ﺮان ﯾا ﯽﯾ ﻏﺬا ﻊﯾ ﺻﻨﺎ و ﻋﻠﻮم ﻣﺠﻠﻪ

(1)

ﺛﺄﺗ ﯿ و نزا ﺮ ﺎﻏذ

ل لﺎﻌﻓ ﺮﺑ ﻟﻮﺗﺎﭘ ﯿ ﻦ هﺮﺘﻧﺎﺴﻨﮐرد ﺳ

ﯿ ﺐ

ﺸﺑ نﺎﺸﻟا ﯿﺮ

1ي ﺘﻣ ارﺎﺳ ، ﯿﻨ

2ﯽ

، *

ﻣرآ ماﺮﻬﺷ ﯿ

3هﺪ

1 - ﻮﺠﺸﻧاد ي ﺳﺎﻨﺷرﺎﮐ ﯽ ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،ﺪﺷرا ﯽ

ﻮﺧ ، ي ا ، ﯾ .ناﺮ

2 - دﺎﺘﺳا ﯾ ﺎﻨﺻ مﻮﻠﻋ هوﺮﮔ رﺎ ﯾ

اﺬﻏ ﻊ ﯾ ﯽ ﻮﺧ ﺪﺣاو،

ي ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ، ﯽ

ﻮﺧ ، ي ا ، ﯾ ناﺮ .

3 - ﺸﻧاد ﯿ زروﺎﺸﮐ هﺪﮑﺸﻧاد رﺎ ي

ﻣورا هﺎﮕﺸﻧاد ، ﯿ

ا ،ﻪ ﯾ ناﺮ .

تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ

ﮑﭼ

ﯿ هﺪ

رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :

رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 09 / 09 / 1400

رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 03 / 11 / 1400

تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :

،ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ

ﻟﻮﺗﺎﭘ ﯿ

،ﻦ

،نزُا

،لﺎﻌﻓ لﺎﻏز

ﺳ هﺮﺘﻧﺎﺴﻨﮐ ﯿ

.ﺐ

DOI: 10.52547/fsct.19.123.189

DOR: 20.1001.1.20088787.1401.19.123.21.6

لﻮﺌﺴﻣ

تﺎﺒﺗﺎﮑﻣ : com . gmail

@ sara . Matinii

ﻟﻮﺗﺎﭘ ﯿ ﻦ ﯾ ﻠﺻا ﻞﮑﺸﻣ ﮏ ﯽ

اﺮﺑ ي ﻣ بآ ﺖﻌﻨﺻ رد صﻮﺼﺧ ﻪﺑ نﺎﺴﻧا ﺖﻣﻼﺳ ﯿ

هﺮﺘﻧﺎﺴﻨﮐ و هﻮ

ﻣ ﯽ ﺪﺷﺎﺑ ﻟﻮﺗﺎﭘ ﯽﻧﺎﻬﺟ دراﺪﻧﺎﺘﺳا. ﯿ

هﻮﯿﻣ بآ رد ﻦ دوﺪﺣ رد ﺎﻫ

50 ppb ﯽﻣ ﺪﺷﺎﺑ ﺳرﺮﺑ . ﯽ نﺎﺸﻧ ﺎﻫ

ﻣ ﯽ شور زا هدﺎﻔﺘﺳا ﻪﮐ ﺪﻫد ﺎﻫ

ي ﻓ ﻒﻠﺘﺨﻣ ﯿﺰ

ﯾ ﮑ ﯽ ﺷ ، ﯿﻤ ﯿﺎ ﯾ ﯽ ﺑ و ﯿ ژﻮﻟﻮ ﯾ ﮑ ﯽ ﻣ ﯽ راﺪﻘﻣ ﺪﻧاﻮﺗ

ﻟﻮﺗﺎﭘ ﯿ ﺪﻫد ﺶﻫﺎﮐ ار ﻦ ﻤﻫ رد.

ﯿ ﻣ ،ﺎﺘﺳار ﻦ ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﻪﻧﻮﻤﻧ رد ﻦ ﺎﻫ

ي ﺳ هﺮﺘﻧﺎﺴﻨﮐ ﯿ

ﺳو ﻪﺑ ﺐ ﯿ ﻪﻠ

نآ ماﻮﺗ ﺮﺛا و لﺎﻌﻓ لﺎﻏز ،نزُا زرا درﻮﻣ ﺎﻫ

ﯾﺑﺎ ﯽ ﺖﻓﺮﮔ راﺮﻗ اﺮﺑ .

ي ﺎﻘﻣ ﯾ ﺛﺎﺗ ﻪﺴ ﯿ ود ﻪﻧﺎﮔاﺪﺟ ﺮ

ﺰﺠﺗ نﻮﻣزآ زا ﻞﻣﺎﻋ ﯾ

راو ﻪ ﯾ ﺲﻧﺎ ﯾ اﺮﺑ ،ﻪﻓﺮﻃ ﮏ ي

ﺎﻘﻣ ﯾ نﻮﻣزآ زا ماﻮﺗ ﺮﺛا ﻪﺴ ﺰﺠﺗ

ﯾ راو ﻪ ﯾ ود ﺲﻧﺎ

ﺎﻘﻣ و ﻪﻓﺮﻃ ﯾ

ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﻦ ﮐﻮﺗ نﻮﻣزآ زا ﺎﻫ ﯽ

لﺎﻤﺘﺣا ﺢﻄﺳ رد 5

دﺮﮔ هدﺎﻔﺘﺳا ﺪﺻرد ﯾ

ﺪ ﺎﺘﻧ. ﯾ ﺞ

ا زا ﻞﺻﺎﺣ ﯾ

ﻘﺤﺗ ﻦ ﯿ ﺑ ﻪﮐ داد نﺎﺸﻧ ﻖ ﯿ

اﺰﻓا ﻦ ﯾ ﻨﭽﻤﻫ و لﺎﻌﻓ لﺎﻏز ﺖﻈﻠﻏ ﺶ ﯿ

نزُا نﺎﻣز تﺪﻣ ﻦ

ﻫد ﯽ ﻟﻮﺗﺎﭘ راﺪﻘﻣ ﺶﻫﺎﮐ ﺎﺑ ﯿ

ﻘﺘﺴﻣ ﻪﻄﺑار ﻦ ﯿ

دراد دﻮﺟو ﻢ ﺑ ﯿ.

ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ناﺰ ﯿ

رد ﻦ

ﺖﻈﻠﻏ 2 /0 و لﺎﻌﻓ لﺎﻏز مﺮﮔ 9

ﻗد ﯿ ﻫد نزُا ﻪﻘ ﯽ

ﻨﭽﻤﻫ ،ﺪﻣآ ﺖﺳد ﻪﺑ ﯿ

ﺳرﺮﺑ رد ﻦ ﯽ

ماﻮﺗ ﺮﺛا

ﺖﻈﻠﻏ ﺎﻫ ي نﺎﻣز و لﺎﻌﻓ لﺎﻏز ﺎﻫ

ي ﻫد نزُا ﯽ ﺎﺘﻧ ﯾ ﺑ داد نﺎﺸﻧ ﺞ ﯿ

ﻐﺘﻣ ود ﻦ ﯿ دﻮﺟو ﻞﺑﺎﻘﺘﻣ ﺮﺛا ﺮ

نﺎﻣز رد و دراد 8

ﻗد ﯿ ﻫد نزُا ﻪﻘ ﯽ

ﺖﻈﻠﻏ ﺎﺑ 5

/1 ﺑ لﺎﻌﻓ لﺎﻏز مﺮﮔ ﯿ

ﺮﺘﺸ ﯾ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ﻦ ﯿ

ﻦ

دﺮﮔ هﺪﻫﺎﺸﻣ ﯾ

ﺪ ﺳرﺮﺑ. ﯽ ﮔرﺎﮐ ﻪﺑ ﺎﺑ داد نﺎﺸﻧ ﺮﺿﺎﺣ ﯿﺮ

ي ﻣ لﺎﻌﻓ لﺎﻏز هاﺮﻤﻫ ﻪﺑ نزُا زﺎﮔ ﯽ

رد ناﻮﺗ

ﻟﻮﺗ تﺎﺠﻧﺎﺧرﺎﮐ ﯿ

ﻣ بآ ﺪ ﯿ ﻣ هﺮﺘﻧﺎﺴﻨﮐ و هﻮ ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﻣﺎﮔ و داد ﺶﻫﺎﮐ ار ﻦ ﯽ

ﺖﻣﻼﺳ رد ﺮﺛﻮﻣ

ﻣ بآ تاردﺎﺻ و ﻪﻌﻣﺎﺟ ﯿ

ﺖﺷادﺮﺑ هﺮﺘﻧﺎﺴﻨﮐ و هﻮ .

ﻪﻠﺠﻣ

مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ

www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ

ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ

(2)

ﺸﺑ نﺎﺸﻟا ﯿﺮ ي نارﺎﮑﻤﻫ و ﺛﺄﺗ

ﯿ ﻟﻮﺗﺎﭘ ﺮﺑ لﺎﻌﻓ لﺎﻏذ و نزا ﺮ ﯿ

ﺳ هﺮﺘﻧﺎﺴﻨﮐرد ﻦ ﯿ

ﺐ

1 - ﻪﻣﺪﻘﻣ

ﺎﻨﺻ ﯾ اﺬﻏ ﻊ ﯾ ﯽ ﯾ ﮑ ﯽ ﺶﺨﺑ زا ﺎﻫ ي رﻮﻀﺣ ﺎﺑ ﻂﺒﺗﺮﻣ هﺪﻤﻋ

ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﻣ ﺎﻫ ﯽ ﺪﺷﺎﺑ ﻨﻣا ﻪﭼ ﺮﮔا . ﯿ

اﺬﻏ ﺖ ﯾ ﯽ هﺪﻤﻋ رﻮﻃ ﻪﺑ

دﻮﺒﻬﺑ ﯾ ﭘ ﺎﻣا ﺖﺳا ﻪﺘﻓﺎ ﯿ

ﺖﻓﺮﺸ ﻣز رد ﺎﻫ ﯿ

تاﺮﺛا ﻪﻨ

ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي ﺷﺎﻧ ﯽ اﺬﻏ داﻮﻣ زا ﯾ

ﯽ ﮔدﻮﻟآ زا ﻞﺻﺎﺣ ﯽ

ﻣﯿ ﺑوﺮﮑ ﯽ ﺷ ، ﯿﻤ ﯿﺎ ﯾ ﯽ ﺴﮐﻮﺗ و ﯿ ﻦ ﺴﺑ رد ﺎﻫ ﯿ

رﺎ ي ﺎﻫرﻮﺸﮐ زا

ﯾ ﻧ ﺖﺧاﻮﻨﮑ ﯿ

ﺖﺴ ﯾ. ﮑ ﯽ ﮔدﻮﻟآ زا ﯽ ﺎﻫ ي ﺎﻨﺻ ﻢﻬﻣ ﯾ ﺷﻮﻧ ﻊ ﯿ ﻧﺪ ﯽ و ﺎﻫ

ﻣ ﻦﯿﻟﻮﺗﺎﭘ ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ هﺮﺘﻧﺎﺴﻨﮐ ﯽ

ﻪﻧﻮﮔ ﻪﻠﯿﺳو ﻪﺑ ﻪﮐ ﺪﺷﺎﺑ يﺎﻫ

ﮏﭙﮐ زا ﻒﻠﺘﺨﻣ دﻮﺷ ﯽﻣ ﺪﯿﻟﻮﺗ ﺎﻫ

] 1[

چرﺎﻗ ﻦﯾا ﺮﺜﮐا . ﮏﭙﮐ و

ﺎﻫ

هﻮﯿﻣ يور ﻮﻠﻫ و رﻮﮕﻧا ،ﯽﺑﻼﮔ ،ﺐﯿﺳ هﮋﯾو ﻪﺑ هﺪﺷ ﺪﺳﺎﻓ يﺎﻫ

ﯽﻣ ﺪﺷر هزاﺪﻧا ﺪﻨﻨﮐ ظﺎﺤﻟ ﻦﯾا زا ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ ﻦﯾا راﺪﻘﻣ يﺮﯿﮔ

ﯽﻣ ﻪﮐ ﺖﺳا ﻢﻬﻣ ﺋﻮﺳ ﺮﺛا ﺪﻧاﻮﺗ

ﺪﺷﺎﺑ ﻪﺘﺷاد نﺎﺴﻧا ﺖﻣﻼﺳ ﺮﺑ ﯽ

] 2 [ ﺳ بآ هﺮﺘﻧﺎﺴﻨﮐ ﺖﻌﻨﺻ . ﯿ

ﺐ ﯾ ﮑ ﯽ ﺎﻨﺻ زا ﯾ اﺬﻏ ﻢﻬﻣ ﻊ ﯾ

ﯽ رد

ﺎﺟ تاردﺎﺻ ﺮﻈﻧ ﻪﻄﻘﻧ زا و هدﻮﺑ رﻮﺸﮐ ﯾ

و هﺎﮕ ﯾ هﮋ ا ي دﻮﺧ ﻪﺑ ار

ﺖﺳا هداد صﺎﺼﺘﺧا ﯾ.

