يﺎﻫﻪﭽﻫﺎﻴﮔﻲﻜﻳژﻮﻟﻮﻓرﻮﻣتﺎﻔﺻﻲﺧﺮﺑﺮﺑ ﭗﻴﺗﻮﻧژﺮﺛاﻲﺳرﺮﺑ ﻪﺸﻴﺷنورد
يا نﺎﻴﺑﻦﻳﺮﻴﺷ
ﻦﻳﺪﺑﺎﻋرﻮﭘﻦﻴﻣا
،1
ﻼﻋ ء ﭻﻴﻳﺎﻧدﺮﻛﻦﻳﺪﻟا و2
ﻲﻤﻴﻫاﺮﺑاﻲﻀﺗﺮﻣ
3
1 ﺪﻫﺎﺷهﺎﮕﺸﻧاد،يزروﺎﺸﻛيژﻮﻟﻮﻨﻜﺗﻮﻴﺑﺪﺷراﻲﺳﺎﻨﺷرﺎﻛ،ﻮﺠﺸﻧاد -
2 ﺪﻫﺎﺷهﺎﮕﺸﻧاد،يژﻮﻟﻮﻨﻜﺗﻮﻴﺑﺶﻳاﺮﮔتﺎﺗﺎﺒﻧحﻼﺻاﻲﺼﺼﺨﻧيﺮﺘﻛد،رﺎﻳدﺎﺘﺳا -
3 ﺎﺘﺳا -
يزروﺎﺸﻛيژﻮﻟﻮﻨﻜﺗﻮﻴﺑهﺪﻜﺸﻫوﮋﭘ،ﻲﻟﻮﻜﻟﻮﻣﺖﺴﻳزوﻚﻴﺘﻧژﻲﺳﺪﻨﻬﻣﻲﺼﺼﺨﺗيﺮﺘﻛد،رﺎﻳد [email protected]
:هﺪﻴﻜﭼ ﺷ ﻴﺮ ﻳ ﻦ ﺑﻴ نﺎ Glycyrrhiza glabra L ) ﻛ زاﻲﻌﻴﺳو ﻒﻴﻃ ياراد ﻪﻛ هدﻮﺑ ﻲﻳوراد نﺎﻫﺎﻴﮔ ﻦﻳﺮﺘﻤﻬﻣ ﻪﻠﻤﺟ زا (
ﻫدﺮﺑرﺎ رد ﺎ
ﺮﮔﻲﻣ ﺮﻴﺜﻜﺗﻲﺸﻳورشور ﻪﺑﺎﺗﺪﻤﻋهﺎﻴﮔﻦﻳا .ﺪﺷﺎﺑ ﻲﻣﻲﺘﺷاﺪﻬﺑ وﻲﺸﻳارآ ،ﻲﻳاﺬﻏ،ﻲﻳوراد ﻒﻠﺘﺨﻣﻊﻳﺎﻨﺻ ﻜﺗ .دد
ﻚﻴﻨ ﺖﺸﻛ
ﻨﻋﻪﺑﺖﻓﺎﺑ ﻮا
ﺮﻴﺜﻜﺗردﺪﻨﻤﺗرﺪﻗيراﺰﺑان هﻮﺒﻧا
ﺮﺗﺮﺑ يﺎﻫﭗﻴﺗﻮﻧژ .دﻮﺷ ﻲﻣﻪﺘﺧﺎﻨﺷ
ژﻞﺑﺎﻘﺘﻣﺮﺛا دﻮﺟو ﺤﻣو ﭗﻴﺗﻮﻧ
ﺮﺑ ﺖﺸﻛﻂﻴ
ﺪﺷ هداد نﺎﺸﻧ ﻒﻠﺘﺨﻣ نﺎﻫﺎﻴﮔ ﻲﻳاززﺎﺑ ﻣ تﺎﻴﺻﻮﺼﺧ ﺮﺑ ﭗﻴﺗﻮﻧژ ﺮﻴﺛﺎﺗ ﻲﺳرﺮﺑ فﺪﻫ ﺎﺑ ﻖﻴﻘﺤﺗ ﻦﻳا اﺬﻟ .ﺖﺳا ه
ﻮﻟﻮﻓرﻮ ﻲﻜﻳژ
رﺎﻬﭼ ﻲﺳرﺮﺑﻦﻳارد .ﺖﺳاهﺪﺷمﺎﺠﻧانﺎﻴﺑﻦﻳﺮﻴﺷيا ﻪﺸﻴﺷنوردﺖﺸﻛﻂﻳاﺮﺷردﻪﺘﻓﺎﻳﺪﺷرﻲﻳﺎﻫ ﻪﭽﻫﺎﻴﮔ ژ
ﭗﻴﺗﻮﻧ ﻣ توﺎﻔﺘ
نﺎﺳاﺮﺧ ،ﺪﻤﺣاﺮﻳﻮﺑ و ﻪﻳﻮﻠﻴﻜﻬﻛ،نﺎﺘﺴﻠﮔيﺎﻫ نﺎﺘﺳا زاهﺪﺷيروآ ﻊﻤﺟ يﺎﻫ ﺖﻴﻌﻤﺟزا يﻮﺿر
د ﻪﻛ مﻼﻳا و ﻲﺳرﺮﺑ ر
يﺎﻫ
ﺳاﻪﺘﻓﺮﮔراﺮﻗﻲﺳرﺮﺑدرﻮﻣ،ﺪﻧاهدﻮﺑﻲﻟﻮﻜﻟﻮﻣوﻲﻳﺎﻴﻤﻴﺷﻮﺘﻴﻓ،ﻲﻜﻳژﻮﻟﻮﻓﻮﻣيﺎﻫتوﺎﻔﺗيارادﻲﺗﺎﻣﺪﻘﻣ ﺘﺑا .ﺖ
ﻤﻧﺰﻳراﺪ يﺎﻫﻪﻧﻮ
ﺖﺸﻛ ﻂﻴﺤﻣ رد ﺎﻫ ﭗﻴﺗﻮﻧژ ﻦﻳا زا هﺮﮔ ﻚﺗ
MS
ﺪﺷر يهﺪﻨﻨﻛ ﻢﻴﻄﻨﺗ هدﺎﻣ ودهاﺮﻤﻫ ﻪﺑ 6
ﺰﻨﺑ- ﻳ ﻞ ﻣآ ﻴ رﻮﭘﻮﻨ ﻳ ﻦ و ﻛﻴ ﺘﻨﻴ ﻦ رد
نوردﻂﻳاﺮﺷ هرودﻚﻳردنﺎﻫﺎﻴﮔﺐﺳﺎﻨﻣﺪﺷرزاﺪﻌﺑ .ﺪﻧﺪﺷهدادﺖﺸﻛياﻪﺸﻴﺷ
رﻮﻣﻒﻠﺘﺨﻣتﺎﻔﺻ،هزور90 راﺮﻗﻲﺑﺎﻳزراد
ﻪﻛ داد نﺎﺸﻧ ﺎﻫ هداد ﻦﻴﮕﻧﺎﻴﻣ ﻪﺴﻳﺎﻘﻣ .ﺖﻓﺮﮔ تﺎﻔﺻ ﺮﻈﻧ زاﻲﺳرﺮﺑ درﻮﻣ يﺎﻫ ﭗﻴﺗﻮﻧژ ﻦﻴﺑ
ﻪﻗﺎﺳ لﻮﻃ
، اﺪﻌﺗ ﮕﻧﺎﻴﻣ د هﺮ لﻮﻃ ،
هﺮﮕﻧﺎﻴﻣ
، ﻨﻌﻣتوﺎﻔﺗ گﺮﺑ داﺪﻌﺗ و ﻪﺸﻳرداﺪﻌﺗ ،ﻪﺸﻳرلﻮﻃ ) راد ﻲ
p≤0.05%
.ﺖﺷاد دﻮﺟو ( نﺎﺘﺴﻠﮔ ﭗﻴﺗﻮﻧژ
ﺎﺑ ﺐﻴﺗﺮﺗﻪﺑ 4
ﻦﻴﮕﻧﺎﻴﻣ 33 / و10 ﭗﻴﺗﻮﻧژﺮﮕﻳدﻪﺑﺖﺒﺴﻧيﺮﺘﺸﻴﺑﺪﺷرزاهﺮﮕﻧﺎﻴﻣداﺪﻌﺗوﻪﻗﺎﺳلﻮﻃياﺮﺑﺮﺘﻣﻲﺘﻧﺎﺳ11 ﻫ
ﺑرادرﻮﺧﺮﺑﺎ .دﻮ
لﻮﻃ
و ﻪﺸﻳر داﺪﻌﺗ ،ﻪﺸﻳر مﻼﻳا ﭗﻴﺗﻮﻧژ گﺮﺑ داﺪﻌﺗ
ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ ﺐﻴﺗﺮﺗ ﻪﺑ 17 83
/
،ﺮﺘﻣ ﻲﺘﻧﺎﺳ 1 و 11
ﻋ 34 دﺪ د ﺎﺑ ﻪﺴﻳﺎﻘﻣ ر ﺮﮕﻳد
ﺎﻫﭗﻴﺗﻮﻧژ يﺮﺗﺮﺑ
ﺖﺷاديرادﻲﻨﻌﻣ .
:يﺪﻴﻠﻛيﺎﻫهژاو
،نﺎﻴﺑﻦﻳﺮﻴﺷ ﺖﺸﻛﻂﻴﺤﻣ،ﻪﻧﻮﻤﻧﺰﻳر،ياﻪﺸﻴﺷنورد
MS
The effect of genotype on morphological characteristics of licorice (Glycyrrhiza glabra L.) plantlets in in vitro condition
