ﺘﺳا شور دﺮﺑرﺎﮐ ﻧاﺮﺤﺑ نودﺎﻣ بآ جاﺮﺨ
ﯽ ﮔ هرﺎﺼﻋ رد ﯿﺮ
ي ﺳرﺮﺑ و رﺪﻨﮐ ﻎﻤﺻ زا ﯽ
ﻠﺑﺎﻗ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ
ﻣﺪﺿ و ﯿ ﺑوﺮﮑ ﯽ هرﺎﺼﻋ ﺎﻫ
ﭽﻠﮔ ﺮﺤﺳ ﯿ
1ﻦ ﺟﺎﺣ هرﺎﻬﺑ ، ﯽ
ﻮﻠﻤﺘﺳر
1
د هﺮﻫز ،*
ﯾ
1راﺪ ﻇو ﻦﺴﺤﻣ ، ﯿ
ﺖﺳود ﻪﻔ ﻀﺗﺮﻣ ،1
ﯽ ﺪﻤﺤﻣ
2ي
1 - ﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ﯾ
اﺬﻏ ﻊ ﯾ ﯽ ﻧ ﺪﺣاو ، ﯿ ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،رﻮﺑﺎﺸ ﯽ
ﻧ ، ﯿ ا ،رﻮﺑﺎﺸ ﯾ ناﺮ .
2 - روآﺮﻓ هوﺮﮔ ي
اﺬﻏ داﻮﻣ ﯾ ﯽ ﺸﻫوﮋﭘ ﻪﺴﺳﻮﻣ ، ﯽ
ﺎﻨﺻ و مﻮﻠﻋ ﯾ
اﺬﻏ ﻊ ﯾ ﯽ ا ،ﺪﻬﺸﻣ ، ﯾ ناﺮ .
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 27 / 04 / 1401
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 26 / 07 / 1401
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
ﻧاﺮﺤﺑ نودﺎﻣ بآ ﯽ
،
ژﺮﭙﺳآ ﯾ
،سووﻼﻓ سﻮﻠ ،رﺪﻨﮐ ﻎﻤﺻ
دار ترﺪﻗ ﯾ ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ ،
ﺳﺎﺑﻮﺘﮐﻻ ﯿ سﻮﻠ .
DOI: 10.22034/FSCT.19.130.143 DOR: 20.1001.1.20088787.1401.19.130.13.2
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ : [email protected]
ﻧاﺮﺤﺑ نودﺎﻣ بآ شور ﻪﺑ جاﺮﺨﺘﺳا ﯽ
ﻪﺑ ، ﺷور ناﻮﻨﻋ ﯽ
دﺎﺼﺘﻗا ،ﺪﻣآرﺎﮐ ي
ﺳود و ﺖ ﺤﻣ راد ﯿ ز ﻂ ﯾ ،ﺖﺴ
ﻪﺑ ﮐﺮﺗ جاﺮﺨﺘﺳا رﻮﻈﻨﻣ ﯿ
ﺳرﺮﺑ درﻮﻣ رﺪﻨﮐ ﻎﻤﺻ هﺮﺛﻮﻣ تﺎﺒ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
اﺮﺑ ي ا ﯾ بآ زا رﻮﻈﻨﻣ ﻦ
ﻧاﺮﺤﺑ نودﺎﻣ ﯽ
ﺎﻫﺎﻣد رد ي 120 ﺎﺗ 160 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز تﺪﻣ و داﺮﮔ 5
و 15 ﻗد ﯿ و هدﺎﻔﺘﺳا ﻪﻘ
ﮐﺮﺗ راﺪﻘﻣ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ دار ترﺪﻗ ،ﻞﮐ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ ﺻﺎﺧ و ﯿ ﻣﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ هرﺎﺼﻋ ﺎﻫ ي ﻞﺻﺎﺣ
ﺳرﺮﺑ درﻮﻣ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
ﻪﺑ هرﺎﺼﻋ ﺮﺘﻬﺑ ﺎﺑ هﺪﻣآ ﺖﺳد ﯾ
ﺻﻮﺼﺧ ﻦ ﯿ
ﺸﺷﺎﭘ ﻦﮐ ﮏﺸﺧ شور ﻪﺑ ،تﺎ ﯽ
ﺻﻮﺼﺧ و ﺪﺷ ﻪﻟﻮﺴﭙﮑﻧا ﯿ
زﻮﺗ ﻞﻣﺎﺷ نآ تﺎ ﯾ
ﺻﺎﺧ و تارذ هزاﺪﻧا ﻊ ﯿ
ژﺮﭙﺳآﺪﺿ ﺖ ﯾ
ﺳﻮﻠ ﯽ درﻮﻣ نآ
ﺳرﺮﺑ ﯽ ﺖﻓﺮﮔ راﺮﻗ .
ﺎﺘﻧ ﯾ ﻧاﺮﺤﺑ نودﺎﻣ بآ جاﺮﺨﺘﺳا شور ﻪﮐ داد نﺎﺸﻧ ﺞ ﯽ
ﺛﺎﺗ ، ﯿ ﻨﻌﻣ ﺮ ﯽ راد ي رد
اﺰﻓا ﯾ ر ﺶ ﺖﺳا ﻪﺘﺷاد رﺪﻨﮐ ﻎﻤﺻ هرﺎﺼﻋ جاﺮﺨﺘﺳا نﺎﻣﺪﻧا .ﺑﯿ
ﺮﺘﺸ ﯾ ﮐﺮﺗ راﺪﻘﻣ ﻦ ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ جاﺮﺨﺘﺳا
ﻧاﺮﺤﺑ نودﺎﻣ بآ شور رد هﺪﺷ ﯽ
ﺎﻣد رد ي 160 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز و داﺮﮔ 15
ﻗد ﯿ ﻪﮐ ﺪﺷ هﺪﻫﺎﺸﻣ ﻪﻘ
نآ راﺪﻘﻣ 83
/ 130 ﻣ ﯿﻠ ﯽ ﻟﺎﮔ مﺮﮔ ﯿ ﺳا ﮏ ﯿ رد ﺪ 100 دﻮﺑ ﮏﺸﺧ هدﺎﻣ مﺮﮔ .
دار ترﺪﻗ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ
هرﺎﺼﻋ ﺎﻫ ي ﻞﺻﺎﺣ ﻧاﺮﺤﺑ نودﺎﻣ بآ شور زا ﯽ
ﺘﻧآ ﺎﺑ ﻪﺑﺎﺸﻣ ﯽ
ﺴﮐا ﯿ ﺒﻃ ناﺪ ﯿﻌ ﯽ ﺳا ﯿ ﺑرﻮﮑﺳآ ﺪ ﯿ
دﻮﺑ ﮏ
ﺘﻨﺳ شور زا ﻞﺻﺎﺣ هرﺎﺼﻋ و ﯽ
ﺧ ﯿ ارﺎﮐ نﺪﻧﺎﺴ ﯾ
ﯽ ﺮﺘﻤﮐ ي دار بﺬﺟ رد ﯾ
لﺎﮑ ﺎﻫ ي دازآ DPPH
ﺖﺷاد . ﺎﺘﻧ ﯾ ﻣ ﺞ ﯿ ﺑوﺮﮑ ﯽ ﺛﺎﺗ ﻪﮐ داد نﺎﺸﻧ ﯿﺮ
هرﺎﺼﻋ ﺎﻫ ي ﻞﺻﺎﺣ زا شور ﺎﻫ ي ﺧ ﯿ بآ و نﺪﻧﺎﺴ نودﺎﻣ
ﻧاﺮﺤﺑ ﯽ
، ﺮﺑ ﺮﺘﮐﺎﺑ ي ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ
،سﻮﺋروا ﺑﯿ ﺮﺘﺸ زا ﺮﺘﮐﺎﺑ ي اﯾ ﺷﺮﺸ ﯿ ﻠﮐﺎ ﯽ هدﻮﺑ ﺖﺳا . ردﻮﭘ
رﯾ ﻧﺎﺷﻮﭘﺰ ﯽ ﺖﻈﻠﻏ رد رﺪﻨﮐ هﺪﺷ ﺎﻫ
ي زا ﺮﺘﻤﮐ 600
ﻣ راﺰﻫ ﯿﻠ ﯽ ﻟ ﺮﺑ مﺮﮔ ﯿ
ﺮﺘ ترﺪﻗ ﺪﻗﺎﻓ
ژﺮﭙﺳآﺪﺿ ﯾ ﺳﻮﻠ ﯽ دﻮﺑ .
ﻪﻠﺠﻣ مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﯾ ناﺮ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ
ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ
1 - ﻪﻣﺪﻘﻣ
ﺘﺧرد زا رﺪﻨﮐ ﻎﻤﺻ ﯽ
ﻤﻠﻋ مﺎﻧ ﺎﺑ ﯽ
Boswellia serrata ﻪﺑ
ﺖﺳد ﻣ ﯽ آﯾ ﻔﻠﺘﺨﻣ ﻖﻃﺎﻨﻣ رد ﻪﮐ ﺪ ﯽ
ﺮﻓآ قﺮﺷ زا ﯾ
ﻣروﺎﺧ ،ﺎﻘ ﯿ و ﻪﻧﺎ
د زا و هﺪﺷ ﺖﺸﮐ ﺪﻨﻫ ﯾ
ﻧﺎﻣرد فاﺪﻫا ﺎﺑ زﺎﺑﺮ ﯽ
ا رد ﯾ درﻮﻣ ﻖﻃﺎﻨﻣ ﻦ
ﺖﺳا هدﻮﺑ هدﺎﻔﺘﺳا .
ا ﺐﻃ رد ﯾ ﻧاﺮ ﯽ ﺪﻨﻫ و ي ﺪﻗ ﯾﻤ ﯽ ﺻﺎﺧ زا ﯿ ﺖ
ﺑﺎﻬﺘﻟاﺪﺿ ﯽ ﺴﺑ رﺪﻨﮐ هرﺎﺼﻋ و ﻎﻤﺻ ﯿ
رد و ﺖﺳا هﺪﺷ هدﺎﻔﺘﺳا رﺎ
ﺪﻨﭼ ﯾ ﻧﺎﻣرد صاﻮﺧ ﻪﺑ ﺶﻫوﮋﭘ ﻦ ﯽ
اﺮﺑ نآ ي ﻣرد ﺮﺗرآ ،ﻢﺳآ نﺎ ﯾ
ﺖ
ﺋﻮﺗﺎﻣور ﯿ ﺮﺗرآﻮﺌﺘﺳا و ﺪ ﯾ
ﺖﺳا هﺪﺷ هرﺎﺷا ﺖ 1]
- 3 .[ نآ ﺮﺑ هوﻼﻋ
ﺧﺮﺑ ﺮﺑاﺮﺑ رد رﺪﻨﮐ ﻎﻤﺻ ﯽ
ﺰﻧآ ﯾ ﻢ ﻟ ﺪﻨﻧﺎﻣ ﺎﻫ ﯿ
ﺴﮐﻮﭙ ﯽ اراد زﺎﻧژ ي
ﺻﺎﺧ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖ ﯽ
ﻧ ﯿ ﻣ ﺰ ﯽ ﺪﺷﺎﺑ 4] - 6 .[ ﺑﺎﻬﺘﻟاﺪﺿ صاﻮﺧ ﯽ
ﻎﻤﺻ
ﻪﺑ رﺪﻨﮐ ﻟﻮﺳﻮﺑ ﯿ ﺳا ﮏ ﯿ
1ﺪ ﻣ ﻪﮐ ﺖﺳا طﻮﺑﺮﻣ ﯽ
ﺎﺑﯾ هﺪﺷ جاﺮﺨﺘﺳا ﺖﺴ
ﺎﺗ ﺸﺨﺑﺮﺛا ﯽ ﺑ نآ ﯿ دﻮﺷ ﺮﺘﺸ 7]
.[ ﺎﺳ نآ ﺮﺑ هوﻼﻋ ﯾ
ﮐﺮﺗ ﺮ ﯿ ﺪﻨﻧﺎﻣ تﺎﺒ
ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻧ ﯿ اراد ﺰ ي ﺴﮐاﺪﺿ صاﻮﺧ ﯿ
ﻧاﺪ ﯽ ﻣﺪﺿ و ﯿ ﺑوﺮﮑ ﯽ
ﻣ ﯽ ﺪﻨﺷﺎﺑ 8] .[
ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ شور ﻪﺑ ﺎﻫ ي ﻔﻠﺘﺨﻣ ﯽ ﮔ ﻊﺑﺎﻨﻣ زا ﯿ
ﻫﺎ ﯽ جاﺮﺨﺘﺳا
ﻣ ﯽ اﺮﺑ و ﺪﻧﻮﺷ ي
ا ﯾ شور زا ﻻﻮﻤﻌﻣ رﺎﮐ ﻦ ﺎﻫ
ي ﺘﻨﺳ ﯽ هدﺎﻔﺘﺳا
ﻣ ﯽ ﺖﺳا هﺪﺷ .
لﺎﺳ رد ﺎﻣا ﺎﻫ
ي ﺧا ﯿ اﺮﮔ ﺮ ﯾ زا هدﺎﻔﺘﺳا ﻪﺑ ﺶ
شور ﺎﻫ ي ﺖﺳود ﺤﻣ راد ﯿ ز ﻂ ﯾ ا و ﺖﺴ ﯾ اﺰﻓا ﻦﻤ ﯾ ﺶ ﯾ ﺖﺳا ﻪﺘﻓﺎ . زا
ا ﻪﻠﻤﺟ ﯾ شور ﻦ ﻣ ﺎﻫ ﯽ ﻧاﺮﺤﺑ نودﺎﻣ جاﺮﺨﺘﺳا ﻪﺒﻧاﻮﺗ ﯽ
شور ،بآ
ﺎﻣ ﯾ ووﺮﮑ ﯾ ﻣ ،تﻮﺻاﺮﻓ ،ﻮ ﯿ
ناﺪ ﺎﻫ ي ﺮﺘﮑﻟا ﯾ ﮑ ﯽ ﺴﻟﺎﭘ ﯽ نودﺎﻣ بآ ،
ﻧاﺮﺤﺑ ﯽ و ...
