ffl

(1)

1



مﺎﺧ بﻼﺿﺎﻓ زا ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ﻲﻣﻮﺑ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﺖﻴﺻﺎﺼﺘﺧا ﻦﻴﻴﻌﺗ و يزﺎﺳاﺪﺟ

نﺎﮔﺪﻨﺴﻳﻮﻧ :

ﻲﻨﻤﻳا ﻲﻔﻄﺼﻣ ﺪﻴﺳ ﻲﻬﭙﺳ ناﻮﺧا سﺎﺒﻋ ،1

نﺎﻄﻠﺳ يﺪﻬﻣ ﺪﻤﺤﻣ ،2 3لﻻد

1 . ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ ﻪﺘﺧﻮﻣآ ﺶﻧاد

، هوﺮﮔ ﻲﻤﻴﺷ ﻲﺳﺪﻨﻬﻣ -

يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ

، ﻲﻣﻼﺳا دازآ هﺎﮕﺸﻧاد

2 . ﻲﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،يژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ هوﺮﮔ رﺎﻴﺸﻧاد

3 . لﻮﺌﺴﻣ هﺪﻨﺴﻳﻮﻧ ناﺮﻬﺗ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻳاﺬﻏ داﻮﻣ يژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ ،ﻲﻳاﺬﻏ داﻮﻣ ﻲﺳﺎﻨﺷ بوﺮﻜﻴﻣ دﺎﺘﺳا:

سﺎﻤﺗ ﻦﻔﻠﺗ : 982188992971 Email: [email protected] .

ﺖﺷاﺪﻬﺑ عﻮﻠﻃ

ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ ود دﺰﻳ ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ﻢﻫدﺰﻧﺎﭘ لﺎﺳ

هرﺎﻤﺷ : لوا

ﺖﺸﻬﺒﻳدرا و ﻦﻳدروﺮﻓ 1395

ﻞﺴﻠﺴﻣ هرﺎﻤﺷ 55 :

لﻮﺻو ﺦﻳرﺎﺗ :

25 / 5 / 1394

شﺮﻳﺬﭘ ﺦﻳرﺎﺗ :

18 / 6 / 1394

هﺪﻴﻜﭼ

ﻪﻣﺪﻘﻣ سوﺮﻳو ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ: لﻮﻠﺳ ﺮﺑ ﻪﻛ ﺪﻨﺘﺴﻫ ﻲﻳﺎﻫ

و تﻮﻳرﺎﻛوﺮﭘ يﺎﻫ ﻪﺑ

و هﮋﻳ يﺮﺘﻛﺎﺑ ﺐﻳﺮﺨﺗ رد ﺎﻫ

ﺪﻧراﺬﮔﺮﺛا .

نآ ﺮﺛا ﻢﺴﻴﻧﺎﻜﻣ ،ﺖﺳا ﻲﺑﺎﺨﺘﻧا رﺎﻴﺴﺑ ﺎﻫ

ﻪﺑ ﻪﻧﻮﮔ ا ي ﻲﺻﺎﺧ يﺮﺘﻛﺎﺑ ﺮﺑ ﺎﻬﻨﺗ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﺮﻫ ﻪﻛ

ﻲﻣ ﺮﺛا دراﺬﮔ . ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻲﻣ ،هﮋﻳو صاﻮﺧ ﻦﺘﺷاد ﻞﻴﻟد ﻪﺑ ﺎﻫ

ياﺮﺑ ﻲﺒﺳﺎﻨﻣ ﻦﻳﺰﮕﻳﺎﺟ ﺪﻨﻧاﻮﺗ

ﻲﺘﻧآ ﻚﻴﺗﻮﻴﺑ يرﺎﻤﻴﺑ نﺎﻣرد رد ﺎﻫ ﺪﻨﺷﺎﺑ ﻲﻧﻮﻔﻋ يﺎﻫ

. ﺼﺨﻣ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يزﺎﺳاﺪﺟ ﻖﻴﻘﺤﺗ ﻦﻳا فﺪﻫ صﻮ

ﺖﺳا بﻼﺿﺎﻓ زا ﻲﻠﻛﺎﻴﺸﻳﺮﺷا يﺮﺘﻛﺎﺑ .

ﻲﺳرﺮﺑ شور ﻪﻛ بﻼﺿﺎﻓ زا ﻪﻧﻮﻤﻧ ﺖﺸﻫ :

ماﺪﻛﺮﻫ دوﺪﺣ ﻞﻣﺎﺷ 50

ﻣ ﻲﻠﻴ ﺮﺘﻴﻟ و دﻮﺑ مﺎﺧ بﻼﺿﺎﻓ

ﻪﻠﺻﺎﻓﺎﺑ ﻲﻧﺎﻣز 10 ﻪﻴﻔﺼﺗ زا ﻪﻘﻴﻗد ﻊﻤﺟ ناﺮﻬﺗ رد ﻊﻗاو هﺪﻨﮔرز ﻪﻧﺎﺧ

ﺪﺷ يروآ .

ﺖﺸﻛ ﻂﻴﺤﻣ ﺎﺑ ﺲﭙﺳ

Heart Infusion Medium) -

Brain (BHI:

ﻪﺑ ناﻮﻨﻋ ﺖﺸﻛ ﻂﻴﺤﻣ

ﺪﺷر ﺖﻬﺟ ﻊﻳﺎﻣ

ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺪﺷ طﻮﻠﺨﻣ ﺎﻫ

. ﺺﻟﺎﺧ ﻞﻣﺎﺷ رﺎﻛ ﺮﮕﻳد ﻞﺣاﺮﻣ ﺰﻴﻧ نﺎﺑﺰﻴﻣ ﻦﻴﻴﻌﺗ و يﺮﺘﻛﺎﺑ ﺢﻴﻘﻠﺗ ، يزﺎﺳ

ﺪﻧﺪﺷ مﺎﺠﻧا ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يزﺎﺳاﺪﺟ ياﺮﺑ .

ﺖﻬﺟ ﻲﺑﺎﺨﺘﻧا ندﻮﺑ ﺛﺄﺗ ﺮﻴ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يور ﺮﺑ

ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺎﻫ

ﺪﻨﻧﺎﻣ ﻲﻳ E.coli (ATCC25922): Enterococcus faecalis (ATCC19433)،

،

Staphylococcus aureus (ATCC2392) Yersinia enterocolitica (ATCC9610)و

ﺪﺷ ﻲﺳرﺮﺑ .

ﻪﺘﻓﺎﻳ ﺎﻫ ﺪﺷ اﺪﺟ ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ﺐﺨﺘﻨﻣ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﻧﻮﻤﻧ ﺖﺸﻫ ﺮﻫ زا: .

