ناﺮﯾا ﯽﺳﺎﻨﺷ ﺖﺴﯾز ﯽﻠﻠﻤﻟا ﻦﯿﺑ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﺠﻨﭘ و يﺮﺳاﺮﺳ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﻫﺪﻔﻫ
ﺪﯿﺳا راﺪﻘﻣ ﻦﯿﯿﻌﺗ و ﯽﯾﺎﺳﺎﻨﺷ يﺎﻫ
بﺮﭼ ﻒﻠﺘﺨﻣ يﺎﻫ ماﺪﻧا ﻦﻏور رد ﻪﻧﻮﭘ ﻪﻧﻮﮔ ود
ﺎﺳ ي ناﺮﯾا
ﺪﺠﻣ يرﺪﺻ ﺐﻨﯾز
1
نﺎﯿﺒﺟر ﻪﺒﯿﻃ ،*
راﺮﺳا اﺮﻫز ،2
ﯽﻧﺎﺑﺮﻬﻣ اﺮﺘﯿﻣ ،3 4
1 - ﺶﻧاد ناﺮﯾا ،ناﺮﻬﺗ ،ﺪﻫﺎﺷ هﺎﮕﺸﻧاد ،ﯽﺳﺎﻨﺷ ﺖﺴﯾز هوﺮﮔ ﻪﺘﺧﻮﻣآ
2 - ناﺮﯾا ،ناﺮﻬﺗ ،ﺪﻫﺎﺷ هﺎﮕﺸﻧاد ،ﯽﺳﺎﻨﺷ ﺖﺴﯾز هوﺮﮔ
3 - ،ﯽﺳﺎﻨﺷ ﺖﺴﯾز هوﺮﮔ نﺎﻣﺮﮐ ﺮﻨﻫﺎﺑ ﺪﯿﻬﺷ هﺎﮕﺸﻧاد
، ناﺮﯾا ،نﺎﻣﺮﮐ
4 - نﺎﻣﺮﮐ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
، ﯽﺘﻨﺳ ﺐﻃ و ﯽﯾوراد نﺎﻫﺎﯿﮔ تﺎﻘﯿﻘﺤﺗ ﺰﮐﺮﻣ ناﺮﯾا ،نﺎﻣﺮﮐ ،
[email protected] mail:
- E
هﺪﯿﮑﭼ
ﺎﺳ ﻪﻧﻮﭘ ﺲﻨﺟ نﺎﻫﺎﯿﮔ )
Nepeta
(
نﺎﯿﻋﺎﻨﻌﻧ هﺮﯿﺗ يﺎﻀﻋا ﻦﯾﺮﺘﮔرﺰﺑ زا ﯽﮑﯾ
، ﻞﯿﻟﺪﺑ صاﻮﺧ عﻮﻨﺘﻣ ﯽـﯾوراد هراﻮـﻤﻫ ﻌﯿـﺳو دﺮﺑرﺎـﮐ ﯽ
رد
ﺐﻃ ﯽﺘﻨﺳ ا ﻪﺘﺷاد ﺪﻧ . ﻦﯿﺘﺴﺨﻧ ياﺮﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد رﺎﺑ
ﻪـﻧﻮﮔ ود ﻒـﻠﺘﺨﻣ يﺎـﻫ ماﺪﻧا ﻦﻏور بﺮﭼ ﺪﯿﺳا ياﺰﺟا و اﻮﺘﺤﻣ يﺎـﺳ ﻪـﻧﻮﭘ
گﺮــﺑ ) راد
N. bracteataﯽﻧﺎــﻫﺎﻣ يﺎــﺳ ﻪــﻧﻮﭘ و (
)
N. mahanensisناﺮــﯾا (
ﺖــﻓﺮﮔ راﺮــﻗ ﯽــﺳرﺮﺑ درﻮــﻣ .
زا ﺎــﻫ ﻪــﻧﻮﻤﻧ
نآ ﻦﻏور و ﺪﻧﺪﺷ يروآ ﻊﻤﺟ ﯽﻌﯿﺒﻃ يﺎﻫ هﺎﮕﺘﺴﯾز يﺎﻫﺪﯿﺳا .ﺪﺷ جاﺮﺨﺘﺳا ناﺰﮕﻫ ﺎﺑ و ﻪﻠﺴﮐﻮﺳ هﺎﮕﺘﺳد رد ﺎﻫ
ﯽﻧﻮﺑﺎـﺻ ﺎـﺑ دازآ بﺮـﭼ
) يزﺎﮔ ﯽﻓاﺮﮔﻮﺗﺎﻣﺮﮐ يﺎﻫ شور ﺎﺑ نﺪﺷ ﯽﻠﯿﺘﻣ زا ﺲﭘ ﺎﻫ نآ راﺪﻘﻣ و ﺪﻧﺪﺷ ﻪﯿﻬﺗ ﻦﻏور يﺎﻫ ﻪﻧﻮﻤﻧ نﺪﺷ يزﺎـﮔ ﯽﻓاﺮﮔﻮﺗﺎﻣﺮﮐ و (
GC-
) ﯽﻣﺮﺟ ﯽﺠﻨﺳ ﻒﯿﻃ
GC-MS.ﺪﺷ ﺶﺠﻨﺳ ( ياﻮﺘﺤﻣ ﻦﯾﺮﺘﺸﯿﺑ
) ﻦﻏور 27 (ﮏﺸﺧ نزو % ياﺮﺑ
ﯽﻧﺎﻫﺎﻣ يﺎﺳ ﻪﻧﻮﭘ رﺬﺑ ﺪـﺷ شراﺰـﮔ
.
