KHOA HOC CONG NGHE
IXIGHIEIXI CLIU TiCH HOP D A TilXIH KHAIVG BEIXIH BAC LA, RAY IXIALI VAO iXl£lXl Dl TRUYEIXI GIOIXIG LlJA CHAT LUOIXIG B A C THOIVI 7 BAIXIG PHUOIXIG P H A P IVIABC
Nguygn TU Minh Nguy$t\ Nguyen Thi N h ^ ^ Nguyen Bk NgQc\
Nguyen Thi Oanh\ Le TTii NgpcS Dang Van D u y ^ ^ D ^ Van Khdi^
T6MTAT
Benh bac la (BB) va ray nau (BPH) gay ra boi Xanthomonas oryzae pv. oryzae {Xod) va Nilaparvata lugens Stalls.
hat tac nhan gay thiet hai nghiem trpng nhat ddi vol san xuat lua gao. Nghien cihi nay diroc thuc hien nhiim k^t hop hai tinh trang mong muon G^hang bac la va ray nau) vao nen di truyen ciia gidng liia chat luong Bdc Thom 7.
Cac ddng liia khang don tinh trang duoc phat trien bang phuoTig phap MABC, sau dd tiep tuc lai tao, chon loc kieu hinh tinh khang, kieu gen de chgn tao ra cac ddng tich hop da gen khang da tinh trang. Chi thi phan tu lien ket gen khang duoc su dung de chon loc gen muc tieu xa5, Xa7, Bph20vk Bph21 va bo 52 va 56 chi thi phan tu da hinh bd me dinrc sii dung de chgn loc nen di truySn. K^t qua da chgn dune 03 dong Iiia thuan N2.3.38.39, N2.3.55.76 va N2.3.64.18 mang 4 gen khang xa5, Xa7, Bph20\a Bpb21, cd kha nang chdng chiu tdt vdi b^nh bac la va ray nau trong lay nhi6m nhan tao, mang cac dac di^m nong sinh hoc tirong tu giong gdc BSc Thom 7. Ddng liia N2.3.38.39, dupc dat ten la gidng AG 1-4, da duoc khao nghiem tac gia, khao nghiSm san xuat va gui khao nghiem ca ban trong vu xuan nam 2020.
"XxtMHibd:. Benh bac la, tich hpp da tinh khang, da gen khang, MABC, ray nau.
I.DATVANDE
Viec ung dung cong nghe chpn giong nha chi thi phan tif va lai tra lai de h6 trg cho chon giong truydn thong nham cai tien cac giong liia chat luong, gnip tang kha nang chdng chiu voi sau benh hai la huong nghien cmi trpng tam ciia cac chuong trinh chpn tao gidng cay tr6ng noi chung va cay lua noi rieng. Nho su h6 trp ciia cac ky thuat sinh hoc phan tu, thoi gian de cai tien 1 giong liia se duoc nit ngdn va giong liia cai tien vdn giij dupc cac dac tinh nong sinh hpc quy cua giong gdc, dong thoi tich hop duoc nhieu gen khang sau benh hieu qua.
Benh bac la va ray nau la nhimg tac nhan gay hai chinh ddi voi cay lua. Su xuat hien nhung chung vi khuan bac la men va su bi^n doi cua cac biotyp ray nau CO the nhanh chong be gay tinh khang ciia cac gidng Ilia mang gen khang. De quan ly co hieu qua tinh khang sau benh hai chinh, chien luge chpn gidng hien dang duoc cac nha khoa hpc tren toan the gicri tap trung nghien cuu la chii trong vao viec tan dung nhirng gen khang pho rong, tich hop nhieu gen
' Vien Di truySn Nong nghiep Email: [email protected]
" Bg Nong nghiep va PTNT
khang chinh va ca cac QTL vao ciing mot giong (Miah&cs.,2012).
