ﺖﻔﺟ جاﺮﺨﺘﺳا ﺪﯾﺪﺟ شور دﺮﺑرﺎﮐ ﺶﯾﺎﭘ ياﺮﺑ ﻻﺎﺑ ﯽﯾارﺎﮐ ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ ﻪﺑ هﺪﺷ
ﯽﺘﻧآ ﺗﻮﯿﺑ ﮏﯿ وﺎﮔ ﺮﯿﺷ رد ﯽﻨﯿﻠﮑﯾﺎﺳاﺮﺘﺗ يﺎﻫ
ﯽﺛار ﻆﻓﺎﺣ ،1
مﺪﻘﻣرﺎﺸﻓا ﺎﺿرﺪﻤﺤﻣ
2 و
،3
ﯽﻗﺪﻨﺧ ﻞﯿﻠﺟ
4
*
1 - ﺶﻧاد ﯽﺳﺎﻨﺷرﺎﮐ ﻪﺘﺧﻮﻣآ ناﺮﯾا ،باﺮﺳ ، ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،باﺮﺳ ﺪﺣاو ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ ﺪﺷرا
.
2 - ناﺮﯾا ،ﺰﯾﺮﺒﺗ ،ﺰﯾﺮﺒﺗ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﯾوراد ﺰﯿﻟﺎﻧآ تﺎﻘﯿﻘﺤﺗ ﺰﮐﺮﻣ رﺎﯾدﺎﺘﺳا .
3 - رﺎﯾدﺎﺘﺳا ناﺮﯾا ،ﺰﯾﺮﺒﺗ ،ﺰﯾﺮﺒﺗ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،وراد و اﺬﻏ ﯽﻨﻤﯾا ﺰﮐﺮﻣ .
4 - ناﺮﯾا ،باﺮﺳ ، ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،باﺮﺳ ﺪﺣاو ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ رﺎﯾدﺎﺘﺳا .
ﻪﻟﺎﻘﻣ تﺎﻋﻼﻃا هﺪﯿﮑﭼ
ﻪﻟﺎﻘﻣ يﺎﻫ ﺦﯾرﺎﺗ :
ﺖﻓﺎﯾرد ﺦﯾرﺎﺗ :
29 / 07 / 99
ﭘ ﺦﯾرﺎﺗ شﺮﯾﺬ : 17 / 12 / 99
يﺪﯿﻠﮐ تﺎﻤﻠﮐ :
،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ
،ﺮﯿﺷ ،ﯽﺸﺨﭘ ﺪﻣﺎﺟ زﺎﻓ جاﺮﺨﺘﺳاوﺮﮑﯿﻣ
ﻻﺎﺑ ﯽﯾارﺎﮐ ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ .
DOI: 10.52547/fsct.18.04.27
تﺎﺒﺗﺎﮑﻣ لﻮﺌﺴﻣ
:
ﯽﺘﻧآ زا ﻪﯾور ﯽﺑ هدﺎﻔﺘﺳا ﮏﯿﺗﻮﯿﺑ
هﺪﻧﺎﻤﯿﻗﺎﺑ ﻊﻤﺠﺗ ﺐﺒﺳ ماد شروﺮﭘ ﺖﻌﻨﺻ رد ﺎﻫ ﯽﺘﻧآ يﺎﻫ
رد ﯽﮑﯿﺗﻮﯿﺑ
ﺖﺷﻮﮔ ﺪﻨﻧﺎﻣ ﻒﻠﺘﺨﻣ ﯽﻣاد تﻻﻮﺼﺤﻣ ﺪﻫاﻮﺧ غﺮﻣ ﻢﺨﺗ و ﺮﯿﺷ ،
ﺪﺷ . ﯽﺘﻧآ يوﺎﺣ ﯽﯾاﺬﻏ داﻮﻣ فﺮﺼﻣ ﮏﯿﺗﻮﯿﺑ
ﺎﻫ
ﺖﻣوﺎﻘﻣ ﺶﯾاﺰﻓا ﺪﻨﻧﺎﻣ ﯽﺗﻼﮑﺸﻣ ﯽﺘﻧآ يﺎﻫ
يژﺮﻟآ دﺎﺠﯾا و ﯽﮑﯿﺗﻮﯿﺑ ﻪﺑ ار
لﺎﺒﻧد دراد . ﺶﯾﺎﭘ ﻞﯿﻟد ﻦﯿﻤﻫ ﻪﺑ
ﯽﺘﻧآ هﺪﻧﺎﻤﯿﻗﺎﺑ ﮏﯿﺗﻮﯿﺑ
ﺖﯿﻤﻫا زا تﺎﯿﻨﺒﻟ و ﺮﯿﺷ ﺪﻨﻧﺎﻣ ﺪﻨﻣدﻮﺳ و فﺮﺼﻣﺮﭘ ﯽﯾاﺬﻏ تﻻﻮﺼﺤﻣ رد صﻮﺼﺨﺑ ﺎﻫ
هﮋﯾو ﺖﺳا رادرﻮﺧﺮﺑ يا .
جاﺮﺨﺘﺳا ياﺮﺑ ﯽﺸﺨﭘ ﺪﻣﺎﺟ زﺎﻓ جاﺮﺨﺘﺳاوﺮﮑﯿﻣ شور ﯽﺸﻫوﮋﭘ رﺎﮐ ﻦﯾا رد
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ ﻠﮑﯾﺎﺳاﺮﺘﺗ ﯽﺴﮐا ،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ يﺎﻫ ﯽﺴﮐاد و ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗﺮﻠﮐ ،ﻦﯿ
زا ﻦﯿﻠﮑﯾﺎﺳ 50
ﻪﻧﻮﻤﻧ و ﺮﯿﺷ
هزاﺪﻧا ﻪﺑ ﺎﻬﻧآ ﯽﻤﮐ يﺮﯿﮔ هداد ﻪﻌﺳﻮﺗ ﻻﺎﺑ ﯽﯾارﺎﮐ ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ شور
ﺪﺷ . ﺮﺛا رﻮﻈﻨﻣ ﻦﯾا ياﺮﺑ
ترﺪﻗ ،بذﺎﺟ راﺪﻘﻣ و عﻮﻧ ،هﺪﻨﻫد بﻮﺳر ﻞﻣﺎﻋ ﻢﺠﺣ ﺪﻨﻧﺎﻣ جاﺮﺨﺘﺳا ﯽﯾارﺎﮐ رد ﺮﺛﻮﻣ ﻒﻠﺘﺨﻣ يﺎﻫﺮﺘﻣارﺎﭘ ﯽﺑد و نﺎﻣز ،ﯽﻧﻮﯾ لﻼﺣ ﻢﺠﺣ و عﻮﻧ و ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﻢﺠﺣ و عﻮﻧ ،اﻮﻫ نﺪﯿﻣد
ﻪﻨﯿﻬﺑ هﺪﻨﯾﻮﺷ ﺪﺷ يزﺎﺳ
. ﺞﯾﺎﺘﻧ
ﻪﻧﻮﻤﻧ ﻪﻤﻫ ﻪﮐ داد نﺎﺸﻧ ﻖﯿﻘﺤﺗ هدوﺪﺤﻣ رد نآ راﺪﻘﻣ و هدﻮﺑ ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ هﺪﻧﺎﻤﯿﻗﺎﺑ ياراد ﺮﯿﺷ يﺎﻫ
146 ﺎﺗ
319 ﯽﻠﯿﻣ رد مﺮﮔﻮﻧﺎﻧ ﺖﺳاﺮﯿﻐﺘﻣ ﺮﺘﯿﻟ
. ﯽﺘﻧآ ﺮﮕﯾد ﮏﯿﺗﻮﯿﺑ
ﻪﻧﻮﻤﻧ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ ﺎﯾ ﺎﻫ
ﺪﺸﻧ ﺖﻓ . يﺎﯾاﺰﻣ زا
ﯽﻣ يدﺎﻬﻨﺸﯿﭘ جاﺮﺨﺘﺳا شور ﻻﺎﺑ ﺖﯿﺳﺎﺴﺣ ﺎﺑ ﺎﻫ ﺖﯿﻟﺎﻧآ طﻮﻠﺨﻣ ﺰﯿﻟﺎﻧآ نﺎﮑﻣا و ﻻﺎﺑ يزﺎﺳاﺪﺟ ترﺪﻗ ﻪﺑ ناﻮﺗ
ﻪﺑ دﺮﮐ هرﺎﺷا يدﺎﻬﻨﺸﯿﭘ شور ﯽﯾارﺎﮐ ،ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﺖﺤﺗ ﻪﮑﯾرﻮﻃ
91 - 80 رد ﺺﯿﺨﺸﺗ دوﺪﺣ و ﺪﺻرد
هدوﺪﺤﻣ ي 33 / 0 - 17 /0 ﯽﻠﯿﻣ رد مﺮﮔﻮﻧﺎﻧ هدوﺪﺤﻣ و ﺮﺘﯿﻟ
شور ﯽﻄﺧ ي هﺮﺘﺴﮔ رد
ي 2000 - 63 / 0 رد مﺮﮔﻮﻧﺎﻧ
ﯽﻠﯿﻣ ﺪﻧﺪﻣآ ﺖﺳﺪﺑ ﺮﺘﯿﻟ .
ﺎﻨﺻ و مﻮﻠﻋ ﻪﻠﺠﻣ
ﯾ اﺬﻏ ﻊ ﯽﯾ ا ﯾ ناﺮ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣ ﺖﯾﺎﺳ
ﻤﻠﻋ ﻪﻟﺎﻘﻣ ﯽ ﺸﻫوﮋﭘ_ ﯽ
1 - ﻪﻣﺪﻘﻣ
ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﯽﺘﻧآ زا ﯽﮑﯾ ﺎﻫ
ﮏﯿﺗﻮﯿﺑ ﺞﯾار يﺎﻫ رد فﺮﺼﻣﺮﭘ و
ﯽﺴﮐا ﻞﻣﺎﺷ ﺎﺗﺪﻤﻋ ﻪﮐ ﺪﻨﺘﺴﻫ ماد شروﺮﭘ ﺖﻌﻨﺻ ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ
،1
ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﯽﺴﮐاد ،2
ﻦﯿﻠﮑﯾﺎﺳ ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗﺮﻠﮐ و 3
يور و هدﻮﺑ 4
هدﺮﺘﺴﮔ ﻒﯿﻃ يﺮﺘﮐﺎﺑ زا يا
ﺪﻧﺮﺛﻮﻣ ﺎﻫ ]
1 .[
زا ﻪﯾور ﯽﺑ هدﺎﻔﺘﺳا
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ ﺮﺠﻨﻣ ماد شروﺮﭘ ﺖﻌﻨﺻ رد ﺎﻫ ﻪﺑ
ﺶﯾاﺰﻓا
هﺪﻧﺎﻤﯿﻗﺎﺑ يﺎﻫ داﻮﻣ ﻦﯾا رد
تﻻﻮﺼﺤﻣ ﯽﯾاﺬﻏ
ﺪﻨﻧﺎﻣ ﯽﻣاد
ﺖﺷﻮﮔ
، هدرواﺮﻓ و ﺮﯿﺷ نآ يﺎﻫ
ﺎﻫ و ﺖﯾﺎﻬﻧ رد ﻦﯾا ﯽﺑﺎﯿﻫار
هﺪﻧﺎﻤﯿﻗﺎﺑ ﯽﻣ نﺎﺴﻧا نﺪﺑ ﻪﺑ ﺎﻫ دﻮﺷ
] 2 [.