,Morteza ebrahimi3
Alaedin kordenaeej2
, Amin pourabedin1
1-Msc Student of Agriculture, Shahed University, Tehran, Iran 2- Faculty Member of Agriculture, Shahed University, Tehran, Iran
3-Faculty Member of Agriculture Biotechnology Research Institute of Iran, Esfahan, Iran
Abstract:
Licorice (Glycyrrhiza glabra L) is the most important medicinal plants with a wide range of applications in various industries such as pharmaceutical, food, cosmetics and healthcares. This plant is propagated mainly by vegetative approaches. Tissue culture technique is known as as a powerful tool for mass propagation of superior genotypes. It has been shown that the genotype and medium have interaction on plant regeneration. Therefore, this study aimed to evaluate the effect of genotype on the morphological characteristics of licorice in vitro grown seedlings. In this assay, four different genotypes of the populations collected from Golestan, Kohgiluyeh Boyer Ahmad, Khorasan and Ilam provinces with morphological, molecular and phytochemical differences were studied. First the single node explants of these genotypes were cultured on MS medium containing a combination of two growth regulators 6-Bnzylamynvpvryn and kinetin. Various parameters were recorded after 90-days of culture, when the appropriate growth was achieved. Mean comparison showed that there were significant differences (p≤0.05%) among the genotypes for the shoot length, number of internodes, internode length, root length, number of roots and leaves. Among the genotypes, the Golestan 4 was the fast growing one , with an average of 33.10 and 11 cm for the stems length and number of internodes, respectively. The root length, root number and leaves number of the Elam 17 were significantly superior compared with other genotypes, with 1.83 cm, 11 and 34, respectively.