ﮑ ﯽ ﺳﺎﺳا تﻼﮑﺸﻣ زا ﯽ

ﺮﺳ ﺮﺑ دﻮﺟﻮﻣ

ﺳ بآ هﺮﺘﻧﺎﺴﻨﮐ تاردﺎﺻ هار ﯿ

ﻣ ﺐ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﺖﺳا نآ رد ﻦ .ا

ﯾ ﻦ

ﺎﻣ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ اد ﻦ ار ي ﺻﺎﺧ ﯿ ﻧژﺎﺗﻮﻣ ﺖ ﯿ اﺰﻧﺎﻃﺮﺳ و ﮏ ﯾ

ﯽ ﻪﺑ و هدﻮﺑ

ﻤﻫ ﯿ ﻧﺎﻬﺟ دراﺪﻧﺎﺘﺳا ﺖﻠﻋ ﻦ ﯽ

ﺳ بآ هﺮﺘﻧﺎﺴﻨﮐ رد نآ راﺪﻘﻣ ﯿ

ﺐ

ﻣ و دوﺪﺤﻣ ﯿ

ار نآ ناﺰ 50 ppb ﻌﺗ ﯿﯿ ﺖﺳا هﺪﺷ ﻦ ]

3 [.

ﻪﻧﺎﻔﺳﺎﺘﻣ

ﻟﻮﺗﺎﭘ راﺪﻘﻣ ﯿ

هﺮﺘﻧﺎﺴﻨﮐ تﺎﺠﻧﺎﺧرﺎﮐ تﻻﻮﺼﺤﻣ ﺮﺜﮐا رد دﻮﺟﻮﻣ ﻦ

ﺳ بآ ﯿ ا رد ﺐ ﯾ ﺑ ناﺮ ﯿ ﻣ نآ دراﺪﻧﺎﺘﺳا راﺪﻘﻣ زا ﺶ ﯽ

ﺪﺷﺎﺑ ا ﯾ. ﻦ

ﮐﺮﺗ ﯿ ﻤﺳ ﺐ ﯽ ﺳو ﻪﺑ ﯿ ﻃ ﻪﻠ ﯿ هدﺮﺘﺴﮔ ﻒ ا

ي چرﺎﻗ زا ﺪﻨﻧﺎﻣ ﺎﻫ ﻨﭘ ﯽ

ﺳﯿ ﻠﯿ مﻮﺴﻧﺎﭙﺴﮐا مﻮ ﻨﭘ و

ﯽ ﺳ ﯿﻠﯿ مﻮﻟﻮﺗﺎﭘ مﻮ ور ﻪﮐ

ي ﺧﺮﺑ ﯽ زا

ﻣﯿ هﻮ ﺳ ﻪﻠﻤﺟزا ﺎﻫ ﯿ

ﺤﻄﺳ ﺖﻓﺎﺑ ﻪﮐ ﺐ ﯽ

نآ ﺳآ ﺎﻫ ﯿ د ﺐ ﯾ و هﺪ

ا ﻪﺑ هدﻮـﻟآ ﯾ

چرﺎﻗ ﻦ ﻣ ﺎﻫ ﯽ ﻟﻮﺗ ﺪـﻨﺷﺎﺑ ﯿ ﻣ ﺪـ ﯽ ا زا و ددﺮﮔ ﯾ

ﺮﻃ ﻦ ﯾ ﻖ

ﻣ بآ ﻪﺑ ﯿ ﭘ هار هﻮـ ﯿ اﺪـ ﻣ ﯽ ﺪﻨﻨﮐ ﭘ ﻦﯿﻟﻮﺗﺎ لﺎﺳرد 1970 ﻂﺳﻮﺗ

ﺲﻧاژآ ﺑﯿ ﻦ ﻠﻠﻤﻟا ﯽ ﻘﺤﺗ ﯿ تﺎﻘ نﺎﻃﺮﺳ ﻪﺑ ناﻮﻨﻋ ﯾ ﮏ

"

هدﺎﻣ

اﺰﻧﺎﻃﺮﺳ ي ﻟﺎﻤﺘﺣا ﯽ اﺮﺑ ي نﺎﺴﻧا

"

ﻪﻘﺒﻃ ﺪﻨﺑ ي ﺪﺷ . اﯾ ﻦ ﯾ ﻪﺘﻓﺎ

ﻧاﺮﮕﻧ ﯽ ﺳو ﯿﻌ ﯽ ار رد ﺢﻄﺳ ﺑﯿ ﻦ ﻠﻠﻤﻟا ﯽ رد ﭘ ﯽ ﺖﺷاد ] 4 [.

دﺪﻌﺘﻣ تﺎﻌﻟﺎﻄﻣ ي

ﺎﻣزآ ﺢﻄﺳ رد ﯾ

ﻫﺎﮕﺸ ﯽ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ﺖﻬﺟ ﯿ

ﻦ

ﺳ بآ هﺮﺘﻧﺎﺴﻨﮐ رد ﯿ

شور زا هدﺎﻔﺘﺳا ﺎﺑ ﺐ ﺎﻫ

ي ﺪﻨﻧﺎﻣ ﻒﻠﺘﺨﻣ

آﺮﻓ ،ﺎﻣﺎﮔ ﻪﻌﺷا زا هدﺎﻔﺘﺳا ﯾ

ﺗراﺮﺣ ﺪﻨ ﯽ ﻤﺨﺗ ، ﯿ روآﺮﻓ و ﺮ ي

ﻟوا ﯿ ﻪ

ﺎﺘﻧ و ﻪﺘﻓﺮﮔ ترﻮﺻ نﻮﻨﮐﺎﺗ ﯾ

ﺑﻮﻠﻄﻣ ناﺪﻨﭼ ﺞ ﯽ

ا زا ﯾ ﻘﺤﺗ ﻦ ﯿ تﺎﻘ

ﺖﺳا هﺪﺸﻧ ﻞﺻﺎﺣ ]

2[

ﺳرﺮﺑ . ﯽ زﺎﮔ دﺮﺑرﺎﮐ ﻪﮐ ﺪﻧداد نﺎﺸﻧ ﺎﻫ

ﺴﺑ ﺶﻘﻧ ﺐﯿﺳ بآ رد ﻦﯿﻟﻮﺗﺎﭘ ناﺰﯿﻣ ﺶﻫﺎﮐ ياﺮﺑ نزُا ﯿ

ﻤﻬﻣ رﺎ ﯽ

اﯾ ﻣ ﺎﻔ ﯽ اﺮﺑ نزُا زا هدﺎﻔﺘﺳا ﺪﻨﮐ ي

ﻠﻘﺗ ﯿ ﺴﮐﻮﺗﻼﻓآ ﻞ ﯿ

و ترذ رد ﻦ

ﺮﺛﻮﻣ رﻮﻃ ﻪﺑ ﻪﻧاد ﻪﺒﻨﭘ ﻪﻟﺎﺠﻨﮐ ي

ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ .

اﯾ ﻨﭽﻤﻫ شور ﻦ ﯿ

اﺮﺑ ﻦ ي ﻣ ﺶﻫﺎﮐ ﯿ و مﻮﻤﺳ ناﺰ ﻣ

ﯿ ﺴﮐﻮﺘ ﯿ و ﻦ

ﻟﻮﻧﻮﻣ ﯽ ﻣﻮﻓ ﯿ ﻧ ﻦ ﯿ ﺖﺳا هدﻮﺑ ﺮﺛﻮﻣ ﺰ .

ﻘﻣ زا هدﺎﻔﺘﺳا ﯿ

ﺎﻣزآ سﺎ ﯾ ﺸ ﯽ اﺮﺑ نزُا

ي ﺰﺠﺗ ﯾ اﺮﮐا مﻮﻤﺳ ﻪ ﮐﻮﺗ

ﺴ ﯿ ﻦ

، A ﻟﻮﺗﺎﭘ ﯿ ز و ﻦ ﯾ ﻟاﺮ ﯿ ﻧ نﻮﻔ ﯿ ﺰ

ﻘﻣ رد ﯿ ﺎﻣزآ سﺎ ﯾ ﻫﺎﮕﺸ ﯽ ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﺮﺛﻮﻣ هدﺎﻔﺘﺳا درﻮﻣ ]9

-

5 [ ﻨﭽﻤﻫ . ﯿ ﺎﺘﻧ ﻦ ﯾ ﺳرﺮﺑ ﺞ ﯽ ﻣ لﺎﻌﻓ لﺎﻏز ﻪﮐ هداد نﺎﺸﻧ ﺎﻫ ﯽ

ﺪﻧاﻮﺗ

ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ رد ﯿ

ددﺮﮔ ﻊﻗاو ﺮﺛﻮﻣ ﻦ ]

10 و 11 .[

ﻤﻫ رد اﺬﻟ ﯿ ﻣ ،ﺎﺘﺳار ﻦ ﯿ

ﺎﻣ ناﺰ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ ﻟﻮﺗﺎﭘ ﻦ ﯿ ﻪﻧﻮﻤﻧ رد ﻦ ﺎﻫ

ي

ﺳو ﻪﺑ نآ ﺶﻫﺎﮐ و ﺐ ﯿ

درﻮﻣ لﺎﻌﻓ لﺎﻏز و نزُا ﻪﻠ

زرا ﯾﺑﺎ ﯽ ﺖﻓﺮﮔ راﺮﻗ .

2

- شور و داﻮﻣ ﺎﻫ

2 - 1 - داﻮﻣ

ﺳ بآ ﯿ ﻠﻐﺗ ﺐ ﯿ لﺎﺳ ﻞﺼﻓ ﺮﺧآ لﻮﺼﺤﻣ زا هﺪﺷ ﻆ 1397

ود زا

ﻟﻮﺗ ﻪﻧﺎﺧرﺎﮐ ﯿ

ﺳ بآ هﺪﻨﻨﮐ ﺪ ﯿ

ﻣورا رد ﺐ ﯿﻪ

ﻨﭽﻤﻫ و ﯿ ﻪﻧﻮﻤﻧ ﻦ

ﮕﻧﺎﺧ ﯽ ﻬﺗ ﯿ ﺳ زا هﺪﺷ ﻪ ﯿ

ﺎﻬﺒ ي ﻨﭘ چرﺎﻗ ﻪﺑ هدﻮﻟآ ﯿ

ﺴ ﯿﻠ ﯿ

،مﻮ

ژﺮﭙﺳآ ﯾ

،سﻮﻠ ﺑﯿ ﻣﻼﮐﻮﺴ ﯿ ﻤﺴ ﯽ ﺪﻨﺷﺎﺑ ﻬﺗ ﯿ ﺎﻣد رد و ﻪ ي

ﯾ لﺎﭽﺨ

راﺪﻬﮕﻧ ي ﺪﺷ ﺷ داﻮﻣ . ﯿﻤ ﯿﺎ ﯾ ﯽ ا رد هدﺎﻔﺘﺳا درﻮﻣ ﯾ

ﻞﻣﺎﺷ حﺮﻃ ﻦ

ﺗاﯿ ﻞ تﺎﻨﺑﺮﮐ ،تﺎﺘﺳا ﺪﺳ

ﯾ ﺳا ،ﻢ ﯿﺪ ﺘﺳا ﯿ ﺳﻼﮔ ﮏ ﯿ ،لﺎﻌﻓ ﻦﺑﺮﮐ ،لﺎ

ﻟﻮﺗﺎﭘ ﯿ ﻧﻮﺘﺳا و دراﺪﻧﺎﺘﺳا ﻦ ﯿ

ﺮﺘ ﯾ ﻞ ) ﻟﻮﺗ ﯿ كﺮﻣ ﺖﮐﺮﺷ ﺪ (

اراد ي

ﺰﺠﺗ ﻪﺟرد ﯾﻪ

ا ي دﻮﺑ هزاﺪﻧا مﺎﺠﻧا ﺖﻬﺟ . ﮔﯿ

ﺮ ي ﺳ بآ ﻪﻧﻮﻤﻧ ﯿ

ﺐ

ﻠﻐﺗ ﯿ ﺮﺑ ﻪﺑ ﺮﻄﻘﻣ بآ ﺎﺑ هﺪﺷ ﻆ ﯾ

ﺲﮑ 12 ﻗر ﯿ اﺮﺑ و ﻖ ي ﻠﮐ ﯿ ﻪ

هزاﺪﻧا ﮔﯿ ﺮ ي ﻬﺗ ﻪﻧﻮﻤﻧ زا ﺎﻫ ﯿ

دﺮﮔ هدﺎﻔﺘﺳا هﺪﺷ ﻪ ﯾﺪ

.