دﻮﻤﻧ هرﺎﺷا 9]
و 10 .[
ﻧاﺮﺤﺑ نودﺎﻣ بآ جاﺮﺨﺘﺳا شور
2ﯽ ) (SCW ﯾ جاﺮﺨﺘﺳا شور ﮏ
ﭘ ،ﺰﺒﺳ ﯿ دﺎﺼﺘﻗا ﻪﻓﺮﺻ ﺎﺑ و ﻪﺘﻓﺮﺸ ي
) techno-economically (
ﻣ ﯽ ﻠﺑﺎﻗ ﻪﮐ ﺪﺷﺎﺑ ﯿ
ﺎﺟ ﺖ ﯾ ﺰﮕ ﯾﻨ ﯽ شور ﺎﺑ ﺎﻫ ي جاﺮﺨﺘﺳا لواﺪﺘﻣ
ﻣ اراد ار لﻼﺣ ﻂﺳﻮﺗ ﯽ
ﺪﺷﺎﺑ . ارد ﯾ ﻪﺑ بآ زا شور ﻦ لﻼﺣ ناﻮﻨﻋ
ﻣ هدﺎﻔﺘﺳا ﯽ دﻮﺷ . ﻧاﺮﺤﺑ نودﺎﻣ بآ ﻂﺳﻮﺗ جاﺮﺨﺘﺳا شور رد ﯽ
، ا ز
ﻪﺑ بآ ﻣ هدﺎﻔﺘﺳا جاﺮﺨﺘﺳا لﻼﺣ ناﻮﻨﻋ ﯽ
دﻮﺷ . بآ ﯾ رد لﻼﺣ ﮏ
ﻏ ،نازرا ،سﺮﺘﺳد ﯿ
ﺪﺠﺗ ،لﺎﻌﺘﺷا ﻞﺑﺎﻗ ﺮ ﯾ
ﺬﭘ ﺪ ﯾ ﻏ ،ﺮ ﯿ ﻤﺳﺮ ﯽ ﺮﺑ و هدﻮﺑ
ﺤﻣ ﯿ ز ﻂ ﯾ ﻣ ﻮﺳ تاﺮﺛا ﺪﻗﺎﻓ ﺖﺴ ﯽ
ﺪﺷﺎﺑ . د زا ﯾ اﺰﻣ ﺮﮕ ﯾﺎ ي بآ شور
ﻧاﺮﺤﺑ نودﺎﻣ ﯽ
ﺎﻘﻣ رد ﯾ شور ﺎﺑ ﻪﺴ ﺎﻫ
ي ﺘﻨﺳ ﯽ ،جاﺮﺨﺘﺳا مﻮﺳﺮﻣ و
ﻣ ﯽ هﺎﺗﻮﮐ نﺎﻣز ﻪﺑ ناﻮﺗ ﺳا ﺮﺗ
نآ ﺮﺗﻻﺎﺑ جاﺮﺨﺘﺳا نﺎﻣﺪﻧار و جاﺮﺨﺘ
دﻮﻤﻧ هرﺎﺷا 11]
- 14 .[
1boswellic acid 2Subcritical Water
ﻧاﺮﺤﺑ نودﺎﻣ بآ ﯽ
ﺳ ، ﯿﻟﺎ ﯽ د ﺖﺑﺎﺛ ﻪﮐ ﺖﺳا ي
ﺮﺘﮑﻟا ﯾ رﻮﻃ ﻪﺑ نآ ﮏ
ﮕﻤﺸﭼ ﯿﺮ ي ﻣ ﺶﻫﺎﮐ ﯽ ﯾ ﺪﺑﺎ . د ﺖﺑﺎﺛ ي ﺮﺘﮑﻟا ﯾ ﮕﺘﺴﺑاو بآ ﮏ ﯽ
ﻻﺎﺑ ﯾ ﯽ ﺒﻄﻗ ﻪﺑ ﯿ ﺎﻣد و ﺖ ي ز ﯾ ﻧاﺮﺤﺑ ﺮ ﯽ ) under elevated (
اﺮﻓ ﯾ ﺪﻨ
دراد SCW . د ﺖﺑﺎﺛ ﺶﻫﺎﮐ ي
ﺮﺘﮑﻟا ﯾ صاﻮﺧ ،بآ ﮏ ﻟﻼﺤﻧا
ﯽ نآ
اﺰﻓا ار ﯾ ﻣ ﺶ ﯽ ﺪﻫد . ا زا ﯾ ﮐﺮﺗ وﺮﻨ ﯿ ﻏ تﺎﺒ ﯿ ﺒﻄﻗﺮ ﯽ نودﺎﻣ بآ رد
ﻧاﺮﺤﺑ ﯽ ﻠﺑﺎﻗ ، ﯿ ﺑ لﻼﺤﻧا ﺖ ﯿ
ﺮﺘﺸ ي ﭘ ﯿ ﻣ اﺪ ﯽ ﺪﻨﻨﮐ . خﺮﻧ نآ ﺮﺑ هوﻼﻋ
و ﺶﻫﺎﮐ ﺮﺛا رد ،مﺮﺟ لﺎﻘﺘﻧا ﯾ
زﻮﮑﺴ ﯾ ﺤﻄﺳ ﺶﺸﮐ و ﻪﺘ ﯽ
،بآ
اﺰﻓا ﯾ ﺶ ﯾ اﺰﻓا ﻪﺑ ﺮﺠﻨﻣ و ﻪﺘﻓﺎ ﯾ
ﺮﺿ ﺶ ﯾ ﻣ لﻼﺣ ذﻮﻔﻧ ﺐ ﯽ
ددﺮﮔ
15] - 18 .[
ﺳﻻﻮﺴﭙﮑﻧا ﯿ نﻮ ﻨﮑﺗ ﯿ ﮑ ﯽ ﺎﻨﺻ رد ﻪﮐ ﺖﺳا ﯾ
ﻔﻠﺘﺨﻣ ﻊ ﯽ و دراد دﺮﺑرﺎﮐ
ﺶﺨﺑ رد ﺎﻫ ي ﺰﻧآ ﯾ زﺎﺳ ﻢ ي ﻟﻮﺗ ، ﯿ لﻮﻠﺳ ﺪ و ﺎﻫ ﯾ ﻪﺘﺧﺎ ﻘﻣ رد ﺎﻫ ﯿ سﺎ
ﺴﺑ ﯿ ر رﺎ ﯾ دراد دﺮﺑرﺎﮐ ﺰ .
ﻟد ﻪﺑ ﯿ ا ﻞ ﯾ ﮐﺮﺗ ﻪﮑﻨ ﯿ ار هﺪﺷ رﻮﺼﺤﻣ تﺎﺒ
ﻣ ﯽ ﺴﮐا ،ﺖﺑﻮﻃر ،ﺎﻣﺮﮔ ﺮﺑاﺮﺑ رد ناﻮﺗ ﯿ
ﺎﺳ و نﮋ ﯾ اﺮﺷ ﺮ ﯾ ﺖﻈﻓﺎﺤﻣ ﻂ
ﺳﻻﻮﺴﭙﮑﻧا دﺮﺑرﺎﮐ ،دﻮﻤﻧ ﯿ
اﺰﻓا نﻮ ﯾ ﺶ ﯾ ﻪﺘﻓﺎ ﺖﺳا . ا زا هدﺎﻔﺘﺳا ﯾ
ﻦ
ژﻮﻟﻮﻨﮑﺗ ي اﺰﻓا ﺚﻋﺎﺑ ﯾ
رﺎﮔﺪﻧﺎﻣ ﺶ ي
ﺻﻮﺼﺧ ﻆﻔﺣ و لﻮﺼﺤﻣ ﯿ
تﺎ
ﻓﯿ ﺰﯾ ﺷﻮﮑ ﯿﻤ ﯿﺎﯾ ﯽ ﺴﺣ صاﻮﺧ ﺶﻫﺎﮐ زا و هﺪﺷ نآ ﯽ
ﺘﭙﻟﻮﻧﺎﮔرا و ﯿ
ﮏ
ﮔﻮﻠﺟ نآ ﯿﺮ ي ﻣ ﯽ ﺎﻤﻧ ﯾ ﺪ 19] .[
ﻪﻟﻮﺴﭙﮐ زﺎﺳ ي و ﻣ ﯿ ﺳوﺮﮑ ﯿ لﺎ زﺎﺳ ي ﺮﻫ ود ﻞﻣﺎﺷ ﮐﺮﺗ ﯿ ﺐ داﻮﻣ
اﺬﻏ ﯾ ﯽ
، ﺰﻧآ ﯾ
،ﺎﻬﻤ ﺎﻬﻟﻮﻠﺳ ﯾ ﺎ ﺎﺳ ﯾﺮ داﻮﻣ رد ﺎﻬﻟﻮﺴﭙﮐ ي ﮏﭼﻮﮐ ﺳا ﺖ .
ﺎﺑ اﯾ
،لﺎﺤﻨ ﺎﻫدﺮﺑرﺎﮐ ي ﻪﻟﻮﺴﭙﮐ زﺎﺳ ي رد ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯾ ﯽ اﺰﻓا ﯾ ﺶ ﯾ ﻪﺘﻓﺎ
ﺖﺳا ز ﯾ اﺮ داﻮﻣ رﻮﺼﺤﻣ هﺪﺷ ار ﻣ ﯽ ناﻮﺗ رد ﺮﺑاﺮﺑ
،ﺖﺑﻮﻃر ﺎﻣﺮﮔ ﯾ ﺎ
ﺎﺳ ﯾﺮ اﺮﺷ ﯾ ﻂ ﺪﺷ ﯾ ﺪ ﺖﻈﻓﺎﺤﻣ و دﺮﮐ ﺎﭘ ﯾ راﺪ ي و رﺎﮔﺪﻧﺎﻣ ي ار ﻆﻔﺣ
دﻮﻤﻧ . ﻪﻟﻮﺴﭙﮐ ندﺮﮐ ﺎﻬﻨﻏور ي ﮐارﻮﺧ ﯽ ﻂﺳﻮﺗ تارذ
،ردﻮﭘ وﺎﺣ ي
ﻫﻮﺑﺮﮐ ﯿ تارﺪ ﯾ ﺎ ﺌﺗوﺮﭘ ﯿ
،ﻦ ﯾ ﮏ آﺮﻓ ﯾ ﺪﻨ ﻤﻠﻋ ﯽ ﺖﺳا ﻪﮐ اﺮﺑ ي
ﺖﻈﻓﺎﺤﻣ زا ﺎﻬﻨﻏور ي ﻏﺪﻨﭼ ﯿ عﺎﺒﺷاﺮ زا ﺴﮐا ﯿ ﺳاﺪ ﯿ نﻮ و رد ﺘﻧ ﯿ ﻪﺠ
ﻆﻔﺣ ﺎﻬﻤﻌﻃ لﺎﺒﻧد ﻣ ﯿ دﻮﺸ .
زا اﯾ ﻦ ور ا زا فﺪﻫ ﯾ ﮐﺮﺗ جاﺮﺨﺘﺳا ﻪﻌﻟﺎﻄﻣ ﻦ ﯿ
ﻪﺑ رﺪﻨﮐ زا هﺮﺛﻮﻣ تﺎﺒ
شور ﺳرﺮﺑ و SCW
ﯽ ﺻﺎﺧ ﯿ ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣﺪﺿ ، ﯿ ﺑوﺮﮑ ﯽ و
ژﺮﭙﺳآﺪﺿ ﯾ ﺳﻮﻠ ﯽ هرﺎﺼﻋ ﺎﻫ ي ﻨﭽﻤﻫ و نآ ﯿ
ﺎﻘﻣ ﻦ ﯾ ﻪﺴ ﺎﺘﻧ ﯾ شور ﺞ
جاﺮﺨﺘﺳا ﺘﻨﺳ شور ﺎﺑ SCW
ﯽ ﺧ ﯿ دﻮﺑ نﺪﻧﺎﺴ .
نآ ﺮﺑ هوﻼﻋ
ﮐﺮﺗ توﺎﻔﺗ ﯿ
ا رد هﺪﺷ جاﺮﺨﺘﺳا تﺎﺒ ﯾ
ﻧ شور ود ﻦ ﯿ
ﺳرﺮﺑ درﻮﻣ ﺰ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
2 - شور و داﻮﻣ ﺎﻫ
2 - 1 - ﻬﺗ ﯿ ﻟوا داﻮﻣ ﻪ ﯿﻪ
ﻣﺎﻤﺗ ﯽ لﻮﻠﺤﻣ ﺎﻫ ي ﺷ ﯿﻤ ﯿﺎﯾ ﯽ ﺤﻣ و ﯿ ﻂ ﺎﻫ ي كﺮﻣ ﺖﮐﺮﺷ زا ﺖﺸﮐ
شﻮﺳ ،نﺎﻤﻟآ ﺎﻫ
ي ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﻣ ﮏﻧﺎﺑ زا ﯿ
ﺑوﺮﮑ ﯿﻮ ژﻮﻟ ي نﺎﻣزﺎﺳ
ﺶﻫوﮋﭘ ﺎﻫ ي ﻤﻠﻋ ﯽ ﺘﻌﻨﺻ و ﯽ ا ﯾ د و ناﺮ ﯾ ﮏﺴ ﺎﻫ ي ﺎﻣﺎﺘﻨﺟ ﯾ ﺴ ﯿ زا ﻦ
ﻬﺗ ﺐﻃ ﻦﺗدﺎﭘ ﺖﮐﺮﺷ ﯿ
دﺮﮔ ﻪ ﯾ ﺪ.
