ﺮان ﯾا ﯽﯾ ﻏﺬا ﻊﯾ ﺻﻨﺎ و ﻋﻠﻮم ﻣﺠﻠﻪ

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ﺛﺎﺗ ﯿ ﭘ ﺮ ﯿ ﺶ ﺗﯿ رﺎﻤ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﯾ

ﺮﺑ ﻢ ﺻﻮﺼﺧ ﯿ ﻓ تﺎ ﯿﺰ ﯾﮑ ﯽ ﺷ ﯿﻤ ﯿﺎ ﯾ ﯽ ﮐﺮﺗ و ﯿ ز تﺎﺒ ﯾ ﺖﺴ ﺎﻌﻓ ل ﻪﻧاﻮﺟ مﺪﻨﮔ هدز

ﺎﺷﺎﭘ ﷲا حور ﯾ

ﯽ ماﺮﻬﺑ ﺸﻓا ،1

ﯿ داﻮﺟ ﻦ

2ي كﺪﺑﺪﺑ ﺪﻤﺻ ،*

ﺳ ،3

ﯿ ﻔﻄﺼﻣ ﺪ ﯽ اﺪﺧ جﺮﻋا ﯾ

4ﯽ

1 - ﻮﺠﺸﻧاد ي ﺮﺘﮐد ي ﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ، ﯾ

اﺬﻏ ﻊ ﯾ ﯽ ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،نﺎﻘﻤﻣ ﺪﺣاو ، ﯽ

ا ،نﺎﻘﻤﻣ ، ﯾ ناﺮ .

2 - اﺬﻏ داﻮﻣ ﺖﺷاﺪﻬﺑ هوﺮﮔ ،دﺎﺘﺳا ﯾ

ﯽ ﮑﺷﺰﭙﻣاد هﺪﮑﺸﻧاد ، ﯽ

ﮑﺷﺰﭘ مﻮﻠﻋ ، ﯽ

ﺮﺒﺗ ﯾ ﺎﮕﺸﻧاد ،ﺰ ﻣﻼﺳا دازآ ه ﯽ

ﺮﺒﺗ ، ﯾ ا ،ﺰ ﯾ ناﺮ .

3 - دﺎﺘﺳا ﯾ ﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ،رﺎ ﯾ

اﺬﻏ ﻊ ﯾ ﯽ زروﺎﺸﮐ هﺪﮑﺸﻧاد ، ي

ﺒﻃ ﻊﺑﺎﻨﻣ و ﯿﻌ

ﯽ ﺮﺒﺗ هﺎﮕﺸﻧاد ،ﺮﻫا ﯾ

ﺮﺒﺗ ،ﺰ ﯾ ا ،ﺰ ﯾ ناﺮ .

4 - دﺎﺘﺳا ﯾ ﺘﻨﺳ ﺐﻃ هﺪﮑﺸﻧاد ،رﺎ ﯽ

ﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ، ﯽ

ﺮﺒﺗ ﯾ ﺮﺒﺗ ،ﺰ ﯾ ا ،ﺰ ﯾ ناﺮ .

تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ

ﮑﭼ

ﯿ هﺪ

رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :

رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 27 / 01 / 1401

رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 04 / 03 / 1401

ﻤﻠﮐ تﺎ ﻠﮐ ﯿ ﺪ ي :

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﯾ

،ﻢ

ﻣآ ﺎﻣﺎﮔ ﯿ ﺮﺗﻮﺑﻮﻨ ﯾ ﺳا ﮏ ﯿ ،ﺪ

ﻪﻧاﻮﺟ مﺪﻨﮔ ،هدز

ﻪﻧاﻮﺟ ﻧز ﯽ .

DOI: 10.22034/FSCT.19.128.83

DOR: 20.1001.1.20088787.1401.19.128.17.2

لﻮﺌﺴﻣ

تﺎﺒﺗﺎﮑﻣ : [email protected]

ا فﺪﻫ ﯾ ﻘﺤﺗ ﻦ ﯿ ﺳرﺮﺑ ﻖ ﯽ ﺛﺎﺗ ﯿ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ندوﺰﻓا ﺮ ﯾ

ﻪﺑ ﻢ ﻣ ﺮﺑ اﺮﺘﺴﺑﻮﺳ ناﻮﻨﻋ ﯿ

ﻟﻮﺗ ناﺰ ﯿ ﺪ

ﻣآ ﺎﻣﺎﮔ ﯿ ﺗﻮﺑﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﻃ رد ﺪ ﯽ ﻪﻧاﻮﺟ ﻧز ﯽ دﻮﺑ مﺪﻨﮔ ﻪﻧاد .

اﺮﺑ ي ا ﯾ ﻪﻧاد رﻮﻈﻨﻣ ﻦ ﺎﻫ

ي مﺪﻨﮔ

دﺎﻘﻣ ﺎﺑ ﺪﻧﻮﻟا ﻢﻗر ﯾ

ﺎﻣﺎﺗﻮﻠﮔ ﻒﻠﺘﺨﻣ ﺮ ﺪﺳ ت

ﯾ ﻢ ) mg kg^-1 150

و 100 ، 50 ، 0 ( ﻃ رد ﯽ 72h

ﻪﻧاﻮﺟ ﻧز ﯽ ﺗ ﯿ ﺪﺷ رﺎﻤ . وﯾ ﮔﮋ ﯽ ﺎﻫ ي ﻓ ﯿﺰ ﯾ ﮑ ﯽ ) ﻪﻧاﻮﺟ ﻪﻧاد نزو ﺪﻨﻧﺎﻣ ﻪﻧاﻮﺟ ﻪﻧاد داﺪﻌﺗ ،هدز

و هدز

ﻪﻧاﻮﺟ لﻮﻃ (

ﻧ و ﯿ دﺎﻘﻣ ﺰ ﯾ ﮐﺮﺗ ﺮ ﯿ ز تﺎﺒ ﯾ لﺎﻌﻓ ﺖﺴ )

ﻣآ ﺎﻣﺎﮔ ﺪﻨﻧﺎﻣ ﯿ

ﺗﻮﺑﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺋﻮﻧوﻼﻓ ،ﺪ ﯿ ﺪ

ﻟﺎﻌﻓ و ﻞﮐ لﻮﻨﻓ ،ﻞﮐ ﯿ

ﺸﺑور ﺖ ﯽ دار ﯾ لﺎﮑ ﺎﻫ ي ازآ د DPPH ﺎﻫزﺎﺑ رد

ي ﻧﺎﻣز ﯽ 24h ، 48 و

72 هزاﺪﻧا ﮔﯿ ﺮ ي ﺪﺷ . ﺎﺘﻧ ﯾ اﺰﻓا ﺎﺑ داد نﺎﺸﻧ ﺞ ﯾ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﺶ ﯾ

ﻪﻧاﻮﺟ نﺎﻣز و ﻢ ﻧز

ﯽ ،

ﻪﻧاﻮﺟ ﻪﻧاد نزو ﻪﻧاﻮﺟ ﻪﻧاد داﺪﻌﺗ و هدز

اﺰﻓا هدز ﯾ ﺶ ﯾ ﺖﻓﺎ . ﻨﭽﻤﻫ ﯿ راﺪﻘﻣ نﺎﻣز ﺖﺷﺬﮔ ﺎﺑ ﻦ

ﺋﻮﻧوﻼﻓ ﯿ ﻪﻧﻮﻤﻧ ﻪﻤﻫ رد ﻞﮐ ﺪ ﺶﻫﺎﮐ ﺎﻫ

ﯾ اﺰﻓا و ﺖﻓﺎ ﯾ

ﻣﺎﺗﻮﻠﮔ ﺶ ﯿ ﺳا ﮏ ﯿ ﻧ ﺪ ﯿ ﺛﺎﺗ ﺰ ﯿ ﻨﻌﻣ ﺮ ﯽ راد ي

ﺖﺷاﺪﻧ نآ راﺪﻘﻣ ﺮﺑ .

ﺑﯿ ﺮﺘﺸ ﯾ ﻣآ ﺎﻣﺎﮔ ،ﻞﮐ لﻮﻨﻓ راﺪﻘﻣ ﻦ ﯿ

ﺗﻮﺑﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﻓﺮﻇ و ﺪ ﯿ ﺖ

ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻪﻧﻮﻤﻧ رد ﺗﯿ

هﺪﺷ رﺎﻤ mg kg^-1 150

ﻪﻧاﻮﺟ لوا زور رد ﻧز

ﯽ ﺗﺮﺗ ﻪﺑ ﯿ ﺮﺑاﺮﺑ ﺐ

ﺎﺑ μg GAE g^-1 4589

، mg 100 g^-1 303

و 57 % ﺪﺷ هﺪﻫﺎﺸﻣ .

ﺗ ﻪﻤﻫ رد ﯿ ﻃ رد ﺎﻫرﺎﻤ ﯽ

ﻪﻧاﻮﺟ نﺎﻣز ﻧز

ﯽ ا راﺪﻘﻣ ﯾ ﮐﺮﺗ ﻦ ﯿ ﻐﺗ تﺎﺒ ﯿﯿ ﻨﻌﻣ ﺮ ﯽ راد ي ﺖﺷاﺪﻧ ) 05 / 0

>

.(p ﺎﻬﻧ رد ﯾ ﺖ

ﺘﻧﯿ ﻪﺠ ﮔﯿ ﺮ ي ﻪﻧاﻮﺟ نﺎﻣز ﻪﮐ ﺪﺷ ﻧز

ﯽ 24 h راﺪﻘﻣ و mg kg^-1

150 ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﯾ

ﻢ

ﺐﺳﺎﻨﻣ ﺮﺗ ﯾ ﺗ ﻦ ﯿ اﺮﺑ رﺎﻤ ي اﺰﻓا ﯾ ﺑ ﺶ ﯿ ﻣآﺎﻣﺎﮔ ﺰﺘﻨﺳﻮ ﯿ

ﺮﺗﻮﺑﻮﻨ ﯾ ﺳا ﮏ ﯿ ﻪﻧاد رد ﺪ ﺎﻫ ي ﻪﻧاﻮﺟ مﺪﻨﮔ هدز

دﻮﺑ .

ﻪﻠﺠﻣ

مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ

www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ

ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ

(2)

1 - ﻪﻣﺪﻘﻣ

مﺪﻨﮔ ) Triticumaestivum (

ﺪﻗ زا ﯾﻤ ﯽ ﺮﺗ ﯾ فﺮﺼﻣﺮﭘ و ﻦ ﺮﺗ

ﯾ ﻦ

ﮔﯿ ﻋارز نﺎﻫﺎ ﯽ ﻣ نﺎﻬﺟ ﯽ ﺪﺷﺎﺑ . ﻤﻫا ﯿ ﺑ مﺪﻨﮔ ﺖ ﯿ

ﻪﺑ طﻮﺑﺮﻣ ﺮﺘﺸ

ﻓ صاﻮﺧ ﯿﺰ ﯾﮑ ﯽ ﺷ ﯿﻤ ﯿﺎﯾ ﯽ ﮐﺮﺗ و ﯿ ﮑﺸﺗ تﺎﺒ ﯿ نآ هﺪﻨﻫد ﻞ

ﺖﺳا ﺌﺗوﺮﭘ. ﯿ ﺬﻐﺗ ظﺎﺤﻟ زا مﺪﻨﮔ ﻦ ﯾﻪ

ا ي ژﻮﻟﻮﻨﮑﺗ و ﯾﮑ

ﯽ ﺰﺋﺎﺣ

ﻤﻫا ﯿ ﺖﺳا ﺖ . ﻢﻫ ﻨﭼ ﯿ اراد مﺪﻨﮔ سﻮﺒﺳ ﻦ ي

ﺌﺗوﺮﭘ ﯿ و ﻦ

وﯾ ﻣﺎﺘ ﯿ ﻦ ﺎﻫ ي ﻔﻠﺘﺨﻣ ﯽ ﺪﻨﻧﺎﻣ ،A ،B و C ﻣE ﯽ ﺪﺷﺎﺑ . اراد مﺪﻨﮔ ي

ﻠﮐ ﺶﺨﺑ ﻪﺳ ﯽ

ﻤﻬﻧاﻮﺟ و ﻪﺘﺳﻮﭘ ،مﺮﭙﺳوﺪﻧآ ﯽ

ﺪﺷﺎﺑ . زا هدﺎﻔﺘﺳا

ﻪﻧاﻮﺟ ﻧز ﯽ ﺷور ناﻮﻨﻋ ﻪﺑ هﺪﺷ لﺮﺘﻨﮐ ﯽ

اﺮﺑ ي شزرا دﻮﺒﻬﺑ

ﺬﻐﺗ ﯾﻪ ا ي ﺖﺳا ﻪﺟﻮﺗ درﻮﻣ تﻼﻏ تﻻﻮﺼﺤﻣ ﻢﻌﻃ و .