Tir nhijmg ket qua danh gia ciia Vien Di truyen Nong nghiep ve kha nang khang benh bac la va ray nau cua nhimg dong liia chuan mang gen khang do IRRI cung cap, hai dong dSng gen IRBB5 mang gen khang xa5 vk IRBB7 mang gen khang Xa7 cb kha nang khang tot vdi 54 chiing Xanthomonas oryzae pv. oryzae iJCod) gay benh bac la thu thap tai mien Bdc va mien Trung Viet Nam. Nguon gen khang benh ray nau IR71033-121-15 (mang hai gen Bph20, Bph21) do IRRI cung cap qua sang loc tinh khang da the hien kha nang chdng chiu kha tot voi quan the rdy nau tai mien Bdc. Chinh vi vay, trong nghien cim nay, cac gen khang benh bac la xa5. Xa7, gen khang ray nau Bph20, Bph21 dupc chpn la nhung gen khang muc tieu de cai tien gidng Ilia chdt luong Bdc ' n i a m 7 .
2. VAT UEU VA PHUDNG PHAP NGHIBII CUU 2.1. V§t lieu nghien cmi
- Giong Ilia cdn cai tien: Giong liia Bdc Tham 7 (BT7) la gidng liia tham chat lupng duoc trong pho bien b cac tinh mien Bdc. Giong BT7 co thoi gian sinh truong ngdn, nang suat trung binh 50 - 55 ta/ha, ham luong amylose 13,0%, chat lirong gao ngon, hat gao trong, com mem, tham. Tuy nhien, gidng co
37
KHOA HOC CdNG NGHE nhiTOC diem la chiu ret kem. nhitm ray, nhiim benh
dao 6n trung binh. nhiSm bSnh bac la njng. Do vay, giong BT7 duoc lira chon de tich hop da gen khang b t a h bac la, ray nau nham nang cao kha nang chdng chiu ctia giong voi benh bac la va ray nau.
- Cac dong NILs mang gen khang muc Heu va cac giong liia nhiSm chuan do IRRI cung cap bao g^m:
+ IRBB5 (mang gen khang bac la xa5), IRBB7 (mang gen khang bac la JUii), IRBB64 (mang da gen Xa4-i-xa5-i-Xa7i-X!i2D.
+ Dong Ilia IR71033-121-15 mang hai gen khang r4y nau Bph20, Bph21.
+ Gidng IR24 nhiSm chuan ' »'• ^ a Bio"8 ™ ^ nhiim chuan ray nau.
- Bo vat li6u lai tao la cac dong lua the he BC4F, duoc tao ra tir phep lai giOa giong lua chat lupng BT7 va cac dong liia mang da gen khang bac M/riy nau.
• cac chi thi phan tu lien ket gen muc beu (Bang 1).
• Bp chi thi SSR cho da hinh giila cac gidng liia bd me tren 12 NST: 52 chi thi da hinh gitia BT7 va IRBB64; 56 chi thi da hinh giira BT7 va IR71033-121- 15 duoc sir dung de kiem tra nen di truyen cua cac dong vat lieu.