2 - 2 - هرﺎﺼﻋ ﮔﯿ ﺮ ي ﻪﺑ شور ﺧ ﯿ نﺪﻧﺎﺴ
ﺖﺒﺴﻧ 1:30 زا بآ ﺮﻄﻘﻣ و رﺪﻨﮐ ﻬﺗ ﯿ ﺪﺷ ﻪ و ﻪﺑ تﺪﻣ 18 ﺖﻋﺎﺳ
ور ي نﺰﻤﻫ ﻃﺎﻨﻐﻣ ﯿ ﺴ ﯽ راﺮﻗ ﺖﻓﺮﮔ . ﺲﭘ زا نآ هرﺎﺼﻋ
،ﻞﺻﺎﺣ
فﺎﺻ و نورد فوﺮﻇ موﺎﻘﻣ رد ﺮﺑاﺮﺑ ذﻮﻔﻧ رﻮﻧ راﺪﻬﮕﻧ ي ﺪﺷ 20] .[
2 - 3 - هرﺎﺼﻋ ﮔﯿ ﺮ ي ﺎﺑ هدﺎﻔﺘﺳا زا
بآ نودﺎﻣ
ﻧاﺮﺤﺑ ﯽ
ارد ﯾ ﻦ شور ﺖﺒﺴﻧ 1:30 زا بآ ﺮﻄﻘﻣ و رﺪﻨﮐ ﻬﺗ ﯿﻪ و ﺎﺑ هدﺎﻔﺘﺳا زا
هﺎﮕﺘﺳد جاﺮﺨﺘﺳا هﺪﻨﻨﮐ نودﺎﻣ ﻧاﺮﺤﺑ ﯽ بآ ) ﺣاﺮﻃ ﯽ هﺪﺷ ﻪﺘﺧﺎﺳ و
ﺎﻣزآ رد ﯾ روﺎﻨﻓ هﺎﮕﺸ ي
ﺎﻫ ي ﻮﻧ ﯾ ﺸﻫوﮋﭘ ﻪﺴﺳﻮﻣ ﻦ ﯽ
ﺎﻨﺻ و مﻮﻠﻋ ﯾ
ﻊ
اﺬﻏ ﯾ ﯽ ا ،ﺪﻬﺸﻣ ، ﯾﺮ نا (،
) رد ﺎﻫﺎﻣد ي 120 و 160 ﻪﺟرد ﺘﻧﺎﺳ ﯽ داﺮﮔ ﻪﺑ
تﺪﻣ 5 و 15 ﻗد ﯿ ﻪﻘ ( مﺎﺠﻧا و هرﺎﺼﻋ ﻞﺻﺎﺣ فﺎﺻ و نورد فوﺮﻇ
موﺎﻘﻣ رد ﺮﺑاﺮﺑ ذﻮﻔﻧ رﻮﻧ راﺪﻬﮕﻧ ي دﺮﮔ ﯾ ﺪ 15] .[
2 - 4 - ﻟﻮﺗ ﯿ ر ردﻮﭘ ﺪ ﯾ
ﻧﺎﺷﻮﭘﺰ ﯽ ﻎﻤﺻ هرﺎﺼﻋ هﺪﺷ
رﺪﻨﮐ
اﺮﺑ ي اﯾ ﻦ رﻮﻈﻨﻣ زا ﺮﺘﺴﮐدﻮﺘﻟﺎﻣ ﯾ
ﻦ ﻪﺑ هدﺎﻣ ناﻮﻨﻋ د
ﯾ هراﻮ و ﺖﻬﺟ
اﯾ دﺎﺠ ﯾ ﮏ ﺶﺷﻮﭘ د ر فاﺮﻃا ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ ي جاﺮﺨﺘﺳا هﺪﺷ زا
رﺪﻨﮐ هدﺎﻔﺘﺳا ﺪﺷ . ﺖﻈﻠﻏ 20 ﺪﺻرد زا هرﺎﺼﻋ رﺪﻨﮐ وﺎﺣ ي
ﺮﺘﺴﮐدﻮﺘﻟﺎﻣ ﯾ
ﻦ ﻬﺗ ﯿﻪ و ﺎﺑ هدﺎﻔﺘﺳا زا نﺰﻤﻫ ﻃﺎﻨﻐﻣ ﯿ ﺴ ﯽ ﻪﺑ ﺑﻮﺧ ﯽ
ﻦﮕﻤﻫ دﺮﮔ ﯾ ﺪ . لﻮﻠﺤﻣ ﻞﺻﺎﺣ ﻂﺳﻮﺗ ﮏﺸﺧ ﻦﮐ ﺸﺷﺎﭘ ﯽ
) Buchi, B-191, Switzerland (
رد ﺎﻣد ي 160 - 180 ﻪﺟرد
ﺘﻧﺎﺳ ﯿ داﺮﮕ ﮏﺸﺧ و ردﻮﭘ ﻞﺻﺎﺣ نورد فوﺮﻇ موﺎﻘﻣ رد ﺮﺑاﺮﺑ ذﻮﻔﻧ
ﺖﺑﻮﻃر و رﻮﻧ ﺎﺗ نﺎﻣز فﺮﺼﻣ راﺪﻬﮕﻧ ي ﺪﺷ 19] .[
2 - 5 - هزاﺪﻧا ﮔﯿ ﺮ ي جاﺮﺨﺘﺳا نﺎﻣﺪﻧار
هرﺎﺼﻋ نﺎﻣﺪﻧار ﮔﯿ
ﺮ ي ﻪﻄﺑار زا هدﺎﻔﺘﺳا ﺎﺑ 1
ﺪﺷ ﻪﺒﺳﺎﺤﻣ :
ﻪﻄﺑار ) 1 ( Extraction Efficiency% = (m1-m0)/m2×100
نآ رد ﻪﮐ m0
ﻠﭘ نزو ﯿ ﻟﺎﺧ ﺖ ﯽ ، m1
ﻠﭘ نزو ﯿ ﻧﻮﻤﻧ و ﺖ ﮏﺸﺧ ﻪ
و هﺪﺷ m2
ﻠﭘ نزو ﯿ ﻣ ﻪﻧﻮﻤﻧ و ﺖ ﯽ
ﺪﺷﺎﺑ 20] .[
2 - 6 - ﻣ ﺶﺠﻨﺳ ﯿ
ﮐﺮﺗ ناﺰ ﯿ ﻨﻓ تﺎﺒ ﻮﻟ ﯽ
3
ﻞﮐ ) ( TPC
اﺮﺑ ي ﻌﺗ ﯿﯿ ﻦ لﻮﻨﻓ ،ﻞﮐ 5/
0 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ زا هرﺎﺼﻋ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺎﺑ
5/
2 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ فﺮﻌﻣ ﻟﻮﻓ ﯿ ﻦ 10 % و 2 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ لﻮﻠﺤﻣ تﺎﻨﺑﺮﮐ
ﺪﺳ ﯾ ﻢ 5/
7 % طﻮﻠﺨﻣ هﺪﺷ و ﺲﭙﺳ بﺬﺟ هﺎﮕﺘﺳد ﻂﺳﻮﺗ لﻮﻠﺤﻣ
ﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا )
Shmadzu, UV-160A, Japan (
رد لﻮﻃ
جﻮﻣ 760 ﺮﺘﻣﻮﻧﺎﻧ ﺖﺋاﺮﻗ دﺮﮔ ﯾ ﺪ . اﺮﺑ ي ﻪﺒﺳﺎﺤﻣ راﺪﻘﻣ ﮐﺮﺗ ﯿ تﺎﺒ
ﻟﻮﻨﻓ ﯽ ﺐﺴﺣﺮﺑ ﻣ ﯿﻠ ﯽ مﺮﮔ ﻟﺎﮔ ﯿ ﮏ ﺳا ﯿ ﺪ رد 100 مﺮﮔ هدﺎﻣ ﮔ ﯿ ﻫﺎ ﯽ
، زا
ﻨﺤﻨﻣ ﯽ دراﺪﻧﺎﺘﺳا ﺳﺮﺗ
ﯿ ﻢ هﺪﺷ رد ﺖﻈﻠﻏ ﺎﻫ ي 0 ﺎﺗ 200ppm
هدﺎﻔﺘﺳا ﺪﺷ 21] .[
2 - 7 - ﺳرﺮﺑ ﯽ رﺪﻗ دار ت ﯾ لﺎﮑ ﮔ ﯿ ﮔﺪﻧﺮ ﯽ
اﺮﺑ ي اﯾ ﻦ رﻮﻈﻨﻣ ﺖﻈﻠﻏ ﺘﺑﺎﺛ ﯽ زا هرﺎﺼﻋ ﺎﻫ ي رﺪﻨﮐ ﻬﺗ ﯿﻪ و 3
ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ زا نآ ﺎﺑ 1 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ زا لﻮﻠﺤﻣ DPPH
) 500 ﻣ ﯿ رﻻﻮﻣوﺮﮑ ( طﻮﻠﺨﻣ و ﺲﭘ زا 30 ﻗد ﯿ ﻪﻘ راﺮﻗ ﻦﺘﻓﺮﮔ رد
نﺎﮑﻣ رﺎﺗ ﯾ
،ﮏ بﺬﺟ لﻮﻠﺤﻣ رد لﻮﻃ جﻮﻣ 517 ﺮﺘﻣﻮﻧﺎﻧ ﺖﺋاﺮﻗ
ﺪﺷ 15] .[
ر ﻪﻄﺑا ) 2 ( RSA% = (Ablank – Asample / Ablank) × 100
رد اﯾ ﻦ لﻮﻣﺮﻓ Ablank بﺬﺟ
ﻪﻧﻮﻤﻧ
،ﺪﻫﺎﺷ Asample
بﺬﺟ هرﺎﺼﻋ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ .
2 - 8 - ﺳرﺮﺑ ﯽ ﻣ ﺪﺿ ترﺪﻗ ﯿ
ﺑوﺮﮑ ﯽ
2 - 8 - 1 - ﻌﺗ ﯿﯿ ﻦ ﻞﻗاﺪﺣ ﺖﻈﻠﻏ ﺖﻌﻧﺎﻤﻣ هﺪﻨﻨﮐ
زا ﺪﺷر
ﺮﺘﮐﺎﺑ ي (MIC)
ﺖﻬﺟ زرا ﯾﺑﺎ ﯽ ﺺﺧﺎﺷ )
(MIC اﺪﺘﺑا ﺖﺸﮐ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ زا
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﺷﺮﺷا ﯿ ﻠﮐﺎ ﯽ 46389 ATCC و
ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ
سﻮﺋروا 9538 رد ATCC ﺤﻣ ﯿ ﻂ ﺖﺸﮐ ﻫﺮﻟﻮﻣ ﯿ نﻮﺘﻨ ﻪﺑ تﺪﻣ 24
ﺖﻋﺎﺳ اﺮﺑ ي ﻣﺎﻤﺗ ﯽ ﺮﺘﮐﺎﺑ ي ﺎﻫ ي رﻮﮐﺬﻣ مﺎﺠﻧا ﺪﺷ . ﺲﭙﺳ اﺮﺑ ي ﻬﺗ ﯿﻪ
ﺴﻧﺎﭙﺳﻮﺳ ﯿ نﻮ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﺎﺑ دراﺪﻧﺎﺘﺳا 5/
0 ﮏﻣ
،ﺪﻨﻟرﺎﻓ اﺪﺘﺑا
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻟﯿ هﺰﯿﻠﻓﻮ ﻪﺑ رﻮﻃ ﻪﻧﺎﮔاﺪﺟ ور
ي ﺤﻣ ﯿ ﻂ ﺖﺸﮐ
ﻫﺮﻟﻮﻣ ﯿ نﻮﺘﻨ ثاﺮﺑ ) كﺮﻣ نﺎﻤﻟآ ( ﻘﻠﺗ ﯿ ﺢ و ﺖﺸﮐ ﻣ ﯿ ﺑوﺮﮑ ﯽ ﻪﺑ رﻮﻈﻨﻣ
ﺳر ﯿ نﺪ ﻪﺑ زﺎﻓ رﺎﮕﻟ ﯾ ﻤﺘ ﯽ ﻪﺑ تﺪﻣ 24 ﺖﻋﺎﺳ رد رﻮﺗﺎﺑﻮﮑﻧا 37
3 Total Phenolic Compounds (TPC)
ﻪﺟرد ﺘﻧﺎﺳ ﯽ داﺮﮔ راﺮﻗ هداد ﺪﺷ 21] .[ رد ﻪﻧﻮﻤﻧ بﺬﺟ نآ زا ﺲﭘ
جﻮﻣ لﻮﻃ 620
ﻻا هﺎﮕﺘﺳد رد ﺮﺘﻣﻮﻧﺎﻧ ﯾ
ر اﺰ ﯾ رﺪ ) Cormate,
State Fax 4300, USA (
دﺮﮔ ﺖﺋاﺮﻗ ﯾ
ﺪ.