ﯽﮔدﻮﻟآ ﯽﯾاﺬﻏ هدﺎﻣ
ﺪﻨﻧﺎﻣ ﯽﻓﺮﺼﻣﺮﭘ و ﺪﻨﻣدﻮﺳ ﻪﺑ ﺮﯿﺷ
زا هوﺮﮔ ﻦﯾا ﯽﺘﻧآ
ﮏﯿﺗﻮﯿﺑ ﺎﻫ
ﯾاﺰﻓا ﻞﻀﻌﻣ ﻪﺑ ندز ﻦﻣاد ﺮﺑ هوﻼﻋ ﺖﻣوﺎﻘﻣ ﺶ
ﯽﺘﻧآ يﺎﻫ ،ﯽﮑﯿﺗﻮﯿﺑ
ﺖﯿﻤﻫاﺮﭘ ﯽﻧﺪﻌﻣ هدﺎﻣ ﻦﯾا ناﺰﯿﻣ و هﺪﺷ ﺮﯿﺷ ﻢﯿﺴﻠﮐ رﺎﻬﻣ ﻪﺑ ﺮﺠﻨﻣ ﯽﻣ ار ﺪﻫﺎﮐ ] 3[
. ﯽﺘﻧآ زا ﻪﺘﺳد ﻦﯾا ﺶﯾﺎﭘ ﻦﯾاﺮﺑﺎﻨﺑ ﮏﯿﺗﻮﯿﺑ
رد ﺎﻫ
ﯽﻣ ﺖﯿﻤﻫا ﺰﺋﺎﺣ رﺎﯿﺴﺑ ﺮﯿﺷ ﺪﺷﺎﺑ
رد ﯽﻧاواﺮﻓ تﺎﻌﻟﺎﻄﻣ نﻮﻨﮐﺎﺗ و
درﻮﻣ ﻦﯾا هﺪﺷ مﺎﺠﻧا
ﺖﺳا ] 1 ، 3 ، 4 ، 5 .[
ﻪﺑ ﯽﺗاﺮﻃﺎﺨﻣ ﻞﯿﻟد ﻪﮐ
هﺪﻧﺎﻤﯿﻗﺎﺑ ﯽﺘﻧآ يﺎﻫ ياﺮﺑ ،دراد نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ ياﺮﺑ ﯽﮑﯿﺗﻮﯿﺑ
نآ راﺪﻘﻣ ﻦﯾا ﻪﮐ ﺖﺳا هﺪﺷ ﻒﯾﺮﻌﺗ زﺎﺠﻣ راﺪﻘﻣ ﻪﻨﯿﺸﯿﺑ ﺎﻫ ﻖﺒﻃ
ﺲﮐﺪﮐ ﻦﯿﻧاﻮﻗ ياﺮﺑ5
هﺪﻧﺎﻤﯿﻗﺎﺑ ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ ﯽﻨﯿﻠﮑﯾﺎﺳاﺮﺘﺗ يﺎﻫ رد
وﺎﮔ ﺮﯿﺷ 100 و ﺖﺳا هﺪﺷ ﻦﯿﯿﻌﺗ ﺮﺘﯿﻟ رد مﺮﮔوﺮﮑﯿﻣ دراﺪﻧﺎﺘﺳا
ناﺮﯾا ﯽﻠﻣ ﺰﯿﻧ
ﯽﺘﻧآ ﻪﺳ ياﺮﺑ ، ،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﮏﯿﺗﻮﯿﺑ
ﯽﺴﮐا وﺎﮔ ﺮﯿﺷ رد ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗﺮﻠﮐ و ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ 100
ﺮﺘﯿﻟ رد مﺮﮔوﺮﮑﯿﻣ ﺖﺳا هدﻮﻤﻧ ﻦﯿﯿﻌﻌﺗ ﻪﻨﯿﺸﯿﺑ زﺎﺠﻣ ﺪﺣ ناﻮﻨﻌﺑ ار
] 6 ، 7 [.
ﻢﻏﺮﯿﻠﻋ ﺖﻓﺮﺸﯿﭘ رد ﺮﯿﺧا ﻪﻫد ﺪﻨﭼ يﺎﻫ شور
ﻪﯾﺰﺠﺗ يﺎﻫ
،يا
هزاﺪﻧا نﺎﮑﻣا ﻢﯿﻘﺘﺴﻣ يﺮﯿﮔ
تﺎﺒﯿﮐﺮﺗ ﺖﻈﻠﻏ ﺎﺑ ﻢﮐ رﺎﯿﺴﺑ يﺎﻫ
ﻪﺑ ﻤﻧ رد هﮋﯾو ﻪﻧﻮ
ﻪﺑ هﺪﯿﭽﯿﭘ ﺖﻓﺎﺑ ياراد يﺎﻫ روﺪﻘﻣ ﻞﻣﺎﮐ رﻮﻃ
ﯽﻤﻧ ﺪﺷﺎﺑ . هدﺎﻣآ ﻞﺣاﺮﻣ زا هدﺎﻔﺘﺳا موﺰﻟ ﺮﻣا ﻦﯾا ﻪﻧﻮﻤﻧ يزﺎﺳ
ﺶﯿﭘ ﺖﻬﺟ ﻆﯿﻠﻐﺗ
ﺖﯿﻟﺎﻧآ ﺎﻫ ﺖﻤﺣاﺰﻣ فﺬﺣ و ﻪﻧﻮﻤﻧ ﺖﻓﺎﺑ يﺎﻫ
و
ﺖﯿﻟﺎﻧآ ﻞﯾﺪﺒﺗ ﻦﯿﻨﭽﻤﻫ ﻪﺑ ﺎﻫ
ﻢﺘﺴﯿﺳ و ﺰﯿﻟﺎﻧآ شور ﺎﺑ ﻖﺒﻄﻨﻣ مﺮﻓ
ﻪﯾﺰﺠﺗ ار يا نﺎﺸﻧ ﯽﻣ ﺪﻫد ] 8 [ . ﺴﻗ ﻦﯿﺑ ﻦﯾا رد نﺎﻣز هﺪﻤﻋ ﺖﻤ
ﻪﻧﻮﻤﻧ ﻪﯾﺰﺠﺗ ﯽﻣ ﺮﻈﻧ درﻮﻣ ﺐﯿﮐﺮﺗ جاﺮﺨﺘﺳا فﺮﺻ ﺎﻫ
ﻦﯾا و دﻮﺷ
ﻪﻤﻫ يور ﺮﺑ ﻪﻠﺣﺮﻣ ﻦﯾا زا ﺖﺳا راﺬﮔﺮﯿﺛﺄﺗ ﺰﯿﻟﺎﻧآ يﺪﻌﺑ ﻞﺣاﺮﻣ ي
1. Oxytetracyclin 2. Tetracycline 3. Doxycycline 4. Chlortetracycline 5. Codex
هزاﺪﻧا و ﯽﯾﺎﺳﺎﻨﺷ رد ﯽﺼﺧﺎﺷ ﺶﻘﻧ ور ﺖﯿﻟﺎﻧآ يﺮﯿﮔ
دراد ﺎﻫ ] 9 [.
شور ﻪﻤﻫ رد راﻮﺷد ﻪﻠﺣﺮﻣ ﺖﯿﻟﺎﻧآ ﺰﯿﻟﺎﻧآ يﺎﻫ
ﻢﻫ ﺮﯿﺷ رد ﺎﻫ
ﺖﯿﻟﺎﻧآ جاﺮﺨﺘﺳا ﺎﻫ
اﺮﭼ ﺖﺳا هﺪﯿﭽﯿﭘ ﯽﺴﮑﯾﺮﺗﺎﻣ ياراد ﺮﯿﺷ ﻪﮐ
ﯽﺑﺮﭼ زا ﻦﯿﺌﺗوﺮﭘ ،ﺎﻫ ﻻﻮﻤﻌﻣ و ﺖﺳا زﻮﺘﮐﻻ و ﯽﻧﺪﻌﻣ داﻮﻣ ،ﺎﻫ
شور يﺎﻫ ﻪﺑ ياﺮﺑ ﻪﺘﻓر رﺎﮐ ،ﺮﯿﺷ زا نﻮﮔﺎﻧﻮﮔ تﺎﺒﯿﮐﺮﺗ جاﺮﺨﺘﺳا
نﺎﻣز ﯽﻣ يدﺪﻌﺘﻣ ﻞﺣاﺮﻣ ياراد و هدﻮﺑ ﺮﺑ ﺪﻨﺷﺎﺑ
] 10 [ .
هدﺎﻣآ ياﺮﺑ هدﺎﻔﺘﺳا درﻮﻣ ﯽﻠﺻا شور ود جاﺮﺨﺘﺳا و يزﺎﺳ
ﻧآ ﺖﯿﻟﺎ ﻊﯾﺎﻣ جاﺮﺨﺘﺳا شور ﯽﯾاﺬﻏ داﻮﻣ زا ﻒﻠﺘﺨﻣ يﺎﻫ -
6ﻊﯾﺎﻣ ﺎﯾ
]LLE 11 ، 12 [ ﺪﻣﺎﺟ زﺎﻓ ﺎﺑ جاﺮﺨﺘﺳا شور و ﺎﯾ 7
SPE
] 13 ، 14 [ ﺖﺳا . ﺎﻫﺪﻌﺑ شور ﻪﯾﺎﭘ ﺮﺑ جاﺮﺨﺘﺳاﺰﯾر يﺎﻫ ﺶﻫﺎﮐ ي
جاﺮﺨﺘﺳا لﻼﺣ ﻢﺠﺣ هدﺎﺳ و ندﺮﮐ رﺎﮐدﻮﺧ ،هﺪﻨﻨﮐ
نﺪﺷ ﺮﺗ
ﻨﺘﻓﺮﮔراﺮﻗ ﻪﺟﻮﺗ درﻮﻣ و ﯽﻓﺮﻌﻣ جاﺮﺨﺘﺳا ﺪﻨﯾآﺮﻓ ﺪ.
جاﺮﺨﺘﺳاﺰﯾر
ﺪﻣﺎﺟ زﺎﻓ ﺎﺑ لﺎﺳ رد ﻪﮐ ﺖﺳا جاﺮﺨﺘﺳاﺰﯾر شور ﻦﯿﻟوا 8
1990
ﻪﺑ ﺶﯿﭘ ﺪﯾﺪﺟ شور ﮏﯾ ناﻮﻨﻋ هدﺎﻣآ و ﻆﯿﻠﻐﺗ
ﯽﻓﺮﻌﻣ ﻪﻧﻮﻤﻧ يزﺎﺳ
ﺪﺷ ] 15 [ . ﺶﯿﭘ و جاﺮﺨﺘﺳا نﺎﮑﻣا ﮏﯿﻨﮑﺗ ﻦﯾا زا هدﺎﻔﺘﺳا ﺎﺑ ﻆﯿﻠﻐﺗ
ﺖﯿﻟﺎﻧآ ﻪﺑ ﻪﻧﻮﻤﻧ ﺲﮑﯾﺮﺗﺎﻣ زا ﺎﻫ ﻢﻫ رﻮﻃ
ﻪﺑ دراد دﻮﺟو نﺎﻣز هوﻼﻋ
ﺎﻌﻣ ﮏﯿﻨﮑﺗ ﻦﯾا شور ﺐﯾ
ﯽﮕﺘﻓﺮﮔ ﺪﻨﻧﺎﻣ جاﺮﺨﺘﺳا ﺮﮕﯾد يﺎﻫ
رد ﺞﯾﺮﺗرﺎﮐ لﻼﺣ زا هدﺎﻔﺘﺳا و SPE
رد ﯽﻤﺳ يﺎﻫ ار LLE
دراﺪﻧ ] 16 [ .