Bang 1. Cac cht thi phSn ttr lifin k^t vdi cic cen khancr muc tieu su dung trone chon loc cA thi Gen
khang xa5 Xa7
Bph20
Bph21 NST
5 6 4 4 12 12
Chi thi lien ket
RM163
P3 MS5 MSIO S12091A RM3331
Khoang each (cM) 5.6 3.4 2.9 0.7 3.8 4.0
Trinh tii moi xuoi/ngirac
F: GCCTCGAGCATCATCATCAG R : ATCAACCTGCACTTGCCTGG F: CAGGAATTGACTGGAGTAGTGGTT R: CATCACGGTCACCGCCATAT F: TTGTGGGTCCTCATCTCCTC R: TGACAACTTGTGCAAGATCAAA F: CAATACGAGAAGCCCCTCAC R CTGAAGGAACACGCGGTAGT F: TGGGGTTAAATGTTGCCTCT R: CATATGTGGGAGCAGACTAGCA F: CCTCCTCCATGAGCTAATGC RAGGAGGAGCGGATTTCTCTC
TLTK
Blair etal., 2003 Porter &CS.. 2003
Rahman & cs..20O9
2.2. Phirong phap nghien a h i 2.2.1. Phuong phap lai tao
I Chgn Igo dfing BT7 mang da gen khang dim tinh (rang |
I BC4F1 I X 1 BC^F, I
D5ngqui v^BpblO, Bpiai liin dl i™>Ai BT7
Tifihv I Mfl
I BC.F;
Dftng ti'ch hgp da gen khang bac la v& ray nau
' Chpn Ipc
• Chpn Ipc
- Chpn d6ni:
- Lay nhiim
• Dinh fiia d$c linh
I XG\-M{i''a5,Xa7,Bph20.Bph2l) \
Hinh 1. Sa d6 chpn t?o giong liia tich h(M) da gen k h ^ b$nh bgc 1^ rdy nSu
Phuong phap chon tao dua vao chi thi phan tu va lai tro lai (MABC) duoc sir dung de chpn tao giong Ilia mang da gen chong chiu voi benh bac la, ray nau.
Cac dong khang don tinh trang duoc phat trien bang phuong phap MABC, sau do tiep tuc lai tao cac dong mang da gen khang don tinh trang voi nhau va sang loc kieu hinh, kieu gen de tao ra cac dong mang da gen khang voi da tinh trang (bac la, ray nau) (Hinh 1).
2.2.2 Phuong phap phan ti'ch kieu gen bing chi thi phan tir
• ADN duac tach chiet tir mhi la non su dung phuong phap tach nhanh phuc vu cho PCR cua Wang
& cs. (1993). Ti^n hanh ky thuat PCR voi t6ng dung dich phan ung la 15 pi bao gom 50 ng ADN tone so 0,15 pM m6i, 0,2 mM dNTPs, 1 X dich dem PCR 2 5 mM MgCla va 0,5 don vi Taq TaKaRa. h\tM kj-' phan ling PCR: 95"C - 7 phiit; 40 chu ky cua: 940c i s giay, SS^C - 30 giay, 72°C - 2 phiit; giu miu a 4«C S'
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 7/2020
KHOA HQC CONG NGHE pham PCR duoc dien di tren gel agarose gel TBE
0,5X 2,5% CO b6 sung edithium bromide de xac dinh su CO mat cua gen khang muc tieu va danh gia n^n di truydn cua cac ca the.
2.2.3. Phuong phap danh gia tinh khang/nhiSm benh bac la, ray nau bang lay nhiim nhan tao
- Lay nhiim nhan tao bdnh bac Is. bang phuong phap su dung keo nhiing vao dung dich vi khuan (nong do 10«-10^ CPU) cat dau la lua d€ tao vet thuong. Thi nghiem lay nhiSm duoc thuc hien sau khi cay liia 45 ngay, m6i cay nhiim 5 la vai gi6ng nhilm chuan la 1R24. Ddi chiing am duoc lam vai nuoc cat khu tning. Chieu dai vet benh dugc do sau 18 ngay lay nhiem. Ket qua danh gia dua tren thang danh gia benh tieu chuan (IRRI, 2013).
- Lay nhiim nhan tao ray nau: Ma 10- 15 ngay tuoi (2-3 la) cua gidng nhiem chuan TNI dupc dung de nuoi ray. Ray tuoi 1-2 duoc tha vao long thi nghiem voi mat do 4- 5 con/cay ma. Thai di^m danh gia duoc tien hanh khi 100% giong nhiim chuan TNI ch^t (khoang 10- 15 ngay sau khi tha ray). Ket qua danh gia dua tren hieu chung gay tdn thuong, thang diem dua tren thang danh gia benh tieu chuan (IRRI, 2013).
