ﺑوﺮﮑ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ هدﺎﻔﺘﺳا زﺎ
ﺪﺷ .
2 - 4 - د ﯾ د ﮏﺴ ﯾﻔ ﯿ رﺎﮔآ نژﻮ
اﺬﻏ نﺎﻣزﺎﺳ و
د شور وراد ﯾ
د ﮏﺴ ﯾﻔ ﯿ ناﻮﻨﻋ ﻪﺑ ار رﺎﮔآ نژﻮ
ﺷور ﯽ اﺮﺑ دراﺪﻧﺎﺘﺳا ي
ﺎﻣزآ ﯾ هﺎﮕﺸ ﺎﻫ ي ﺨﺸﺗ ﯿ ﻟﺎﺑ ﺺ ﯿﻨ ﯽ ﺖﺒﺛ
ﺖﺳا هدﻮﻤﻧ .
د رد رﺎﺸﺘﻧا شور ﯾ
ز لﺎﻤﺘﺣا ﻪﺑ ﮏﺴ ﯾ
دﺎ
ﺮﺗدﺮﺑرﺎﮐﺮﭘ ﯾ
ﻟﺎﻌﻓ ﺶﺠﻨﺳ شور ﻦ ﯿ
ﺪﺿ ﺖ ﻣﯿ ﺑوﺮﮑ ﯽ رد
ﻪﻌﻟﺎﻄﻣ ﺎﻫ ي ﺳرﺮﺑ ﯽ هرﺎﺼﻋ و ﺲﻧﺎﺳا ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ . رد
اﯾ ﺮﺘﭘ اﺪﺘﺑا شور ﻦ ي
دﯾ ﺎﺑ ﺶ 20 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺤﻣ ﺮﺘ ﯿ ﺖﺸﮐ ﻂ
ﺮﺘﺳا ﯾ ﻫ ﺮﻟﻮﻤﻠ ﯿ رﺎﮔآ نﻮﺘﻨ )
ﻮﺳ ﯾﻪ ﺎﻫ ﯾ ﯽ ﺮﺘﮐﺎﺑ ي ( زوﺮﺑﺎﺳ و
رﺎﮔآ زوﺮﺘﺴﮐد )
ﻮﺳ ﯾ چرﺎﻗ ﻪ ( ﺪﺷ ﺮﭘ . ﻮﺳ ﯾﻪ ﺎﻫ ﯾﺒﯿ رﺎﻤ ي از ي درﻮﻣ
ﺳرﺮﺑ ﯽ د ا ر ﯾ ﺖﻈﻠﻏ رد ﺶﻫوﮋﭘ ﻦ ﻧ لدﺎﻌﻣ ﻪﮐ دراﺪﻧﺎﺘﺳا
ﯿ ﻢ
ﮏﻣ ﻨﻤﭼ ترﻮﺻ ﻪﺑ دﻮﺑ ﺪﻨﻟرﺎﻓ ﯽ
ﺤﻣ ﺢﻄﺳ ﺮﺑ ﯿ
ﻂ ﺎﻫ ي ﺖﺸﮐ
ﻣﯿ ﺑوﺮﮑ ﯽ دﺮﮔ ﺶﺨﭘ ﯾﺪ
. دﯾ ﮏﺴ ﺎﻫ ي ﺬﻏﺎﮐ ي وﺎﺣ ي 10
ﻣﯿ ﻟوﺮﮑ ﯿ ﭘ ﺲﻧﺎﺳا زا ﺮﺘ ﯿ
ﺤﻣ ﺢﻄﺳ ﺮﺑزﺎ ﯿ
ﺖﺸﮐ ﻂ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ﻪﺑ
ﺮﺘﺳا ﺲﻨﭘ ﮏﻤﮐ ﯾ
ﺪﺷ هداد راﺮﻗ ﻞ .
ﻮﺳ ﯾﻪ ﺎﻫ ي ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﭼرﺎﻗ و ﯽ
ﺗﺮﺗ ﻪﺑ ﯿ ﺐ 24 و 72 ﺖﻋﺎﺳ ﺎﻫﺎﻣد رد ي 37 و 25 ﻪﺟرد ﺘﻧﺎﺳ ﯽ دﺮﮔ ﻪﺑﻮﮑﻧا داﺮﮔ ﯾﺪ
. ﻃ زا ﺲﭘ ﯽ ا ﯾ ﻪﻟﺎﻫ ،نﺎﻣز ﻦ فﺎﻔﺷ
اﯾ دﺎﺠ د فاﺮﻃا رد هﺪﺷ ﯾ
ﺪﺷر مﺪﻋ ﺮﮕﻧﺎﺸﻧ ﮏﺴ
ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي ﭘ ﺲﻧﺎﺳا رﻮﻀﺣ رد از ﯿ
ﻣ زﺎ ﯽ ﺪﺷﺎﺑ .
ا فﺎﻔﺷ ﻪﻟﺎﻫ ﯾ
فاﺮﻃا رد هﺪﺷ دﺎﺠ د
ﯾ ﮏﺴ ﻂﺧ ﻂﺳﻮﺗ ﺎﻫ ﺶﮐ
هزاﺪﻧا ﺮﯿﮔ ي ﻠﯿﻣ ترﻮﺻ ﻪﺑ و ﯽ
ﺮﺘﻣ ي ﺪﺷ شراﺰﮔ .
ﺖﺳا ﺮﮐذ ﻞﺑﺎﻗ
ﺑوﺮﮑﯿﻣﺪﺿ ﺮﺛا ﻪﮐ ﯽ
ﭘ ﺲﻧﺎﺳا ﯿ ور زﺎ ي ﻮﺳ ﺮﻫ ﯾ ﻣ ﻪ ﯿ ﺑوﺮﮑ ﯽ ﻪﺳ رد
ﺪﺷ مﺎﺠﻧا راﺮﮑﺗ ]
13 و 14 [ .