ﻪﻛ داد نﺎﺸﻧ نﺎﺑﺰﻴﻣ ﻦﻴﻴﻌﺗ ﺶﻳﺎﻣزآ

،ﺎﻫژﺎﻓ ﻦﻳا ﻪﺑ

ﺑﻮﺧ ﻲ ﺘﺴﻫ ﻲﻠﻛﺎﻴﺸﻳﺮﺷا يﺮﺘﻛﺎﺑ ندﺮﺑ ﻦﻴﺑ زا و ندﺮﻛ ﺰﻴﻟ ﻪﺑ ردﺎﻗ ﺮﻳﺎﺳ يور ﺎﻣا،ﺪﻨ

يﺮﺘﻛﺎﺑ يﺎﻫ هدﺎﻔﺘﺳادرﻮﻣ ﭻﻴﻫ ،

ﺛﺄﺗ يﺮﻴ ﺪﻧراﺪﻧ .

ﻪﺠﻴﺘﻧ يﺮﻴﮔ ﺞﻳﺎﺘﻧ: ﻪﺑ ﺖﺳد هﺪﻣآ ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﻛ داد نﺎﺸﻧ ﻪﺑ

ترﻮﺻ ﻲﻣ ﻞﻤﻋ ﻲﺻﺎﺼﺘﺧا ﺪﻨﻨﻛ

. ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ

ا ﻪﻜﻨﻳ ﻲﺘﻧآ ﻪﺑ ﺖﻣوﺎﻘﻣ ناﺰﻴﻣ ﻚﻴﺗﻮﻴﺑ

ﻲﻣ ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ،ﺖﺳا ﺶﻳاﺰﻓا لﺎﺣ رد ﺎﻫ ياﺮﺑ ﻲﺒﺳﺎﻨﻣ ﻦﻳﺰﮕﻳﺎﺟ ﺪﻨﻧاﻮﺗ

ﻲﺘﻧآ دﺮﺑرﺎﻛ ﻚﻴﺗﻮﻴﺑ

ﺖﻧﻮﻔﻋ نﺎﻣرد رد ﺎﻫ ﺪﻨﺷﺎﺑ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ يﺎﻫ

.

هژاو يﺪﻴﻠﻛ يﺎﻫ ﻲﺘﻧآ ،ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ،بﻼﺿﺎﻓ ،ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ:

ﻚﻴﺗﻮﻴﺑ

(2)

ﻪﻣﺪﻘﻣ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺖﻣوﺎﻘﻣ هزوﺮﻣا ﻲﺘﻧآ ﺮﺑاﺮﺑ رد ﺎﻫ

ﻚﻴﺗﻮﻴﺑ ﻪﺑ ﺎﻫ

ناﺮﮕﻧ و ﻢﻬﻣ ﻲﻋﻮﺿﻮﻣ ﻲﻧﺎﻬﺟ نﺎﻣرد و ﺖﺷاﺪﻬﺑ رد هﺪﻨﻨﻛ

ﺪﺒﺗ ﻞﻳ هﺪﺷ ﺖﺳا . ﻦﻳازا ور شﻼﺗ ﺐﺳﺎﻨﻣ ﻲﻨﻳﺰﮕﻳﺎﺟ ﻦﺘﻓﺎﻳ ياﺮﺑ ﺎﻫ

ﻲﺘﻧآ ياﺮﺑ ﻚﻴﺗﻮﻴﺑ

نآ تاﺮﻀﻣ ﻪﻛ ﺎﻫ ﻪﺑ

صﻮﺼﺧ رد ﺖﻣوﺎﻘﻣ دﺎﺠﻳا

ﻲﻣ يروﺮﺿ ﺮﻣا ﻚﻳ ﺪﺷﺎﺑ ﻪﺘﺷاﺪﻧ ار نﺎﻣرد ﺮﺑاﺮﺑ ﺪﺷﺎﺑ

. زا ﻲﻜﻳ

ﻲﻣ ﻪﻛ يدراﻮﻣ نﺎﻣرد رد ﺪﻧاﻮﺗ

،دﻮﺷ هدﺎﻔﺘﺳا ﻲﻳﺎﻳﺮﺘﻛﺎﺑ يﺎﻫ

ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﺪﻨﺘﺴﻫ ﺎﻫ

. ژﺎﻓ ﺎﻳ ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ سوﺮﻳو ﺎﻫ

ﺮﺑ ﻪﻛ ﺪﻨﺘﺴﻫ ﻲﻳﺎﻫ

لﻮﻠﺳ ﻲﻣ ﺮﺛا تﻮﻳرﺎﻛوﺮﭘ يﺎﻫ ﺪﻧراﺬﮔ

. هﺪﻧرﻮﺧ ﻲﻨﻌﻣ ﻪﺑ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ

ﺑ ﻲﻣ يﺮﺘﻛﺎ ﻦﻳا و ﺪﺷﺎﺑ

مﺎﻧ يراﺬﮔ ياﺪﺘﺑا رد ﻪﻛ ﺖﺳا نآ ﻞﻴﻟد ﻪﺑ

نآ ﻒﺸﻛ ﻲﻣ نﺎﻤﮔ ﺎﻫ

نآ ﻪﻛ ﺪﺷ يﺮﺘﻛﺎﺑ ﺎﻫ

ﻲﻣ ار ﺎﻫ ﺪﻧرﻮﺧ ) 1،2 .(

ﻪﻧﻮﮔ ﺮﻳﺎﺳ ﺪﻨﻧﺎﻤﻫ سوﺮﻳو يﺎﻫ

ﻢﺴﻴﻧﺎﮔرا ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﺎﻫ ﺪﻨﺘﺴﻫ ﻲﻳﺎﻫ

ﻖﻳﺮﻃ زا و ﺪﻧراد نﺎﺑﺰﻴﻣ لﻮﻠﺳ ﻚﻳ ﻪﺑ زﺎﻴﻧ نﺪﻧﺎﻣ هﺪﻧز ياﺮﺑ ﻪﻛ ﻗ لﻮﻠﺳ نآ زا ﻪﻳﺬﻐﺗ ﻲﻣ ﺮﻴﺜﻜﺗ و ءﺎﻘﺑ ﻪﺑ ردﺎ

ژﺎﻓ توﺎﻔﺗ ،ﺪﻨﺷﺎﺑ رد ﺎﻫ

ﺖﻴﺻﺎﺧ ﺑﺎﺨﺘﻧا ﻲ ندﻮﺑ نآ ﻲﻣ ﺎﻫ ﺪﺷﺎﺑ ﻪﺑ ﻪﻧﻮﮔ ا ي ﺮﺑ ﺎﻬﻨﺗ ژﺎﻓ ﺮﻫ ﻪﻛ

ﺮﺛا ﻲﺻﺎﺧ يﺮﺘﻛﺎﺑ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺮﻳﺎﺳ ﺮﺑ و راﺬﮔ

ﺎﻫ ﺑ ﻲ ﺮﻴﺛﺄﺗ

ﻲﻣ ﺪﺷﺎﺑ ) 2،3 .(

ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ لﻮﻠﺳ رد و ﺖﻌﻴﺒﻃ ﺮﺳاﺮﺳ رد ﺎﻫ