2.3. Phuong phip xu ly sd li^u
- Du lieu, so heu dong rugng dupc phan tich, xu ly bang cac phan mem IRRISTAT v. 5.0 va Microsoft Excel 2010.
Phan bich sd lieu kieu gen bang phin mem Graphical genotypes 2.0 (GGT v. 2.0).
IKfTQUAVATMOLUAN
3.1. Dinh gii, sing Ipc ngudn v^t l i ^ k^ thira B ^ Thom 7 tich hpp da gen khing don tinh trying
2 quan the BC4F, N l . l (BT7/IRBB64////BT7) va N1.2 (BT7/IR71033-121-15////BT7) dupc gieo trdng lam nguon vat lieu. Cac chi thi phan tu hen ket chat vdi cac gen dich: RM153 (gen xaSi, P3 {XaT), MSIO {Bph20i, S12091A {Bph21) dupc su dung de xac dinh cac ca the mang gen khang dich trong nguon vat lieu.
Ket qua phan tich kieu gen cua quan th^ N l . l (BT7/IRBB64////BT7) gdm 153 ca the da xac dinh duoc 21 ca the mang ca hai gen khang benh bac li xaSva Xa7b trang thai di hpp tii (Hinh 2). Ket qua phan tich nen di truydn 21 ca the mang 2 gen khing vdi 52 chi thi phan ttr cho da hinh bd me da xac dinh dupc 7 ca the cd nen di truyen gidng voi BT7 (> 98%) (Hinh 3). Cac ca the nay dupc chpn lam vat lieu cho thi nghiem tich hpp da gen khang hai tinh trang (bac la, ray nau).
Hinh 2. K^t qua phan tich ki^u gen q u ^ th^ N l . l v6i chi thi RM153. P3 lien k^t gen khing xa5, Xa?
\ \
Hinh 3. K^t qua phin tich n^n di truy^ cua c i th^ sd 19 q u ^ th^ Nl.l b ^ phdn m^m GGT v. 2.0 Ghi chu: Ban dd vi tri cua 52 chi thi SSR tien 12 NST cua ca the sd 19. Phan bieu thi mdu do la nen di tmyen BT 7, phan xanh la di hop tir.
Thi nghiem phan tich kieu gen tiep theo dupc 15////BT7) gdm 125 ca th^ da xac dinh dupc 22 ca thuc hien tren q u ^ the N1.2 (BT7/1R71033-121- t^^ mang ca hai gen khang riy nau 5p/?il9va ifpAi'^
KHOA HOC CONG NGHJ (Hinh 4). Ket qua phan tich nen di truyen cua 22 ca
the nay vol 56 chi thi cho da hinh bd me da chpn Ipc dupc cac ca the sd 4, 9, 16 va 19 co nen di huyen
MSIO
gidng vdi BT7 (> 98%). Cac ca the nay dupc chon lam vat heu tao quan the tich hpp da gen khang hai tinh trang.
Hinh 4. K^t qua phin tich k i ^ gen quM m^ N1.2 vdi chi thi MSIO, S12091A Hen kgt gen khing/?cift^^
3.2. Lai tao cic ddng Bac Thorn 7 tich hpp da gen khing hai tinh tr?uig bac l i v i r4y niu
Cac ca the dupc xac dinh mang hai gen khang voi mot tinh trang (bac la/ray nau) va mang nen di fruyen > 98% ciia gidng goc BT7 cua cac quan the N l . l , N1.2 dupc su dung lam vat Heu lai tao tich hop da gen khang hai tinh trang. Kdt qua vu xuan 2018 da lai tao duoc qudn the N2.3 (BT7/IRBB64////BT7)/(BT7/IR71033-121-
1 5 / / / / B T 7 ) gom 69 ca thd.
K^t qua phan tich kieu gen cac ca the cua quan the N2.3 da xac dinh dupc 15 c i thd mang ca bdn gen k h i n g xa5, Xa7, Bph20vk Bph21 b trang thai di hpp hi (Hinh 5).