2 - 5 - رﺎﮔآ ﮏﻫﺎﭼ
ﻘﻃﺎﻨﻣ رد ،ﮏﻫﺎﭼ شور رد ﯽ
ﺤﻣ زا ﯿ ﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂ ﯿ
رﺎﮔآ نﻮﺘﻨ
اﺮﺑ ي ﻮﺳ ﯾﻪ ﺎﻫ ي ﺮﺘﮐﺎﺑ ي ﺤﻣ زا و ﯿ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ ﺖﺸﮐ ﻂ
اﺮﺑرﺎﮔآ ي ﻮﺳ ﯾ ﻗ ﻪ ﮏﻫﺎﭼ ،چرﺎ ﺎﻫ
ﯾ ﯽ ﺮﻄﻗ ﺎﺑ 6 ﻣ ﯿﻠ ﯽ ﻪﺑ ﺮﺘﻣ ﺳو ﯿ ﻪﻠ
ﺎﻬﺘﻧا ي ﭘ ﯽ ا رﻮﺘﺳﺎﭘ ﺖﭘ ﯾ
ﺪﺷ دﺎﺠ . ﻞﺧاد ﺮﻫ ﺮﻠﭙﻤﺳﺎﺑ ﮏﻫﺎﭼ 20
ﻣﯿ ﻟوﺮﮑ ﯿ ﺮﺘ ﭘ ﺲﻧﺎﺳا ﯿ زﺎ ﺪﺷ ﻪﺘﺨﯾر .
ﯾ ﻧ ﮏﻫﺎﭼ ﮏ ﯿ
ناﻮﻨﻋ ﻪﺑ ﺰ
ﭘ ﺲﻧﺎﺳا ﺪﻗﺎﻓ لﺮﺘﻨﮐ ﻪﻧﻮﻤﻧ ﯿ
ﻧ زﺎ ﯿ ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﺰ .
ﺤﻣ ﯿ ﻂ
وﺎﺣ ﺖﺸﮐ ي
ﺮﺘﮐﺎﺑ ي ﺗﺮﺗ ﻪﺑ چرﺎﻗ و ﯿ
ﺎﻣد رد ﺐ ي
37 ﻪﺟرد
ﺘﻧﺎﺳ ﯽ تﺪﻣ ﻪﺑ داﺮﮔ 24
و ﺖﻋﺎﺳ 25
ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ
72 مﺮﮔ ﺖﻋﺎﺳ ﻪﻧﺎﺧ
راﺬﮔ ي دﺮﮔ ﯾﺪ . ﺲﭘ زا ﻃ ﯽ ﺳﺎﺑﻮﮑﻧا نﺎﻣز ﯿ
نﻮ
ﻣﯿ ناﺰ ﻖﻃﺎﻨﻣ يرﺎﻬﻣ درﻮﻣ زرا ﯾﺑﺎ ﯽ و ﺖﻓﺮﮔ راﺮﻗ مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ
ﻂﺧ ﺎﺑ ﺪﺷر سﺎﺳاﺮﺑ ﺶﮐ
ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ﺪﺷ ﺖﺒﺛ ] 15 و 16 [ .
2 - 6 - ﻌﺗ ﯿﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦ ﯽ
ﺲﻧﺎﺳا
ﭘﯿ زﺎ
ﻌﺗ ﯿﯿ ﻣ ﺪﺷر زا هﺪﻨﻨﮐ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦ ﯿ
ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي
ﺑﯿ رﺎﻤ ي ﮏﻫﺎﭼ ﺎﺑ از 96
ﻪﻧﺎﺧ ا ي فﺮﻌﻣ زا هدﺎﻔﺘﺳا ﺎﺑ و ﺮﺗ
ي ﻨﻓ ﯿ ﻞ
ﻟوزاﺮﺘﺗ ﯿ اﺮﻠﮐ مﻮ ﯾ ﺪ ﯾ ﻣ شور نﺎﻤﻫ ﺎ ﯿ
ادوﺮﮑ ﯾ ﻌﺗ ثاﺮﺑ ﻦﺷﻮﻠ ﯿﯿ
ﻦ
ﺪﺷ . ا رد ﯾ ﺖﻈﻠﻏ شور ﻦ ﺎﻫ
ي ﻟاﻮﺘﻣ ﯽ ﭘ ﺲﻧﺎﺳا زا ﯿ
ﺎﺑ زﺎ
ﻗر ﯿ ﻖ زﺎﺳ ي ﺤﻣ رد ﯿ ﻣ ﺖﺸﮐ ﻂ ﯿ
ﺑوﺮﮑ ﯽ ) ﺮﻟﻮﻣ ﻫ ﯿ و ثاﺮﺑ نﻮﺘﻨ
ثاﺮﺑ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ (
ﻬﺗ ﯿ ﺪﺷ ﻪ . ﺮﻫ نورد ﯾ ﮏﻫﺎﭼ زا ﮏ ﺎﻫ
ﻣﯿ ناﺰ 100 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺖﻈﻠﻏ زا ﺮﺘ ﺎﻫ
ي و ﺲﻧﺎﺳا ﻒﻠﺘﺨﻣ 10
ﻣﯿ ﻟوﺮﮑ ﯿ ﻮﺳ زا ﺮﺘ ﯾ ﻣ دراﺪﻧﺎﺘﺳا ﻪ ﯿ
ﺑوﺮﮑ ﯽ ﻧ لدﺎﻌﻣ ﻪﮐ ﯿ
ﮏﻣ ﻢ ﺪﻨﻟرﺎﻓ
ﻘﻠﺗ دﻮﺑ ﯿ ﺪﺷ ﺢ . ﮏﻫﺎﭼ 96 ﻪﻧﺎﺧ ا ي وﺎﺣ ي ﺮﺘﮐﺎﺑ ي ﻪﺑ چرﺎﻗ و
ﺗﺮﺗ ﻪﺑ ﻪﻧﺎﮔاﺪﺟ رﻮﻃ ﯿ
ﺎﻣد رد ﺐ ي 37 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ
24 و ﺖﻋﺎﺳ 25
ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ 72
ﺖﻋﺎﺳ
مﺮﮔ ﻪﻧﺎﺧ راﺬﮔ ي دﺮﮔ ﯾﺪ . ﺲﭘ زا ﻃ ﯽ ﺳﺎﺑﻮﮑﻧا نﺎﻣز ﯿ
اﺮﺑ نﻮ ي
ﻮﺳ ﯾﻪ ﺎﻫ ي ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ لﻮﻠﺤﻣ 5 ﺪﺻرد فﺮﻌﻣ زا ﺮﺗ
ي ﻨﻓ ﯿ ﻞ
ﻟوزاﺮﺘﺗ ﯿ اﺮﻠﮐ مﻮ ﯾ ﻣ ﻪﺑ ﺪ ﯿ ناﺰ 10 ﻣ ﯿ ﻟوﺮﮑ ﯿ دادﺪﺠﻣ و هﺪﺷ ﻪﻓﺎﺿا ﺮﺘ
تﺪﻣ ﻪﺑ 15
ﻗد ﯿ ﻪﻧﺎﺨﻣﺮﮔ ﻪﻘ ﺬﮔ
را ي ﺎﻣد رد ي 37 ﻪﺟرد
ﺘﻧﺎﺳ ﯽ ﺪﺷ مﺎﺠﻧا داﺮﮔ .
