ORIGINAL ARTICLE
Received:2016/03/12 Accepted:2016/05/30
The Effect of Electron Irradiation on Aflatoxin B 1 in Pistachio Production Process Inoculated
with Aspergillus Flavus
Bahador HajiMohammadi (Ph.D)
1, Mohammad Hassan Ehrampoush (Ph.D)
2, Sima Hashemi (MS.c)
3, Sepideh Khalatbari Limaki (MS.c)
4,
Fatemeh Zare (MS.c)
5,Moslem taheri soudejani (MS.c)
6
1.Assistant Professor Department Food Hygiene and Safety, Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2.Professor, Department of Environmental Health Engineering, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3.Corresponding Author: MSc Student, Department of Food Safety and Hygiene, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Email: [email protected] Tel: 09172557224
4.MS.c of Food Safety and Hygiene, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5.MS.c of Department Immunology, Reprodactive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
6.MS.c of Epidemiology, Department of Biostatistics and Epidemiology, School of Public Health, Yazd University of Medical Sciences,Yazd, Iran
Abstract
Introduction: Pistachio is an important export product in some countries, especially Iran. Pistachios are with the highest risk of aflatoxin contamination between agricultural products. Aflatoxin B1 is the most potent carcinogenic aflatoxins. Aflatoxins have an important role in the etiology of cancer. Undoubtedly, the major problem is, Aspergillus flavus contamination. Aflatoxin production and find suitable ways to prevent its production. The aim of this study was to determine the effect of electron irradiation on aflatoxin B1
production in pistachio that was inoculated with Aspergillus flavus.
Methods: Experimental study was performed. aflatoxigenic Aspergillus flavus spores (106 spores/ml) were inoculated into 10 pistachio samples. Contaminated samples were exposed to the electron beam radiations at doses of 1, 3, 5 and 7 kGy. The samples were stored at a room temperature for 0, 30, 60, 90, 180, 210 days and were compared with the control samples for the aflatoxin production by Kruskal Wallis test using SPSS version 16. Aflatoxin B1 was measured by ELISA kit. The test was duplicated for each samples.
Results: The mean concentration of aflatoxin in the samples was 59.97 ng/g. There was no difference among the production of aflatoxin in different doses on days 0,30, 60, 90 (p<0/05), but on days 180 and 210 was borderline significant (p=0/06).
Conclusion: Exposure of the pistachio samples by electron beam could inhibit the growth and alsoprevent aflatoxin production.
Keywords: Aspergillus flavus, Aflatoxins B1, Electron beam, Pistachio Conflict of interest: The authors declared that there is no Conflict interests.
This Paper Should be Cited as:
The Effect of Electron Irradiation on Aflatoxin B1 in Pistachio Production Process Inoculated with… J Tolooebehdasht Sci 2017; 16(2):1-8. [Persian]
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
ﺪﻴﻟﻮﺗ ﺪﻧورﺮﺑ ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘﺮﺛا ﻲﺳرﺮﺑ آ
ﻦﻴﺴﻛﻮﺗﻼﻓ
B
1سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ ﺎﺑ هﺪﺷ ﺢﻴﻘﻠﺗ يﺎﻫ ﻪﺘﺴﭘ رد
يﺪﻤﺤﻣ ﻲﺟﺎﺣ ردﺎﻬﺑ :نﺎﮔﺪﻨﺴﻳﻮﻧ شﻮﭘ ماﺮﺣا ﻦﺴﺣﺪﻤﺤﻣ ،
1،
2ﻲﻤﺷﺎﻫ ﺎﻤﻴﺳ ﻲﻛﺎﻤﻴﻟ يﺮﺒﺘﻌﻠﺧ هﺪﻴﭙﺳ ،
3عراز ﻪﻤﻃﺎﻓ ،
4ﻢﻠﺴﻣ ،
5ﻲﻧﺎﺟدﻮﺳ يﺮﻫﺎﻃ
6
1 . ،اﺬﻏ ﻲﻨﻤﻳا و ﺖﻣﻼﺳ تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ رﺎﻳدﺎﺘﺳا هﺪﻜﺸﻧاد
،ﺖﺷاﺪﻬﺑ دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
2 دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ،ﻂﻴﺤﻣ ﺖﺷاﺪﻬﺑ ﻲﺳﺪﻨﻬﻣ هوﺮﮔ دﺎﺘﺳا.
3 يﻮﺠﺸﻧاد :لﻮﺌﺴﻣ هﺪﻨﺴﻳﻮﻧ . هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ،ﻲﻳاﺬﻏداﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ هوﺮﮔ ،ﻲﻳاﺬﻏ داﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ
:سﺎﻤﺗ ﻦﻔﻠﺗ دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ 09172557224
Email: [email protected]
4 ﺷرﺎﻛ . هﺪﻜﺸﻧاد ،ﻲﻳاﺬﻏداﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ هوﺮﮔ ،ﻲﻳاﺬﻏ داﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ هوﺮﮔ ،ﻲﻳاﺬﻏ داﻮﻣ ﻲﻨﻤﻳا و ﺖﺷاﺪﻬﺑ ﺪﺷرا ﻲﺳﺎﻨ
دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ
5 اد ،يژﻮﻟﻮﻧﻮﻤﻳا هوﺮﮔ ،ﻞﺜﻣ ﺪﻴﻟﻮﺗ ﻲﺳﺎﻨﺷ ﻲﻨﻤﻳا تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ ،يژﻮﻟﻮﻧﻮﻤﻳا ﺪﺷرا سﺎﻨﺷرﺎﻛ . ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧ
دﺰﻳ ﻲﻗوﺪﺻ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد
6 ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد ،يژﻮﻟﻮﻴﻣﺪﻴﭘا و رﺎﻣآ هوﺮﮔ ،يژﻮﻟﻮﻴﻣﺪﻴﭘا ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ .
دﺰﻳ ﻲﻗوﺪﺻ
ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ ود دﺰﻳ ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد لﺎﺳ ﻢﻫدﺰﻧﺎﺷ
:هرﺎﻤﺷ مود
ﺮﻴﺗ و دادﺮﺧ 1396
:ﻞﺴﻠﺴﻣ هرﺎﻤﺷ 62
:لﻮﺻو ﺦﻳرﺎﺗ 22
/ 10 / 1394
:شﺮﻳﺬﭘ ﺦﻳرﺎﺗ 10
/ 3 / 1395 هﺪﻴﻜﭼ
:ﻪﻣﺪﻘﻣ ﻲﺧﺮﺑ رد ﻲﺗاردﺎﺻ ﻢﻬﻣ لﻮﺼﺤﻣ ﻚﻳ ﻪﺘﺴﭘ ﻪﺑ ﺎﻫرﻮﺸﻛ
هﮋﻳو ﺖﺳا ناﺮﻳا
تﻻﻮﺼﺤﻣ ﻦﻴﺑ رد و
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻪﺑ ﻲﮔدﻮﻟآ ﺮﻄﺧ ﻦﻳﺮﺗﻻﺎﺑ ﺎﺑ يزروﺎﺸﻛ ﻦﻴﺴﻛﻮﺗﻼﻓآ .دراد راﺮﻗ
B1
نﺎﻃﺮﺳ ﻞﻴﺴﻧﺎﺘﭘ ﻦﻳﺮﺘﺸﻴﺑ ار ﻲﻳاز
سﻮﻠﻳژﺮﭙﺳآ ﻪﺑ ﻪﺘﺴﭘ ﻲﮔدﻮﻟآ ﻪﻠﺌﺴﻣ ،ﻪﺘﺴﭘ تاردﺎﺻ ﻪﺻﺮﻋ رد رﻮﺸﻛ ﻲﻠﺻا ﻞﻀﻌﻣ .دراد ﺎﻫ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻦﻴﺑ رد ﻴﺴﻛﻮﺗﻼﻓآ و سووﻼﻓ ﻦﻴﻴﻌﺗ ﻪﻌﻟﺎﻄﻣ ﻦﻳا زا فﺪﻫ .ﺖﺳا نآ ﺪﻴﻟﻮﺗ زا يﺮﻴﮕﺸﻴﭘ ﺖﻬﺟ ﺐﺳﺎﻨﻣ هار ندﺮﻛ اﺪﻴﭘ و ﻦ
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ ﺪﻧور ﺮﺑ ﻲﻧوﺮﺘﻜﻟا ﻮﺗﺮﭘ ﺮﺛا B1
.دﻮﺑ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ ﺎﺑ هﺪﺷ ﺢﻴﻘﻠﺗ يﺎﻫ ﻪﺘﺴﭘ رد
:ﻲﺳرﺮﺑ شور .ﺪﺷ مﺎﺠﻧا (ﻲﺑﺮﺠﺗ)يا ﻪﻠﺧاﺪﻣ ترﻮﺻ ﻪﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳا
ﺪﻴﻟﻮﺗ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ رﻮﭙﺳا هﺪﻨﻨﻛ
ﻪﺑ ﻦﻴﺴﻛﻮﺗﻼﻓآ 10
ﻪﻧﻮﻤﻧ .ﺪﻳدﺮﮔ ﺢﻴﻘﻠﺗ ،ﻪﺘﺴﭘ هﺪﺷ يﺪﻨﺑ ﻪﺘﺴﺑ ﻪﻧﻮﻤﻧ ﻪﺘﺴﭘ يﺎﻫ
رد يﺎﻫزود 1
، 3
، 5 و 7
يﺮﮔﻮﻠﻴﻛ .ﺪﻨﺘﻓﺎﻳ ﻪﻬﺟاﻮﻣ ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ ﺎﺑ
ﺎﻫ ﻪﻧﻮﻤﻧ ﺲﭙﺳ رد
ﻲﻧﺎﻣز هزﺎﺑ
،ﺮﻔﺻ 210،180،90،60،30 رد زور
يﺎﻣد
ﻂﻴﺤﻣ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ ﺮﻈﻧ زا ﺪﻫﺎﺷ يﺎﻫ ﻪﻧﻮﻤﻧ ﺎﺑ و هﺪﻳدﺮﮔ يراﺪﻬﮕﻧ B1
راﺰﻓا مﺮﻧ ﺎﺑ ﻪﺨﺴﻧSPSS
16
نﻮﻣزآ ﻂﺳﻮﺗ Kruskal Wallis
ﺪﻧﺪﺷ ﻪﺴﻳﺎﻘﻣ .
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺶﺠﻨﺳ B1
راﺮﻜﺗ رﺎﺑ ود ﺎﺑ و اﺰﻳﻻا ﺖﻴﻛ ﻂﺳﻮﺗ
.ﺪﻳدﺮﮔ مﺎﺠﻧا
:ﺎﻫ ﻪﺘﻓﺎﻳ ﺎﻫ ﻪﻧﻮﻤﻧ ﻞﻛ رد ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺖﻈﻠﻏ ﻦﻴﮕﻧﺎﻴﻣ
97 / 59 ﺪﻴﻟﻮﺗ ناﺰﻴﻣ ﻦﻴﺑ .دﻮﺑ مﺮﮔ ﺮﺑ مﺮﮔﻮﻧﺎﻧ
،ﺮﻔﺻ يﺎﻫزور رد ﻒﻠﺘﺨﻣ يﺎﻫزود رد ﻦﻴﺴﻛﻮﺗﻼﻓآ 90،60،30
هﺪﻫﺎﺸﻣ يرﺎﻣآ ﺮﻈﻧ زا يراد ﻲﻨﻌﻣ ﺮﻴﻴﻐﺗ
)ﺪﻳدﺮﮕﻧ 05 /
>0 يﺎﻫزور رد (P 180
و 210 )دﻮﺑ يراد ﻲﻨﻌﻣ ﺢﻄﺳ ﻪﺑ ﻚﻳدﺰﻧ 06
P=/
.(
:يﺮﻴﮔ ﻪﺠﻴﺘﻧ راﺮﻗ
ﻢﺳ ﺪﻴﻟﻮﺗ زا چرﺎﻗ ندﺮﺑ ﻦﻴﺑ زا ﺎﺑ ﺪﻧاﻮﺗ ﻲﻣ ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ ضﺮﻌﻣ رد ﻪﺘﺴﭘ يﺎﻫ ﻪﻧﻮﻤﻧ نداد
.ﺪﻳﺎﻤﻧ يﺮﻴﮕﺸﻴﭘ ﻦﻴﺴﻛﻮﺗﻼﻓآ
هژاو يﺎﻫ :يﺪﻴﻠﻛ سﻮﻠﻳژﺮﭙﺳآ
،سووﻼﻓ ﻦﻴﺴﻛﻮﺗﻼﻓآ B1
، ﻮﺗﺮﭘ
،ﻲﻧوﺮﺘﻜﻟا ﻪﺘﺴﭘ
ﻦﻳا
،ﻪﻟﺎﻘﻣ ﻞﺻﺎﺣ نﺎﻳﺎﭘ ﻪﻣﺎﻧ يﻮﺠﺸﻧاد ﻲﺳﺎﻨﺷرﺎﻛ ﺪﺷرا هوﺮﮔ ﺖﺷاﺪﻬﺑ و ﻲﻨﻤﻳا داﻮﻣ ﻲﻳاﺬﻏ هﺪﻜﺸﻧاد ﺖﺷاﺪﻬﺑ هﺎﮕﺸﻧاد
مﻮﻠﻋ ﻲﻜﺷﺰﭘ و تﺎﻣﺪﺧ ﻲﺘﺷاﺪﻬﺑ ﻲﻧﺎﻣرد ﺪﻴﻬﺷ ﻲﻗوﺪﺻ ﻲﻣدﺰﻳ .ﺪﺷﺎﺑ
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
) ﻪﺘﺴﭘ Pistacia vera L.
هداﻮﻧﺎﺧ ﻪﺑ ﻖﻠﻌﺘﻣ ( Anacardiaceeae
ﺖﺳا ﻲﺑﺮﻏ يﺎﻴﺳآ و ﺮﻴﻐﺻ يﺎﻴﺳآ ﻲﻣﻮﺑ ﻪﻛ هدﻮﺑ )
1 .( لﻮﺼﺤﻣ
ﻪﺑ ﻪﺘﺴﭘ تاﺪﻴﻟﻮﺗ رد ﻲﺻﺎﺧ ﺖﻴﻤﻫا ﻲﺗاردﺎﺻ لﻮﺼﺤﻣ ﻚﻳ ناﻮﻨﻋ
ﻪﺑ ﺎﻫرﻮﺸﻛ ﻲﻀﻌﺑ رد يزروﺎﺸﻛ هﮋﻳو
دراد ناﺮﻳا )
2 (.
ناﺮﻳا ًﺎﺘﻌﻴﺒﻃ
ﺎﻣا ﺖﺳا ﻲﻧﺎﻬﺟ ﻪﺘﺴﭘ ﺪﻴﻟﻮﺗ ﺖﺴﺨﻧ ﻪﺒﺗر رد ﻪﺑ
ندﻮﺑ ﻻﺎﺑ ﻞﻴﻟد
ﺮﻈﻧ زا ﻲﺑﻮﺧ ﺖﻴﻌﻗﻮﻣ لﻮﺼﺤﻣ ﻦﻳا ،ﻪﺘﺴﭘ رد ﻦﻴﺴﻛﻮﺗﻼﻓآ ناﺰﻴﻣ ﻪﺘﺴﭘ تاردﺎﺻ رد ﺶﻟﺎﭼ ﺚﻋﺎﺑ ﻦﻳا و دراﺪﻧ ﻲﻧﺎﻬﺟ ﻲﺑﺎﻳرازﺎﺑ ﺖﺳا هﺪﺷ ناﺮﻳا )
2 (.
