ﺎﻨﻠﺼﻓﻭﺩ ﺏﻮﻨﺟ ﺐﻃ ﻪﻣ
ﺖﺴﻳﺯ ﻩﺪﻜﺸﻫﻭﮋﭘ ‐
ﺱﺭﺎﻓ ﺞﻴﻠﺧ ﻲﻜﺷﺰﭘ
ﺱﺭﺎﻓ ﺞﻴﻠﺧ ﻲﻧﻮﻔﻋ ﻭ ﻱﺮﻴﺴﻣﺮﮔ ﺐﻃ ﺕﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ ﺮﻬﺷﻮﺑ ﻲﻧﺎﻣﺭﺩ ﻲﺘﺷﺍﺪﻬﺑ ﺕﺎﻣﺪﺧ ﻭ ﻲﻜﺷﺰﭘ ﻡﻮﻠﻋ ﻩﺎﮕﺸﻧﺍﺩ
ﻝﺎﺳ ﻢﻫﺩ ﻩﺭﺎﻤﺷ ، ۱ ، ﻪﺤﻔﺻ ۸ ‐ ۱ ) ﺭﻮﻳﺮﻬﺷ ۱۳۸۶ (
ﻥﺍﺮﻳﺍ ،ﻥﺍﺮﻬﺗ ،ﻥﺍﺮﻬﺗ ﻩﺎﮕﺸﻧﺍﺩ ،ﻲﻤﻴﺷﻮﻴﺑ ﺶﺨﺑ ،ﮏﻳﺰﻴﻓﻮﻴﺑ ﻭ ﻲﻤﻴﺷﻮﻴﺑ ﺕﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ* ﺻ
ﻲﺘﺴﭘ ﻕﻭﺪﻨ ۱۳۱۸۴
‐ ۱۳۱۴۵
، ﻦﻔﻠﺗ : ۶۶۴۹۹۴۲۲ ‐
۰۲۱
[email protected] Email:
ﻦﻴﺌﺗﻭﺮﭘ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺮﻴﺛﺄﺗ ﻲﺳﺭﺮﺑ ﺮﺑ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﺯﺍ ﻩﺪﺷ ﺝﺍﺮﺨﺘﺳﺍ ﻱﺎﻫ
ﻪﺟﻮﺟ ﻦﻴﻨﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎﺸﻏ
ﺮﺘﻛﺩ ﻲﻫﺎﮔﺭﺎﺑ ﺭﺎﺸﻓﺍ ،۱
ﺮﺘﻛﺩ ﻲﻧﺎﮔﺩﺎﭼ ﻲﻧﺎﺑﺭ ﺍﺭﺬﻋ
* ۲
ﺮﮕﺸﻫﻭﮋﭘ ،ﻲﻤﻴﺷﻮﻴﺑ ﻱﺍﺮﺘﻛﺩ١
ﺱﺭﺎﻓ ﺞﻴﻠﺧ ﻲﻧﻮﻔﻋ ﻭ ﻱﺮﻴﺴﻣﺮﮔ ﺐﻃ ﺕﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ ،
ﺮﻬﺷﻮﺑ
ﺩﺎﺘﺳﺍ٢
ﻲﻤﻴﺷﻮﻴﺑ ، ﺎﻘﻴﻘﺤﺗ ﺰﮐﺮﻣ ﮏﻳﺰﻴﻓﻮﻴﺑ ﻭ ﻲﻤﻴﺷﻮﻴﺑ ﺕ
، ﻲﻤﻴﺷﻮﻴﺑ ﺶﺨﺑ ﻥﺍﺮﻬﺗ ﻩﺎﮕﺸﻧﺍﺩ ،
