JOURNAL OF SCIENCE OF HNUE DOI: 10.18173/2354-1059.2017-0007 NaturalSci. 2017, Vol. 62, No. 3. pp. 51-59
This paper is available online at http;//stdb.hnue.edu.vn
T O N G HQtP VA D A C TRU'NG M A N G H Y D R O X Y A P A T I T PHA T A P D O N G T R E N NEN T H E P K H O N G G I 316L
V o T h i H a n h ' , P h a m Thi N a m ^ v a D i n h Thi M a i Thanh-*
Khoa Khoa hpe Co bdn, Trudng Dgi hoc Md - Dia Chat ' Vien Ki thugt nhiet ddi, Vien Hdn ldm Khoa hoc vd Cdng nghe Viet Nam Trudng Bgi hpc Khoa hoc vd Cdng nghe Hd Ndi, Vien Hdn ldm Khoa hoc vd Cdng nghe Viet Nam
Tom tat. Mang hydroxyapatil pha tap dong (CuHAp) duac tong hgp bang phuong phap trao doE ion giiia mang hydroxyapatil (HAp) voi dung dich Cu(N0])2. Nhung yeu to nong dp ion Cu"* va thoi gian anh hucmg den qua trinh trao doi ion da dirge khao sat. Ket qua cho thay ion Cu'* da thay the ion Ca * trong cau tnic ciia HAp de tao mang CuHAp co ti le nguyen tii Cu/Ca = 0,0124, ti le (Cu+Ca)/P = 1,571. KSt qua phan tich hinh thai hpc, cau tnic pha va thanh phan pha cho thay mang CuHAp co cau tnic tinh the, dan pha va sir co m|t cua Cu^* da lam thay doi hinh thai hoc va lam giam kich thuoc ciia CuHAp so vai HAp. Ngoai ra ket qua nghien ciiu cung cho thay dpng hoc qua trinh trao doi ion tuan theo phan umg gia bac hai vdi dung lugng trao doi ion o trang thai can bang 11,99 mg Cu'*/g HAp va hang so loc dp phan ling la 3,57 x lO"'^ g/mg.phiil. Mang CuHAp co kha nang khang khuan doi vol chung khuan P. aei-ugimosa voi ban kinh vong lic che dai 3 mm
Tu-khda: Thip khong gi 316L, mang hydroxyapalile pha tap dong (CuHAp), trao doi ion.
1. Md
6hu
H y d r o x y a p a l i l e ( H A p ) la thanh p h i n chinh trong x u o n g l u nhien, HAp chiem khoang 25 - 7 5 % theo trgng lugng va 35 - 6 5 % theo the tich [1]. HAp long hgp co thanh ti l e C a / P = 1,67, tuong l u nhu trong x u a n g tu nhien, co kha nang tuang thich sinh hgc tot nen dugc nghien cuu rgng rai va u n g dung trong ITnh vue y sinh [2, 3], Man^ HAp dugc phii len be mat kim loai va hgp kim nhu Ti. thep khong gi 3 1 6 L . . . d e lam vat lieu cay ghep x u a n g [4, 5]. Khi cay ghep. mang HAp CO kha nang dinh hinh. tao lien ket chat che giiia x u a n g cua vat chii va val lieu e5y ghep, thiic d i y qua trinh liSn x u a n g [6]. Tuy nhien, van de nhiem trung sau phau thuat dugc quan lam nhat sau khi dy ghep vao cg the, do do cac nha khoa hpc da rat quan tam dua cac tac nhan khang khuan vao vat lieu c i y ghep.
Tac nhan khang k h u i n vo ca gom cac ion dong, bac, kem dugc cac nha khoa hpc chii y vi su 6n dinh va tinh an loan [1], chiing co tac dung lam giam dp bam dinh cua vi khuan tren be mat v | l lieu va lam lirc ch6 su lang truang ciia vi khuan [7]. V^t lieu Ti chijra 2 % ham lugng dong v's kh6i lugng CO the chong d u g c 5 7 % khuan E.Coli va 7 9 % khuSn S. Aureus [50). Hoat dgng khang khuSn cua hgp c h i t HAp pha lap d6ng da dugc Ihti nghiem. k6t qua cho thiy bpl HAp co pha lap Cu vdi cong thuc Ca,o.,Cu,(PO^)ft(OH)2 (x = 0.2) co kha nang eh6ng duge 9 8 % vt khuan S. Aureusis
Ngay nhan bai: 19/2/2017. Ngay nhan dang: 20/3/2017.