ﻪﺘﺴﭘ ﺎﺑ يزروﺎﺸﻛ تﻻﻮﺼﺤﻣ ﻦﻴﺑ رد
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻲﮔدﻮﻟآ ﻪﺑ ﺮﻄﺧ ﻦﻳﺮﺗﻻﺎﺑ ﺪﺷﺎﺑ ﻲﻣ
) 3 ( و ﻪﺘﺴﭘ هﻮﻴﻣ .
ﻮﻈﻨﻣ ﻪﺑ ﻪﺘﺴﭘ يوﺎﺣ تﻻﻮﺼﺤﻣ ﺪﻨﻧﺎﻣ ﻲﺑوﺮﻜﻴﻣ ﺖﻴﻔﻴﻛ ﻲﺑﺎﻳزرا ر
راﺮﻗ ﺶﻳﺎﻣزآ درﻮﻣ ﻦﻴﺴﻛﻮﺗﻼﻓآ حﻮﻄﺳ و ﻲﭼرﺎﻗ ﻲﮔدﻮﻟآ ﻲﻣ ﺪﻧﺮﻴﮔ ) 4 (.
ﺎﻫ ﻦﻴﺴﻛﻮﺗﻮﻜﻳﺎﻣ ﺖﻴﻟﻮﺑﺎﺘﻣ
يﺎﻫ ﻪﻳﻮﻧﺎﺛ ﺪﻨﺘﺴﻫ چرﺎﻗ ﻪﻛ
تاﺮﻴﺛﺄﺗ
ﻲﺑﺮﺨﻣ ﺮﺑ يور تﺎﻧاﻮﻴﺣ و نﺎﺴﻧا ﺪﻧراد
يرﺎﻤﻴﺑ ﻪﺑ ﺮﺠﻨﻣ ﻪﻛ ﺎﻫ
و
نﺎﻳز يﺎﻫ يدﺎﺼﺘﻗا ﻲﻣ
دﻮﺷ . ﻲﮔدﻮﻟآ داﻮﻣ
ﻲﻳاﺬﻏ و ﻪﻳﺬﻐﺗ ﺎﺑ
ﻦﻴﺴﻛﻮﺗﻮﻜﻳﺎﻣ رد ﺎﻫ
ﺮﺳاﺮﺳ ﻚﻳ نﺎﻬﺟ ﻞﻜﺸﻣ
ﻢﻬﻣ ﺖﺳا ) 5 (.
ﻪﻛ ﺖﺳا ﻲﻫﺎﻴﮔ ﻒﻴﻌﺿ نژﻮﺗﺎﭘ ﻚﻳ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ ﻪﺑ ﻲﻣ هﺪﻳد ﻢﻟﺎﺳ ﻪﺘﺴﭘ ﺰﻐﻣ رد ترﺪﻧ .دﻮﺷ
هداد نﺎﺸﻧ تﺎﻌﻟﺎﻄﻣ ﺪﻧا
ﻪﺘﺴﭘ ﻲﻧاﺪﻨﺧ دوز ﻪﻛ ﻲﻣ ﺎﻫ
يﺎﻫ چرﺎﻗ رﻮﭙﺳا ﺎﺑ ار ﻪﺘﺴﭘ ﺰﻐﻣ ﺪﻧاﻮﺗ
ﺎﺑ ﺢﻄﺳ ﺎﺑ ﻦﻴﺴﻛﻮﺗﻼﻓآ هﺪﻨﻨﻛ ﺪﻴﻟﻮﺗ ،ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻲﮔدﻮﻟآ يﻻ
)ﺪﻨﻛ ﻪﺟاﻮﻣ 5،6
( ﺎﻫ ﻦﻴﺴﻛﻮﺗﻮﻜﻳﺎﻣ ﻦﻳﺮﺘﻤﻬﻣ زا ﺎﻫ ﻦﻴﺴﻛﻮﺗﻼﻓآ .
و سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ ﻲﭼرﺎﻗ ﻪﻧﻮﮔ ود ﻂﺳﻮﺗ ًﺎﺗﺪﻤﻋ ﻪﻛ ﺪﻨﺘﺴﻫ ﻲﻣ ﺪﻴﻟﻮﺗ سﻮﻜﻴﺘﻳزارﺎﭘ سﻮﻠﻳژﺮﭙﺳآ نآ ﻲﻠﺻا عﻮﻧ رﺎﻬﭼ .دﻮﺷ
ﺎﻫ
AFB1
AFB2 ، AFG1 ،
AFG2 و ﻲﻣ ﺪﻨﺷﺎﺑ ) 10 - 6
، 3 ( .
هداد نﺎﺸﻧ دﺪﻌﺘﻣ تﺎﻌﻟﺎﻄﻣ ﻲﻣ ﺎﻫ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻪﻛ
رد ﺪﻨﻧاﻮﺗ ﺪﻧﻮﺷ ﺪﻴﻟﻮﺗ يرادرﺎﺒﻧا ﺎﻳ و ﺪﻨﻳآﺮﻓ ﻲﻃ ،ﻪﻋرﺰﻣ
) 11 (.
ﻦﻴﺴﻛﻮﺗﻼﻓآ
نﺎﻃﺮﺳ ﻲﻤﺳ ﺶﻬﺟ ،از
از ﻒﻴﻌﻀﺗ و نﺪﺑ ﻲﻨﻤﻳا ﻢﺘﺴﻴﺳ هﺪﻨﻨﻛ
ﺪﺷﺎﺑ ﻲﻣ ) 2 (.
ﻪﻧﻮﮔ رﻮﻀﺣ ﻪﺑ ﺪﻳﺎﺑ ﻪﺘﺴﭘ رد سﻮﻠﻳژﺮﭙﺳآ يﺎﻫ
ياﺮﺑ يﺪﺟرﻮﻃ
ﻲﻳﺎﻫرﻮﺘﻛﺎﻓ .دﻮﺷ ﺶﻳﺎﭘ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ و ﻲﻧاﺪﻨﺧ ﻞﻴﺒﻗ زا
ﺖﺑﻮﻃر 88 - 78 ﻪﻧاﻮﺟ ياﺮﺑ ﺪﺻرد و سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ ﻲﻧز
ﺖﺳا زﺎﻴﻧدرﻮﻣ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ )
10 (.
ﻪﺑ هراﻮﻤﻫ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ چرﺎﻗ لواﺪﺘﻣ ناﻮﻨﻋ
چرﺎﻗ ﻦﻳﺮﺗ
هدﻮﻟآ ﻦﻳا .ﺖﺳا هﺪﺷﺮﻛذ ﻲﻤﻠﻋ ﻊﺑﺎﻨﻣ رد ﻲﻳاﺬﻏ داﻮﻣ هﺪﻨﻨﻛ ﻪﻧﻮﮔ
هﮋﻳو ﻞﻳﺎﻤﺗ ﻪﻧاد ﻲﮔدﻮﻟآ ياﺮﺑ يا
ﻠﻴﺟآ يﺎﻫ تﻼﻏ و ﻲﻨﻏور و ﻲ
ﻲﻣ نﺎﺸﻧ دﻮﺧ زا و ﻲﺑﺮﭼ ندﻮﺑ اراد ﺎﺑ تﻻﻮﺼﺤﻣ ﻦﻳا .ﺪﻫد
و ﻲﮔدﻮﻟآ ياﺮﺑ تﻻﻮﺼﺤﻣ ﻦﻳﺮﺗﺪﻌﺘﺴﻣ زا ﻻﺎﺑ تارﺪﻴﻫﻮﺑﺮﻛ ﻪﺑ ﺐﺳﺎﻨﻣ ناﻮﻨﻋ چرﺎﻗ ﺪﺷر ياﺮﺑ ﻲﻌﻴﺒﻃ ﺮﺘﺴﺑ ﻦﻳﺮﺗ
يﺎﻫ
ﺖﺳا نﺎﻬﺟ رد از ﻦﻴﺴﻛﻮﺗﻼﻓآ )
12 ( ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻲﮔدﻮﻟآ .
ﻪﺘﺴﭘ و ناﺮﻳا ياﺮﺑ ﻞﻜﺸﻣ ﻚﻳ ﻚﺷ نوﺪﺑ ،ﺎﻫ يﺎﻫرﻮﺸﻛ ﺮﻳﺎﺳ
ﺖﺳا ﻪﺘﺴﭘ هﺪﻨﻨﻛﺪﻴﻟﻮﺗ )
2 ( فﺮﺼﻣ ﻲﮕﺸﻴﻤﻫ يﺎﺿﺎﻘﺗ . ،نﺎﮔﺪﻨﻨﻛ
زا يﺮﻴﮕﺸﻴﭘ يﺎﻬﻫار ﻪﻠﻤﺟ زا .ﺖﺳا ﻲﻳاﺬﻏ هدﺎﻣ ﺖﻣﻼﺳ و ﻲﻨﻤﻳا داﻮﻣ ﻲﻫدﻮﺗﺮﭘ ﺪﺷﺎﺑ ﻲﻣ چرﺎﻗ ﺪﺷر رﺎﻬﻣ ﻦﻴﺴﻛﻮﺗ ﻼﻓآ ﺪﻴﻟﻮﺗ ﻲﻳاﺬﻏ ﻦﻳا .ﺖﺳا ﻲﻳاﺬﻏ داﻮﻣ ﺪﻨﻳآﺮﻓ ﻲﻜﻳﺰﻴﻓ شور ﻚﻳ
ﺪﻴﻣﺎﻧ دﺮﺳ نﻮﻴﺳاﺰﻳرﻮﺘﺳﺎﭘ ﻲﻫﺎﮔ،ﺪﻨﻳآﺮﻓ ﻲﻣ ه
دﻮﺷ ) 13 ( ﻲﻫدﻮﺗﺮﭘ .
ناﺰﻴﻧﻮﻳ يﺎﻫﻮﺗﺮﭘ يژﺮﻧا ﺎﺑ اﺬﻏ ﻪﻛ ﺖﺳا يﺪﻨﻳآﺮﻓ ﻲﻳاﺬﻏ داﻮﻣ ﻲﻣ ﻪﻬﺟاﻮﻣ ﺪﺑﺎﻳ
) 13 ( )ﻂﺳﻮﺘﻣ يﺎﻫزود ﺎﺑ ﻲﻫدﻮﺗﺮﭘ . 10
- 1
يرﺎﮔﺪﻧﺎﻣ ﺶﻳاﺰﻓا و ﻲﺑوﺮﻜﻴﻣ ﻲﮔدﻮﻟآ فﺬﺣ ياﺮﺑ (يﺮﮔﻮﻠﻴﻛ ،نﺎﻴﻛﺎﻣ ،ﻲﻳﺎﻳرد يﺎﻫاﺬﻏ ،تﺎﺠﻳﺰﺒﺳ و هﻮﻴﻣ ﻞﻴﺒﻗ زا ﻲﻳاﺬﻏ داﻮﻣ ﺑرﺎﻛ هﺮﻴﻏ و تﺎﺟ ﻪﻳودا دراد دﺮ
) 14 ( ﺶﻟﺎﭼ ﻦﻳﺮﺘﻤﻬﻣ ﻪﻛ ﺎﺠﻧآ زا .
اﺬﻟ ﺖﺳا ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻢﺳ ﺖﻈﻠﻏ ندﻮﺑ ﻻﺎﺑ ،ناﺮﻳا ﻪﺘﺴﭘ تاردﺎﺻ
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
داﻮﻣ ﻲﺘﺷاﺪﻬﺑ ﺖﻴﻔﻴﻛ لﺮﺘﻨﻛ رد ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ ﻲﻳارﺎﻛ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ داﻮﻣ ﻦﺘﺷاﺪﻧ ﺰﻴﻧ و ﺎﻬﻧآ يرﺎﮔﺪﻧﺎﻣﺮﻤﻋ ﺶﻳاﺰﻓا و ﻲﻳاﺬﻏ ار سﺮﺘﺳد رد يژﺮﻧا ﻦﻳا دﺮﺑرﺎﻛ ﻢﻳﺪﺷ نآ ﺮﺑ ،ﻪﺘﻳﻮﻴﺘﻛاﻮﻳدار .ﻢﻴﻫد راﺮﻗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﺶﻟﺎﭼ ﻦﻳا ﻊﻓر ياﺮﺑ ﻖﻴﻘﺤﺗ ﻦﻳا زا فﺪﻫ
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ ناﺰﻴﻣ ﺮﺑ ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ ﺮﺛا ﻲﺳرﺮﺑ B1
ﻂﺳﻮﺗ
.ﺖﺳا هدﻮﺑ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ ﻲﺳرﺮﺑ شور
داﺪﻌﺗ ،ﺎﻫ ﻪﻧﻮﻤﻧ يزﺎﺳ هدﺎﻣآ رﻮﻈﻨﻣ ﻪﺑ 10
ﻪﺘﺴﺑ 200 زا ﻲﻣﺮﮔ
وﺮﺘﻜﻟا ﻮﺗﺮﭘ ﻂﺳﻮﺗ ﺲﭙﺳ ،ﺪﻳدﺮﮔ يﺪﻨﺑزرد و هدﺎﻣآ ﻪﺘﺴﭘ ﺎﺑ ﻲﻧ
زود 25 ﻞﻳﺮﺘﺳا يﺰﻛﺮﻣ ناﺮﻳا ﻲﺸﻫوﮋﭘ ﻊﻤﺘﺠﻣ رد يﺮﮔﻮﻠﻴﻛ
.ﺪﻧﺪﻳدﺮﮔ سﻮﻠﻳژﺮﭙﺳآ ﻪﻳﻮﺳ ﻲﭼرﺎﻗ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ يزﺎﺳ هدﺎﻣآ ﺖﻬﺟ ﻦﻴﺴﻛﻮﺗﻼﻓآ هﺪﻨﻨﻛﺪﻴﻟﻮﺗ سووﻼﻓ B1
ﻮﺘﻴﺗﻮﭘ ﺖﺸﻛ ﻂﻴﺤﻣ يور ﺮﺑ
)رﺎﮔآ زوﺮﺘﺴﻛد liofilchem
CAT#610102 تﺪﻣ ﻪﺑ (
10
رد زور 27 ﻲﺘﻧﺎﺳ ﻪﺟرد ﻟﻮﺗ ﺎﺗ ﺪﺷ هداد ﺖﺸﻛ داﺮﮔ
.ﺪﻨﻛ رﻮﭙﺳا ﺪﻴ
يﺎﻫ ﻲﻨﻠﻛ يور ﺮﺑ ﻦﻴﻳﻮﺗ هﺮﻄﻗ ﺪﻨﭼ ،چرﺎﻗ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ﻪﻴﻬﺗ ياﺮﺑ ﻪﻘﻴﻗد ﺪﻨﭼ زا ﺲﭘ و ﻪﺘﺨﻳر سﻮﻠﻳژﺮﭙﺳآ 2
- 1 ﺎﻫرﻮﭙﺳا زا پﻮﻟ
و هدﻮﻤﻧ ﻞﺣ يژﻮﻟﻮﻳﺰﻴﻓ مﺮﺳ رد ،ﻪﺘﺷادﺮﺑ رد ﺎﻫرﻮﭙﺳا داﺪﻌﺗ
درﻮﻣ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ نﺪﺷ ﻖﻴﻗر زا ﺲﭘ ﺶﻳﺎﻣزآ