اﺰﻓا ﯾ ﺶ

ﻟﺎﻌﻓ ﯿ ﺰﻧآ ﺖ ﯾﻤ ﯽ ﻃ رد ﯽ ﻪﻧاﻮﺟ ﻧز ﯽ ﺛﺎﺗ ﯿﺮ ﺑﺮﺨﻣ ﯽ ﮐ ﺮﺑ ﯿﻔ ﯿ اﻮﻧﺎﻧ ﺖ ﯾ ﯽ

زﺎﺳدرآ و ي دراد مﺪﻨﮔ . آﺮﻓ ﯾ ﻪﻧاﻮﺟ ﺪﻨ ﻧز ﯽ ﮐ ﺮﺑ ﯿﻔ ﯿ اﻮﻧﺎﻧ ﺖ ﯾ ﯽ درآ

ﺛﺎﺗ ﯿ دراد ﺮ . ﺎﺑ ﺎﻣا ﯾ ﻪﻧاﻮﺟ ﺪ ﻧز ﯽ ﻪﺑ ار ناﻮﻨﻋ ﯾ اﺮﺑ ﺐﺳﺎﻨﻣ شور ﮏ ي

ﺬﻐﺗ شزرا دﻮﺒﻬﺑ ﯾﻪ

ا ي ﺖﻓﺮﮔ ﺮﻈﻧ رد مﺪﻨﮔ 1]

. ا[ ﯾ ﻟد ﻪﺑ ﺮﻣا ﻦ ﯿ

ﻞ

اﺰﻓا ﯾ ﻠﺑﺎﻗ ﺶ ﯿ اﺰﻓا و ﻪﺘﺳﺎﺸﻧ ﻢﻀﻫ ﺖ ﯾ

ز ﺶ ﯾ ﺳﺮﺘﺳد ﺖﺴ ﯽ

ﻣآ ﯿ ﻮﻨ

ﺳا ﯿ ﮑﺸﺗ و ﺎﻫﺪ ﯿ

ﺪﻨﭼ ﻞ ﯾ ﮐﺮﺗ ﻦ ﯿ ز ﺐ ﯾ ﻃ رد لﺎﻌﻓ ﺖﺴ ﯽ

ﻪﻧاﻮﺟ ﻧز ﯽ

ﺖﺳا . اﺰﻓا تﺎﻌﻟﺎﻄﻣ ﯾ

ﺎﻫﺪﻨﻗ رد ﺶ ي

ﺣا ﯿ ﻣآ و ءﺎ ﯿ ﺳا ﻮﻨ ﯿ ﺎﻫﺪ ي ،دازآ

ﻣآ ﺎﻣﺎﮔ ﻪﻠﻤﺟ زا ﯿ

ﺗﻮﺑ ﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪ 2] ﻧﺪﻌﻣ داﻮﻣ [ ﯽ ﻞﺑﺎﻗ

ﺳﺮﺘﺳد ﯽ ز ﯾ ﺘﺴ ﯽ 3] ﻓ ،[ ﯿ ﺎﻫﺮﺒ ي ژر ﯾﻤ ﯽ ﮐﺮﺗ ،لﻮﻠﺤﻣ ﯿ

ﻟﻮﻨﻓ تﺎﺒ ﯽ و

ﺻﺎﺧ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ 4] ﻃ رد ار [ ﯽ ﻪﻧاﻮﺟ د ندز نﺎﺸﻧ ﻪﻧا

ﻣ ﯽ ﺪﻫد . ﺑ رد ﯿ ﮐﺮﺗ ﻦ ﯿ اﺰﻓا تﺎﺒ ﯾ

ﺶ ﯾ ﻃ رد ﻪﺘﻓﺎ ﯽ

ﻪﻧاﻮﺟ ﻧز ﯽ

، ﻪﺑ GABA ناﻮﻨﻋ ﯾ ﮐﺮﺗ ﮏ ﯿ ءﺎﻘﺗرا رد ﺮﺛﻮﻣ ﺐ

ﺑ ﺖﻣﻼﺳ ﯿ ﺖﺳا ﻪﺟﻮﺗ درﻮﻣ ﺮﺘﺸ .

ﯾGABA ﮏ ﻣآ ﯿ ﺳا ﻮﻨ ﯿ ﺪ

4 ﻏ و ﻪﻨﺑﺮﮐ ﯿ ﺗوﺮﭘﺮ ﯿﻨﺌ ﯽ ﻠﺻا ءﺰﺟ ﻪﮐ ﺖﺳا ﯽ

ﻣآ ﯿ ﺳا ﻮﻨ ﯿ ﺎﻫﺪ رد دازآ

لﻮﻠﺳ ﺮﺜﮐا ﺎﻫ

ي ﮐوﺮﭘ هﺪﻧز تادﻮﺟﻮﻣ رﺎ

ﯾ و تﻮ ﯾ رﺎﮐﻮ ﯾ تﻮ

ﻣ ﯽ ﺪﺷﺎﺑ 5] .[ ﯾGABA ﮑ ﯽ ﺮﺘﻤﻬﻣ زا ﯾ ﻤﺴﻧاﺮﺗورﻮﻧ ﻦ ﯿ

ﺎﻫﺮﺘ ي

رﺎﻬﻣ ي ﺳ رد ﯿ ﺒﺼﻋ ﻢﺘﺴ ﯽ ﺰﮐﺮﻣ ي هﺮﻬﻣ فﺮﻃ زا ،ﺖﺳا ناراد

دﯾ اراد ﺮﮕ ي ﺪﻨﭼ ﯾ ﻓ دﺮﮑﻠﻤﻋ ﻦ ﯿﺰ

ﯾ ژﻮﻟﻮ ﯾﮑ ﯽ ﺶﻫﺎﮐ ءﺎﻘﻟا ﻪﻠﻤﺟ زا

ﮑﺴﺗ و روآراردا تاﺮﺛا ،نﻮﺧرﺎﺸﻓ ﯿ

ﮔﺪﻨﻫد ﻦ ﯽ ﮔﻮﻠﺟ ، ﯿﺮ ي زا

ﺜﮑﺗ ﯿ لﻮﻠﺳ ﺮ ﺎﻫ ي ﻧﺎﻃﺮﺳ ﯽ ﺛا ، ور سﺮﺘﺳا ﺪﺿ تاﺮ ي

ﺖﻣﻼﺳ

نﺎﺴﻧا ﻣ ﯽ و ﺪﺷﺎﺑ ﯾ ﻮﻗ كﺮﺤﻣ ﮏ ي

اﺮﺑ ي ﻟﻮﺴﻧا ﺢﺷﺮﺗ ﯿ

زا ﻦ

ﺖﺳا ساﺮﮑﻧﺎﭘ .

ﻨﭽﻤﻫ ﯿ ﻣ ﻦ ﯽ ﺶﻘﻧ ﺪﻧاﻮﺗ ﯾ

ﻈﻨﺗ ﮏ ﯿ ﻢ هﺪﻨﻨﮐ

ﺬﻐﺗ ﯾﻪ ا ي ور ي ا ار ساﺮﮑﻧﺎﭘ ﯾ

ﺮﺛﻮﻣ رﻮﻃ ﻪﺑ و ﺪﻨﮐ ءﺎﻔ ي

اﺮﺷ زا ﯾ ﻂ

دﯾ ﺘﺑﺎ ﯿ ﮔﻮﻠﺟ ﮏ ﯿﺮ ي ﺪﻨﮐ . ﻘﺤﺗ ﯿ ﺧا تﺎﻘ ﯿ ﺖﺳا هداد نﺎﺸﻧ ﺮ

ﻣآﺎﻣﺎﮔ ﯿ ﺗﻮﺑ ﻮﻨ ﯿﺮ ﯾ ﮏ ﺳا ﯿ ﺎﻫ نﻮﻣرﻮﻫ ﺖﻈﻠﻏ ﺖﺳا ﻦﮑﻤﻣ ﺪ ي

ﺪﺷر

ﺗوﺮﭘ ﺰﺘﻨﺳ ﺖﻋﺮﺳ و ﺎﻤﺳﻼﭘ ﯿ

ﺪﺸﺨﺑ دﻮﺒﻬﺑ ار ﺰﻐﻣ رد ﻦﺌ 6]

. [

ﯾ نارﺎﮑﻤﻫ و ﮓﻧﺎ )

2014 ( ﺛﺎﺗ ، ﯿ ور ﺮﺑ ار ﺪﻧﻮﺳاﺮﺘﻟوا ﺮ ي

دﻮﺒﻬﺑ ﮐارﻮﺧ صاﻮﺧ

ﯽ ﺬﻐﺗ و ﯾﻪ ا ي ﻨﭽﻤﻫ و ﯿ ﮐ ﻦ ﯿﻔ ﯿ ﻪﻧاﻮﺟ ﺖ ﺎﻫ ي ﻪﻧاد

ﻮﺳ ﯾ ﺳرﺮﺑ درﻮﻣ ﺎ ﯽ

ﺪﻧداد راﺮﻗ .

ﻪﻧاد ﺎﻬﻧآ ﺎﻫ ي ﻮﺳ ﯾ ﺖﺤﺗ ار ﺎ

ناﻮﺗ ﺎﻫ ي ﺪﻧﻮﺳاﺮﺘﻟوا ﻒﻠﺘﺨﻣ )

0 ﺎﺗ 300 تاو ( ﺗﯿ و ﺪﻧدﻮﻤﻧ رﺎﻤ

رد ﯾ ﺗ ﻪﮐ ﺪﻨﺘﻓﺎ ﯿ ﻪﻧاﻮﺟ ﺖﻋﺮﺳ ﺪﻧﻮﺳاﺮﺘﻟوا رﺎﻤ ﻧز

ﯽ و ﻪﻧاﻮﺟ لﻮﻃ و

ﻣﯿ ﺗﻮﺑﺎﻣﺎﮔ ناﺰ ﯿﺮ

ﯾ ﺳا ﮏ ﯿ اﺰﻓا ار ﺪ ﯾ

ﻣ ﺶ ﯽ ﺪﻫد 7] .[ ﺎﮐوﺎﮔﺎﻧ

) 2005 ( ﺗ ، ﯿ ﺎﻫرﺎﻤ ي اﺰﻓا ﺖﻬﺟ ار ﺖﻬﺟ ﻒﻠﺘﺨﻣ ﯾ

حﻮﻄﺳ ﺶ

ﺗﻮﺑﺎﻣﺎﮔ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺳا و ﺪ ﯿ ﻓ ﺪ ﯿﺘﯿ ﻟﺎﺗﺎﮐ ﮏ ﯿ ﺰﻧآ ﻂﺳﻮﺗ هﺪﺷ ﺰ ﯾﻢ

ﺎﻫ ي

ﻧورد ﯽ ﺪﻧداد راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ ار مﺪﻨﮔ ﻪﻧاد .

