ﻪﺠﻧﻮﻳ ﻲﻜﻴﺘﻧژ يراﺪﻳﺎﭘ ﻲﺳرﺮﺑ ﻪﺘﺨﻳراﺮﺗ يﺎﻫ
ا ﺎﺑ Bt ﺮﮕﻧﺎﺸﻧ زا هدﺎﻔﺘﺳ RAPD
ﻢﻳﺮﻣهﺪﻴﺳ يرﺎﺨﺘﻓا
٭
ﺮﻓﺪﻴﺣﻮﺗدﻮﻌﺴﻣ،1
ﻲﺟﺎﻧﺪﻤﺤﻣﺮﻴﻣا،2
ﺞﻴﺋﺎﻧدﺮﻛﻦﻳﺪﻟاءﻼﻋ،1
ﻲﻤﻳﺮﻛﺮﺑﺎﺟ،1
1
ﺪﻫﺎﺷهﺎﮕﺸﻧادتﺎﺗﺎﺒﻧحﻼﺻاويژﻮﻟﻮﻨﻜﺗﻮﻴﺑهوﺮﮔ1
ناﺮﻳايزروﺎﺸﻛيژﻮﻟﻮﻨﻜﺗﻮﻴﺑهﺪﻜﺸﻫوﮋﭘ2
ﻪﺠﻧﻮﻳ ﻲﻜﻳ زا نﺎﻫﺎﻴﮔ ﻪﻓﻮﻠﻋ يا ﻢﻬﻣ ﺖﺳا يﺪﻳﺪﺷترﺎﺴﺧﻪﻟﺎﺳﺮﻫﻪﺠﻧﻮﻳگﺮﺑﻲﻣﻮﻃﺮﺧﺮﺳ . ﻲﻣدراوﻪﺠﻧﻮﻳعراﺰﻣﻪﺑ
ﺪﻨﻛ وﺖﻓآﻦﻳاترﺎﺴﺧزايﺮﻴﮔﻮﻠﺟياﺮﺑ .
نژ يوﺎﺣ ﻪﺘﺨﻳراﺮﺗ ﻪﺠﻧﻮﻳ هﺎﻴﮔ ،مﻮﻤﺳ فﺮﺼﻣ ﺶﻫﺎﻛ cry3A
يﺮﺘﻛﺎﺑ زا ﻪﻛ Bacillus.turengensis.var.tenebrionis)
و ﺪﺷ ﺪﻴﻟﻮﺗ ،ﻪﺘﺸﮔ اﺪﺟ (
دﺮﻛﺪﻴﻳﺎﺗهﺎﻴﮔﻦﻳاردارنژرﻮﻀﺣﻲﻟﻮﻜﻟﻮﻣ يﺎﻫﺰﻴﻟﺎﻧآ ﺶﻬﺟدﻮﺟوﻪﺘﺨﻳراﺮﺗنﺎﻫﺎﻴﮔﺪﻴﻟﻮﺗﺎﺑﻪﻄﺑاررددﻮﺟﻮﻣيﺎﻫﻲﻧاﺮﮕﻧزاﻲﻜﻳ .
وﻲﻣوزﻮﻣوﺮﻛتﺎﻓاﺮﺤﻧاوﺎﻫ
ﻲﻣنﺎﻫﺎﻴﮔﻦﻳاﻲﻳاززﺎﺑوﺖﻓﺎﺑﺖﺸﻛردﻲﻟﺎﻧﻮﻠﻛﺎﻣﻮﺳتاﺮﻴﻴﻐﺗ ﺪﺷﺎﺑ
ﺑﻖﻴﻘﺤﺗﻦﻳازافﺪﻫ. ﻪﺠﻧﻮﻳﻲﻜﻴﺘﻧژﻲﺘﺧاﻮﻨﻜﻳﻲﺳرﺮ
نژهﺪﻨﻨﻛنﺎﻴﺑﻪﺘﺨﻳراﺮﺗيﺎﻫ cry3A
ﻪﺠﻧﻮﻳﺎﺑﻪﺴﻳﺎﻘﻣرد ﻲﻟﻮﻜﻟﻮﻣﺮﮕﻧﺎﺸﻧزاهدﺎﻔﺘﺳاﺎﺑﺪﻫﺎﺷهﺎﻴﮔﺎﺑﻪﺘﺨﻳراﺮﺗﻪﺠﻧﻮﻳﻲﻜﻴﺘﻧژﺖﻫﺎﺒﺷناﺰﻴﻣﻦﻴﻴﻌﺗوﻪﺘﺨﻳراﺮﺗﺮﻴﻏﻲﻣﻮﺑي
ﺖﺳاRAPD رﻮﻈﻨﻣﻦﻳﺪﺑ .