ﻟوا ﯿ ﺘﻈﻠﻏ ﻦ ﯽ ﺮﺘﮐﺎﺑ ﺪﺷر نآ رد ﻪﮐ ي
ور ي
ﮑﺸﺗ ﺰﻣﺮﻗ ﮓﻧر و هداﺪﻧ ﯿ
ﻧاﻮﻨﻋ ﻪﺑ ﺪﺸﻧ ﻞ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺤ
ﺪﺷر زا هﺪﻨﻨﮐ ﺪﯾدﺮﮔ شراﺰﮔ
. ﺎﻣزآ ﯽﻣﺎﻤﺗ ﯾ
ﺶ ﻪﺳ رد ﺎﻫ
ﺖﻓﺮﯾﺬﭘ مﺎﺠﻧاراﺮﮑﺗ ]
15 و 17 [ .
2 - 7 - ﻌﺗ ﯿﯿ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦ ﯽ
ﻌﺗ زا ﺪﻌﺑ ﯿﯿ ﻞﻗاﺪﺣ ﻦ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﯽ
اﺮﺑ ي ﻮﺳ ﯾﻪ ﺎﻫ ي ﺮﺘﮐﺎﺑ ي
ﮏﻫﺎﭼ زا ،چرﺎﻗ و ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ
ﯽ ﺖﻤﺳ ﻪﺑ
ﺖﻈﻠﻏ ﺎﻫ ي ﻣ ﺮﺗﻻﺎﺑ ﯿ ناﺰ 100 ﻣ ﯿ ﻟوﺮﮑ ﯿ و ﻪﺘﺷادﺮﺑ ﺮﻠﭙﻤﺳ ﻂﺳﻮﺗ ﺮﺘ
ﺤﻣ ﺢﻄﺳ ﺮﺑ ﯿ
ﻂ ﺎﻫ ي ﻣ ﺖﺸﮐ ﯿ ﺑوﺮﮑ ﯽ ) ﻫ ﺮﻟﻮﻣ ﯿ اﺮﺑ رﺎﮔآ نﻮﺘﻨ ي
ﻮﺳ ﯾﻪ ﺎﻫ ي ﺮﺘﮐﺎﺑ ي اﺮﺑ رﺎﮔآ زوﺮﺘﺴﮐد زوﺮﺑﺎﺳ و ي
ﻮﺳ ﯾ چرﺎﻗ ﻪ (
دﺮﮔ ﺶﺨﭘ ﯾﺪ
. ﺮﺘﭘ ي د ﯾ ﺶ ﺎﻫ ي وﺎﺣ ي ﺮﺘﮐﺎﺑ ي رﻮﻃ ﻪﺑ چرﺎﻗ و
ﺗﺮﺗ ﻪﺑ ﻪﻧﺎﮔاﺪﺟ ﯿ
ﺎﻣد رد ﺐ ي 37 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ 24
و ﺖﻋﺎﺳ 25
ﺘﻧﺎﺳ ﻪﺟرد ﯽ
تﺪﻣ ﻪﺑ داﺮﮔ 72
ﺖﻋﺎﺳ
مﺮﮔ ﻪﻧﺎﺧ راﺬﮔ ي دﺮﮔ ﯾﺪ . ﻃزﺎﺴﭘ ﯽ ﺳﺎﺑﻮﮑﻧا نﺎﻣز ﯿ
ﻨﻠﮐ ﺪﺷر نﻮ ﯽ
ﺤﻣ ﺢﻄﺳ ﺮﺑ ﯿ
زرا درﻮﻣ ﻢﺸﭼ ﻂﺳﻮﺗ ﺖﺸﮐ ﻂ ﯾﺑﺎ
ﯽ ﺖﻓﺮﮔ راﺮﻗ .
ﻟوا ﯿ ﺮﺘﭘ ﻦ ي د ﯾ ﻪﮐ ﺶ ﻨﻠﮐ نآ رد ﯽ ﻞﻗاﺪﺣ ناﻮﻨﻋ ﻪﺑ ﺪﺸﻧ هﺪﻫﺎﺸﻣ
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﯽ
ﭘ ﺲﻧﺎﺳا ﯿ ﺪﺷ شراﺰﮔ زﺎ ]
15 و 18 [.
2 - 8 - ﻟﺎﻧآ ﯿ رﺎﻣآ ﺰ ي
ﺰﺠﺗ ﯾﻪ و ﻠﺤﺗ ﯿ ﻞ هداد مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ رﺎﻣآ راﺰﻓا
ي SPSS
ﻪﺨﺴﻧ 16 ﺖﻓﺮﮔ ترﻮﺻ ﺎﻘﻣ ﺖﻬﺟ .