ﻩﺪﻴﮑﭼ
ﻪﻨﻴﻣﺯ : ﺕﺪﻣ ﺯﺍ ﻪﺑ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﺶﻴﭘ ﺎﻫ ﺭﺎﻬﻣ ﻪﻠﻤﺟ ﺯﺍ ﻲﺻﺍﻮﺧ ﻥﺁ ﺖﻬﺟ ﻭ ﻩﺩﻮﺑ ﺡﺮﻄﻣ ﻲﺘﻨﺳ ﺐﻃ ﺭﺩ ﻲﻌﻴﺒﻃ ﻱﺍﺰﮔﺭ ﺪﺿ ﻩﺩﺎﻣ ﮏﻳ ﻥﺍﻮﻨﻋ
ﻝﻮﻠﺳ ﺕﺮﺟﺎﻬﻣ ،ﺮﻴﺜﮑﺗ ،ﻲﮔﺪﻨﺒﺴﭼ ﺵﺭﺍﺰﮔ ﻲﻟﻮﻠﺳ ﺝﺭﺎﺧ ﺲﮑﻳﺮﺗﺎﻣ ﻪﻳﺰﺠﺗﻭ ﻢﻀﻫ ﻭ ﻝﺎﻴﻠﺗﻭﺪﻧﺍ ﻱﺎﻫ
ﺖﺳﺍ ﻩﺪﺷ . ﻊﻴﺳﻭ ﻲﻧﺎﻣﺭﺩ ﺩﺮﺑﺭﺎﮐ ﻞﻴﻟﺩ ﻪﺑ ﺍﺬﻟ
،ﻲﻳﺍﺰﮔﺭ ﻝﺮﺘﻨﮐ ﺭﺩ ﻥﺁ ﻑﺪﻫ
ﺮﺿﺎﺣ ﻖﻴﻘﺤﺗ
، ﻦﻴﺌﺗﻭﺮﭘ ﺹﺍﻮﺧ ﻲﺳﺭﺮﺑ ﻲﻣ ﻪﻃﻮﺑﺮﻣ ﻱﺍﺰﮔﺭ ﺪﺿ ﻭ ﻝﺎﻌﻓ ﻱﺎﻫ
ﺪﺷﺎﺑ .
ﺵﻭﺭ ﻭ ﺩﺍﻮﻣ ﺎﻫ
: ﻦﻴﺌﺗﻭﺮﭘ ﺕﺎﺘﺳﺍ ﺮﻓﺎﺑ ﺭﺩ ﻑﻭﺮﻀﻏ ﺖﻓﺎﺑ ﻱﺎﻫ ﻢﻳﺪﺳ
۱۰۰ ﻲﻠﻴﻣ ﺎﺑ ﺭﻻﻮﻣ ۸ / ۴ pH= ﺪﻳﺍﺮﻠﮐﻭﺭﺪﻴﻫ ﻦﻳﺪﻴﻧﺍﻮﮔ ﻱﻭﺎﺣ ۴
ﺭﺩ ﻭ ﺭﻻﻮﻣ
ﺣ ﺎﻬﻣ ﺯﺍ ﻲﻃﻮﻠﺨﻣ ﺭﻮﻀ ﺪﻳﺩﺮﮔ ﺝﺍﺮﺨﺘﺳﺍ ﻲﻤﻳﺰﻧﺁ ﻥﺎﮔﺪﻨﻨﮐﺭ
. ﺯﺍ ﺲﭘ ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ
،ﻥﻮﻴﺗﺎﮐ ﻭ ﻥﻮﻴﻧﺁ ﺾﻳﻮﻌﺗ ﻱﺎﻫ ﻥﻮﺘﺳ ﻱﻭﺭ ﺮﺑ ﻪﻧﻮﻤﻧ ﻦﻴﺌﺗﻭﺮﭘ
ﺎﻫ
ﻲﻳﺍﺰﮔﺭ ﻲﺑﺎﻳﺯﺭﺍ ﺖﻬﺟ ﻭ ﮏﻴﮑﻔﺗ )
ﻪﺧﺎﺷ ﺶﻤﺧ ﺕﺪﺷ ﻭ ﺩﺍﺪﻌﺗ ،ﻕﻭﺮﻋ ﺩﺍﺪﻌﺗ ﻭ ﺖﻣﺎﺨﺿ ﻞﻣﺎﺷ ﺀﺎﺸﻏ ﻲﻌﻴﺒﻃ ﺮﻴﻏ ﮓﻧﺭ ﻭ ﻲﻋﺮﻓ ﻱﺎﻫ
( ﻝﺪﻣ ﺭﺩ
ﻨﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎﺸﻏ ﺪﻧﺪﺷ ﻪﺘﻓﺮﮔ ﺭﺎﮐ ﻪﺑ ﻍﺮﻣ ﻢﺨﺗ ﻦﻴ
.