رد ﺎﻬﻧآ ﯾ ﻪﮐ ﺪﻨﺘﻓﺎ

ﮐﺮﺗ حﻮﻄﺳ ﯿ ز تﺎﺒ ﯾ ﻣ مﺪﻨﮔ لﺎﻌﻓ ﺖﺴ ﯽ

ﮕﻤﺸﭼ رﻮﻃ ﻪﺑ ﺪﻧاﻮﺗ ﯿﺮ

ي

رﺎﮑﺘﺳد ﺎﺑ ي اﺮﺷ ﯾ ﻟﺎﻌﻓ زا هدﺎﻔﺘﺳا و ندز ﻪﻧاﻮﺟ ﻂ ﯿ

ﺖ ﺰﻧآ ﯾﻢ ﺎﻫ ي

ﻧورد ﯽ اﺰﻓا ﯾ ﺶ ﯾ ﺪﺑﺎ . ﺎﺘﻧ ﯾ ا ﺞ ﯾ ﻫدزﺎﺑ ﻪﮐ داد نﺎﺸﻧ نﺎﻘﻘﺤﻣ ﻦ ﯽ

ﺎﻣﺎﮔ

ﺗﻮﺑ ﯿﺮ ﯾ ﺳا ﮏ ﯿ مﺪﻨﮔ زا ﺪ ﻪﻧاﻮﺟ

هﺪﺸﻧ لﺮﺘﻨﮐ حﻮﻄﺳ رد هدز

ﺴﮐا ﯿ لﻮﻠﺤﻣ نﮋ 3

18± ﺑ رﺎﺑ ﯿ مﺪﻨﮔ زا ﺮﺘﺸ ﺎﻫ

ي ﻞﻤﮑﻣ نوﺪﺑ

ﺴﮐا ﯿ دﻮﺑ نﮋ . ﻨﭽﻤﻫ ﯿ ﺗﻮﺑﺎﻣﺎﮔ ﺖﻈﻠﻏ ﻦ ﯿﺮ

ﯾ ﺳا ﮏ ﯿ ر رد ﺪ ﯾ مﺪﻨﮔ ﻪﺸ

ﺎﺳ زا ﯾ ﺑ نآ طﺎﻘﻧ ﺮ ﯿ

دﻮﺑ ﺮﺘﺸ 8] .[

ﻟﺰﻧاﺮﺑ ﯿﻠﻨ ﯽ نارﺎﮑﻤﻫ و )

2018 ( ﻐﺗ ، ﯿ ﻟﺎﻌﻓ رد تاﺮ ﯿ

ﺰﻧآ ﺖ ﯾﻤ ﯽ ،

ﮐﯿ ﻔﯿ ژﻮﻟﻮﻨﮑﺗ ﺖ ﯾﮑ

ﯽ اﻮﺘﺤﻣ و ي ﻣآﺎﻣﺎﮔ ﯿ ﺗﻮﺑﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿﺪ

) (GABA ﻪﻧاﻮﺟ مﺪﻨﮔ درآ رد ﺳرﺮﺑ درﻮﻣ ار هﺪﺷ هدز

ﯽ راﺮﻗ

ﺪﻧداد . ﻪﻧاﻮﺟ تاﺮﺛا ﺎﻬﻧآ ﻧز

ﯽ ﻪﻋرﺰﻣ زا ﺖﺷادﺮﺑ زا ﻞﺒﻗ مﺪﻨﮔ

) (PHS اﺮﺷ و ﯾ ﻪﻧاﻮﺟ ﻂ ﻧز ﯽ ﺎﻣزآ رد هﺪﺷ ﺎﻘﻟا ﯾ

تﺪﻣ ﻪﺑ هﺎﮕﺸ h

24 ، 8 و 72 ﺳرﺮﺑ درﻮﻣ ار ﯽ

ﺪﻧداد راﺮﻗ . ﺎﺘﻧ ﯾ ﮐﺎﺣ ﺞ ﯽ ا زا ﯾ دﻮﺑ ﻦ

ﻪﻧاﻮﺟ ﻪﮐ ﻧز ﯽ ﻗ ﺶﻫﺎﮐ ﺐﺟﻮﻣ ﻌﻀﺗ و ﻦﺗﻮﻠﮔ ترﺪ

ﯿ ﺌﺗوﺮﭘ ﻒ ﯿ ﻦ

ﻣ ﯽ ﻟو ددﺮﮔ ﯽ ا ﺎﺑ ﯾ اﺰﻓا ﺐﺒﺳ لﺎﺣ ﻦ ﯾ

ﻣ و نﺎﻧ ﻢﺠﺣ ﺶ ﯿ

ناﺰ

ﺗﻮﺑﺎﻣﺎﮔ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪﺷ ﺪ 9] . [

دﯾ نارﺎﮑﻤﻫ و ﮓﻨ )

2018 ( ﺛﺎﺗ ، ﯿ ﻪﻧاﻮﺟ ﺮ ﻧز ﯽ ور ﺮﺑ هﺪﺷ لﺮﺘﻨﮐ ي

ﺻﻮﺼﺧ ﯿ ﻓ تﺎ ﯿﺰ ﯾﮑ ﯽ ﺷو ﯿﻤ ﯿﺎﯾ ﯽ دﺮﺑرﺎﮐ صاﻮﺧ و ي

مﺪﻨﮔ درآ

اﺰﻓا و ﻞﻣﺎﮐ ﯾ

ﻣ ﺶ ﯿ ﺗﻮﺑﺎﻣﺎﮔ ناﺰ ﯿﺮ

ﯾ ﺳا ﮏ ﯿ ﺪﻧﻮﺳاﺮﺘﻟوا ﻂﺳﻮﺗ ﺪ ار

ﺳرﺮﺑ درﻮﻣ ﯽ

ﺪﻧداد راﺮﻗ .

ﺑ ﺎﻬﻧآ ﯿ زا ﺪﻌﺑ ﻪﮐ ﺪﻧدﺮﮐ نﺎ 15h

ﻪﻧاﻮﺟ ﻧز ﯽ ﻣ هﺪﺷ لﺮﺘﻨﮐ ﯿ

ﻟﺎﻓ ناﺰ ﯿ ﻞﺑﺎﻗ رﻮﻃ ﻪﺑ ﺮﺒﻣﺎﻧ ﮓﻨ

ﻪﻈﺣﻼﻣ ا ي ﻣ و ﻪﺘﺷاد ﺶﻫﺎﮐ ﯿ

ﺰﮐﻮﻠﮔ ناﺰ 227

- 357 % اﺰﻓا ﯾ ﺶ

ﯾ ﺖﻓﺎ . ﻨﭽﻤﻫ ﯿ ﻣ ﻦ ﯿ ﻤﺧ رد ﺖﺴﮑﺷ ناﺰ ﯿ

ﻞﻣﺎﮐ مﺪﻨﮔ زا ﻪﻠﺻﺎﺣ ﺮ

ﺶﻫﺎﮐ ﯾ ﺮﮔوﺮﺗر ﻪﺟﻮﺗ ﻞﺑﺎﻗ ﺶﻫﺎﮐ هﺪﻨﻫد نﺎﺸﻧ ﻪﮐ ﺖﻓﺎ ﺳادا

ﯿ نﻮ

رد ﻪﺘﺳﺎﺸﻧ ﯾ

ﺳ ﮏ ﯿ ﺑآ ﻢﺘﺴ ﯽ ﺖﺳا . زا ﺪﻌﺑ 72h ﻪﻧاﻮﺟ ﻧز ﯽ ﻣ ﯿ ناﺰ

ﻣ ﻪﺑ GABA ﯿ ناﺰ 339 % ﺎﻘﻣ رد ﯾ هدﺰﻧ ﻪﻧاﻮﺟ ﻪﻧﻮﻤﻧ ﺎﺑ ﻪﺴ

اﺰﻓا ﯾ ﺖﺷاد ﺶ . ﻨﭽﻤﻫ ﯿ ﻣ ﻦ ﯿ ناﺰ زا ﻞﺻﺎﺣ ﻪﻧﻮﻤﻧ رد GABA

ﻔﺳ مﺮﻧ مﺪﻨﮔ ﯿ

ﺗ ﺖﺤﺗ ﻪﮐ ﺪ ﯿ

ﻪﺑ دﻮﺑ ﻪﺘﻓﺮﮔ راﺮﻗ ﺪﻧﻮﺳاﺮﺘﻟوا رﺎﻤ

ﻣﯿ ناﺰ 7/

30 % ﺑﯿ ﺎﺳ زا ﺮﺘﺸ ﯾ ﻪﻧﻮﻤﻧ ﺮ دﻮﺑ ﺎﻫ 2] . [

(3)

ﺴﮐﻮﺑﺮﮐد زا GABA ﯿ

ﺳﻼ ﯿ نﻮ L ﻣﺎﺗﻮﻠﮔ– ﯿ ﺳا ﮏ ﯿ ﺰﻧآ ﻂﺳﻮﺗ ﺪ ﯾ

ﻢ

ﺴﮐﻮﺑﺮﮐد تﺎﻣﺎﺗﻮﻠﮔ ﯿ

لﻮﻠﺳ رد زﻼ ﺎﻫ

ي ز ﯾ ﺘﺴ ﯽ ﻟﻮﺗ ﯿ ﻣ ﺪ ﯽ دﻮﺷ .

آﺮﻓ ﯾ ﻪﻧاﻮﺟ ﺪﻨ ﻧز ﯽ ﺳا ندوﺰﻓا و ﯿ

ﻣﺎﺗﻮﻠﮔ ﺪ ﯿ ﮏﻤﻧ ترﻮﺻ ﻪﺑ ﮏ

ﭘ ناﻮﻨﻋ ﻪﺑ ﻢ ﯿ

ﺶ زﺎﺳ ﻣ GABA ﯽ ﺴﻣ ﺪﻧاﻮﺗ ﯿ ﺮ

ﻟﻮﺑﺎﺘﻣ ﯿ ﮑ ﯽ ﻟﻮﺗ ﯿ ﺪ ﺮﺤﺗ ار GABA ﯾ ﻟﻮﺗ ﺖﻋﺮﺳ و ﮏ ﯿ

ا ﺪ ﯾ ﻦ هدﺎﻣ

اﺰﻓا ار ﯾ ﺪﻫد ﺶ . ا فﺪﻫ ﯾ ﻦ ﻘﺤﺗ ﯿ ﺳرﺮﺑ ﻖ ﯽ ﻪﻧاﻮﺟ نﺎﻣز ﺮﺛا ﻧز

ﯽ و

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ راﺪﻘﻣ ﯾ

ﻣ ﺮﺑ ﻢ ﯿ ناﺰ ﺎﺳ و GABA

ﯾ ﮐﺮﺗ ﺮ ﯿ تﺎﺒ

زﯾ دﻮﺑ مﺪﻨﮔ ﻪﻧاﻮﺟ لﺎﻌﻓ ﺖﺴ .

2 - ﺎﻫ شور و داﻮﻣ

2 - 1 - هدﺎﻔﺘﺳا درﻮﻣ داﻮﻣ

ا رد ﯾ ﻘﺤﺗ ﻦ ﯿ زروﺎﺸﮐ دﺎﻬﺟ نﺎﻣزﺎﺳ زا ﺪﻧﻮﻟا ﻢﻗر مﺪﻨﮔ ﻖ ي

نﺎﺘﺳا

ﺎﺑرذآ ﯾ ﻗﺮﺷ نﺎﺠ ﯽ ﻬﺗ ﯿ ﺪﺷ ﻪ . ﺎﻣزآ داﻮﻣ ﯾ ﻫﺎﮕﺸ ﯽ ﻟﺎﻧآ ﻪﺟرد ﺎﺑ ﯿﺰ

ي زا

ﺳ و كﺮﻣ ﺖﮐﺮﺷ ﯿ

ﻬﺗ ﺎﻤﮕ ﯿ دﺮﮔ ﻪ ﯾ ﺪﻧﺪ .