اﺪﺘﺑا شورﺎﺑﺪﻫﺎﺷوﻪﺘﺨﻳراﺮﺗهﺎﻴﮔودﺮﻫزاﻲﻣﻮﻧژDNA
ﺎﺑﺲﭙﺳوﺪﺷجاﺮﺨﺘﺳاCTAB ﻲﻓدﺎﺼﺗﺮﻤﻳاﺮﭘ9
RAPD
،ﺖﻓﺮﮔراﺮﻗﻲﺑﺎﻳزرادرﻮﻣوﺪﺷﺮﻴﺜﻜﺗ
ﻲﺑﺎﻳزرا ﺖﻫﺎﺒﺷﺎﻫ ﺎﺗ95
ﺷﺪﻴﻳﺎﺗﻪﺘﺨﻳراﺮﺗنﺎﻫﺎﻴﮔردﻲﻜﻴﺘﻧژﺮﻴﻐﻐﺗﻪﻧﻮﮔﺮﻫوﺶﻬﺟدﻮﺟومﺪﻋودادنﺎﺸﻧﺪﻫﺎﺷﻪﺑﺖﺒﺴﻧارﻪﺘﺨﻳراﺮﺗهﺎﻴﮔيﺪﺻرد100 ﺪ
.
ﻠﻛ نﺎﮔژاو ﻴ
ﺪ ي :
يراﺪﻳﺎﭘ
،ﻪﺘﺨﻳراﺮﺗﻪﺠﻧﻮﻳ،ﻲﻜﻴﺘﻧژ RAPD
ﻪﻣﺪﻘﻣ
گﺮﺑﻲﻣﻮﻃﺮﺧﺮﺳ Hypera postica)
دﻮﺑرﻮﺸﻛردﻪﺠﻧﻮﻳﻢﻬﻣتﺎﻓآزا ( ﻦﻴﺑزارﻮﺸﻛﻖﻃﺎﻨﻣﺮﺜﻛاردارﻪﺠﻧﻮﻳلواﻦﻴﭼ،ﺖﺳردﺎﻧهزرﺎﺒﻣﻪﻄﺳاوﻪﺑًﻻﻮﻤﻌﻣﻪﻛه
ﻲﻣ دﺮﺑ دوﺪﺣ ﻪﻧﻻﺎﺳﻦﻴﮕﻧﺎﻴﻣرﻮﻃ ﻪﺑ. شزراﻪﺑيزروﺎﺸﻛمﻮﻤﺳ ﻦﺗ7000
دوﺪﺣﻪﻛدﻮﺷ ﻲﻣرﻮﺸﻛدراورﻻد نﻮﻴﻠﻴﻣ 90 ﺎﺗ700
ﻪﺑطﻮﺑﺮﻣﺮﺘﻴﻟ راﺰﻫ900
ﺪﺷﺎﺑﻲﻣﻪﺠﻧﻮﻳﻲﻣﻮﻃﺮﺧﺮﺳ )
8 ياﺮﺑ .(
ايﺮﻴﮔﻮﻠﺟ نژيوﺎﺣﻪﺘﺨﻳراﺮﺗﻪﺠﻧﻮﻳهﺎﻴﮔ،مﻮﻤﺳفﺮﺼﻣﺶﻫﺎﻛوﺖﻓآﻦﻳاترﺎﺴﺧز
cry3A يﺮﺘﻛﺎﺑزاﻪﻛ Bacillus.