زا هدﺎﻔﺘﺳا ﺎﺑ مﻻ
دﺮﮔ شرﺎﻤﺷ ،ﺮﺘﻣﻮﺘﻴﺳﻮﻤﻫ ﺖﻈﻠﻏ ﻪﺑ و هﺪﻳ
106 ﻲﻠﻴﻣ ﺮﻫ رد رﻮﭙﺳا
.ﺪﺷ هﺪﻧﺎﺳر ﺮﺘﻴﻟ ﻪﻧﻮﻤﻧ ﻪﺑ
يﺎﻫ 200 ناﺰﻴﻣ هﺪﺷ ﻞﻳﺮﺘﺳا ﻲﻣﺮﮔ 106
رﻮﭙﺳا چرﺎﻗ ﻪﺑ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ترﻮﺻ
هدﺎﻣآ هﺪﺷ ) 6 ﻲﻠﻴﻣ ﺮﻫ ءازا ﻪﺑ ﺮﺘﻴﻟ 200
.ﺪﻳدﺮﮔ ﺢﻴﻘﻠﺗ (ﻪﻧﻮﻤﻧ مﺮﮔ
ﺖﺧاﻮﻨﻜﻳ ﻊﻳزﻮﺗ رﻮﻈﻨﻣ ﻪﺑ ،ﻲﭼرﺎﻗ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ﺢﻴﻘﻠﺗ زا ﺪﻌﺑ .ﺪﻧﺪﺷ هدز ﻢﻫ ﻲﺘﺳد ترﻮﺻ ﻪﺑ ﺎﻫ ﻪﻧﻮﻤﻧ ،ﺎﻫرﻮﭙﺳا
ﻪﻧﻮﻤﻧ ﺲﭙﺳ ﻲﻫد ﻪﻌﺷا ﺖﻬﺟ يﺰﻛﺮﻣ ناﺮﻳا ﻲﺸﻫوﮋﭘ ﻪﺴﺳﻮﻣ ﻪﺑ ﺎﻫ
ﻪﻧﻮﻤﻧ ﻲﻫدﻮﺗﺮﭘ ﺰﻛﺮﻣ رد .ﺪﻨﺘﻓﺎﻳ لﺎﻘﺘﻧا رﺎﻤﻴﺗ هوﺮﮔ يﺎﻫ
ﻂﺳﻮﺗ
بﺎﺘﺷ نوﺮﺗودور نوﺮﺘﻜﻟا هﺪﻨﻫد TT200
10MeV ، ﺖﺤﺗ
يﺎﻫزود 1
، 3
، 5 و 7 ) ﻂﻴﺤﻣ يﺎﻣد رد يﺮﮔﻮﻠﻴﻛ 25
ﻪﺟرد
ﻲﺘﻧﺎﺳ ﻪﻧﻮﻤﻧ .ﺪﻧﺪﺷ ﻲﻫدﻮﺗﺮﭘ (داﺮﮔ ﺎﺑ ﻪﻬﺟاﻮﻣ نوﺪﺑ ﻢﻫ ﺪﻫﺎﺷ يﺎﻫ
.ﺪﻧﺪﻳدﺮﮔ يراﺪﻬﮕﻧ ﻂﻴﺤﻣ يﺎﻣد رد ﻲﻧوﺮﺘﻜﻟا ﻮﺗﺮﭘ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺖﻈﻠﻏ B1
ﻪﻧﻮﻤﻧ رد هﺪﺷﺪﻴﻟﻮﺗ ﻞﻳﺮﺘﺳا زا ﻞﺒﻗ ﺎﻫ
زود ﺎﺑ ندﺮﻛ 25
و ﻚﭙﻛ ﺢﻴﻘﻠﺗ زا ﺪﻌﺑ ﻦﻴﻨﭽﻤﻫ و يﺮﮔﻮﻠﻴﻛ
يﺎﻫزور رد ﻒﻠﺘﺨﻣ يﺎﻫزود رد ﻲﻫدﻮﺗﺮﭘ زا ﺪﻌﺑ
،ﺮﻔﺻ 210،180،90،60،30 .ﺖﻓﺮﮔ راﺮﻗ يﺮﻴﮔ هزاﺪﻧا درﻮﻣ
ﻪﻧﻮﻤﻧ ﻪﺑ يﺮﻴﮔ ﺖﺳﻮﭘ زا ﺲﭘ ﻪﺘﺴﭘ يﺎﻫ و هﺪﻣآرد ردﻮﭘ ترﻮﺻ
20 ﺎﺑ ﺎﻫ ﻪﻧﻮﻤﻧ زا مﺮﮔ 60
ﻲﻠﻴﻣ لﻮﻧﺎﺘﻣ ﺮﺘﻴﻟ 80
،ﺪﺻرد ﻚﻳ ﺎﺑ
) ﻻﺎﺑ ﺖﻋﺮﺳ ﺎﺑ ﻦﻛ طﻮﻠﺨﻣ 120
تﺪﻣ ﻪﺑ (ﻪﻘﻴﻗد رد رود 20
ﻪﻘﻴﻗد
.ﺪﻧﺪﺷ طﻮﻠﺨﻣ
ﻪﺑ هرﺎﺼﻋ ﻪﺑ ﺖﺳد
نﻮﻜﺳ ﺖﻟﺎﺣ رد ﺎﺗ ﺪﺷ هداد هزﺎﺟا هﺪﻣآ
ﻪﺑ ﺮﺘﻠﻴﻓ ﺬﻏﺎﻛ ﻖﻳﺮﻃ زا ﺲﭙﺳ و دﻮﺷ ﻞﻳﺪﺒﺗ زﺎﻓ ود ترﻮﺻ
Whatman No.1) هرﺎﺼﻋ .ﺪﻳدﺮﮔ جاﺮﺨﺘﺳا ﻲﻳور ﻲﻨﻏور زﺎﻓ (
ﻖﻴﻗر ﺮﻓﺎﺑ ﺎﺑ ﺎﺗ هﺪﻨﻨﻛ
4 اﺮﺑ اﺰﻳﻻا ﺖﻴﻛ زا هدﺎﻔﺘﺳا ﺎﺑ و ﻖﻴﻗر ﺮﺑ
ﻦﻴﺴﻛﻮﺗﻼﻓآ B1
Europroxima CAT# 5121AFB ) ﺎﺑ و (
اﺰﻳﻻا هﺎﮕﺘﺳد ﻂﺳﻮﺗ و ﺖﻴﻛ رد دﻮﺟﻮﻣ ﻞﻜﺗوﺮﭘ ﻪﺑ ﻪﺟﻮﺗ )ناﻮﺧ AWARENESS Technology ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺖﻈﻠﻏ (
هزاﺪﻧا .ﺖﺳا هﺪﺷ شراﺰﮔ مﺮﮔﻮﻧﺎﻧ ﺐﺴﺣ ﺮﺑ ﺮﻳدﺎﻘﻣ.ﺪﻳدﺮﮔ يﺮﻴﮔ
ﺑ ود ﺎﺑ ﻪﻧﻮﻤﻧ ﺮﻫ ياﺮﺑ نﻮﻣزآ .ﺪﺷ مﺎﺠﻧا راﺮﻜﺗ رﺎ
راﺰﻓا مﺮﻧ ﻂﺳﻮﺗ ﺎﻫرادﻮﻤﻧ ﻢﻴﺳﺮﺗ Excel
ﻪﺨﺴﻧ 2010 ﺪﺷ مﺎﺠﻧا
رﺎﻤﻴﺗ يﺎﻫ ﻪﻧﻮﻤﻧ رد ﻢﺳ ﺖﻈﻠﻏ ﻪﺴﻳﺎﻘﻣ و
و ﺪﻫﺎﺷ رد و ﺎﻫزود
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
راﺰﻓا مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﻒﻠﺘﺨﻣ يﺎﻫزور ﻪﺨﺴﻧ
16 ﺢﻄﺳ رد
)يراد ﻲﻨﻌﻣ 05
/
<0 نﻮﻣزآ ﻂﺳﻮﺗ (P Kruskal Wallis
ﻲﺳرﺮﺑ
ﺷ .ﺪ ﺎﻫ ﻪﺘﻓﺎﻳ