Keywords: licorice, in vitro, explants, MS medium
:ﻪﻣﺪﻘﻣ
ﮔ
،ﻲﻤﻠﻋﺖﺧﺎﻨﺷترﻮﺻردﻪﻛﺪﻨﺘﺴﻫﻲﻌﻴﺒﻃﻊﺑﺎﻨﻣﻊﻴﺳويهﺮﺘﺴﮔردﺪﻨﻤﺷزراﻊﺑﺎﻨﻣزاﻲﻜﻳﻲﻳورادنﺎﻫﺎﻴ
،ﺖـﺸﻛ ﺗ وﻪﻌـﺳﻮ
ﺪﻨﺷﺎﺑﻪﺘﺷادﻲﺘﻔﻧﺮﻴﻏتاردﺎﺻوﻲﻳازلﺎﻐﺘﺷا،ﻪﻌﻣﺎﺟﺖﻣﻼﺳردﻲﻤﻬﻣﺶﻘﻧﺪﻨﻧاﻮﺗﻲﻣيرادﺮﺑهﺮﻬﺑ )
1 ﺳاﺮﺑ .( يﺎـﻫدروآﺮﺑسﺎ
يﺎﻫرازﺎﺑشزراﺮﻴﺧايﺎﻫلﺎﺳردﻪﺘﻓﺮﮔترﻮﺻ رﺎـﺑهراﻮـﻤﻫنآزاﻞﺻﺎﺣيﺎﻫهدروآﺮﻓوﻲﻳورادنﺎﻫﺎﻴﮔﻲﻧﺎﻬﺟ
ﻞـﺑﺎﻗﺪـﺷ
) ﺖﺳاهدﻮﺑهاﺮﻤﻫﻲﻬﺟﻮﺗ 5
لﺎـﺳردﻲﻳورادنﺎﻫﺎﻴﮔﻲﻧﺎﻬﺟرازﺎﺑشزرا،ﺪﻫدﻲﻣنﺎﺸﻧدﻮﺟﻮﻣيﺎﻫرﺎﻣآ .(
ـﺑ2002 ﺪـﺷرﺎ 2 / 6
زاﺶﻴﺑﻪﺑنآزاﺶﻴﭘلﺎﺳﻪﺑﺖﺒﺴﻧيﺪﺻرد 7
/ رﻻددرﺎﻴﻠﻴﻣ13 ﺪﻳدﺮﮔﻎﻟﺎﺑ
) 5 نﺎـﻴﻣﻦﻳارد .( ترﺎـﺠﺗشزرا
ـﻬﺟ ﻲﻧﺎ ـﺷ ﻴﺮ ﻳ ﻦ
ﺑﻴ نﺎ لﺎﺳرد
،2007 دوﺪﺣ ﻣﻴ42 ﻠﻴ نﻮ ﺎﻜﻳﺮﻣآرﻻد ﻤﺨﺗ
ﻴ ﻦ ﺪﺷهدز ﺖﺳاه ﻴﺑردهﺎﻴﮔﻦﻳاﺖﻴﻤﻫازانﺎﺸﻧﻦﻳاﻪﻛ ﻲﻳورادنﺎﻫﺎﻴﮔﻦ
دراد ) 4 ( .
ﺷ ﻴﺮ ﻳ ﻦ ﺑﻴ نﺎ
Glycyrrhiza glabra L) هداﻮﻧﺎﺧﻪﺑﻖﻠﻌﺘﻣ (
ي ﺖـﺳاﺎـﻫمﻮـﮕﻟ و
ﻦﻳﺮـﺘﻤﻬﻣزاﻲـﻜﻳ ـﻣ
ﻮ هﺮﺛﻮـﻣدا ي
نآ ﻳ ـﻨﻌ ﻲ
ﻴﻠﮔ ﺴ ﻦﻳﺰﻳﺮﻴ
Glycyrrhizin) ناﻮﻨﻌﺑهدﺮﺘﺴﮔرﻮﻄﺑ(
ﻳ ﻚ ﺷ ﻴﺮ ﻳ ﻦ ﺒﻃهﺪﻨﻨﻛ ﻴﻌ ﻲ ﻨﭽﻤﻫو ﻴ ﻦ ﺻﺎﺧﺮﻃﺎﺨﺑ ﻴ
ﺖ ﺑﺎـﻬﺘﻟاﺪﺿ ﻲ
ﻆﻓﺎـﺤﻣو
ﮔﺪﻨﻨﻛ ﻲ ﺪﺒﻛزا ناﻮﻨﻌﺑ ﻳ ﻚ هدﺎﻣ وراد ﻲﻳ .دﻮﺷﻲﻣهدﺎﻔﺘﺳا اﺮﺑهوﻼﻋ
ﻳ
،ﻦ ﺷتﺎﻘﺘﺸﻣ ﻴﺮ
ﻳ ﻦ ﺑﻴ نﺎ ارآدراﻮـﻣناﻮـﻨﻌﺑ ﻳ
ـﺸ ﻲ
، ـﻧدوﺰﻓا ﻲ
،ﺎﻫاﺬﻏ ﺷوﻮﻛﺎﺒﻨﺗهﺪﻨﻫدﻢﻌﻃ ﻴﺮ
ﻳ ﻦ اﺬﻏهﺪﻨﻨﻛ ﻲﻳ
درادﻲﻳاهدﺮﺘﺴﮔدﺮﺑرﺎﻛ )
4 رﻮـﺸﻛردنﺎـﻴﺑﻦﻳﺮﻴـﺷيﻻﺎﺑﺪﻴﻟﻮﺗﺖﻴﻓﺮﻇ.(
) 18
ﺪﺼﻴﺳوراﺰﻫ و