فﺪﻫ ﺮﺿﺎﺣ ﯽﺸﻫوﮋﭘ رﺎﮐ ﻪﻌﺳﻮﺗ
ًﺎﺘﺒﺴﻧ و ﺪﻣآرﺎﮐ شور ﮏﯾ
ياﺮﺑ ﻊﯾﺮﺳ جاﺮﺨﺘﺳا
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ يﺎﻫ ،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ
ﯽﺴﮐا ﯽﺴﮐاد و ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗﺮﻠﮐ ،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﻦﯿﻠﮑﯾﺎﺳ
زا
ﻪﻧﻮﻤﻧ ﻫ ﯽﻣ ﺮﯿﺷ يﺎ ﺪﺷﺎﺑ
. ﻦﯾا ياﺮﺑ شور زا رﻮﻈﻨﻣ ﺰﯾر
جاﺮﺨﺘﺳا
ﯽﺸﺨﭘ ﺪﻣﺎﺟ زﺎﻓ هﺪﺷ هدﺎﻔﺘﺳا
هزاﺪﻧا و ﻪﺑ تﺎﺒﯿﮐﺮﺗ ﻦﯾا يﺮﯿﮔ
ﻪﯾارآ رﻮﺘﮑﺗد ﻪﺑ ﺰﻬﺠﻣ ﻻﺎﺑ ﯽﯾارﺎﮐ ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ شور دﻮﯾد
9ي ﺎﯾ HPLC-DAD ترﻮﺻ
ﺖﻓﺮﮔ . راﺪﻘﻣ ﻦﯿﻨﭽﻤﻫ
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ رد ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ 50
ﺑ وﺎﮔ مﺎﺧ ﺮﯿﺷ ﻪﻧﻮﻤﻧ سﺎﺳا ﺮ
هزاﺪﻧا و ﺶﯾﺎﭘ يدﺎﻬﻨﺸﯿﭘ شور ﺪﺷ يﺮﯿﮔ
.
2 - شور رﺎﮐ
2 - 1 - يرادﺮﺑ ﻪﻧﻮﻤﻧ ﯽﺋﺎﯿﻤﯿﺷ داﻮﻣ و
6 . Liquid liquid extraction 7. Solid phase extraction
8. Dispersive solid phase microextraction
9. High Performance Liquid Chromatography-diode array detector
ﯽﺸﻫوﮋﭘ رﺎﮐ ﻦﯾا رد 50
هﺰﯾرﻮﺘﺳﺎﭘﺮﯿﻏ و هﺰﯾرﻮﺘﺳﺎﭘ ﺮﯿﺷ ﻪﻧﻮﻤﻧ
لﺎﺳ نﺎﺘﺴﺑﺎﺗ و رﺎﻬﺑ رد 1399
ﻞﺤﻣ زا ﺰﯾﺮﺒﺗ ﺮﻬﺷ رد ﻪﺿﺮﻋ يﺎﻫ
ﻪﺑ و ﺬﺧا ﯽﻓدﺎﺼﺗ ترﻮﺻ ﺰﮐﺮﻣ ﻪﺑ تﺎﺸﯾﺎﻣزآ مﺎﺠﻧا ياﺮﺑ
ﯿﻘﺤﺗ ﻞﻘﺘﻨﻣ ﺰﯾﺮﺒﺗ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد يوراد و اﺬﻏ ﯽﻨﻤﯾا تﺎﻘ
و ﺪﻧﺪﺷ يراﺪﻬﮕﻧ لﺎﭽﺨﯾ يﺎﻣد رد .
ﻪﻣادا رد 50 ﯽﻠﯿﻣ ﺮﻫ زا ﺮﺘﯿﻟ
ﯽﻔﻨﻣ يﺎﻣد رد ﻪﻧﻮﻤﻧ 10
ﯽﺘﻧﺎﺳ ﻪﺟرد ﺖﻋﺮﺳ ﺎﺑ داﺮﮔ
4000 رود
تﺪﻣ ﻪﺑ ﻪﻘﯿﻗد رد 15
هﺪﺷ ﻊﻤﺟ ﻪﻣﺎﺧ و هﺪﺷ ژﻮﯿﻔﯾﺮﺘﻧﺎﺳ ﻪﻘﯿﻗد
ﺮﯿﺷ هﺪﻧﺎﻤﯿﻗﺎﺑ و اﺪﺟ ﻪﻧﻮﻤﻧ ﺮﻫ ﺢﻄﺳ رد درﻮﻣ ﺰﯿﻟﺎﻧآ ﺖﻬﺟ
ﺖﻓﺮﮔراﺮﻗ هدﺎﻔﺘﺳا .
زا ﮏﯾ ﭻﯿﻫ زا ﻪﮐ ﺮﯿﺷ ﻪﻧﻮﻤﻧ ﮏﯾ
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ هﺪﺸﻧ هدﺎﻔﺘﺳا نآ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
ﮏﯾ زا ،دﻮﺑ
زا نآ ندﻮﺑ يرﺎﻋ زا نﺎﻨﯿﻤﻇا زا ﺲﭘ و ﻪﯿﻬﺗ هﺪﻨﻨﮐ ﺪﯿﻟﻮﺗ هﺪﻧﺎﻤﯿﻗﺎﺑ ﯽﺘﻧآ يﺎﻫ اﺰﯾﻻا ﺖﯿﮐ زا هدﺎﻔﺘﺳا ﺎﺑ ﮏﯿﺗﻮﯿﺑ
) BT combo test kit (
ﻪﺑ ، ﺪﻨﯾاﺮﻓ رد ﮏﻧﻼﺑ ﻪﻧﻮﻤﻧ ناﻮﻨﻋ
ﻪﻨﯿﻬﺑ ﺖﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ شور يزﺎﺳ .
ﯽﺘﻧآ زا ردﺎﻣ لﻮﻠﺤﻣ ﮏﯿﺗﻮﯿﺑ
يﺎﻫ ،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ
ﯽﺴﮐا ﯽﺴﮐاد و ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗﺮﻠﮐ ،ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﻦﯿﻠﮑﯾﺎﺳ
) Sigma-Aldrich, USA (
ﺖﻈﻠﻏ ﻪﺑ 50
ﯽﻠﯿﻣ ﺮﺘﯿﻟ رد مﺮﮔ
ﺪﺷ ﻪﯿﻬﺗ لﻮﻧﺎﺘﻣ رد .
ﻦﯾا رد هدﺎﻔﺘﺳا درﻮﻣ ﯽﯾﺎﯿﻤﯿﺷداﻮﻣ ﺮﯾﺎﺳ
ﺖﮐﺮﺷ زا ﻖﯿﻘﺤﺗ Merck
ﻪﯾﺰﺠﺗ صﻮﻠﺧ ﻪﺟرد ﺎﺑ و نﺎﻤﻟآ يا
ﺖﺳا هدﻮﺑ .
2 - 2 -ﺑ ﻪﻨﯿﻬ جاﺮﺨﺘﺳا ﻂﯾاﺮﺷ يزﺎﺳ
ﺮﺛا ،ﻻﺎﺑ ﻆﯿﻠﻐﺗ رﻮﺘﮐﺎﻓ و جاﺮﺨﺘﺳا نﺎﻣﺪﻧار ﻪﺑ ﯽﺑﺎﯿﺘﺳد رﻮﻈﻨﻣ ﻪﺑ ﺮﺛﻮﻣ ﻞﻣاﻮﻋ ﺪﻨﻧﺎﻣ
راﺪﻘﻣ ﻮﺳر ﻞﻣﺎﻋ ب
هﺪﻨﻫد ) ﺮﯾدﺎﻘﻣ ﻦﯿﺑ زا 50
،
100 ، 150 ، 200 و 250 ﯽﻠﯿﻣ يﺮﺗ ﺪﯿﺳا زا مﺮﮔ ﮏﯿﺘﺳاوﺮﻠﮐ
( عﻮﻧ ،
بذﺎﺟ راﺪﻘﻣ و )
نﺎﯿﻣ زا ﺮﯾدﺎﻘﻣ 50 ، 100 ، 150 ، 200 و 250 زا
3 بذﺎﺟ C18
،
1 PSA و ﻦﺑﺮﮐ لﺎﻌﻓ هﺪﺷ ( ﯽﻧﻮﯾ ترﺪﻗ ، )
ﺎﺑ
ﺎﺗ ﺮﻔﺻ ﺮﯾدﺎﻘﻣ ندوﺰﻓا 10
ﯽﻧزو ﺪﺻرد /
ﮏﻤﻧ زا ﯽﻤﺠﺣ
(NaCl نﺎﻣز تﺪﻣ ، ﯽﺑد و
اﻮﻫ نﺪﯿﻣد )
ﯽﺳرﺮﺑ ﺎﺑ ﺖﻋﺮﺳ
نﺎﯾﺮﺟ يﺎﻫ 1/
0 ﺎﺗ 7/
0 ﯽﻠﯿﻣ ﻪﻘﯿﻗد رد ﺮﺘﯿﻟ هزﺎﺑ رد
5 ﯽﻟا 40
ﻪﯿﻧﺎﺛ ( ، ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﻢﺠﺣ و عﻮﻧ )
ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﻦﯿﺑ زا يﺎﻫ
ﺮﯾدﺎﻘﻣ رد ﻦﯿﺋﺎﺘﺑ ﻞﯿﺳدود و ﻦﯿﺋﺎﺘﺑ ،تﺎﺘﻧاز 500
ﯽﻟا 1000
ﯽﻠﯿﻣ ﻪﺑ مﺮﮔ ﻪﻧﺎﮔاﺪﺟ ترﻮﺻ (
، لﻼﺣ ﻢﺠﺣ و عﻮﻧ هﺪﻨﯾﻮﺷ
ﮏﯿﺘﮑﺗا ﻖﯿﻤﻋ ) ﺮﺑ ﺎﺑ ﯽﺳر ﻢﺠﺣ يﺎﻫ 100 ، 150 ، 200 ، 250 ،
300 و 400 لﻼﺣ زا ﮏﯾ ﺮﻫ زا ﺮﺘﯿﻟوﺮﮑﯿﻣ يﺎﻫ
1 Primary secondary amine .
يﺮﺗ ﻢﯿﻧﻮﻣآ ﻞﯿﺗﻮﺑ ﺪﯾﺮﻠﮐ
- يﺮﺗ ،ﺪﯿﺳا ﮏﯿﺋﻮﻧﺎﭘوﺮﭘ ﻞﯿﺗﻮﺑ
ﻢﯿﻧﻮﻣآ ﺪﯾﺮﻠﮐ - ﻦﯿﻠﯿﺗا يﺮﺗ و لﻮﮑﯿﻠﮔ ﻢﯿﻧﻮﻣآ ﻞﯿﺗﻮﺑ
ﺪﯾﺮﻠﮐ - ﮏﯿﺘﺳا
ﺪﯿﺳا ( ﺪﻨﺘﻓﺮﮔراﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ جاﺮﺨﺘﺳا ﯽﯾارﺎﮐ يور ﺮﺑ .
ياﺮﺑ
ﺑ ﺮﺘﻣارﺎﭘ ﯽﺳرﺮ شور زا يدﺎﻬﻨﺸﯿﭘ شور رد ﺮﺛﻮﻣ يﺎﻫ
"
ﮏﯾ
نﺎﻣز ﮏﯾ رد ﺮﺘﻣارﺎﭘ
"
ﺪﺷ هدﺎﻔﺘﺳا .
ﻪﺴﯾﺎﻘﻣ ﺎﺑ ﻞﻣاﻮﻋ ﻦﯾا ﺮﯿﺛﺎﺗ
ﮏﯿﭘ ﺮﯾز ﺢﻄﺳ ﺖﯿﻟﺎﻧآ زا ﻞﺻﺎﺣ
درﻮﻣ ﻒﻠﺘﺨﻣ ﻂﯾاﺮﺷ رد ﺎﻫ
ﻪﺘﻓﺮﮔ راﺮﻗ ﯽﺑﺎﯾزرا ﺪﻧا
] 5 [.