turengensis.var. tenebrionis ) ﺣ ﻲﻟﻮﻜﻟﻮﻣيﺎﻫﺰﻴﻟﺎﻧآوﺪﺷﺪﻴﻟﻮﺗ،ﻪﺘﺸﮔاﺪﺟ(
دﺮﻛﺪﻴﻳﺎﺗهﺎﻴﮔﻦﻳاردارنژرﻮﻀ )
15 ( ﺺﺨﺸﻣتﺎﻌﻟﺎﻄﻣزايرﺎﻴﺴﺑرد .
ﻟﺎﻛءﺎﻘﻟاوﻲﻳاززﺎﺑﻪﻛﺖﺳاهﺪﺷ ﻜﻤﻣﺖﺸﻛﻂﻴﺤﻣردسﻮ
زﻮﻣوﺮﻛتﺎﻓاﺮﺤﻧادﺎﺠﻳاﺚﻋﺎﺑﺖﺳاﻦ ﻲﻜﻴﺘﻧژتاﺮﻴﻴﻐﺗوﻲﻣو
ﺮﮔ دد ) 11 لﺎﺜﻣناﻮﻨﻋ ﻪﺑ .(
ﻪﻌﻟﺎﻄﻣرد يا
ﮔﻪﻛﺖﺳاهﺪﺷﺺﺨﺸﻣ ﻮﺗوﺮﭘﻖﻳﺮﻃزاهﺪﺷﻲﻳاززﺎﺑنﺎﻫﺎﻴ
هﺪﺷاززﺎﺑنﺎﻫﺎﻴﮔﻦﻳاﻦﻴﺑﻲﻗﻼﺗزاﻞﺻﺎﺣيﺎﻫﺪﻳﺮﺒﻴﻫﻦﻴﻨﭽﻤﻫوﺖﺳﻼﭘ
، 50 ﻲﻌﻴﺒﻃﺮﻴﻏموزﻮﻣوﺮﻛ %
ﺪﻧاﻪﺘﺷاد ﻋ .
ﻪﻛﺖﺳاهﺪﺷشراﺰﮔﻦﻳاﺮﺑهوﻼ ويرﺎﺘﺧﺎﺳعﻮﻨﺗ
يدﺪﻋ ﻲﻣموزﻮﻣوﺮﻛ ﺪﻧاﻮﺗ
،ﺖﻓﺎﺑﺖﺸﻛتﺪﻣلﻮﻃزاﻲﺷﺎﻧ ﻮﻨﺗ
،ﻲﭙﻴﺗﻮﻧژع عﻮﻧﺎﻳوﺖﻓﺎﺑﺖﺸﻛعﻮﻧ
ﻂﻴﺤﻣ ﺖﺸﻛﻦﺳﻲﺘﺣوﺖﺸﻛ ﺪﺷﺎﺑ
) 9 ﻚﻴﻨﻜﺗ.(
ﺪﻨﻧﺎﻣﻲﻟﻮﻜﻟﻮﻣيﺎﻫ ﻲﻣﻪﻛﺖﺳادﺎﻤﺘﻋاﻞﺑﺎﻗوﻊﻳﺮﺳشورﻚﻳRAPD
اﺮﻴﻴﻐﺗﻲﻬﺟﻮﺗﻞﺑﺎﻗترﻮﺻﻪﺑﺪﻧاﻮﺗ ت
ﺪﻨﻛﺺﺨﺸﻣارﻪﺘﺨﻳراﺮﺗنﺎﻫﺎﻴﮔردﻲﻜﻴﺘﻧژ )
13 ( ﻪﺑ. يﺮﻴﮔرﺎﻛ يﺎﻫﺮﮕﻧﺎﺸﻧ ﻲﻟﻮﻜﻟﻮﻣ
رد ﻪﺠﻧﻮﻳ دﺮﺑرﺎﻛ دﺮﺘﺴﮔ يا ﻪﺘﺷاد