يﺎﻫ

ﻲﻳﺎﻳﺮﺘﻛﺎﺑ نﺎﺑﺰﻴﻣ ﻪﺑ

ترﻮﺻ ﺪﻨﺘﺴﻫ هﺪﻨﻛاﺮﭘ هدﺮﺘﺴﮔ )

4،5 .(

رد

ﻪﻫد ﻲﺘﻧآ زا دﺎﻳز هدﺎﻔﺘﺳا ﺮﻴﺧا يﺎﻫ ﻚﻴﺗﻮﻴﺑ

هﺪﻳﺪﭘ ﺐﺟﻮﻣ نﺎﻣرد رد ﺎﻫ

ﻲﺘﻧآ ﺖﻣوﺎﻘﻣ ياﺮﺑ يﺪﺟ ﺮﻄﺧ ﻚﻳ هﻮﻘﻟﺎﺑ ﻦﻳا ﻪﻛ ﺖﺳا هﺪﺷ ﻲﻜﻴﺗﻮﻴﺑ

ﻲﻣ ﻲﻧﺎﻬﺟ ﺖﺷاﺪﻬﺑ هﺪﻨﻳآ ﺪﺷﺎﺑ

. ﻦﻳازا ور ﺐﺟﻮﻣ ﻪﻛ ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ

ﻲﻤﻧ ﺖﻣوﺎﻘﻣ ﺘﻛﺎﺑ ﻦﺘﻓر ﻦﻴﺑ زا ﺐﺒﺳ ﺰﻴﻧ دﻮﺧ و ﺪﻧﻮﺷ

و يﺮ ﺷﻼﺘﻣ ﻲ

نﺪﺷ ﻲﻣ نآ لﺎﺳ رد ،ﺪﻧﻮﺷ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻞﻣاﻮﻋ نﺎﻣرد ﺮﻴﺧا يﺎﻫ

هدﺮﻛ ﺐﻠﺟ دﻮﺧ ﻪﺑ ار يدﺎﻳز تﺎﻬﺟﻮﺗ ﺪﻧا

) 6 . ( ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ

نآ ﺮﺘﺸﻴﺑ و ﺪﻨﺘﺴﻫ توﺎﻔﺘﻣ ﻞﻜﺷ رد ﺎﻫ ﻲﻟﻮﻃ ياراد ﺎﻫ

ﻪﺑ هزاﺪﻧا 200 - 24 ﻮﻧﺎﻧ ﺪﻧراد ﺮﺘﻣ . ياراد ژﺎﻓ ﺮﻫ ﺮﺴﻜﻳ

ﺪﻴﺴﭙﻛ ﻚﻳ و

لﻮﻠﺳ ﻲﻜﻴﺘﻧژ تﺎﻋﻼﻃا ﻪﻛ ﺖﺳا ﻲﻣ ﺰﻴﻧ و دراد راﺮﻗ نآ رد

رد ﺪﻨﻧاﻮﺗ

ﺪﻨﺷﺎﺑ توﺎﻔﺘﻣ ﻞﻜﺷ و هزاﺪﻧا )

9 - 7 .(

،نﺎﺑﺰﻴﻣ لﻮﻠﺳ ﻪﺑ دورو ياﺮﺑ

دﻮﺧ ﺪﻴﺳا ﻚﻴﺗﻮﺌﻠﻛﻮﻧ ﻖﻳرﺰﺗ ﺎﺑ و هﺪﻴﺒﺴﭼ لﻮﻠﺳ ﺢﻄﺳ ﻪﺑ ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﺑ ردﺎﻗ نآ ﻲﻜﻴﺘﻧژ تﺎﻋﻼﻃا زا هدﺎﻔﺘﺳا و لﻮﻠﺳ ﻞﺧاد ﻪﺑ ﻟﻮﺗ

ﻞﺜﻣﺪﻴ

ﻲﻣ ﺪﺷﺎﺑ ) 10 .(

يﺮﺘﻛﺎﺑ زا ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ﻲﻳﺎﻫ

ﻪﻛ ﺖﺳا ﻪﺑ رﻮﻃ رد ﻲﻌﻴﺒﻃ

تﻻﺎﺣ ﺪﻨﻧﺎﻣ ﻲﺻﺎﺧ ﻂﻳاﺮﺷ رد ﻲﻟو دراد رﻮﻀﺣ نﺎﺴﻧا هدور رﻮﻠﻓ ﻏ يدﺎﻋﺮﻴ ﻂﻳاﺮﺷ ﺎﻳ و نﺎﺑﺰﻴﻣ لﻮﻠﺳ ﻲﻨﻤﻳا ﻢﺘﺴﻴﺳ ﻒﻌﺿ ،هدور

ﻲﻣ ﻲﻄﻴﺤﻣ ﺪﻋﺎﺴﻣﺎﻧ يرﺎﻤﻴﺑ ﺐﺟﻮﻣ ﺪﻧاﻮﺗ

ﻲﻳﺎﻫ ﻪﻠﻤﺟزا ،ﺲﻴﺴﭙﺳ

ﺖﻧﻮﻔﻋ دﺎﺠﻳا يدازﻮﻧ ﺖﻳﮋﻨﻨﻣ و ﺖﻳﺮﺘﻧاوﺮﺘﺳﺎﮔ ،ﻢﺧز يﺎﻫ

ﺪﻨﻛ ) 11،12 .(

ﻦﻳا فﺪﻫ ﻲﻣﻮﺑ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يزﺎﺳاﺪﺟ ﺮﺑ شﻼﺗ ﻪﻌﻟﺎﻄﻣ

ﻲﻣ مﺎﺧ بﻼﺿﺎﻓ زا ﻲﻠﻛﺎﻴﺸﻳﺮﺷا يﺮﺘﻛﺎﺑ ﺪﺷﺎﺑ

.

ﻲﺳرﺮﺑ شور

ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يزﺎﺳاﺪﺟ :

بﻼﺿﺎﻓ زا ﻪﻧﻮﻤﻧ ﺖﺸﻫ ماﺪﻛﺮﻫ

دوﺪﺣ ﻞﻣﺎﺷ

50 ﻣ ﻲﻠﻴ ﺮﺘﻴﻟ ﺰﻴﻧ و مﺎﺧ بﻼﺿﺎﻓ ﻪﻠﺻﺎﻓﺎﺑ

ﻲﻧﺎﻣز 10 زا ﻪﻘﻴﻗد

ﻪﻴﻔﺼﺗ ﻊﻤﺟ ناﺮﻬﺗ رد ﻊﻗاو هﺪﻨﮔرز ﻪﻧﺎﺧ ﺪﺷ يروآ

. ﺲﭙﺳ ﻤﻧ ﻪﻧﻮ ﺎﻫ زا

هرﺎﻤﺷ 8 - 1 هرﺎﻤﺷ ﻚﻳ تﺪﻣ و هﺪﺷ يراﺬﮔ ﻪﻧﺎﺒﺷ

زور لﺎﭽﺨﻳ رد

ﻪﺑ رﻮﻈﻨﻣ ﻪﺗ نآ ﻪﻴﻟوا ﻲﮔدﻮﻟآ ﻲﻨﻴﺸﻧ ﺪﻧﺪﺷ يراﺪﻬﮕﻧ ﺎﻫ

. ﺲﭙﺳ

ﻪﻧﻮﻤﻧ رود ﺎﺑ ماﺪﻛﺮﻫ ﺎﻫ 4000

ﻊﻳﺎﻣ و هﺪﺷ ژﻮﻔﻳﺮﺘﻧﺎﺳ ﻪﻘﻴﻗد رد رود

ﻪﻟﻮﻟ رد نآ ﻲﻳور ﻊﻤﺟ ﻪﻧﺎﮔاﺪﺟ يﺎﻫ

ﺪﺷ يروآ .