2 - 2 - شور ﺎﻫ

ﺎﻫرﻮﺘﮐﺎﻓ ي ﺎﻣزآ درﻮﻣ ﯾ

ﺛﺎﺗ ﻞﻣﺎﺷ ﺶ ﯿ

ﻫد نزُا و لﺎﻌﻓ ﻦﺑﺮﮐ ﺮ ﯽ

ﺮﺑ

ﻣﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﺳرﺮﺑ درﻮﻣ ﻢﻫ ﺎﺑ و ﺎﻬﻨﺗ ترﻮﺻ ﻪﺑ ﻦ ﯽ

ﺖﻓﺮﮔ راﺮﻗ

نﺎﻣز ﻪﻄﺑار اﺪﺘﺑا ﺎﻫ

ي ﻫد نزُا ﻒﻠﺘﺨﻣ ﯽ

زﺎﮔ رﺎﺸﻓ ﺎﺑ ﯾ

و نﺎﺴﮑ

ﻨﭽﻤﻫ ﯿ دﺎﻘﻣ ﻦ ﯾ ور ار لﺎﻌﻓ ﻦﺑﺮﮐ ﻒﻠﺘﺨﻣ ﺮ ي

ﻣ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ بآ ﻦ

ﺳ ﯿ ﻫد نزُا و لﺎﻌﻓ لﺎﻏز ماﻮﺗ ﺮﺛا ﺲﭙﺳ ،ﺪﺷ ﻪﺒﺳﺎﺤﻣ ﺐ ﯽ

زرا ﯾﺑﺎ ﯽ دﺮﮔ ﯾ ﺎﻣزآ ﺪ ﯾ ﻫﺎﮕﺸﻧاد دﺎﻬﺟ رد تﺎﺸ ﯽ

- ﻣورا هﺎﮕﺸﻧاد ﯿ

ﻪ

دﺮﮔ مﺎﺠﻧا ﯾﺪ .

2 - 2 - 1 - جاﺮﺨﺘﺳا ﺟ زﺎﺳاﺪ ي ﻟﻮﺗﺎﭘ ﯿ ﻦ

اﺮﺑ ي ﻟﻮﺗﺎﭘ جاﺮﺨﺘﺳا ﯿ

ﺳﺮﺗ و ﻦ ﯿ ﻨﺤﻨﻣ ﻢ ﯽ ﻟﺎﮐ ﯿ ﺳاﺮﺒ ﯿ اﺪﺘﺑا ،نآ نﻮ

10 ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ﺮﻄﻘﻣ بآ ﺎﺑ

ﭘ ﯿ دراو ﺖﭙ ﯾ ﻗ ﮏ ﯿ ﻤﺗ رﻮﺘﻧﺎﮐد ﻒ ﯿ

ﺪﺷ ﺰ

ﺲﭙﺳ و 10 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﻟﻮﺗﺎﭘ دراﺪﻧﺎﺘﺳا لﻮﻠﺤﻣ زا ﺮﺘ ﯿ

ﻦ ) 20mg/l (

اﺮﺑﺎﻨﺑ ،ﺪﺷ ﻪﻓﺎﺿا نآ ﻪﺑ ﯾ

ﻟﻮﺗﺎﭘ ﺖﻈﻠﻏ ﻦ ﯿ

ﻞﺻﺎﺣ لﻮﻠﺤﻣ رد ﻦ

20 ﻣﯿ ﻟ ﺮﺑ مﺮﮔوﺮﮑ ﯿ

ﺪﺷ ﺮﺘ . ﺳو ﻪﺑ ﺲﭙﺳ ﯿ

ﻪﻠ ﯾ ﭘ ﮏ ﯿ ،ﺖﭙ 10 ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ

ﺗاﯿ ﻞ ﻗ ﻪﺑ ﺺﻟﺎﺧ تﺎﺘﺳا ﯿ

تﺪﻣ ﻪﺑ و ﻪﻓﺎﺿا رﻮﺘﻧﺎﮐد ﻒ 2

ﻗد ﯿ ﻪﻘ

زﺎﻓ ﺎﺗ ﺪﺷ ﺎﻫر دﻮﺧ لﺎﺣ ﻪﺑ رﻮﺘﻧﺎﮐد ﺪﻌﺑ و ﺪﺷ هدز ﻢﻬﺑ ﻢﻫ زا ﺎﻫ

ﺪﻧﻮﺷ اﺪﺟ ﺎﭘ زﺎﻓ ،ﺎﻫزﺎﻓ نﺪﺷ اﺪﺟ زا ﺲﭘ .

ﯾﯿ ﻦ ) ﺑآ زﺎﻓ ﯽ (

زﺎﺳاﺪﺟ ي ور و ي ﻻﺎﺑ زﺎﻓ ﯾ ﯽ ) ﺗا زﺎﻓ ﯿ ﻞ تﺎﺘﺳا ( ، 1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ

ﻣ تﺎﻨﺑﺮﮐ لﻮﻠﺤ ﺪﺳ

ﯾ ﻢ 5/

1 تﺪﻣ ﻪﺑ رﻮﺘﻧﺎﮐد و ﻪﻓﺎﺿا ﺪﺻرد 15

(3)

ﻧﺎﺛ ﯿ ﺑﻮﺧ ﻪﺑ ﻪ ﯽ ﻧﺎﺘﺤﺗ زﺎﻓ ادﺪﺠﻣ و ﺪﺷ هدز ﻢﻬﺑ ﯽ

دﺮﮔ اﺪﺟ ﯾﺪ .

ﺲﭙﺳ 1 ﻣ ﯿﻠ ﯽ ﻟﯿ هرﺎﺑود و ﻪﻓﺎﺿا رﻮﺘﻧﺎﮐد ﻪﺑ ﺮﻄﻘﻣ بآ ﺮﺘ تﺪﻣ ﻪﺑ

15 ﻧﺎﺛ ﯿ دﺮﮔ ﺎﻫر دﻮﺧ لﺎﺣ ﻪﺑ طﻮﻠﺨﻣ و ﺪﺷ هدز ﻢﻫ ﻪ ﯾ

ﺎﻫزﺎﻓ ﺎﺗ ﺪ

د ﻢﻫ زا ﯾ دﻮﺷ اﺪﺟ ﺮﮕ ﺑآ زﺎﻓ زا ﺲﭘ .

ﯽ

، ﻟآ زﺎﻓ ﯽ ) ﺗاﯿ ﻞ تﺎﺘﺳا ( رد

ﻦﻟرا ﻞﺧاد 25

ﻣ ﯿﻠ ﯽ ﻟﯿ ﻤﺗ ﺮﺘ ﯿ ر ﮏﺸﺧ و ﺰ ﯾ

ﺪﺷ ﻪﺘﺨ زا ﺲﭘ .

ﺳا هﺮﻄﻗ ود ندوﺰﻓا ﯿ

ﺘﺳا ﺪ ﯿ ﺳﻼﮔ ﮏ ﯿ رد ﻦﻟرا ،لﻮﻠﺤﻣ ﻪﺑ لﺎ

بآ مﺎﻤﺣ 40

ﺴﻠﺳ ﻪﺟرد ﯿ

ﺳو ﻪﺑ و ﺪﺷ هداد راﺮﻗ سﻮ ﯿ

ﺮﺟ ﻪﻠ ﯾ نﺎ

ﻧ زﺎﮔ ﯿ ﺗا ،نژوﺮﺘ ﯿ ﻞ ﺨﺒﺗ ﻼﻣﺎﮐ تﺎﺘﺳا ﯿ

دﺮﮔ ﺮ ﯾﺪ 2] و 12 .[

2 - 2 - 2 - ﮔ هزاﺪﻧا ﯿﺮ ي ﻟﻮﺗﺎﭘ ﯿ ﻦ

اﺮﺑ ي ﮔ هزاﺪﻧا ﯿﺮ ي ﻟﻮﺗﺎﭘ ﯿ ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ شور زا ﻦ ﯽ

ﺎﻣ ﯾ ﺎﺑ ﻊ

ارﺎﮐ ﯾ ﯽ ﻻﺎﺑ ) لﺪﻣ CECIL نﻮﺘﺳ زا ،نﺎﺘﺴﻠﮕﻧا ﺖﺧﺎﺳ C18

) ﺖﺑﺎﺛ زﺎﻓ تارذ ﺮﻄﻗ هزاﺪﻧا 5

ﻣﯿ وﺮﮑ ﺮﺘﻣ ( لﻮﻃ ﻪﺑ 25 ﺘﻧﺎﺳ ﯽ ﺮﺘﻣ و

ﻠﺧاد ﺮﻄﻗ ﯽ 6 /4 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ نﻮﺘﺳ ﻆﻓﺎﺤﻣزا و لﻮﻃ ﻪﺑ C18

1 ﺘﻧﺎﺳ ﯽ ﺮﺘﻣ ﻠﺧاد ﺮﻄﻗ و ﯽ

6 /4 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( هدﺎﻔﺘﺳا ﺪﺷ . كﺮﺤﺘﻣ زﺎﻓ

ﻧﻮﺘﺳا ﯿ ﺮﺘ ﯾ ﻞ - ﺮﻄﻘﻣ بآ ) 5/

95 - (V\V نآ ﺖﮐﺮﺣ ﺖﻋﺮﺳ و ﯾ

ﮏ

ﻣﯿ ﻠ ﯽ ﻟﯿ ﻗد رد ﺮﺘ ﯿ

زﺎﺳرﺎﮑﺷآ زا و ﻪﻘ جﻮﻣ لﻮﻃ رد UV

276 ﺮﺘﻣﻮﻧﺎﻧ ﺪﺷ هدﺎﻔﺘﺳا ﺳﺎﺴﺣ .

ﯿ هﺎﮕﺘﺳد ﺖ رد HPLC

005 /0

ﻈﻨﺗ ﯿ دﺮﮔ ﻢ ﯾ رﺰﺗ ﻪﻘﻠﺣ ﻢﺠﺣ و ﺪ ﯾ

ﻧ هدﺎﻔﺘﺳا درﻮﻣ ﻖ ﯿ

ﺰ 20

ﻣﯿ ﻟوﺮﮑ ﯿ دﻮﺑ ﺮﺘ زﺎﺳاﺪﺟ. ي ور ي ﺳدﺎﺘﮐا نﻮﺘﺳ ﯿ

ﻞ ﺳ ﯿ نﻼ ) لﻮﻃ ﻪﺑ

25 ﺘﻧﺎﺳ ﯽ ﻠﺧاد ﺮﻄﻗ ،ﺮﺘﻣ ﯽ

6 /4 ﻣ ﯿﻠ ﯽ هزاﺪﻧا و ﺮﺘﻣ ي

تارذ 5

ﻣﯿ ﺮﺘﻣوﺮﮑ ZORBAX Eclipse XDB (

ﻧﺎﭙﻤﮐ ﺖﺧﺎﺳ ﯽ

Dr. Mainsch ﺪﺷ مﺎﺠﻧا نﺎﻤﻟآ

راﺰﻓا مﺮﻧ زا .

Chemstation هداد شزادﺮﭘ ﺖﻬﺟ

ﺎﻫ هدﺎﻔﺘﺳا ﺪﺷ ﻪﺑ رﻮﻈﻨﻣ

زﺎﺳاﺪﺟ ي ﺖﺒﺴﻧ ﺎﺑ كﺮﺤﺘﻣ زﺎﻓ زا 5

ﻧﻮﺘﺳا ﺪﺻرد ﯿ

ﺮﺘ ﯾ و ﻞ 95

ﻮﻠﻓ ﺎﺑ بآ ﺪﺻرد ﯾﯿ

ﻣ ﮏ ﯿﻠ ﯽ ﻟﯿ ﻗد ﺮﺑ ﺮﺘ ﯿ ﺪﺷ هدﺎﻔﺘﺳا ﻪﻘ نﺎﻣز .

زﺎﺳاﺪﺟ ي 9 ﻗد ﯿ دﻮﺑ ﻪﻘ .

اﺮﺑ ي هزاﺪﻧا ﮔﯿ ﺮ ي ﺳو ﻪﺑ ؛ ﯿ ﻪﻠ ﯾ ﭘ ﮏ ﯿ

،هژوژ ﺖﭙ 1

ﻣﯿ ﻠ ﯽ ﻟﯿ بآ ﺮﺘ

ﺎﺣ ﻦﻟرا ﻞﺧاد رد ﺮﻄﻘﻣ ي

ﻬﺗ هرﺎﺼﻋ ﯿ

بﻮﺧ و ﺪﺷ ﻪﻓﺎﺿا هﺪﺷ ﻪ

ﻟﻮﺗﺎﭘ ﺎﺗ ﺪﺷ هدز ﻢﻬﺑ ﯿ

زا هدﺎﻔﺘﺳا ﺎﺑ ﺲﭙﺳ و ددﺮﮔ ﻞﺣ نآ رد ﻦ

صﻮﺼﺨﻣ ﮓﻧﺮﺳ هﺎﮕﺘﺳد

) HPLC ﻣﺎﻫ ﯿ نﻮﺘﻠ ( هﺎﮕﺘﺳد ﻪﺑ ﻪﻧﻮﻤﻧ

رﺰﺗ HPLC ﯾ ﭘ ﺢﻄﺳ و هﺪﺷ ﻖ ﯿ

زا هﺪﻣآ ﺖﺳد ﻪﺑ ﮏ

ماﺮﮔﻮﺗﺎﻣوﺮﮐ ﯾ

دﺮﮔ ﺖﺷاددﺎ ﯾﺪ

ﻤﻫ . ﯿ ﺎﻣزآ ﻦ ﯾ ﺎﺑ ﺶ 15 ، 20 و 25

ﻣﯿ ﻟوﺮﮑ ﯿ ﻟﻮﺗﺎﭘ دراﺪﻧﺎﺘﺳا لﻮﻠﺤﻣ ﺮﺘ ﯿ

ﻦ ) 20mg/l ( رﺎﺑ ﺮﻫ و راﺮﮑﺗ

ز ﺢﻄﺳ ﯾ ﭘ ﺮ ﯿ ماﺮﮔﻮﺗﺎﻣوﺮﮐ زا ﻞﺻﺎﺣ ﮏ ﯾ

ﺪﺷ ﺖﺷاددﺎ اﺮﺑ .