2 - 3 - جاﺮﺨﺘﺳا شور
روﺎﻨﺷ ﯽﺸﺨﭘ ﺪﻣﺎﺟ زﺎﻓ جاﺮﺨﺘﺳاﺰﯾر شور ﯽﺸﻫوﮋﭘ رﺎﮐ ﻦﯾا رد ﺷ ﺎﺑ و اﻮﻫ ﺎﺑ هﺪ لﻼﺣ دﺮﺑرﺎﮐ
ﻪﺑ ﻖﯿﻤﻋ ﮏﯿﺘﮑﺗا يﺎﻫ ناﻮﻨﻋ
ﺮﮕﺸﯾﻮﺷ ﺶﯿﭘ و جاﺮﺨﺘﺳا ياﺮﺑ
ﯽﺘﻧآ ﻆﯿﻠﻐﺗ ﮏﯿﺗﻮﺑ
يﺎﻫ
ﺮﯿﺷ ﻪﻧﻮﻤﻧ زا ﯽﻨﯿﻠﮑﯾﺎﺳاﺮﺘﺗ هداد ﻪﻌﺳﻮﺗ
ﺪﺷ ه ﺖﺳا . راﺪﻘﻣ اﺪﺘﺑا 10
ﯽﻠﯿﻣ ﺖﻈﻠﻏ ﺎﺑ هﺪﺷ هدﻮﻟآ ﺪﻫﺎﺷ ﺮﯿﺷ ﻪﻧﻮﻤﻧ زا ﺮﺘﯿﻟ 25
رد مﺮﮔﻮﻧﺎﻧ
ﯽﻠﯿﻣ ﯽﺘﻧآ زا ماﺪﮐ ﺮﻫ زا ﺮﺘﯿﻟ ﮏﯿﺗﻮﯿﺑ
ﺎﯾ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
ﻪﻧﻮﻤﻧ و ﻞﻘﺘﻨﻣ ﺶﯾﺎﻣزآ ﻪﻟﻮﻟ ﮏﯾ ﻞﺧاد ﻪﺑ ﯽﻘﯿﻘﺣ يﺎﻫ 150
ﯽﻠﯿﻣ يﺮﺗ ﺪﯿﺳا مﺮﮔ ﺪﺷ ﻪﻓﺎﺿا ﻪﻧﻮﻤﻧ ﻞﺧاد ﻪﺑ ﮏﯿﺘﺳاوﺮﻠﮐ
.
ﻪﺑ ﻞﺻﺎﺣ طﻮﻠﺨﻣ نآ زا ﺲﭘ و ﺲﮑﺗرو ﻪﻘﯿﻗد ﮏﯾ تﺪﻣ
ﺪﯾدﺮﮔ ژﻮﻔﯾﺮﺘﻧﺎﺳ .
ﻪﺑ و ﻪﺘﺷادﺮﺑ ﯽﯾور زﺎﻓ ﻞﮐ ﺪﻌﺑ ﻪﻠﺣﺮﻣ رد
ﺖﺳد جاﺮﺨﺘﺳا ﻪﻟﻮﻟ ﮏﯾ ﻞﺧاد زﺎﺳ
) ﮑﺷ ﻮﯾ ﻞ ( ﺪﺷ ﻞﻘﺘﻨﻣ . ﺲﭙﺳ
100 ﯽﻠﯿﻣ ﻪﺑ لﺎﻌﻓ ﻦﺑﺮﮐ بذﺎﺟ زا مﺮﮔ هاﺮﻤﻫ
500 ﯽﻠﯿﻣ مﺮﮔ
ﻪﺑ ﻦﯿﺋﺎﺘﺑ ﺪﯾدﺮﮔ ﻪﻓﺎﺿا لﻮﻠﺤﻣ ﻞﺧاد ﻪﺑ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ناﻮﻨﻋ .
ﺲﭘ
ﯽﺑد ﺎﺑ اﻮﻫ نﺎﯾﺮﺟ ﻪﺑ هﺪﯿﻤﺧ ﻪﻟﻮﻟ ﮏﯾرﺎﺑ ﺖﻤﺴﻗ نآ زا 4/
0
ﯽﻠﯿﻣ تﺪﻣ ﻪﺑ ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﯿﻟ 30
اﻮﻫ و ﻦﯿﺋﺎﺘﺑ ﺎﺗ ﺪﺷ ﻞﺼﺘﻣ ﻪﯿﻧﺎﺛ
بذﺎﺟ نﺪﺷ ﻊﻤﺟ ﺚﻋﺎﺑ دﻮﺷ لﻮﻠﺤﻣ ﺢﻄﺳ رد لﺎﻌﻓ لﺎﻏز
. رد
ﮏﯾ ﻞﺧاد ﻪﺑ و ﻪﺘﺷادﺮﺑ لﻮﺗﺎﭙﺳا ﮏﯾ ﺎﺑ ﯽﯾور زﺎﻓ ﻪﻠﺣﺮﻣ ﻦﯾﺮﺧآ نآ يور ﺮﺑ و هﺪﺷ ﻞﻘﺘﻨﻣ بﻮﯿﺗوﺮﮑﯿﻣ 250
لﻼﺣ زا ﺮﺘﯿﻟوﺮﮑﯿﻣ
يﺮﺗ ﻖﯿﻤﻋ ﮏﯿﺘﮑﺗا مﻮﯿﻧﻮﻣآ ﻞﯿﺗﻮﺑ
ﺪﯾﺮﻠﮐ - ﺪﯿﺳا ﮏﯿﺋﻮﻧﺎﭘوﺮﭘ
ﻪﺘﺨﯾر ﺪﺷ . ﺖﯿﻟﺎﻧآ ﯽﺑﺬﺟاو زا ﺲﭘ ﻪﺑ ﺎﻫ
،ﮏﯿﻧﻮﺳاﺮﺘﻟوا جاﻮﻣا ﮏﻤﮐ
ﻮﻠﺤﻣ هﺎﮕﺘﺳد ﻪﺑ ﯽﯾور ل HPLC
) Agilent1200- DAD
system, USA (
ﺪﺷ ﻖﯾرﺰﺗ .
2 - 4 - هﺎﮕﺘﺳد ﺎﺑ ﺰﯿﻟﺎﻧآ ﻂﯾاﺮﺷ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ
ﻪﺑ رد دﺎﻤﺘﻋا ﻞﺑﺎﻗ ﺞﯾﺎﺘﻧ و ﮏﯿﮑﻔﺗ ﺮﺜﮐاﺪﺣ ﻪﺑ ﯽﺑﺎﯿﺘﺳد رﻮﻈﻨﻣ
ﺖﯿﻟﺎﻧآ يزﺎﺳاﺪﺟ ،ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ ﻢﺘﺴﯿﺳ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ رد ﺎﻫ
ﺖﻓﺮﮔ ترﻮﺻ .
ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ
هﺎﮕﺘﺳد ﺰﯿﻟﺎﻧآ ياﺮﺑ ﯽﺘﻧآ
ﮏﯿﺗﻮﯿﺑ ﺎﻫ
لوﺪﺟ رد 1 هﺪﺷ ﺮﮐذ ﺖﺳا .
Table 1 Optimized condition of the HPLC-DAD for analysis of the selected tetracyclins in cow milk
Zorbax SB–Aq C18 column, l=10 cm, ID= 4.6 mm, Particle size= 3 μm Column
Gradient elution Elution type
A gradient elution composed of solvent A (water containing formic acid (0.5%), and solvent B (acetonitrile: methanol, 33.3:66.6 (v/v)) were used as
follows: from 0.0 to 3.0 min, 80% A and 20% B, from 3.0 to 6.0 min, a linear gradient from 20 to 80% B; from 6.0 to 7.0 min, 80% A and 20% B.
Mobile phase
Temperature: 40 °C, Loop= 50 μL Injector
Monitoring of the analytes was done at 335 nm for tetracycline and oxytetracycline, and 296 nm doxycycline and Chlortetracycline Detection wavelength
2 - 5 - ﯽﺳرﺮﺑ ﻪﯾﺰﺠﺗ تﺎﺼﺨﺸﻣ يا
ﻪﺑ ﻪﺘﻓررﺎﮑﺑ شور ﯽﺠﻨﺳرﺎﺒﺘﻋا رﻮﻈﻨﻣ مﻮﻤﺳ ﺰﯿﻟﺎﻧآ ياﺮﺑ
،يﺮﯿﮔرﺎﯿﻌﻣ رادﻮﻤﻧ ﻢﯿﺳﺮﺗ زا ﺲﭘ ،ﻖﯿﻘﺤﺗ ﻦﯾا رد هﺮﻔﺴﻓﻮﻧﺎﮔرا شور ﯽﻄﺧ هدوﺪﺤﻣ ) 1
(LR ، ﺺﯿﺨﺸﺗﺪﺣ ) 2
(LOD ،
هزاﺪﻧاﺪﺣ
3يﺮﯿﮔ ) (LOQ يﺮﯾﺬﭘراﺮﮑﺗ ، ) 4
(،RSD%
نﺎﻣﺪﻧار
جاﺮﺨﺘﺳا ) 5
(ER و ﻆﯿﻠﻐﺗ رﻮﺘﮐﺎﻓ ) 6
(EF و ﯽﺳرﺮﺑ ﻪﺒﺳﺎﺤﻣ
ﺪﻧﺪﺷ ] 17 [.
2 - 6 - ﻪﻧﻮﻤﻧ رد ﺲﮑﯾﺮﺗﺎﻣ ﺮﺛا ﯽﺳرﺮﺑ ﺎﻫ
ﺮﯿﺷ ي
ﻦﯿﺌﺗوﺮﭘ ﻪﻠﻤﺟ زا ﯽﻔﻠﺘﺨﻣ داﻮﻣ يﺎﻫﺪﻨﻗ ،ﺎﻫﺪﯿﭙﯿﻟ ،ﺎﻫ
ﻦﮑﻤﻣ ﺮﯿﺷ
هزاﺪﻧا رد ﺖﺳا ﯽﺘﻧآ يﺮﯿﮔ
ﮏﯿﺗﻮﯿﺑ دﺎﺠﯾا ﻞﺧاﺪﺗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
ﻪﺑ اﺬﻟ ﺪﯾﺎﻤﻧ ﻪﻧﻮﻤﻧ رد ﺲﮑﯾﺮﺗﺎﻣ ﺮﺛا ﯽﺳرﺮﺑ رﻮﻈﻨﻣ
،ﺮﯿﺷ يﺎﻫ 10
ﯽﻠﯿﻣ ﻪﻧﻮﻤﻧ زا ﺮﺘﯿﻟ ﺖﻈﻠﻏ ﺎﺑ ﯽﻘﯿﻘﺣ يﺎﻫ
يﺎﻫ 2 ، 5 و 10 مﺮﮔﻮﻧﺎﻧ
ﯽﻠﯿﻣ ﺮﺑ ﺴﻧ ﺮﺘﯿﻟ ﮏﯿﺗﻮﯿﺑ ﯽﺘﻧآ زا ماﺪﮐ ﺮﻫ ﻪﺑ ﺖﺒ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ
هدﻮﻟآ ﺎﺑ و جاﺮﺨﺘﺳا يدﺎﻬﻨﺸﯿﭘ شور ﺎﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﺖﺤﺗ و هﺪﺷ
ﺖﻓﺮﮔراﺮﻗ ﺰﯿﻟﺎﻧآ درﻮﻣ HPLC .