80 ﻣ ﻲﻠﻴ ﺮﺘﻴﻟ زا

(Brain, Heart Broth) BH Broth

ﺲﭙﺳ و ﻪﻴﻬﺗ ﺮﻳﺎﻣ ﻦﻟرا رد

10 ﻲﻠﻴﻣ ﺮﺘﻠﻴﻓ زا رﻮﺒﻋ زا ﺲﭘ ﻪﻧﻮﻤﻧ ﺮﻫ زا ﺮﺘﻴﻟ 22

/ 0 ﺎﺑ نوﺮﻜﻴﻣ 10

ﻲﻠﻴﻣ ثاﺮﺑ لﻮﻠﺤﻣ زا ﺮﺘﻴﻟ ﻬﺗ

ﻪﻴ هﺪﺷ ﺪﻧﺪﺷ ﺐﻴﻛﺮﺗ .

ﺎﺑ ﺮﺘﻠﻴﻓ زا رﻮﺒﻋ

(3)

3

ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣود

دﺰﻳ



دﺎﻌﺑا هﺪﺷﺮﻛذ ﻲﻣ ﺐﺒﺳ لﻮﻠﺤﻣ يوﺎﺣ ﻪﻟﻮﻟ دراو يﺮﺘﻛﺎﺑ ﭻﻴﻫ ﺎﺗ دﻮﺷ

دﻮﺸﻧ ثاﺮﺑ .

ازاﺪﻌﺑ ﻦﻳ ﻞﺣاﺮﻣ 10 ﻲﻠﻴﻣ هزﺎﺗ ﺖﺸﻛ لﻮﻠﺤﻣ زا ﺮﺘﻴﻟ

ﻲﻠﻛﺎﻴﺸﻳﺮﺷا يﺮﺘﻛﺎﺑ ATCC 25922 )

( ﻚﻳ ﺎﻬﻨﺗ ﻪﻛ ﻪﻧﺎﺒﺷ

زور زا

ﻲﻣ نآ ﺖﺸﻛ ﻲﻣ ﻪﻓﺎﺿا ﻪﻟﻮﻟ ﺮﻫ ﻪﺑ ،درﺬﮔ

دﻮﺷ . ﻪﻧﻮﻤﻧ ﺎﻫ ﻪﺑ رﻮﻈﻨﻣ

ﺮﺘﻬﺑ طﻼﺘﺧا ﻪﺑ

ﺑﻮﺧ ﻲ ﻪﺑ ﺳو ﻪﻠﻴ هﺎﮕﺘﺳد Vortex ﻲﻣ هدز ﻢﻫ ﻪﺑ

ﺪﻧﻮﺷ .

ﻪﻧﻮﻤﻧ مﺎﻤﺗ نﺎﻳﺎﭘ رد تﺪﻣ ﻪﺑ ﺎﻫ

24 ﺖﻋﺎﺳ يﺎﻣد ﺎﺑ رﻮﺗﺎﺑﻮﻜﻧا رد 37

ﻲﺘﻧﺎﺳ ﻪﺟرد ﻲﻣ راﺮﻗ داﺮﮔ

ﺪﻧﺮﻴﮔ .

ﺺﻟﺎﺧ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يزﺎﺳ :

زا ﺪﻌﺑ 24 ﻪﻧﻮﻤﻧ ﺖﻋﺎﺳ ناﺰﻴﻣ و جرﺎﺧ ﺎﻫ

ﻲﻣ ﻲﺳرﺮﺑ ﻪﻟﻮﻟ ﺮﻫ تروﺪﻛ ﻪﻧﻮﻤﻧ ﻪﻛ دﻮﺷ

تروﺪﻛ ياراد ﺎﻫ

ﻲﺒﻳﺮﻘﺗ 1 ﺪﻧدﻮﺑ ﺪﻨﻟرﺎﻓ ﻚﻣ ﻲﻟا .

هرﺎﻤﺷ ﻪﻟﻮﻟ 3

يﺮﺘﻤﻛ تروﺪﻛ ﻪﻛ

،دﻮﺑ اراد ار ﻪﺑ

ناﻮﻨﻋ ﻪﻧﻮﻤﻧ ﻛ يا ،ﺖﺳا نآ رد ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻦﻳﺮﺘﺸﻴﺑ ﻪ

ﺢﻴﺿﻮﺗ ﻞﺣاﺮﻣ ﻪﻴﻠﻛ و ﺪﻳدﺮﮔ بﺎﺨﺘﻧا هداد

هﺪﺷ ﻪﻧﻮﻤﻧ ﻦﻳا ياﺮﺑ 3

ﺎﺗ ﺪﻳدﺮﮔ راﺮﻜﺗ ﻪﺒﺗﺮﻣ

ًادوﺪﺣ تروﺪﻛ ﻪﺑ 5

/ 0 ﺪﺳﺮﺑ ﺪﻨﻟرﺎﻓ ﻚﻣ .

ﻪﻧﻮﻤﻧ

ًادﺪﺠﻣ ﻪﻟﻮﻟ ﻚﻳ ﻞﺧاد ﻪﺑ ﺖﻧﺎﺗﺎﻧﺮﭘﻮﺳ و هﺪﺷ ژﻮﻔﻳﺮﺘﻧﺎﺳ

ﺪﺷ ﻞﻘﺘﻨﻣ ،ﻞﻳﺮﺘﺳا .

رد ﺺﻟﺎﺧ زﺎﺳ ي نآ ﻪﺑ ﻪﻛ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻜﺷ

ﻞ ﻫد ﻲ

ﺎﻳ كﻼﭘ ﺪﺣاو Plaque Forming Units

ﻪﻛ ﻪﺑ رﺎﺼﺘﺧا ) (PFU

شور زا ﺪﻨﻳﻮﮕﻴﻣ Double-Layer Agar

(DLA) ﺎﻳ رد ﻪﻛ

ﻪﻌﻟﺎﻄﻣ Sambrook و

Russel ) 13،14 ( نآ ﻪﺑ ﻪﺘﺧادﺮﭘ هﺪﺷ ،ﺖﺳا

ﺪﻳدﺮﮔ هدﺎﻔﺘﺳا .