Tac gia lien he: Vo Thj Hanh, e-mail: [email protected]
Vo Thj H?nh, Ph?im TTii Nam va Dinh Thi Mai Thanh
va bpt HAp pha tgp Cu, F iing vai cong thiic Ca,o,,Cu«(P04)6(F)2 (x = 0,15 - 0,5) co lh£ ch6ng dugc 100% vi khuan E.Coli [1]. Ngoai ra, vai ham lugng nho Cu eon co tac dung tang cuong su trao doi chat va b'ch thich te bao xuang phat trien [6].
Trong bai bao nay. chiing toi gidi thieu kel qua nghien cuu long hgp mang HAp pha tap Cu (CuHAp) tren nen thep khong gi 316L bSng phuang phap trao doi ion giiJa mang HAp vdi dung dich chura Cu *. Cac yeu to anh huang den qua trinh nhu nong dp ion Cu ', thai gian tiep xiic dl dugc nghien cuu. Mang CuHAp tao ra dugc nghien cuu thanh phan, eac dac trung hoa ly bang phuang phap IR, X-ray. SEM, AAS va hoat tinh khang khuan ehiing P.aerugimosa bang phuang phap khuech tan dia thach.
2. Noi dung nghien cuu 2.1. Thu'c nghiem
* Tong hop dien hda ming HAp tren nen thep khong gi 316L
Mau thep khong gi 316L (TKG 316L) vai thanh phin hoa hpc: 0,27% Al; 0,17% Mn;
0,56% Si; 17.98% Cr; 9,34% Ni, 2,15% Mo; 0,045% P; 0,035% S va 69,45% Fe co kich thuae la 1 >< 10 >< 0,2 cm, dugc phii epoxy degiai han dien tich lam vipc Icm^. Sau do mau dugc phu mang HAp bing phufong phap quel the calot a khoang quet the 0 ~" -1,7 V/SCE; nhiet dp: 50 "C, 5 lan quet vai t6c do quel 5 mV/s trong dung dich chiia Ca(NO3)2 3.10"^M + NH4HzP0.) 1,8.10'^ M + NaNO3 6.10-^M.
* Tong hep mdng CuHAp
De xac dinh nong dp Cu^* va thai gian liep xiic thich hgp cho qua trinh tong hgp mang CuHAp, vat lieu TKG 316L phii mang HAp (HAp/TKG316L) eo khoi luong 2,45 mg dugc ngam trong 4 mL dung dich chua muoi CuCNGs); vai nong dp khac nhau: 0,005; 0.01; 0,02; 0,05 va 0,1M; va thcri gian dugc khao sat: 2,5; 5; 10; 20; 30; 60 va 80 phiit a nhipt dp phong, Sau do vat lieu dugc lay ra, nJa sach bang nuac cat va de kho trong khong khi. Phan dung dich con lai sau khi ngam dung de xac dinh nong dp Cu^* con lgi bang phuong phap hap thy nguyen ni (AAS), tir do xac dinh dung lugng trao doi ion theo phuang trinh sau:
(1)
trong do, Q la dung lugng trao doi ion (mg/g), Co la nong dp ban dau ciia Cu^* (mol/L), C la nflng dp Cu^"^ con lgi sau khi ngam (mol/L), V la the lich dung dich (L), m la khoi lugng mang HAp (g), M la khoi lugng moi cua Cu (g).
Dgng hpc ciia qua trinh trap doi ion Cu^* duge xac dinh theo hai mo hinh dpng hgc: gia bac I (phucmg trinh 2) va gia bgc hai (phuang trinh 3) [8]:
in(a-a)=ina-*,-' (2)
J___\_ 1
Q,~Q/^k,.Ql (3) trong do: Qe va Q, lan lugt la dung lugng trao d6i ion a trsing thai can bing va cr thai di6m I (mg/g),
k], k2- hing s6 toe dp cua phan ung gia bac 1 (phut"') va gia bac 2 (g/mg.phiit).