Hinh 5. K^t qui phin tich ki^u gen q u ^ thd N2.3 vdi cac chi thi Hen kdt gen khing muc tidu (vu xuin 2018)
Cac c i thd mang 4 gen khang tiep tuc dupc theo doi cac dac diem ndng sinh hpc, cic ydu td cau thanh nang suat, chat luong va dupc sii dung de phat trien thanh nhirng ddng mang gen khang benh hac la va ray nau ddng thoi giii dupc cic dac tinh ndng sinh hpc gidng vdi gidng gdc BT7.
3.3. Dinh g i i ki& hmh tinh khing, cic d$c didm nOng sinh hpc, cic y^u td c^u thinh nang su^t, kha ning khing siu b ^ h-fin ddng ruOng cua quan th^
tich hpp da gen khing hai tioh trang (vu miia 2018) Quan thd thd he BC4F2 tich hop da gen khang hai tinh trang bac la, ray nau duoc gieo trdng va dinh gii kieu hinh tinh khang va cac dac diem nong sinh hpc, nang suat, kha nang khing sau benh tren dong ruong trong vu miia 2018.
Ket qua dinh gii da thu dupc 15 cay diu ddng mang cac dac tinh nong sinh hpc gan voi giong gdc BT7, bieu hien khang vdi benh bac la. Cac cay diu ddng dupc thu hat theo ca the, gieo trong de dua vao thi nghiem thii ray vi ket qua cho thiy ba dong bieu hien chdng chiu kha vai ray niu la dong N2.3.38, N2.3.55 va N2.3.64; 12 ddng cdn lai bieu hien nhidm vira vdi rSy nau. Ba ddng N2.3.38, N2.3.55 va N2.3.64 dupc tiep tuc phit trien thanh ddng tich hpp da gen khang d cic vu tiep theo (Hinh 6, bang 2, bang 3).
Hinh 6. Thi nghiem lay nhiim nhin tao cic chung vi khuan bac la frdn qudn th« tich hpp da gen khing bjic la
rdyniu '
(a): Uy nhiim nhan tao; (b) Ruong tiii nghiem sau 7 ngay lay nhiim
KHOA HOC C O N G NGHE
Bing 2. Kft qui theo dOi mOt s6 djc di^m ndng sinh hgc cua cac c4 W diu ddng tich hgp da gen khing hai tinh trang b j c l i v i ray niu (vu mvia, 2018)
Dong
BT7 (B/c) So bOng/
khom
5.6
Chieu cao cay (cm)
Chieu dai bong (cm)
26.1
So hat chac/bong
Tong so hat
KL 1000 hat(g) 19.3 19.0 18.6
NSLT (g/khom)
17,0 14.3 Bang 3. Mtic di^m v i cip h?i cua cic c i th^ dau d6ng tich hgp da gen khing hai tinh trsuig biic l i v i riy niu
(vu miia, 2018)
snr
1 2 3 4 5
Masd
N2.3.38 N2.3.55 N2.3.64 IR24 TNI
Miic do chong chiu Bacla
Chi^u dai vet benh (cm)
1,5 2.5 3.0 20.6
B i t e 1 1 1 9
Tinh khang Khang Khang Khang Nhiim nang
I^y nau Diem
3.7 3.5 3.8 9
Tinh khang Khang Khang Khang Nhi^m nang 3.4. Kdt qua dinh g i i kidu hmh tinh khing, cic
die didm ndng sinh hpc, c i c ydu td c^u thinh ning su^t, kha ning khing sau benh trdn ddng mdng cua q u ^ thd tich hpp da gen khing hai tinh trgng (vy xuin 2019)
Trong vu xuan 2019, 3 ddng Ida mang 4 gen khing vdi hai tinh trang bac la va ray nau (N2.3.38,
N2.3.55, N2.3.64) duoc gieo trdng va danh g i i kieu hinh tinh khang va cac dac diem nong smh hpc, nang suat va kha nang khang sau benh tren ddng ruong.