ردلﺎﺳردﻦﺗهﺎﺠﻨﭘ تاردﺎـﺻناﺰـﻴﻣﻲـﺸﻫﺎﻛﺪـﻧورﺎـﺑلﺎـﺣﻦﻳاﺎﺑﻪﻛﺖﺳاﻪﺟﻮﺗﻞﺑﺎﻗ يﺮﻣا (ﻪﻧﺎﺧرﺎﻛ14
زاﺶﻫﺎﻛ) لﺎﺳردرﻻدنﻮﻴﻠﻴﻣ44
ﻪﺑ90 لﺎﺳردرﻻدنﻮﻴﻠﻴﻣ33 93
.ﺖﺳاهدﻮﺑهاﺮﻤﻫ ( ﺮﻣاﻦﻳاﻞﻳﻻدزاﻲﻜﻳ
ﻪﺑﻲﺳﺮﺘﺳدمﺪﻋ
هﺎﻴﮔﻦﻳاﻪﻴﻟواهدﺎﻣ ﺪﺷﺎﺑﻲﻣ
نﺎﻴﺑﻦﻳﺮﻴﺷﻪﻴﻟوايهدﺎﻣﺪﻴﻟﻮﺗﺶﻳاﺰﻓايﻪﻨﻴﻣزردﻢﻬﻣيﺎﻫرﺎﻜﻫارزا . ﻪﺑﻚﻤﻛ
ﻲﻋارز ﻦـﻳايزﺎﺳ
يﺎﻴﺣاوهﺎﻴﮔ ﻲﻌﻴﺒﻃيﺎﻫهﺎﮕﺸﻳوروﻲﺿارا
نآ ﺖﺳا يﺎـﻫﻪـﭽﻫﺎﻴﮔردﻲﻜﻳزﻮﻟﻮﻓرﻮﻣتﺎﻔﺻﺮﺑﭗﻴﺗﻮﻧژتاﺮﻴﺛﺎﺗيهزﻮﺣرد .
ﺖـﺳاهﺪـﺸﻧمﺎـﺠﻧانﺎﻴﺑﻦﻳﺮﻴﺷهﺎﻴﮔدرﻮﻣردﻲﺗﺎﻘﻴﻘﺤﺗياﻪﺸﻴﺷنوردﺖﺸﻛزاﻞﺻﺎﺣ )
6 ﺮـﮕﻳدنﺎـﻫﺎﻴﮔردﻪـﻛﺪـﻨﭼﺮـﻫ .(
.دراددﻮﺟوﻪﺑﺎﺸﻣتﺎﻘﻴﻘﺤﺗ ) نارﺎﻜﻤﻫوﻲﺒﻟﺎﻃ
،(1394 ﻲﺳرﺮﺑ سرﺮﻳدوسردوزﻢﻗررﺎﻬﭼ ﻲﻨﻴﻣزﺐﻴﺳهﺎﻴﮔ
تﺎﻔـﺻﺮـﻈﻧزا
ﻲﻜﻳژﻮﻟﻮﻓرﻮﻣ يرادﻲﻨﻌﻣتﺎﻓﻼﺘﺧاﻪﺑ
ﺪﻨﺘﻓﺎﻳﺖﺳد رد.
ﻢﻗرنﻮﻟﻮﺘﺳاداﺪﻌﺗوهﺎﻴﮔﺮﻫردﺐﻛﺮﻣگﺮﺑداﺪﻌﺗ،ﻪﻗﺎﺳلﻮﻃتﺎﻔﺻ
هﺪﻏضﺮﻋوداﺪﻌﺗتﺎﻔﺻردواﺪﻨﻳرآ ﻢﻗرهﺪﻏنزوولﻮﻃ،ﻪﻧاﻮﺟداﺪﻌﺗ،ﺎﻳﺮﮔآ ﻢﻗرهﺪﻏباﻮﺧﻦﺘﺴﻜﺷيﺎﻫزورداﺪﻌﺗ،ﺎﻨﻴﺗﺎﺳ ﺪﻧدادنﺎﺸﻧيﺮﺗﺮﺑﺮﮕﻳﺪﻜﻳﺎﺑﻪﺴﻳﺎﻘﻣردﻪﻨﺗﺎﺳﻢﻗر )
8 (
Doctrinal .
Marylise
نارﺎﻜﻤﻫو
) ردﭗﻴﺗﻮﻧژتاﺮﺛاﻲﺳرﺮﺑﻪﺑ، (1988
ﭗﻴﺗﻮﻧژﺞﻨﭘﻲﺳرﺮﺑﺎﺑﺎﻬﻧآﻖﻴﻘﺤﺗﻦﻳارد .ﺪﻨﺘﺧادﺮﭘرﺪﻨﻐﭼهﺎﻴﮔ
,s5
,s4
,s3
,s2
s1
ﭗـﻴﺗﻮﻧژﻪـﻛﺪـﻨﺘﻓﺎﻳﺖـﺳدﻪـﺠﻴﺘﻧﻦـﻳاﻪﺑ
s3
ﺎـﺑ
دﺮﻜﻠﻤﻋﺺﺧﺎﺷ 81
% )ﺖﺳاﻪﺘﺷادنﺎﻴﻣﻦﻳاردارﺪﺷرناﺰﻴﻣﻦﻳﺮﺘﺸﻴﺑ 3
Christopher.(
ﭗﻴﺗﻮﻧژتاﺮﻴﺛﺎﺗﻲﺳرﺮﺑﺎﺑنارﺎﻜﻤﻫو
ﺰﻴﻣﺮﺑ ﭗـﻴﺗﻮﻧژﻪﺳﻲﺳرﺮﺑﺎﺑﺎﻬﻧآ.ﺪﻨﺘﻓﺎﻳﺖﺳدﻪﻨﻴﻣزﻦﻳارديرادﻲﻨﻌﻣتﺎﻓﻼﺘﺧاﻪﺑﺰﻣﺮﻗﻞﻔﻠﻓهﺎﻴﮔردﺪﺷرنا 260553
C.