ﯾ ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﻦ ) رﺎﺑ ﻪﺳ ﻞﻣﺎﺷ ﺎﻣزآ راﺮﮑﺗ
ﯾ ﺪﻧدﻮﺑ ﺶ ( ﻟﺎﻧآ نﻮﻣزآ زا ، ﯿ
راو ﺰ ﯾ ﺲﻧﺎ ﯾ ﻪﻓﺮﻃ ﮏ
ANOVA ﺪﺷ هدﺎﻔﺘﺳا
.
3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ
3 - 1 - ﺎﺳﺎﻨﺷ ﯾ ﯽ ﮐﺮﺗ ﯿ ﺷ تﺎﺒ ﯿﻤ ﯿﺎ ﯾ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ
زﺎ
ﺲﻧﺎﺳا ﺰﯿﻟﺎﻧآ ﺞﯾﺎﺘﻧ ﭘﯿ
زﺎ ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ هﺎﮕﺘﺳد ﻪﻠﯿﺳو ﻪﺑ ﯽ
زﺎﮔ ي
ﻃ ﻪﺑ ﻞﺼﺘﻣ ﯿ
ﻒ ﻣﺮﺟ ﺞﻨﺳ ﯽ لوﺪﺟ رد 1
ﺖﺳا هﺪﺷ هدروآ ، .
ﺎﺘﻧ ﯾ ﺞ
عﻮﻤﺠﻣ رد داد نﺎﺸﻧ 20
ﺮﻘﺗ ﯽﯾﺎﯿﻤﯿﺷ راّﺮﻓ هدﺎﻣ ﯾًﺎﺒ
100 ﺪﺻرد
ﮐﺮﺗ ﯿ تﺎﺒ ﭘ ﺲﻧﺎﺳا ﯿ ﮑﺸﺗ ار زﺎ ﯿ هداد ﻞ ﺪﻧا . ﮐﺮﺗ ﯿ تﺎﺒ Trisulfide
dipropyl ،
Disulfide dipropyl
، n-Decane و
Furfuryl methyl sulfide ﺗﺮﺗ ﻪﺑ
ﯿ ﺎﺑ ﺐ 67 / 20 ، 18 / 16 ،
51 / 13 و 23 / 10 اﺰﺟا ﺪﺻرد ي
ﻠﺻا ﯽ ﮑﺸﺗ ﯿ ﭘ ﺲﻧﺎﺳا هﺪﻨﻫد ﻞ ﯿ
زﺎ
ﺪﻧدﻮﺑ . ﺎﻘﻣ ﯾ ﺎﺘﻧ ﻪﺴ ﯾ ﺶﻫوﮋﭘ ﺎﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺞ Liguori
و
نارﺎﮑﻤﻫ )
2017 ( دوﺪﺣ ﺎﺗ ي ﺖﺷاد ﺖﻘﺑﺎﻄﻣ ]
19 [ . فﻼﺘﺧا
ﺑ هﺪﺷ هﺪﻫﺎﺸﻣ ﯿ
ﮐﺮﺗ ﺪﺻرد و عﻮﻧ ﻦ ﯿ
ﺎﺳﺎﻨﺷ تﺎﺒ ﯾ
ﯽ ار هﺪﺷ
ﻣ ﯽ ﻣ فﻼﺘﺧا ﻪﺑ ناﻮﺗ ﯿ
راو عﻮﻧ نﺎ ﯾ
اﺮﺷ ،ﺖﺸﮐ ﻞﺤﻣ ،ﻪﺘ ﯾ
و بآ ﻂ
اﻮﻫ ﯾ ﯽ و ﺖﺷادﺮﺑ نﺎﻣز ، ...
ﺖﺴﻧاد ﻂﺒﺗﺮﻣ ]
20 [ . ﺎﺘﻧ ﯾ ا ﺞ ﯾ ﻦ
ﮐﺮﺗ ﻪﮐ داد نﺎﺸﻧ ﺶﻫوﮋﭘ ﯿ
ﻔﻟﻮﺳ تﺎﺒ ﯿ ﺪ ي ﻠﺻا ﺶﺨﺑ ﯽ
ﺲﻧﺎﺳا
ﭘﯿ ﮑﺸﺗ ار زﺎ ﯿ ﻣ ﻞ ﯽ ﺪﻨﻫد .
Table 1 Chemical constituents of Allium cepa essential oil
No. Compounds Retention time (min) %
1 Methyl propyl disulfide 10.43 2.44
2 Furfuryl methyl sulfide 14.48 10.23
3 n-Decane 14.69 13.51
4 3-(Methylthio) propanoic acid methyl ester 16.33 4.06
5 Diallyl disulphide 20.58 1.1
6 Undecane 22.45 1.90
7 Disulfide, dipropyl 22.96 16.18
8 1-norvaline, N-propargyloxycarbonyl-, nonyl ester 26.45 3.36
9 Dodecane 31.1 7.02
10 Allyl trisulfide 39.45 0.92
11 Tridecane 39.87 1.84
12 Trisulfide,dipropyl 41.75 20.67
13 Furfuryl disulfide 46.24 0.79
14 Naphtho[2,3-b]thiophene-4,9-dione 47.20 1.06
15 Tetradecane 48.40 1.81
16 Sulfaguanidine 61.11 6.74
17 β-monoolein 114.06 1.65
18 Glycerol 1-palmitate 115.16 1.39
19 Isopropyl linoleate 116.36 0.49
20 n-propyl 11-octadecenoate 116.54 2.81
3 - 1 - ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ
ﺑوﺮﮑ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ
زﺎ
ﻟﺎﻌﻓ ﺞﯾﺎﺘﻧ ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﭘ ﺲﻧﺎﺳا ﯿ شور ﻪﺑ زﺎ ﺎﻫ
ي د ﯾ ﮏﺴ
دﯾ ﻔﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،رﺎﮔآ ﮏﻫﺎﭼ ،رﺎﮔآ نژﻮ ﯽ
و
ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
لوﺪﺟ رد 2
ﺖﺳا هﺪﺷ هدروآ ، .