ﻪﺑ رﻮﻃ ،ﻪﺻﻼﺧ 100 ﻲﻠﻴﻣ ژﺎﻓ ﻪﻧﻮﻤﻧ لﻮﻠﺤﻣ زا ﺮﺘﻴﻟ ﺎﺑ

100 ﻣ ﻲﻠﻴ ﺮﺘﻴﻟ ﺖﺸﻛ هزﺎﺗ يﺮﺘﻛﺎﺑ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ زا هداد

هﺪﺷ ﻪﻛ 24

يﺎﻣد رد ﺖﻋﺎﺳ 37

ﺲﭙﺳ و هﺪﺷ طﻮﻠﺨﻣ ،هدﻮﺑ داﺮﮔ ﻲﺘﻧﺎﺳ ﻪﺟرد

ﻪﺑ 4 ﻣ ﻲﻠﻴ ﺮﺘﻴﻟ رﺎﮔآ زا 7 / 0 % ﺪﻳدﺮﮔ ﻪﻓﺎﺿا .

ﻪﻛ دﻮﺷ ﺖﻗد ﺪﻳﺎﺑ

ﺐﺳﺎﻨﻣ طﻼﺘﺧا نآ

ﺎﻫ ﺖﺳا ﺖﻴﻤﻫا ﺰﺋﺎﺣ .

ﺖﻴﻠﭘ يور طﻮﻠﺨﻣ ﺲﭙﺳ

90 ﻲﻠﻴﻣ يوﺎﺣ ﻪﻛ يﺮﺘﻣ 10

ﻲﻠﻴﻣ ﺮﺘﻴﻟ رﺎﮔآ 5 / 1 % ا ﺪﺷ ﻪﺘﺨﻳر ،ﺖﺳ زا ﺪﻌﺑ و

10 ﺎﻫ ﺖﻴﻠﭘ ﻪﻛ ﻪﻘﻴﻗد

ًﻼﻣﺎﻛ ،ﺪﻧﺪﺷ ﻚﺸﺧ قﺎﺗا يﺎﻣد رد

تﺪﻣ ﻪﺑ 24 يﺎﻣد ﺎﺑ رﻮﺗﺎﺑﻮﻜﻧا رد ﺖﻋﺎﺳ 37

ﻲﺘﻧﺎﺳ ﻪﺟرد داﺮﮔ

ﺪﻨﺘﻓﺮﮔ راﺮﻗ .

ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ نﺎﺑﺰﻴﻣ لﻮﻠﺳ ﻦﻴﻴﻌﺗ :

يﺮﺘﻛﺎﺑ ﻦﻴﻴﻌﺗ فﺪﻫ يﺪﻌﺑ مﺎﮔ رد

ﻪﻛ ﺖﺳا يژﺎﻓ نﺎﺑﺰﻴﻣ ﻪﺑ

ﺖﺳد هﺪﻣآ ﺖﺳا . ﻪﺑ رﻮﻈﻨﻣ ﻲﺳرﺮﺑ ﺮﻴﺛﺄﺗ ژﺎﻓ

جاﺮﺨﺘﺳا هﺪﺷ ﻦﻴﻨﭽﻤﻫ و ﻪﺑ

رﻮﻈﻨﻣ

ندﺮﻛ ﻞﻤﻋ ﻲﺻﺎﺼﺘﺧا ﺶﻳﺎﻣزآ

ﺮﻳﺎﺳ ﻪﺑ ژﺎﻓ لﻮﻠﺤﻣ ،نآ ﺮﺘﻛﺎﺑ

ي ﺎﻫ ﺪﻳدﺮﮔ ﻪﻓﺎﺿا .

رﻮﻈﻨﻣ ﻦﻳا ياﺮﺑ

لﻮﻠﺤﻣ رد هزﺎﺗ ﺖﺸﻛ ﺪﻨﭼ BHI Broth

زﺎﻓ رد ﻲﮕﻤﻫ ﻪﻛ

يﺮﺘﻛﺎﺑ زا ،ﺪﻧدﻮﺑ ﺪﺷر ﻲﻨﺤﻨﻣ ﻲﻤﺘﻳرﺎﮕﻟ E.coli(ATCC يﺎﻫ

25922)

، Yersinia enterocolitica (ATCC 9610) ،

Staphylococcus aureus (ATCC 2392) Enterococcus و

faecalis(ATCC 19433)

ﻪﻴﻬﺗ هدﺮﻛ ﺲﭙﺳ ماﺪﻛﺮﻫ زا

لﻮﺤﻣ رد و ﺪﻧﺪﺷ ﻪﺘﺨﻳر زورﺎﮔآ يوﺎﺣ ﺖﻴﻠﭘ رد ار ﻲﻳﺎﻳﺮﺘﻛﺎﺑ يﺎﻫ

ﺮﺧآ مﺎﮔ 1

ﻲﻠﻴﻣ ﺮﺘﻴﻟ ﻦﻳا زا ﻚﻳ ﺮﻫ ﻪﺑ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يوﺎﺣ لﻮﻠﺤﻣ زا

ﺪﻳدﺮﮔ ﻪﻓﺎﺿا ﺎﻫ ﺖﻴﻠﭘ .

تﺪﻣ ﻪﺑ ﺲﭙﺳ 24

يﺎﻣرد رد ﺖﻋﺎﺳ 37

ﻪﺟرد ﺘﻧﺎﺳ ﻲ داﺮﮔ ﺪﻧﺪﺷ هداد راﺮﻗ .

ﻪﺘﻓﺎﻳ ﺎﻫ ﺺﻟﺎﺧ زا ﺪﻌﺑ ار نآ ،ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يزﺎﺳ

ور ﺮﺑ ي يوﺎﺣ ﺖﻴﻠﭘ

يﺎﻣد رد نﻮﻴﺳﺎﺑﻮﻜﻧا زا ﺲﭘ و هدﻮﻤﻧ ﻪﻓﺎﺿا ﻲﻠﻛﺎﻴﺸﻳﺮﺷا 37

ﻪﺟرد

ﻲﺘﻧﺎﺳ تﺪﻣ ﻪﺑ داﺮﮔ 24

ﺮﺑ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﻛ ﺪﺷ هﺪﻫﺎﺸﻣ ، ﺖﻋﺎﺳ

ار نآ و ﻪﺘﺷاﺬﮔ ﺮﺛا يﺮﺘﻛﺎﺑ ﻪﺑ

ﺑﻮﺧ ﻲ ﺐﺒﺳ و هدﺮﺑ ﻦﻴﺑ زا و هدﺮﻛ ﺰﻴﻟ

ﺖﺳا هﺪﺷ رﺎﮔآ ﺢﻄﺳ رد ﻲﻟﺎﺧ طﺎﻘﻧ دﺎﺠﻳا )

ﻞﻜﺷ 1 .(

ﻲﻣ ﻞﻤﻋ ﺮﻳﺬﭘ بﺎﺨﺘﻧا رﺎﻴﺴﺑ ﺎﻫژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﺪﻨﻨﻛ

: يﺮﺘﻛﺎﺑ توﺎﻔﺘﻣ يﺎﻫ

،ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ عﻮﻧ ﻚﻳ ﺎﺑ درﻮﺧﺮﺑ رد ﺬﭘ بﺎﺨﺘﻧا

يﺮﻳ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ يﻻﺎﺑ

(4)

Y

Staphylococ Yersinia

ﻲﺑ و هﺪﻧﺎﻣ ﺮﻴﻴﻐﺗ

نﺎﺸﻧ ﻲ ﻦﻳا هﺪﻨﻫد ﻪﺘﻓر ﻦﻴﺑ زا و هﺪﺷ

ﺎ ﻢﺴﻴﻧﺎﻜﻣ ﺰﻴﻧ و ﺖﻣوﺎﻘﻣ ﻪﺑ ﻪﻛ

ﺖ ) 15،16 .(

دﺎﺠﻳا ﺐﺟﻮﻣ نآ ﻲﻣﻮﻤﻋ ﺖﻣﻼﺳ ت

ت هرود زا ﺲﭘ ﺎﻫ

ﻪﻛ ﺪﻨﺘﻓﺮﮔ ﻪﺠﻴﺘﻧ لﺮﺘﻨﻛ ،فاﺮﻃا ﻂ

دﻮﻤ ) . 19 .(

رد بﻼﺿﺎﻓ رد د ﺪﻧراد هرﺎﺷا .

ﻦﻳا

ﻦﻴﺑ زا ار ﺎﻣ نﻮ

ﺰﻴﻧ Abdulla و

ﺎﻫ ژﺎﻓﻮ ﻪﺑ ناﻮﻨﻋ

يﺮﺘﻛ ccus يﺎﻫ

Enteroc و

ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ژﺎﻓﻮ ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ﻪﺑ ط

ﺮﺘﻛﺎﺑ ﻦﻳا ﺷ ﺰﻴﻟ ي

رد ﺎﻫ ﺑ يرﺎﻤﻴ ﺎﻫ

ﻲﺘﻧآ ﺮ ﻚﻴﺗﻮﻴﺑ ﺎﻫ

ﺖﺳا ﺶﻳاﺰﻓا ﻪﺑ ور ﻪﻛ ﻚ ﺘﻧرد ﻪﺠﻴ آ

تﻼﻜﺸﻣ زا هراﻮﻤ ﻲﻣ ﻲﻜ تﺪﻣ ﺪﻧاﻮﺗ

) 17،18 .(

ﻧ ﺪﻧداد مﺎﺠﻧا ﻪﻛ ﻂ ﺖﺴﻳز ﻂﻴﺤﻣ و ﻮﻤﻧ هدﺎﻔﺘﺳا بﻼﺿ

دﻮﺟﻮﻣ ژﺎﻓﻮﻳﺮﺘﻛ لﺎﻌﻓ ﻦﺠﻟ ﻪﺳوﺮﭘ

ي ﻴﭘ ﺮﻀﻣ يﺎﻫ ﻮﻣاﺮ

ﺪﻨﺷ ) 22 - 20 .(

و ﺶﻧارﺎﻜﻤﻫ وB ﻲ ﻮﻳﺮﺘﻛﺎﺑ زا ناﻮﺗ

ﻲﻣ هﺪ ﻛﺎﺑ ﻪﻛ دﻮﺷ

occus faec ﻮﻳﺮﺘﻛﺎﺑ ﺮﺑاﺮﺑ رد طﻮﺑﺮﻣ ﺖﻴﻠﭘ ﻪﻛ ﺖ

ﻞﺑ ﻪﻈﺣﻼﻣ يا ا زا

يﺮﻴﮔ يﺮﺘﻛﺎﺑ ﺶﻘﻧ در ﺮﺛا ندﻮﻤﻧ دوﺪﺤ

نﺎﻬﺟ رد ،ﺖﺳا ﻲﺘﻧآ فﺮﺼﻣ ﻚﻴﺗﻮﻴﺑ

ﻲﻣ ﻲﻜﻴ ﻤﻫ ،دﻮﺷ

ﻲﺘﻧآ ﺖﻣوﺎﻘ ﻜﻴﺗﻮﻴﺑ

دﻮﺷ ﺖﻓﺎﻳ ﻂﻴﺤﻣ ﻪﻌﻟﺎﻄﻣ ﺶﻧارﺎ يا

ژﺎﻓﻮ رد ﺎﻫ ﺤﻣ ﻂﻴ

ﺿﺎﻓ و بآ ﻪﻴﻔﺼﺗ ﺶﻘﻧ ﻪﺑ ﺰ ﺮﺛﺆﻣ

ﺎﺑ

بﻼﺿ ﻪﺑ و هﮋﻳ ﭘ رد

ﻪ ﮔدﺎﺳ ﻲ يﺮﺘﻛﺎﺑ

ﺎﺑ ﻪﺘﺷاد يرﺮﺿ د ﻪﻛ ﺖ Borrego ﻲﻣ ﻪﻛ ﺪﻧدﺮﻛ ه

ﻞﻜﺷ رد 2 ﺪﻫﺎﺸﻣ

aureus alis ،

enterocolitica ﺖﺳا ﻲﻟﺎﺣ رد ﻦﻳ

راﺪﻘﻣ ﻪﻛ ﺖﺳ ﺎﻗ

ﺖﺳ . ﻪﺠﻴﺘﻧ و ﺚﺤ ﮔ

ﻲﻧاﺮﮕ رﻮﻣ رد ﺎﻫ

نآ ﻞﻤﻋ ﺤﻣ رد ﺎﻫ

رﻮﻬﺸﻣ ﻲﻳﺎﻳﺮﺘﻛﺎ ﻣ راﺪﻘﻣ و ﺖﻴﻓﺮﻇ ﻲﺘﻧآ ﺖﻣوﺎﻘﻣ ﻴﺗﻮﻴﺑ

ﺖﺳا نﺎﻬﺟ رد .