ي

هزاﺪﻧا ﮔﯿ ﺮ ي ﻘﻣ ﻟﻮﺗﺎﭘ راﺪ ﯿ ﻪﻧﻮﻤﻧ رد ﻦ ﺎﻫ

ي ﺳ بآ ﯿ ﺮﮐذ شور ،ﺐ

ﺎﺟ ﻪﺑ و راﺮﮑﺗ هﺪﺷ ي

ﺳ بآ زا ﺮﻄﻘﻣ بآ ﯿ

دﺮﮔ هدﺎﻔﺘﺳا ﺐ ﯾ

و ﺪ

ﻟﻮﺗﺎﭘ راﺪﻘﻣ ﯿ

ور زا ﻦ ي

ﻨﺤﻨﻣ ﯽ ﻟﺎﮐ ﯿ ﺳاﺮﺒ ﯿ ﺪﺷ ﻪﺒﺳﺎﺤﻣ نﻮ .

زﺎﺳاﺪﺟ ي ردﻮﭘ لﺎﻌﻓ ﻦﺑﺮﮐ تارذ ي

ﺳ بآ زا ﯿ ﻠﮑﺸﻣ رﺎﮐ ﺐ ﯽ

ﻓﺎﺻ ﺬﻏﺎﮐ زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﮐ ﺖﺳا ﯽ

ﻓﺎﺻ و ﻦﻤﺗاو ﯽ

ﮐ ﯿ ارﻮﮔ لﺰ ﯾ ﻦ ﺮﺑ ﻞﮑﺸﻣ

ﺪﺷ فﺮﻃ 2]

و 12 .[

2 - 2 - 3 - زرا شور ﯾﺑﺎ

ﯽ ﺛﺎﺗ ﯿ ﻣ ﺮﺑ لﺎﻌﻓ لﺎﻏز و نزُا ﺮ ﯿ

ناﺰ

ﻟﻮﺗﺎﭘ ﯿ ﻪﻧﺎﮔاﺪﺟ ترﻮﺻ ﻪﺑ ﻦ

اﺮﺑ ي زرا ﯾﺑﺎ ﯽ ﺛﺎﺗ ﯿ ﻪﻧﻮﻤﻨﻬﺳ ﻪﻧﺎﮔاﺪﺟ ترﻮﺼﺑ لﺎﻌﻓ لﺎﻏز و نزُا ﺮ

ﻞﻣﺎﺷ ﺐ : ﻪﻧﻮﻤﻧ 1 ﻪﻧﻮﻤﻧ ، 2 ﻬﺗ ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ و ﯿ

ﻪ

دﺮﮔ ﯾ راﺪﻘﻣ ﻪﺑ ﻪﻧﻮﻤﻧ ﺮﻫ زا ﺪ 50

ﺳ ﯽ ﺳ ﯽ رد و ﻪﺘﺷادﺮﺑ

ﺷ ﯿ ﻪﺸ ﺎﻫ ي ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ ) ﺎﻌﻤﺟ 27 ﺷ ﯿ ﻪﮐ ﻪﺸ 12 ﺷ ﯿ طﻮﺑﺮﻣ ﻪﺸ

ﺛﺎﺗ ﻪﺑ ﯿ دﺎﻘﻣ ﺮ ﯾ لﺎﻌﻓ لﺎﻏز ﺮ 05

/0 ، 1/

0 ، 15 /0 و 2/

0 و مﺮﮔ 12

ﺷ ﯿ نﺎﻣز ﻪﺑ طﻮﺑﺮﻣ ﻪﺸ ﺎﻫ

ي ﻫد نزُا ﻒﻠﺘﺨﻣ ﯽ

3 ، 5 ، 7 و 9 ﻗد ﯿ ﻪﻘ

و 3 ﺷ ﯿ ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ﻪﺸ (

ﺗ ﺮﻫ ﯿ ﺪﺷ مﺎﺠﻧا راﺮﮑﺗ ﻪﺳ رد رﺎﻤ لﺎﻏز .

لﺎﻌﻓ ﺳﻮﺑ ﯿ وزاﺮﺗ ﻪﻠ ي د ﯾ ﺠ ﯿ زﻮﺗ ﺖﻗد ﺎﺑ لﺎﺘ ﯾ

ﺎﻫ ﻪﻧﻮﻤﻧ ﻪﺑ و ﻦ ي

ﺷ ﯿ دﺮﮔ ﻪﻓﺎﺿا ﻪﺸ ﯾ

ﻪﻧﻮﻤﻧ طﻼﺘﺧا ﻞﻤﻋ ﺪ ﺎﻫ

ي ﺳ بآ ﯿ ﻦﺑﺮﮐ ﺎﺑ ﺐ

ﺳو ﻪﺑ لﺎﻌﻓ ﯿ

ﻃﺎﻨﻐﻣ نز ﻢﻬﺑ ﻪﻠ ﯿ

ﺴ ﯽ ﺖﻋﺮﺳ ﺎﺑ و ﯾ

اﺮﺑ نﺎﺴﮑ ي ﻠﮐ ﯿ ﻪ

ﻪﻧﻮﻤﻧ تﺪﻣ ﻪﺑ ﺎﻫ 10

ﻗد ﯿ ﻨﭽﻤﻫ ﺖﻓﺮﮔ مﺎﺠﻧا ﻪﻘ ﯿ

ﻫد نزُا ﻦ ﯽ ﻪﺑ

ﺳو ﯿ رﺎﺸﻓ ﺎﺑ ﻞﺧاد ﺖﺧﺎﺳ زﺎﺳ نزُا هﺎﮕﺘﺳد ﻪﻠ ﯾ

رﺎﻬﭼ رد نﺎﺴﮑ

ﺳو ﻪﺑ نﺎﻣز ﯿ

ﺷ ﻪﻠ ﯿ ﻪﻧﻮﻤﻧ زﺎﮔ لﺎﻘﺘﻧا ﮓﻨﻠ ﻫد زﺎﮔ ﺎﻫ

ﯽ ﺪﻧﺪﺷ

ﻓﯿ ﺳاﺮﺘﻠ ﯿ ﻪﻧﻮﻤﻧ ﻞﮐ نﻮ ﺳو ﻪﺑ ﺎﻫ

ﯿ ﻓﺎﺻ ﺬﻏﺎﮐ ﻪﻠ ﯽ

هرﺎﻤﺷ ﺎﺑ ﻦﻤﺗاو

860 ﮐ و ﯿ رﻮﮔﺰ ) ﻓﺎﺻ ناﻮﻨﻋ ﻪﺑ ﯽ

ﮑﻤﮐ ﯽ ( ﺬﭘ ترﻮﺻ ﯾ

ﺖﻓﺮ . و

اﺮﺑ ي ﮔ هزاﺪﻧا ﯿﺮ ي ﻣ ﯿ ﻗﺎﺑ ناﺰ ﯽ ﻟﻮﺗﺎﭘ هﺪﻧﺎﻣ ﯿ

ﺳا و ﻦ ﯿ ﺪ ﯾ ﻪﺑ ﻪﺘ

ﺎﻣزآ ﯾ هﺎﮕﺸ ﺷ هداد لﺎﻘﺘﻧا HPLC

دﺎﻘﻣ ﺖﺳا ﺮﮐذ ﻪﺑ مزﻻ ﺪ ﯾ

ﺮ

ﻟﻮﺗﺎﭘ ﯿ ﺎﻣزا ﻞﺒﻗ ﻦ ﯾ

ﺶ ﻧ ﯿ ﻌﺗ ﺰ ﯿﯿ دﺮﮔ ﻦ ﯾﺪ .

2 - 2 - 4 - زرا شور ﯾﺑﺎ

ﯽ ﺛﺎﺗ ﯿ ﺮـﺑ لﺎﻌﻓ لﺎﻏز و نزُا ماﻮﺗ ﺮ

ﻟﻮﺗﺎﭘ ﯿ ﻦ لﺎﻌﻓ لﺎﻏز ﺖﻈﻠﻏ رﺎﻬﭼ لﺎﻌﻓ لﺎﻏز و نزُا ماﻮﺗ ﺮﺛا مﺎﺠﻧا ﺖﻬﺟ

ﻞﻣﺎﺷ 0 ، 5/

0 ، 1 و 5/

1 نﺎﻣز رﺎﻬﭼ ﻮﻣﺮﮔ 0

، 3 ، 5 و 8 ﻗد ﯿ ﻪﻘ

ﻪﻧﻮﻤﻧ اﺪﺘﺑا و بﺎﺨﺘﻧا ﺗ لﺎﻌﻓ لﺎﻏز ﺎﺑ ﺎﻫ

ﯿ ﻫد نزُا ﺲﭙﺳ ورﺎﻤ ﯽ

ﺳو ﻪﺑ ﻪﻧﻮﻤﻧ مﺎﻤﺗ ﺲﭙﺳ ﺖﻓﺮﮔ مﺎﺠﻧا ﯿ

ﻓﺎﺻ ﺬﻏﺎﮐ ﻪﻠ ﯽ

ﺎﺑ ﻦﻤﺗاو

هرﺎﻤﺷ 860 ﮐ و ﯿ رﻮﮔﺰ ) ﻓﺎﺻ ناﻮﻨﻋ ﻪﺑ ﯽ

ﮑﻤﮐ ﯽ ( ﻓﯿ ﻪﻤﻫ ﺪﻧﺪﺷ ﺮﺘﻠ

ﺎﻣزآ ﯾ ﺖﻓﺮﮔ ترﻮﺻ راﺮﮑﺗ ﻪﺳ رد تﺎﺸ ﻪﻧﻮﻤﻧ و .

اﺮﺑ ﺎﻫ ي هزاﺪﻧا

ﮔﯿ ﺮ ي ﻣ ﯿ ﻗﺎﺑ ناﺰ ﯽ ﻟﻮﺗﺎﭘ هﺪﻧﺎﻣ ﯿ

و ﻦ ﺎﻣزآ ﻪﺑ pH

ﯾ هﺎﮕﺸ HPLC

ﺘﻧا ﺪﺷ هداد لﺎﻘ .

2 - 3 - شور هداد ﻞﯿﻠﺤﺗ و ﻪﯾﺰﺠﺗ راﺰﺑا و ﺎﻫ ﺎﻫ

اﺮﺑ ي زرا ﯾﺑﺎ ﯽ ﺖﻈﻠﻏ ﻪﻄﺑار ﺎﻫ

ي ﻫد نزُا و لﺎﻌﻓ لﺎﻏز ﻒﻠﺘﺨﻣ ﯽ

ﻣ ﺎﺑ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﺳﺮﮔر زا ﻦ ﯿ

ﻄﺧ هدﺎﺳ نﻮ ﯽ

اﺮﺑ و ي ﺎﻘﻣ ﯾ ﻪﺴ ي

ﻣﯿ ﮕﻧﺎ ﯿ ﺛﺎﺗ ﻦ ﯿ ﺗ ﺎﺑ نزُا و لﺎﻌﻓ لﺎﻏز ﺮ ﯿ

ﺰﺠﺗ زا ﺪﻫﺎﺷ رﺎﻤ ﯾ

راو ﻪ ﯾ ﺲﻧﺎ

ﯾ اﻮﻧآ ﻪﻓﺮﻃ ﮏ )

One Way ANOVA (

ﺳرﺮﺑ و ﯽ زا ماﻮﺗ ﺮﺛا

ﺰﺠﺗ ﯾ راو ﻪ ﯾ اﻮﻧآ ﻪﻓﺮﻃ ود ﺲﻧﺎ )

Two Way ANOVA (

و

ﺎﻘﻣ ﯾ ﻪﺴ ي ﻣ ﯿ ﮕﻧﺎ ﯿ ﻤﮑﺗ نﻮﻣزآ ﻂﺳﻮﺗ ﻦ ﯿﻠ

ﯽ ﮐﻮﺗ ﯽ لﺎﻤﺘﺣا ﺢﻄﺳ رد

(4)

ﺐ

95 ﺳو ﻪﺑ ﺪﺻرد ﯿ

ﻪﻠ ي راﺰﻓا مﺮﻧ ) (SPSS ﺪﺷ مﺎﺠﻧا .