Tong hap vd ddc n-img mang Hydroxtrapatit pha tgp ddng tren nen thep khong ^ 3i6L
* Dac diem mang CuHAp
Mang CuHAp dugc xac djnh thanh phan cac nhom chuc bing phuang phap IR Iren may FT- IR 6700 cua hang Nieolel vai ki diuat ep vien KBr. Thanh phin pha ciia CuHAp dugc ghi tren may SIEMENS D5005 Bruker-Germany, vdi cac difeu kien hue xa Cu-Ka, budc song X = 1,5406 A, cuong dg dong dien bing 30 rnA, dien ap 40 kV, goc quel 29 U-ong khoang 10" ^ 70°, l6c do quet 0,0307giay. Hinh thai hpe bS mat mang CuHAp xae dinh bing phucmg phap SEM tren thiet bi dien tii quel Hitachi S4800 (Nhat Ban). Thanh phin cac nguyen l6 Ca va Cu co trung mang CuHAp dugc xac dinh bang phuang phap quang pho hap thu nguyen lii tren may Perkin - Elmer 3300 va nguyen l6 P dugc xac dinli bing phuang phap UV-VIS tren may CINTRA.
Tir gian do nhieu xa tia X co the tinh dugc duang kinh trung binh ciia linh the CuHAp theo cong thuc Scherer [9]:
n _ OM (4)
trong do: D- duang kinh tinh the (nm), >.- buac song (nm); o day X = 0,15406 nm, B (rad): do rgng pic tai nua chieu cao ciia pic dac tnmg, B (rad) dugc tinh lir B (") theo cong thiic sau: B (rad)
= B C) >< n/l 80; 9 - goc nhieu xa C).
HAp CO mang linh thfi he luc giac vai a = b # c, a = p = 90", y = 120". Tir gian d6 nhieu xa tia X xac dinh duge gia tri khoang each giira cac mat phang linh the (d), ttrdo xac djnh duac gia tri hing so mang a, b, c theo cong thue 5 [10]
1 ^^ii-'*kh^k')^,, ^j^
d^ a^ c^
* Thu- hoat tinh khdng khudn bdngphirangphdp khuech tdn dTa thach
Mang HAp va CuHAp dugc eao bgt va ep thatili vien co chieu day 1 mm va duang kinh 10 mm, sau do dugc hap khii trung a 150 "C trong 30 phut. Chung vi khuan P.aerugimosa dugc su dung de thii nghiem hoat tinh khang khuan. dugc nuoi cay lii ong chung goc, tren moi truong LB dac lai 37 °C, u qua dem. KT thuat khuech tan dugc thuc hien bing each do thach vao cac dta Petri de tao thanh lop day 4 mm va cay day dac ciia cac vi sinh vat thii nghiem de eo dugc toe do tang trudng semiconfluent. Cac mau HAp va CuHAp duge bo tri tren be mat thach va ii trong 24 gid d 37 °C.
Hoat tinh uc ch6 khuan dugc danh gia bang each do ban kinh (BK) vong ue che vi sinh vat theo cdng thiic; BK (mm) = D-d, trong do D: dudng b'nh vdng vo khuSn va d: dudng kinh lo khoan thach. Thi nghiem dugc lap lai ba lan va lay gia tri ban kinh trung binh.
2.2. K i t qu^ va thao lu$n 2.2.L Anh htrdng cua nong do Cu^^
K6l qua khao sal anh hudng nong do ban dau ctia Cu^* tir 0,005 M - 0,1 M den dung lugng irao ddi ion Uifi hien tren Hinh 1. Nong dp Cu^^ trong dung dich tang iW dung lugng trao ddi ion tang. Dung lugng trao doi ion tang nlianh tu 4.18 mg/g len 10,66 mg/g khi ndng dp Cu^*tang tir 0.005 M den 0,02 M. sau do tang khong nhieu khi lang ndng dd Cu^*len 0,05M va 0,1 M (dung lugng tang len tuang iing 12.56 mg/g va 13.43 mg/g) vi qua trinh trao doi ion da dat ldi trang thai can bing. Do dd, dung dich Cu^* co ndng dp 0,02M dugc sir dyng de long hgp mang CuHAp.