،ﺖﺳا رد ﻦﻳا ﺎﺘﺳار زا يﺎﻫﺮﻛرﺎﻣ ﻲﻟﻮﻜﻟﻮﻣ
ياﺮﺑ ﻲﻳﺎﺳﺎﻨﺷ و ﻪﺴﻳﺎﻘﻣ ﺶﻳارآ ﻲﻜﻴﺘﻧژ مﺎﻗرا و ﺖﻴﻌﻤﺟ يﺎﻫ ﻪﺠﻧﻮﻳ )
،1 4
،( ﻲﻳﺎﺳﺎﻨﺷ ﻲﮔﮋﻳو يﺎﻫ ﺮﺼﺤﻨﻣ ﻪﺑ دﺮﻓ مرژ ﻢﺳﻼﭘ ﻧﻮﻳ ﻪﺠ ) 10
،( ﻪﺸﻘﻧ ﻲﺑﺎﻳ ﻲﻟﻮﻜﻟﻮﻣ
ﺎﻜﻣ ن يﺎﻫ لﺮﺘﻨﻛ هﺪﻨﻨﻛ تﺎﻔﺻ ﻢﻬﻣ ﺮﺑ يور مﻮﻧژ ﻪﺠﻧﻮﻳ )
،12
،14 5
،( ﻲﻳﺎﺳﺎﻨﺷ ژ يﺎﻫ لﺎﻘﺘﻧا ﻪﺘﻓﺎﻳ ﻪﺑ جﺎﺘﻧ رد ﻲﻗﻼﺗ ﻦﻴﺑ نﻮﮔ يﺎﻫ ﻪﺠﻧﻮﻳ )
،6 7
،( ﻲﺳرﺮﺑ
قﺮﻔﺗ ﻲﻜﻴﺘﻧژ ) 3 ﻪﺑ( رﺎﻛ ﻪﺘﻓر ﺖﺳا ندﺮﻛﺺﺨﺸﻣﻪﻟﺎﻘﻣﻦﻳا زافﺪﻫ . يراﺪﻳﺎﭘ
ﻪﺠﻧﻮﻳ ﻲﻜﻴﺘﻧژ راﺮﺗيﺎﻫ
نژيوﺎﺣﻪﺘﺨﻳ cry3A
ﺪﺷﺎﺑﻲﻣ ﺎﺑ رﻮﻈﻨﻣﻦﻳا ياﺮﺑ .
ﻲﻟﻮﻜﻟﻮﻣﺮﮕﻧﺎﺸﻧزاهدﺎﻔﺘﺳا ﺪﻨﺘﻓﺮﮔراﺮﻗﻲﺑﺎﻳزرادرﻮﻣﻪﺘﺨﻳراﺮﺗيﺎﻫﻪﺠﻧﻮﻳRAPD
.
داﻮﻣ و شور ﺎﻫ
جاﺮﺨﺘﺳا زاﻲﻣﻮﻧژDNA
ﻴﮔ8 هﺎ ﻪﺘﺨﻳراﺮﺗ و ﻲﻔﻨﻣﺪﻫﺎﺷناﻮﻨﻋﻪﺑﻪﺘﺨﻳراﺮﺗﺮﻴﻏهﺎﻴﮔ2 شورﻪﺑ
CTAB ) 2 ﺖﻴﻤﻛوﺖﻴﻔﻴﻛ،ﺖﻓﺮﻳﺬﭘمﺎﺠﻧا ( زاﻞﺻﺎﺣDNA
زرﺎﮔآلژزرﻮﻓوﺮﺘﻜﻟاﻖﻳﺮﻃ ﺖﺸﮔﻦﻴﻴﻌﺗ
.