3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ

3 - 1 - ﮕﺘﺴـﺒﻤﻫ و ﻪﻄﺑار ﯽ

ـﺑ ﯿ نﺎـﻣز ﻦ ﺎـﻫ

ي نزُا

ﻫد ﯽ ﻣ و ﯿ ناﺰ pH

ﺳﺮﮔر ﻪﻄﺑار ﯿ

ﻧﻮ ﯽ ﺑﯿ نﺎﻣز ﻦ ﺎﻫ ي نزُا ﻫد ﯽ ﻣ و ﯿ ناﺰ ﻪﺳ pH

ﺐ ﻪﻧﻮﻤﻧ ﻞﻣﺎﺷ 1،2

ﺮﺿ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ،ﺪﻫﺎﺷ و ﯾ

ﺐ

ﺒﺗﯿ ﯿ ﻦ ) R² ( هﺮﺘﻧﺎﺴﻨﮐ ﻪﻧﻮﻤﻧ رد ﻪﮐ داد نﺎﺸﻧ 2

ﺑ ﯿ ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ناﺰ

ﺮﺿ ﯾ ﺒﺗ ﺐ ﯿﯿ دراد دﻮﺟو ﻦ )

ﻞﮑﺷ 1 ( .

Fig 1 Regression relationship between time of ozonation (min) and pH of 1, 2, and controlsample

3 - 2 - ﮕﺘﺴﺒﻤﻫ و ﻪﻄﺑار ﯽ

ﺑ ﯿ ﺖﻈﻠﻏ ﻦ ﺎﻫ

ي لﺎﻏز

ﻣ و لﺎﻌﻓ ﯿ

ناﺰ pH

ﻧﻮ ﯽ ﺑ ﯿ ﺖﻈﻠﻏ ﻦ ﺎﻫ ي ﻣ و لﺎﻌﻓ لﺎﻏز ﯿ

ناﺰ ﻪﺳ pH ﺳ هﺮﺘﻧﺎﺴﻨﮐ

ﯿ ﻪﻧﻮﻤﻧ ﻞﻣﺎﺸﺒ 1،2

ﺮﺿ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ،ﺪﻫﺎﺷ و ﯾ

ﺒﺗ ﺐ ﯿﯿ ﻦ

) R² ( ﺑ ﺪﻫﺎﺷ هﺮﺘﻧﺎﺴﻨﮐ رد ﻪﮐ داد نﺎﺸﻧ ﯿ

ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ﺮﺿ ناﺰ ﯾ ﺐ

ﺒﺗﯿ ﯿ دراد دﻮﺟو ﻦ )

ﻞﮑﺷ 2 ( .

Fig 2 Regression relationship between activated carbon (gr) and pH of 1, 2, and control samples

(5)

3 - 3 - ﮕﺘﺴــﺒﻤﻫ و ﻪــﻄﺑار ﯽ

ــﺑ ﯿ نﺎــﻣز ﻦ ﺎــﻫ

ي

نزُا ﻫد ﯽ ﻣ و ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ

ﻦ

ﻧﻮ ﯽ ﺑ ﯿ نﺎﻣز ﻦ ﺎﻫ ي نزُا ﻫد ﯽ ﻣ و ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﻪﺳ ﻦ ﺳ هﺮﺘﻧﺎﺴﻨﮐ

ﯿ ﻪﻧﻮﻤﻧ ﺐ 1،2

ﺮﺿ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ،ﺪﻫﺎﺷو ﯾ

) R² ( هﺮﺘﻧﺎﺴﻨﮐ ﻪﻧﻮﻤﻧ رد ﻪﮐ داد نﺎﺸﻧ 2

ﺑ ﯿ ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ﺮﺿ ناﺰ ﯾ ﺐ

ﻞﮑﺷ 3 ( .

Fig 3 Regression relationship between time of ozonation (min) and patulin content (ppb) in 1, 2 and control samples

3 - 4 - ﮕﺘﺴﺒﻤﻫ و ﻪﻄﺑار ﯽ

ﺑ ﯿ ﺖﻈﻠﻏ ﻦ ﺎﻫ

ي لﺎﻏز

ﻣ و لﺎﻌﻓ ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ

ﻦ

ﻧﻮ ﯽ ﺑ ﯿ ﺖﻈﻠﻏ ﻦ ﺎﻫ ي ﻣ و لﺎﻌﻓ لﺎﻏز ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﻦ ﺳ هﺮﺘﻧﺎﺴﻨﮐ ﻪﺳ

ﯿ ﻪﻧﻮﻤﻧ ﺐ 1،2 ﺮﺿ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ،ﺪﻫﺎﺷو ﯾ

) R² ( هﺮﺘﻧﺎﺴﻨﮐ ﻪﻧﻮﻤﻧ رد ﻪﮐ داد نﺎﺸﻧ 1

ﺑ ﯿ ﺮﺘﺸ ﯾ ﻣ ﻦ ﯿ ﺮﺿ ناﺰ ﯾ ﺐ

ﻞﮑﺷ 4 ( .

Fig 4 Regression relationship between activated carbon (min) and patulin content (ppb)of 1, 2, and control samples

(6)

ﺐ

3 - 5 - ﺰﺠﺗ ﯾ راو ﻪ ﯾ نﺎﻣز ﺲﻧﺎ ﺎﻫ

ي نزُا ﻫد ﯽ ور ي

هﺮﺘﻧﺎﺴﻨﮐ ﺎﻫ

ﺰﺠﺗ ﯾ راو ﻪ ﯾ نﺎﻣز ﺲﻧﺎ ﺎﻫ

ي ) 0 ، 3 ، 5 ، 7 و 9 ﻗد ﯿ ﻪﻘ ( نزُا ﻫد ﯽ

ور ي ﻪﻧﻮﻤﻧ هﺮﺘﻧﺎﺴﻨﮐ ﻪﺳ 1،2

ﺎﻘﻣ ﺖﻬﺟ رد ﺪﻫﺎﺷو ﯾ

ﺛﺎﺗ ﻪﺴ ﯿ ور ﺮ ي ﻟﻮﺗﺎﭘ

ﯿ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ داد نﺎﺸﻧ دﻮﺟﻮﻣ ﻦ زا ﻪﮐ هﺪﺷ ﻪﺒﺳﺎﺤﻣ F

F

گرﺰﺑ لوﺪﺟ و هدﻮﺑ ﺮﺗ

زا هﺪﺷ ﻪﺒﺳﺎﺤﻣ P 5

ﮏﭼﻮﮐ ﺪﺻرد ﺮﺗ

ﻣ ﯽ ﻨﻌﻣ فﻼﺘﺧا ؛ﺪﺷﺎﺑ ﯽ

راد ي ﺑ ﯿ نﺎﻣز ﻦ ﺎﻫ ي نزُا ﻫد ﯽ دﻮﺟو

دراد ) لوﺪﺟ 1 ( .

Table 1 Analysis of variance ozonation times on patulin contentinof 1, 2, and control samples

Sample Source

Sums of squares Degree of

freedom Mean of

squares F

P

Treatment 40143.16

4 10035.79

Error 68.72

10

6.87 1

Total 40211.89

14 1460.32**

0.001

Treatment 62460.65

4 15615.16

Error 553.42

10

55.34 2

Total 63014.08

14 282.15**

0.001

Treatment 59615.13

4 14903.78

Error 242.00

10

24.20 Control

Total 59857.13

14 615.85**

0.001

3 - 6 - ﺎﻘﻣ ﯾ ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﺗ ﻦ ﯿ رﺎﻤ ﺎﻫ

ﺎﻘﻣ ﯾ ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﺗ ﻦ ﯿ رﺎﻤ ﮐﻮﺗ نﻮﻣزآ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ ﯽ

ﺢﻄﺳ رد

لﺎﻤﺘﺣا 95 ﻞﮑﺷ ﻖﺑﺎﻄﻣ ﺪﺻرد 5

ﻪﺑ ﺪﻣآ ﺖﺳد ) ﻞﮑﺷ 5 ( .

Fig 5 Comparison of average ozonation times on three concentrates using Tukey test at 95%

probability level. The comparison of the three concentrates is independent of each other.The columns with similar letters are not significantly

different from each other.

**Significance at 99% probability level ﺎﻘﻣ و ﻞﮑﺷ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯾ

ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﺗ ﻦ ﯿ رﺎﻤ اﺰﻓا ﺎﺑ ،ﺎﻫ ﯾ نﺎﻣز ﺶ ﺎﻫ ي

نزُا ﻫد ﯽ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ﯿ

دﺮﮔ هﺪﻫﺎﺸﻣ ﻦ ﯾ

نزُا و ﺪ ﻫد ﯽ رد 9 ﻗد ﯿ ﻪﻘ

ﺑﯿ ﺮﺘﺸ ﯾ ﻣ رد ﺶﻫﺎﮐ ﻦ ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ هﺮﺘﻧﺎﺴﻨﮐ ﻪﺳ ﺮﻫ رد ار ﻦ

ﻪﻧﻮﻤﻧ 1،2 ﺖﺷاد ﺪﻫﺎﺷ و .

3 - 7 - ﻟﺎﻧآ ﯿ راو ﺰ ﯾ ﺲﻧﺎ ﺛﺎﺗ ﯿ ﺖـﻈﻠﻏﺮ ﺎـﻫ

ي لﺎـﻏز

ور لﺎﻌﻓ ي

هﺮﺘﻧﺎﺴﻨﮐ ﺎﻫ

ﺰﺠﺗ ﯾ راو ﻪ ﯾ ﺛﺎﺗ ﺲﻧﺎ ﯿ ﺖﻈﻠﻏ ﺮ ﺎﻫ ي لﺎﻌﻓ لﺎﻏز )

05 /0 ، 1/

0 ، 15 /0 ،

2/

0 و 0 مﺮﮔ ( ور ي ﻪﻧﻮﻤﻧ هﺮﺘﻧﺎﺴﻨﮐ ﻪﺳ 1،2

ﺖﻬﺟ رد ﺪﻫﺎﺷ و

ﺎﻘﻣ ﯾ ﺛﺎﺗ ﻪﺴ ﯿ ور ﺮ ي ﻟﻮﺗﺎﭘ ﯿ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ داد نﺎﺸﻧ دﻮﺟﻮﻣ ﻦ F

زا ﻪﮐ هﺪﺷ ﻪﺒﺳﺎﺤﻣ گرﺰﺑ لوﺪﺟ F

و هدﻮﺑ ﺮﺗ زا هﺪﺷ ﻪﺒﺳﺎﺤﻣ P

5 ﮏﭼﻮﮐ ﺪﺻرد ﻣ ﺮﺗ

ﯽ ﻨﻌﻣ فﻼﺘﺧا ؛ﺪﺷﺎﺑ ﯽ

راد ي ﺑ ﯿ نﺎﻣز ﻦ ﺎﻫ ي

نزُا ﻫد ﯽ دراد دﻮﺟو )

لوﺪﺟ 2 ( .

Table 2 Analysis of variance activated carbon concentration on patulin content of 1, 2, and control samples

Sample Source

freedom Mean of squares

F P

Treatment 43150.80

4 10787.70

Error 101.32

10

10.13 1

Total 43252.12

14 1064.68**

0.001

Treatment 54280.90

4 13570.22

Error 69.72

10

6.97 2

Total 54350.63

14 1946.18**

0.001

Treatment 92729.82

4 23182.45

Error 126.22

10

12.62 Control

Total 92856.05

14 1836.59**

0.001

**Significance at 99% probability level

(7)

ﺢﻄﺳ رد

Fig 6 Comparison of the average concentrations of activated carbon on patulin content of three sample

using Tukey test at 95% probability level. The comparison of the three concentrates is independent

of each other; the columns with similar letters are not significantly different from each other.

ﺎﻘﻣ و ﻞﮑﺷ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯾ

ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﺗ ﻦ ﯿ رﺎﻤ ﺎﺘﻧ ،ﺎﻫ ﯾ داد نﺎﺸﻧ ﺞ :

ﺎﺑ

اﺰﻓا ﯾ ﺖﻈﻠﻏ ﺶ ﺎﻫ

ي ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ لﺎﻌﻓ لﺎﻏز ﯿ

دﺮﮔ هﺪﻫﺎﺸﻣ ﻦ ﯾ

و ﺪ

ﺖﻈﻠﻏ ﺎﺑ لﺎﻌﻓ لﺎﻏز 2/

0 ﺑ ﯿ ﺮﺘﺸ ﯾ ﻣ رد ﺶﻫﺎﮐ ﻦ ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ ار ﻦ ﻪﻧﻮﻤﻧ

1،2 ﺖﺷاد ﺪﻫﺎﺷ و .

3 - 9 - ﺎﻘﻣ ﯾ ﻫد نزُا شور ود ﻪﺴ ﯽ

لﺎﻌﻓ لﺎﻏز و

ور ي ﻣ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ

ﻪﻧﻮﻤﻧ ﻦ 1،2

ﺪﻫﺎﺷو

ﺎﻘﻣ ﯾ ﻫد نزُا شور ود ﻪﺴ ﯽ

ور لﺎﻌﻓ لﺎﻏز و ي

ﻣ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﻦ

ﻪﻧﻮﻤﻧ 1،2 ﻼﺘﺧا ﺮﺜﮐاﺪﺣ ﺖﻈﻠﻏ و نﺎﻣز رد داد نﺎﺸﻧ ﺪﻫﺎﺷو ف

ﻨﻌﻣ ﯽ راد ي دراﺪﻧ دﻮﺟو شور ود ﺎﺑ ﺶﻫﺎﮐ رد )

ﻞﮑﺷ 7 (

) لوﺪﺟ 3 (.