ﺎﻫ ماﺪﻧا ﺐﻠﻏا ﻦﻏور رد ي
اﻮﺘﺤﻣ ﻪﻧﻮﮔ ود ي
ﺎﻫﺪﯿﺳا ي ﺎﮕﻣا بﺮﭼ -
3 ﺎﮕﻣا زاﺮﺘﺸﯿﺑ -
6 دﻮﺑ و ، ﻦﯾﺮﺘـﺸﯿﺑ راﺪـﻘﻣ
ﺎﻫﺪﯿـﺳا ي ﺎـﮕﻣا بﺮـﭼ -
3
رد راد گﺮﺑ يﺎﺳ ﻪﻧﻮﭘ رﺬﺑ )
12 / 67 .ﺪﻣآ ﺖﺳﺪﺑ (ﻞﮐ ﻦﻏور % ﺎـﮕﻣا بﺮـﭼ يﺎـﻫ ﺪﯿـﺳا ﺪـﺻرد ﻦﯾﺮﺘـﺸﯿﺑ
- 6 ﺰـﯿﻧ ﻪﻗﺎـﺳ رد ﺐـﯿﺗﺮﺗ ﻪـﺑ
) 05 / 21 (%
رﺬﺑ ، ) 19 / 19 (%
گﺮﺑ و ) 93 / 15 (%
.ﺪﺷ دروآﺮﺑ ﯽﻧﺎﻫﺎﻣ يﺎﺳ ﻪﻧﻮﭘ ﺎﻔﻟآراﺪﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ
- ) ﺪﯿﺳا ﮏﯿﻨﻟﻮﻨﯿﻟ ﺎـﮕﻣا
- 3 ﮏـﯿﺌﻟﻮﻨﯿﻟ و (
) ﺪﯿﺳا ﺎﮕﻣا - 6 (،
ﺎﺑ ﺐﯿﺗﺮﺗ ﻪﺑ 94
/ 13 و % 02 /5 رد ماﺪﻧا ﮏﺸﺧ نزو % ﯽﻧﺎﻫﺎﻣ يﺎﺳ ﻪﻧﻮﭘ يﺎﻫرﺬﺑ
.ﺪﺷ شراﺰﮔ
يﺪﯿﻠﮐ تﺎﻤﻠﮐ :
گﺮﺑ يﺎﺳ ﻪﻧﻮﭘ ،ﯽﻧﺎﻫﺎﻣ يﺎﺳ ﻪﻧﻮﭘ راد
، ﺎﻫﺪﯿﺳا ي ﺎﮕﻣا بﺮﭼ -
3،
بﺮﭼ يﺎﻫ ﺪﯿﺳا ﺎﮕﻣا
- 6،
يزﺎﮔ ﯽﻓاﺮﮔﻮﺗﺎﻣﺮﮐ
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ناﺮﯾا ﯽﺳﺎﻨﺷ ﺖﺴﯾز ﯽﻠﻠﻤﻟا ﻦﯿﺑ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﺠﻨﭘ و يﺮﺳاﺮﺳ ﺲﻧاﺮﻔﻨﮐ ﻦﯿﻤﻫﺪﻔﻫ
Characterization and Determination of Fatty acids in Oils of Different Organs from two Iranian Nepeta species
Z. Sadry Majd1*, T. Radjabian1, Z. Asrar2, M. Mehrabani3 1-Department of Biology, Shahed University, Tehran, Iran 2-Department of Biology, Shahid Bahonar University, Kerman, Iran
3- Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran [email protected]
mail:
- E
Abstract
The genus Nepeta, belongs to Lamiaceae family has been widely used in traditional medicines for their numerous medicinal properties. In this study for the first time, oil content and fatty acid composition of different organs from two Iranian Nepeta species (N. mahanensis and N. bracteata) was characterized and determined. The air-dried plant materials were ground and used for oil extraction with a Soxhlet apparatus and n-hexane as solvent. Free fatty acids (FFAs) were prepared by the saponification of oil samples. FFAs were methylated and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Among the studied organs, the highest total oil content (27% dry weight) was obtained for seeds of N. mahanensis. In most of the studied organs, the percentages of omega 3- fatty acids in the oils were higher than omega 6- fatty acids. The highest content of omega 3- fatty acids (67.12% total oil) was obtained in seed oil of N. bracteata. The highest percentages of omega 6- fatty acids were found in the oils of stems (21.05%), seeds (19.19%) and leaves (15.93%) of N. mahanensis. The highest contents of Alpha- linolenic acid and linoleic acid were reported as 13.94% and 5.02% dry weight for seed oils of N.
mahanensis, respectively.
Key words: Nepeta mahanensis, Nepeta bracteata, omega 3- fatty acids, omega 6- fatty acids, GC
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