RAPD PCR :
ﺮﻤﻳاﺮﭘ9 هﺪﺷهدرواﺮﻳزلوﺪﺟردﻪﻛRAPD
هدﺎﻔﺘﺳادرﻮﻣﺮﻳزﻪﻣﺎﻧﺮﺑﻖﺒﻃﺖﺳا ﺖﻓﺮﮔراﺮﻗ
هﺮﻴﺠﻧزﺶﻨﻛاو . ﻲﻠﭘيا
ﻣ ﻲﻳﺎﻬﻧﻢﺠﺣردزاﺮ 20
ﺮﺘﻴﻟوﺮﻜﻴﻣ يﺎﻣد ردﻪﻣﺎﻧﺮﺑﻖﺒﻃ
تﺪﻣ ﻪﺑداﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد 94 ردو ﺪﺷ ﺖﺷﺮﺳاو ﻪﻘﻴﻗد 4
يﺎﻫﺎﻣد ﺎﺑ ﻪﺧﺮﭼ35 )
داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد 94
،ﻪﻘﻴﻗد 1 ﻪﺟرد 42
داﺮﮕﻴﺘﻧﺎﺳ
،ﻪﻘﻴﻗد 1 داﺮﮕﻴﺘﻧﺎﺳﻪﺟرد72
ﻪﻘﻴﻗد1 ردوﺪﺷﺮﻴﺜﻜﺗ( داﺮﮕﻴﺘﻧﺎﺳﻪﺟرد72
هﺎﮕﺘﺳدردﻲﻳﺎﻬﻧﻂﺴﺑﻪﻘﻴﻗد5 ﺮﻠﻜﻳﺎﺳﻮﻣﺮﺗ
Bio-Rad لﺪﻣ
ALS
ﺪﺷمﺎﺠﻧا1296 ﺲﭙﺳ .
لﻮﺼﺤﻣ زرﺎﮔآلژزرﻮﻓوﺮﺘﻜﻟاﻖﻳﺮﻃزا PCR
5 / 1 ﺪﺷﻚﻴﻜﻔﺗ % زﺎﻴﺘﻣا.
يﺪﻨﺑ ﺎﻫﺪﻧﺎﺑ ترﻮﺼﺑ ﺮﻔﺻ مﺪﻋ) دﻮﺟو ﺪﻧﺎﺑ ﻚﻳو ( دﻮﺟو) ﺪﻧﺎﺑ (
ترﻮﺻ ﺖﻓﺮﮔ و. ﺖﻫﺎﺒﺷناﺰﻴﻣ ﺖﺸﮔﻪﺒﺳﺎﺤﻣدرﺎﻛﺎﺟﻪﺑﺎﺸﺗﺐﻳﺮﺿزاهدﺎﻔﺘﺳاﺎﺑ
.
ﺞﻳﺎﺘﻧ و ﺚﺤﺑ
ﺎﺟاﻪﻜﻳرﻮﻃﻪﺑﺖﺳاﻪﺘﻓﺎﻳﻪﻌﺳﻮﺗﻲﻜﻴﺘﻧژتاﺮﻴﻴﻐﺗاﺮﻴﺧا نژﻚﻳﺎﺗﺪﻫدﻲﻣهز
وﻪﺘﺸﮔيزﺎﺳاﺪﺟوﻲﻳﺎﺳﺎﻨﺷصﺎﺧ ﻲـﮔﮋﻳويﺮـﺳﻚـﻳﺎﺑيﺮﮕﻳددﻮﺟﻮﻣﻪﺑ
يﺎـﻫ
ﺪﺳﺮﺑﻲﺑﻮﻠﻄﻣﺪﺣﻪﺑﺖﻴﻔﻴﻛﺎﺗدﻮﺷﻞﻘﺘﻨﻣصﺎﺧ تﻻﻮﺼﺤﻣهﻮﻘﻟﺎﺑيﺎﻳاﺰﻣزا.