ﺲﭙﺳ 10 ﯽﻠﯿﻣ هﺰﯿﻧﻮﯾد بآ ﺮﺘﯿﻟ
ﺖﻈﻠﻏ نﺎﻤﻫ ﺎﺑ ﺖﯿﻟﺎﻧآ زا ﺎﻫ
جاﺮﺨﺘﺳا درﻮﻣ ﺲﭙﺳ و ﺪﺷ هدﻮﻟآ ﺎﻫ
ﺖﻓﺮﮔراﺮﻗ ﺰﯿﻟﺎﻧآ و .
لﺎﻨﮕﯿﺳ ﻪﺴﯾﺎﻘﻣ ﺎﺑ ﺰﺠﺗ يﺎﻫ
ﻪﯾ هﺪﻣآ ﺖﺳﺪﺑ يا
ﻪﻧﻮﻤﻧ زا ﮏﯾ ﺮﻫ ياﺮﺑ ﺲﮑﯾﺮﺗﺎﻣ ﺮﺛا ،هﺰﯿﻧﻮﯾد بآ و ﺮﯿﺷ يﺎﻫ
ﺖﻓﺮﮔراﺮﻗ ﯽﺑﺎﯾزرا درﻮﻣ ]
18 [.
3 - ﺚﺤﺑ و ﺞﯾﺎﺘﻧ
3 - 1 - ﻪﻨﯿﻬﺑ ﺞﯾﺎﺘﻧ رد ﺮﺛﻮﻣ ﻞﻣاﻮﻋ يزﺎﺳ
جاﺮﺨﺘﺳا
3 - 1 - 1 - بﻮﺳر هدﺎﻣ راﺪﻘﻣ هﺪﻨﻫد
1. Linear range 2. Limit of detection 3. Limit of quantitation 4. Relative standard deviation 5. Extraction recovery 6. Enrichment factor
بﻮﺳر ﻞﻣاﻮﻋ ﯽﻣ هﺪﻨﻫد
ﺪﻨﻧﺎﻣ ﯽﻠﻣاﻮﻋ ﺖﻤﺣاﺰﻣ ﺪﻨﻧاﻮﺗ
ﻦﯿﺌﺗوﺮﭘ ﯽﻠﭘ ،ﺎﻫ ﺎﮐﺎﺳ ﺎﻫﺪﯾر
، ﻪﻧاﺪﮕﻧر ﺪﻨﻨﮐ فﺬﺣ ار هﺮﯿﻏ و ﺎﻫ .
رد
يﺮﺗ ﺪﯿﺳا نﺎﯿﻣ ﻦﯾا ﺖﯾﺰﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﮏﯿﺘﺳاوﺮﻠﮐ
دراد ﻪﮐ ﯽﯾﺎﻫ
ﻪﺑ هدﺮﺘﺴﮔ رﻮﻃ ﯽﻣ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ يا
ﻪﮐ ﻦﯾا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ دﺮﯿﮔ
يﺮﺗ ﺪﯿﺳا ﻢﮐ راﺪﻘﻣ بﻮﺳر ﻪﺑ ردﺎﻗ ﮏﯿﺘﺳاوﺮﻠﮐ
مﺎﻤﺗ ﯽﻫد
ﻦﯿﺌﺗوﺮﭘ ﯽﻣ نآ دﺎﯾز راﺪﻘﻣ و ﺖﺴﯿﻧ ﺎﻫ ﺖﯿﻫﺎﻣ ﺪﻧاﻮﺗ
ار ﻪﻧﻮﻤﻧ
ﻪﻨﯿﻬﺑ ،ﺪﻫد ﺮﯿﯿﻐﺗ ﯽﻣ ﺖﯿﻤﻫا ﺰﺋﺎﺣ نآ راﺪﻘﻣ يزﺎﺳ
ﺪﺷﺎﺑ ] 19 .[
ﻞﮑﺷ رد هﺪﻣآ ﺖﺳد ﻪﺑ ﺞﯾﺎﺘﻧ 3
- 1 جاﺮﺨﺘﺳا ﯽﯾارﺎﮐ ﻪﮐ داد نﺎﺸﻧ
راﺪﻘﻣ ﺎﺗ 150 ﯽﻠﯿﻣ زا مﺮﮔ ﺪﯿﺳا يﺮﺗ ﮏﯿﺘﺳاوﺮﻠﮐ و ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا
ﯽﻣ ﯽﻗﺎﺑ ﺖﺑﺎﺛ ﺎﺒﯾﺮﻘﺗ نآ زا ﺲﭘ ﺪﻧﺎﻣ
. ﻦﯾاﺮﺑﺎﻨﺑ 150 ﯽﻠﯿﻣ ﻪﺑ مﺮﮔ
ﯿﻬﺑ راﺪﻘﻣ ناﻮﻨﻋ ﻪﻨ
ﺪﯿﺳا يﺮﺗ ﮏﯿﺘﺳاوﺮﻠﮐ بﺎﺨﺘﻧا
ﺷ ﺪ.
Fig1 Optimization of precipitating agent amount.
3 - 1 - 2 - عﻮﻧ هدﺎﻣ راﺪﻘﻣ و بذﺎﺟ
ﺪﻣﺎﺟ هدﺎﻣ ﮏﯾ زا ﯽﺸﻫوﮋﭘ رﺎﮐ ﻦﯾا رد جاﺮﺨﺘﺳا ياﺮﺑ
ﺖﯿﻟﺎﻧآ ﻪﻧﻮﻤﻧ زا ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ هدﺎﻔﺘﺳا ﺮﯿﺷ يﺎﻫ
ﺪﺷ . هدﺎﻣ
اد و دﺎﻌﺑا ياراد ﯽﺘﺴﯾﺎﺑ ﺪﻣﺎﺟ ﻪﻧ
يﺪﻨﺑ ﺪﻧاﻮﺘﺑ ﺎﺗ ﺪﺷﺎﺑ ﻢﻈﻨﻣ يﺎﻫ
ﻪﺑ ﺖﯿﻟﺎﻧآ يوﺎﺣ لﻮﻠﺤﻣ ﻞﺧاد رد ﺖﺧاﻮﻨﮑﯾ رﻮﻃ ددﺮﮔ ﺶﺨﭘ ﺎﻫ
.
ﻪﻧﻮﻤﻧ ﻞﺧاد زا نﺪﺷاﺪﺟ ﺖﯿﻠﺑﺎﻗ ﺪﯾﺎﺑ بذﺎﺟ ﻦﯾا ﺮﮕﯾد فﺮﻃ زا ﯽﯾﻻﺎﺑ بﺬﺟ ترﺪﻗ زا و ﻪﺘﺷاد ار ژﻮﯿﻔﯾﺮﺘﻧﺎﺳ ﺪﻨﯾاﺮﻓ ﯽﻃ ﺮﯿﺷ ﺪﺷﺎﺑ رادرﻮﺧﺮﺑ ]
20 .[
بذﺎﺟ ﻪﺳ ﻂﯾاﺮﺷ ﻦﯾا ﻦﺘﻓﺮﮔ ﺮﻈﻧ رد ﺎﺑ
C18
،
و PSA ﻦﺑﺮﮐ لﺎﻌﻓ ﻪﺑ هﺪﺷ جاﺮﺨﺘﺳا بذﺎﺟ ناﻮﻨﻋ هﺪﻨﻨﮐ
ﺪﯾدﺮﮔ بﺎﺨﺘﻧا .
زا هدﺎﻔﺘﺳا رد هﺪﻣآ ﺖﺳﺪﺑ ﺞﯾﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﯽﻣ راﺪﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ ﻪﺑ جاﺮﺨﺘﺳا ﯽﯾارﺎﮐ لﺎﻌﻓ ﻦﺑﺮﮐ ﺪﺳر
. راﺪﻘﻣ
ﺮﺘﻣارﺎﭘ وﺰﺟ ﺰﯿﻧ بذﺎﺟ جاﺮﺨﺘﺳاﺰﯾر شور ﯽﯾارﺎﮐ رد ﺮﺛﻮﻣ يﺎﻫ
ﯽﻣ ﯽﺸﺨﭘ ﺪﻣﺎﺟ زﺎﻓ ﺪﺷﺎﺑ
. ﯾارﺎﮐ رد ﺮﯿﺛﺎﺗ ﻦﯾا ﻪﺑ جاﺮﺨﺘﺳا ﯽ
ﺖﺳا طﻮﺑﺮﻣ ﻪﻧﻮﻤﻧ لﻮﻠﺤﻣ ﻢﺠﺣ ﻪﺑ بذﺎﺟ ﺪﻣﺎﺟ زﺎﻓ ﺖﺒﺴﻧ ﻪﺑ نﺎﻣﺪﻧار و هدﻮﺑ ﻢﮐ زﺎﻓ ﺖﺒﺴﻧ بذﺎﺟ ﻢﮐ ﺮﯾدﺎﻘﻣ رد ﻪﮑﯾرﻮﻃ
ﯽﻣ ﻦﯿﯾﺎﭘ جاﺮﺨﺘﺳا ﺎﻫزﺎﻓ ﺖﺒﺴﻧ بذﺎﺟ راﺪﻘﻣ ﺶﯾاﺰﻓا ﺎﺑ و ﺪﺷﺎﺑ
ﯽﻣ ﺶﯾاﺰﻓا جاﺮﺨﺘﺳا نﺎﻣﺪﻧار و ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا ﺪﺑﺎﯾ
] 20 .[
ﻦﯾا زا
ﻪﻨﯿﻬﺑ ﺪﯾﺎﺑ بذﺎﺟ راﺪﻘﻣ ور دﻮﺷ
. ﺶﯾﺎﻤﻧ ﺞﯾﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
هداد ﻞﮑﺷ رد هﺪﺷ 3
- 3 راﺪﻘﻣ ، 100 ﯽﻠﯿﻣ ،لﺎﻌﻓ ﻦﺑﺮﮐ بذﺎﺟ مﺮﮔ
ﻪﺑ ﺪﯾدﺮﮔ بﺎﺨﺘﻧا ﻪﻨﯿﻬﺑ راﺪﻘﻣ ناﻮﻨﻋ .
Fig 2 Selection of sorbent type.
Fig 3 Optimization of sorbent amount.
3 - 1 - 3 - اﻮﻫ نﺪﯿﻣد ﯽﺑد و نﺎﻣز تﺪﻣ
ﻪﺑ زﺎﺳروﺎﻨﺷ و ﺶﺨﭘ رﻮﻈﻨﻣ نﺪﯿﻣد زا ﺮﺿﺎﺣ شور رد بذﺎﺟ ي
ﺪﺷ هدﺎﻔﺘﺳا اﻮﻫ .
ﺎﺑ بذﺎﺟ سﺎﻤﺗ ﺢﻄﺳ ﺶﯾاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ اﻮﻫ
ﻢﻫ و هﺪﺷ ﯽﺸﯾﺎﻣزآ لﻮﻠﺤﻣ ﮏﻤﮐ ﺎﺑ بذﺎﺟ يزﺎﺳروﺎﻨﺷ ﻪﺑ نﺎﻣز
ﯽﻣ ﮏﻤﮐ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﺪﻨﮐ
] 21 .[
ﻦﯾا زا اﻮﻫ نﺪﯿﻣد ﺖﻋﺮﺳ ور
تﺪﻣ و ﯽﺸﯾﺎﻣزآ لﻮﻠﺤﻣ ﻞﺧاد ﻪﺑ درﻮﻣ ﯽﺘﺴﯾﺎﺑ نآ نﺎﻣز
ﻪﻨﯿﻬﺑ دﺮﯿﮔراﺮﻗ يزﺎﺳ .ﺑ
نﺎﯾﺮﺟ ﺖﻋﺮﺳ ﺶﯾاﺰﻓا ﺎﺑ ﺞﯾﺎﺘﻧ سﺎﺳا ﺮ
ﺎﺗ اﻮﻫ 4/
0 ﯽﻠﯿﻣ لﺎﻨﮕﯿﺳ ﻪﻘﯿﻗد رد ﺮﺘﯿﻟ ﻪﯾﺰﺠﺗ يﺎﻫ
ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا يا
ﺖﺑﺎﺛ نآ زا ﺲﭘ و ﺖﻓﺎﯾ ﺶﻫﺎﮐ ًادﺪﺠﻣ و هﺪﻧﺎﻣ
. لﺎﻨﮕﯿﺳ ﺶﻫﺎﮐ ﺎﻫ
نﺎﯾﺮﺟ رد ﯽﻣ ﺮﺘﻌﯾﺮﺳ ياﻮﻫ
ﻪﺑ ﺪﻧاﻮﺗ تﺪﻣ ﻞﯿﻟد سﺎﻤﺗ ﻢﮐ نﺎﻣز
ﺪﺷﺎﺑ ﺶﯾﺎﻣزآ لﻮﻠﺤﻣ ﺎﺑ بذﺎﺟ .