ﻪﺘﻓﺎﻳ ﺎﻫ : ﺖﻴﻟﺎﻌﻓ ﻦﻳﺮﺘﺸﻴﺑ ﻲﻳﺍﺰﮔﺭ ﺪﺿ
ﻪﻧﻮﻤﻧ ﺭﺩ A1
ﺝﺍﺮﺨﺘﺳﺍ ﻲﻫﺎﻣ ﻪﺳﻮﻛ ﻑﻭﺮﻀﻏ ﺯﺍ ﻩﺪﺷ ﻩﻭﺮﮔ ﻪﺑ ﺖﺒﺴﻧ
ﺪﻫﺎﺷ ﻭ ﻩﻭﺮﮔ ﺮﻳﺎﺳ ﺰﻴﻧ ﺩﺭﻮﻣ ﻱﺎﻫ
ﺶﻳﺎﻣﺯﺁ ﺪﺷ ﻩﺪﻫﺎﺸﻣ .
ﻝﺎﻌﻓ ﻪﻧﻮﻤﻧ ﻲﻨﻴﺌﺗﻭﺮﭘ ﻱﺍﻮﺘﺤﻣ ﻲﺳﺭﺮﺑ A1
ﻱﻭﺭ ﺮﺑ ﺯﺭﻮﻓﻭﺮﺘﮑﻟﺍ ﻝﮊ
، ﻦﻴﺌﺗﻭﺮﭘ ﺩﻮﺟﻭ ﻮﻣ ﻥﺯﻭ ﺎﺑ ﻲﻳﺎﻫ
ﻲﻟﻮﮑﻟ ۱۶ ‐ ۱۴
ﻥﺎﺸﻧ ﺍﺭ ﻥﻮﺘﻟﺍﺩﻮﻠﻴﮐ ﺩﺍﺩ
.
ﻪﺠﻴﺘﻧ ﻱﺮﻴﮔ : ﻦﻴﺌﺗﻭﺮﭘ ﻲﻳﺎﻫ ﺯﺍ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﺱﺭﺎﻓ ﺞﻴﻠﺧ
ﻲﻣ ﻲﻳﺍﺰﮔﺭﺪﺿ ﺕﺍﺮﺛﺍ ﻱﺍﺭﺍﺩ ﺷﺎﺑ
ﻨ ﺪ . ﺭﺩ ﻖﻴﻘﺤﺗ ﺕﺭﻭﺮﺿ ﻭ ﺖﻴﻤﻫﺍ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺍﺬﻟ
ﻱﺭﺎﻤﻴﺑ ﻥﺎﻣﺭﺩ ﻪﻨﻴﻣﺯ ﻦﻴﺌﺗﻭﺮﭘ ﻦﻳﺍ ،ﻲﻳﺍﺰﮔﺭ ﺯﺍ ﻲﺷﺎﻧ ﻱﺎﻫ
ﻭ ﺩﺮﺑﺭﺎﮐ ﻭ ﺮﺘﺸﻴﺑ ﻲﻟﻮﮑﻟﻮﻣ ﺕﺎﻌﻟﺎﻄﻣ ﻡﺎﺠﻧﺍ ﺖﻬﺟ ﺎﻫ ﻱﺭﺎﻤﻴﺑ ﻥﺎﻣﺭﺩ ﺭﺩ ﻲﻳﻭﺭﺍﺩ ﻲﺣﺍﺮﻃ
ﻱﺎﻫ
ﻂﺑﺮﻳﺫ , ﻲﻣ ﺭﺍﺮﻗ ﻪﺟﻮﺗ ﺩﺭﻮﻣ ﺖﺴﻳﺎﺑ ﮔ
ﻴ ﺮ ﻧ ﺪ .