Ket qua phan tich kieu gen da xic dinh dupc 18 ca the mang 4 gen khang xa.5, Xa7, Bph20\z Bph21 b trang thai ddng hop tu.
Hinh 7. Kdt qua phin tich kidu gen qu4n thd tich hpp da gen khing hdnh bac li, riy niu vdi cic chi thi lien ket gen khing muc tidu (vu xuin 2019)
Ket qua danh gia trdn ddng ruong cho thay, trong ciing didu kien, cac ddng mang cac dac diem sinh tnrdng, phat trien va hinh thai tuong tu voi gidng gdc BT7. Tong hpp ket qua theo doi va danh gia trong vu xuin 2019 da thu dupc 3 ddng BCjFa
Bing 4. Tdng hpp kdt qua dinh gii d^c didm n6ng sinh hpc v i nang suat cua cic ddng liia tich hpp da gen khing hai tinh trang cd tridn vpng (vu xuin, 2019)
N2.3.38.39, N2.3.55.76 va N2.3.64.18 tich hpp 4 gen khang xa5, Xa7, Bph20, Bph21, bieu hien tinh khing vdi bac la va ray nau de tiep tuc phat trien ddng thuan (Bang 4, Bang 5).
TT 1 2 3
Ma sd dong N2.3.38.39 N2.3.55.76 N2.3.64.18 BT7 (S/c)
TGST (ngay) 120 118 120 119
Chieu cao cay (cm) 108.5 108.9 107.5 108.4
So bong /khom
7 7 6 6
Dai bong (cm) 25.82 24.84 24.24 25.2
Sd hat chic/bdng
156.8 141,6 159.6 160.5
Tdng sd hat 172.5 169,9 183,5 184,6
KL 1000 hat(g)
18,5 18,1 18,8 18.0
NSLT (g/khdm)
20,3 17,9 18,0 17,3
KHOA HOC CdNG NGHt
Bing 5. T6ng hgp kft qui dinh g i i ki«u gen v i \dia hinh tinh khing ciia cic ddng liia tich hgp da gen khing
TT
1 2 3
Ma sd d6ng
N2.3.38.39 N2.3.55.76 N2.3.64.18 BT7 (B/c)
Danh ?ia kien pen Bacla
xa5 x x
X
Xa7 x
X X
Ray nau Bph20
X X X
Bph21
X X X
Bacla CDVB
1.9 2,2 2,9 19.8
Diem 1 1 1 9
Tinh
Nhi^m
R^y n^u Di^m
3,8 3,7 3,7 9
Tinh khang Khang Khang Khang Nhi^m Chii thich: CDVB: chieu dai vet benh
3.5. Gieo tr^ng, chgn ddng thuin mang da gen ' * " ^ "*" ''"^c gieo trdng tren ddng ruong va danh khing da tinh trjmg (vu m i a 2019) 8"* ki^u gen khang. Ket qua danh gia kieu gen cho Trong vu mua 2019. 3 ddng Ilia N2.3.38.39. ft/y.'^f 3 ddng ddu mang cic gen khang bac lava riy N2.3.55.76 va N2.3.64.18 mang da gen khang bac la " " " ' " ' ^ « ^ * ° " 8 hop Ur (Hmh 8).
Hinh 8. I^t qua phin tich kidu gen cic ddng tridn vpng tich hpp da gen khing b^nh b^c Ii, riy niu vdi cic chi thi lien kdt gen khing muc tidu (vy mia 2019)
Thi nghiem dinh gia kieu hinh tinh khang dupc *^li^"g chiu viia vdi ray niu (diem 34) hong dieu thuc hien frong vu miia 2019 cho thay ca ba ddng ^ ^ " ^^y nhidm nhin tao (Bang 6,7).
deu CO kha ning chdng chiu tdt vdi bac li (diem 1),
Bing 6. Tdng hpp kdt qua dinh g i i kidu gen v i kidu hinh tinh khing cua cic ddng lua tich hpp da gen khing hai tinh hang c6 tridn vpng (vy m(ia, 2019)
TT 1 2 3
Ms s6 d6ng N2.3.38.39 N2.3.55.76 N2,3.64.18 BT7ff)C)
Danh gia ki^u gen khang Bacla
xa5
X X X
Xa?