ﺎﺘﻧ ﯾ ﺞ
د شور رد ﻪﮐ داد نﺎﺸﻧ ﯾ
د ﮏﺴ ﯾﻔ ﯿ نژﻮ
، ﭘ ﺲﻧﺎﺳا ﯿ ﺑ زﺎ ﯿ ﺮﺘﺸ ﯾ ﻦ
ﺛﺎﺗ ﯿ ﺮﺘﮐﺎﺑ ﺮﺑ ار ﺮ ي
ﺎﻫ ﻓﺎﺘﺳا ي ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﻼﻧﻮﻤﻟﺎﺳ و
ﺗﯿ ﻔ ﯽ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﺎﺑ 11
ﻣ ﯿﻠ ﯽ ﺖﺷاد ﺮﺘﻣ .
ﻢﮐ ﺮﺗ ﯾ ﺮﻄﻗ ﻦ
د شور رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﯾ
د ﮏﺴ ﯾﻔ ﯿ ﺮﻤﺨﻣ ﻪﺑ طﻮﺑﺮﻣ نژﻮ
ﺪﻧﺎﮐ ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ دﻮﺑ . ﺎﺘﻧ ﯾ ﻪﮐ داد نﺎﺸﻧ رﺎﮔآ ﮏﻫﺎﭼ نﻮﻣزآ ﺞ
ﭘ ﺲﻧﺎﺳا ﯿ ﺑ زﺎ ﯿ ﺮﺘﺸ ﯾ ﻢﮐ و ﻦ ﺮﺗ ﯾ ﺛﺎﺗ ﻦ ﯿ ﺗﺮﺗ ﻪﺑ ار ﺮ ﯿ ﺮﺘﮐﺎﺑ ﺮﺑ ﺐ ي
مﺮﮔ
ﺖﺒﺜﻣ ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﺮﻄﻗ ﺎﺑ
15 ﻣ ﯿﻠ ﯽ ﺮﺘﮐﺎﺑ و ﺮﺘﻣ ي
ﻔﻨﻣ مﺮﮔ ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ ﯽ
ﯾ ازﻮﻨ ﺮﻄﻗ ﺎﺑ 10 ﻣ ﯿﻠ ﯽ ﺖﺷاد ﺮﺘﻣ .
ﺎﻘﻣ ﯾ ﺎﺘﻧ ﻪﺴ ﯾ ﻣ ﺞ ﯿ شور نﺎ ﺎﻫ ي د ﯾ د ﮏﺴ ﯾﻔ ﯿ ﮏﻫﺎﭼ و رﺎﮔآ نژﻮ
ﻪﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ رﺎﮔآ ﮏﻫﺎﭼ شور رد ﻪﮐ داد نﺎﺸﻧ رﺎﮔآ ﻨﻌﻣ رﻮﻃ ﯽ راد ي د شور ﻪﺑ ﺖﺒﺴﻧ ﯾ
د ﮏﺴ ﯾﻔ ﯿ ﺮﺘﮔرﺰﺑ رﺎﮔآ نژﻮ
ﺖﺳا . ﻟد ﯿ ا ﻞ ﯾ ﻣ ار ﺮﻣا ﻦ ﯽ
ﻂﺒﺗﺮﻣ رﺎﮐ مﺎﺠﻧا شور توﺎﻔﺗ ﻪﺑ ناﻮﺗ
ﺖﺴﻧاد . د شور رد ﯾ
د ﮏﺴ ﯾﻔ ﯿ ژﻮ هدﺎﻣ ﺖﺳا مزﻻ رﺎﮔآ ن
ﻣﺪﺿ ﯿ بوﺮﮑ ) ﭘ ﺲﻧﺎﺳا ﯿ زﺎ ( د زا ﯾ ﺬﻏﺎﮐ ﮏﺴ ي
ﺤﻣ ﺢﻄﺳ ﻪﺑ ﯿ
ﻂ
ﻮﺳ ﺮﺑ ار دﻮﺧ ﺮﺛا و هﺪﺷ ﺮﺸﺘﻨﻣ ﺖﺸﮐ ﯾﻪ
ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي نﺎﺸﻧ از
ﻟﺎﺣ رد ﺪﻫد ﯽ
د رﺎﮔآ ﮏﻫﺎﭼ شور رد ﻪﮐ ﯾ
ﺬﻏﺎﮐ ﮏﺴ ي
فﺬﺣ
ﻘﺘﺴﻣ رﻮﻃ ﻪﺑ ﺲﻧﺎﺳا و هﺪﺷ ﯿ
ﻮﺳ ﺎﺑ ﻢ ﯾﻪ ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي سﺎﻤﺗ رد از
ﺖﺳا . ﺎﺘﻧ ﯾ ﻣ هﺪﻣآ ﺖﺳد ﻪﺑ ﺞ ﯿ
ﺧا نﺎ هﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ فﻼﺘ
ﺎﺳ ﺎﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد هﺪﻫﺎﺸﻣ ﯾ
ﺶﻫوﮋﭘ ﺮ ﺖﺷاد ﺖﻘﺑﺎﻄﻣ ﺎﻫ .
ﻞﮑﺷ 1 ﺎﻤﻧ ، ﯾ ﯽ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣزا ﯽ
ﭘ ﺲﻧﺎﺳا ﯿ ﺮﺑ زﺎ
ﻮﺳ ﯾﻪ ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي ﺖﺳا هداد نﺎﺸﻧ ار از .