ﻱﺪﻴﻠﮐ ﻥﺎﮔﮊﺍﻭ :
ﻲﻫﺎﻣ ﻪﺳﻮﮐ ،ﻑﻭﺮﻀﻏ ،ﻲﻳﺍﺰﮔﺭ ,
ﻥﺎﻃﺮﺳ
ﻪﻟﺎﻘﻣ ﺖﻓﺎﻳﺭﺩ :
۴ / ۳ / ۱۳۸۶ ‐ ﻪﻟﺎﻘﻣ ﺵﺮﻳﺬﭘ :
۲۳ / ۱۰ / ۱۳۸۶
ﺮﺘﻛﺩ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﯽﻫﺎﮔﺭﺎﺑ
ﺮﻴﺛﺄﺗ
ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﻦﻴﺌﺗﻭﺮﭘ
ﻪﺳﻮﻛ ﻑﻭﺮﻀﻏ ﯽﻫﺎﻣ
/ ۲
ﻪﻣﺪﻘﻣ
ﺎﻳ ﻲﻳﺍﺰﮔﺭ ﺪﻨﻳﺍﺮﻓ ﺖﺧﺎﻨﺷ ﻧﺁ
ﮋ ﻮﻳ ﮊ ﻲﻳﺍﺪﺘﺑﺍ ﺕﺎﻌﻟﺎﻄﻣ ﺎﺑ ﺰﻧ ﮎﺭﻼﮐ
Clark) ( ﺶﻧﺍﺭﺎﮑﻤﻫ ﻭ ﺁ
ﺪﻳﺩﺮﮔ ﺯﺎﻏ )
۱ .(
ﻲﻳﺍﺰﮔﺭ ﺕﺭﻮﺻ ﻪﺑ
ﺪﺷﺭ ﮒﺭ ﺎﻫ ﻪﺧﺎﺷ ﺵﻭﺭ ﻪﺑ ﺪﻳﺪﺟ ﻕﻭﺮﻋ ﻭ ﺸﻨﻣ ﺯﺍ ﻲﻳﺍﺯ
ﺀﺎ
ﮒﺭ ﻱﺎﻫ ﺗ ﻲﻠﺒﻗ ﺃﻮ ﺩﺎﺠﻳﺍ ﺎﺑ ﻡ ﻝﺎﻧﺎﮐ
ﺎﻫ ﻲﻗﻭﺮﻋ ﻦﻴﺑ ﺕﺎﻃﺎﺒﺗﺭﺍ ﻭ
ﻲﻣ ﻒﻳﺮﻌﺗ ﺩﻮﺷ
) ۲ .(
ﺭﺩ ﻲﻳﺍﺰﮔﺭ ﺩﺎﺠﻳﺍ ،ﻲﻌﻗﺍﻮﻣ
ﻪﻠﻤﺟ ﺯﺍ ﺭﺩ
ﻦﻴﺣ ﻭ ﻲﻨﻴﻨﺟ ﻥﺍﺭﻭﺩ ﺭﺩ ﺹﻮﺼﺨﺑ ،ﻮﻤﻧ ﻭ ﺪﺷﺭ ﺎﻳ
ﺦﺳﺎﭘ ﺭﺩ
ﻤﺟ ﺯﺍ ﻲﻌﺿﻮﻣ ﻂﻳﺍﺮﺷ ﻪﺑ ﺘﻓﺎﺑ ﻥﮋﻴﺴﻛﺍ ﺩﻮﺒﻤﻛ ﻪﻠ
ﻲ ﺩﺍﻮﻣ ﻭ
ﻲﻳﺍﺬﻏ ﻲﻣ ﺥﺭ ﻉﻮﻧ ﻲﻟﻭ ،ﺪﻫﺩ ﻭ ﻲﻌﻴﺒﻃ ﺮﻴﻏ
ﻩﺪﺸﻧ ﻝﺮﺘﻨﻛ ﻥﺁ
ﻂﻳﺍﺮﺷ ﺭﺩ ﺐﻠﻏﺍ ﻱﺭﺎﻤﻴﺑ
ﻲﻳﺎﻫ ،ﺎﻣﻮﻳﮋﻧﺁ ﺮﻴﻈﻧ ﺕﺮﺟﺎﻬﻣ
ﺎﻫﺭﻮﻣﻮﺗ