ﻪﻳﺬﻐﺗشزراﺶﻳاﺰﻓاGM ﻣﻦﻳايا
ﻲـﮔﮋﻳوندﺮـﺑﻻﺎـﺑﻦﻴﻨﭽﻤﻫوتﻻﻮﺼﺤ _
يﺎـﻫ
ﻳاﻲﺘﻣوﺎﻘﻣ يرﺎﻤﻴﺑوتﺎﻓآﻪﺑﺖﺒﺴﻧتﻻﻮﺼﺤﻣﻦ
ﺎﻫ_ ﻲﻣ ﺪﺷﺎﺑ زﺎﺑونژندادراﺮﻗﻪﻛﺖﺳاﻦﻳانآودراددﻮﺟوﻲﻧاﺮﮕﻧﻚﻳﺎﻣا. ﺖﺳاﻦﻜﻤﻣنآﻲﻳاز
وﺶﻬﺟﺚﻋﺎﺑ
ﻲﻣوژﻮﻣوﺮﻛتﺎﻓاﺮﺤﻧا عﻮﻨﺗ)
ﻲﻟﺎﻧﻮﻠﻛﺎﻣﻮﺳ ﻲﻧاﺮﮕﻧﺚﻋﺎﺑوددﺮﮔ (
ﻲﻨﻤﻳاﺚﺤﺑردﻲﻳﺎﻫ نژتاﺮﻴﺛﺎﺗو
نژﺮﻳﺎﺳﺮﺑهﺪﺷﻞﻘﺘﻨﻣيﺎﻫ ﻤﻧدﺎﺠﻳاارﺎﻫ
ﺪﻳﺎ . ﻖـﻴﻘﺤﺗﻦﻳارد 9
عﻮﻤﺠﻣردﻲﺑﺎﺨﺘﻧاﺮﻤﻳاﺮﭘ ﺗﻪﻛﺪﺷهﺪﻫﺎﺸﻣﺪﻧﺎﺑ610
ﺎﻬﻨ ﺖﺸﮔهﺪﻫﺎﺸﻣﻞﻜﺷﺪﻨﭼﺪﻧﺎﺑ28 زاﺎﻫﺪﻧﺎﺑهزاﺪﻧا .
100 ﺎـﺗ 3000
دﻮـﺑيﺮـﻴﮔهزاﺪـﻧاﻞـﺑﺎﻗزﺎـﺑﺖـﻔﺟ .
ناﺰﻴﻣﻦﻳﺮﺘﻤﻛ ﺮﻤﻳاﺮﭘﻪﺑطﻮﺑﺮﻣﺪﻧﺎﺑداﺪﻌﺗ
opr-14 ﻧﺎﺑناﺰﻴﻣﻦﻳﺮﺘﺸﻴﺑو ﺪ
ﺮﻤﻳاﺮﭘﻪﺑطﻮﺑﺮﻣ opr-07
ﺪﺷﺎﺑﻲﻣ ﻪـﺑطﻮـﺑﺮﻣﻢـﻫفرﻮـﻣﻲـﻠﭘﺪﻧﺎﺑناﺰﻴﻣﻦﻳﺮﺘﺸﻴﺑ
ﺮﻤﻳاﺮﭘ opr-20 ﺪﺷﺎﺑﻲﻣ ﺪﻫﺎﺷهﺎﻴﮔوﻪﺘﺨﻳراﺮﺗهﺎﻴﮔﻦﻴﺑﺖﻫﺎﺒﺷناﺰﻴﻣﺖﻳﺎﻬﻧرد. ﺎﺗ95
ﻪﻛﺖﺳاهﺪﺷﻪﺒﺳﺎﺤﻣﺪﺻرد100 ﺞﻳﺎﺘﻧﻦﻳا
ﺮـﻴﻴﻐﺗمﺪـﻋهﺪﻨﻫدنﺎﺸﻧ
ﺪﺷﺎﺑﻲﻣﻪﺘﺨﻳراﺮﺗيﺎﻫﻪﺠﻧﻮﻳردﻲﻟﺎﻧﻮﻠﻛﺎﻣﻮﺳعﻮﻨﺗ وﻲﻜﻴﺘﻧژ
.