Vo Thj Hanh, Ph^m Thi Nam va Dinh Thi Mai Thanh
C,( Cii"/M|
Hinh J. Su- bien ddi dung luang (Q) trao ddi ion Cu^*cutt mdng HAp theo ndng do Cu^*
vdi thdi gian ngam 30 phut d nhiet dp 25 °C
I (phOI)
Hinh 2. Su bien ddi dung luang (Q) trao doi ion Cu'*cua mdng HAp theo thdi gian tiep xuc trong dung dich Cu^* 0,02Mdnhiet do 25 "C 2.2.2. Anh hutfng cua thoi gian tiep xiic
Su bien doi dung luang trao doi ion Cu^* ciia m^ng HAp theo thdi gian dugc the hien tren Hinh-2. Trong khoang Ihdi gian khao sat tir 2,5 den 80 phiit, dung lugng trao ddi ion tang nhanh d 30 phut dau (tang tir 8,11 mg/g len 10,96 mg/g), sau do dung lugng Uiaydoi khdng dang ke trong khoang thdi gian tir 30 den 80 phiit (tir 10,96 mg/g den 11,69 mg/g) Do vay, thdi gian 30 phiit duge coi la thdi diem can bang va dugc lua chpn lam thdi gian tdng hgp mang CuHAp.
2.2.3. Nghien ciiii cau true, thanh phan va hinh thai hoc ciia mang CuHAp Mang CuHAp dugc long hgp bang each ngam mang HAp 30 phiit trong dung dich Cu^' 0,02 M, sau do mlu nia sach, lam kho va dugc xac djnh cau tnic, thanh phan va hinh thai hpe bang cac phuang phap IR, Xray, SEM, UV-VIS. AAS.
* Hdng ngogi (IR)
Hinh 3 gidi thieu phd hdng ngoai cua mang HAp va mang CuHAp trong khoang budc sdng tir 4000 cm'' den 400 em'' Pho IR ciia hai mau deu cd hinh dang tuong ty nhau va ed cac pic dac tmng cho dao dpng ciia cac nhdm chiic trong phan lu HAp
Hinh 3. Phd IR ciia mdng HAp vd mdng CuHAp
Cac pic d sd sdng 1034; 962; 602 va 565 cm"' dac Irung cho nhdm PCi''". Trong dd, dao ddng keo dai bit ddi xiing ciia P-0 dac trung bdi 2 pic lai vi tri 1034 cm'' va 962cm"' (ujb va Us^); dao
Tong hpp vd dgc tiimg mdng Hydroxyapatil pha tgp ddng ti-en nen thip khong gi 316L dpng udn khdng ddi xung ciia 0-P-O o 602 cm'' va 565 cm'' (u^i, va U41). Pic hip phu d sd sdng 3430 va 1643 cm'' dac trung cho dao ddng hda tri va dao ddng bien dang eiia nhdm OH', Ngoai ra, tren phd mang HAp edn cd dai hap phu o vi tri 1384 em'' dae trung cho nhdm NOj", ion NOt' cd mat trong dung dich long hgp mang HAp. Tuy nhien, mang HAp sau khi ngSm 30 phut trong dung dich Cu'* thi ion NOj" khuyech tan vao dung dich nen trong phd IR ciia mang CuHAp khdng cd pic cua nhdm NO3".
* Thdnh phan mdng CuHAp
Ket qua phan lich thanh phan cac nguyen td Ca va Cu cd irong mang CuHAp dugc xac dinh bang phuang phap AAS va thanh phan nguyen td P dugc xac dinh bing phuang phap UV-VIS the hien Irong Bang I.
Bdng 1. Hdm luang % cdc nguyen td Ca, Cu, P cd trong mdng CuHAp
Mang HAp Mang CuHAp
% Khoi luQ'ng nguyen to Ca
33,2 Ca 34,173
P 1 16,8
Cu P 0,677 17,062
Tl le nguyin tir
- --
Cu/Ca Ca/P 1,532 (Ca + Cu)/P 0,0124 1 1,571 Mang CuHAp cd ham lugng ion Cu'' chi6m 0,677% vdi ti le Cu/Ca = 0,0124 va ti le (Ca+ Cu)/P
= 1,571, xap xi ti lp cua Ca/P trong mang HAp ban dau (1,532). Kel qua nay chiing Id ion Cu^' da thyc hien phan ung trao doi ion vdi Ca'*^ de tao mang CuHAp.
* Nhieu xg tia X
Gian dd Xray cua mang CuHAp va HAp dugc the hien trSn Hinh 4. Tii gian do cho thiy mang CuHAp cd cac pic dac trung cho pha ciia HAp ma khdng thay su cd mat ciia cac pha khac.