2 - 2 - ﻪﻧاﻮﺟ شور ﻧز

ﯽ ﻪﻧاد ﺎﻫ ي ﺗ مﺪﻨﮔ ﯿ هﺪﺷرﺎﻤ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺎﺑ ﯾﻢ

اﺮﺑ ي ا ﯾ ﻪﻧاد اﺪﺘﺑا رﻮﻈﻨﻣ ﻦ ﺎﻫ

ي ﻤﺗ مﺪﻨﮔ ﯿ ﺼﻟﺎﺧﺎﻧ و هﺪﺷ ﺰ ﯽ

زا ﺎﻫ

ﺪﺷ اﺪﺟ نآ .

ﻧﻮﻔﻋﺪﺿ زا ﺲﭙﺳ ﯽ

ﻫ لﻮﻠﺤﻣ ﺎﺑ ﯿ

ﺮﻠﮐﻮﭙ ﯾ ﺪﺳ ﺖ ﯾ ﻢ

1 % اﺮﺑ ي تﺪﻣ 5min و ﺸﮑﺑآ ﯽ ﻪﻧﻮﻤﻧ ،ﺮﻄﻘﻣ بآ ﺎﺑ بآ رد ﺎﻫ

ﺧ ﯿ تﺪﻣ ﻪﺑ و هﺪﺷ هﺪﻧﺎﺴ 24h

ﺎﻣد رد ي 30 °C بآ ﻞﺧاد رد

راﺪﻬﮕﻧ ي ﺪﻧﺪﺷ . ﻪﻧﻮﻤﻧ ﺲﭙﺳ رد ﺎﻫ

ﯾ ﺷ فﺮﻇ ﮏ ﯿ

ﻪﺸ ا ي ﻦﻬﭘ

ﻔﺳ ﻪﭼرﺎﭘ و هﺪﺷ ﯿ

ور ﺪ ي ﺸﮐ نآ ﯿ وﺎﺣ لﻮﻠﺤﻣ و ﺪﺷ هﺪ ي

ﺖﻈﻠﻏ ﺎﻫ ي ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﻒﻠﺘﺨﻣ ﯾ

ﻢ ) mg kg^-1 50

، 100 و

150 ( ﻫﺎﺷ ﻪﻧﻮﻤﻧ هاﺮﻤﻫ ﻪﺑ بآ رد ﺪ

) تﺎﻣﺎﺗﻮﻠﮔ ندوﺰﻓا نوﺪﺑ

ﺪﺳ ﯾﻢ ( ور ﺮﺑ ي ﺮﭙﺳا ﻪﭼرﺎﭘ ي

ﻪﻧﻮﻤﻧ و ﺪﺷ ﺎﻫ

ي ﺗ ﯿ رد هﺪﺷرﺎﻤ

ﺎﻣد ي 30°C تﺪﻣ ﻪﺑ 3 ﻪﻧﺎﺨﻣﺮﮔ زور راﺬﮔ

ي ﺪﻧﺪﺷ . ﺎﻫزور رد ي

ﻪﻧﻮﻤﻧ مﻮﺳ و مود ،لوا رادﺮﺑ

ي ﻪﻧﻮﻤﻧ و هﺪﺷ مﺎﺠﻧا ﺎﻣد رد ﺎﻫ

ي

60°C تﺪﻣ ﻪﺑ نوآ رد 3h

ﻪﺘﺴﺑرد فوﺮﻇ رد و ﺪﻧﺪﺷ ﮏﺸﺧ

ﺎﻣد رد ي 18 °C - راﺪﻬﮕﻧ ي ﺪﻧﺪﺷ . اﺮﺑ ي ﺎﻣزآ مﺎﺠﻧا ﯾ

تﺎﺸ

ﻪﻧﻮﻤﻧ ،ﻪﻃﻮﺑﺮﻣ ﺳآ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ

ﯿ ﮕﻧﺎﺧ بﺎ ﯽ ﺳآ ﯿ ﺪﻧﺪﺷ بﺎ .

2 - 3 - ﺎﻣزآ ﯾ ﻓ تﺎﺸ ﯿﺰ

ﯾ ﮑ ﯽ - ﺷ ﯿﻤ ﯿﺎ ﯾ ﯽ ﻪﻧاد ﺎﻫ ي

ﻪﻧاﻮﺟ مﺪﻨﮔ هدز

2 - 3 - 1 - ﻧز ﻪﻧاﻮﺟ ﺪﺻرد ﯽ

ﻧز ﻪﻧاﻮﺟ ﺪﺻرد ﯽ

ﻪﻧاﻮﺟ روﺬﺑ داﺪﻌﺗ ﺖﺒﺴﻧ زا ﻞﮐ داﺪﻌﺗ ﻪﺑ هدز

ﺪﻣآ ﺖﺳﺪﺑ روﺬﺑ .

2 - 3 - 2 - ﻪﻧاﻮﺟ لﻮﻃ

ﻪﻧاﻮﺟ لﻮﻃ هزاﺪﻧا ﺶﮐ ﻂﺧ زا هدﺎﻔﺘﺳا ﺎﺑ

ﮔﯿ ﺮ ي ﺐﺴﺣ ﺮﺑ و

ﺪﺷ شراﺰﮔ cm .

2 - 3 - 3 - ﻪﻧاﻮﺟ ﺮﺗ نزو

اﺮﺑ ي هزاﺪﻧا ﮔﯿ ﺮ ي ر اﺪﺘﺑا ﻪﻧاﻮﺟ ﺮﺗ نزو ﯾ

ﻪﺸ ﻪﻧاﻮﺟ و فﺬﺣﺎﻫ

نورد ﺖﮐﺎﭘ ﺖﻓﺮﮔ راﺮﻗ وزاﺮﺗ ﺎﺑ ﺲﭙﺳ و

ي د ﯾ ﺠ ﯿ ﻟﺎﺘ ﯽ ﺖﻗد ﺎﺑ g

001 /0 زﻮﺗ ﯾ ﺪﺷ ﻦ .

2 - 3 - 4 - راﺪﻘﻣ GABA

راﺪﻘﻣ ﻟ شور ﻂﺳﻮﺗ GABA

ﯿ نارﺎﮑﻤﻫ و ﻮ )

2013 (

هزاﺪﻧا ﮔﯿ ﺮ ي ﺪﺷ 10] .[ اﺮﺑ ي ا ﯾ رﻮﻈﻨﻣ ﻦ 5g

/0 ﻪﻧاد ردﻮﭘ

ﻪﻧاﻮﺟ ﺎﺑ هﺪﺷ ﮏﺸﺧ هﺪﺷ هدز 5 mL

لﻮﻠﺤﻣ 10 %

ﺮﺗ ي ﺘﺳاوﺮﻠﮐ ﯿ ﺳا ﮏ ﯿ تﺪﻣ ﻪﺑ و طﻮﻠﺨﻣ ﺪ 1min

و هﺪﺷ هدﺰﻤﻫ

تﺪﻣ ﻪﺑ ﺲﭙﺳ 2h

رد 40 °C جاﺮﺨﺘﺳا ﺖﻬﺟ GABA

راﺪﻬﮕﻧ ي ﻧﺎﺳ هﺎﮕﺘﺳد زا هدﺎﻔﺘﺳا ﺎﺑ و ﺪﺷ ﺮﺘ

ﯾﻔ ﯿ ﺎﺑ ژﻮ 10000rpm

تﺪﻣ ﻪﺑ 15min

ﺮﺘﻧﺎﺳ ﯾﻔ ﯿ ﺎﻣ زﺎﻓ و ﺪﺷ ژﻮ ﯾ

ﻻﺎﺑ ﻊ ﯾ ﯽ زا هدﺎﻔﺘﺳا ﺎﺑ

ﻓﯿ ﺮﺘﻠ 45 mμ /0 ﺪﺷ فﺎﺻ .

ﺖﻈﻠﻏ هرﺎﺼﻋ رد GABA

ﺎﻫ ي

هﺎﮕﺘﺳد ﻂﺳﻮﺗ هﺪﺷ جاﺮﺨﺘﺳا ) HPLC

ﺷ ﯿ ﻦﭘاژ ،وزدﺎﻤ (

و ﺪﻌﺑ

ﻖﺘﺸﻣ زﺎﺳ ي د لﺎﺘﻓٌا ﺎﺑ ي ﺋﺪﻟآ ﯿ ﻟﺎﻧآ ﺪ ﯿ ﺪﺷ ﺰ . ﺮﻓﺎﺑ ود زا كﺮﺤﺘﻣ زﺎﻓ

) A ﺪﺳ تﺎﺘﺳا ﯾﻢ 02M /0 ، ﻈﻨﺗ pH ﯿ ور هﺪﺷ ﻢ ي 3 /7 ﺳا ﺎﺑ ﯿ ﺪ

ﺘﺳا ﯿ

،ﮏ Lμ 200 ﺮﺗ ي ﺗا ﯿ ﻣآ ﻞ ﯿ ﺮﻫ رد ﻦ 1000mL

لﻮﻠﺤﻣ ( و

ﺮﻓﺎﺑ ) B ﺳا ﯿ ﻧﻮﺘ ﯿ ﺮﺘ ﯾ ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ ﻪﺟرد ﺎﺑ ﻞ ﯽ

( ﮑﺸﺗ ﯿ دﻮﺑ هﺪﺷ ﻞ .

80Lμ ﻪﻧﻮﻤﻧ هرﺎﺼﻋ ﺎﺑ ﺎﻫ

Lμ 400 لﻮﻠﺤﻣ زا 4M

/0 ﺮﻓﺎﺑ

تارﻮﺑ ﮏﻤﻧ )

ﻈﻨﺗ pH ﯿ ور هﺪﺷ ﻢ ي 4 / 10 لﻮﻠﺤﻣ زا هدﺎﻔﺘﺳا ﺎﺑ

ﻫﯿ ﺴﮐورﺪ ﯿ ﺪﺳﺪ ﯾ ﻢ 40 (%

و 80 Lμ ﻖﺘﺸﻣ ﺮﮕﺸﻨﮐاو زﺎﺳ

ي ) ﺎﺑ

ندﺮﮐ ﻞﺣ 10 mg

د لﺎﺘﻓٌا ي ﺋﺪﻟآ ﯿ رد ﺪ 20Lμ

زا

2 - رد لﻮﻧﺎﺗاﻮﺘﭘﺎﮐﺮﻣ 5mL

/2 ﺳا ﯿ ﻧﻮﺘ ﯿ ﺮﺘ ﯾ ﻞ ( ﺎﻣد رد ي ﻪﺑ قﺎﺗا

تﺪﻣ 5min ﺪﺷ طﻮﻠﺨﻣ ﺶﻨﮐاو مﺎﺠﻧا ﺖﻬﺟ .

20 Lμ زا

ﻖﺘﺸﻣ لﻮﻠﺤﻣ زﺎﺳ

ي نﻮﺘﺳ ﻪﺑ هﺪﺷ MP RP-18

هﺎﮕﺘﺳد رﺰﺗ HPLC

ﯾ و ﺪﺷ ﻖ وﺎﺣ كﺮﺘﺤﻣ زﺎﻓ ﺎﺑ ي

ﺮﻓﺎﺑ و A B

) ﺖﺒﺴﻧ ﻪﺑ 80:20v/v

( ﺮﺟ ﺖﻋﺮﺳ ﺎﺑ ﯾ

نﺎ mL min^-1 8/

0 رد

40°C ﺪﺷ هداد رﻮﺒﻋ .

راﺪﻘﻣ زﺎﺳرﺎﮑﺷآ زا هدﺎﻔﺘﺳا ﺎﺑ GABA

لﻮﻃ رد ﺶﻔﻨﺑ اﺮﻓ 338nm

ﻌﺗ ﯿﯿ ﺪﺷ ﻦ .

2 - 3 - 5 - ﻟﺎﻌﻓ ﯿ ﺸﺑور ﺖ ﯽ دار ﯾ لﺎﮑ ﺎﻫ ي دازآ DPPH

اﺮﺑ ي هزاﺪﻧا ﮔﯿ ﺮ ي ﻓﺮﻇ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ دار رﺎﻬﻣ شور زا ﯾ

لﺎﮑ

2،2 - د ي ﻨﻓ ﯿ ﻞ - 1 -ﭘ ﯿ ﺮﮑ ﯾ ﻫ ﻞ ﯿ زارﺪ ﯾ ﻞ ) (DPPH ﺪﺷ هدﺎﻔﺘﺳا .