ﻘﻣ

ﻣ رد نآ زا هدﺎﻔﺘﺳ Raghu ﺎﻜﻤﻫ و

ﻲﻣ ﻮﻳﺮﺘﻛﺎﺑ زا ناﻮﺗ

ﻢﻠﻴﻓﻮﻴ رد ﺰﻴﻧ و ﺎﻫ ﺰﻴﻧ ﻲﻤﻛ تﺎﻌﻟﺎﻄﻣ

ﺿﺎﻓ و بآ ﻪﻴﻔﺼﺗ ژﺎﻓ ﻲﻣ ﺎﻫ ﺪﻨﻧاﻮﺗ ﻪﺑ

ﺪﻧﺮﺒ ﺑ ﻲ ﻪﻜﻧآ دﻮﺧ

ﺖﺳا ﺖﻠﻋ ﻦﻴﻤﻫ ﻪ هرﺎﺷا ﺶﻧارﺎﻜﻤﻫ

ﺑﻮﺧ ﻲ ﺮﺑاﺮﺑ ر د

s a ﻳا ﺳا ﺳا ﺤﺑ

ﮕﻧ ﻋ ﺎﺑ ﻇ ﻣ د ﺳا

ﻣ ﻴﺑ ﻣ ﺼﺗ ﻓ ﺒﺑ ﻪﺑ ﻫ

ﻠﻛﺎﻴﺸﻳﺮﺷ ار ﻲ ﻪﺑ ﺧ

رد ﻪﻛ ﺖﺳا ﻲﻟﺎﺣ ) ﻞﻜﺷ 2 .(

ﻲﻠﻛ ﺎﻴﺸﻳﺮﺷا يو

يﺎﻫ ﻪﻌﻟﺎﻄﻣدرﻮﻣ

ﺷا ،ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﻛ ﺣ رد ﻦﻳا و ﻪﺘﺷاﺬ

ً

ﻲﺑ ﺮﺛا ﺖﺳا هدﻮﺑ

وﺎﺣ ﺖﻴﻠﭘ يور ﺮﺑ ژ

يﺮﺘﻛﺎﺑ يور ﺮﺑ ژ ﻫ

ﺪﻧداد ﻪﺑ ﻪﻧﻮﮔ ا ي ﻛ

ﺮﻴﺛﺄﺗ ﺬﮔ ﻲﺑﻮﻠﻄﻣ

يﺮﺘﻛﺎﺑ يﺎ

ًﺎﺒﻳﺮﻘﺗ

1 : ﺮﻴﺛﺄﺗ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ

ﻞ 2 : ﺮﻴﺛﺄﺗ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ

د نﺎﺸﻧ ار ﺎﻫ و هدﺮﻛ ﺰﻴﻟ ﻪﻧﻮﮔ ﺮﻳﺎﺳ ﺎﻫ

ﻞﻜﺷ

(5)

5

دﺰﻳ



هﺪﻨﻨﻛدﻮﺑﺎﻧ يﺎﻫ

يﺮﺘﻛﺎﺑ ﻲﻜﻳژﻮﻟﻮﻴﺑ بﺎﺴﭘ ﻪﻴﻔﺼﺗ رد نژﻮﺗﺎﭘ يﺎﻫ

دﺮﻛ هدﺎﻔﺘﺳا )

23،24 .(

Zumstein و

ﻪﻌﻟﺎﻄﻣ ﺶﻧارﺎﻜﻤﻫ يﺮﺘﻛﺎﺑ ﺖﻴﻌﻤﺟ يور ﺮﺑ ار يا

و ﺎﻫ

ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻲﺑ ﻪﻴﻔﺼﺗ رد ﺎﻫ

ﺪﻧدﺮﻛ مﻼﻋا و ﺪﻧداد مﺎﺠﻧا بﺎﺴﭘ يزاﻮﻫ

ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﻛ ﻪﺳوﺮﭘ ﻦﻳا رد ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺖﻴﻌﻤﺟ ﺮﺑ ذﻮﻔﻧ ﺮﺑ ﺪﻧردﺎﻗ ﺎﻫ

ﺛﺄﺗ راﺬﮔﺮﻴ ﺪﻨﺷﺎﺑ ) 25 .(

ﻪﻣادا رد Periasamy

Sundaram و ﻪﻛ ﺪﻧداد شراﺰﮔ

ﻮﻳﺮﺘﻛﺎﺑ يﺮﺘﻛﺎﺑ ندﺮﺑ ﻦﻴﺑ زا ﻪﺑ ردﺎﻗ ﺎﻫ ژﺎﻓ ﺮﻴﻈﻧ نژﻮﺗﺎﭘ يﺎﻫ

ﺪﻨﺘﺴﻫ ﻲﻧﺎﺘﺳرﺎﻤﻴﺑ بﺎﺴﭘ رد ار ﻲﻠﻛﺎﻴﺸﻳﺮﺷا )

26 .(

ﺎﺑ ﺐﻟﺎﻄﻣ ﻪﺑ ﻪﺟﻮﺗ ناﻮﻨﻋ

هﺪﺷ ﻲﻣ ﺮﻈﻧ ﻪﺑ ﺮﻄﺧ ﻦﺘﻓﺮﮔ ﺮﻈﻧ رد ﺎﺑ ﺪﺳر

ﺖﻣوﺎﻘﻣ گرﺰﺑ ﺘﻧآ

ﻲ ﻲﻜﻴﺗﻮﻴﺑ ﻪﻛ ﺪﻴﺳر ﺪﻫاﻮﺧ يزور ﻪﻛ ﻪﺘﻜﻧ ﻦﻳا و

ﻲﺘﻧآ زا يرﺎﻴﺴﺑ ﻚﻴﺗﻮﻴﺑ

يرﺎﻤﻴﺑ نﺎﻣرد ﻪﺑ ردﺎﻗ ﺮﮕﻳد ﺎﻫ و ﺪﻨﺘﺴﻴﻧ ﺎﻫ

ﻦﺘﻓﺎﻳ نآ ﺮﺑ هوﻼﻋ ﺘﻧآ

ﻲ ﻚﻴﺗﻮﻴﺑ يﺎﻫ و دﻮﺑ ﺪﻫاﻮﺧ راﻮﺷد ﺰﻴﻧ ﺪﻳﺪﺟ

ﻲﺳرﺮﺑ ﺎﺑ ﻦﻴﻨﭽﻤﻫ ﺛﺄﺗ

تاﺮﻴ ﻲﺘﻧآ ﻲﺒﻧﺎﺟ ﻚﻴﺗﻮﻴﺑ

ﺖﺳا رﻮﺒﺠﻣ ﺮﺸﺑ ،ﺎﻫ

يرﺎﻤﻴﺑ نﺎﻣرد ياﺮﺑ هزﺎﺗ ﻲﻫار ﻳﺮﺘﻛﺎﺑ يﺎﻫ

ﻪﻌﺟﺎﻓ زا يﺮﻴﮔﻮﻠﺟ و ﻲﻳﺎ

ﺪﻨﻛ اﺪﻴﭘ ،هﺪﻨﻳآ رد ﻲﻧﺎﺴﻧا .

ارد ﻦﻳ ﻦﻴﺑ ﻪﻛ ﺎﻫ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ، ﻪﻧﻮﮔ

ا ي زا

و سوﺮﻳ ﺎﻫ ﺪﻧردﺎﻗ ﻪﻛ ﺪﻨﺘﺴﻫ ﺑ

ﻲ ﭻﻴﻫ بﺎﺨﺘﻧا ﺖﻴﺻﺎﺧ ﺎﺑ و يرﺮﺿ

يﺮﺘﻛﺎﺑ ،ﻻﺎﺑ يﺮﻳﺬﭘ ﻲﻣ ،ﺪﻨﻨﻛ دﻮﺑﺎﻧ ار دﻮﺧ فﺪﻫ يﺎﻫ

ﺪﻨﻧاﻮﺗ

ﺪﻨﺷﺎﺑ ﻲﺒﺳﺎﻨﻣ ﻦﻳﺰﮕﻳﺎﺟ .