لوﺪﺟ 1 ﺖﻫﺎﺒﺷناﺰﻴﻣ- ﻪﺘﺨﻳراﺮﺗﺮﻴﻏنﺎﻫﺎﻴﮔﺎﺑﻪﺘﺨﻳراﺮﺗنﺎﻫﺎﻴﮔ
ﻪﺘﺨﻳراﺮﺗ 7 ﻪﺘﺨﻳراﺮﺗ 6 ﻪﺘﺨﻳراﺮﺗ 5 ﻪﺘﺨﻳراﺮﺗ 4 ﻪﺘﺨﻳراﺮﺗ 3 ﻪﺘﺨﻳراﺮﺗ 2 ﻪﺘﺨﻳراﺮﺗ 1 ﺪﻫﺎﺷ 2 ﺪﻫﺎﺷ 1 ﭗﻴﺗﻮﻧژ
100
% ﺪﻫﺎﺷ 1
100
% 5 / 97
% ﺪﻫﺎﺷ 2
100
% 5 / 97
% 5 / 97
% ﻪﺘﺨﻳراﺮﺗ 1
100
% 5 / 92
% 5 / 92
% 5 / 92
% ﻪﺘﺨﻳراﺮﺗ 2
100
% 9 / 94
% 5 / 97
% 5 / 97
% 5 / 97
% ﻪﺘﺨﻳراﺮﺗ 3
100
% 100
% 9 / 94
% 5 / 97
% 5 / 97
% 5 / 97
% ﻪﺘﺨﻳراﺮﺗ 4
100
% 7 / 98
% 7 / 98
% 6 / 93
% 7 / 98
% 2 / 96
% 7 / 98
% ﻪﺘﺨﻳراﺮﺗ 5
100
% 7 / 98
% 100
% 100
% 9 / 94
% 5 / 97
% 5 / 97
% 5 / 97
% ﻪﺘﺨﻳراﺮﺗ 6
100
% 2 / 96
% 9 / 94
% 2 / 96
% 2 / 96
% 1 / 91
% 7 / 93
% 8 / 93
% 7 / 93
% ﻳراﺮﺗ ﻪﺘﺨ 7
7 / 98
% 0 / 95
% 7 / 93
% 0 / 95
% 0 / 95
% 0 / 90
% 0 / 95
% 06 / 95
% 5 / 92
% ﻪﺘﺨﻳراﺮﺗ 8
لوﺪﺟ 2 ﺎﻫﺮﻤﻳاﺮﭘﻲﻟاﻮﺗومﺎﻧﺖﺴﻴﻟ -
ﺮﻤﻳاﺮﭘمﺎﻧ ﺮﻤﻳاﺮﭘﻲﻟاﻮﺗ
ﺪﻧﺎﺑداﺪﻌﺗ ﻢﺴﻴﻓرﻮﻣﻲﻠﭘﺪﺻرد
OPN-02 ACCAGGGGCA
90 44
/ 4
%
OPR-07 ACTGGCCTCA
140 0
/ 0
%
OPR-12 ACAGGTGCGT
80 0
/ 0
%
OPR-14 CAGGATTCCC
30 0
/ 0
%
OPR-16 CTCTGCGCGT
60 33
/ 18
%
OPR-09 TGAGCACGAG
70 0
/ 0
%
OPN-10 ACAACTGGGG
70 5
/ 8
%
OPR-04 CCCGTAGCAC
110 0
/ 0
%
OPR-20 ACGGCAAGGA
160 37
/ 4
%
هرﺎﻤﺷﻞﻜﺷ 1
ﺮﻤﻳاﺮﭘ - OPR-12
ﻊﺑﺎﻨﻣ
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