اﺮﺑ ي ا ﯾ لﻮﻠﺤﻣ ،لوا ﻪﻠﺣﺮﻣ رد رﻮﻈﻨﻣ ﻦ لﻮﻧﺎﺘﻣ رد DPPH

) 2mM /0 ( ﻬﺗ ﯿﻪ و رد ﯾ ﮏ ﺮﻄﺑ ي ﺷ ﯿ ﻪﺸ ا ي هﻮﻬﻗ ا ي ﻪﺘﺴﺑ

(4)

ﺪﻨﺑ ي هﺪﺷ رد ﺬﻏﺎﮐ ﻣﻮﻟآ ﯿﻨﯿ

،مﻮ ﺎﺗ ﻧﺎﻣز ﯽ ﻪﮐ هدﺎﻔﺘﺳا رد 4 °C

ﺧذ ﯿ هﺮ ﺪﺷ . ﻪﺑ 1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﻪﻧﻮﻤﻧ هرﺎﺼﻋ زا ﺮﺘ ﺎﻫ

ي ﺗ ﯿ رﺎﻤ هﺪﺷ mL

5 لﻮﻠﺤﻣ ﺪﺷ ﻪﻓﺎﺿا DPPH

. وﺎﺣ ﮏﻧﻼﺑ ﻪﻧﻮﻤﻧ ي

1mL

رد هرﺎﺼﻋ ﺪﻗﺎﻓ لﻮﻧﺎﺘﻣ 5mL

لﻮﻠﺤﻣ ﻬﺗ DPPH

ﯿ ﺪﺷ ﻪ . ﻪﺑ

ﻪﻧﻮﻤﻧ ﺎﻣد رد ﺎﻫ ي قﺎﺗا 25°C رﺎﺗ رد ﯾ ﮑ ﯽ تﺪﻣ ﻪﺑ 30 min

ﺪﺷ هداد ﺖﺣاﺮﺘﺳا .

جﻮﻣ لﻮﻃ رد ﻪﻧﻮﻤﻧ بﺬﺟ 517 nm

ﺎﺑ

ﮔ هزاﺪﻧا ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا زا هدﺎﻔﺘﺳا ﯿﺮ

ي ﺪﺷ 11] .[ ﻟﺎﻌﻓ ﯿ ﺖ

ﻨﮐرﺎﻬﻣ ﮔﺪﻨ ﯽ دار ﯾ لﺎﮑ ز لﻮﻣﺮﻓ زا هدﺎﻔﺘﺳا ﺎﺑDPPH

ﯾ ﻪﺒﺳﺎﺤﻣ ﺮ

ﺪﺷ : (%) =

ﻟﺎﻌﻓ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖ ﯽ

دار ﯾ لﺎﮑ DPPH

100 × )) Asample/Acontrol

( - 1 (

نآ رد ﻪﮐ Asample

ﻓ ﻪﻧﻮﻤﻧ بﺬﺟ ﯿ

ﻢﻠ ﺎﻫ ي ﻟﻮﺗ ﯿ ﺪ ي و Acontrol

ﺖﺳا لﺮﺘﻨﮐ ﻪﻧﻮﻤﻧ بﺬﺟ .

2 - 3 - 6 - هزاﺪﻧا و جاﺮﺨﺘﺳا ﮔﯿ

ﺮ ي ﻞﮐ لﻮﻨﻓ راﺪﻘﻣ

راﺪﻘﻣ اﺪﺘﺑا 5g

/0 ﻪﻧاﻮﺟ مﺪﻨﮔ ﻪﻧاد ردﻮﭘ لﻮﻠﺤﻣ ﺎﺑ هدز

80 %

تﺪﻣ ﻪﺑ و طﻮﻠﺨﻣ بآ رد لﻮﻧﺎﺘﻣ 1h

ﺎﺑ ﺲﭙﺳ و ﺪﺷ هدﺰﻤﻫ

ﻓﺎﺻ ﺬﻏﺎﮐ زا هدﺎﻔﺘﺳا ﯽ

ﻦﻤﺗاو 1 ﺎﺑ نآ ﻢﺠﺣ و ﺪﺷ فﺎﺻ

لﻮﻧﺎﺘﻣ لﻮﻠﺤﻣ زا هدﺎﻔﺘﺳا 80

% ﻪﺑ بآ رد 10 mL

ﺪﺷ هﺪﻧﺎﺳر .

ﺲﭙﺳ 5mL /0 ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ زا ﯽ

رد ﻞﻣﺎﮐ نﺪﺷ ﮏﺸﺧ ﺎﺗ

نوآ ﻞﺧاد 50°C

ﺸﺧ ﺪﺷ ﮏ . هزاﺪﻧا يﺮﯿﮔ ناﺰﯿﻣ لﻮﻨﻓ ﻞﮐ ﺎﺑ

ﻦﯿﻟﻮﻓ شور ﻮﭽﻟﺎﮐﻮﯿﺳ-

مﺎﺠﻧا ﺖﻓﺮﮔ . اﺮﺑ ي ا ﯾ ﻗﺎﺑ رﻮﻈﻨﻣ ﻦ ﯿ

هﺪﻧﺎﻤ

رد ﮏﺸﺧ هرﺎﺼﻋ 5mL

/6 ﺪﺷ ﻞﺣ بآ .

ﺲﭙﺳ ﻪﺑ نآ 5mL /0

ﻦﯿﻟﻮﻓ 10

% ﻪﻓﺎﺿا ﺪﺷ هدﺰﻤﻫ ًﻼﻣﺎﮐ ﻪﻧﻮﻤﻧ و ﺪﺷ .

زﺎﺴﭘ 5 min

راﺪﻘﻣ 1mL تﺎﻨﺑﺮﮐ لﻮﻠﺤﻣ عﺎﺒﺷا ﻢﯾﺪﺳ

) 5/

7 ( % هدوﺰﻓا ﺪﺷ .

زا ﺲﭘ 5h /1 ﻬﮕﻧ يراﺪ رد يﺎﻣد قﺎﺗا ﺎﺑ هﺎﮕﺘﺳد ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا

ناﺰﯿﻣ ﻪﻧﻮﻤﻧ بﺬﺟ رد

لﻮﻃ جﻮﻣ 760nm ﺖﺋاﺮﻗ ﺪﺷ و ﻪﺑ

ترﻮﺻ ﺳا لدﺎﻌﻣ gμ

ﯿ ﻟﺎﮔ ﺪ ﯿ ﺮﺑ ﮏ ﺑ ﻪﻧﻮﻤﻧg ﯿ ﺪﺷ نﺎ 12] . [

2 - 3 - 7 - هزاﺪﻧا ﮔﯿ ﺮ ي ﺋﻮﻧوﻼﻓ راﺪﻘﻣ ﯿ

ﻞﮐ ﺪ

ﺋﻮﻧوﻼﻓ راﺪﻘﻣ ﯿ

ﻞﮐ ﺪ ) (TFC شور وﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا هﺎﮕﺘﺳد ﺎﺑ

ﺮﻠﮐ ﯾ ﻣﻮﻟآ ﺪ ﯿﻨﯿ ﮑﺸﺗ سﺎﺳا ﺮﺑ مﻮ ﯿ

ﺋﻮﻧوﻼﻓ ﺲﮑﻠﭙﻤﮐ ﻞ ﯿ

ﺪ - ﻣﻮﻟآ ﯿﻨﯿ مﻮ

ﻌﺗ ﯿﯿ ﺪﺷ ﻦ . اﺮﺑ ي ا ﯾ رﺎﮐ ﻦ 3mL /0 هرﺎﺼﻋ و ﺪﺷ ﮏﺸﺧ

ﻗﺎﺑ ﯿ رد نآ هﺪﻧﺎﻤ 10mL

ﻟﻮﻧﺎﺘﻣ لﻮﻠﺤﻣ ﯽ

ﺮﻠﮐ ﯾ ﻣﻮﻟآ ﺪ ﯿﻨﯿ مﻮ

) AlCl3

( ) 2 % ﻧزو ﯽ / ﻤﺠﺣ ﯽ ( ﮔراﺮﻗ زا ﺲﭘ ،هﺪﺷ طﻮﻠﺨﻣ ﯿﺮ

ي

تﺪﻣ ﻪﺑ قﺎﺗا يﺎﻣد رد 15 min

هداد ﺶﻨﮐاو طﻮﻠﺨﻣ بﺬﺟ ،

رد 420 nm ﺪﺷ يﺮﯿﮔ هزاﺪﻧا .

ﻮﺘﺤﻣ ﯾ تﺎ ﻼﻓ ﺋﻮﻧو ﯿ ﻞﮐ ﺪ زا

ﻨﺤﻨﻣ ﯽ ﻟﺎﮐ ﯿ ﺳاﺮﺒ ﯿ ﺎﻨﺒﻣ ﺮﺑ نﻮ ي ﻮﮐ لدﺎﻌﻣ gμ ﺋ ﺘﺳﺮ ﯿ ﺮﺑ ﻦ ﻪﻧﻮﻤﻧ g

ﺪﺷ ﻪﺒﺳﺎﺤﻣ 12]

. [

2 - 4 - ﻟﺎﻧآ ﯿ رﺎﻣآ ﺰ ي

ا رد ﯾ ﺎﻫرﻮﺘﮐﺎﻓ ﺮﺛا ﻪﻌﻟﺎﻄﻣ ﻦ ي

نﺎﻣز ) ﺢﻄﺳ ﻪﺳ ( ﺖﻈﻠﻏ ،

ﻢ ) 3 ﺢﻄﺳ ( ﺺﺧﺎﺷ ﺮﺑ ﺎﻫ

ي ﻪﻧاﻮﺟ ﻧز ﯽ ﻓﺮﻇ ، ﯿ ﺖ

ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﮐﺮﺗ و ﯿ ز تﺎﺒ ﯾ ﺖﺴ ﻪﻧاﻮﺟ مﺪﻨﮔ ﻪﻧاد لﺎﻌﻓ هدز

رد

ﻓدﺎﺼﺗ ًﻼﻣﺎﮐ حﺮﻃ ﺐﻟﺎﻗ ﯽ

ﺎﻣزآ شور ﻪﺑ ﯾ

رﻮﺘﮐﺎﻓ تﺎﺸ ﯾ

درﻮﻣ ﻞ

ﺰﺠﺗ ﯾ ﻠﺤﺗ و ﻪ ﯿ ﺖﻓﺮﮔ راﺮﻗ ﻞ .

ﺗ ﻪﻤﻫ ﯿ مﺎﺠﻧا راﺮﮑﺗ ﻪﺳ رد ﺎﻫرﺎﻤ

ﺪﺷ . ﻠﺻا تاﺮﺛا ﯽ ﺎﻫرﻮﺘﮐﺎﻓ ﻞﺑﺎﻘﺘﻣ و ي

شور ﺎﺑ هﺪﺷ لﺎﻤﻋا

ﻟﺎﻧآ ﯿ راو ﺰ ﯾ ﺲﻧﺎ ) ANOVA (

ﺎﻘﻣ و ﯾ ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﺗ ﻦ ﯿ ﺎﺑ ﺎﻫرﺎﻤ

ﻣ ﻞﻗاﺪﺣ شور ﯿ

ﮕﻧﺎ ﯿ لﺎﻤﺘﺣا ﺢﻄﺳ رد تﺎﻌﺑﺮﻣ ﻦ )

05 /0

<

(P

ﺖﻓﺮﮔ مﺎﺠﻧا .

ﻟﺎﻧآ ﯿ ﺎﻫﺰ ي رﺎﻣآ ي مﺮﻧ ﺎﺑ ﺎﻫراﺰﻓا ي ﻪﺨﺴﻧ SAS 1/

9

و مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫرادﻮﻤﻧ راﺰﻓا

Excel ﺪﺷ ﻢﺳر .