annuum, C. praetennissum PI 342947, C. baccatum PI ﺖـﺳدﻪﻗﺎـﺳداﺪﻌﺗﺖﻔﺻرديرادﻲﻨﻌﻣفﻼﺘﺧاﻪﺑ
ﭗﻴﺗﻮﻧژنﺎﻴﻣﻦﻳارد .ﺪﻨﺘﻓﺎﻳ C. praetennissum PI 342947
ﺸﻴﺑ )دادصﺎﺼﺘﺧادﻮﺧﻪﺑارﺖﻔﺻﻦﻳاراﺪﻘﻣﻦﻳﺮﺘ 2
.(
ﮔﻲﻣﻮﺑيﺎﻫﭗﻴﺗﻮﻧژردتﺎﻔﺻﻦﻳاﺮﺑﭗﻴﺗﻮﻧژﺮﻴﺛﺎﺗوﻲﻜﻳژﻮﻟﻮﻓرﻮﻣتﺎﻔﺻﻲﺳرﺮﺑفﺪﻫﺎﺑﺰﻴﻧ هﺪﺷمﺎﺠﻧاﻖﻴﻘﺤﺗ ﻦﻳﺮﻴـﺷهﺎـﻴ
.ﺖﺳاهﺪﺷمﺎﺠﻧانﺎﻴﺑ
:ﺎﻫشوروداﻮﻣ
ﻲﻫﺎﻴﮔداﻮﻣﻪﻴﻬﺗ
ﻔـﺻاردﻊﻗاو،ﻪـﻳﻮﻧﺎﺛيﺎﻫﺖﻴﻟﻮﺑﺎﺘﻣهﺪﻜﺸﻫوﮋﭘردﺮﺿﺎﺣﺶﻫوﮋﭘ لﺎـﺳرد،نﺎﻬ
1396 - ﺮﮔاﺮـﺟا1395 ﭗـﻴﺗﻮﻧژرﺎـﻬﭼزا .ﺪـﻳد
ﻒﻠﺘﺨﻣ نﺎﺘـﺴﻠﮔﻞﻣﺎـﺷ (ﺖـﺳاهﺪـﺸﻧهدادنﺎﺸﻧتﺎﻋﻼﻃا) ناﺮﻳانﺎﻴﺑﻦﻳﺮﻴﺷيﺎﻫﺖﻴﻌﻤﺟﻲﺗﺎﻣﺪﻘﻣﻲﺳرﺮﺑزاﻞﺻﺎﺣ 4
ﻊـﻤﺟ)
نﺎﺘﺳازاهﺪﺷيروآ (نﺎﻴﺳرﺎﻓ،ﺶﻜﻴﻟﺎﮔ،نﺎﺘﺴﻠﮔ
جﻮﺳﺎﻳ،
،ﺪﻤﺣاﺮﻳﻮﺑوﻪﻳﻮﻠﻴﻜﻬﻛنﺎﺘﺳا)1 ﻮﺑنﺎﺘﺳﺮﻬﺷ
ﻳﺮ ﺪﻤﺣا
، ﻣ ﻮـﮔﺮﮔﻪـﻘﻄﻨ د
(
نﺎﺳاﺮﺧ،
نﺎﺘﺳا)8 (خﺮﺳهد ،دﺎﺑآﻚﻠﻣ،يﻮﺿرنﺎﺳاﺮﺧ مﻼﻳاو
،مﻼﻳانﺎﺘﺳا)17 ﻔﻌﺟﺎﺘﺳور،ﺮﻬﺷهردنﺎﺘﺳﺮﻬﺷ
(نﺎﻜﻤﭼ،دﺎﺑآﺮ
رددﻮﺟﻮﻣ ـﻧﻮﻤﻧﺰـﻳرزاﻲﺳرﺮﺑﻦﻳارد .ﺪﺷيرادﺮﺑﻪﻧﻮﻤﻧ،(نﺎﻬﻔﺻا) ﻪﻳﻮﻧﺎﺛيﺎﻫﺖﻴﻟﻮﺑﺎﺘﻣهﺪﻜﺸﻫوﮋﭘنﻮﻴﺴﻜﻠﻛ
ﻪـﻧاﻮﺟيﺎـﻫﻪ
ﺷهدﺎﻔﺘﺳا ﻲﺒﻧﺎﺟ ﻞﻜﻟاﺎﺑاﺪﺘﺑاﺎﻫﻪﻧﻮﻤﻧﺰﻳر .ﺪ
70 تﺪﻣﻪﺑ% ﻢﻳﺪﺳﺖﻳﺮﻠﻛﻮﭙﻴﻫﺲﭙﺳوﻪﻴﻧﺎﺛ30
5 / 1 ﭼيوﺎﺣ% ﻦﻴﻳﻮـﺗهﺮـﻄﻗﺪﻨ
تﺪﻣﻪﺑ20 15 .ﺪﻧﺪﺷﻮﺸﺘﺴﺷﻞﻳﺮﺘﺳاﺮﻄﻘﻣبآﺎﺑﻪﺒﺗﺮﻣﻪﺳﻞﻗاﺪﺣوﻲﻧﻮﻔﻋﺪﺿﻪﻘﻴﻗد
ﻪﻧﻮﻤﻧﺰﻳرﺖﺸﻛ
ﻴﺤﻣردقﻮﻓيﺎﻫﭗﻴﺗﻮﻧژزاهﺪﺷﻲﻧﻮﻔﻋﺪﺿﻲﺒﻧﺎﺟﻪﻧاﻮﺟيﺎﻫﻪﻧﻮﻤﻧﺰﻳر :راﺮﻘﺘﺳا ﻪﻳﺎﭘﺖﺸﻛﻂ
MS1