ﻪﺑ ﺞﯾﺎﺘﻧ هﺪﻣآ ﺖﺳد ا
ﯽﺳرﺮﺑ ز
تﺪﻣ اﻮﻫ نﺪﯿﻣد نﺎﻣز ﺎﺗ جاﺮﺨﺘﺳا نﺎﻣﺪﻧار ﻪﮐ داد نﺎﺸﻧ ﺰﯿﻧ
تﺪﻣ نﺎﻣز 30 ﯽﻣ ﺖﺑﺎﺛ ًﺎﺒﯾﺮﻘﺗ نآ زا ﺲﭘ و ﺶﯾاﺰﻓا ﻪﯿﻧﺎﺛ ﺪﻧﺎﻣ
. زا
ﻦﯾا ور 30 ﺪﺷ بﺎﺨﺘﻧا تﺎﺸﯾﺎﻣزآ ﻪﻣادا رد ﻪﯿﻧﺎﺛ .
3 - 1 - 4 - ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﻢﺠﺣ و عﻮﻧ ﺪﻣﺎﺟ زﺎﻓ نﺪﺷ روﺎﻨﺷ ﻪﺑﺮﺠﻨﻣ اﻮﻫ نﺎﯾﺮﺟ رﺎﻨﮐ رد ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ
بذﺎﺟ زﺎﯿﻧ نوﺪﺑ نآ ندﺮﮐاﺪﺟ نﺎﮑﻣا و هﺪﺷ لﻮﻠﺤﻣ ﺢﻄﺳ رد
ﯽﻣ ﻢﻫاﺮﻓ ار ژﻮﯿﻔﯾﺮﺘﻧﺎﺳ ﻪﺑ ﺪﻨﮐ
. رﻮﻀﺣ ﺮﮕﯾد فﺮﻃ زا
ﺖﯿﻟﺎﻧآ بﺬﺟ ترﺪﻗ ﺶﯾاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﺢﻄﺳ ﻪﺑ ﺎﻫ
ﯽﻣ ﺪﻣﺎﺟ بذﺎﺟ دﻮﺷ
] 21 .[
ﻦﯾا زا ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﻢﺠﺣ و عﻮﻧ ور
دﺮﯿﮔ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﯽﺘﺴﯾﺎﺑ .
ﻦﯿﺑ رد ﻪﮐ داد نﺎﺸﻧ ﺞﯾﺎﺘﻧ
ﻮﺳ ﺖﻧﺎﺘﮐﺎﻓر ﯽﻨﻌﻣ توﺎﻔﺗ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ و دراﺪﻧ دﻮﺟو يراد
ﻞﺣاﺮﻣ ياﺮﺑ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ ﻦﯾا ،ﻦﯿﺋﺎﺘﺑ ندﻮﺑ سﺮﺘﺳد رد ﻞﯿﻟد ﻪﺑ ﺪﺷ بﺎﺨﺘﻧا ﯽﺗآ .
ﺞﯾﺎﺘﻧ ﺰﯿﻧ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ راﺪﻘﻣ ﯽﺑﺎﯾزرا داد نﺎﺸﻧ
ﺶﻫﺎﮐ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ راﺪﻘﻣ ﺶﯾاﺰﻓا ﺎﺑ جاﺮﺨﺘﺳا نﺎﻣﺪﻧار ﻪﮐ ﯽﻣ ﺪﺑﺎﯾ . ﺮﺘﻤﮐ ﺮﯾدﺎﻘﻣ رد ﻪﮑﯿﺋﺎﺠﻧآ زا زا
500 ﯽﻠﯿﻣ ﺪﻣﺎﺟ زﺎﻓ مﺮﮔ
تﺎﺸﯾﺎﻣزآ ﻪﻣادا اﺬﻟ ﺪﺸﻧ روﺎﻨﺷ ﯽﺸﯾﺎﻣزآ لﻮﻠﺤﻣ يور ﺮﺑ بذﺎﺟ ﺎﺑ 500 ﯽﻠﯿﻣ مﺎﺠﻧا ﻦﯿﺋﺎﺘﺑ زا مﺮﮔ ﺪﺷ
.
Fig4 Selection of surfactant type.
Fig 5 Optimization of surfactant amount.
هﺪﻨﯾﻮﺷ لﻼﺣ ﻢﺠﺣ و عﻮﻧ -5-1-3 لﻼﺣ زا ﺮﺿﺎﺣ ﺶﻫوﮋﭘ رد ﮑﺗا يﺎﻫ
ﻪﺑ ﻖﯿﻤﻋ ﮏﯿﺘ ﻞﯿﻟد
ﺖﯿﻤﺳ
ﻢﮐ ﺎﻬﻧآ ﺮﺗ هدﺎﻔﺘﺳا ﺪﺷ ] 14 .[
ﺮﺘﻣارﺎﭘ ﻦﯾا ﯽﺳرﺮﺑ زا ﻞﺻﺎﺣ ﺞﯾﺎﺘﻧ
لﻼﺣ ﻦﯿﺑ رد ﻪﮐ داد نﺎﺸﻧ يﺎﻫ
ﻖﯿﻤﻋ ﮏﯿﺘﮑﺗا هﺪﺷ بﺎﺨﺘﻧا
ﻪﮐ
ﺖﯿﻟﺎﻧآ ﺶﯾﻮﺷ ﺖﯿﻠﺑﺎﻗ ﯽﮔﮋﯾو ود ياراد ﺪﯾﺎﺑ هﺪﺷ بﺬﺟ يﺎﻫ
ﻂﺳﻮﺗ ﻞﺑﺎﻗ ﺎﺗ هدﻮﺑ ﯽﺑآ و ﯽﻟآ زﺎﻓ رد لﻼﺤﻧا ﺖﯿﻠﺑﺎﻗ و بذﺎﺟ
ﺘﺴﯿﺳ ﻪﺑ ﻖﯾرﺰﺗ ﻢ
ﺪﺷﺎﺑ HPLC ] 20 [ يﺮﺗ ، ﻢﯿﻧﻮﻣآ ﻞﯿﺗﻮﺑ
ﺪﯾﺮﻠﮐ - ار ﯽﯾارﺎﮐ ﻦﯾﺮﺘﺸﯿﺑ ﺪﯿﺳا ﮏﯿﺋﻮﻧﺎﭘوﺮﭘ ﺖﺷاد
. لﻼﺣ ﻢﺠﺣ
ﯽﻣ يدﺎﻬﻨﺸﯿﭘ شور رد ﺮﺛﻮﻣ ﺮﺘﻣارﺎﭘ ﺰﯿﻧ هﺪﻨﯾﻮﺷ ﺎﺑ ﻪﮐاﺮﭼ ﺪﺷﺎﺑ
ﻢﺠﺣ ﺶﯾاﺰﻓا
، ﺖﯿﻟﺎﻧآ ﻪﺘﺴﺷ زﺎﻓ رد ﺎﻫ و هدﻮﺑ ﻖﯿﻗر هﺪﺷ
ﯽﻣ ﻢﮐ ﻆﯿﻠﻐﺗرﻮﺘﮐﺎﻓ دﻮﺷ
] 22 [ رﻮﻣ ﯽﺘﺴﯾﺎﺑ لﻼﺣ ﻢﺠﺣ اﺬﻟ د
دﺮﯿﮔ راﺮﻗ يزﺎﺳ ﻪﻨﯿﻬﺑ .
ﻪﺘﻓﺎﯾ سﺎﺳا ﺮﺑ ﻢﺠﺣ ﺎﻫ
150 زا ﺮﺘﯿﻟوﺮﮑﯿﻣ
داد نﺎﺸﻧ جاﺮﺨﺘﺳا ﺪﻧور رد ار ﯽﯾارﺎﮐ ﻦﯾﺮﺗﻻﺎﺑ لﻼﺣ .
Fig 6 Selection of elution solvent type.
Fig7 Optimization of elution solvent volume.
3 - 1 - 6 - ﯽﻧز ﮏﻤﻧ ﺮﺛا شور رد ًﺎﻣﻮﻤﻋ
ﺶﯾاﺰﻓا ،جاﺮﺨﺘﺳا يﺎﻫ لﻮﻠﺤﻣ ﻪﺑ ﮏﻤﻧ
ﯽﺑآ يﺎﻫ
دراد ﻞﺑﺎﻘﺘﻣ ﺮﺛا ود .
يوﺮﯿﻧ ﺶﯾاﺰﻓا ﺚﻋﺎﺑ ﮏﻤﻧ رﻮﻀﺣ ﻪﮑﻨﯾا لوا
ﺖﯿﻟﺎﻧآ ﺖﯿﻟﻼﺣ ﺶﻫﺎﮐ ﺚﻋﺎﺑ و هﺪﺷ ﯽﺑآ زﺎﻓ ﯽﻧﻮﯾ ﯽﺑآ زﺎﻓ رد ﺎﻫ
نآ لﺎﻘﺘﻧا و جاﺮﺨﺘﺳا لﻼﺣ ﻞﺧاد ﻪﺑ ﺎﻫ
ﯽﻣ هﺪﻨﻨﮐ ﻪﺠﯿﺘﻧ رد دﻮﺷ
نﺎﻣﺪﻧار ﯽﻣ ﺶﯾاﺰﻓا جاﺮﺨﺘﺳا ﺪﺑﺎﯾ
. ﻪﺑ ﮏﻤﻧ ﺶﯾاﺰﻓا ﻪﮐ ﻦﯾا مود
ﺤﻣ ﯽﻣ جاﺮﺨﺘﺳا ﻂﯿ جاﺮﺨﺘﺳا لﻼﺣ ﺖﯿﻟﻮﻠﺤﻣ ﺪﻧاﻮﺗ
زﺎﻓ رد هﺪﻨﻨﮐ
ﯽﻟآ زﺎﻓ ﻢﺠﺣ ﺶﯾاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ و هداد ﺶﻫﺎﮐ ﺰﯿﻧ ار ﯽﺑآ ﻊﻤﺟ هﺪﺷ ﯽﻣ ﺮﻣا ﻦﯾا ﻪﮐ دﻮﺷ لﺎﻨﮕﯿﺳ ﺶﻫﺎﮐ ﺚﻋﺎﺑ ﺪﻧاﻮﺗ
ﻪﯾﺰﺠﺗ ﻖﯿﻗر ﺮﺛا رد يا دﻮﺷ يزﺎﺳ
] 23 .[
ﺮﻫ ﺮﺑ تاﺮﺛا زا ماﺪﮐ
ﻦﯾا رد ﺪﺷﺎﺑ ﻪﺘﺷاد يﺮﺗﺮﺑ يﺮﮕﯾد ﻋ نآ ﺮﯿﺛﺄﺗ ترﻮﺻ
ﺐﻟﺎﻏ ﻞﻣﺎ
دﻮﺑ ﺪﻫاﻮﺧ .