3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ

3 - 1 - ﺛﺎﺗ ﯿ ﻪﻧاﻮﺟ نﺎﻣز ﺮ ﻧز

ﯽ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ و

ﺪﺳ ﯾ ﺮﺗ نزو ﺮﺑ ﻢ

ﻞﮑﺷ رد ﻪﮐ رﻮﻄﻧﺎﻤﻫ 1

ﻣ هﺪﻫﺎﺸﻣ ﯽ

ﺖﻈﻠﻏ و نﺎﻣز دﻮﺷ

ﺛﺎﺗ ﻢ ﯿ ﻨﻌﻣ ﺮ ﯽ راد ي ﻪﻧاﻮﺟ مﺪﻨﮔ ﻪﻧاد نزو ﺮﺑ هدز

ﺖﺷاد ) 05 /0

≤ (p اﺰﻓا ﺎﺑ و ﯾ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﺶ ﯾ

نﺎﻣز و ﻢ

ﻪﻧاﻮﺟ ﻧز ﯽ ﻪﻧاﻮﺟ مﺪﻨﮔ ﻪﻧاد نزو اﺰﻓا هدز

ﯾ ﺶ ﯾ ﺖﻓﺎ . رﻮﻃ ﻪﺑ ي ﻪﮐ

زا مﺪﻨﮔ نزو 135 g

ﻪﺑ ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ رد 155 g

ﻪﻧﻮﻤﻧ رد مﺮﮔ

ﺗﯿ هﺪﺷ رﺎﻤ mg kg^-1 150

ﺳر لوا زور رد ﻢ ﯿ

ﺪ .

Fig 1 Time course of germinated weight in wheat seeds soaked at different concentration of sodium

glutamate solutions.

*Different capital letters represent significant differences between different germination times in

specific treatment (P < 0.05).

*Different lower-case letters represent significant differences between different treatments at specific

germination times (P < 0.05).

(5)

ﻨﭽﻤﻫ ﯿ ا ﻦ ﯾ دﺎﻘﻣ ﻦ ﯾ ﺎﻣزآ مﻮﺳ و مود زور رد ﺮ ﯾ

ﻪﻧﻮﻤﻧ رد ﻢﻫ ﺶ

ﺎﻫ ﻪﻧﻮﻤﻧ رد ﻢﻫ و ﺪﻫﺎﺷ ي

ﺗ ﯿ اﺰﻓا رﺎﻤ ﯾ ﺶ ﺖﺳا ﻪﺘﺷاد .

ﺎﺘﻧ ﯾ ﺞ

ا زا هﺪﻣآ ﺖﺳﺪﺑ ﯾ

ﻘﺤﺗ ﻦ ﯿ ﺎﺘﻧ ﺎﺑ ﻖ ﯾ ﻟﺰﻧاﺮﺑ ﺞ ﯿﻨ ﯽ نارﺎﮑﻤﻫ و )

2018 (

ﺎﮐﻮﮔﺎﻧ و ) 2005 ( ﺖﺷاد ﺖﻘﺑﺎﻄﻣ 8]

و 9 . [

ﺎﭽﻧاوﻮﮐ ي نارﺎﮑﻤﻫ و )

2014 ( ندوﺰﻓا ﻪﮐ ﺪﻧدﺮﮐ رﺎﻬﻇا

اﺰﻓا ﺚﻋﺎﺑ ﻢ ﯾ

راﺪﻘﻣ ﺶ ﻢﻫ و GABA

ﻨﭼ ﯿ ﻦ

ﺮﺳ ﺰﺘﻨﺳ ﯾﻊ ﺌﺗوﺮﭘ ﺮﺗ ﯿ ﻦ ﺎﻫ ي ﻧ درﻮﻣ ﯿ مﺎﮕﻨﻫ رد ﻪﻧاد زﺎ ﻪﻧاﻮﺟ

ﻧز ﯽ

ﻣ و هﺪﺷ ﯽ ﭘ ﺰﺘﻨﺳ ﺎﺑ ﺪﻧاﻮﺗ ﯿ

ﺶ ﺎﻫزﺎﺳ ي ﺳا ﻪﺧﺮﭼ ﯿ ﺳ ﺪ ﯿ ﺮﺘ ﯾ ﻪﺑ ﮏ

ﺮﺳ ﺪﺷر ﯾ ﻊ ﺪﻨﮐ ﮏﻤﮐ ﻪﻧاد ﺮﺗ 13]

. [

3 - 1 - 1 - ﺛﺎﺗ ﯿ ﻪﻧاﻮﺟ نﺎﻣز ﺮ ﻧز

ﯽ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ و ﯾ

ﻢ

ﻪﻧاد داﺪﻌﺗ ﺮﺑ

ﻞﮑﺷ رد ﻪﮐرﻮﻄﻧﺎﻤﻫ 2

ﻣ هﺪﻫﺎﺸﻣ ﯽ ﻪﻧاﻮﺟ نﺎﻣز ﺮﺛا ددﺮﮔ ﻧز

ﯽ و

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﯾ

ﻪﻧاﻮﺟ ﻪﻧاد داﺪﻌﺗ ﺮﺑ ﻢ ﻌﻤﻫدز

ﻨ ﯽ راد ي دﻮﺑ

) P<0.05 .(

رﻮﻃ ﻪﺑ ﯾ زا ﻪﻧاﻮﺟ داﺪﻌﺗ ﻪﮑ 50

% ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ رد

ﻪﺑ لوا زور رد 60

% ﺳر مﻮﺳ زور رد ﯿ

ﺷﺎﻧ ﻪﮐ ﺪ ﯽ ﺛﺎﺗ زا ﯿ ﺮ

ﻨﻌﻣ ﯽ راد ي ﻣ نﺎﻣز ﯽ ﺪﺷﺎﺑ . ﭽﻤﻫ ﯿ ﺎﺘﻧ ﻦﻨ ﯾ ﻣ نﺎﺸﻧ ﺞ ﯽ ﺗ ﻪﮐ ﺪﻫد ﯿ رﺎﻤ

ﺖﻈﻠﻏ ﺎﺑ ﺎﻫ ي mg kg^-1 50

و 100 ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﯾ

ﺛﺎﺗ ﻢ ﯿ ﺮ

ﻨﻌﻣ ﯽ راد ي ﻪﻧاﻮﺟ ﻪﻧاد داﺪﻌﺗ ﺮﺑ ﺎﻘﻣ رد هدز

ﯾ ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ﺎﺑ ﻪﺴ

ﻟو ﻪﺘﺷاﺪﻧ ﯽ اﺰﻓا ﯾ اﺰﻓا ﺚﻋﺎﺑ ﺖﻈﻠﻏ ﺶ ﯾ

ﺮﺤﺗ ﺶ ﯾ ﻪﻧاﻮﺟ ﮏ ﻧز ﯽ و

اﺰﻓا ﯾ ﻪﻧاﻮﺟ ﻪﻧاد داﺪﻌﺗ ﺶ ﺪﺷ هدز

.

Fig 2 Time course of germinated seed numbersin wheat seeds soaked at different concentration of

sodium glutamate solutions.

رﻮﻄﺑ ي ﺑ ﻪﮐ ﯿ ﺮﺘﺸ ﯾ ﻪﻧاﻮﺟ ﺪﺻرد ﻦ ﻧز

ﯽ ) 99 (%

ﻪﻧﻮﻤﻧ ﻪﺑ طﻮﺑﺮﻣ

ﺗﯿ ﺎﺑ هﺪﺷ رﺎﻤ mg kg^-1

150 ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﯾ

مﻮﺳ زور رد ﻢ

ﻪﻧاﻮﺟ ﻧز ﯽ دﻮﺑ . اﯾ ﻣﺎﺗﻮﻨ ﯽ نارﺎﮑﻤﻫ و )

1971 ( ﻪﮐ ﺪﻨﺘﺷاد رﺎﻬﻇا اﺰﻓا

ﯾ ﻣﺎﺗﻮﻠﮔ راﺪﻘﻣ ﺶ ﯿ

ﺳا ﮏ ﯿ ﺧ بآ رد ﺪ ﯿ

نﺪﻧﺎﺴ ﺚﻋﺎﺑ ﻮﺟ ﻪﻧاد

اﺰﻓا ﯾ ﻧز ﻪﻧاﻮﺟ ﺖﻋﺮﺳ ﺶ ﯽ

ﻣ ﯽ دﻮﺷ 14] .[

ﯽ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ و

ﺪﺳ ﯾ ﻪﻧاﻮﺟ لﻮﻃ ﺮﺒﻤ

ﻞﮑﺷ رد ﻪﮐ رﻮﻄﻧﺎﻤﻫ 3

ﻣ هﺪﻫﺎﺸﻣ ﯽ ﻪﻧاﻮﺟ نﺎﻣز ﺮﺛا ددﺮﮔ ﻧز

ﯽ و

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﯾ

ﻨﻌﻣ ﻪﻧاﻮﺟ لﻮﻃ ﺮﺑ ﻢ ﯽ

دﻮﺑ راد ) 05 /0 .(p≤

ﻨﻌﻣ فﻼﺘﺧا لوا زور رد ﯽ

ﺑ راد ﯿ ﺎﺷ ﻪﻧﻮﻤﻧ ﻦ ﺎﺳ و ﺪﻫ

ﯾ ﻪﻧﻮﻤﻧ ﺮ ﺎﻫ

ﺖﺷاﺪﻧ دﻮﺟو ﻪﻧاﻮﺟ لﻮﻃ ﺮﻈﻧ زا )

05 /0

>

(p ﻟو ، ﯽ مﻮﺳ زور رد

اﯾ اﺰﻓا ﺎﺑ و هدﻮﺑ دﻮﻬﺸﻣ فﻼﺘﺧا ﻦ ﯾ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﺶ ﯾ

ﻢ

ﻧ ﻪﻧاﻮﺟ لﻮﻃ ﯿ

ﻨﻌﻣ رﻮﻄﺑ ﺰ ﯽ

اﺰﻓا راد ﯾ ﺶ ﯾ ﺖﻓﺎ . ﺑﯿ ﺮﺘﺸ ﯾ لﻮﻃ ﻦ

ﻪﻧاﻮﺟ ) 11mm ( ﻪﻧﻮﻤﻧ ﻪﺑ طﻮﺑﺮﻣ ﺗ ﻪﺑ

ﯿ رﺎﻤ mg kg^-1 150

دﻮﺑ مﻮﺳ زور و ﻢ ﺘﻧﯿ.

ا زا هﺪﻣآ ﺖﺳﺪﺑ ﻪﺠ ﯾ

ﻦ

ﺎﺘﻧ ﺎﺑ ﻖﺑﺎﻄﻣ ﺶﻫوﮋﭘ ﯾ

نارﺎﮑﻤﻫ و ﻮﻧر ﺞ )

2010 ( دﻮﺑ 15] . [

Fig 3 Time course of germ length in wheat seeds soaked at different concentration of sodium

glutamate solutions.