ﻉﺍﻮﻧﺍ ﻭ ﻱﺭﺎﻤﻴﺑ ﻱﺎﻫ ﻭ ﻲﻤﺸﭼ ﻲﺗﺎﭘﻮﻨﻴﺗﺭ ﺮﻳﺎﺳ
ﻲﻳﺎﺳﺭﺎﻧ ﻱﺎﻫ
ﻲﻣ ﻩﺪﻫﺎﺸﻣ ﻲﻗﻭﺮﻋ ﺩﺩﺮﮔ
. ﻭ ﻲﻳﺍﺰﮔﺭ ﺪﻨﻳﺍﺮﻓ ﺖﺧﺎﻨﺷ ﺎﺑ ﺍﺬﻟ
ﻲﻣ ﻥﺁ ﺩﺎﺠﻳﺍ ﻞﻣﺍﻮﻋ ﻲﻌﻴﺳﻭ ﻒﻴﻃ ﻥﺍﻮﺗ
ﻱﺭﺎﻤﻴﺑ ﺯﺍ ﻱﺎﻫ ﻂﺒﺗﺮﻣ
ﻭ ﻝﺮﺘﻨﮐ ﺍﺭ ﻲﻳﺍﺰﮔﺭ ﺎﺑ ﺎﻳ
ﺩﻮﻤﻧ ﻥﺎﻣﺭﺩ )
۳ .(
ﺕﺎﻘﻴﻘﺤﺗ ﻥﻮﻨﮐ ﺎﺗ
ﺭﺩ ﻱﺩﺎﻳﺯ ﺖﻬﺟ ﻭ ﺖﺧﺎﻨﺷ ﺩﺍﻮﻣ ﺪﻴﻟﻮﺗ
ﺪﺿ ﺍﺰﮔﺭ ﻲﻋﻮﻨﺼﻣ ﻲﻳ
ﺝﺍﺮﺨﺘﺳﺍ ﺎﻳ ﻭ ﺩﺍﻮﻣ
ﺪﺿ ﺍﺰﮔﺭ ﻭ ﻲﻌﻴﺒﻃ ﻊﺑﺎﻨﻣ ﺯﺍ ﻲﺣﺍﺮﻃ
-ﺵﻭﺭ
ﻱﺎﻫ ﻩﮋﻳﻭ ﻝﺎﻘﺘﻧﺍ ﺖﻬﺟ ﺩﺍﻮﻣ ﻦﻳﺍ
ﻩﺪﺷ ﻡﺎﺠﻧﺍ ﺖﺳﺍ
) ۴ .(
ﺭﺩ
ﻴﺑﺮﺠﺗ ﻉﻮﻤﺠﻣ ﻕﻮﻓ ﺕﺎ
ﻪﺑ ﺮﺠﻨﻣ ﺮﻛﺬﻟﺍ ﻊﻳﺮﺳ ﺶﻳﺍﺰﻓﺍ
ﺖﺧﺎﻨﺷ
ﻭ ﺩﺍﻮﻣ ﻞﻣﺍﻮﻋ ﻲﻌﻴﺒﻃ ﺭﺎﻬﻣ ﻩﺪﻨﻨﮐ ﻩﺪﻳﺩﺮﮔ ﻲﻳﺍﺰﮔﺭ ﺖﺳﺍ
. ﺯﺍ ﺍﺬﻟ
ﺕﺪﻣ
،ﺶﻴﭘ ﺎﻫ ﺖﻓﺎﺑ ﺑ ﻑﻭﺮﻀﻏ ﻪ
ﻲﻨﻏ ﻲﻌﻴﺒﻃ ﻊﺒﻨﻣ ﻚﻳ ﻥﺍﻮﻨﻋ
ﺡﺮﻄﻣ ﻲﻳﺍﺰﮔﺭ ﻩﺪﻨﻨﮐﺭﺎﻬﻣ ﺩﺍﻮﻣ ﺯﺍ ﺖﺳﺍ ﻩﺩﻮﺑ
) ۵ ( ﻲﻣ ﻪﮐ ﻥﺍﻮﺗ
ﻪﺑ ﻑﻭﺮﻀﻏ ﺮﻴﻈﻧ ﻲﺗﺎﻧﺍﻮﻴﺣ
ﻪﺳﻮﻛ ﻲﻫﺎﻣ ﻪﮐ ﻥﺎﻣﺭﺩ ﺖﻬﺟ
ﺯﺍ ،ﻥﺎﻃﺮﺳ ۳۵
ﻥﻮﻨﻛﺎﺗ ﺶﻴﭘ ﻝﺎﺳ ﻩﺩﻮﺑ ﺡﺮﻄﻣ ﻲﺘﻨﺳ ﺐﻃ ﺭﺩ
-
ﺖﺳﺍ ﺩﺮﮐ ﻩﺭﺎﺷﺍ ، )
۶ .(
ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻣ ﺯﺍ ﻲﮑﻳ