ﻲﮔدﻮﻟآ نوﺪﺑ ﻪﺘﺴﭘ ﻪﻴﻟوا يﺎﻫ ﻪﻧﻮﻤﻧ ﻪﻛ داد نﺎﺸﻧ ﺞﻳﺎﺘﻧ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ ناﺰﻴﻣ ﻦﻳﺮﺘﺸﻴﺑ و ﺪﻧدﻮﺑ ﻦﻴﺴﻛﻮﺗﻼﻓآ B1
رد
زور ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ 60
ﺎﺑ 85 / 164 ناﺰﻴﻣ ﻦﻳﺮﺘﻤﻛ و مﺮﮔ ﺮﺑ مﺮﮔﻮﻧﺎﻧ
زود ﻪﺑ طﻮﺑﺮﻣ هﺪﺷ ﺪﻴﻟﻮﺗ ﻢﺳ 7
رد رﺎﻤﻴﺗ يﺎﻫ هوﺮﮔ يﺮﮔﻮﻠﻴﻛ
يﺎﻫزور 60 و 180 .ﺪﺸﻧ ﻲﻳﺎﺳﺎﻨﺷ ﻲﻤﺳ ﭻﻴﻫ ﻪﻛ دﻮﺑ
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺮﻳدﺎﻘﻣ B1
يﺎﻫزور رد ﻒﻠﺘﺨﻣ يﺎﻫزود رد
،ﺮﻔﺻ 90،60،30 زور رد ﻲﻟو .ﺪﻨﺘﻓﺎﻴﻧ يراد ﻲﻨﻌﻣ ﺮﻴﻴﻐﺗ
يﺎﻫ 180 و 210
)ﺪﻳدﺮﮔ ﻲﻨﻴﺑﺎﻨﻴﺑ يراد ﻲﻨﻌﻣ ﻪﺑ ﻚﻳدﺰﻧ ﺢﻄﺳ ياراد 06
=/
.(
)لﺮﺘﻨﻛ يﺎﻫ ﻪﻧﻮﻤﻧ ﻦﻴﻨﭽﻤﻫ 55
/ 0
= ﺎﺑ هﺪﺷ رﺎﻤﻴﺗ يﺎﻫ ﻪﻧﻮﻤﻧ و (p
زود 7 )يﺮﮔﻮﻠﻴﻛ 09
p=/
ﺢﻄﺳ ياراد ﻒﻠﺘﺨﻣ يﺎﻫزور رد (
يﺎﻫزود رد و ﺪﻧدﻮﺑ يراد ﻲﻨﻌﻣ ﻪﺑ ﻚﻳدﺰﻧ 1
، 3 و 5 ﻲﻨﻌﻣ ﺮﻴﻴﻐﺗ
)ﺪﻨﺘﻓﺎﻴﻧ يراد 05
/
>0 .(p
ﺮﻳدﺎﻘﻣ يﺎﻫ ﻪﺘﺴﭘ ،ﻪﺘﺴﭘ ﻪﻴﻟوا ي ﻪﻧﻮﻤﻧ هوﺮﮔ ﻪﺳ رد هﺪﺷ ﺪﻴﻟﻮﺗ ﻢﺳ نوﺪﺑ و هﺪﺷ ﺢﻴﻘﻠﺗ )لﺮﺘﻨﻛ يﺎﻫ ﻪﺘﺴﭘ و هﺪﻳدﻮﺗﺮﭘ و هﺪﺷ ﺢﻴﻘﻠﺗ
يﺎﻫرادﻮﻤﻧ رد(ﻲﻫدﻮﺗﺮﭘ 1
و 2 ﺪﺻرد ﻦﻴﮕﻧﺎﻴﻣ .ﺖﺳا هﺪﺷ هدروآ
يﺎﻫزور رد رﺎﻤﻴﺗ يﺎﻫ ﻪﻧﻮﻤﻧ رد ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺮﻳدﺎﻘﻣ ﺶﻫﺎﻛ يﺎﻫزود رد يراﺪﻬﮕﻧ ﻒﻠﺘﺨﻣ 1
، 3 ، 5 و 7 ﻮﻠﻴﻛ ﺐﻴﺗﺮﺗ ﻪﺑ يﺮﮔ
84 / 38 ، 79 / 48 ، 50 / 53 ، 17 / 77 .دﻮﺑ ﺪﺻرد
رادﻮﻤﻧ 1 : ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺖﻄﻠﻏ تاﺮﻴﻴﻐﺗ B1
يراﺪﻬﮕﻧ يﺎﻫزور و رﺎﻤﻴﺗ يﺎﻫزود ﻚﻴﻜﻔﺗ ﻪﺑ
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
Day
0 30 60 90 120 150 180 210
Density
0 20 40 60 80 100 120 140 160 180
Control Dose 1 Dose 3 Dose 5 Dose 7
رادﻮﻤﻧ
2 : يرادرﺎﺒﻧا هرود رد ﻒﻠﺘﺨﻣ يﺎﻫزود رد ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺖﻈﻠﻏ
يﺮﻴﮔ ﻪﺠﻴﺘﻧ و ﺚﺤﺑ
ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ ﺪﻧور ﺮﺑ ﻲﻧوﺮﺘﻜﻟا ﻮﺗﺮﭘ ﺮﺛا ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد B1
ﻒﻠﺘﺨﻣ يﺎﻫزور رد ﻪﺘﺴﭘ يرادرﺎﺒﻧا و ﻒﻠﺘﺨﻣ يﺎﻫزود رد ﺖﻈﻠﻏ يﺮﻴﮔ هزاﺪﻧا زا ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ .ﺖﻓﺮﮔ راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ ﻒﻠﺘﺨﻣ يﺎﻫزود رد ﻢﺳ ﺮﻳدﺎﻘﻣ ﺶﻫﺎﻛ ،داد نﺎﺸﻧ ﻦﻴﺴﻛﻮﺗﻼﻓآ هﺎﻣ ﺖﻔﻫ ﻞﻗاﺪﺣ ﺎﺗ ﺪﻧاﻮﺗ ﻲﻣ ،ﻲﻫدﻮﺗﺮﭘ ﺮﺛا ﺮﺑ يراﺪﻬﮕﻧ هرود رد ﻴﺑ ﺮﻴﺧﺎﺗ ﻪﺑ ار ﻢﺳ ﺪﻴﻟﻮﺗ ﺎﺒﻗﺎﻌﺘﻣ و چرﺎﻗ ﺪﺷر ناﺰﻴﻣ اﺬﻟ .دزاﺪﻨ
ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد ﻪﻛ .ﺪﺷﺎﺑ ﻲﻣ ﺮﻔﺻ ﻪﺑ ﻚﻳدﺰﻧ يﺪﻴﻟﻮﺗ ﻦﻴﺴﻛﻮﺗﻼﻓآ .ﺪﺷﺎﺑ ﻲﻣ ﺮﺘﻤﻛ رﺎﻴﺴﺑ ناﺰﻴﻣ ﻦﻳا لﺮﺘﻨﻛ هوﺮﮔ
رد ﺎﻣﺎﮔ و ﻲﻧوﺮﺘﻜﻟا يﺎﻫﻮﺗﺮﭘ ﻂﺳﻮﺗ ﺎﻫ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺐﻳﺮﺨﺗ ﻂﺳﻮﺗ يا ﻪﻌﻟﺎﻄﻣ .ﺖﺳا هﺪﺷ ﻲﺳرﺮﺑ تﺎﻌﻟﺎﻄﻣ ﻲﻀﻌﺑ Supriya
) 2014 ( ﺗ و ﻲﭼرﺎﻗ ﻲﻳادز ﻲﮔدﻮﻟآ ناﻮﻨﻋ ﺖﺤﺗ يرﺎﮔﺪﻧﺎﻣ ﺖﻳﻮﻘ
ﻪﻧاد .ﺪﺷ مﺎﺠﻧا ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ ﻂﺳﻮﺗ ﺎﻴﺑﻮﻟ ﻪﭙﻟ يﺎﻫ
ﺶﺑﺎﺗ هﺪﺸﻧ ﺎﻴﺑﻮﻟ
ﻚﺸﺧ ﺖﺤﺗ يﺎﻫﺎﻴﺑﻮﻟ و ﺶﺑﺎﺗ
ﺎﺑ زود ﻮﺗﺮﭘ ﻲﻧوﺮﺘﻜﻟا 5
/ 2
، 5
، 10
و 15
،يﺮﮔﻮﻠﻴﻛ رد
لﻮﻃ 0
، 3 و 6
،هﺎﻣ رد ﻂﻳاﺮﺷ ﻲﻫﺎﮕﺸﻳﺎﻣزآ و هﺪﺷ هﺮﻴﺧذ
ﺖﺤﺗ ﺮﻈﻧ راﺮﻗ ﺪﻨﺘﻓﺮﮔ . ﻪﻧﻮﮔ زوﺮﺑ و عﻮﻗو ﺶﻫﺎﻛ
رد چرﺎﻗ يﺎﻫ ﺮﮔا .ﺪﻧدﻮﺑ زود ﻪﺑ ﻪﺘﺴﺑاو ﺎﻫ ﻪﻧﻮﻤﻧ
ﻪﭼ ﻦﻴﺴﻛﻮﺗﻼﻓآ
) B1
و B2
( ﺶﺑﺎﺗ ﺖﺤﺗ و لﺮﺘﻨﻛ يﺎﻫ ﻪﻧاد رد 5
/ 2 و 5 ،يﺮﮔﻮﻠﻴﻛ
ﺮﻳز ﺢﻄﺳ ﻞﺑﺎﻗ )ﺺﻴﺨﺸﺗ
<2 رد ﻲﻟو دﻮﺑ (مﺮﮔ ﺮﺑ مﺮﮔﻮﻧﺎﻧ
يﺎﻫزود 10
و 15 ﺪﺸﻧ ﻲﻳﺎﺳﺎﻨﺷ ﻲﻤﺳ ﭻﻴﻫ يﺮﮔﻮﻠﻴﻛ )
15 ( .
ﻂﺳﻮﺗ يا ﻪﻌﻟﺎﻄﻣ Bhat
) 2014 د نﺎﺸﻧ ﻲﺑآﺮﻓﻮﻠﻴﻧ يﺎﻫ ﻪﻧاد رد ( دا
سﻮﻠﻳژﺮﭙﺳآ يﺎﻫ چرﺎﻗ ﻞﻣﺎﻛ ﻲﻳادز ﻲﮔدﻮﻟآ ياﺮﺑ مزﻻ زود ﻪﻛ ﻦﻴﺴﻛﻮﺗﻼﻓآ و 15
- 10 ﺶﻫﺎﻛ ﺎﻳ ﺐﻳﺮﺨﺗ .دﻮﺑ يﺮﮔﻮﻠﻴﻛ
زود رد ﻦﻴﺴﻛﻮﺗﻼﻓآ 10
داد خر يﺮﮔﻮﻠﻴﻛ )
13 ( يا ﻪﻌﻟﺎﻄﻣ رد .
ﻲﻤﻛ ﺮﻳدﺎﻘﻣ ﻪﻴﻟوا يﺎﻫ ﻪﻧﻮﻤﻧ رد ،مادﺎﺑ درآ رد ﺮﮕﻳد )ﻦﻴﺴﻛﻮﺗﻼﻓآ 5
/ 0 ﻤﻧ ﻦﻳا رد .ﺪﺷ اﺪﻴﭘ (مﺮﮔ ﺮﺑ مﺮﮔوﺮﻜﻴﻣ ﺎﻫ ﻪﻧﻮ
ﻪﻬﺟاﻮﻣ زا ﺲﭘ زود ﺎﺑ
5 / 1 ﭻﻴﻫ ،ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ يﺮﮔﻮﻠﻴﻛ
ﺪﺸﻧ ﻲﻳﺎﺳﺎﻨﺷ ﻲﻨﻴﺴﻛﻮﺗﻼﻓآ )
16 ( .
ﻦﻴﻨﭽﻤﻫ Rogovschi
) 2007 ﺶﻫﺎﻛ ﺮﺑ ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘﺮﺛا (
يﺎﻫ ﻦﻴﺴﻛﻮﺗﻼﻓآ B1
و B2
ﺮﺑ رﺎﮔآ تﺎﻨﻛﻮﻛ ﺖﺸﻛ ﻂﻴﺤﻣ رد
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
زا ﺪﻌﺑ سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ 7
ﺎﺑ ﻲﻫدﻮﺗﺮﭘ زا ﺲﭘ ،ﺖﺸﻛ زور
زود 5 ﺶﻫﺎﻛ يﺮﮔﻮﻠﻴﻛ 5
/ 75 .داد نﺎﺸﻧ ار ﻦﻴﺴﻛﻮﺗﻼﻓآ يﺪﺻرد
زود رد ﻪﻛ ﻲﻟﺎﺣ رد 50
ﻲﻳﺎﺳﺎﻨﺷ ﻲﻨﻴﺴﻛﻮﺗﻼﻓآ ﭻﻴﻫ يﺮﮔﻮﻠﻴﻛ
ﺪﺸﻧ ) 17 (.
رد ﻞﻴﺧد ﻂﻳاﺮﺷ ﻪﺑ ﺖﺳا ﻦﻜﻤﻣ تﺎﻌﻟﺎﻄﻣ ﺞﻳﺎﺘﻧ ﻦﻴﺑ توﺎﻔﺗ ﻪﻠﻤﺟ زا ﻲﻳاﺬﻏ هدﺎﻣ بآ ﺖﻴﻟﺎﻌﻓ و ﺖﺑﻮﻃر .ﺪﺷﺎﺑ ﻂﺒﺗﺮﻣ ﻲﻫدﻮﺗﺮﭘ
ﺮﭘ ﺎﺑ ﻲﻫدﻮﺗﺮﭘ ﺪﻨﻳآﺮﻓ رد هﺪﻤﻋ ﺶﻘﻧ ﻪﻛ ﺖﺳا ﻲﻳﺎﻫرﻮﺘﻛﺎﻓ يﺎﻫﻮﺗ
ناﺰﻴﻣ ﻲﻠﻛ رﻮﻃ ﻪﺑ .ﺪﻧراد ﺎﻫ چرﺎﻗ ياﺮﺑ ناﺰﻴﻧﻮﻳ ﺪﻴﻟﻮﺗ چرﺎﻗ ﺪﺷر
ﺪﻴﻟﻮﺗ و ﻢﺳ هﺪﻨﻨﻛ ،ﻲﻜﻳﺰﻴﻓ ﺐﻟﺎﻏ ﻂﻳاﺮﺷ ﻪﺑ ﻦﻴﺴﻛﻮﺗﻼﻓآ
ﻲﻳﺎﻤﻴﺷﻮﻴﺑ و ﻲﻄﻴﺤﻣ ﺖﺴﻳز ،ﻲﻜﻳژﻮﻟﻮﻴﺑ ﻲﮕﺘﺴﺑ
دراد ) 18 ( .