ﻪﻌﻟﺎﻄﻣ ﻦﯾا رد راﺪﻘﻣ ﺶﯾاﺰﻓا ﺎﺑ
نﺎﻣﺪﻧار NaCl
ﺶﻫﺎﮐ جاﺮﺨﺘﺳا ﺲﭘ ﺖﻓﺎﯾ
ﮏﻤﻧ بﺎﯿﻏ رد تﺎﺸﯾﺎﻣزآ
ترﻮﺻ ﺖﻓﺮﮔ .
3 - 2 - ﻪﯾﺰﺠﺗ تﺎﺼﺨﺸﻣ ﺞﯾﺎﺘﻧ شور يا
ﻪﯾﺰﺠﺗ يﺎﻫﺮﺘﻣارﺎﭘ ﺖﺳﺪﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﺖﺤﺗ ﺮﺿﺎﺣ شور يا
ﺪﻧﺪﻣآ . هداد ياﺮﺑ ﯽﻧﻮﯿﺳاﺮﮔر ﻪﻟدﺎﻌﻣ هدوﺪﺤﻣ رد ﻪﻠﺻﺎﺣ يﺎﻫ
درﻮﻣ ﯽﺳرﺮﺑ ) لﻮﻠﺤﻣ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﺑ يدراﺪﻧﺎﺘﺳا يﺎﻫ
ﺖﻈﻠﻏ يﺎﻫ 6/
0 ، 25 /1 ، 5/
2 ، 5 ، 10 ، 15 ، 30 ، 62 ، 125 ، 250 ،
500 و 2000 ﻮﻧﺎﻧ ﯽﻠﯿﻣ ﺮﺑ مﺮﮔ ﯽﺘﻧآ زا ﺮﺘﯿﻟ
ﮏﯿﺗﻮﯿﺑ درﻮﻣ يﺎﻫ
ﻪﻌﻟﺎﻄﻣ ( شور ﯽﻄﺧ هدوﺪﺤﻣ نﻮﯿﺳاﺮﺒﯿﻟﺎﮐ ﯽﻨﺤﻨﻣ زا و ﻢﯿﺳﺮﺗ
ﺖﯿﻟﺎﻧآ زا ﮏﯾ ﺮﻫ ياﺮﺑ ﺪﻣآ ﺖﺳﺪﺑ ﺎﻫ
. ﺪﺣ و ﺺﯿﺨﺸﺗ ﺪﺣ
هزاﺪﻧا ﺖﻈﻠﻏ ﺮﺑاﺮﺑ ﺐﯿﺗﺮﺗ ﻪﺑ شور يﺮﯿﮔ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﯽﯾﺎﻫ
ﺎﺑ ﺮﺑاﺮﺑ ﺐﯿﺗﺮﺗ ﻪﺑ ﺰﯾﻮﻧ ﻪﺑ لﺎﻨﮕﯿﺳ ﺖﺒﺴﻧ ﺎﻫ نآ رد ﻪﮐ ﺪﻧﺪﺷ 3
و
10 ﯽﻣ ﺪﻨﺷﺎﺑ . ﻪﺑ زا شور ﺖﻗد و يﺮﯾﺬﭘراﺮﮑﺗ ﯽﺳرﺮﺑ رﻮﻈﻨﻣ
ﯽﺒﺴﻧ دراﺪﻧﺎﺘﺳا فاﺮﺤﻧا )
(% RSD ﺪﺷ هدﺎﻔﺘﺳا .
ﻪﺒﺳﺎﺤﻣ
ﺖﯿﻟﺎﻧآ ﮏﯿﭘ ﺖﺣﺎﺴﻣ ﻪﺴﯾﺎﻘﻣ زا ﻆﯿﻠﻐﺗرﻮﺘﮐﺎﻓ لﻮﻠﺤﻣ يﺎﻫ
ﺖﯿﻟﺎﻧآ ﮏﯿﭘ ﺖﺣﺎﺴﻣ ﺎﺑ دراﺪﻧﺎﺘﺳا ﺲﭘ ﺮﯿﺷ رد دﻮﺟﻮﻣ يﺎﻫ
ترﻮﺻ جاﺮﺨﺘﺳا مﺎﺠﻧا زا ﺲﭘ و يدﺎﻬﻨﺸﯿﭘ شور ياﺮﺟا ﺖﻓﺮﮔ . ﻪﯾﺰﺠﺗ تﺎﺼﺨﺸﻣ زا ﻞﺻﺎﺣ ﺞﯾﺎﺘﻧ لوﺪﺟ رد شور يا
2
ﺖﺳا هﺪﺷ ﻪﺋارا .
Table 2 Quantitative features of the developed method for the selected tetracyclins
Analyte LRa LODb LOQc r2d RSD%e ER ± SDf EF ± SDg
Tetracycline 1.13-2000 0.33 1.13 0.995 4.9 84 ± 3 56 ± 3
Chlortetracycline 0.63-2000 0.17 0.63 0.994 5.9 91 ± 4 60± 8
Doxycycline 0.78-2000 0.23 0.78 0.998 8.3 90 ± 2 59 ± 4
Oxytetracycline 0.96-2000 0.29 0.96 0.999 6.6 80 ± 3 52± 3
a) Linear range (ng ml−1)
b) Limit of detection (S/N = 3) (ng ml−1) c) Limit of quantification (S/N = 10) (ng ml−1) d) Coefficient of determination
e) Relative standard deviation (n = 5)
f) Extraction recovery ± standard deviation (n = 3) g) Enrichment factor ± standard deviation (n = 3)
ﻪﺑ جاﺮﺨﺘﺳا رد يدﺎﻬﻨﺸﯿﭘ شور ﯽﯾارﺎﮐ ﯽﺑﺎﯾزرا رﻮﻈﻨﻣ
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ ﺮﻈﻧ درﻮﻣ يﺎﻫ ﻪﯾﺰﺠﺗ يﺎﻫﺮﺘﻣارﺎﭘ ﯽﺧﺮﺑ ،
شور يا
ﺪﺣ هدوﺪﺤﻣ ﻞﯿﺒﻗ زا ﺮﺿﺎﺣ ،ﺺﯿﺨﺸﺗ
هزاﺪﻧا ﺪﺣ ،يﺮﯿﮔ ﺪﺻرد
فاﺮﺤﻧا و دراﺪﻧﺎﺘﺳا ﺖﯿﻟﺎﻧآ ﺖﻓﺎﯾزﺎﺑ ناﺰﯿﻣ
،ﺎﻫ ﻪﺑ شور ﺪﻨﭼ ﺎﺑ رﺎﮐ
جاﺮﺨﺘﺳا ياﺮﺑ ﻪﺘﻓر ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ
ﺎﻫ ﻪﻧﻮﻤﻧ زا ﻪﺴﯾﺎﻘﻣ ﻒﻠﺘﺨﻣ يﺎﻫ
لوﺪﺟ رد ﻪﮐ ﺪﺷ 3
هﺪﺷ هرﺎﺷا نآ ﻪﺑ ﺖﺳا
. ﯽﮕﺘﺴﯾﺎﺷ مﺎﻗرا ﻪﻤﻫ
شور ﺎﺑ ﻪﺴﯾﺎﻘﻣ ﻞﺑﺎﻗ ﺮﺿﺎﺣ شور يﺎﻫ
ﺮﮕﯾد ﻪﺑ ﺖﺳا ﮑﯾرﻮﻃ ﻪ ﺪﺣ ﺮﯾدﺎﻘﻣ
هزاﺪﻧا ﻪﺑ هﺪﻣآ ﺖﺳﺪﺑ ﺺﯿﺨﺸﺗ ﺪﺣ و يﺮﯿﮔ ﻞﺑﺎﻗ رﻮﻃ
ﻦﯿﯾﺎﭘ ﻪﺟﻮﺗ شور ﺮﯾﺎﺳ زا ﺮﺗ
و هدﻮﺑ ﺎﻫ جاﺮﺨﺘﺳا نﺎﻣﺪﻧار
ﺖﯿﻟﺎﻧآ رد ﺎﻫ ﺮﺿﺎﺣ شور
، ﻦﯿﯾﺎﭘ ﯽﺘﺣ ﺎﯾ ﻪﺴﯾﺎﻘﻣ ﻞﺑﺎﻗ ﺮﯾﺎﺳ زا ﺮﺗ
شور ﺎﻫ ﯽﻣ ﺪﺷﺎﺑ . ندﻮﺑ اراد دراﺪﻧﺎﺘﺳا فاﺮﺤﻧا ﻦﯿﯾﺎﭘ راﺪﻘﻣ
نﺎﺸﻧ ﻪﮐ ﯽﺒﺴﻧ ﺎﺑ يﺮﯾﺬﭘراﺮﮑﺗ هﺪﻨﻫد
ﺖﺳا يدﺎﻬﻨﺸﯿﭘ شور يﻻ ،
ﺖﯾﺰﻣ ﺮﮕﯾد ﯽﻣ شور ﻦﯾا ﺪﺷﺎﺑ
.
Table 3: Comparison of the proposed method with other methods in the extraction and determination of the tetracyclins determined by HPLC.
Reference ERd
(%) RSDc
LOQb (%) LODa
Sample Sample preparation
method
24 75-102.6
˂15 ˂10 (ng g-1)
˂10 (ng g-1) Meat and liver
A
1 70-113
5.1-28.4 ˂8.4 (ng ml-1) 1.5-8.5
(ng ml-1) Milk
B
25 83.07-106.3
˂15.5 100.3-105.6
(ng ml-1) 100.6-109.7
(ng ml-1) Milk
C
26 76.5-95.5
10-33 ˂11 (ng g-1) 4.4-12
(ng g-1) Meat
D
Present work 80-91
0.63-1.13 ˂8.3 (ng ml-1) 0.17-0.33
(ng ml-1) Milk
E
A) Accelerated solvent extraction method. B) Dispersive liquid–liquid microextraction based on hydrophobic deep eutectic solvents. C) QuEChERS Dispersive Extraction method. D) solid liquid extraction performed by two phase freezing method. E) dispersive solid-phase micro-extraction method. a) Limit of detection. b) Limit of
quantification. c) Relative standard deviation. d) Extraction recovery.
3 - 3 - ﻪﻧﻮﻤﻧ ﺰﯿﻟﺎﻧآ ﺞﯾﺎﺘﻧ ﯽﻘﯿﻘﺣ يﺎﻫ
50 ﻪﻧﻮﻤﻧ و ﻆﯿﻠﻐﺗ يدﺎﻬﻨﺸﯿﭘ شور ﺎﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻖﺒﻃ ﺮﯿﺷ
ﺎﺑ ﺲﭙﺳ و ﺪﻧﺪﺷ ﺺﯿﻠﺨﺗ HPLC-DAD
راﺮﻗ ﺰﯿﻟﺎﻧآ درﻮﻣ
ﺪﻨﺘﻓﺮﮔ . ﻞﮑﺷ 3 ماﺮﮔﻮﺗﺎﻣوﺮﮐ ﻢﯿﻘﺘﺴﻣ ﻖﯾرﺰﺗ ﻪﺑ طﻮﺑﺮﻣ يﺎﻫ
ﯽﺘﻧآ دراﺪﻧﺎﺘﺳا ﮏﯿﺗﻮﯿﺑ
ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ )
ﺖﻈﻠﻏ ﻪﺑ ماﺪﮐ ﺮﻫ 10
ﯽﻠﯿﻣ ﺮﺘﯿﻟ رد مﺮﮔ (
ﻪﻧﻮﻤﻧ ﮏﯾ و نﺎﺸﻧ ار هﺪﺸﻧ ﮏﯾﺎﭙﺳا ﺮﯿﺷ ي
ﯽﻣ ﺪﻫد . ماﺮﮔﻮﺗﺎﻣوﺮﮐ ﻪﺴﯾﺎﻘﻣ ﻪﻧﻮﻤﻧ زا ﻞﺻﺎﺣ يﺎﻫ
ﺎﺑ ﺎﻫ
ﻪﻧﻮﻤﻧ زا ﻞﺻﺎﺣ ماﺮﮔﻮﺗﺎﻣوﺮﮐ ﻪﻤﻫ ﻪﮐ داد نﺎﺸﻧ دراﺪﻧﺎﺘﺳا
ﻪﻧﻮﻤﻧ ﻦﯿﯿﻌﺗ زﺎﺠﻣ ﺪﺣ زا ﺮﺗﻻﺎﺑ رد ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﻪﺑ هدﻮﻟآ ﺮﯿﺷ يﺎﻫ
ﯽﺘﻧآ ﺮﯾﺎﺳ و هدﻮﺑ ناﺮﯾا ﯽﻠﻣ دراﺪﻧﺎﺘﺳا ﻂﺳﻮﺗ هﺪﺷ ﮏﯿﺗﻮﯿﺑ
رد ﺎﻫ
ﻪﻧﻮﻤﻧ ﺪﻨﺘﺷاﺪﻧ دﻮﺟو ﺮﯿﺷ يﺎﻫ .