Hai pic nhieu xa d|c tmng cd ban nhat cua HAp d vi tri gdc nhieu xg 29 « 32" luang iing vdi m^t tinh th^ cd chi sd Miller (211) va d vi tri 26 =; 26° tuang ling vdi mat tinh the cd chi so Miller (002). Ngoai ra, cdn ton lai mgt so cac vach dac trung khac vdi cudng dp nhd han d vi tri 26 w 33.
46, 54° tuong ling vdi cae mat (300), (222) va (004). Ben canh dd ciing xuat hien pic ciia Fe tai 26 = 45" va cua hdn hgp oxit CrO.19FeO.7NiO tai gdc 26 ^ 44" va 51"dac tmng cho nSn TKG316L [14. 15]. Nhu vay, mang CuHAp tdng hgp dugc cd dang tinh th€va dan pha eiia HAp.
1. HAp: I CiaFcaNiO; 3 Fc
J'ff*^.*'''^^'' i^i-,-^v''f»M^*M
10 IS 20 25 30 35 40 4S 9
26(«e)
Htnh 4. Gidn do nhieu xg tia X cUa mang CuHAp vd HAp
Vo Thj Hanh, Ph^m Thi Nam va Dinh Thj Mai Thanh
Tir gian do nhieu xa tia X dnh dugc dudng kinh tinh the CuHAp khoang 35.37 run theo cdng thiic Scherrer (cdng thuc 4) va gia tn khoang each giiia cac mat phang tinh thS (d) tai mat phing (002) va (211), So sanh mlu CuHAp Idng hgp duge vdi HAp theo lieu chuin NIST [16] va mang HAp ban diu cho thiy cac gia ni d tai cac mat phang (hkl) va cac gia tri hing sd mang a, b, c giam (Bang 2). Nguyen nhan do ban kinh ion Cu^* (0.96A) nhd ban ban kinh ciia Ca"" (0,99A) nen khi thay thSCa^*bing Cu^' dIndSn dudng kinh cua linh the CuHAp nhd hon cua HAp.
Bdng 2.'Gid tri khodng cdch giira cdc mat phdng tinh the vd gid tri cdc hdng so mgng cua mdng NoHAp thu duac so vai tieu chuan NIST
Gia tri d(002) (A) d(2in(A) a = b (A) c(A)
HAp |16|
3,44 2,82 9,445 6,88
HAp 3,438 2,815 9,426 6,876
CuHAp 3,437 2,791 9,330 6,874
* Kinh hiin vi dien tuquet (SEM)
Hinh 5. Hinh anh SEM cua mang HAp va mdng CuHAp
Hinh anh SEM cua mang CuHAp va HAp dugc the hien tren hinh 5. Ket qua cho thay sy c6 mat cua Cu da lam thay ddi liinh thai hpc cua mang HAp. Mang HAp ban dau cd dans hinh tam vdi kich thudc tmng binh 150 x 25 nm, mang CuHAp thu dugc khi ion Cu^* tliay the mdi phan ion Ca^* cd tunh dang hinh que va kich thudc tmng binh khoang 102 x 11 nm.
2.2.4. Ddng hoc ciia qua trinh trao doi ion
Tir ket qua nghien eiiu sy bien doi dung lugng trao ddi ion Cu^* theo thdi gian ciia mang HAp cd the tinh dugc ti sd t/Q (bang 3) va xay dung dd thi cua phuang trinh dpng hpc gia bac I theo cdng Ihiic 2 (Hinli 6a) va gia bac 2 theo cdng thirc 3 (Hinh 6b).
Bdng 3. Gid tri ln(QrQd vd t/Q biin ddi theo thdi gian t t (phiit)
Q. (mg/g) ln(0,-Q,) t/Q, (phut.g/rag)
2,5 8,11 1,28 0,31
S 8,75 1,08 0,57
10 9,44 0,81 1,06
20 10,14 0,45 1,97
30 10,96 -0,30 2,74
60 11,58 -2,12 5,18
80 11,70
^-6,30 6,84
Tong hpp vd dgc trung mdng Hydrnxyapaiit pha tgp dongnm nen thep khdng gi 316L
1 0
1^
W -3
-»
-5 -6 -7
'•V /=
\ ? Ff
(a)
•OpOBrTli •1,872
=0,9197
/fi«/r 6. Do thi cua phuang trinh ddng hgc gid bdc I (a) vd gid bdc 2 (b).