X X X
Ray nau Bph20
X X X
Bph21
X X X
Danh gia kieu hinh tinh khang Bacla
CDVB (cm)
2,5 3,0 3,5 21,9
Dilm 1 1 1 9
Tinh khang Khang Khang Khang Nhi^m
Ray nau Diem
3,7 3,8 3,8 9
Tinh khang Khang Khang Khang Nhi^m Chtj thich: CDVB: chieu dai vet benh
Bang 7. Tdng hop k^t qui dinh gii die didm ndng sinh hpc v i ning suit ciia cic ddng liia tich hgp da gen khing hai tinh trang cd tridn vpng (vii miia, 2019)
TT Ma sd ddng
N2.3.38.39 N2.3.55.76 N2.3.64.18 BT7(BC)
TGST (ngay)
Chieu cao cay (cm) 115.5 118.5 113.5 117,5
Sd bong /khdm (cm)
25,8 25,5
Sdhat c h i c / bong 185,4
KL 1000 hat(g)
18.3
CV%
NSTT (ta/ha) 56,7 54,5 52,5 48,2
42 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG
KHOA HOC CdNG NGHE Tir ket qua theo doi cac dac didm nong sinh hpc,
phan tich kieu gen va danh gii kieu hinh tinh khang benh bac la va ray nau bang lay nhidm nhan tao trong vu miia 2019 da chpn duoc 03 ddng lua N2.3.38.39. N2.3.55.76 va N2.3.64.18 mang 4 gen khing xa5, Xa7, Bph20vk Bph21, co kha ning khang tdt vdi benh bac l i va chdng chiu kha vdi ray nau trong liy nhidm nhin tao, mang cac dac diem nong sinh hpc tuong tu gidng gdc Bic Tham 7, Dong lua N2.3.38.39, duac dgt ten l i gidng AGl-4 va dupc gui khao nghiem co ban trong vu xuan nam 2020.
Hinh 9. Gidng Ida tridn vpng AGI-4 trong hd thdng khao nghidm qudc gia, vu xuin 2020
4.KErLU0N
- Da nghien cuu tich hpp thanh cdng 2 gen khang benh bac l i xa5, Aa7vi 2 gen khang ray nau Bph20, Bph21 vao gidng lua chat luong Bic Thom 7 bang phuong phip chpn gidng dua vao chi thi phan tir va lai trd lai (MABC).
- Ket qua lai tao, chpn Ipc kieu gen, danh gia kieu hinh da chpn dupc 03 dong liia tich hpp da tinh khing N2.3.38.39, N2.3.55.76 va N2.3.64.18 mang 4 gen khang xa5, Xa7, Bph20vk Bph21 cb kha nang khang tdt vol benh bac la va chdng chiu kha voi riy nau trong lay nhidm nhan tao, mang cac dac diem nong sinh hpc tuong tu gidng gdc Bic Thom 7.
Gidng lua AGl-4 (duoc phat frien tir ddng N2.3.38.39) mang ndn di truydn cua gidng lua chit
lupng Bic Tham 7, mang 4 gen khang xa5, Xa7, Bph20 v i Bph21, cd kha ning khing benh bac la (diem 1) v i khing vua voi ray niu (diem 34) trong lay nhiem nhin tao. Ket qua theo doi cic die diem nong sinh hpc cho th^y gidng lua AGI-4 cd cac die diem nong sinh hpc tuong tu gidng gdc Bic Thom 7, ning suat thuc thu cao hon ddi chung Bic Tham 7 khoang 17%. Gidng lua AGI-4 da dupc khao nghiem tac gia, khao nghiem sin xuat va gtri khio nghiem ca ban tirvu xuan 2020.
