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Fig. 1. Fractionation of the 26S proteasome from culturedrice (gation analysis. Samples (12 mg protein) obtained by BiogelA-1.5m column chromatography were analyzed according tothe procedure described in the text
Fig. 2. Electrophoretic analysis of degradation of poly-Ub-125times in the presence of 10 mM MgI-lysozyme conjugates by the purified 26S proteasome
Fig. 4. Effect of pH on three peptidase activities. The degra-dation activities by rice (A) and spinach (B) 26S proteasomesfor the indicated substrates were measured at various pHvalues in the absence of SDS
Fig. 5. Degradation of rat ODC by purified 26S proteasomesfrom rice, spinach and rat. The ATP- and antizyme-depen-proteasomes (1dent degradation of35S-labeled ODC with purified 26S �g) was assayed by measuring the conversioninto TCA-soluble fraction as described in Section 2.

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