ﺎﺘﻧ ﯾ ﻞﻗاﺪﺣ نﻮﻣزآ ﺞ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﯽ
ﭘ ﺲﻧﺎﺳا ﺪﺷر زا ﯿ
اﺮﺑ زﺎ ﻓﺎﺘﺳا ي ﯿ سﻮﮐﻮﮐﻮﻠ
سﻮﺋروا ﻟﯿ ، ﺮﺘﺴ ﯾ ا ﺎ ﯾ ﮐﻮﻨ اﻮ ﺷﺮﺷا ، ﯿ ﻠﮐ ﺎ ﯽ ﺗ ﻼﻧﻮﻤﻟﺎﺳ ، ﯿﻔ
ﯽ ،
ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ ﯾ
ازﻮﻨ ﺪﻧﺎﮐ و ﯾ ﺒﻟآ اﺪ ﯿ ﺲﻨﮑ ﺗﺮﺗ ﻪﺑ ﯿ ﺐ 512 ،
512 ، 512 ، 512 ، 512 و 1024 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ دﻮﺑ ﺮﺘ . ﺎﺘﻧ
ﯾ ﺞ نﻮﻣزآ ﻞﻗاﺪﺣ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﯽ
ﺲﻧﺎﺳا ﭘ ﯿ زﺎ اﺮﺑ ي
ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﻟﯿ ،
ﺮﺘﺴ ﯾ ا ﺎ ﯾ اﻮﮐﻮﻨ ﺷﺮﺷا ، ﯿ ﻠﮐ ﺎ ﯽ ،
ﺗ ﻼﻧﻮﻤﻟﺎﺳ ﯿﻔ
ﯽ ژوﺮﺋا سﺎﻧﻮﻣودﻮﺳ و ﯾ
ازﻮﻨ زا ﺮﺘﮔرﺰﺑ 2048
ﻣﯿ ﻠ ﯽ ﺮﺑ مﺮﮔ ﻣ ﯿﻠ ﯽ ﻟﯿ دﻮﺑ ﺮﺘ . ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
اﺮﺑ ي ﻮﺳ ﯾ ﻪ
ﭼرﺎﻗ ﯽ 2048 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ
ﯽ ﻟﯿ دﻮﺑ ﺮﺘ . ﻟﺎﮑﺑ ﯽ و
نارﺎﮑﻤﻫ ) 2008 ( ﻟﺎﻌﻓ ﺪﻧدﺮﮐ شراﺰﮔ ، ﯿ
ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﺲﻧﺎﺳا ﺎﻫ
ﻪﺑ ﻪﺘﺴﺑاو ﯾ
ﻧﺎﮑﻣ ﮏ ﯿ ﻧ ﻢﺴ ﯿ ﻧﺎﮑﻣ ﻪﮑﻠﺑ و ﺖﺴ ﯿ
ﻢﺴ رد عﻮﻨﺘﻣ يﺎﻫ
ﻮﮑﻟﻮﻣ ﺢﻄﺳ ﻟ
ﯽ ا رد ﯾ ا ﺶﻘﻧ عﻮﺿﻮﻣ ﻦ ﯾ
ﻣ ﺎﻔ ﯽ ﺪﻨﻨﮐ . ﯾ ﮑ ﯽ هار زا -
ﺳآ ،ﻦﮑﻤﻣ يﺎﻫ ﯿ
ﻏ نﺪﻧﺎﺳر ﺐ ﯿ
لﻮﻠﺳ يﺎﺸﻏ ﻪﺑ ﺖﺸﮔﺮﺑ ﻞﺑﺎﻗﺮ
ﺳ داﻮﻣ ﺖﺸﻧ ﺚﻋﺎﺑ ﻪﮐ ﺖﺳا يﺮﺘﮐﺎﺑ ﯿ
ﻤﺳﻼﭘﻮﺘ ﯽ ، ﯾ نﻮ ا و ﺎﻫ ﯾ دﺎﺠ
ﺎﻬﻧ رد ﻪﮐ دﻮﺷ ﺰﮐﻮﻠﮔ ﺪﻨﻧﺎﻣ يژﺮﻧا يﺎﻫاﺮﺘﺴﺑﻮﺳ دﻮﺒﻤﮐ ﯾ
ﺮﺠﻨﻣ ﺖ
ﻟ ﻪﺑ ﯿ م نآ گﺮﻣ و يﺮﺘﮐﺎﺑ نﺪﺷ ﺰ ﯾ
دﻮﺸ . ﯾ ﻟﺎﻤﺘﺣا هاﺮﮑ ﯽ
د ﯾ ،ﺮﮕ
ﻟﻮﺗ رﺎﻬﻣ ﯿ ﻣآ ﺪ ﯿ ﻟﻮﺗ ﻒﻗﻮﺗ ﺚﻋﺎﺑ ﻪﮐ ﺖﺳا زﺎﺌﺗوﺮﭘ و زﻼ ﯿ
و ﻢﺳ ﺪ
ﺮﺟ ﯾ ﻣ نوﺮﺘﮑﻟا نﺎ ﯽ
ا رد ﻪﮐ دﻮﺷ ﯾ
و ﻪﺘﺸﮔ ﺪﻘﻌﻨﻣ لﻮﻠﺳ ﺖﻟﺎﺣ ﻦ
ﻣ ﯽ ﻣﯿ دﺮ . ﻟﺎﻌﻓ ﯿ ﺪﺿ ﺖ ﭼرﺎﻗ ﯽ ﻟﺎﻌﻓ ﻪﺑﺎﺸﻣ ﯿ
ﺪﺿ ﺖ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ،ﺖﺳا
ﺪﭘ ود ﺎﻣا ﯾ د هﺪ ﯾ ﻟﺎﻌﻓ رﺎﻬﻣ رد ﺮﮕ ﯿ
ﺑ ﻞﺑﺎﻗ ﺮﻤﺨﻣ ﺖ ﯿ
ﻪﮐ ﺖﺳا نﺎ
ا ﻞﻣﺎﺷ ﯾ دﺎﺠ ﭘ ﺎﺸﻏ يﻮﺳ ود ﺮﻫ رد pH ﺎﻤﺳﻼ
ﯾ ﯽ د و ﯾ رﺎﻬﻣ يﺮﮕ
ﻟﻮﺗ ﯿ ﻟﺎﻌﻓ رد ﻪﭽﻧآ ﻪﺑﺎﺸﻣ يژﺮﻧا ﺪ ﯿ
ﺪﺿ ﺖ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ خر
ﻣ ﯽ ﺪﻫد ] 21 [.