ﻢ

ﻮﺘﺤﻣ ﺮﺑ ي ﺋﻮﻧوﻼﻓ ﯿ ﻞﮐ ﺪ

نﺎﻤﻫ ﻞﮑﺷ رد ﻪﮐ رﻮﻃ 4

هﺪﻫﺎﺸﻣ ﻣ ﯽ ﺛﺎﺗ دﻮﺷ ﯿ ﻧز ﻪﻧاﻮﺟ نﺎﻣز ﺮ ﯽ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ راﺪﻘﻣ و ﯾ

ﻮﺘﺤﻣ ﺮﺑ ﻢ ي

ﺋﻮﻧوﻼﻓ ﯿ ﻪﻧﻮﻤﻧ ﻞﮐ ﺪ ﺎﻫ

ﻨﻌﻣ ﯽ دﻮﺑ راد ) 05 /0 .(ﺑp≤

ﯿ ﺮﺘﺸ ﯾ ﺋﻮﻧوﻼﻓ راﺪﻘﻣ ﻦ ﯿ

زور رد ﻞﮐ ﺪ

ﻪﻧاﻮﺟ لوا زﻧ ﯽ رﻮﻄﺑ نآ راﺪﻘﻣ نﺎﻣز ﺖﺷﺬﮔ ﺎﺑ و ﺪﺷ ﺪﻫﺎﺸﻣ

ﻨﻌﻣ ﯽ ﺶﻫﺎﮐ راد ﯾ

ﺖﻓﺎ . ﻨﭽﻤﻫ ﯿ نﺎﻣز ﻪﻤﻫ رد ﻦ ﺑ ﺎﻫ

ﯿ ﺮﺘﺸ ﯾ راﺪﻘﻣ ﻦ

ﺋﻮﻧوﻼﻓ ﯿ ﺗ ﻪﺑ طﻮﺑﺮﻣ ﻞﮐ ﺪ ﯿ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺎﺑ رﺎﻤ ﯾ

ﻢ mg kg^-

1001

دﻮﺑ . ﺋﻮﻧوﻼﻓ ﯿ ﻪﻧاﻮﺟ تﺪﻣ لﻮﻃ رد ﻞﮐ ﺪ ﻧز

ﯽ ﺸﻫﺎﮐ ﺪﻧور ﯽ

زا و ﻪﺘﺷاد μg CE g^-1 47

ﻪﺑ 38 زا و ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ رد μg

(6)

CE g^-1 46 ﻪﺑ 30 ﺗ ﻪﻧﻮﻤﻧ رد ﯿ

ﺎﺑ رﺎﻤ mg kg^-1 150

تﺎﻣﺎﺗﻮﻠﮔ

ﺪﺳ ﯾ ﺳر ﻢ ﯿ ﺪ.

ﺋﻮﻧوﻼﻓ راﺪﻘﻣ رد ﺶﻫﺎﮐ ﯿ

ﻣ ار ﻞﮐ ﺪ ﯽ رﺎﻬﻣ ﻪﺑ ناﻮﺗ

دار ﯾ لﺎﮑ ﺎﻫ ي ﻟﻮﺗ دازآ ﯿ ﻟﻮﺑﺎﺘﻣ ﺮﺛا رد هﺪﺷ ﺪ ﯿ

ﻣ ﻢﺴ ﯿ رﺪﻨﮐﻮﺘ ي ﺑ ﯿ نﺎ

دﺮﮐ . ﺘﻧﯿ ا زا هﺪﻣآ ﺖﺳﺪﺑ ﻪﺠ ﯾ

ﺎﺘﻧ ﺎﺑ ﺶﻫوﮋﭘ ﻦ ﯾ

نارﺎﮑﻤﻫ و ﻮﻧر ﭻ

) 2010 ( ﻟﺰﻧاﺮﺑ و ﯿﻠﻨ ﯽ نارﺎﮑﻤﻫ و )

2018 ( ﺖﺷاد ﺖﻘﺑﺎﻄﻣ

8] و 9 . [

Fig 4 Time course of total flavonoid contentin wheat seeds soaked at different concentrations of

sodium glutamate.

ﻢ

ﻞﮐ لﻮﻨﻓ ﺮﺑ

ﻞﮑﺷ 5 ﻣ نﺎﺸﻧ ﯽ ﻪﮐ ﺪﻫد ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﻂﻘﻓ ﯾ

ﻣ ﺮﺑ ﻢ ﯿ ناﺰ

ﺛﺎﺗ ﻞﮐ لﻮﻨﻓ ﯿ

ﻨﻌﻣ ﺮ ﯽ راد ي ﺖﺷاد ) 05 /0 .(p≤

رﻮﻃ ﻪﺑ ي اﺰﻓا ﺎﺑ ﻞﮐ لﻮﻨﻓ ﻪﮐ ﯾ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ راﺪﻘﻣ ﺶ ﯾ

ﻪﻓﺎﺿا ﻢ

هﺪﺷ اﺰﻓا ﺪﻧور ﯾ ﺸ ﯽ زا و ﻪﺘﺷاد μg GAEg^-1

2432 ﻪﻧﻮﻤﻧ رد

ﺪﻫﺎﺷ ﻪﺑ μg GAEg^-1 4589

ﺗ ﻪﻧﻮﻤﻧ رد ﯿ

ﺎﺑ هﺪﺷرﺎﻤ mg kg^-1

ﺳر ﻢ ﯿ ﺪ.

اﺰﻓا ﯾ ﮐﺮﺗ راﺪﻘﻣ ﺶ ﯿ

ﻟﻮﻨﻓ تﺎﺒ ﯽ رد

ﻃ ﯽ ﻪﻧاﻮﺟ ﻣ ار مﺪﻨﮔ ﻪﻧاد ندز ﯽ

اﺰﻓا ﻪﺑ ناﻮﺗ ﯾ

ﺑ ﺶ ﯿ ﺰﺠﺗ و ﺰﺘﻨﺳﻮ ﯾﻪ

ﻟﯿ ﻨﮕ ﯿ ﺎﺳ و ﻦ ﯾ ﻠﭘ ﺮ ﯿ ﺎﻫﺮﻤ ي د ﯾ ﻟﻮﻠﺳ هراﻮ ﯽ داد ﺖﺒﺴﻧ 16]

. [

ﻢﻫ ﻨﭼ ﯿ آﺮﻓ ﻦ ﯾ ﻪﻧاﻮﺟ ﺪﻨ ﻧز ﯽ ﮑﻤﻣ ﺑ ﺖﺳا ﻦ ﯿ ﮐﺮﺗ ﺰﺘﻨﺳﻮ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ

اﺰﻓا ار ﯾ ا ﻪﮐ ﺪﻫد ﺶ ﯾ

اﺰﻓا ﻦ ﯾ ﺮﻃ زا ﺶ ﯾ ﺰﻧآ نﺪﺷ لﺎﻌﻓ ﻖ ﯾﻢ

ﺎﻫ ي

ﻨﻓﯿ ﻞ ﻧﻻآ ﯿ ﻣآ ﻦ ﯿ ﻟﻮﻨ ﯿ ﺗﺎﺗﻮﻠﮔ و زﺎ ﯿ

طﻮﺑﺮﻣ زاﺮﻔﺴﻧاﺮﺗ سا نﻮ

ﺖﺳا 17] . [

Fig 5 Time course of total phenol contentin wheat

seeds soaked at different concentrations of sodium glutamate.

ﯽ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ و

ﺪﺳ ﯾ راﺪﻘﻣ ﺮﺑ ﻢ GABA

ﻞﮑﺷ 6 ﻣ نﺎﺸﻧ ﯽ ﺛﺎﺗ ﻂﻘﻓ ﻪﮐ ﺪﻫد ﯿ

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ راﺪﻘﻣ ﺮ ﯾ

ﺮﺑ ﻢ

راﺪﻘﻣ ﻨﻌﻣGABA ﯽ هدﻮﺑ راد ) 05 /0 (p≤

ﻪﻧاﻮﺟ نﺎﻣز ﺮﺛا و ﻧز

ﯽ

ﻏﯿ ﻨﻌﻣﺮ ﯽ دﻮﺑ راد .

Fig 6 Time course of GABA contentin wheat seeds soaked at different concentrations of sodium

glutamate.

*Different Capital letters represent significant differences between different germination times in

(7)

رﻮﻃ ﻪﺑ ي راﺪﻘﻣ ﻪﮐ زا لوا زور ردGABA

mg 100g^-1 169

ﻪﺑ 302 ﺗ ﻪﻧﻮﻤﻧ رد ﯿ

ﺎﺑ هﺪﺷرﺎﻤ mg kg^-1

ﻢ

ﺳر ﯿ ﺪ . اﺰﻓا ﯾ ﻣ ﺶ ﯿ ناﺰ ﻣآ ﺎﻣﺎﮔ ﯿ ﺗﻮﺑ ﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪ اﺰﻓا ﺎﺑ ﯾ ﺖﻈﻠﻏ ﺶ

ﺧ بآ رد ﻢ ﯿ

ﺴﮐﻮﺑﺮﮐد ﻪﺑ ار نﺪﻧﺎﺴ ﯿ

ﺳﻼ ﯿ ﺳا نﻮ ﯿ ﺪ

ﻣﺎﺗﻮﻠﮔ ﯿ ﺰﻧآ ﻂﺳﻮﺗ ﮏ ﯾ

ﺎﻣﺎﺗﻮﻠﮔ ﻢ ﺴﮐﻮﺑﺮﮐد ت ﯿ

ﻃ رد زﻼ ﯽ ﻪﻧاﻮﺟ -

ﻧز ﯽ داد ﺖﺒﺴﻧ .

ﻟوا ﻞﺣاﺮﻣ زا ﺲﭘ ﯿ

ﻪﻧاﻮﺟ ﻪ ﻧز ﯽ ﻣآ ﺎﻣﺎﮔ ﯿ ﻮﻨ

ﺗﻮﺑ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪ ﻂﺳﻮﺗ ﯾ ﻣ ﻞﺧاد ﻪﺑ ﻞﻣﺎﺣ ﮏ ﯿ

رﺪﻨﮐﻮﺘ ي ﻞﻘﺘﻨﻣ

ﺴﮐﻮﺳ ﻪﺑ و هﺪﺷ ﯿﻨﯿ

ﻤﺳ ﮏ ﯽ ﺋﺪﻟآ ﯿ ﺪﺒﺗ ﺪ ﯾ ﺪﺒﺗ زا ﺲﭘ و ﻞ ﯾ

ﻪﺑ نآ ﻞ

ﺴﮐﻮﺳ ﯿ ﺳا ﻪﺧﺮﭼ دراو تﺎﻨ ﯿ

ﺳ ﺪ ﯿ ﺮﺘ ﯾ ﻣ ﮏ ﯽ ﺎﻬﻧ رد و دﻮﺷ ﯾ

ﺖ

ﻣ ﯽ ﻮﻠﮔ ﻪﺑ ًادﺪﺠﻣ ﺪﻧاﻮﺗ ﻣﺎﺗ

ﯿ ﺳا ﮏ ﯿ ﺪﺒﺗ ﺪ ﯾ راﺪﻘﻣ اﺬﻟو دﻮﺷ ﻞ ﺎﻣﺎﮔ

ﻣآ ﯿ ﺗﻮﺑ ﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪ ﻃ رد ﯽ ﻪﻧاﻮﺟ نﺎﻣز ﻧز

ﯽ ﻗﺎﺑ ﺖﺑﺎﺛ ﯽ

ﺪﻧﺎﻣ 18] . [

3 - 1 - 6 - ﺛﺎﺗ ﯿ ﻧز ﻪﻧاﻮﺟ نﺎﻣز ﺮ ﯽ

تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ و

ﺪﺳ ﯾ ﻓﺮﻇ ﺮﺑ ﻢ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ

ﺎﺘﻧ ﯾ ﻟﺎﻧآ ﺞ ﯿ راو ﺰ ﯾ ﺎﻘﻣ و ﺲﻧﺎ ﯾ

ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﻣ نﺎﺸﻧ ﻦ ﯽ ﺮﺛا ﻪﮐ ﺪﻫد

ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ راﺪﻘﻣ ﯾ

ﺮﻇ ﺮﺑ ﻢ ﻓﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻨﻌﻣ ﯽ راد

دﻮﺑ ) 05 /0

≤ (p ﻪﻧاﻮﺟ نﺎﻣز و ﻧز

ﯽ ﺛﺎﺗ ﯿ ﻨﻌﻣ ﺮ ﯽ راد ي ﺖﺷاﺪﻧ نآ ﺮﺑ

) 05 /0

˃ .(p

Fig 7 Time course of DPPH scavenging wheat seeds soaked at different concentrations of sodium

glutamate

*Different Capital letters represent significant differences between different germination times in