ﻦﻜﻤﻣ هﺪﺷ ﺪﻴﻟﻮﺗ ﻢﺳ ناﺰﻴﻣ رد توﺎﻔﺗ ﻪﻛ هداد نﺎﺸﻧ تﺎﻌﻟﺎﻄﻣ .ﺪﺷﺎﺑ ﻪﺘﺷاد ﻲﮕﺘﺴﺑ ﻲﻳاﺬﻏ لﻮﺼﺤﻣ ﻲﻳﺎﻤﻴﺷﻮﻴﺑ ﻂﻳاﺮﺷ ﻪﺑ ﺖﺳا سووﻼﻓ سﻮﻠﻳژﺮﭙﺳآ سﺮﺘﺳد ﻞﺑﺎﻗ تارﺪﻴﻫﻮﺑﺮﻛ ﻊﺒﻨﻣ ﻦﻴﻨﭽﻤﻫ ﺖﺳا ﺮﺛﻮﻣ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ رد
) 18 ( ﻪﺘﺴﭘ يﺎﻫ ﻪﻧﻮﻤﻧ نداد راﺮﻗ
ﻮﺗﻼﻓآ ﺪﻴﻟﻮﺗ زا ﻞﺒﻗ ﻲﻧوﺮﺘﻜﻟاﻮﺗﺮﭘ ضﺮﻌﻣ رد زا ﺎﺑ ﺪﻧاﻮﺗ ﻲﻣ ﻦﻴﺴﻛ
يﺮﻴﮕﺸﻴﭘ ﻦﻴﺴﻛﻮﺗﻼﻓآ ﻢﺳ ﺪﻴﻟﻮﺗ زا ﻢﺳ ﺪﻟﻮﻣ چرﺎﻗ ندﺮﺑ ﻦﻴﺑ .ﺪﻳﺎﻤﻧ ﺮﻜﺸﺗ و ﺮﻳﺪﻘﺗ
،يﺰﻛﺮﻣ ناﺮﻳا ﻲﺸﻫوﮋﭘ ﻊﻤﺘﺠﻣ يرﺎﻜﻤﻫ زا ﻪﻠﻴﺳو ﻦﻳﺪﺑ ﺰﻛﺮﻣ
ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻞﺜﻣ ﺪﻴﻟﻮﺗ ﻲﻟﻮﻜﻟﻮﻣ تﺎﻘﻴﻘﺤﺗ تﺎﻣﺪﺧ و
ﺎﻣ ﻪﻌﻟﺎﻄﻣ ﻦﻳا مﺎﺠﻧا رد ﻪﻛ يداﺮﻓا مﺎﻤﺗ و دﺰﻳ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ ار
.ددﺮﮔ ﻲﻣ ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ ،ﺪﻧدﻮﻤﻧ يرﺎﻳ
ﻊﻓﺎﻨﻣ دﺎﻀﺗ
ﻲﻐﻓﺎﻨﻣ دﺎﻀﺗ ﻪﻧﻮﮔ ﭻﻴﻫ ﻪﻛ ﺪﻧراد ﻲﻣ مﻼﻋا ﻪﻟﺎﻘﻣ ﻦﻳا نﺎﮔﺪﻨﺴﻳﻮﻧ .دراﺪﻧ دﻮﺟو
References
1-Hepsag F, Golge O, Kabak B. Quantitation of aflatoxins in pistachios and groundnuts using HPLC-FLD method. Food Control 2014;38:75-81.
2-Saremi H, Okhovat M. Effect of aflatoxin produced by Aspergillus flavus in reduction of our pistachio marketing all over the world 2008;2(3):13-19.
3-Dini A, Khazaeli P, Roohbakhsh A, Madadlou A, Pourenamdari M, Setoodeh L, et al. Aflatoxin contamination level in Iran's pistachio nut during years 2009–2011. Food Control 2013;30(2):540-4. 4-Al-Moghazy M, Boveri S, Pulvirenti A. Microbiological safety in pistachios and pistachio containing products. Food Control 2014;36(1):88-93.
5-Zain ME. Impact of mycotoxins on humans and animals. Journal of Saudi Chemical Society 2011;15(2):129-44.
6-Mahoney NE, Rodriguez SB. Aflatoxin variability in pistachios. Applied and environmental microbiology 1996;62(4):1197-202.
ود ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﻪﻣﺎﻨﻫﺎﻣ دﺰﻳ ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
لﺎﺳ ﺎﺷ ﻢﻫدﺰﻧ
، هرﺎﻤﺷ ﺮﻴﺗ و دادﺮﺧ،مود 1396
7-Klich MA. Environmental and developmental factors influencing aflatoxin production by Aspergillus flavus and Aspergillus parasiticus. Mycoscience 2007;48(2):71-80.
8-Zaini F, Yousefi S, Dadgar S, Safara M. Aflatoxin production by Aspergillus flavus isolates from green–tiger shrimps (Penaeus semisulcatus). Iranian Journal of Microbiology 2009;1(4):18-22. 9-Karami-Osboo R, Mirabolfathy M, Kamran R, Shetab-Boushehri M, Sarkari S. Aflatoxin B1 in maize harvested over 3 years in Iran. Food Control 2012;23(1):271-4.
10-Rahimi P, Sharifnabi B, Bahar M. Detection of aflatoxin in Aspergillus species isolated from pistachio in Iran. Journal of phytopathology 2008;156(1):15-20.
11-Georgiadou M, Dimou A, Yanniotis S. Aflatoxin contamination in pistachio nuts: A farm to storage study. Food control 2012;26(2):580-6.
12-Mohamadi moghadam M, Sobhanipour A, Hokmabadi A. The relationship between the growth of aflatoxin-causing fungus Aspergillus flavus and aflatoxin B1 production of nutrients and protein in the brain with different varieties of pistachio. Journal of Horticultural Science 2011;1389(2). 13-Bhat R, Sridhar K, Karim A. Microbial quality evaluation and effective decontamination of nutraceutically valued lotus seeds by electron beams and gamma irradiation. Radiation Physics and Chemistry 2010;79(9):976-81.
14.Lung H-M, Cheng Y-C, Chang Y-H, Huang H-W, Yang BB, Wang C-Y. Microbial decontamination of food by electron beam irradiation. Trends in Food Science & Technology 2015;44:66-78.
15.Supriya P, Sridhar K, Ganesh S. Fungal decontamination and enhancement of shelf life of edible split beans of wild legume Canavalia maritima by the electron beam irradiation. Radiation Physics and Chemistry 2014;96:5-11.
16-Lanza C, Mazzaglia A, Paladino R, Auditore L, Barnà R, Loria D, et al. Characterization of peeled and unpeeled almond (Prunus amygdalus) flour after electron beam processing. Radiation Physics and Chemistry 2013;86:140-144.
17-Rogovschi VD, Aquino S, Nunes TC, Gonçalez E, Corrêa B, Villavicencio A, editors. Use of electron beam on aflatoxins degradation in coconut agar. Proceedings of the International Nuclear Atlantic Conference, INAC, Rio de Janeiro, Brazil 2009.
18-Ghanem I, Orfi M, Shamma M. Effect of gamma radiation on the inactivation of aflatoxin B1 in food and feed crops. Brazilian Journal of Microbiology 2008;39(4):787-91.