2 - 8 - 2 - ﻌﺗ ﯿﯿ ﻦ ﻞﻗاﺪﺣ ﺖﻈﻠﻏ ﮔﺪﻨﺸﮐ ﯽ (MBC)
ﻪﺑ ﺳرﺮﺑ رﻮﻈﻨﻣ ﯽ
ﻞﻗاﺪﺣ ﺖﻈﻠﻏ ﮔﺪﻨﺸﮐ ﯽ
، ﻪﺑ ﻣ ﯿ ناﺰ 100 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺮﺘ
زا ﺖﻈﻠﻏ ﺎﻫ ي ﻌﺗ ﯿﯿ ﻦ هﺪﺷ رد شور
، MIC ﻪﺑ رﻮﻃ ﻪﻧﺎﮔاﺪﺟ ور ي
ﺤﻣ ﯿ ﻂ ﺖﺸﮐ ﻫﺮﻟﻮﻣ ﯿ نﻮﺘﻨ رﺎﮔآ ﻞﻘﺘﻨﻣ دﺮﮔ ﯾ ﺪ و ﻪﺑ تﺪﻣ 24 ﺖﻋﺎﺳ
رد رﻮﺗﺎﺑﻮﮑﻧا 37 ﻪﺟرد ﺘﻧﺎﺳ ﯽ داﺮﮔ راﺮﻗ هداد ﺪﺷ 21] .[
2 - 8 - 3 - ﻌﺗ ﯿﯿ ژﺮﭙﺳآ ﺪﺿ ترﺪﻗ ﻦ ﯾ
ﺳﻮﻠ ﯽ ر ردﻮﭘ ﯾ ﻧﺎﺷﻮﭘﺰ ﯽ
هﺪﺷ
اﺮﺑ ي ﻌﺗ ﯿﯿ ﭼرﺎﻗ ﺪﺿ ترﺪﻗ ﻦ ﯽ
) (MFC ﻪﺑ هرﺎﺼﻋ زا هﺪﻣآ ﺖﺳد
ﻬﺑ ﻪﻄﻘﻧ ﯿ ،ﻪﻨ 10 ﻣ ﯿ ﻟوﺮﮑ ﯿ ﺴﻧﺎﭙﺳﻮﺳ زا ﺮﺘ ﯿ
ﭼرﺎﻗ نﻮ ﯽ 5 /0 ﺪﻨﻟرﺎﻓ ﮏﻣ
ﺤﻣ ﻪﺑ ﯿ ﺖﺸﮐ ﻂ ) PDA
Potato Dextrose Agar (
وﺎﺣ ي
ﺖﻈﻠﻏ ﺎﻫ ي 300 و 600 ﻣ راﺰﻫ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟ ﯿ ﻋ زا ﺮﺘ هرﺎﺼ
ﻪﻓﺎﺿا ،رﺪﻨﮐ ﻘﻠﺗ زا ﺲﭘ و
ﯿ تﺪﻣ ﻪﺑ ﺎﺑ ﺢ 72
ﺎﻣد رد ﺖﻋﺎﺳ ي
25
ﺘﻧﺎﺳ ﻪﺟرد ﯽ راﺪﻬﮕﻧ داﺮﮔ ي
ﺪﺷ 21] [
2 - 9 - ﺳرﺮﺑ ﯽ ﺻﻮﺼﺧ ﯿ
ر ردﻮﭘ تﺎ ﯾ
ﻧﺎﺷﻮﭘﺰ ﯽ هﺪﺷ
2 - 9 - 1 - زاﺪﻧا يﺮﯿﮔ هزاﺪﻧا ه
ﺺﺧﺎﺷ و نﻮﯿﺴﻟﻮﻣا تارذ
ﯽﮔﺪﯿﺷﺎﭘ ﺲﺑ ﻂﺳﻮﺘﻣ
،ﺮﻄﻗ زﻮﺗ ﯾ ﻊ هزاﺪﻧا و ﺢﻄﺳ صﻮﺼﺨﻣ تارذ
ﺎﺑ ﮏﻤﮐ
هﺎﮕﺘﺳد ﺶﺠﻨﺳ هزاﺪﻧا ارذ ت ) Fritsch Analysette,
Germany (
ﺻﻮﺗ شور ﺎﺑ و ﯿ
رﺎﮑﮔورﻮﻣ ﻂﺳﻮﺗ هﺪﺷ ﻒ و 4
نارﺎﮑﻤﻫ ) 2019 ( مﺎﺠﻧا ﺪﺷ 19] . [ اﯾ ﻦ هﺎﮕﺘﺳد ﺮﻄﻗ تارذ ار ﺮﺑ
سﺎﺳا يرﻮﺌﺗ وMie زا يور ﺶﻨﮐاﺮﭘ ﻪﻌﺷا ﻟﯿ رﺰ ﻪﺒﺳﺎﺤﻣ هدﻮﻤﻧ و
ﻣﯿ ﮕﻧﺎ ﯿ ﻦ ﺮﻄﻗ ار ﺖﺤﺗ ناﻮﻨﻋ دﺪﻋ
5ﺮﺗﺎﺳ ﯾ ﺎ ﻣﯿ ﮕﻧﺎ ﯿ ﻦ ﺮﻄﻗ ﺢﻄﺳ نزو-
) D3,2
( ﻪﺑ ﺖﺳد ﻣ ﯽ ﺪﻫد . ﺢﻄﺳ صﻮﺼﺨﻣ تارذ
) (SSA ﻧﯿ ﺰ
ﺐﺴﺣﺮﺑ m2/ml زا يور ﺮﻄﻗ ﻂﺳﻮﺘﻣ ﻪﺑ ترﻮﺻ ذ ﯾ ﻞ ﻪﺒﺳﺎﺤﻣ
ﺪﺷ : ﻪﻄﺑار 3 D3,2= ∑zidi
3/∑zidi
2
ﻪﻄﺑار 4 SSA=6/D3,2
ﺮﻄﻗ ﻂﺳﻮﺘﻣ تارذ ﻪﮐ دﺎﻤﻧ ﺎﺑ D4,3
) لﻮﻃ ﻪﺑ ﻢﺠﺣ ﺮﻄﻗ (
ﺎﻤﻧ ﯾ ﺶ
ﻣ هداد ﯽ ز ﻪﻟدﺎﻌﻣ زا هدﺎﻔﺘﺳا ﺎﺑ ،دﻮﺷ ﯾ
دﺮﮔ ﻪﺒﺳﺎﺤﻣ ﺮ ﯾ
ﺪ:
4Murugkar 5Sauter
ر ﻪﻄﺑا 5 D4,3= ∑zidi4
/∑zidi3
رﻮﮐﺬﻣ ﻪﻟدﺎﻌﻣ رد Zi
ﺮﻄﻗ ﺎﺑ تارذ داﺪﻌﺗ di
ﻣ ﯽ ﺪﺷﺎﺑ .
اﺮﺑ ي ﺎﻘﻣ ﯾ ﻪﺟرد ﻪﺴ ﯾ
ﺘﺧاﻮﻨﮑ ﯽ ﻪﻧﻮﻤﻧ تارذ هزاﺪﻧا ﺼﺧﺎﺷ زا ﺎﻫ
ﯽ ﻪﺑ
مﺎﻧ ز ﻪﻟدﺎﻌﻣ زا ﻪﮐ Span ﯾ
ﻣ ﻪﺒﺳﺎﺤﻣ ﺮ ﯽ
ﺪﺷ هدﺎﻔﺘﺳا ،دﻮﺷ :
ﻪﻄﺑار 6 Span= [d(v,90)-d(v,10)]/d(v,50)
ا رد ﯾ ﻪﻟدﺎﻌﻣ ﻦ d(v,90)
، d(v,50) و d(v,10) ﺗﺮﺗ ﻪﺑ
ﯿ ﺮﻄﻗ ﺐ
ﻂﺳﻮﺘﻣ 90 ، 50 و 10 ﻣ ردﻮﭘ تارذ ﺪﺻرد ﯽ
ﺪﺷﺎﺑ .
2 - 10 - ﺰﺠﺗ ﯾ ﻠﺤﺗ و ﻪ ﯿ رﺎﻣآ ﻞ ي
ﺰﺠﺗ ﯾﻪ و ﻠﺤﺗ ﯿ ﻞ هداد ﺎﻫ ي ﻞﺻﺎﺣ زا رﺎﮐ ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ ﺎﺑ هدﺎﻔﺘﺳا زا
شور ﻟﺎﻧآ ﯿﺰ راو ﯾ ﺲﻧﺎ ) ANOVA (
و ﺎﻘﻣ ﯾ تﺎﺴ ﻣ ﯿ ﮕﻧﺎ ﯿ ﻦ ﻂﺳﻮﺗ
شور ﺪﻨﭼ ﻪﻨﻣاد ا ي ﻦﮑﻧاد ﺎﺑ هدﺎﻔﺘﺳا زا مﺮﻧ راﺰﻓا آ رﺎﻣ ي SPSS
ﻪﺨﺴﻧ 16 رد ﺢﻄﺳ ﻤﻃا ﯿ نﺎﻨ 95 ﺪﺻرد مﺎﺠﻧا ﺪﺷ .
3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ
3 - 1 - ﺳرﺮﺑ ﯽ هرﺎﺼﻋ جاﺮﺨﺘﺳا نﺎﻣﺪﻧار
هرﺎﺼﻋ نﺎﻣﺪﻧار ﮔﯿ
ﺮ ي ﺧ شور ﯿ ﻧاﺮﺤﺑ نودﺎﻣ بآ و نﺪﻧﺎﺴ ﯽ
) رد
نﺎﻣز و ﺎﻫﺎﻣد ﺎﻫ
ي توﺎﻔﺘﻣ ( ﺳرﺮﺑ درﻮﻣ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
ﺎﺘﻧ ﯾ نﺎﺸﻧ ﺞ
ﺧ شور ﻪﮐ داد ﯿ
ﺮﺘﻤﮐ نﺪﻧﺎﺴ ﯾ
ﺛﺎﺗ ﻦ ﯿ جاﺮﺨﺘﺳا رد ار ﺮ زا هرﺎﺼﻋ
ﺖﺳا هدﻮﺑ اراد رﺪﻨﮐ ﻎﻤﺻ .
ﻣ رد ﯿ هرﺎﺼﻋ نﺎ ﺎﻫ ي ﻪﺑ زا هﺪﻣآ ﺖﺳد
ﻧاﺮﺤﺑ نودﺎﻣ بآ شور ﯽ
ﻧ ﯿ ﻪﺑ هرﺎﺼﻋ ﺰ ﺎﻣد زا هﺪﻣآ ﺖﺳد
ي 120
ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز و داﺮﮔ 5
ﻗد ﯿ ﺮﺘﻤﮐ ،ﻪﻘ ﯾ نﺎﻣﺪﻧار راﺪﻘﻣ ﻦ
هرﺎﺼﻋ ﮔﯿ ﺮ ي ﮔ هرﺎﺼﻋ نﺎﻣﺪﻧار ﺎﺑ ﻪﮐ دﻮﺑ اراد ار ﯿﺮ
ي شور
ﺧ ﯿ ﻨﻌﻣ فﻼﺘﺧا ،نﺪﻧﺎﺴ ﯽ
راد ي ﺖﺷاﺪﻧ ) p>0.05 ) ( ﻞﮑﺷ 1 .(
ﺑﯿ ﺮﺘﺸ ﯾ هرﺎﺼﻋ نﺎﻣﺪﻧار راﺪﻘﻣ ﻦ ﮔﯿ
ﺮ ي ﻧ ﯿ شور زا ﻞﺻﺎﺣ هرﺎﺼﻋ ﻪﺑ ﺰ
ﺎﻣد رد SCW ي 160 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز و داﺮﮔ 5
ﻗد ﯿ طﻮﺑﺮﻣ ﻪﻘ
ﻨﻌﻣ فﻼﺘﺧا و دﻮﺑ ﯽ
راد ي ﺎﺳ ﺎﺑ ﯾ هرﺎﺼﻋ ﺮ ﺖﺷاد ﺎﻫ )
p<0.05 .(
اﺮﺷ رد بآ زا هدﺎﻔﺘﺳا ﯾ
ﻧاﺮﺤﺑ نودﺎﻣ ﻂ ﯽ
ﻟد ﻪﺑ ، ﯿ ﺎﻣد ﻞ ي و ﻻﺎﺑ
اﺰﻓا ﯾ رد رﺎﺸﻓ ﺶ ﺣ
ﯿ اﺮﻓ ﻦ ﯾ اﺰﻓا ﺚﻋﺎﺑ ،ﺪﻨ ﯾ
ﺮﺿ ﺶ ﯾ و لﻼﺣ ذﻮﻔﻧ ﺐ
ﻠﺑﺎﻗ ﯿ ا رد هرﺎﺼﻋ جاﺮﺨﺘﺳا ﺖ ﯾ
ﻣ شور ﻦ ﯽ
ددﺮﮔ 22] .[
6ﻮﺋﺎﻫ و
نارﺎﮑﻤﻫ ) 2019 ( ﺑﯿ اﺰﻓا ﺎﺑ ﻪﮐ ﺪﻧدﺮﮐ نﺎ ﯾ
زا ﺎﻣد ﺶ 110
ﺎﺗ 170
ﺘﻧﺎﺳ ﻪﺟرد ﯽ
اﺰﻓا هرﺎﺼﻋ جاﺮﺨﺘﺳا نﺎﻣﺪﻧار ،داﺮﮔ ﯾ
ﺶ ﯾ ﺖﺳا ﻪﺘﻓﺎ
23] .[ ﻟ
7ﯽ نارﺎﮑﻤﻫ و )
2020 ( ﺑﯿ ﺪﻨﺘﺷاد نﺎ اﺰﻓا ﻪﮐ
ﯾ رد ﺎﻣد ﺶ
6Hao
7Li
ﻧاﺮﺤﺑ نودﺎﻣ بآ شور ﯽ
اﺰﻓا ﺚﻋﺎﺑ ﯾ
ﺮﺿ ﺶ ﯾ رد لﻼﺣ ذﻮﻔﻧ ﺐ
ﻣ ﻪﻧﻮﻤﻧ ﯽ ددﺮﮔ .ا ﯾ ﺛﺎﺗ ﻦ ﯿ اﺮﺑ ﺮ ي ﻪﻧﻮﻤﻧ ﺎﻫ ﯾ ﯽ ﺒﺸﺧ ﺖﻓﺎﺑ ﻪﮐ ﯽ
ﺖﻔﺳ و
ﻤﻫا زا ﺪﻧراد ﯿ
ﺑ ﺖ ﯿ ﺮﺘﺸ ي ﺖﺳا رادرﻮﺧﺮﺑ 24]
[
Fig 1 Extraction efficiency of extracts obtained from subcritical water and maceration methods.
Statistical analysis was carried out at α<0.05.