LOI CAM ON
Cong trinh nghien cdu la kdt qua nghien cdu cua di tai cap Nha nude "Nghien cdu ti'ch bop da gen khang benh bac la, ray nau va dao on cd bdng vao gidng lua chat luong bdng chi thi phan tii (MABC) "
thuoc Chmmg trinh Cdng nghe Sinh hoc Nong nghiep.
TAI UEU THAM KHAO
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{Oryza sativa L.) Theor Appl Genet, 107: 62-73.
2. Intemational Rice Research Institute (IRRI) (2013). Standard Evaluation System (SES) for Rice, 5th Edition, 2013.
3. Porter BW, Chittoor JM, Yano M, Sasaki T, &
White F F (2003). Development and mapping linked to the nee bacterial bUght resistance gene Xa7. Crop Science, 43:1484-149.
4. Rahman M.L, W. Jiang, S. H. Chu, Y. Qiao, T.
H. Ham, M. 0 . Woo, J. Lee, M. S. Khanam, J. H.
Chin, J. U. Jeung, D. S. Brar, K. K. Jena, H. J. Koh (2009). High-resolution mapping of two nee brown planthopper resistance genes, Bph20 (t) and Bph21(t), originating from Oryza mmuta. Theor ^ p l Genet, 119:1237-1246.
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NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 7/2020 43
KHOA HOC CONG N G H |
MULTTTRArr INTROGRESSION FOR RESISTANCE TO BACTERIAL BUGHT, BROWN PLANTHOPPER IN THE GENETIC BACKGROUND OF QUALTIY RICE VARIETY, BAC
THOM 7 BY USING MARKER ASSISTED BACKROSSING (MABC)
Nguyen Th; Minh Nguyet, Nguyen Thj Nhai, Nguyen Ba Ngoc, Nguyen Tlii Oanh, Le Thi Ngoc, Dang Van Duyen, Dao Van Khoi Summary
Bacterial Blight (BB) and Brown Planthopper (BPH) caused by Xantiiomonas oryzae pv. oryzae {Xod) and Nilaparvata lugens Stil, respectively, are the most destructive disease and msect causing significant reduction in nee production. The marker-assisted baclc crossing (MABC) technique is typically used to improve disease and insect resistance for nee. The use of broad-spectrum resistance genes, mtegrating major resistance genes into quality rice varieties, is a strategy to prevent or delay tlie breakdown of resistance of rice cultivars. In this study, multitrait introgression is attempted to combine two desirable traits CBB and BPH resistance) into a genetic background of quality rice variety, Bac Thom 7. The single- trait resistant hues were developed by MABC method, and the crossing among single-trait resistant lines to obtain multitrait introgression lines which earned multiple resistance genes. In each stage of backcrossing, foreground selection was carried out for the target resistance genes (xa5, Xa7, Bph20 snd Bpb21), usmg linked markers, while background selection was done usmg a set of 52 and 56 parental polymorphic SSR markers respectively. The results have been obtained 03 elite lines, N2.3.38.39, N2.3.55.76 and N2.3.64.18, carried 4 resistance genes xa5, Xa7, Bph20anA Bph21, which shown resistance to BB and BPH in artificial infection, and earned the agro-biological characteristics similar to the onginal cultivar, BT7. Line N2.3.38.39, named AGI4, was conducted for author tests, production trials and sent for naUonal basic testing in the spring crop of 2020.
KeyvioT^'sr. Bacterial blight, multitrait intiogression, multiple resistance genes, MABC, brown planthopper.
Ngudi phin bidn: PGS.TS. Li Tu& Nghia Ngiy nhin bai: 21/5/2020
Ngiy thdng qua phan bidn: 22/6/2020 Ngiy duyet ding: 29/6/2020