ﻲﺘﺣ ﺎﻫ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻪﭽﻧﺎﻨﭼ

نﺪﺑ ﻞﺧاد نﺎﺴﻧا

داﻮﻣ دﻮﺒﻧ ﺮﺛا رد دﻮﺧ ﺎﻳ فﺪﻫ يﺮﺘﻛﺎﺑ ندﺮﻛ موﺪﻌﻣ زا ﺲﭘ ﺪﻧﻮﺷ ﻲﻣ ﻦﻴﺑ زا ،ﻲﺑﺎﺨﺘﻧا ﺖﻴﺻﺎﺧ ﻞﻴﻟد ﻪﺑ ﻲﻳاﺬﻏ ﻊﻓد نﺪﺑ زا ﺎﻳ و ﺪﻧور

ﻲﻣ ﺪﻧﻮﺷ . تﺎﻌﻟﺎﻄﻣ ﻦﻴﻨﭽﻤﻫ مﺎﺠﻧا

هﺪﺷ ﻲﻣ نﺎﺸﻧ ژﺎﻓ ﻪﻛ ﺪﻫد ﺎﻫ

ﻲﻣ ﺪﻨﻧاﻮﺗ ﻪﺑ ﺑﻮﺧ ﻲ ﺎﺑ ﻪﺑ ﮔرﺎﻛ يﺮﻴ ﻪﻴﻔﺼﺗ رد بﺎﺴﭘ

ﺎﻫ بﺎﺴﭘ ﻲﺘﺣ و يﺎﻫ

ﺑ ،ﻲﻧﺎﺘﺳرﺎﻤﻴﺑ ﻚﻤﻛ ﻪ

ﺤﻣ ﻂﻴ ﺖﺴﻳز ﺪﻨﻳﺎﻴﺑ .

ﺖﻴﻤﻫا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ يﺎﻫ

هﺪﺷﺮﻛذ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ درﻮﻣ رد

ﻦﻳا رد ،ﺎﻫ

بﻼﺿﺎﻓ زا ﻲﻠﻛﺎﻴﺸﻳﺮﺷا يﺮﺘﻛﺎﺑ ﻪﺑ طﻮﺑﺮﻣ ﻲﻣﻮﺑ ژﺎﻓﻮﻳﺮﺘﻛﺎﺑ ﻖﻴﻘﺤﺗ يﺮﺘﻛﺎﺑ ندﺮﺑ ﻦﻴﺑ زا رد نآ ﻲﻳﺎﻧاﻮﺗ و يزﺎﺳاﺪﺟ مﺎﺧ يﺮﻬﺷ ﺮﺑﺎﻨﺑ ﺪﺷ هﺪﻫﺎﺸﻣ ﻲﻠﻛﺎﻴﺸﻳﺮﺷا ﻲﻣ ﻦﻳا

ناواﺮﻓ ﻊﺒﻨﻣ ﻦﻳا زا ناﻮﺗ

ﺘﻛﺎﺑ بﻼﺿﺎﻓ ﻪﻴﻔﺼﺗ رد ﺰﻴﻧ و ﻲﻧﺎﻣرد و ﻲﺘﺷاﺪﻬﺑ ﻊﻳﺎﻨﺻ رد ژﺎﻓﻮﻳﺮ

يﺮﻬﺷ هﺮﻬﺑ دﺮﺑ .

ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ

ﻪﻟﺎﻘﻣ ﻦﻳا

تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ بﻮﺼﻣ ﻲﺗﺎﻘﻴﻘﺤﺗ حﺮﻃ ﻞﺻﺎﺣ

تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻳاﺬﻏ داﻮﻣ يژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ دادراﺮﻗ هرﺎﻤﺷ ﻪﺑ ناﺮﻬﺗ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ 31123

ﺪﺷﺎﺑ ﻲﻣ . ﻦﻴﻨﭽﻤﻫ

زا ﻪﻟﺎﻘﻣ نﺎﮔﺪﻨﺴﻳﻮﻧ دﺎﻫﺮﻓ ﺮﺘﻛد

اﺮﻫز ﻢﻧﺎﺧ رﺎﻛﺮﺳ ،ﻲﻫاﻮﺨﻜﻴﻧ

تﺎﻤﺣز و ﺎﻫ ﺖﻳﺎﻤﺣ ﻞﻴﻟد ﻪﺑ ﻲﻧﺎﺠﻧﺎﺧ ﺪﻴﻌﺳ سﺪﻨﻬﻣ ﺰﻴﻧ و ﻲﺒﺟر ﺪﻧراد ار ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ لﺎﻤﻛ نﺎﺸﻧاواﺮﻓ .

References

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The use of antibiotics to improve phage detection and enumeration by the double layer agar technique, journal of BMC Microbiology.2009; 9:148

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Etiology of uro pathogens and antimicrobial resistance of common uro pathogens. Med Sci Moni.

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9

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Isolating E.Coli Bacteriophage from Raw Sewage and Determining its Selectivity to the Host Cell

Imeni SM (M.Sc)¹, Akhavan Sepahi A (Ph.D)², Soltan Dallal MM (Ph.D)³

1. M.Sc, Department of Chemical engineering, Islamic Azad University, Tehran, Iran 2. Associate Professor, Islamic Azad University, Tehran, Iran

3. Corresponding Author: Professor of Food Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Bacteriophages are viruses that infect and destroy prokaryote cells, specifically the bacteria. They act too selective, so as each bacteriophage affects only on specific type of bacteria. Due to their specific features, bacteriophages can be used as an appropriate substitute for antibiotics in infectious diseases treatment. Therefore, this study aimed to isolate E. coli-specific bacteriophage from raw sewage.

Methods: Eight samples of raw sewage, each containing approximately 50 ml of raw sewage with 10 minute gap, were prepared from Zargandeh wastewater treatment plant, Tehran, Iran. The sewages were mixed with Brain-heart infusion medium (BHI) as a liquid culture medium in order to let the microorganisms grow. Incubation, purification and determination of bacteria were followed repeatedly to isolate the bacteriophage. Then it was tested on E.coli (ATCC 25922), Enterococcus faecalis (ATCC 19433), Staphylococcus aureus (ATCC 2392), and Yersinia enterocolitica (ATCC 9610) in order to determine the bacteriophage selectivity.

Results: The E.coli bacteriophages were successfully isolated from all the eight samples, that were completely able to lyse and destroy E.coli bacterial cells, though no effect was observed on other types of bacteria.

Conclusion: The study findings revealed that bacteriophages act selectively. Considering the raise of antibiotic resistance in the world, bacteriophages can serve as a good substitute for antibiotics in treating infectious diseases.

Keywords: Antibiotics, Bacteriophage,E.coli, Sewage