3 - 2 - ﺳرﺮﺑ ﯽ ﻐﺗ ﯿﯿ ﮐﺮﺗ راﺪﻘﻣ تاﺮ ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ
ﻞﮐ
ﻞﮑﺷ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ 3
ﺮﺗﻻﺎﺑ ﯾ راﺪﻘﻣ ﻦ ﻪﺑ هرﺎﺼﻋ رد TPC
هﺪﻣآ ﺖﺳد
ﻧاﺮﺤﺑ نودﺎﻣ بآ زا ﯽ
ﺎﻣد رد ي 160 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز و داﺮﮔ 15
ﻗد ﯿ ﻪﺑ ﻪﻘ ﺎﺳ ﺎﺑ ﻪﮐ ﺪﻣآ ﺖﺳد ﯾ
هرﺎﺼﻋ ﺮ ﺎﻫ ي ﻪﺑ شور رد هﺪﻣآ ﺖﺳد
ﺧ شور و SCW ﯿ
ﻨﻌﻣ فﻼﺘﺧا نﺪﻧﺎﺴ ﯽ
راد ي ﺖﺷاد ) p<0.05 (
) ﻞﮑﺷ 3 .(
دﺎﻘﻣ ﯾ ﮐﺮﺗ ﺮ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ هرﺎﺼﻋ رد رادﻮﻤﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ
ﺳﺮﺗ دراﺪﻧﺎﺘﺳا ﯿ
ﻟﺎﮔ ﺐﺴﺣ ﺮﺑ هﺪﺷ ﻢ ﯿ
ﺳا ﮏ ﯿ ﺬﭘ ترﻮﺻ ﺪ ﯾ
ﻓﺮ ﺖ
) ﻞﮑﺷ 2 .(
اﺰﻓا ﺎﺑ ﯾ شور رد جاﺮﺨﺘﺳا نﺎﻣز و ﺎﻣد ﺶ راﺪﻘﻣ SCW
ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻨﻌﻣ ترﻮﺻ ﻪﺑ ﯽ
راد ي اﺰﻓا ﯾ ﺶ ﯾ ﺖﻓﺎ . ﺮﺘﻤﮐ ﯾ ﻦ
ﮐﺮﺗ راﺪﻘﻣ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻧ ﯿ ﺧ شور زا ﻞﺻﺎﺣ هرﺎﺼﻋ ﻪﺑ ﺰ ﯿ
نﺪﻧﺎﺴ
ﻨﻌﻣ فﻼﺘﺧا ﻪﮐدﻮﺑ طﻮﺑﺮﻣ ﯽ
راد ي رد هﺪﺷ جاﺮﺨﺘﺳا ﻪﻧﻮﻤﻧ ﺎﺑ
شور ) SCW ﺎﻣد ي 120 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز و داﺮﮔ 5
ﻗد ﯿ ﻪﻘ (
ﺖﺷاﺪﻧ ) p>0.05 .(
اﺰﻓا ﺎﺑ ﯾ شور رد ﺎﻣد ﺶ ﻠﺑﺎﻗ ،SCW
ﯿ ﺖ
ﮐﺮﺗ لﻼﺤﻧا ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻧ ﯿ اﺰﻓا ﺰ ﯾ ﻣ ﺶ ﯽ ﯾ ﺪﺑﺎ . اﺰﻓا ﺎﺑ نآ ﺮﺑ هوﻼﻋ ﯾ
ﺶ
و ،ﺎﻣد ﯾ زﻮﮑﺴ ﯾ ﺶﻫﺎﮐ بآ ﻪﺘ ﯾ
ﺛﺎﺗ ﻪﺑ طﻮﺑﺮﻣ ﻪﮐ ﻪﺘﻓﺎ ﯿ
اﺰﻓا ﺖﺒﺜﻣ ﺮ ﯾ
ﺶ
شور رد ﺎﻣد ﻣ SCW
ﯽ ﺖﺣار ذﻮﻔﻧ ﻪﺑ ﺮﺠﻨﻣ و ﺪﺷﺎﺑ ﻪﺑ لﻼﺣ ﺮﺗ
ﻣ ﻪﻧﻮﻤﻧ ﯽ ددﺮﮔ 24] .[ ﺰﻓا اﯾ شور رد جاﺮﺨﺘﺳا نﺎﻣز ﺶ ﻪﺑ SCW
ﺑ سﺎﻤﺗ ﻪﻄﺳاو ﯿ
ﺑ ﺮﺘﺸ ﯿ اﺰﻓا ﻪﺑ ﺮﺠﻨﻣ ﻪﻧﻮﻤﻧ و بآ ﻦ ﯾ
نﺎﻣﺪﻧار ﺶ
ﮐﺮﺗ جاﺮﺨﺘﺳا ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ا ﻪﮐ ﺪﺷ ﯾ ﺛﺎﺗ ﻦ ﯿ اﺰﻓا زا ﺮ ﯾ لﻼﺣ ذﻮﻔﻧ ﺶ
ﺷﺎﻧ ﯽ ﻣ ﯽ ددﺮﮔ 15] .[ جاﺮﺨﺘﺳا شور اﺮﺑ SCW
ي جاﺮﺨﺘﺳا ﮐﺮﺗ
ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ
ﻔﻠﺘﺨﻣ ﻊﺑﺎﻨﻣ زا ﯽ
رﺎﻣزر ﻪﻠﻤﺟ زا ي
25] ،[
ﻮﻧﺎﮔروا 26] ﻨﻫ ،[ ﻪﻧاوﺪ 27] ﻮﻠﻫ و[ 28] ﺖﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ [ .
ﻧاﺮﺤﺑ نودﺎﻣ بآ ﯽ
ﻟد ﻪﺑ ﯿ ﺎﻣد ﻞ ي ﻻﺎﺑ ي اﺮﻓ مﺎﺠﻧا ﯾ ﺮﺿ ،ﺪﻨ ﯾ ذﻮﻔﻧ ﺐ
ﺑﯿ ﺮﺘﺸ ي ﺎﺳ ﻪﺑ ﺖﺒﺴﻧ ﯾ
لﻼﺣ ﺮ ﺎﻫ ي ﺷ ﯿﻤ ﯿﺎﯾ ﯽ دراد . شور رد ﺎﻫ ي
لﻼﺣ زا ﻪﮐ جاﺮﺨﺘﺳا لﻮﻤﻌﻣ ﺎﻫ
ي ﻟآ ﯽ ﻧﻮﺘﺳا ،ناﺰﮕﻫ ﺪﻨﻧﺎﻣ ﯿ
ﺮﺘ ﯾ و ﻞ
...
ﻣ هدﺎﻔﺘﺳا ﯽ ﻠﺑﺎﻗ ،دﻮﺷ ﯿ د زا هدﺎﻔﺘﺳا ﺖ ﺎﻫﺎﻣ
ي و ﻪﺘﺷاﺪﻧ دﻮﺟو ﻻﺎﺑ
اﯾ ﺮﺿ ﺶﻫﺎﮐ ﻪﺑ ﺮﺠﻨﻣ ﺮﻣا ﻦ ﯾ
نﺎﻣﺪﻧار ﺶﻫﺎﮐ و لﻼﺣ ذﻮﻔﻧ ﺐ
ﮐﺮﺗ جاﺮﺨﺘﺳا ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ﻣ ﯽ دﻮﺷ .
Fig 2 Standard curve of gallic acid.
Fig 3 TPC of extracts obtained from subcritical water and maceration methods.
Statistical analysis was carried out at α<0.05.
3 - 3 - ﺳرﺮﺑ ﯽ دار ترﺪﻗ ﯾ
ﮔ لﺎﮑ ﯿ
ﮔﺪﻧﺮ ﯽ
هرﺎﺼﻋ ﺎﻫ ي ﺟاﺮﺨﺘﺳا ﯽ
دار بﺬﺟ ترﺪﻗ ﯾ
لﺎﮑ ﺎﻫ ي هرﺎﺼﻋ ﻂﺳﻮﺗ دازآ ﺎﻫ
ي هﺪﺷ جاﺮﺨﺘﺳا
شور ﻪﺑ ﺧ و SCW
ﯿ ﺳرﺮﺑ درﻮﻣ نﺪﻧﺎﺴ ﯽ
ﺖﻓﺮﮔ راﺮﻗ .
ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﻞﮑﺷ 3 ﻣ ﯽ ﺑ ناﻮﺗ ﯿ ﺧ شور زا ﻞﺻﺎﺣ هرﺎﺼﻋ ﻪﮐ دﻮﻤﻧ نﺎ ﯿ
،نﺪﻧﺎﺴ
ﺮﺘﻤﮐ ﯾ دار بﺬﺟ ترﺪﻗ ﻦ ﯾ
لﺎﮑ ﺎﻫ ي دازآ ﻪﮐ دﻮﺑ اراد ار DPPH
ﻨﻌﻣ ترﻮﺻ ﻪﺑ ﯽ
راد ي ﺎﺳ زا ﯾ هرﺎﺼﻋ ﺮ دﻮﺑ ﺮﺘﻤﮐ ﺎﻫ )
p<0.05 .(
Fig 4 RSA% of extracts obtained from subcritical water and maceration methodscompared to BHA and
ascorbic acid antioxidants.
Statistical analysis was carried out at α<0.05.
ﺑﯿ ﺮﺘﺸ ﯾ دار ترﺪﻗ ﻦ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ ﻧ ﯿ ﺘﻧآ ﻪﺑ ﺰ ﯽ ﺴﮐا ﯿ ﺰﺘﻨﺳ ناﺪ ي
ﻨﻌﻣ ترﻮﺼﺑ ﻪﮐ دﻮﺑ طﻮﺑﺮﻣ BHA ﯽ
راد ي ﺎﺳ زا ﯾ هرﺎﺼﻋ ﺮ ﺎﻫ ي
ﺟاﺮﺨﺘﺳا ﯽ ﺑ ﯿ دﻮﺑ ﺮﺘﺸ ) p<0.05 .(
ﻣ رد ﯿ هرﺎﺼﻋ نﺎ ﺎﻫ ي زا ﻞﺻﺎﺣ
شور هرﺎﺼﻋ ،SCW
ﺎﻫ ي ﺟاﺮﺨﺘﺳا ﯽ ﺎﻣد رد هﺪﺷ ي
160 و 120
ﺘﻧﺎﺳ ﻪﺟرد ﯽ ﺗﺮﺗ ﻪﺑ نﺎﻣز تﺪﻣ رد داﺮﮔ ﯿ
ﺐ 5 و 15 ﻗد ﯿ فﻼﺘﺧا ،ﻪﻘ
ﻨﻌﻣ ﯽ راد ي ﺎﺑ ﯾ ﺪﮑ ﯾ ﺘﻧآ و ﺮﮕ ﯽ ﺴﮐا ﯿ ﺳا ناﺪ ﯿ ﺑرﻮﮑﺳآﺪ ﯿ ﺪﻨﺘﺷاﺪﻧ ﮏ
) p>0.05 .(
دار ترﺪﻗ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ هرﺎﺼﻋ ﺎﻫ ي زا ﻞﺻﺎﺣ
شور ﺑ ﺖﻬﺑﺎﺸﻣ SCW
ﯿ ﺮﺘﺸ ي ﺘﻧآ ﺎﺑ ﯽ ﺴﮐا ﯿ ﺳا ناﺪ ﯿ ﺑرﻮﮑﺳآ ﺪ ﯿ ﮏ
ﺖﺷاد ) ﻞﮑﺷ 4 .(
دار ﯾ لﺎﮑ DPPH ﺮﻃ زا
ﯾ ﻢﺗا اﺪﻫا ﻖ ﺎﻫ
ي
ﻫﯿ ﺘﻧآ ﻂﺳﻮﺗ نژورﺪ ﯽ
ﺴﮐا ﯿ ناﺪ ﻏ ،ﺎﻫ ﯿ ﻣ لﺎﻌﻓﺮ ﯽ و دﻮﺷ ﻪﺑDPPH
هدﺮﺘﺴﮔ رﻮﻃ ا
ي ﻪﺑ زرا رﻮﻈﻨﻣ ﯾﺑﺎ
ﯽ دار ترﺪﻗ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ
ﮐﺮﺗ ﯿ ﺒﻃ تﺎﺒ ﯿﻌ ﯽ ﻣ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ ﯽ
ﮔﯿ دﺮ 29] .[ ﺑ نﺎﻘﻘﺤﻣ ﯿ نﺎ
ا ﺎﺑ ﻪﮐ ﺪﻧدﺮﮐ اﺰﻓ
ﯾ شور رد زا ﺎﻣد ﺶ دار ترﺪﻗ ،SCW
ﯾ لﺎﮑ
ﮔﯿ ﮔﺪﻧﺮ ﯽ هرﺎﺼﻋ ﻧ ﺎﻫ ﯿ اﺰﻓا ﺰ ﯾ ﺶ ﯾ ﺖﺳا ﻪﺘﻓﺎ 23]
.[ آ ي
8ﺎﺳاو و
نارﺎﮑﻤﻫ ) 2019 ( ﺑﯿ اﺰﻓا ﻪﮐ ﺪﻧدﺮﮐ نﺎ ﯾ
شور رد ﺎﻣد ﺶ SCW
ﺮﺨﺗ ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ ﯾ
ﺎﻣد ﺐ ﯾ ﯽ ﮐﺮﺗ ﯿ ﺘﻧآ تﺎﺒ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺶﻫﺎﮐ و
ﺛﺎﺗ ﯿ نآ ﺮ دﻮﺷ ﺮﺠﻨﻣ ﺎﻫ 30]
.ﻟ[
9ﯽ ﻨﭽﻤﻫ نارﺎﮑﻤﻫ و ﯿ
ﺑ ﻦ ﯿ ﺪﻧدﺮﮐ نﺎ
اﺰﻓا ﺎﺑ ﻪﮐ ﯾ دار ترﺪﻗ ،جاﺮﺨﺘﺳا نﺎﻣز ﺶ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ هرﺎﺼﻋ ﺎﻫ
ﻧﯿ اﺰﻓا ﺰ ﯾ ﺶ ﯾ ﺎﺘﻧ ﺎﺑ دﺎﻀﺗ رد ﻪﮐ ﺖﺳا ﻪﺘﻓﺎ ﯾ
ا ﺞ ﯾ ﻘﺤﺗ ﻦ ﯿ ﻖ
ﻣ ﯽ ﺪﺷﺎﺑ 24] .[
8Iwassa 9Li
3 - 4 - ﻌﺗ ﯿﯿ ارﺎﮐ ﺮﺜﮐاﺪﺣ ﺎﺑ هرﺎﺼﻋ ﻦ ﯾ
ﯽ شور رد
ﻧاﺮﺤﺑ نودﺎﻣ بآ ﯽ
نﻮﻣزآ مﺎﺠﻧا زا ﺲﭘ ﺎﻫ
ي ﻌﺗ ﯿﯿ ﺘﻧآ ترﺪﻗ ﻦ ﯽ
ﺴﮐا ﯿ ﻧاﺪ ﯽ راﺪﻘﻣ ،
ﮐﺮﺗ ﯿ ﻟﻮﻨﻓ تﺎﺒ ﯽ ﮐ ﮔ هرﺎﺼﻋ نﺎﻣﺪﻧار و ﻞ ﯿﺮ
ي اﺮﺷ ، ﯾ ﻬﺑ ﻂ ﯿ جاﺮﺨﺘﺳا ﻪﻨ
شور رد دار بﺬﺟ ﺮﺜﮐاﺪﺣ ﻦﺘﻓﺮﮔ ﺮﻈﻧ رد ﺎﺑSCW
ﯾ لﺎﮑ ﺎﻫ ي
ﺑ ،دازآ ﯿ ﺮﺘﺸ ﯾ ﮐﺮﺗ راﺪﻘﻣ ﻦ ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ﺮﺗﻻﺎﺑ و ﯾ ﻣ ﻦ ﯿ نﺎﻣﺪﻧار ناﺰ
ﮔ هرﺎﺼﻋ ﯿﺮ ي ﻌﺗ ، ﯿﯿ ﻪﺑ و ﻦ ﺎﻣزآ ﻪﻣادا رﻮﻈﻨﻣ ﯾ
ﺳرﺮﺑ درﻮﻣ تﺎﺸ ﯽ
ا ﻪﻋﻮﻤﺠﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻪﮐ ﺖﻓﺮﮔ راﺮﻗ ﯾ
ﺼﻋ ،ﺎﻫﺮﺘﻣارﺎﭘ ﻦ ﻞﺻﺎﺣ هرﺎ
ﻧاﺮﺤﺑ نودﺎﻣ بآ شور زا ﯽ
ﺎﻣدرد ي 160 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
و داﺮﮔ
نﺎﻣز 5 ﻗد ﯿ دﺮﮔ بﺎﺨﺘﻧا ،ﻪﻘ ﯾ
ﺪ.