ﻢﻴـﻈﻨﺗزاﻲـﺒﻴﻛﺮﺗيوﺎﺣ
ﺪﺷريﺎﻫهﺪﻨﻨﻛ )
mg/l 1.5 ( BAP2
) ، mg/l 0.5 ( Kin3
)، mg/l 0.1 ( IBA4
)و mg/l 0.5 ( 35
هاﺮـﻤﻫﻪـﺑGA مﺮـﮔ30
وزرﺎﻛﺎﺳﺮﺘﻴﻟرد ورﺎﮔآﺮﺘﻴﻟردمﺮﮔ6
ﺮﺑاﺮﺑpH 8 / ـﺑﻪـﺘﻓﺮﮔراﺮـﻗﻲﺑﺎﻳزرادرﻮﻣويزﺎﺳﻪﻨﻴﻬﺑاﺰﺠﻣﻲﻘﻴﻘﺤﺗردﻪﻛ5
،دﻮ ﺖـﺸﻛ
يﺎﻣدردنوﺮﺘﺳﻂﻳاﺮﺷﺖﺤﺗﺪﺷرﻚﻗﺎﺗاردﺎﻫﻪﭽﻫﺎﻴﮔ .ﺪﻧﺪﺷ
±2 ﻲﻳﺎﻨـﺷورلﻮـﻃ وداﺮﮔﻲﺘﻧﺎﺳيﻪﺟرد25 ﺖﻋﺎـﺳ16
ﺎـﺑ
تﺪﺷ رﻮﻧ ﺲﻛﻮﻟ3000
و ترﻮﺻﻪﺑﺎﻫﻪﭽﻫﺎﻴﮔ،ﻲﺒﻧﺎﺟيﺎﻫﻪﻧاﻮﺟﺪﺷرزاﺲﭘ .ﺪﻨﺘﻓﺮﮔراﺮﻗﻲﻜﻳرﺎﺗﺖﻋﺎﺳ8 ﻪﺑ
ﺮﻴﺜﻜﺗﻂﻴﺤﻣ
ﻞﻘﺘﻨﻣ .ﺪﻧﺪﺷ
1.Murashige and Skoog medium
2 . 6-Benzylaminopurine
3 . Kinetin
4 . indole-3-acetic acid
:ﺮﻴﺜﻜﺗ ﺎﻳﺪﺷرﻲﺒﻧﺎﺟيﺎﻫﻪﻧاﻮﺟ ﻞﺻﺎﺣﻪﺘﻓ
يﻪﻳﺎﭘﺖﺸﻛﻂﻴﺤﻣ ﻪﺑ،راﺮﻘﺘﺳازا ﺪـﻨﻨﻛﻢﻴﻄﻨﺗزاﻲﺒﻴﻛﺮﺗيوﺎﺣMS
ﺪـﺷريﺎـﻫه
BAP (1.5 mg/l) Kin (0.5 mg/l) و
.ﺪﻨﺘﻓﺎﻳلﺎﻘﺘﻧا نﺎﻣزيﻪﻠﺻﺎﻓﻪﺑﻪﻠﺣﺮﻣﻪﺳﻲﻃرد
40 .ﺪﻧﺪـﺷﺖـﺸﻛاوزور ﺮـﺷ
ﻂﻳا
.دﻮﺑﻞﺒﻗﻪﻠﺣﺮﻣﺪﻨﻧﺎﻤﻫﺎﻫﻪﻧﻮﻤﻧيراﺪﻬﮕﻧ ﻳﺎﭘرد
يهرودنﺎ زور90
يرادﺮﺑﻪﻧﻮﻤﻧ،ه ﻮـﻟﻮﻓرﻮﻣتﺎﻔﺻﻲﺳرﺮﺑو
ﻪـﻠﻤﺟزاﻲﻜﻳژ
)ﻪﻗﺎﺳلﻮﻃ
،گﺮﺑداﺪﻌﺗ،(cm
،هﺮﮕﻧﺎﻴﻣلﻮﻃ
،هﺮﮕﻧﺎﻴﻣداﺪﻌﺗ ﻪﺸﻳرداﺪﻌﺗوﻪﺸﻳرلﻮﻃ
ﺖﻓﺮﮔراﺮﻗﻲﺑﺎﻳزرادرﻮﻣ .
ﺎﻫهداديرﺎﻣآﻪﻳﺰﺠﺗوﻲﺸﻳﺎﻣزآحﺮﻃ
ﺎﺠﻧاراﺮﻜﺗﻪﺳردﻲﻓدﺎﺼﺗﻼﻣﺎﻛيرﺎﻣآحﺮﻃﺐﻟﺎﻗردﻞﻳرﻮﺘﻛﺎﻓترﻮﺻﻪﺑﺶﻳﺎﻣزآﻦﻳا .ﺖﻓﺮﻳﺬﭘم
يرﺎﻣآﺰﻴﻟﺎﻧآ هدﺎﻔﺘﺳاﺎﺑ
مﺮﻧزا
راﺰﻓا 1 /
SAS 9 راﺰﻓامﺮﻧﺎﺑﺎﻫرادﻮﻤﻧﻢﻴﺳﺮﺗو Excel
مﺎﺠﻧا ﺷ ﺪ .