ﻠﺒﮑﻨﺑ ﯿ ﺎ ) 2004 (،
ﻟﺎﻌﻓ ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ ﻦﻏور ﺎﻫ ي
ﭘ ﺲﻧﺎﺳا ﯿ ﺳ و زﺎ ﯿ ﺖﻈﻠﻏ رد ﺮ ﺎﻫ
ي 50 ، 100 ، 200 ، 300 و ، 500
ﻣﯿ ﻠ ﯽ ﺳرﺮﺑ درﻮﻣ ار مﺮﮔ ﯽ
داد راﺮﻗ . اﯾ ﺳرﺮﺑ ﻦ ﯽ ﺮﺘﮐﺎﺑ ﺮﺑ ي ﺎﻫ ي
ﻓﺎﺘﺳا ﯿ ﻮﮐﻮﮐﻮﻠ سﻮﺋروا س ﻼﻧﻮﻤﻟﺎﺳ و
ترﻮﺻ چرﺎﻗ عﻮﻧ ﻪﺳ و
ﺖﻓﺮﮔ . ﺎﺘﻧ ﯾ ا ﺞ ﯾ ﻣﺪﺿ ﺮﺛا ﺶﻫوﮋﭘ ﻦ ﯿ
ﺑوﺮﮑ ﯽ ﺎﺗ ار ﯾﯿ دﻮﻤﻧ ﺪ ] 22 [.
ﻠﮐ رﻮﻃ ﻪﺑ ﯽ
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﺮﺘﮐﺎﺑ ﻪﺑ ﺖﺒﺴﻧ ﺖﺒﺜﻣ مﺮﮔ ي
ﺎﻫ ي مﺮﮔ
ﻔﻨﻣ ﯽ ﺳﺎﺴﺣ زا ﯿ
ﺑ ﺖ ﯿ ﺮﺘﺸ ي ﭘ ﺲﻧﺎﺳا ﻪﺑ ﺖﺒﺴﻧ ﯿ
رادرﻮﺧﺮﺑ زﺎ
ﺪﻧدﻮﺑ . ﻟد ﯿ ا ﻞ ﯾ ﻣ ار ﺮﻣا ﻦ ﯽ
د توﺎﻔﺗ ﻪﺑ ناﻮﺗ ﯾ
ﻟﻮﻠﺳ هراﻮ ﯽ ﻣ ﯿ نﺎ
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﺮﺘﮐﺎﺑ ﺎﺑ ﺖﺒﺜﻣ مﺮﮔ ي
ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ ﻂﺒﺗﺮﻣ
ﺖﺴﻧاد ] 20 [.
Table 2. In vitroantimicrobial activity of Allium cepa essential oil
Antimicrobial assays Microbial strains Disc diffusion agar
(mm)
Well diffusion agar
(mm) MIC (mg/mL) MBC/MFC
(mg/mL)
Staphylococcus aureus 11.00 ± 0.60 15.00 ± 0.50 512 >2048
Listeria innocua 8.50 ± 0.45 11.00 ± 0.45 512 >2048
Escherichia coli 10.50 ± 0.50 14.00 ± 0.55 512 >2048
Salmonella typhi 11.00 ± 0.60 12.00 ± 0.55 512 >2048
Pseudomonas aeruginosa 10.00 ± 0.50 10.00 ± 0.50 512 >2048
Candidaalbicans 8.00 ± 0.24 11.00 ± 0.55 1024 2048
Values are expressed as mean ±standard deviations, n = 3
Fig 1 Minimum inhibitory concentration of the Allium cepa essential oil by microdilution broth
method.
4 - ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي ﺎﻬﻧ ﯾ ﯽ
لﺎﺳ رد ﺎﻫ ي ﺧا ﯿ ﻠﻣاﻮﻋﺮ ﯽ ﺑﺪﻨﻧﺎﻤﻫ ﯿ رﺎﻤ ي ﺎﻫ ي ﺷﺎﻧ ﯽ فﺮﺼﻣ زا
اﺬﻏداﻮﻣ ﯾ ﯽ اﺰﻓا ،هدﻮﻟآ ﯾ
ﻣ ﺖﻣوﺎﻘﻣ ﺶ ﯿ
ﺑوﺮﮑ ﯿ ﻪﺑ ﺖﺒﺴﻨ
ﺘﻧآ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺬﭘ و ﺎﻫ ﯾ ﺘﻨﺳ ﺐﻃ شﺮ ﯿ
ﻧﺎﻣرد تﺎﻣﺪﺧ رﺪ ﯽ
ﺐﺒﺳ
هﺪﺷ ﺎﺟ ﻪﺑ ﺎﺗﺪﻧا ﯾ
ﮔرﺎﮐ ﻪﺒ ﯿﺮ ﯾ ﺘﻧآ زﺎ ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﺞ
ﻧﺎﻣرد ﯽ ﮐﺮﺗ زا هدﺎﻔﺘﺳا،
ﯿ ﺗﺎﺒ ﯽ ﮔ ﺄﺸﻨﻣ ﺎﺑ ﯿ
ﻫﺎ ﯽ ﻞﻣاﻮﻋ ناﻮﻨﻋ ﻪﺑ
ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﺎﺟ و ﯾ ﺰﮕ ﯾ هﺪﻧراﺪﻬﮕﻧ ﻦ ﺎﻫ
ي ﺷ ﯿﻤ ﯿﺎ ﯾ ﯽ ﻪﺟﻮﺗ درﻮﻣ
ﮔ راﺮﻗ ﯿ ﺪﻧﺮ . ا رد ﯾ ﭘ ﺲﻧﺎﺳا ﺶﻫوﮋﭘ ﻦ ﯿ
ﺳرﺮﺑ درﻮﻣ زﺎ ﯽ
راﺮﻗ
ﺖﻓﺮﮔ . ﻠﮐ رﻮﻃ ﻪﺑ ﯽ ﺎﺘﻧ ﯾ ﺻﺎﺣ ﺞ ا زا ﻞ ﯾ ﺑﺮﺠﺗ ﺶﻫوﮋﭘ ﻦ ﯽ
نﺎﺸﻧ
ﻣﺪﺿ ﺮﺛا ﻪﮐ داد ﯿ
ﺑوﺮﮑ ﯿ ﭘ ﺲﻧﺎﺳﺎ ﯿ دوﺪﺣ ﺎﺗ زﺎ ي
عﻮﻧ ﻪﺑ ﻪﺘﺴﺑ
ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻤﻤﺴ ﯽ توﺎﻔﺘﻣ ﺪﻧاﻮﺗ رﻮﻃ ﻪﺑ ،ﺪﺷﺎﺑ
ي ﺮﺘﮐﺎﺑ ﻪﮐ ي ﺎﻫ ي
ﺖﺒﺜﻣ مﺮﮔ ﻓﺎﺘﺳا)
ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﻟﯿو
ﺮﺘﺴ ﯾ ا ﺎ ﯾ اﻮﮐﻮﻨ ( رد
ﺎﻘﻣ ﯾ ﺮﺘﮐﺎﺑ ﺎﺑ ﻪﺴ ي
ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ
ﺗ ﻼﻧﻮﻤﻟﺎﺳ) ﯿﻔ
ﯽ ،
ﺳﺎﻧﻮﻣدﻮﺳ ازﻮﻨﯾژورﻮﺋﺂ ﺷا و
ﺷﺮ ﯿ ﻠﮐﺎ ﯽ ( ﺳﺎﺴﺣ زا ﯿ ﺑ ﺖ ﯿ ﺮﺘﺸ ي
ﺪﻧدﻮﺑ رادرﻮﺧﺮﺑ .
ﭘﯿ ﻣ دﺎﻬﻨﺸ ﯽ ﺶﻫوﮋﭘ ،ﻪﻣادا رد دﻮﺷ ﺎﻫ
ي
دﺪﻌﺘﻣ ي ﻣز رد ﯿ ﭘ ﺲﻧﺎﺳا زا هدﺎﻔﺘﺳا ﻪﻨ ﯿ
ﺎﺳ ﺮﺑ زﺎ ﯾ
ﺮ
ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي ﻨﭽﻤﻫ و از ﯿ ا ﺶﻨﮑﻤﻫﺮﺑ ﻦ ﯾ
ﺲﻧﺎﺳا ﻦ
داﺪﻌﺗ ﺎﺑ ي ﺘﻧآ زا ﯽ ﺑﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ي ار ﯾ ﻧﺎﻣرد ﺞ ﯽ ناﻮﺘﺑ ﺎﺗ ،ددﺮﮔ مﺎﺠﻧا
ﺑرﺎﮐ و ﺐﺳﺎﻨﻣ زود دﺮ
ي ﭘ ﺲﻧﺎﺳا ﯿ رد نآ زا و هﺪﺷ ﺺﺨﺸﻣ زﺎ