3 - 10 - ﻃﻮﻠﺨﻣ ﺮﺛا نزُﺎ

ﻫد ﯽ ﺮﺑ لﺎﻌﻓ لﺎﻏز و

ﻣﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ

ﻦ

ﺰﺠﺗ ﯾ راو ﻪ ﯾ ﺛﺎﺗ ﺲﻧﺎ ﯿ ﺖﻈﻠﻏ ﺮ 15 /0 نﺎﻣز و لﺎﻌﻓ لﺎﻏز 7

ﻗد ﯿ ﻪﻘ

نزُا ﻫد ﯽ ور ماﻮﺗ و اﺪﺟ ﺖﻟﺎﺣ رد ي

ﻣ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ هﺮﺘﻧﺎﺴﻨﮐ ﻪﺳ ﻦ

ﻪﻧﻮﻤﻧ 1،2 ﺎﺑ داد نﺎﺸﻧ ﺪﻫﺎﺷو ﻪﺑ ﻪﺟﻮﺗ

زا ﻪﮐ هﺪﺷ ﻪﺒﺳﺎﺤﻣ F F

گرﺰﺑ لوﺪﺟ و هدﻮﺑ ﺮﺗ

زا هﺪﺷ ﻪﺒﺳﺎﺤﻣ P 5

ﮏﭼﻮﮐ ﺪﺻرد ﺮﺗ

ﻣ ﯽ ﻨﻌﻣ فﻼﺘﺧا ؛ﺪﺷﺎﺑ ﯽ

راد ي ﺑ ﯿ نﺎﻣز ﻦ ﺎﻫ ي نزُا ﻫد ﯽ دﻮﺟو

دراد ) لوﺪﺟ 4 ( .

Table 3 T-test analysis of two methods of ozonation time and activated charcoalconcentration on the amount of patulin in 1, 2 and control sample

Sample Treatments

SE

± Mean t(df)

P

Ozonation 58.25

± 187.30

Activated charcoal 1 52.81

± 163.55 )

3 ( 1.29 0.287

Ozonation 65.26

± 183.68

Activated charcoal 2 54.24

± 176.95 )

3 ( 0.57 0.504

Ozonation 62.32

± 237.74

Control Activated charcoal

74.51

± 206.88 )

3 ( 2.36 0.099

Table 4 Analysis of variance of 0.15 activated charcoal concentration and time of 7 minutes ozonation in the isolated and mixing form on the amount of patulin with three concentrates of sample

1, sample 2 and control

Sample Source

freedom Mean of

squares F

P

Treatment 18838.12

2 9419.06

Error 3693.10

6

615.51 1

Total 22531.22

8 15.30**

0.001

Treatment 8234.73

2 4117.36

Error 88.15

6

14.69 2

Total 8322.89

8 280.22**

0.001

Treatment 12356.27

2 6178.13

Error 80.35

6

13.39 Control

Total 12436.62

8 461.32**

0.001

ﺢﻄﺳ رد

(8)

ﻪﻠﺠﻣ مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ

هرﺎﻤﺷ

3 12

، هرود 19

، درا ﯾ ﺖﺸﻬﺒ 1401

Fig 7 Comparison of the mean effect of activated charcoal in concentration 0.15 grand 7 min ozonation time in isolated and combined state on

the amount of patulin of three concentrates of sample 1, sample 2 and control sample using Tukey

test at 95% probability level. The comparison of the three concentrates is independent of each other,

the columns with similar letters are not significantly different from each other.

د ﺳرﺮﺑ ر ﯽ ﺛﺎﺗ ﯿ ﺖﻈﻠﻏﺮ 15 /0 نﺎﻣز و لﺎﻌﻓ لﺎﻏز 7

ﻗد ﯿ نزُا ﻪﻘ -

ﻫد ﯽ ور ماﻮﺗ و اﺪﺟ ﺖﻟﺎﺣ رد ي

ﻣ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ هﺮﺘﻧﺎﺴﻨﮐ ﻪﺳ ﻦ

ﻪﻧﻮﻤﻧ 1،2 ﺎﺘﻧ ،ﺪﻫﺎﺷو ﯾ

داد نﺎﺸﻧ ﺞ :

نزُا ماﻮﺗ دﺮﺑرﺎﮐ ﻫد

ﯽ لﺎﻏز و

ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ رد لﺎﻌﻓ ﯿ

نآ ﮏﺗ ﮏﺗ دﺮﺑرﺎﮐ ﻪﺑ ﺖﺒﺴﻧ ﻦ ﺛﺎﺗ ﺎﻫ

ﯿ ﺮ

ﺑﯿ ﺮﺘﺸ ي دراد .

3 - 12 - ﺳرﺮﺑ ﯽ اﻮﺗ ﺮﺛا رد نزُا و لﺎﻌﻓ لﺎﻏز م

ﺖﻈﻠﻏ نﺎﻣز و ﺎﻫ ي ﻒﻠﺘﺨﻣ

ﺳرﺮﺑ ﯽ ﺰﺠﺗ ﯾ راو ﻪ ﯾ ﺛﺎﺗ زا ﻞﺻﺎﺣ ﺲﻧﺎ ﯿ

رد لﺎﻌﻓ لﺎﻏز ماﻮﺗ ﺮ

ﺖﻈﻠﻏ ﺎﻫ ي ) 0 ، 5/

0 ، 1 و 5/

1 مﺮﮔ ( نﺎﻣز و ﺎﻫ ي ﻫد نزُا ﯽ ) 0 ،

3 ، 5 و 8 ﻗد ﯿ ﻪﻘ ( لوﺪﺟ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ داد نﺎﺸﻧ 5

ﻨﻌﻣ فﻼﺘﺧا ﯽ

راد ي ﺑ ﯿ ﺖﻈﻠﻏ ﻦ ﻨﭽﻤﻫ و ﻞﻣﺎﻋ ود ﺮﻫ رد ﺎﻫ ﯿ

اﻮﺗ ﺮﺛا ﻦ نآ م ﺎﻫ

دراد دﻮﺟو )

لوﺪﺟ 5 ( .

Table 5 Analysis of variance obtained from the combined effect of activated carbon and ozone at different concentrations

Source Sums of

squares Degree

of freedom Mean of

squares F

P Eta index

Activated carbon 106878.68

3 35626.13

82.803**

0.001 0.886

Ozone 51300.56

3 17100.18

39.745**

0.001 0.788

Activated carbon

× Ozone 12877.35

9 1430.81

3.32**

0.006 0.483

Error 13768.00

32 430.25

Total 510041.00

48

3 - 13 - ﺎﻘﻣ ﯾ ﻣ ﻪﺴ ﯿ ﮕﻧﺎ ﯿ ﺗ ﻦ ﯿ ﺎﻫرﺎﻤ

ﺳرﺮﺑ رد ﯽ ﺖﻈﻠﻏ رد ﻞﺑﺎﻘﺘﻣ ﺮﺛا ﺎﻫ

ي و لﺎﻌﻓ لﺎﻏز ﻒﻠﺘﺨﻣ

نﺎﻣز ﺎﻫ ي ﻫد نزُا ﯽ ﺑ ﯿ ﺗ ﻦ ﯿ ﻨﻌﻣ فﻼﺘﺧا ﺎﻫرﺎﻤ ﯽ

راد ي د ﯾ ﺪﺷ هﺪ

ﺑﯿ ﺮﺘﺸ ﯾ ﺖﻈﻠﻏ ﻂﺳﻮﺗ ﺶﻫﺎﮐ ﻦ ﺎﻫ

ي طﻮﺑﺮﻣ لﺎﻌﻓ لﺎﻏز ﻒﻠﺘﺨﻣ

ﺖﻈﻠﻏ ﻪﺑ 5/

1 ﺑ و مﺮﮔ ﯿ ﺮﺘﺸ ﯾ ﻫد نزُا رد ﺶﻫﺎﮐ ﻦ ﯽ

ﻪﺑ طﻮﺑﺮﻣ 8

ﻗد ﯿ ﻫد نزُا ﻪﻘ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ ) ﻞﮑﺷ 8 ، 9 و 10 ( .

ﺳرﺮﺑ رد ﯽ ﺖﻈﻠﻏ ماﻮﺗ ﺮﺛا ﺎﻫ

ي نﺎﻣز و لﺎﻌﻓ لﺎﻏز ﺎﻫ

ي ﻫد نزُا ﯽ

ﺎﺘﻧ ﯾ نﺎﻣز رد داد نﺎﺸﻧ ﺞ 8

ﻗد ﯿ ﻫد نزُا ﻪﻘ ﯽ

ﺖﻈﻠﻏ ﺎﺑ 5/

1 مﺮﮔ

ﺑ لﺎﻌﻓ لﺎﻏز ﯿ

ﺮﺘﺸ ﯾ ﺶﻫﺎﮐ ﻦ ﻟﻮﺗﺎﭘ ﯿ دﺮﮔ هﺪﻫﺎﺸﻣ ﻦ ﯾﺪ

.

Fig 8 Comparison of mean reduction of patulin by

different concentrations of activated carbon by Tukey test. Treatments that have similar letters do

not differ significantly.

(9)

Fig 9 Comparison of mean reduction patulin by ozonation times by Tukey test. Treatments that have similar letters do not differ significantly.

Fig 10 Interaction of activated carbon concentrations and ozonation times on reduction

patulin

4 - ﺘﻧ ﯿ ﮔ ﻪﺠ ﯿﺮ ي ﻠﮐ ﯽ

هزاﺪﻧا ﮔ ﯿﺮ ي و ﻌﺗ ﯿﯿ ﻦ راﺪﻘﻣ ﻗد ﯿ ﻖ ﻟﻮﺗﺎﭘ ﯿ ﻦ و شﻼﺗ رد ﺖﻬﺟ

ﺶﻫﺎﮐ راﺪﻘﻣ نآ رد بآ ﺳ ﯿ ﺐ رد ﺖﻬﺟ ﻣﺎﺗ ﯿ ﺖﻣﻼﺳ ﻦ فﺮﺼﻣ

هﺪﻨﻨﮐ و ﻧﯿ ﺰ دﻮﺒﻬﺑ ﻌﺿو ﯿ ﺖ تاردﺎﺻ اﯾ ﻦ لﻮﺼﺤﻣ ﺰﺋﺎﺣ ﻤﻫا ﯿ ﺖ

ﻣ ﯽ ﺪﺷﺎﺑ . سﺎﻧو لﺎﮐادﺎﮐ 2002)

( ﻢﺳ صاﻮﺧ ادز

ﯾ ﯽ لﺎﻌﻓ لﺎﻏز

ور ار ي ﻟﻮﺗﺎﭘ ﯿ ﺳ بآ ﻦ ﯿ

ﺳرﺮﺑ ﺐ ﯽ ﺎﺘﻧ و ﯾ ﻪﮐ ،داد نﺎﺸﻧ ﺞ

نﺎﻣز ﺎﻫ ي ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ رد لﺎﻌﻓ لﺎﻏز زا هدﺎﻔﺘﺳا ﻒﻠﺘﺨﻣ ﯿ

و ﻦ

ﻫﯿ ﺴﮐورﺪ ﯽ ﺘﻣ ﯿ ﺴﺑ ﺶﻘﻧ لارﻮﻓرﻮﻓ ﻞ ﯿ

ﻤﻬﻣ رﺎ ﯽ ﺮﺘﻬﺑ و ،ﺖﺷاد ﯾ

ﻦ

ﺘﻧﯿ رد ﻪﺠ 3 ﻟ رد مﺮﮔ ﯿ تﺪﻣ ﻪﺑ ﺮﺘ 5

ﻗد ﯿ ﻪﻘ دروآ ﺖﺳد ﻪﺑ ]

13 [.