ﺤ ﺩﻭﺪ
ﺖﻓﺎﺑ ﺖﻴﻟﺎﻌﻓ ﺎﺑ ﻲﻳﺍﺰﮔﺭ ﻩﺪﻨﻨﮐﺭﺎﻬﻣ ﺩﺍﻮﻣ ﻱﻭﺎﺣ ﻪﮐ ﺖﺳﺍ ﻲﻳﺎﻫ
ﺖﺳﻻﺎﺑ . ﻪﻳﺮﻈﻧ ﻦﻳﺍ ﻪﺑ ﺮﺠﻨﻣ ﻑﻭﺮﻀﻏ ﺭﺩ ﻲﻧﻮﺧ ﻕﻭﺮﻋ ﻥﺍﺪﻘﻓ
ﻴﻟﻭﺍ ﻪﻛ ﺪﻳﺩﺮﮔ ﻪ ﻝﻮﻠﺳ
ﻱﺎﻫ ﻓﻭﺮﻀﻏ ﻲ ﺪﺿ ﻩﺩﺎﻣ ﺪﻨﭼ ﺎﻳ ﻚﻳ
ﺭﺩ ﺍﺰﮔﺭ ﻳﺮﺗﺎﻣ ﺢﺷﺮﺗ ﺩﻮﺧ ﺲ ﻲﻣ
ﺪﻨﻨﻛ ﻕﻭﺮﻋ ﻞﻴﻜﺸﺗ ﻪﻛ
ﻲﻣ ﺭﺎﻬﻣ ﺍﺭ ﻲﻧﻮﺧ ﺪﻳﺎﻤﻧ
) ۷ .(
ﺍﺬﻟ ﻪﻳﺮﻈﻧ ﻦﻳﺍ ﺱﺎﺳﺍ ﺮﺑ ﺩﺎﻬﻨﺸﻴﭘ
ﻪﮐ ﺪﺷ ﻪﺳﻮﮐ ﻲﻫﺎﻣ ﻧ ﻥﺎﻃﺮﺳ ﺭﺎﭼﺩ ﺎﻫ ﻲﻤ
ﺷ ﻧﻮ ﻥﺎﻃﺮﺳ ﺎﻳ ﻭ ﺪ -
ﺁ ﺭﺩ ﻢﻴﺧﺪﺑ ﻱﺎﻫ ﺎﻬﻧ
ﻲﻣ ﺭﺩﺎﻧ ﺪﺷﺎﺑ . ﺪﺻﺭﺩ ﻦﻳﺮﺘﺸﻴﺑ ﺍﺮﻳﺯ ﺖﺳﺍ ﻲﻓﻭﺮﻀﻏ ﺖﻓﺎﺑ ﺲﻨﺟﺯﺍ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﺖﻠﻜﺳﺍ
) ۱۶
ﺎﻬﻧﺁ ﻥﺪﺑ ﻞﻛ ﻥﺯﻭ ﺯﺍ ﺪﺻﺭﺩ ﺎﺑ ﻪﺴﻳﺎﻘﻣ ﺭﺩ
۶ / ۰ ﺭﺩ ﺪﺻﺭﺩ
ﻪﻟﺎﺳﻮﮔ ( ) ۸ .(
ﻪﺳﻮﻛ ﻑﻭﺮﻀﻏ ﻲﻫﺎﻣ
ﺖﻴﻟﺎﻌﻓ ﻱﺍﺭﺍﺩ ﺎﻫ
ﻳ ﺯﺍ ﻲ
ﺪﺿ ﺖﻴﻟﺎﻌﻓ ﻞﻴﺒﻗ
ﺪﺿ ،ﻲﺑﺎﻬﺘﻟﺍ
ﻪﺒﺷ ﺖﻴﻟﺎﻌﻓ ،ﻲﻳﺍﺰﮔﺭ ﺯﻭﺰﻴﻟ
،ﻲﻣ
ﻢﻳﺰﻧﺁ ﺖﻴﻟﺎﻌﻓ ﺭﺎﻬﻣ ﺯﺎﻧﮊﻼﻛ ﻱﺎﻫ
Iﻉﻮﻧ IVﻭ ، ﺭﺎﻬﻣ ﻭ ﺯﺎﺘﺳﻻﺍ
ﻦﻴﻤﺳﻼﭘ ﻭ ﻦﻴﺴﭙﻳﺮﺗﻮﻤﻴﻛ ،ﻦﻴﺴﭙﻳﺮﺗ ﻞﺜﻣ ﺎﻫﺯﺎﺌﺗﻭﺮﭘ ﺮﻳﺎﺳ ﻲﻣ ﺪﺷﺎﺑ ) ۹ .(