ﻪﺑ هزاﺪﻧا رﻮﻈﻨﻣ ﺖﻈﻠﻏ يﺮﯿﮔ
ﺖﯿﻟﺎﻧآ ﺪﺷ هدﺎﻔﺘﺳا دراﺪﻧﺎﺘﺳا ﺶﯾاﺰﻓا شور زا ﺎﻫ .
Fig 8 Typical HPLC–DAD chromatograms of direct injection of standard solution of the target
analytes at a concentration of 10 ng L−1 (I) and unspiked milk sample (II) after performing the
developed method. Peak identification: (1) Tetracycline, (2) Chlortetracycline, (3)
Doxycycline, (4) Oxytetracycline.
3 - 4 ﻪﻧﻮﻤﻧ رد ﺲﮑﯾﺮﺗﺎﻣ ﺮﺛا ﯽﺳرﺮﺑ - ﯽﻘﯿﻘﺣ يﺎﻫ
لﺎﻨﮕﯿﺳ ﻪﺴﯾﺎﻘﻣ ﺎﺑ ﺮﯿﺷ ﺲﮑﯾﺮﺗﺎﻣ ﺮﺛا ﻪﯾﺰﺠﺗ يﺎﻫ
هﺪﻣآ ﺖﺳﺪﺑ يا
ﻪﻧﻮﻤﻧ زا ﮏﯾ ﺮﻫ ياﺮﺑ بآ و ﯽﻘﯿﻘﺣ يﺎﻫ
ﯽﺑﺎﯾزرا درﻮﻣ هﺰﯿﻧﻮﯾد ﺖﻓﺮﮔراﺮﻗ
. ﺖﺳﺪﺑ ﺞﯾﺎﺘﻧ ﻪﻧﻮﻤﻧ ﺲﮑﯾﺮﺗﺎﻣ ﻪﮐ داد نﺎﺸﻧ هﺪﻣآ
ﺎﻫ
ﺪﻧراﺪﻧ يدﺎﻬﻨﺸﯿﭘ شور ﯽﯾارﺎﮐ رد ﯽﺳﻮﺴﺤﻣ ﺮﯿﺛﺄﺗ .
Table 4 Results of assays to check the sample matrix effect for the analytes Mean relative recovery ± standard deviation (n = 3)
Sample4 Sample3
Sample2 Sample1
Analyte
All samples were spiked with each analyte at a concentration of 2 ng ml−1 98 ± 4 90 ± 4
96 ± 4
97 ± 4
Tetracycline
97 ± 4 92 ± 4
94 ± 3
91 ± 5
Chlortetracycline
98 ± 5 99 ± 3
96 ± 4
92 ± 3
Doxycycline
98 ± 6 90 ± 5
93 ± 5
97 ± 4
Oxytetracycline
All samples were spiked with each analyte at a concentration of 5 ng ml−1 95 ± 4 97 ± 4
98 ± 4
94 ± 5
Tetracycline
92 ± 4 95 ± 5
93 ± 3
95 ± 7
Chlortetracycline
93 ± 7 96 ± 4
95 ± 5
94 ± 6
Doxycycline
98 ± 3 90 ± 6
96 ± 3
97 ± 3
Oxytetracycline
All samples were spiked with each analyte at a concentration of 10 ng ml−1 94 ± 3 97 ± 3
96 ± 2
97 ± 3
Tetracycline
98 ± 4 90 ± 4
91 ± 4
97 ± 5
Chlortetracycline
96 ± 5 92 ± 4
93 ± 4
99 ± 4
Doxycycline
95 ± 3 90 ± 4
94 ± 5
92 ± 4
Oxytetracycline
3 - 5 ﻪﺠﯿﺘﻧ - يﺮﯿﮔ ﯽﻠﮐ
ياﺮﺟا رد هداد ﻪﻌﺳﻮﺗ شور
زا ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد هﺪﺷ
ﻪﺑ يزﺎﺳروﺎﻨﺷ زﺎﻓ تارذ ﺶﺨﭘ ياﺮﺑ ﺖﻧﺎﺘﮐﺎﻓرﻮﺳ و اﻮﻫ ﮏﻤﮐ
لﻼﺣ زا و ﺪﻣﺎﺟ ﻪﺑ ﻖﯿﻤﻋ ﮏﯿﺘﮑﺗا يﺎﻫ
ﺖﯿﻟﺎﻧآ ﻦﺘﺴﺷ رﻮﻈﻨﻣ يﺎﻫ
بﺬﺟ ﺪﻣﺎﺟ زﺎﻓ رد هﺪﺷ ﺪﺷ هدﺎﻔﺘﺳا
. شور يﺎﯾاﺰﻣ ﻪﻠﻤﺟ زا
ﻨﺸﯿﭘ ﯽﻣ يدﺎﻬ ﻪﺑ ناﻮﺗ ،ندﻮﺑ نازرا ،ندﻮﺑ دﺎﻤﺘﻋا ﻞﺑﺎﻗ ،ﯽﮔدﺎﺳ
لﻼﺣ ﻢﮐ فﺮﺼﻣ دﺮﮐ هرﺎﺷا هﺎﺗﻮﮐ ﺰﯿﻟﺎﻧآ نﺎﻣز و ﯽﻟآ يﺎﻫ
. ﺞﯾﺎﺘﻧ
ﻪﺑ ﺮﺘﻣارﺎﭘ هﺪﻨﻫد نﺎﺸﻧ هﺪﻣآ ﺖﺳد ﻪﯾﺰﺠﺗ يﺎﻫ
ﯽﻣ بﻮﻠﻄﻣ يا ﺪﻨﺷﺎﺑ
ﻪﺑ نﺎﻣﺪﻧار ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﺖﺤﺗ ﻪﮑﯾرﻮﻃ يﺎﻫ
جاﺮﺨﺘﺳا 91
- 80
هدوﺪﺤﻣ رد ﺺﯿﺨﺸﺗ دوﺪﺣ و ﺪﺻرد ي
33 /0 - 17 /0 رد مﺮﮔﻮﻧﺎﻧ
ﯽﻠﯿﻣ هدوﺪﺤﻣ و ﺮﺘﯿﻟ هﺮﺘﺴﮔ رد شور ﯽﻄﺧ ي
ي 2000 - 63 /0
ﯽﻠﯿﻣ رد مﺮﮔﻮﻧﺎﻧ ﺪﻧﺪﻣآ ﺖﺳﺪﺑ ﺮﺘﯿﻟ
.
ﻪﺑ رﻮﮐﺬﻣ شور ﺖﯿﻘﻓﻮﻣ رﻮﻃ
عاﻮﻧا يور ﺮﺑ يﺰﯿﻣآ
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ ﻪﻧﻮﻤﻧ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ مﺎﺠﻧا ﺮﯿﺷ يﺎﻫ
و ﺖﻓﺮﮔ
ﻪﻧﻮﻤﻧ ﺰﯿﻟﺎﻧآ نﺎﺸﻧ ﯽﻘﯿﻘﺣ يﺎﻫ
ﻪﻧﻮﻤﻧ ﻪﻤﻫ ﻪﮐ داد ﺮﯿﺷ يﺎﻫ
ياراد
ﯽﺘﻧآ هﺪﻧﺎﻤﯿﻗﺎﺑ هدوﺪﺤﻣ رد نآ راﺪﻘﻣ و هدﻮﺑ ﻦﯿﻠﮑﯾﺎﺳاﺮﺘﺗ ﮏﯿﺗﻮﯿﺑ
146 ﺎﺗ 319 ﯽﻠﯿﻣ رد مﺮﮔﻮﻧﺎﻧ ﺖﺳاﺮﯿﻐﺘﻣ ﺮﺘﯿﻟ
. ﺮﮕﯾد
ﯽﺘﻧآ ﮏﯿﺗﻮﯿﺑ ﻪﻧﻮﻤﻧ رد ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ ﺪﺸﻧ ﺖﻓﺎﯾ ﺎﻫ
.
4 - ﻊﻓﺎﻨﻣ ضرﺎﻌﺗ
ﺪﻧراﺪﻧ ﯽﻌﻓﺎﻨﻣ ضرﺎﻌﺗ ﻪﻧﻮﮕﭽﯿﻫ نﺎﮔﺪﻨﺴﯾﻮﻧ .
5 - ﻊﺑﺎﻨﻣ
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Application of a new extraction method coupled to high
performance liquid chromatography for tetracyclines monitoring in cow milk
Rasi, H. 1, AfsharMogaddam, M. 2,3 , Khandaghi, J. 4*
1. M.Sc Graduate of Food Science and Technology, Sarab Branch, Islamic Azad University, Sarab, Iran.
2. Assistant Professor of Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3. Assistant Professor of Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4. Assistant Professor of Department of Food Science and Technology, Sarab Branch, Islamic Azad University, Sarab, Iran.
ABSTRACT ARTIClE INFO
Article History:
Received 2020/ 10/ 19 Accepted 2021/ 03/ 07
Keywords:
Tetracycline, Milk,
Dispersive solid-phase micro-extraction, HPLC.
Excessive use of antibiotics in the production of livestock has led to the accumulation of antibiotic residues in various livestock products such as meat, milk and egg. Consumption of foods containing antibiotics leads to problems such as increased antibiotic resistance and allergies. For this reason, monitoring residual antibiotics, especially in high- consumption and beneficial food products such as milk and dairy products, is of particular importance. In this research, a dispersive solid-phase micro-extraction method for the extraction of tetracycline, oxytetracycline, chlortetracycline and doxycycline antibiotics from 50 milk samples and their quantitative measurement by HPLC was developed. For this purpose, the effect of various parameters affecting the extraction efficiency such as the volume of the precipitating agent, the type and amount of solid adsorbent, ionic strength, aeration time and flow rate, type and amount of surfactant and type and volume of elution solvent were optimized. The results showed that all milk samples contained tetracycline residue and its concentration varied in the range of 146 to 319 ng / ml. No other antibiotics were found in the samples. The advantages of the proposed extraction method include high separation power and the possibility of analyzing a mixture of highly sensitive analytes, so that under optimal conditions, recoveries of the proposed method ranging from 80 to 91%, the LOD were in the range of 0.17-0.33 ng / ml and the linear range was in the range of 0.63-2000 ng / ml.
DOI: 10.52547/fsct.18.04.27
*Corresponding Author E-Mail:
Iranian Journal of Food Science and Technology
Homepage: www.fsct.modares.ir
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