:ﺚﺤﺑوﺞﻳﺎﺘﻧ
يﺎﻫهدادﺲﻧﺎﻳراوﻪﻳﺰﺠﺗلوﺪﺟﺞﻳﺎﺘﻧﻪﺑﻪﺟﻮﺗﺎﺑ زاهﺪﺷيﺮﻴﮔهزاﺪﻧا
مﺎـﻗراردياﻪﺸﻴﺷنورديﺎﻫﻪﭽﻫﺎﻴﮔ ﺗ
،ﻲـﺳرﺮﺑ ﺖـﺤ
ﺳردتﺎﻔﺻﻲﻣﺎﻤﺗ لﺎﻤﺘﺣاﺢﻄ
ﻣﺪﺻرد5 رادﻲﻨﻌ رادﻮﻤﻧ) ﺪﻧدﻮﺑ 1
( .
لﻮﻃ:ب،ﺎﻫگﺮﺑداﺪﻌﺗ:ﻒﻟا.نﺎﻴﺑﻦﻳﺮﻴﺷﭗﻴﺗﻮﻧژرﺎﻬﭼﻲﻳاﻪﺸﻴﺷنورديﺎﻫﻪﭽﻫﺎﻴﮔﻦﻴﺑﻒﻠﺘﺨﻣﻲﻜﻳژﻮﻟﻮﻓﺮﻣيﺎﻫﺮﺘﻣارﺎﭘﻦﻴﮕﻧﺎﻴﻣﻪﺴﻳﺎﻘﻣ.1رادﻮﻤﻧ
،ﻪﻗﺎﺳ :ج
،هﺮﮕﻧﺎﻴﻣداﺪﻌﺗ :د
،هﺮﮕﻧﺎﻴﻣلﻮﻃ :و
،ﻪﺸﻳرداﺪﻌﺗ :ي
ﻪﺸﻳرلﻮﻃ ﺎﺑﺎﻫﻦﻴﮕﻧﺎﻴﻣﻪﺴﻳﺎﻘﻣ) . ﭼنﻮﻣزآزاهدﺎﻔﺘﺳا
ﻦﻜﻧادﻪﻨﻣادﺪﻨ P≤0.05)
ﻣ .(
ﻦﻴﮕﻧﺎﻴ
لﺎﻤﺘﺣاﺢﻄﺳردرادﻲﻨﻌﻣفﻼﺘﺧاكﺮﺘﺸﻣفوﺮﺣياراديﺎﻫ 5
% (ﺪﻧراﺪﻧ
و ب ﻒﻟا
د
ي
ج
نﺎﺘﺴﻠﮔ ﻪﻧﻮﻤﻧ،ﻪﻗﺎﺳ لﻮﻃ ﺖﻔﺻرد ﻪﻛ ﺪﻫد ﻲﻣنﺎﺸﻧ ﺎﻫهدادﻦﻴﮕﻧﺎﻴﻣ ﻪﺴﻳﺎﻘﻣ ﺞﻳﺎﺘﻧ ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ 4
33 / ﺳ 10 ﻦﻳﺮﺘﺸﻴﺑ ،ﺮﺘﻣ ﻲﺘﻧﺎ
ﻗراﺮﻳﺎﺳﻪﺑﺖﺒﺴﻧارﺪﺷر ناﺰﻴﻣ نﺎﺳاﺮﺧ ﭗﻴﺗﻮﻧژو ﻪﺘﺷادمﺎ
ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ8 33
/ ﺖﻔﺻﻦﻳا ناﺰﻴﻣﻦﻳﺮﺘﻤﻛ3 ر
رد .ﺪﻫدﻲﻣ نﺎﺸﻧا
مﻼﻳا گﺮﺑداﺪﻌﺗ ﺖﻔﺻ ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ17
نﺎﺳاﺮﺧو ﻦﻳﺮﺘﺸﻴﺑ،گﺮﺑ دﺪﻋ34 ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ8
33 / داﺪﻌﺗ ﻦﻳﺮﺘﻤﻛ14 ﻂﻳاﺮﺷ ردارگﺮﺑ
جﻮﺳﺎﻳ هﺮﮕﻧﺎﻴﻣ لﻮﻃ ﺺﺧﺎﺷ رد .ﺪﻧا هدﺮﻛ ﺪﻴﻟﻮﺗ ﻲﻳا ﻪﺸﻴﺷ نورد ﻧﺎﻴﻣ ﺎﺑ 1
ﻦﻴﮕ 67 / و ﻦﻳﺮﺘﺸﻴﺑ ﺮﺘﻣ ﻲﺘﻧﺎﺳ 1 نﺎﺳاﺮﺧ
ﺎﺑ 8
ﻦﻴﮕﻧﺎﻴﻣ 17 / ﻠﮔ ﻪﻧﻮﻤﻧ ﺰﻴﻧ هﺮﮕﻧﺎﻴﻣ داﺪﻌﺗ ﺖﻔﺻ رد ﻦﻴﮕﻧﺎﻴﻣ ﻪﺴﻳﺎﻘﻣ ﻲﺳرﺮﺑ ﺎﺑ.ﺪﻨﺘﺷاد ار راﺪﻘﻣ ﻦﻳﺮﻨﻤﻛ 1 نﺎﺘﺴ
ﺎﺑ 4 ﻣ ﻦﻴﮕﻧﺎﻴ 11
نﺎﺳاﺮﺧو داﺪﻌﺗ ﻦﻳﺮﺘﺸﻴﺑ ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ8
67 / ﻛﺪﻴﻟﻮﺗ ﺎﻫﭗﻴﺗﻮﻧژ ﺮﮕﻳد نﺎﻴﻣردارهﺮﮕﻧﺎﻴﻣ داﺪﻌﺗ ﻦﻳﺮﺘﻤﻛ3 ﻦﻴﺑ .ﺪﻧاهدﺮ
ﻴﺗﻮﻧژ يا ﭗ
) يراد ﻲﻨﻌﻣتوﺎﻔﺗ ﺰﻴﻧ ﻲﻳاز ﻪﺸﻳرﻪﺑ طﻮﺑﺮﻣتﺎﻔﺻ ﺮﻈﻧزا ﻲﺳﺮﺑ درﻮﻣ p≤0.05%
.ﺖﺷاددﻮﺟو ( جﻮﺳﺎﻳ
ﻴﻣ ﺎﺑ1 ﻦﻴﮕﻧﺎ 5 / 4
نﺎﺘﺴﻠﮔ و ﻦﻳﺮﺘﺸﻴﺑﺮﺘﻣ ﻲﺘﻧﺎﺳ ﻦﻴﮕﻧﺎﻴﻣ ﺎﺑ 4
4 داﺪﻌﺗ ﺺﺧﺎﺷرد .ﺪﻨﺘﺷادارﻪﺸﻳرلﻮﻃ ﻦﻳﺮﺘﻤﻛﺮﺘﻣ ﻲﺘﻧﺎﺳ ./ مﻼﻳا ﺰﻴﻧ ﻪﺸﻳر
ﺑ 17 ﺎ
ﻦﻴﮕﻧﺎﻴﻣ ﻮﺑ ﭗﻴﺗﻮﻧژﻦﻳﺮﺘﻬﺑدﺪﻋ11
) د رادﻮﻤﻧ 1 ﻲﺳرﺮﺑفﺪﻫﺎﺑﻪﻛ ﺮﺿﺎﺣﻖﻴﻘﺤﺗ .(
ﺮﺑ ﭗﻴﺗﻮﻧژ ﺮﻴﺛﺎﺗ تﺎﻴﺻﻮﺼﺧ
ﻲﻜﻳژﻮﻟﻮﻓرﻮﻣ
ﻳﺮﻴﺷ ﭗﻴﺗﻮﻧژ رﺎﻬﭼ رد يا ﻪﺸﻴﺷ نورد يﺎﻫ ﻪﭽﻫﺎﻴﮔ
،ﺖﺳا هﺪﺷ مﺎﺠﻧا نﺎﻴﺑ ﻦ هﺪﻨﻨﻛﺪﻴﻳﺎﺗ
ﻛ ﺖﺳا ﺐﻠﻄﻣ ﻦﻳا ي اﺮﺷ رد ﻪ
ﻂﻳ
نﺎﺴﻜﻳﻲﺸﻳور
، ارهﺎﻴﮔتﺎﻔﺻزايرﺎﻴﺴﺑﺪﻧاﻮﺗﻲﻣﭗﻴﺗﻮﻧژعﻮﻧ .ﺪﻫدراﺮﻗﺮﻴﺛﺎﺗﺖﺤﺗ