Dua vao dd thi ciia phuang trinh ddng hpc ciia qua trinh trao doi ion theo phan ling gia bac 1 va gia bac 2 cdthe xac dinh duge hang sd tdc do cua phan img (k) va dung lugng trao ddi ion d trang thai can bang (Q^). k6l qua chi ra trong Bang 4. Vdi phuang trinh ddng hoc gia bac 1, Q^ tinh theo dd thi cd gia in 6,50 mg/g, gia tri nay khac xa so vdi Q, lir thuc nghiem (11,70 mg/g), dong thdi he sd hdi quy R^ thip (0,9197) Trong khi do theo phuang trinh ddng hpe gia bac 2, Q^ tinli theo dd thi cd gia trj 11.99 mg/g (xap xi vdi Q^ thyc nghiem) va he so hdi quy R- cao (0.9993), chiing td qua trinh trao ddi ion Cu""" vdi mang HAp luan theo phuang trinli ddng hpc phan iing gia bac hai vdi hang sd tdc dd phan ung k = 3,57,10"" g/mg.phui.
Bdng 4. Cdc gid tri k vd Q^tinh theo phuang trinh ddng hgc gid bgc I vd bac 2 Phuvng trinh dong hoc gia bSc m6t
Q.
6,50 kl 8,77.10-'
R' 0,9197
Phu'oiig trinh dong hoc gia bac hai Q. 1 i.1 11,99 1 3,57. IO"'
R' 0,9993
Q.
thu-c nghiem 11,70 2.2.5. Hoat tinh khang khuan
Hinh 7 the hien kel qua kiem tra kha nang khang khuan cua mang HAp va CuHAp vdi chung khuan P.aemgimosa bang phuang phap khuech tan dTa thach. Kel qua cho thay mang HAp (I) khdng CO hieu iing vdi chiing khuan nay cdn mang CuHAp (3) cd hoat linh khang khuan vdi viing uc che dat 3 mm.
Hinh 7. Khd ndng khdng khuan cda mdng HAp (I) vd CuHAp (3)
Vo Thi Hanh, Pham Thj Nam va Dinh Thj Mai Thanh 3. Ket luan
Mang CuHAp da dugc tdng hgp bing phuang phap trao ddi ion giiia mang HAp_ vdi dung dich Cu^*, ndng do Cu^* 0,02 M thich hgp eho qua trinh idng hgp va thai gian tigp xiic tdi uu la 30 phut. Mang CuHAp thu dugc dem pha, tinh the hinh que vdi kich thudc trung binh 102 x 11 nm, Tir ket qua nghien ciiu da md ra trien vpng iing dung mang CuHAp larii vat lieu y sinh va d?c bi^t vdi su cd mat Cu trong mang HAp se lam lang kha nang khang khuan va tang linh tuong thich sinh hpc cho mang.
TAI LIEU THAM KHAO
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Tong hpp vd dac tnmg mdng Hydrox},'apalit pha tap dong tren nen thep khdng gi 3I6L
ABSTRACT
Synthesis and characterization of copper dope hydroxyapattte coating on 316L stainless steel Vo Thi Hanh', Pham Thi Nam" and Dinh Thi Mai Thanh^
Basic Science Faculty. Hanoi University of Mining and Geology 'Institute for Tropical Technology, Vietnam Academy of Science and Technology University of Science and Technology cf Hanoi. Vietnam Academy of Science and Technology
Copper doped hydroxyapalile coatings (CuHAp) were synthesized on 316L stainless steel (316L SS) substrate by the ion exchange method between HAp coalings and Cu^'' solution. The affected factors of Cu^* concentration and immersion time lo the process were studied The results showed that Cu^"^ ions were replaced by a part of Ca^* ions in the structure of HAp lo form CuHAp coating with the atomic ratio Cu/Ca of 0.0124 and the ratio (Cu + Ca)/P of 1.571. The analytical results of morphologies, structure, phase composition showed that the CuHAp obtained coatings have crystal structure, single-phase with rod shapes. In addition, the kinetic of the process was a pseudo-second order model with ion exchange capacity at equilibrium 11.99 mg Cu^Vg HAp and rate constant 3,57 >= 10'" g/mg.niin. CuHAp had an antibacterial activity against a slrain of P.aerugimosa with the inhibitory radius of 3 mm.
Keywords: 316L stainless steel, copper doped hydroxyapalile coalings, ion exchange.