زﺎﺳوراد و اﺬﻏ ﺖﻌﻨﺻ ي
ﺖﻓﺮﮔ هﺮﻬﺑ .
5 - ﻊﺑﺎﻨﻣ
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Identification of chemical compounds of Allium cepaessential oil and evaluation of its antimicrobial activity on Staphylococcus
aureus, Listeria innocua, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Candida albicans
Bakhshi-Khaniki, Gh.1, Nasehi, B. 2*, Ebrahimi, M. A. 3, Movahedi Saber, N. 4, Alizadeh Behbahani, B.5
1. Department of Agricultural Biotechnology, Payame Noor University (PNU), Iran.
2. Department of Agricultural Engineering and Technology, Payame Noor University (PNU), Iran.
3. Department of Agricultural Biotechnology, Payame Noor University (PNU), Iran.
4. M.Sc. graduate, Department of Agricultural Biotechnology, Payame Noor University (PNU), Iran.
5. Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
ABSTRACT ARTICLE INFO
Article History:
Received 2022/ 01/ 15 Accepted 2022/ 02/ 13
Keywords:
Onion essential oil,
Minimum inhibitory concentration, Micro-dilution broth,
Gas chromatography.
In order to reduce the economic losses and life risks caused by microbial pathogens, the use of natural substances as antimicrobial compounds seems to be an effective way to control the presence of pathogenic bacteria. In this study, the chemical composition of onion essential oil was determined by gas chromatography–mass spectrometry (GC-MS). The methods in disk diffusion agar (DDA) and well diffusion agar (WDA) was used to investigate the inhibition zone diameter, micro-dilution broth was used to measure the minimum inhibitory concentration (MIC) and pour-plate technique was also used for minimum bactericidal/fungicidal (MBC/MFC) concentration. A total of 20 chemical volatiles (approximately 100% of onion essential oil compounds) were identified. The compounds Trisulfide dipropyl, Disulfide dipropyl, n-Decane and Furfuryl methyl sulfide with 20.67, 16.18, 13.51 and 10.23% of the main constituents of onion essential oil, respectively. The results of WDA showed that onion essential oil had the most and the least effect on Gram-positive bacteria Staphylococcus aureus with a diameter of 15 mm and Gram-negative bacteria Pseudomonas aeruginosa with a diameter of 10 mm, respectively. The results of MIC onion essential oil for Staphylococcus aureus, Listeria innocua, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Candida albicans512, 512, 512, 512, 512 and 1024 mg/mL, respectively.
In general, the results of this study showed that the antimicrobial effect of onion essential oil can be somewhat different depending on the type of microorganism.
DOI: 10.52547/fsct.19.123.225
DOR: 20.1001.1.20088787.1401.19.123.30.5
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Iranian Journal of Food Science and Technology
Homepage:www.fsct.modares.ir Scientific Research