اﺰﻓا ﯾ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﺶ ﯾ

اﺰﻓا ﺚﻋﺎﺑ ﻢ ﯾ

ﺶ ﻟﺎﻌﻓ ﯿ ﺸﺑور ﺖ ﯽ

دار ﯾ لﺎﮑ ﺎﻫ ي دازآ هﺪﺷ DPPH

ﺖﺳا . رﻮﻃ ﻪﺑ ي ﻢﮐ ﻪﮐ ﺮﺗ ﯾ ﻦ

ﻟﺎﻌﻓ ﯿ ﺸﺑور ﺖ ﯽ دار ﯾ لﺎﮑ دازآ ﺎﻫ ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ﻪﺑ طﻮﺑﺮﻣ DPPH

) 29 (%

ﺑ و ﯿ ﺮﺘﺸ ﯾ ﺗ ﻪﻧﻮﻤﻧ ﻪﺑ طﻮﺑﺮﻣ راﺪﻘﻣ ﻦ ﯿ

ﺎﺑ هﺪﺷرﺎﻤ mg

kg^-1 150 ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﯾ

ﻢ ) 57 (%

دﻮﺑ . ﻓا اﺰ ﯾ ﻓﺮﻇ ﺶ ﯿ ﺖ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻟد ﻪﺑ ﯿ اﺰﻓا ﻞ ﯾ ﻣ ﺶ ﯿ ﮐﺮﺗ ناﺰ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻪﻧاد رد

ﻪﻧاﻮﺟ دازآ و ﻪﻧاد بآ بﺬﺟ ﺮﺛا رد ﻪﻧاد ﺰﻐﻣ نﺪﺷ مﺮﻧ و هدز

ﮐﺮﺗ نﺪﺷ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ داد ﺖﺒﺴﻧ ﻪﻧاد رد رﻮﺼﺤﻣ 19]

و 20 .[

ا ﺮﺑ هوﻼﻋ ﯾ

ﺪﺒﺗ زا ﺲﭘ ﻦ ﯾ

ﻣﺎﺗﻮﻠﮔ ﻞ ﯿ ﺳا ﮏ ﯿ ﻪﺑ ﺪ ﻣآ ﺎﻣﺎﮔ ﯿ ﻮﻨ

ﺗﻮﺑ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪ ﻞﺧاد رد ،لﻮﻠﺳ ﻣآ ﺎﻣﺎﮔ ﯿ ﺗﻮﺑ ﻮﻨ ﯿﺮ ﯾ ﺳا ﮏ ﯿ ﺪ ﻪﺑ

ﻟﻮﺑﺎﺘﻣ ﯿ ﺖ ﺎﻫ ي د ﻂﺳاو ﯾ ﺮﮕ ي ﺪﺒﺗ ﯾ ﺳا ﻪﺧﺮﭼ دراوو ﻞ ﯿ

ﺳ ﺪ ﯿ ﺮﺘ ﯾ ﮏ

ﻣ ﯽ ﺎﻬﻧ رد ﻪﮐ دﻮﺷ ﯾ

ﻣ ﺖ ﯽ اﺰﻓا ﺚﻋﺎﺑ ﺪﻧاﻮﺗ ﯾ

ﮐﺮﺗ ﺰﺘﻨﺳ ﺶ ﯿ

تﺎﺒ

ﻟﻮﻨﻓ ﯽ ﻓﺮﻇ و دﻮﺷ ﯿ

ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ اﺰﻓا ار ﯾ ﺪﻫد ﺶ 18] .[

4 - ﺘﻧ ﯿ ﻪﺠ ﮔﯿ ﺮ ي

ﻪﻧاﻮﺟ ﻧز ﯽ ﻪﻧاد ﺎﻫ ي ﮐﺮﺗ ﺰﺘﻨﺳ ﺚﻋﺎﺑ تﻼﻏ ﯿ

ﺗ شزراﺎﺑ تﺎﺒ ﺬﻐ

ﯾﻪ ا ي

ز و ﯾ ﻣ لﺎﻌﻓ ﺖﺴ ﯽ

ادﻮﺷ ﯾ ﻘﺤﺗ ﻦ ﯿ ﺳرﺮﺑ فﺪﻫ ﺎﺑ ﻖ ﯽ

ﺛﺎﺗ ﯿ نﺎﻣز ﺮ

ﻪﻧاﻮﺟ ﻧز ﯽ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ راﺪﻘﻣ و ﯾ

ﻣ ﺮﺑ ﻢ ﯿ ﮐﺮﺗ ناﺰ ﯿ ز تﺎﺒ ﯾ ﺖﺴ

ﻣآﺎﻣﺎﮔ راﺪﻘﻣ و لﺎﻌﻓ ﯿ

ﺮﺗﻮﺑﻮﻨ ﯾ ﺖﻓﺮﮔ مﺎﺠﻧا ﮏ .

ﺎﻣﺎﮔ راﺪﻘﻣ

ﻣآ ﯿ ﺮﺗﻮﺑﻮﻨ ﯾ ﺳا ﮏ ﯿ ﻪﻧاﻮﺟ مﺪﻨﮔ ﻪﻧاد رد ﺪ راﺪﻘﻣ ﻪﺑ ﻪﺘﺴﺑاو هدز

ﻧﻻﻮﻃ و ﺖﺳا ﻢ ﯽ

نﺪﺷ ﺮﺗ ﻪﻧاﻮﺟ نﺎﻣز ﻧز

ﯽ ﺑ ﯿ ﺶ

زا 24h ﺛﺎﺗ ﯿﺮ ي دراﺪﻧ نآ راﺪﻘﻣ ﺮﺑ .

اﺮﺑ هوﻼﻋ ﯾ ﺎﺘﻧ ﻦ ﯾ داد نﺎﺸﻧ ﺞ

اﺰﻓا ﻪﮐ ﯾ ﺪﺳ تﺎﻣﺎﺗﻮﻠﮔ ﺖﻈﻠﻏ ﺶ ﯾ

ﺤﻣ رد ﻢ ﯿ ﻪﻧاﻮﺟ ﻂ ﻧز ﯽ ﺚﻋﺎﺑ

اﺰﻓا ﯾ ﮐﺮﺗ راﺪﻘﻣ ﺶ ﯿ

ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻣ ﻪﮐ ﺪﺷ ﯽ ﻟﺪﺑ ﺪﻧاﻮﺗ ﯿ نﺪﺷ لﺎﻌﻓ ﻞ

ﺴﻣ ﯿ ﺎﻫﺮ ي ﻟﻮﺑﺎﺘﻣ ﯿ ﮑ ﯽ ﻟﻮﺑﺎﺘﻣ ﺰﺘﻨﺳ و ﯿ

ﺖ ﺎﻫ ي ﭘ ﯿ ﺶ ا زﺎﺳ ﯾ ﮐﺮﺗ ﻦ ﯿ تﺎﺒ

ﺪﺷﺎﺑ . ﺶﻫﺎﮐ ﮐﺮﺗ راﺪﻘﻣ رد هﺪﺷ هﺪﻫﺎﺸﻣ ﯿ

ﺋﻮﻧوﻼﻓ تﺎﺒ ﯿ

ﺪ ي رد

ﻃ ﯽ ﻪﻧاﻮﺟ نﺎﻣز ﻧز

ﯽ ﺜﻨﺧ ﺐﺒﺳ ﻪﺑ ﯽ

دار ندﺮﮐ ﯾ لﺎﮑ ﺎﻫ ي دازآ

ﻟﻮﺗ ﯿ ﻃ رد هﺪﺷ ﺪ ﯽ

ﻪﻧاﻮﺟ ﻧز ﯽ ﺖﺳا . ﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯾ

ﺖﺳﺪﺑ ﺞ

ا رد هﺪﻣآ ﯾ ﻘﺤﺗ ﻦ ﯿ ﺮﺘﺒﺳﺎﻨﻣ ﻖ ﯾ ﻟﻮﺗ ظﺎﺤﻟ زا نﺎﻣز ﻦ ﯿ

ﻣآ ﺎﻣﺎﮔ ﺪ ﯿ ﻮﻨ

ﺮﺗﻮﺑ ﯾ ﺳا ﮏ ﯿ ﮐﺮﺗ و ﺪ ﯿ اراد تﺎﺒ ي ﻟﺎﻌﻓ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ د مﺪﻨﮔ ر

ﻪﻧاﻮﺟ نﺎﻣز هدز 24 h

ﺖﻈﻠﻏ و mg kg^-1

150 تﺎﻣﺎﺗﻮﻠﮔ

ﺪﺳ ﯾ ﻣ و ﺖﺳا ﻢ ﯽ

ا زا هدﺎﻔﺘﺳا ﺎﺑ ناﻮﺗ ﯾ

ﻟﻮﺗ ﺖﻬﺟ شور ﻦ ﯿ

ﻪﻧاد ﺪ

ﻪﻧاﻮﺟ مﺪﻨﮔ ﺬﻐﺗ شزرا ،هدز

ﯾﻪ ا ي نآ زا ﻞﺻﺎﺣ درآ و مﺪﻨﮔ ﻪﻧاد

اﺰﻓا ار ﯾ ﻪﺑ و ﺶ ﺬﻐﺗ ﻞﻤﮑﻣ ناﻮﻨﻋ ﯾﻪ

ا ي ﻟﻮﺗ رد ﺶﺨﺑ ﺖﻣﻼﺳ و ﯿ

ﺪ

هدروآﺮﻓ ﺎﻫ ي درآ ي ﮐ ،نﺎﻧ ﺪﻨﻧﺎﻣ ﯿ

ﮏ و ...

رﺎﺸﻓ ﺶﻫﺎﮐ ﺖﻬﺟ

د لﺮﺘﻨﮐ و نﻮﺧ ﯾ

دﺮﮐ هدﺎﻔﺘﺳا نآ زا ﺖﺑﺎ .

5 - ﻊﺑﺎﻨﻣ

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Effect of sodium glutamate pretreatment on physicochemical properties and bioactive compounds in germinated wheat

PashaeiBahram R.¹, Javadi A.^2*, Bodbodak S.³, Arajkhodaei M.⁴

1. Ph.D. Student of Department of Food Science and Technology, Mamagan Branch, Islamic Azad University, Mamagan, Iran.

2. Professor ofDepartment of Food Hygiene, Tabriz Medical Science, Islamic Azad University, Tabriz, Iran.

3. Assistant Professor of Department of Food Science and Technology, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.

4. Assistant Professor of Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT ARTICLE INFO

Article History:

Received 2022/ 04/ 16 Accepted 2022/ 05/ 25

Keywords:

Sodium glutamate, Gama-Aminobutyric acid, Germinated wheat, Germination.

The aim of the study was to investigate the effect of sodium glutamate pretreatment, as a substrate, on the production of gamma aminobutyric acid during wheat germination. For this purpose, wheat grains (Alvandvariety) were treated with different amounts of sodium glutamate (0, 50, 100, and 150 mg kg^-1) during 72 h germination.

Physical properties (such as germinated seed weight, number of germinated seeds and germination length) and bioactive compounds contents (such as gamma aminobutyric acid, total flavonoids, total phenols and free radical scavenging activity of DPPH) were measured at 24, 48 and 72 h time intervals. The results showed that germinated seed weight and number of germinated seeds increased with increase of sodium glutamate concentration and germination time. Also, the total flavonoids contents decreased in all samples during germination time and the increase of glutamic acid did not have a significant effect on its amount. The highest levels of total phenol, gamma aminobutyric acid and antioxidant capacity were observed in the sample treated with150 mg kg^-1 on the first day of germination equal to 4589 μg GAE g^-1, 303 mg 100 g-1 and 57%, respectively. In all treatments, the amount of these compounds did not change significantly during germination time (p

>0.05). Finally, it was concluded that the 24 hours germination time and the 150 mg kg^-1 glutamate was the most appropriate treatment to increase the biosynthesis of gamma-aminobutyric acid and anatioxidant activity in germinated wheat grains.

DOI: 10.22034/FSCT.19.128.83

DOR: 20.1001.1.20088787.1401.19.128.17.2

*Corresponding Author E-Mail:

[email protected]

Journal of Food Science and Technology (Iran)

Homepage:www.fsct.modares.ir Scientific Research