ﻪﺑ اﻮﺗﻲﻣﻲﻠﻛرﻮﻃ ﻮﻧژﺖﻔﮔ ن
يﺎﻫﭗﻴﺗ
داﻮﻣ ﻒﻠﺘﺨﻣحﻮﻄﺳﺰﺘﻨﺳ ﺎﺑﻒﻠﺘﺨﻣ
،داﻮﻣ ﻦﻳاﻦﻴﺑﺎﻣنزاﻮﺗوهدﻮﻤﻧ ﺎﻔﻳاارﻲﻤﻬﻣ ﺶﻘﻧﻲﻠﺧاد ﺪﺷر يﻮﮕﻟا
ر ﺗارﺪﺷ ﻲﻣ ﻦﻴﻴﻌ
ﺪﻨﻛ دﻮﺷﻲﻣﻞﺻﺎﺣﺖﺸﻛﻂﻴﺤﻣﻂﻳاﺮﺷﻪﺑﺖﺒﺴﻧﻲﻔﻠﺘﺨﻣيﺎﻫﺦﺳﺎﭘﻪﺠﻴﺘﻧردو )
8 .(
:ﻊﺑﺎﻨﻣ
1. Arya. A, Rathi. N, Arya I. D. (2009). "Micropropagation Protocol for Glycyrrhiza glabra L.
Phytomorphology". 59(1&2):71-76
2. Christopher. T, Rajam. M. V. (1995). "Effect of genotype, explant and medium on in vitro regeneration of red Pepper".Plant Cell, Tissue and Organ Culture 46: 2452.50
3. Doctrinal. M, Sangwan. R. J, Brigtte. S. (1987). "In vitro gynogenesis in Beta vulgaris L.: Effects of plant growth regulators, temperature, genotypes and season". Plant Cell, Tissue and Organ Culture.17:1-12 4. Hayashi. H. Sudo. H., (2009). "Economic importance of licorice". Plant Biotechnology 26:101-104 5. Kamali. H (2015). "Licorice outlines the opportunities and challenges facing the country".The fourth national congress on medicinal plants Country
6. Kashfi Bonab. A (2010). "Economic comparative advantage the cultivation and trade of medicinal plants in Iran and its value on world markets".Monthly Notices of the Iran Chamber.44:20-28
7. Oyunbileg. Y, Khorolrragchaa. A, Bayarlkhagva. D (2005). "Tissue culture and Micropropagation of Mongolian licorice".Mongolian Journal of Biological Sciences.3 (1):21-24
8. Talebi, M. Farahani, F. Taheri, B. (2016). "The effect of genotype on some morphological and biochemical traits of culture plantlets in vitro in the early and late potato cultivars under greenhouse conditions".
International conference on modern Research Results in sciences, Engineering and Technology: 1-12.
9. Thengane. S. R, Kulkarni. D. K, Krishnamurthy.K.V (1998). "Propagation of licorice (Glycyrrhiza Glabra L.) Through Shoot Tip and Nodal cultures". Society for In Vitro Biology.34:331-334