3 - 5 - ﺳرﺮﺑ ﯽ ﺻﺎﺧ ﯿ ﻣﺪﺿ ﺖ ﯿ
ﺑوﺮﮑ ﯽ هرﺎﺼﻋ ﺎﻫ
ي
هﺪﺷ جاﺮﺨﺘﺳا
ﮐﺮﺗ ﯿ ﺷ تﺎﺒ ﯿﻤ ﯿﺎﯾ ﯽ ﮔ ﯿ ﻫﺎ ﯽ ﻔﻠﺘﺨﻣ ﯽ ﺒﻃ ترﻮﺻ ﻪﺑ ﯿﻌ
ﯽ ﮔ رد ﯿ ،نﺎﻫﺎ
ﻪﺑ لﻮﮑﻟﻮﻣ ناﻮﻨﻋ ﺎﻫ
ي ﺑ ﺮﺛﻮﻣ ﯿ ژﻮﻟﻮ ﯾ ﮑ ﯽ ﺣ ﺶﻘﻧ ﻪﮐ دراد دﻮﺟو ، ﯿﺗﺎ
ﯽ
ﮔ رد ﯿﺎ ﻪﺑ و ﻪﺘﺷاد ﻒﻠﺘﺨﻣ نژﻮﺗﺎﭘ ﻞﻣاﻮﻋ ﺮﺑاﺮﺑ رد نﺎﻫ ناﻮﻨﻋ
ﮐﺮﺗ ﯿ ﻣﺪﺿ تﺎﺒ ﯿ
ﺑوﺮﮑ ﯽ و ﺪﺷر ﺶﻫﺎﮐ ﺎﺑ ، ﯾ
ﺑ زا ﺎ ﯿ ا ندﺮﺑ ﻦ ﯾ
ﻦ
ﻣﯿ ﻧﺎﮔرواوﺮﮑ ﯿ ﺴ ﯿ ﻢ ﻣ ﻞﻤﻋ ،ﺎﻫ ﯽ
ﺎﻤﻧ ﯾ ﺪﻨ 31] .[ ازا ﯾ ﻦ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ وور
ﺴﻧﺎﺘﭘ ﯿ ﮔ ﻞ ﯿ ﻪﺑ هدﺎﻔﺘﺳا رد نﺎﻫﺎ ﺎﻫوراد ناﻮﻨﻋ
ي ﻣﺪﺿ ﯿ زا و بوﺮﮑ
ﻓﺮﻃ ﯽ اﺰﻓا ﯾ ﻧ نوﺰﻓا زور ﺶ ﯿ
ﻪﺑ زﺎ ﯾ ﻦﺘﻓﺎ ﺎﺟ ﯾ ﺰﮕ ﯾ ﺒﻃ ﻦ ﯿﻌ ﯽ ﺎﻫوراد ي
ﺷ ﯿﻤ ﯿﺎﯾ ﯽ ﻣﺪﺿ صاﻮﺧ ، ﯿ
ﺑوﺮﮑ ﯽ ﺳرﺮﺑ درﻮﻣ رﺪﻨﮐ ﻎﻤﺻ ﯽ
راﺮﻗ
ﺖﻓﺮﮔ . ترﺪﻗ هرﺎﺼﻋ ﺎﻫ ي ﻞﺻﺎﺣ زا شور ﺎﻫ ي ﻒﻠﺘﺨﻣ جاﺮﺨﺘﺳا
) ﻞﻣﺎﺷ هرﺎﺼﻋ ﮔﯿ ﺮ ي ﻪﺑ شور ﺧ ﯿ و نﺪﻧﺎﺴ هرﺎﺼﻋ
ﮔ ﯿﺮ ي ﺎﺑ بآ
نودﺎﻣ ﻧاﺮﺤﺑ ﯽ (،
رد ﺛﺎﺗ ﯿﺮ ﺮﺘﮐﺎﺑﺮﺑ ي ﺎﻫ ي مﺮﮔﻮﺘﺒﺜﻤﻣﺮﮔ ﻔﻨﻣ
ﯽ درﻮﻣ
زرا ﯾﺑﺎ ﯽ راﺮﻗ ﺖﻓﺮﮔ . اﺮﺑ ي اﯾ ﻦ رﻮﻈﻨﻣ زا ﺮﺘﮐﺎﺑ ي اﯾ ﺷﺮﺸ ﯿ ﻠﮐﺎ ﯽ
ﻪﺑ ﺮﺘﮐﺎﺑ ناﻮﻨﻋ ي
مﺮﮔ ﻔﻨﻣ ﯽ و زا ﺮﺘﮐﺎﺑ ي ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ
،سﻮﺋروا
ﻪﺑ ﺮﺘﮐﺎﺑ ناﻮﻨﻋ ي
مﺮﮔ ﺖﺒﺜﻣ هﺮﻬﺑ ﻪﺘﻓﺮﮔ ﺪﺷ . ﺎﻔﮐ ﯾ ﺖ ﻞﻤﻋ و تﺪﺷ
ﺛﺎﺗ ﯿ راﺬﮔﺮ ي هرﺎﺼﻋ ﺎﻫ ﺮﺑ اﯾ ﻦ ود ﻪﺘﺳد زا ﺮﺘﮐﺎﺑ ي ﺎﻫ ﻪﺑ ﺗﺮﺗ ﯿ ﺐ ﺎﺑ
هدﺎﻔﺘﺳا زا فﺮﻌﻣ ﺮﺗ ي ﻨﻓﯿ ﻞ ﻟوزاﺮﺘﺗ ﯿ مﻮ اﺮﻠﮐ ﯾ ﺪ و شور ﺶﻧاﻮﺧ
بﺬﺟ ﺤﻣ ﯿ ﻂ ﺖﺸﮐ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﻂﺳﻮﺗ ﻻا ﯾ راﺰ ﯾ رﺪ ترﻮﺻ ﺖﻓﺮﮔ .
ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ ﻞﮑﺷ 5 ﻣ ﯽ ناﻮﺗ ﺑﯿ نﺎ دﻮﻤﻧ ﻪﮐ هرﺎﺼﻋ ﺎﻫ ي ﻞﺻﺎﺣ زا
شور ﺎﻫ ي بآ نودﺎﻣ ﻧاﺮﺤﺑ ﯽ
، ﺖﺒﺴﻧ ﻪﺑ هرﺎﺼﻋ ﻞﺻﺎﺣ زا شور
ﺧ ﯿ
،نﺪﻧﺎﺴ ﺛﺎﺗ ﯿﺮ ﺑﯿ ﺮﺘﺸ ي رد ﺶﻫﺎﮐ ﺪﺷر ﺮﺘﮐﺎﺑ ي اﯾ ﺷﺮﺸ ﯿ ﻠﮐﺎ ﯽ
ﺪﻨﺘﺷاد . ﻪﺘﮑﻧ ﻞﺑﺎﻗ ﻪﺟﻮﺗ رد اﯾ ﻦ
،ﺶﺨﺑ هﻮﺤﻧ و تﺪﺷ ﺛﺎﺗ ﯿ راﺬﮔﺮ ي
هرﺎﺼﻋ ﺎﻫ ﺮﺑ ﺪﺷر ﺮﺘﮐﺎﺑ ي ﺎﻫ ي درﻮﻣ ﺳرﺮﺑ ﯽ
، ﺎﺑ ﻪﺟﻮﺗ ﻪﺑ عﻮﻧ
ﺶﺷﻮﭘ د ﯾ هراﻮ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ نآ ﺎﻫ ﻣ ﯽ ﺪﺷﺎﺑ . ﺎﺑ ﺎﻘﻣ ﯾ ﻪﺴ بﺬﺟ ﺎﻫ ي ﻪﺑ
هﺪﻣآ ﺖﺳد زا
هﺎﮕﺘﺳد ﻻا ﯾ راﺰ ﯾ رﺪ ﺺﺨﺸﻣ دﺮﮔ
ﯾﺪ ﻪﮐ ﺛﺎﺗ ﯿﺮ
هرﺎﺼﻋ ﺎﻫ ي ﻞﺻﺎﺣ زا شور ﺎﻫ ي ﺧ ﯿ بآ و نﺪﻧﺎﺴ نودﺎﻣ
ﻧاﺮﺤﺑ ﯽ
،
ﺮﺑ ﺮﺘﮐﺎﺑ ي ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ
،سﻮﺋروا ﺑﯿ ﺮﺘﺸ زا ﺮﺘﮐﺎﺑ ي اﯾ ﺷﺮﺸ ﯿ ﻠﮐﺎ ﯽ
هدﻮﺑ ﺖﺳا . ﺗرﺎﺒﻋ ﻪﺑ ﯽ ﺮﺘﮐﺎﺑ ي ﻓﺎﺘﺳا ﯿ ﺳﺎﺴﺣ ،سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﯿ
ﺖ
ﺑﯿ ﺮﺘﺸ ي هرﺎﺼﻋ ﺮﺑاﺮﺑ رد ﺎﻫ
ي ﺟاﺮﺨﺘﺳا ﯽ ﺖﺷاد رﺪﻨﮐ ﻎﻤﺻ زا .ا
ﯾ ﻦ
ﺘﻧﯿ ﻣ نﺎﺸﻧ ﻪﺠ ﯽ
ﺛﺎﺗ ﻪﮐ ﺪﻫد ﯿ
شور زا ﻞﺻﺎﺣ هرﺎﺼﻋ ﺮ زا SCW
ﺮﺘﮐﺎﺑ ﺮﺑ ،رﺪﻨﮐ ﻎﻤﺻ ي
ﺎﻫ ي ﺑ ﺖﺒﺜﻣ مﺮﮔ ﯿ
ﺖﺳا هدﻮﺑ ﺮﺘﺸ .ا
ﯾ ﺘﻧ ﻦ ﯿ ﻪﺠ
ﻣ ار ﯽ د رﺎﺘﺧﺎﺳ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ناﻮﺗ ﯾ
ﻟﻮﻠﺳ هراﻮ ﯽ ﺟﻮﺗ ﯿ دﻮﻤﻧ ﻪ . زﯾ اﺮ
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ اراد ي ﺎﺸﻏ ي ﺟرﺎﺧ ﯿ ﻟ ﺲﻨﺟ زﺎ ﯿ
ﻠﭘﻮﭙ ﯽ
رﺎﮐﺎﺳ ﯾ ﺎﻘﻣ رد ﻪﮐ ﺖﺳا ﺎﻫﺪ ﯾ
ﺮﺘﮐﺎﺑ ﺎﺑ ﻪﺴ ي
ﺎﻫ ي ﻌﻧﺎﻣ ﺖﺒﺜﻣ مﺮﮔ ﯽ
ﻓﺎﺿا ﯽ ﺖﺳا 32] .[
ﺖﻈﻠﻏ ﺎﻫ ﯾ ﯽ زا هرﺎﺼﻋ ﻪﮐ اراد ي ﺮﺛا ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﺪﺷر
،ﺪﻧدﻮﺑ
ﻪﺑ ﺳرﺮﺑ رﻮﻈﻨﻣ ﯽ
رﺪﻗ ت ﺮﺘﮐﺎﺑ ي ﺸﮐ ﯽ
، رد نﻮﻣزآ ﻪﻧﺎﺨﻣﺮﮔ راﺬﮔ ي
درﻮﻣ ﺳرﺮﺑ ﯽ ﺪﻨﺘﻓﺮﮔراﺮﻗ )
لوﺪﺟ .(
ﺪﺑ ﯾ ﻦ ترﻮﺻ ﻪﮐ ﺖﻈﻠﻏ ﺎﻫ ي
ﺮﺗﻻﺎﺑ زا ﺖﻈﻠﻏ اراد ي ﺮﺛا ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﻪﺑ هاﺮﻤﻫ ﺖﻈﻠﻏ اراد ي ﺮﺛا
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﻪﺑ ﺤﻣ ﯿ ﻂ ﺖﺸﮐ ﺪﺷر ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ هدوﺰﻓا وﺪﺷ ﺮﺘﮐﺎﺑ
ي ﺎﻫ ي اﯾ ﺷﺮﺸ ﯿ ﻠﮐﺎ ﯽ و ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ سﻮﺋروا
ﺮﺑ ور ي
ﺤﻣ ﯿ ﻂ ﮐ
،ﺖﺸ ﺖﺸﮐ هداد ﺪﻧﺪﺷ . ﺎﺘﻧ ﯾ ﺞ ﮐﺎﺣ ﯽ زا نآ دﻮﺑ ﻪﮐ
ﺖﻈﻠﻏ 200000ppm
زا ﻣﺎﻤﺗ ﯽ هرﺎﺼﻋ ﺎﻫ ي جاﺮﺨﺘﺳا
،هﺪﺷ اراد ي
ﺛﺎﺗ ﯿﺮ ﺮﺘﮐﺎﺑ ي ﺸﮐ ﯽ هدﻮﺑ ﺖﺳا .