ﺳرﺮﺑ رد ﯽ ﻧ ﺮﺿﺎﺣ ﯿ ﺰ 2/

0 رد مﺮﮔ 50 ﺳ ﯽ ﺳ ﯽ لدﺎﻌﻣ 4 رد مﺮﮔ

ﻟﯿ رد ﺮﺘ 10 ﻗد ﯿ ﺑ نﺎﻣز ﻪﻘ ﯿ ﺮﺘﺸ ﯾ ادز ﻢﺳ ﻦ ﯾ

ﯽ ﺎﺑ ﻪﮐ داد نﺎﺸﻧ ار

ﺎﺘﻧ ﯾ ا ﺞ ﯾ ﻘﺤﺗ ﻦ ﯿ دوﺪﺣ ﺎﺗ ﻖ ي

ﻣ ﻖﺒﻄﻨﻣ ﯽ ﺪﺷﺎﺑ ﺳرﺮﺑ رد . ﯽ و ﺮﻨﺑﺎﻫ

نارﺎﮑﻤﻫ )

2000 ( ور رد ي زﻮﭙﻣﺎﮐ زا هدﺎﻔﺘﺳا ﯾ

ﺎﺑ ﻦﺑﺮﮐ ﺖ

ﺻﺎﺧ ﯿ ﺑﺬﺟ ﺖ ﯽ ﺎﺘﻧ ﯾ ﺞ ا ﻪﮐ ،داد نﺎﺸﻧ ﯾ

ﮐﺮﺗ ﻦ ﯿ ﺻﺎﺧ ﺐ ﯿ ﻻﺎﺑ ﺖ ﯾ ﯽ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ رد

ﯿ دراد ﻦ ] 14 [ ﺳرﺮﺑ رد . ﯽ نارﺎﮑﻤﻫ و ﻦﻤﮑٌﮔ

ور ي ﺛﺎﺗ ﯿ ﻀﻌﺑ ﺮ ﯽ ﮐﺮﺗ زا ﯿ ار تﺎﺒ ﯾ ﻢﺳ رد ﺞ ادز

ﯾ ﯽ ﺳ بآ ﯿ زا ﺐ

ﻟﻮﺗﺎﭘ ﯿ ﺎﺘﻧ ﻦ ﯾ ﺗﻻژ ،داد نﺎﺸﻧ ﺞ ﯿ

ﻧﻮﺘﻨﺑ ،ﻦ ﯿ ﺛﺎﺗ لﺎﻌﻓ لﺎﻏز و ﺖ ﯿ

ﺮ

ﺴﺑ ﯿ ﺘﺒﺜﻣ رﺎ ﯽ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ رد ﯿ

ﺪﻨﺘﺷاد ﻦ ]

10 [ ﻘﺤﺗ رد . ﯿ ﺮﺿﺎﺣ ﻖ

ﻧﯿ ادز ﻢﺳ و ﻪﺑﺎﺸﻣ ﺮﺛا لﺎﻌﻓ لﺎﻏز ﺰ ﯾ

ﯽ ا ﯾ دﺮﮐ دﺎﺠ ﺎﺘﻧ. ﯾ ﺶﻫوﮋﭘ ﺞ

دﯾ نارﺎﮑﻤﻫ و ﻮ )

2019 ( ﺳرﺮﺑ رد ﯽ ﺛﺎﺗ ﯿ ور نزُا ﺮ ي ﻣ ﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﻦ

ﮐﺮﺗ و ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯿ ﻣ ﻪﮐداد نﺎﺸﻧ ﮏ ﯿ

ﻟﻮﺗﺎﭘ ناﺰ ﯿ زا ﻦ 06 / 201 ز ﻪﺑ ﯾ ﺮ

50 ﻣ ﯿﻠ ﯽ ﻟ ﺮﺑ مﺮﮔ ﯿ تﺪﻣ رد ﺮﺘ 15

ﻗد ﯿ ﺶﻫﺎﮐ ﻪﻘ ﯾ

ﺖﻓﺎ ﻨﭽﻤﻫ . ﯿ ﻦ

ﻣﯿ ﮐﺮﺗ ناﺰ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯿ ﺎﺗ ﮏ 36 / 75 ﺶﻫﺎﮐ ﺪﺻرد ﯾ

ﺖﻓﺎ ] 6[

رد .

ﻘﺤﺗ ﯿ ﻧ ﺮﺿﺎﺣ ﻖ ﯿ

ﻣ ﺰ ﯿ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ناﺰ ﯿ

ﻫد نزُا نﺎﻣز ﺎﺑ ﻦ ﯽ

ﮕﺘﺴﺒﻤﻫ ﯽ ﻮﻣ ﻪﮐ داد نﺎﺸﻧ ﺖﺒﺜﻣ ﯾ

ﺻﺎﺧ ﺪ ﯿ ادز ﻢﺳ ﺖ ﯾ

ﯽ نزُا

ﻣ ﯽ ﺪﺷﺎﺑ ﺳرﺮﺑ رد . ﯽ ﺎﺠﻧا ي نارﺎﮑﻤﻫ ) 2018 ( ور ي صاﻮﺧ

ﻢﺳ ادز ﯾ ﯽ ﺰﺠﺗ و ﯾ ﻟﻮﺗﺎﭘ ﻪ ﯿ ور رد ﻦ ي ﺳ ﯿ ﭘاﺮﺗ نزُا ،داد نﺎﺸﻧ ﺐ ﯽ

ور ي ﭘ ﺶﻫﺎﮐ ﻟﻮﺗﺎ ﯿ ﺴﺑ ﺶﻘﻧ ﻦ ﯿ

ﺘﺒﺜﻣ رﺎ ﯽ ا ﯾ ﻣ ﺎﻔ ﯽ ﻫ و ﺪﻨﮐ ﯿ ﺛﺎﺗ ﭻ ﯿ ﺮ

ﻮﺳ ﯾ ﯽ ور ي ﮐ ﯿﻔ ﯿ ﻤﻧ نآ ﺖ ﯽ دراﺬﮔ ] 15 [ وﺪﻟﺎﺗﺎﮐ . ) 2008 ( نﺎﺸﻧ

ﻣ نزُا ﻪﮐ ،داد ﯿ

ﻟﻮﺗﺎﭘ ﺪﻧاﻮﺘ ﯿ ﺑﻼﮔ بآ ﻦ ﯽ

ﺳ و ﯿ ﻨﻌﻣ رﻮﻃ ﻪﺑ ار ﺐ ﯽ

راد ي ﺪﻫد ﺶﻫﺎﮐ ]

9[

ﺳرﺮﺑ رد . ﯽ ﻧ ﺮﺿﺎﺣ ﯿ ﭘاﺮﺗ نزُا ﺰ ﯽ

رد

ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ﯿ

ﺑﻮﻠﻄﻣ ﺮﺛا ﻦ ﯽ

داد نﺎﺸﻧ ﺳرﺮﺑ رد .

ﯽ و ﺎﺟارﺎﮐ

ﻟو ﯽ ﻮﻠﻏوا ) 2009 ( ور ي ﺛﺎﺗ ﯿ ور نزُا ﺮ ي

ﻣ ﺶﻫﺎﮐ ﯿ

ناﺰ

ﺎﻣ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ ﻟﻮﺗﺎﭘ ﻦ ﯿ ﺎﺘﻧ ﻦ ﯾ ﻘﺘﺴﻣ ﻪﻄﺑار ،داد نﺎﺸﻧ ﺞ ﯿﻤ

ﯽ ﺑ ﯿ نﺎﻣز ﻦ

ﻫد نزُا ﯽ ﻣ و ﯿ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ناﺰ ﯿ

و دراد دﻮﺟو ﻦ 98

زا ﺪﺻرد

اﯾ ﺴﮐﻮﺘﻨ ﯿ تﺪﻣ رد ﺎﻬﻨﺗ ﻦ 1

ﻗد ﯿ ﺰﺠﺗ ﻪﻘ ﯾ دﺮﮔ ﻪ ﯾ ﻀﻌﺑ و ﺪﻧﺪ ﯽ

زا

ﯾ نﻮ ﻈﻧ ﺎﻫ ﯿ ﺴﻠﮐ ،ﻦﻫآ ﺮ ﯿ

ﻣﻮﻟآ ،ﻢ ﯿﻨﯿ ور ،ﻊﻠﻗ ،ﻢ ي

ﺴﻠﮐ تﻼﮐ و ﯿ

ﻢ

اﺰﻓا رد ﯾ ا ﺶ ﯾ ﺘﺒﺜﻣ ﺶﻘﻧ ﺮﺛا ﻦ ﯽ

ﺪﻨﺘﺷاد ] 16 [ ﻘﺤﺗ رد . ﯿ ﺮﺿﺎﺣ ﻖ

ﻧﯿ نﺎﻣز ﺰ ﺎﻫ ي ﻫد نزُا ﯽ ﺮﺿ ﺎﺑ ﯾ ﺒﺗ ﺐ ﯿﯿ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ﺎﺑ ﻻﺎﺑ ﻦ ﯿ

ﻦ

ﮕﺘﺴﺒﻤﻫ ﯽ داد نﺎﺸﻧ ﻨﭽﻤﻫ .

ﯿ ﻟو و ﺎﺟارﺎﮐ ﻦ ﯽ

ﻮﻠﻏوا ) 2007 ( رد

ﺳرﺮﺑ ﯽ ور نزُا ي ﺎﻣ ﺶﻫﺎﮐ ﯾ

ﺴﮐﻮﺗﻮﮑ ﯿ ور ﻦ ي ﻣ ﯿ هﻮ و ﺎﻫ

ﺰﺒﺳ ﯾ ﺎﺠ نزُا ﻪﮐ ،داد نﺎﺸﻧ ت ﯾ

ﮐﺮﺗ ﮏ ﯿ ﺴﮐا ﺐ ﯿ ﺗاﺪ ﯿ ﻠﻋ ﺮﺑ ﻮ ﯿ ا ﻪ ﯾ ﻦ

ﺴﮐﻮﺗ ﯿ ﻦ ﻫ نوﺪﺑ و هدﻮﺑ ﺎﻫ ﯿ

ﺛﺎﺗ عﻮﻧ ﭻ ﯿ

ﻮﺳ ﺮ ﯾ ﯽ ور ي ﻣ ﯿ و هﻮ

ﺰﺒﺳ ﯾ ﺴﮐﻮﺗ تﺎﺠ ﯿ

ﻦ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ﺮﺘﮐﺎﺑ و ﯾﺎﯾ ﯽ ﺑ زا ار ﯿ ﻣ ﻦ ﯽ ﺪﻧﺮﺑ

] 17 [ ﺳرﺮﺑ رد . ﯽ ﮐاﺰﺗرﺰﺗ ﯿ ﭼ و ﺲ ﯿﺮ ﯾ ﺮﮔراﺰ ﯾ ﺲ ) 2008 ( ور ي

ﺛﺎﺗ ﯿ ﻫد نزُا ﺮ ﯽ ﻣ ﺮﺑ ﯿ ﺰﺒﺳ و هﻮ ﯾ ﻠﺣﺮﻣ زا ﺪﻌﺑ تﺎﺠ نزُا ،ﺖﺷادﺮﺑ ﻪ

ﻣﯿ راﺪﻬﮕﻧ ناﺰ ي

ﺪﻨﭼ ار تﻻﻮﺼﺤﻣ ﯾ

اﺰﻓا ﺮﺑاﺮﺑ ﻦ ﯾ

داد ﺶ ] 18 [ .

ﺎﺘﻧ ﯾ ﺳرﺮﺑ ﺞ ﯽ ﺤﺘﻓ ﯽ ﻮﻠﭼﺎﭼآ ﯾ ﯽ نارﺎﮑﻤﻫ و )

1384 ( ور ي ﺛﺎﺗ ﯿ ﺮ

ﻟﻮﺗﺎﭘ راﺪﻘﻣ ﺶﻫﺎﮐ ﺮﺑ لﺎﻌﻓ ﻦﺑﺮﮐ ﯿ

ﺳ بآ رد ﻦ ﯿ

ﻪﮐ داد نﺎﺸﻧ ﺐ

ردﻮﭘ لﺎﻌﻓ ﻦﺑﺮﮐ ي

ﻟﻮﺗﺎﭘ راﺪﻘﻣ ﺶﻫﺎﮐ رد ﻞﮑﺷ ﯿ

ﺳ بآ ﻦ ﯿ ﺐ

ﺧ ﯿﻠ ﯽ ﻟﻮﻧاﺮﮔ عﻮﻧ زا ﺮﺗﺮﺛﻮﻣ ﯽ

ﻤﻋ اﺰﻓا ﺎﺑ و هدﻮﻤﻧ ﻞ ﯾ

ﺖﻈﻠﻏ ﺶ

(10)

ﺐ

اﯾ ﻟﻮﺗﺎﭘ راﺪﻘﻣ ،لﺎﻌﻓ ﻦﺑﺮﮐ عﻮﻧ ﻦ ﯿ

ﻨﻌﻣ رﻮﻃ ﻪﺑ ﻦ ﯽ

راد ي ﺶﻫﺎﮐ

ﻣ ﯽ ﯾ رﻮﻃ ﻪﺑ ،ﺪﺑﺎ ي

فﺮﺼﻣ ﺎﺑ ﻪﮐ 5

ﻟ رد مﺮﮔ ﯿ ﺮﻈﻧ فﺮﺻ نآ زا ﺮﺘ

ﺛﺎﺗ نﺎﻣز زا ﯿ

ﺮ ) 15 ، 5 و 30 ( ﻟﻮﺗﺎﭘ راﺪﻘﻣ ﯿ

ﻣ ﺮﻔﺻ ﻪﺑ ﻦ ﯽ

ﺪﺳر .