3 - 6 - ﻌﺗ ﯿﯿ ژﺮﭙﺳآ ﺪﺿ ترﺪﻗ ﻦ ﯾ
ﺳﻮﻠ ﯽ ردﻮﭘ
رﯾ ﻧﺎﺷﻮﭘﺰ ﯽ هﺪﺷ
ﻬﺑ هرﺎﺼﻋ ﯿ ﺧ شور زا ﻞﺻﺎﺣ ﻪﻨ ﯿ
و نﺪﻧﺎﺴ ردﻮﭘ ﻪﺑ SCW
رﯾ ﻧﺎﺷﻮﭘﺰ ﯽ ﺪﺒﺗ هﺪﺷ ﯾ دﺮﮔ ﻞ ﯾ ﺪ . ﺎﺘﻧ ﯾ ﮐﺎﺣ ﺞ ﯽ ا ﻪﮐ دﻮﺑ نآ ز
ﺖﻄﻠﻏ ﺎﻫ ي 300 و 600 راﺰﻫ ﺪﻗﺎﻓ ،رﺪﻨﮐ ﻎﻤﺻ هرﺎﺼﻋ زا ppm
ژﺮﭙﺳآﺪﺿ ﺮﺛا ﯾ
ﺳﻮﻠ ﯽ ﺖﺳا هدﻮﺑ .
ﺲﻧﺎﺳا ﺎﻫ ي ﮔﯿ ﻫﺎ ﯽ زا ﺖﺧﺎﺳ و
زﺎﺳ
،DNA
،RNA ﺌﺗوﺮﭘ ﯿ ﻦ ﺎﻫ و ﻠﭘ ﯽ رﺎﮐﺎﺳ ﯾ ﺎﻫﺪ رد لﻮﻠﺳ ﺎﻫ ي
ﭼرﺎﻗ ﯽ و ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﮔﻮﻠﺟ ﯿﺮ ي ﻣ ﯽ ﺎﻤﻧ ﯾ ﺪ 33] .[ ﻠﻋ ﯽ هدازﺪﻤﺤﻣ و
نارﺎﮑﻤﻫ ) 1399 ( ﺑﯿ نﺎ ﺪﻧدﺮﮐ ﻪﮐ ﺎﺼﻋ هر ﻞﺻﺎﺣ زا ز ﯾ هﺮ ﺰﺒﺳ رد
ﺖﻈﻠﻏ ﺎﻫ ي ﺮﺗﻻﺎﺑ زا 6000 ppm
زا ﺪﺷر ﮏﭙﮐ ژﺮﭙﺳآ ﯾ سﻮﻠ
سووﻼﻓ و ﻟﻮﺗ ﯿ ﺪ ﺴﮐﻮﺗﻼﻓآ ﯿ ﻦ ﮔﻮﻠﺟ ﯿﺮ ي ﻣ ﯽ ﺎﻤﻧ ﯾ ﺪ 34] .[
Fig 5 MIC concentration in Maceration and SCW extracts for E-coli and S.aureus.
Statistical analysis has not been performed for this parameter.
Table 1 Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Maceration and SCW extracts for E-coli and S.aureus.
MIC concentration (ppm) MBC concentration (ppm) Extraction Method Staphylococcus
aureus Escherichia coli Staphylococcus
aureus Escherichia coli
Maceration 100000 100000 200000 200000
SCW 160 0C- 5min 25000 50000 200000 200000
* Statistical analysis has not been performed for this parameter
3 - 7 - هزاﺪﻧا زاﺪﻧا يﺮﯿﮔ
ه ﺴﻟﻮﻣا تارذ و نﻮﯿ
ﺺﺧﺎﺷ ﯽﮔﺪﯿﺷﺎﭘ ﺲﺑ
ر ردﻮﭘ تﺎﺼﺨﺸﻣ ﯾ
ﻧﺎﺷﻮﭘﺰ ﯽ ﻬﺗ ،رﺪﻨﮐ ﻎﻤﺻ هﺪﺷ ﯿ
شور ﻪﺑ هﺪﺷ ﻪ
ﺸﺷﺎﭘ ﻦﮐ ﮏﺸﺧ ﯽ
لوﺪﺟ رد 2
ﺖﺳا هﺪﺷ ﻪﺋارا .
ﺳرﺮﺑ ﺎﺑ ﯽ ﻞﮑﺷ
ﻨﺤﻨﻣ ﯽ ﺎﻫ ي زﻮﺗ ﯾ زﻮﺗ ﻪﮐ ﺪﺷ ﺺﺨﺸﻣ تارذ هزاﺪﻧا ﻊ ﯾ
هزاﺪﻧا ﻊ
ﻬﺗ ردﻮﭘ رد تارذ ﯿ
اراد هﺪﺷ ﻪ ي
زﻮﺗ ﯾ لادﻮﻣﻮﻧﻮﻣ عﻮﻧ زا و لﺎﻣﺮﻧ ﻊ
) Monomodal (
ﺖﺳا هدﻮﺑ .
ﻓﺮﻃ زا ﯽ اﺰﻓا ﺎﺑ ﯾ راﺪﻬﮕﻧ نﺎﻣز ﺶ ي
ﻨﺤﻨﻣ ،ردﻮﭘ ﯽ
ﺎﻫ ي زﻮﺗ ﯾ ﻐﺗ ﺖﺳار ﺖﻤﺳ ﻪﺑ تارذ هزاﺪﻧا ﻊ ﯿﯿ
نﺎﮑﻣ ﺮ
ﺪﻧداد . ﺗرﺎﺒﻋ ﻪﺑ ﯽ گرﺰﺑ ردﻮﭘ تارذ هزاﺪﻧا ﺮﻄﻗ ﻂﺳﻮﺘﻣ و ﺪﺷ ﺮﺗ
ﺷﺎﭘ ﺲﺑ ﺺﺧﺎﺷ و تاﺮﻄﻗ ﯿ
ﮔﺪ ﯽ اﺰﻓا تارذ ﯾ
ﺶ ﯾ ﺖﻓﺎ ) لوﺪﺟ 2 .(
ﻠﮐ رﻮﻃ ﻪﺑ ﯽ
اﺰﻓا ﯾ ﺮﻄﻗ ﺶ ﻂﺳﻮﺘﻣ ﻃ رد ردﻮﭘ تارذ ﯽ
يراﺪﻬﮕﻧ
د نﺎﺸﻧ ﺪﭘ عﻮﻗو هﺪﻨﻫ ﯾ
هﺪ يﺎﻫ ﺎﭘﺎﻧ ﯾ ﻢﻫ ﻪﺑ و ﻊﻤﺠﺗ ﻪﻠﻤﺟ زا يراﺪ
ﭘﯿ ﻦﺘﺳﻮ ﺖﺳا تارذ 35]
.[ ارﺎﮐ رد رد تارذ هزاﺪﻧا ﯾ
ﯽ ﻠﮐ ﯽ ﯾ ﮏ
ﻪﺑ ردﻮﭘ ﺸﺨﺑ ناﻮﻨﻋ ﯽ
ﺳ زا ﯿ ﻮﺤﺗ ﻢﺘﺴ ﯾ اراد لﺎﻘﺘﻧا و ﻞ ي
ﻤﻫا ﯿ ﺖ
ﻣ ﯽ ﺪﺷﺎﺑ 36] .[ زا ﺮﺘﻤﮐ ﻪﮐ ردﻮﭘ تارذ هزاﺪﻧا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ 100
ﻣ ﺮﺘﻣﻮﻧﺎﻧ ﯽ ﻬﺗ ردﻮﭘ ،ﺪﺷﺎﺑ ﯿ
هﺮﺘﺴﮔ رد هﺪﺷ ﻪ ﺪﻨﺑ ﻪﻘﺒﻃ ردﻮﭘﻮﻧﺎﻧ
ي
ﻣ ﯽ ددﺮﮔ 22] .[
Table 2 Characteristics of coated frankincense powder.
Time (day)
Size Distribution (µm)
Mean diameter (volume-long)
Mean diameter (surface-weight)
SpecificSurface m2/ml
D10 D50 D90 D 4,3 D 3,2 SSA
Span
0 4.1 10.6 35.4 20.59 15.88 0.377834 2.95283
24 6.3 18.9 84.93 44.36 15.42 0.389105 4.160317
* Statistical analysis has not been performed for this parameter
4 - ﺘﻧ ﯿ ﮔ ﻪﺠ ﯿﺮ ي ﻠﮐ ﯽ
اﯾ ﻧاﺮﺤﺑ نودﺎﻣ بآ جاﺮﺨﺘﺳا شور زا ﻪﻌﻟﺎﻄﻣ ﻦ ﯽ
اﺮﺑ ي
هرﺎﺼﻋ ﮔﯿ ﺮ ي ﺎﺘﻧ و ﺪﺷ هدﺎﻔﺘﺳا رﺪﻨﮐ ﻎﻤﺻ زا ﯾ
ﺘﻨﺳ شور ﺎﺑ نآ ﺞ ﯽ
ﺧ ﯿ ﺎﻘﻣ درﻮﻣ نﺪﻧﺎﺴ ﯾ
ﺖﻓﺮﮔ راﺮﻗ ﻪﺴ .
ﺎﺘﻧ ﯾ ﮐﺎﺣ ﺞ ﯽ ﻪﮐ دﻮﺑ نآ زا
شور ﺛﺎﺗ SCW ﯿ اﺰﺴﺑ ﺮ ﯾ ﯽ ﮔ هرﺎﺼﻋ نﺎﻣﺪﻧار راﺪﻘﻣ رد ﯿﺮ
ي ﻪﺘﺷاد
ﺖﺳا . ﻨﭽﻤﻫ ﯿ دﺮﮔ ﺺﺨﺸﻣ ﻦ ﯾ
شور دﺮﺑرﺎﮐ ﺎﺑ ﺪ اﺮﺑ SCW
ي
ﮔ هرﺎﺼﻋ ﯿﺮ ي ﮐﺮﺗ راﺪﻘﻣ ،رﺪﻨﮐ ﻎﻤﺻ زا ﯿ
ﻟﻮﻨﻓ تﺎﺒ ﯽ ﺪﺷ جاﺮﺨﺘﺳا ه
ﻧﯿ ﻨﻌﻣ ترﻮﺻ ﻪﺑ ﺰ ﯽ
راد ي اﺰﻓا ﯾ ﺶ ﯾ ﺖﺳا ﻪﺘﻓﺎ . ﺛﺎﺗ ﯿ شور ﺖﺒﺜﻣ ﺮ
ﺎﺘﻧ رد SCW ﯾ دار بﺬﺟ ترﺪﻗ ﺞ ﯾ
لﺎﮑ ﺎﻫ ي دازآ ﻧ ﯿDPPH ﺰ
هرﺎﺼﻋ ﺪﺷ ﺚﻋﺎﺑ و دﻮﺑ دﻮﻬﺸﻣ ﺎﻫ
ﯾ ﯽ دار ترﺪﻗ ﺎﺑ ﯾ
ﮔ لﺎﮑ ﯿ ﮔﺪﻧﺮ ﯽ
ﺑﯿ ﺮﺘﺸ ي دﻮﺷ ﻞﺻﺎﺣ .
شور زا ﻞﺻﺎﺣ هرﺎﺼﻋ ترﺪﻗ SCW
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﯽ ﺮﺘﮐﺎﺑ ﺪﺷر زا ي
ﺮﺗﻻﺎﺑ ي ور زا ﻞﺻﺎﺣ هرﺎﺼﻋ زا ش
ﺧ ﯿ ﺖﺷاد نﺪﻧﺎﺴ .
ﺛﺎﺗ ﯿ هرﺎﺼﻋ ﺮ ﺎﻫ ي ﺮﺑ رﺪﻨﮐ ﻎﻤﺻ زا ﻞﺻﺎﺣ ﺮﺘﮐﺎﺑ
ي ﺎﻫ ي ﺑ ﺖﺒﺜﻣ مﺮﮔ ﯿ
ﺛﺎﺗ زا ﺮﺘﺸ ﯿ ﺮﺘﮐﺎﺑ ﺮﺑ نآ ﺮ ي
ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ
دﻮﺑ . ﻨﭽﻤﻫ ﯿ دﺮﮔ ﺺﺨﺸﻣ ﻦ ﯾ
ﻪﺑ رﺪﻨﮐ ﻎﻤﺻ هرﺎﺼﻋ ﺪ زا هﺪﻣآ ﺖﺳد
ﺎﻣد ي 160 ﺘﻧﺎﺳ ﻪﺟرد ﯽ
نﺎﻣز و داﺮﮔ 5
ﻗد ﯿ ﺖﻈﻠﻏ رد ،ﻪﻘ ﺎﻫ
ي
زا ﺮﺗﻻﺎﺑ 600 ﭘ راﺰﻫ ﯽ ﭘ ﯽ اراد ما ي ﺛا ژﺮﭙﺳآﺪﺿ ﺮ ﯾ
ﺳﻮﻠ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ .
ﺎﺘﻧ ﯾ ﺞ هزاﺪﻧا ﮔﯿ ﺮ ي زﻮﺗ ﯾ ﻊ هزاﺪﻧا تارذ نﺎﺸﻧ داد ﻪﮐ هزاﺪﻧا تارذ
اراد ي زﻮﺗ ﯾ ﻊ لﺎﻣﺮﻧ دﻮﺑ . ﺎﺑ ﺳرﺮﺑ ﯽ ﺛﺎﺗ ﯿﺮ ﺮﺘﻣارﺎﭘ نﺎﻣز رد راﺪﻬﮕﻧ ي
ردﻮﭘ ﻟﻮﺗ ﯿ ﺪ هﺪﺷ ﺺﺨﺸﻣ دﺮﮔ ﯾ ﺪ ﻪﮐ اﺰﻓا ﯾ ﺶ نﺎﻣز رﺎﮔﺪﻧﺎﻣ ي ردﻮﭘ
ﺚﻋﺎﺑ اﺰﻓا ﯾ ﺶ ﺺﺧﺎﺷ ﺲﺑ ﺷﺎﭘ ﯿ ﮔﺪ ﯽ تارذ هﺪﺷ ﺖﺳا .
5 - ﻊﺑﺎﻨﻣ
[1] Seku, K., Hussaini, S.S., Hussain, M., Siddiqui, M.A., Golla, N., Ravinder, D., Reddy, B. 2022. Synthesis of Frankincense gum stabilized AgNPs by microwave irradiation and their catalytic, antioxidant, and
antibacterial properties. Physica E: Low- dimensional Systems and Nanostructures, 140:
115169.
https://doi.org/10.1016/j.physe.2022.115169.
[2] Umar, S., Umar, K., Sarwar, A. H. M. G., Khan, A., Ahmad, N., Ahmad, S. 2014.
Boswellia serrata extract attenuates inflammatory mediators and oxidative stress in collagen induced arthritis. Phytomedicine, 21(6): 847–856.
[3] Shahidpour, F., Zare Mehrjerdi, F., Mozayan, M.R., Marefati, N., Hosseini, M.
2021. The effects of frankincense extract on depression and anxiety-like behaviors induced by lipopolysaccharide in rats. Learning and
Motivation, 73: 101708.
https://doi.org/10.1016/j.lmot.2021.101708 [4] Kheirkhah, H., Baroutian, S., Quek, S.Y.
2019. Evaluation of bioactive compounds extracted from Hayward kiwifruitpomace by subcritical water extraction. Food Bioprod.
Process.,115: 143–153.
[5] Kora, A.L., Rastogi, L. 2018. Green synthesis of palladium nanoparticles using gum ghatti (Anogeissus latifolia) and its application as an antioxidant and catalyst.
Arabian Journal of Chemistry, 11: 1097-1106.
[6] Weber, C.C., Reising, K., Müller, W. E., Schubert-Zsilavecz, M., Abdel-Tawab, M.
2006. Modulation of Pgp function by boswellic acids. Planta Medica Journal, 72 (06), 507–
513.
[7] Peng, S., Song, Z., Wang, C., Liang, D., Wan, X., Liu, Z., Lu, A., Ning, Z. 2022.
Frankincense vinegar-processing improves the absorption of boswellic acids by regulating bile acid metabolism. Phytomedicine 98:
153931.
https://doi.org/10.1016/j.phymed.2022.153931 [8] Wang, Y., Ye, Y., Wang, L., Yin, W., Liang,
J. 2021. Antioxidant activity and subcritical water extraction of anthocyanin from raspberry process optimization by response surface methodology. Food Bioscience, 44: 101394.
https://doi.org/10.1016/j.fbio.2021.101394 [9] Nastic, N., Svarc-Gajic, J., Delerue-Matos,
C., Morais, S., Barroso, M.F., Moreiram M.M.
2018. Subcritical water extraction of antioxidants from mountain germander (Teucrium montanum L.). The Journal of Supercritical Fluids, 138: 200-206.
[10] Svarc-Gajić, J., Stojanović, Z., Carretero, A.S., Román, D.A., Borrás, I., Vasiljević, I.
2018. Development of a microwave-assisted extraction for the analysis of phenolic compounds from Rosmarinus officinalis, J.
Food Eng. 119: 525–532.
[11] Liang, X., Fan, Q. 2013. Application of sub-critical water extraction in pharmaceutical industry. J. Mater. Sci. Chem.Eng. 1, 1.
[12] Todd, R., Baroutian, S. 2017. A techno- economic comparison of subcritical water, supercritical CO2 and organic solvent extraction of bioactives from grape marc. J.
Clean. Prod. 158,349–358.
[13] Guthrie, F., Wang, Y., Neeve, N., Quek, S.Y., Mohammadi, K., Baroutian, S. 2020.
Recovery of phenolic antioxidants from green kiwi fruit peel using subcritical water extraction. Food and Bioproducts Processing, 122: 136-144.
[14] Rodrigues, A.L., Matias, A.A., Paiva, A.
2021. Recovery of antioxidant protein hydrolysates from shellfish waste streams using subcritical water extraction. Food and Bioproducts Processing, 130: 154-163.
[15] Shaddel, R., Maskooki, A., Haddad- Khodaparast, M.H., Azadmard-Damirchi, S., Mohamadi, M., Fathi-Achachlouei, B. 2014.
Optimization of Extraction Process of Bioactive Compounds from Bene Hull Using Subcritical Water. Food Science and biotechnology, 23 (5): 1459-1468.
[16] Asl, A.H., M.J.M.T.-A.I.S.E. Khajenoori, Modeling, E.O.N. 2013.Subcritical Water Extraction., pp. 459–487.
[17] Abdelmoez, W., Nage, S.M., Bastawess, A., Ihab, A., Yoshida, H.J. 2014. Subcritical water technology for wheat straw hydrolysis to produce value added products. J. Clean. Prod.
70, 68–77.
[18] Song, R., Ismail, M., Baroutian, S., Farid, M. 2018. Effect of subcritical water on the extraction of bioactive compounds from carrot leaves. Food Bioprocess 11, 1895–1903.
[19] Murugkar, D. A., Zanwar, A.A., Shrivastava, A. 2021. Effect of nano- encapsulation of flaxseed oil on the stability, characterization and incorporation on the quality of eggless cake. Applied Food
Research, 1: 100025.
https://doi.org/10.1016/j.afres.2021.100025.
[20] Mohammadi, M., Ghorbani, M., Beigbabaei, A., Yeganehzad, S., Sadeghi- Mahoonak, A. 2019. Investigation effects of extracted compounds from shell and cluster of
pistachio nut on the inactivation of free
radicals. Heliyon, 5: DOI:
https://doi.org/10.1016/j.heliyon.2019.e02438.
[21] Ghazanfari, N., Mortazavi, S.A., Tabatabaei Yazdi, F., Mohammadi, M. 2020. Microwave- assisted hydrodistillation extraction of essential oil from coriander seeds and evaluation of their composition, antioxidant and antimicrobial activity. Heliyon, 6 (9):
DOI:
https://doi.org/10.1016/j.heliyon.2020.e04893 [22] Gandon-Ros, G., Soler, A., Aracil, I.,
Gomez-Rico, M.F., Conesa, J.A. 2021.
Improving efficiency and feasibility of subcritical water debromination of printed circuit boards E-waste via potassium carbonate adding. Journal of Cleaner Production, 319:
128605,
https://doi.org/10.1016/j.jclepro.2021.128605.
[23] Hao, G., Cao, W., Lia, T., Chen, J., Zhang, J., Weng, W., Osako, K., Ren, H. 2019. Effect of temperature on chemical properties and antioxidant activities of abalone viscera subcritical water extract. The Journal of Supercritical Fluids, 147: 17-23.
https://doi.org/10.1016/j.supflu.2019.02.007.
[24] Li, B., Akram, M., Al-Zuhair, S., Elnajjar, E., Munir, M.T. 2020. Subcritical water extraction of phenolics, antioxidants and dietary fibres from waste date pits. Journal of Environmental Chemical Engineering, 8:
104490.
https://doi.org/10.1016/j.jece.2020.104490.
[25] Basile, A., Jim´enez-Carmona, M.M., Clifford, A.A. 1998. Extraction of rosemary by superheated water, J. Agric. Food Chem. 46 (12) (1998) 5205–5209.
[26] Rodríguez-Meizoso, I., Marin, F.R., Herrero, M., Senorans, F.J., Reglero, G., Cifuentes, A., Ibanez, E. 2006. Subcritical water extraction of nutraceuticals with antioxidant activity from oregano. Chemical and functional characterization, J. Pharm.
Biomed. Anal. 41 (5): 1560–1565.
[27] Kim, S.J., Matsushita, Y., Fukushima, K., Aoki, D., Yagami, S., Yuk, H.G., Lee, S.C.
2014. Antioxidant activity of a hydrothermal extract from watermelons, LWT-Food Sci.
Technol., 59 (1): 361–368.
[28] Giombelli, C., Iwassa, I.J., da Silva, C., Bolanho Barros, B.C. 2020. Valorization of peach palm by-product through subcritical water extraction of soluble sugars and phenolic
compounds, J. Supercrit. Fluids, 165, 104985.
[29] Bougatef, A., Hajji, M., Balti, R., Lassoued, I., Triki-Ellouz, Y., Nasri, M. 2009.
Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteases, Food Chem. 114 (2009) 1198–1205, https://doi.org/10.1016/j.foodchem.2008.10.07 5.
[30] Iwassa, I.J., dos Santos Ribeiro, M.A., Meurer, E.C., Cardozo-Filho, L., Bolanho, B.C., da Silva, C. 2019. Effect of subcritical water processing on the extraction of compounds, composition, and functional properties of asparagus by-product, J. Food Process Eng. 42 (4): e13060.
[31] Tariq, A.L., Reyaz, A.L. 2012.
Significances and importance of phytochemical present in Terminalia chebula.
Intern. J. Drug Develop. Res. 5 (3), 256–262.
[32] Acimovic, M., Seregelj, V., Sovljanski , O., Saponjac , V.T., Gajic, J.S., Brezo-Borjan, T., Pezo, L. 2021. In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. Subcritical water extract.
Industrial Crops & Products 169: 113672.
https://doi.org/10.1016/j.indcrop.2021.11367.
[33] Bakkali, f., Daomar, D. Averbeck, S. and Averbeck, B. 2008. Biological effects of essential oils- A review. Food Chem. Toxicol.
46: 446-457.
[34] Ali Mohammad Zadeh, M., Ali Doust, M., Khandaghi, J. 2020. A study of antimicrobial effects of alcoholic extract and essential oil of caraway (Bunium persicum Boiss) on selected species of bacteria and molds in lactic cheese.
Journal of Food Microbiology, 7 (4): 33-46.
[35] Tadros, T., Izquierdo, R., Esquena, J., Solans, C. 2004. Formation and stability of nano-emulsions. Advances in Colloid and Interface Science, 303–318.
[36] Kouassi, G. K. , Teriveedhi, V. K. , Milby, C. L. , Ahmad, T. , Boley, M. S., Gowda, N.
M., Terry, R. J. 2012. Nano- microencapsulation and controlled release of linoleic acid in biopolymer matrices: Effects of the physical state, water activity, and quercetin on oxidative stability. Journal of Encapsulation and Adsorption Sciences, 02 (01), 1–10.
Application of subcritical water method in extraction from frankincense gum and evaluation of antioxidant and antibacterial
ability of extracts.
Golchin, S. 1, Hajirostamloo, B. 1*, Didar, Z. 1,Mohsen Vazifedoost1, Mohammadi, M. 2
1. Department of Food Science and Technology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
2. Department of Food Processing, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.
ABSTRACT ARTICLE INFO
Article History:
Received 2022/ 07/ 19 Accepted 2022/ 10/ 18
Keywords:
Subcritical water, Aspergillus flavus, Frankincense gum, Radical scavenging ability, Lacto bacillus.
Extraction by the subcritical water method was studied as an efficient, economical, green, and environmentally friendly method to extract effective compounds of frankincense gum.For this purpose, subcritical water was used at temperatures of 120 and 160 ° C for 5 and 15 minutes. The amount of total phenolic compounds, radical scavenging ability, and antimicrobial properties of the extracts were investigated. The encapsulated powder of the extract obtained from the optimal conditions was also prepared by spray drying method and the particle size distribution and anti-aspergillus properties of that were also investigated. The results showed that the subcritical water extraction method significantly increased the extraction efficiency of frankincense gum extract.
The amount of phenolic compounds extracted at 160 °C and time of 15 minutes was the highest among the extracts with 130.83 mg of gallic acid per 100g dry matter. The radical scavenging ability of the extracts from the subcritical water method was similar to that of the natural antioxidant ascorbic acid, and the extract from the maceration method as a traditional was less efficient at absorbing DPPH free radicals.Microbial results showed that the effects of extracts from maceration and subcritical water methods on Staphylococcus aureus were more than Escherichia coli. Encapsulated frankincense powder had no anti-aspergillus potency at concentrations less than 600,000 μg / ml.
DOI: 10.22034/FSCT.19.130.143 DOR: 20.1001.1.20088787.1401.19.130.13.2
*Corresponding Author E-Mail:
Journal of Food Science and Technology (Iran)
Homepage:www.fsct.modares.ir Scientific Research