ﻨﭽﻤﻫ ﯿ ﺖﻈﻠﻏ رد ﻦ ﺎﻫ

ي اﺰﻓا ﺎﺑ لﺎﻌﻓ ﻦﺑﺮﮐ ﺮﺘﻤﮐ ﯾ

ﺛﺎﺗ نﺎﻣز ﺶ ﯿ

ﺮ

اﺪﻘﻣ ﺶﻫﺎﮐ ﻟﻮﺗﺎﭘ ر

ﯿ ﻨﻌﻣ ﻦ ﯽ دﻮﺑ راد ] 2[

ﻨﭽﻤﻫ . ﯿ زرا رد ﻦ ﯾﺑﺎ ﯽ

ﻣﯿ ﻟﻮﺗﺎﭘ ناﺰ ﯿ ﺳ ﮏﺷاﻮﻟ رد ﻦ ﯿ

ﺳ بآ و ﺐ ﯿ

رد هﺪﺷ ﻪﺿﺮﻋ ﺐ

لﺎﺳ رد دﺮﮐﺮﻬﺷ نﺎﺘﺳﺮﻬﺷ 1394

ﺣر ﻂﺳﻮﺗ ﯿﻤ

ﯽ نارﺎﮑﻤﻫ و

ﻟﻮﺗﺎﭘ ﻪﮐ داد نﺎﺸﻧ ﯿ

رد ﻦ 14 ﺳ ﮏﺷاﻮﻟ ﻪﻧﻮﻤﻧ ﯿ

ﺐ ) 40 ﺪﺻرد ( و

7 ﺳ بآ ﻪﻧﻮﻤﻧ ﯿ

ﺐ ) 20 ﺪﺻرد ( دراد دﻮﺟو ﻣ .

ﯿ ﮕﻧﺎ ﯿ ﺖﻈﻠﻏ ﻦ

ﻟﻮﺗﺎﭘ ﯿ رد ﻦ ﻪﻧﻮﻤﻧ ﺎﻫ ي ﺳ بآ و ﮏﺷاﻮﻟ ﯿ

ﺗﺮﺗ ﻪﺑ ﺐ ﯿ

ﺐ

3/

26 9/

34 و 7/

19 26 ﻣ ﯿ ﮐ رد مﺮﮔوﺮﮑ ﯿ

دﻮﺑ مﺮﮔﻮﻠ ]

19 [

ﻘﺤﺗ رد. ﯿ ﻧ ﺮﺿﺎﺣ ﻖ ﯿ

ﻪﻧﻮﻤﻧ ﺰ ﺎﻫ ي ﺎﻣزآ درﻮﻣ ﯾ

اراد ﺶ ي ﺪﺻرد

ﻟﻮﺗﺎﭘ ﯿ ﻻﺎﺑ ﻦ ﯾ ﯽ ﺪﻧدﻮﺑ ﺳرﺮﺑ رد. ﯽ رﺪﻨﮑﺳا ي ور نارﺎﮑﻤﻫ و ي

ﺛﺎﺗ ﯿ ﻟﻮﺗ ﻒﻠﺘﺨﻣ ﻞﺣاﺮﻣ ﺮ ﯿ

ﺘﻌﻨﺻ ﺪ ﯽ ﻣ ﺮﺑ ﮏﺷاﻮﻟ ﯿ

ﻢﺳ ﺶﻫﺎﮐ ناﺰ

ﻟﻮﺗﺎﭘ ﯿ ﺎﺘﻧ ﻦ ﯾ ﻟﻮﺗﺎﭘ ﺶﻫﺎﮐ ﺮﺜﮐاﺪﺣ ﻪﮐ داد نﺎﺸﻧ ﺞ ﯿ

راﺪﻘﻣ ﻪﺑ ﻦ

60 / 24 و 20 / 18 ﺗﺮﺗ ﻪﺑ ﺪﺻرد ﯿ

ﺳﻻﻮﻣﺮﻓ ﻞﺣاﺮﻣ رد ﺐ ﯿ

و نﻮ

ﻠﻐﺗ ﯿ رد ،ﺖﻓﺮﮔ ترﻮﺻ ﻆ ﻟﺎﺣ

ﯽ ،ندﺮﮐ ﮏﺸﺧ ﻞﺣاﺮﻣ زا ﺪﻌﺑ ﻪﮐ

ﻓﯿ ﺳاﺮﺘﻠ ﯿ ﺗﺎﻣﺪﻘﻣ ﺖﺨﭘ و نﻮ ﯽ

ﻣ ، ﯿ ﻟﻮﺗﺎﭘ ﻢﺳ ﺶﻫﺎﮐ ناﺰ ﯿ

ﻪﺑ ﻦ

ﺗﺮﺗ ﯿ ﺐ 58 /8 ، 82 /3 و 48 /2 دﻮﺑ ﺪﺻرد ﺎﺘﻧ .

ﯾ رد ﻪﮐ داد نﺎﺸﻧ ﺞ

ﺎﻬﻧ لﻮﺼﺤﻣ ﯾ

ﯽ ﺑ ﯿ زا ﺶ 40 ﻟوا راﺪﻘﻣ ﺪﺻرد ﯿ

ﻟﻮﺗﺎﭘ ﻪ ﯿ ﻗﺎﺑ ﻦ ﯽ

ﻣ ﯽ ﺪﻧﺎﻣ ] 20 [ .

ﺳرﺮﺑ ﯽ ﺮﺿﺎﺣ نﺎﺸﻧ داد ﺎﺑ ﻪﺑ ﮔرﺎﮐ ﯿﺮ ي زﺎﮔ نزُا ﻪﺑ ﺮﻤﻫ ها لﺎﻏز

لﺎﻌﻓ ﻣ ﯽ ناﻮﺗ رد تﺎﺠﻧﺎﺧرﺎﮐ ﻟﻮﺗ

ﯿ ﺪ ﻤﺑآ ﯿ هﻮ و هﺮﺘﻧﺎﺴﻨﮐ ﻣ ﯿ ناﺰ

ﻟﻮﺗﺎﭘ ﯿ ﻦ ار رد ﻞﺑﺎﻗﺪﺣ ﻟﻮﺒﻗ ﯽ ﺶﻫﺎﮐ داد و ﺎﺑ شور ﺎﻫ ي ﺐﺳﺎﻨﻣ

زروﺎﺸﮐ ي ) (GAP ﻣﺎﮔ ﯽ ﺮﺛﻮﻣ رد ﺖﻣﻼﺳ ﻪﻌﻣﺎﺟ و تاردﺎﺻ

ﻤﺑآ ﯿ هﻮ و هﺮﺘﻧﺎﺴﻨﮐ ﺖﺷادﺮﺑ .

5 - يراﺰﮕﺳﺎﭙﺳ

ﻦﯾا ﻪﻟﺎﻘﻣ ﻪﺘﻓﺮﮔﺮﺑ زا نﺎﯾﺎﭘ ﻪﻣﺎﻧ هرود ﯽﺳﺎﻨﺷرﺎﮐ ﺎﻨﺻ ﺪـﺷرا

ﯾ ﻊ

اﺬﻏ ﯾ ﯽ بﻮﺼـﻣ هﺎﮕﺸـﻧاد

ﻮﺧ ﺪﺣاو دازا ي

ﯽﻣ ﺪﺷﺎﺑ .

نﺎﮔﺪﻨﺴـﯾﻮﻧ زا

هزﻮـﺣ ﺖـﻧوﺎﻌﻣ هﺎﮕﺸﻧاد ﯽﺸـﻫوﮋﭘ ﺮﮑﺸﺗ

و

ﯽﻧادرﺪﻗ ﺪﻨﯾﺎﻤﻨﯿﻣ .

دراﺪﻧ دﻮﺟو نﺎﮔﺪﻨﺴﯾﻮﻧ ﻦﯿﺑ ﻊﻓﺎﻨﻣ ضرﺎﻌﺗ ﻪﻧﻮﮕﭽﯿﻫ .

6 - ﻊﺑﺎﻨﻣ

[1] Puel, O., Galtier, P., & Oswald, I. P.

(2010). Biosynthesis and toxicological effects of patulin. Toxins, 2(4), 613-631.

[2] FathiAchachloii, B., Ahmadi Zonooz, A., Asadi, y., Hesari, J., Asgari Zakaria, R., (2002). Effect of active carbon on patulin reduction at apple juice. Master Thesis – Tabriz University (Ministry of Science, Research, and Technology). In Persian [3] Poostforoushfard, A., Pishgar, A. R.,

Berizi, E., Nouraei, H., Sobhani, Z., Mirzaie, R., &Zomorodian, K. (2017). Patulin contamination in apple products marketed in Shiraz, Southern Iran. Current Medical Mycology, 3(4), 32.

[4] Tavakkoli, R., Mohammadi, A., Attaran, A., (2011). Determination of the amount of patulin in apple juice using micro-extraction method with dispersed solvent drops with high-performance liquid chromatography.

Master Thesis - Payame Noor University of Markazi Province (Ministry of Science, Research, and Technology). In persin.

[5] Miller, F. A., Silva, C. L., &Brandão, T. R.

(2013). A review on ozone-based treatments for fruit and vegetables preservation. Food Engineering Reviews, 5(2), 77-106

[6] Diao, E., Wang, J., Li, X., Wang, X., Song, H., & Gao, D. (2019). Effects of ozone processing on patulin, phenolic compounds and organic acids in apple juice. Journal of Food science and Technology, 56(2), 957- 965.

[7] Diao, E., Hou, H., Chen, B., Shan, C., &

Dong, H. (2013). Ozonolysis efficiency and safety evaluation of aflatoxin B1 in peanuts. Food and Chemical Toxicology, 55, 519-525.

[8] Ashirifie-Gogofio, J., Floros, J. D.,

&LaBorde, L. F. (2009). Ozone degradation of patulin in model apple juice system. In Annual Meeting & Food Expo, Anaheim, CA: IFT (Vol. 9).

[9] Cataldo, F. (2008). Ozone decomposition of Patulin—a micotoxin and food contaminant. Ozone: Science and Engineering, 30(3), 197-201.

[10] Gökmen, V., Artık, N., Acar, J., Kahraman, N., &Poyrazoğlu, E. (2001).

Effects of various clarification treatments on patulin, phenolic compound and organic acid compositions of apple juice. European Food Research and Technology, 213(3), 194-199.

[11] Ekinci, R. (2013). Effect of activated charcoal on some phenolic compounds of apple juice.Asian Journal of Chemistry, 25 (5): 2905-2908

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[12] Delavar, M., Kamankesh, M., Tavakoli, R., Navabi, A., & Mohammadi, A. 2014.

Determination of patulin in apple juice samples using dispersive liquid-liquid microextraction followed by high performance liquid chromatography and method optimization using response surface methodology. Iranian Journal of Nutrition Sciences & Food Technology, 8 (4): 145- 154.

[13] Kadakal, C., &Nas, S. (2002). Effect of activated charcoal on patulin, fumaric acid and some other properties of apple juice. Food/Nahrung, 46(1), 31-33.

[14] Huebner, H. J., Mayura, K., Pallaroni, L., Ake, C. L., Lemke, S. L., Herrera, P., &

Phillips, T. D. (2000). Development and characterization of a carbon-based composite material for reducing patulin levels in apple juice. Journal of Food Protection, 63(1), 106-110.

[15] EnJie, D., Wei, L., Yue, W., JiaRong, H., Fei, W., ChenLin, W., &XiangYang, L.

(2018). Design and application of ozone detoxification equipment for patulin in contaminated apple juice. Transactions of the Chinese Society of Agricultural

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[16] Karaca, H., &SedatVelioglu, Y. (2009).

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ABSTRACT

2022

May , 19 . Vol , 3 12 . JFST No

Effect of Ozone and Activated Charcoal on Patulin in Apple Concentrate

Bashiri, A. ¹, Matini, S. ^2*, Armideh, Sh.³

1. Master Student, Islamic Azad University, Khoy Branch, Khoy, Iran.

2. Assistant Professor of Food Science Department,Faculty of Agriculture, Islamic Azad University, Khoy Branch, Iran.

3. Associate Professor, Faculty of Agriculture, Urmia University, Iran

ABSTRACT ARTICLE INFO

Article History:

Received 2021/ 11/ 30 Accepted 2022/ 01/ 23 Keywords:

Mycotoxin,Patulin, Ozone,

Activated charcoal, Apple concentrate

Patulin is a major human health problem, especially in beverage industrial and concentrate. The global standard of patulin in fruit juices is about 50 ppb. Studies show that using different physical, chemical and biological methods can reduce the amount of patulin.Therefore, the amount of patulin in apple concentrate samples by ozone, activated charcoal and their combined effects were evaluated. One-way analysis of variance to compare the separate effects of the two factors, two-way analysis of variance to the combined effect and the Tukey test was used to compare the means at the 5% probability level.The results of this study showed that there is a direct relationship between increasing the concentration of activecharcoal and time of ozonationwith decreasing the amount of patulin. The highest decrease in patulin concentration was obtained in 0.2 g of activated charcoal and 9 minutes ozonation. The results also showed that there was an interaction between the two variables and the highest decrease in patulin was observed at 8 minutes of ozonation and 1.5 g of activated charcoal.The present study showed that by using ozone along with activated charcoal, it is possible to reduce the amount of patulin in fruit juice and concentrate production factories and take an effective step in community health and export of fruit juice and concentrate.

DOI: 10.52547/fsct.19.123.189

DOR: 20.1001.1.20088787.1401.19.123.21.6

*Corresponding Author E-Mail:

[email protected]

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