ﺭﻭﺁﺮﻓ ﻩﺩ ﻱﺎﻫ ﻪﻴﻟﻭﺍ ﻥﺎﻣﺭﺩ ﺖﻬﺟ ﻲﻓﻭﺮﻀﻏ
،ﻥﺍﻮﺨﺘﺳﺍ ﻲﻛﻮﭘ ،ﻢﺧﺯ ﻢﻴﻣﺮﺗ ،ﺖﻳﺮﺗﺭﺁ ،ﺲﻳﺯﺎﻳﺭﻮﺴﭘ ،ﻥﺎﻃﺮﺳ ﺭﺍﺮﻗ ﻪﻌﻟﺎﻄﻣ ﺩﺭﻮﻣ ﺖﻴﺗﺎﻣﺭﺩ ﻭ ﺪﻴﺋﻭﺭﻮﻤﻫ ،ﻲﻌﺿﻮﻣ ﺏﺎﻬﺘﻟﺍ ﻪﺘﻓﺮﮔ ﺪﻧﺍ ) ۱۰ .(
ﺩﺮﺑﺭﺎﮐ ﻦﻳﺮﺘﻤﻬﻣ ﻲﻧﺎﻣﺭﺩ
ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ
ﻦﺘﺷﺍﺩ ﻞﻴﻟﺩ ﻪﺑ ﻲﻫﺎﻣ ﺕﺍﺮﺛﺍ
ﻭ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﻲﻧﺎﻃﺮﺳ ﺪﺿ
ﺭﺩ
ﺖﺳﺎﻫﺭﻮﻣﻮﺗ ﻥﺎﻣﺭﺩ )
۱۱ ﻭ ۱۲ .(
،ﻲﻳﻭﺭﺍﺩ ﺵﺯﺭﺍ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﺏﺁ ﺭﺩ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻲﻌﻴﺒﻃ ﺭﻮﻓﻭ ﻭ ﻲﺘﻌﻨﺻ ﻱﺎﻫ
ﻱﺭﻭﺮﺿ ﻪﻨﻴﻣﺯ ﻦﻳﺍ ﺭﺩ ﻱﺮﺘﺸﻴﺑ ﻲﺑﺮﺠﺗ ﺕﺎﻌﻟﺎﻄﻣ ،ﻥﺍﺮﻳﺍ ﻡﺮﮔ ﻲﻣ ﺪﺷﺎﺑ
؛ ﻱﺯﺎﺳﺍﺪﺟ ﻭ ﺝﺍﺮﺨﺘﺳﺍ ﻖﻴﻘﺤﺗ ﻦﻳﺍ ﺯﺍ ﻑﺪﻫ ﺍﺬﻟ
ﻦﻴﺌﺗﻭﺮﭘ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺮﺛﺍ ﻲﺑﺎﻳﺯﺭﺍ ﻭ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻱﺎﻫ
ﻱﺪﻴﺘﭙﭘ ﻱﻭﺭﺍﺩ ﮏﻳ ﻲﺣﺍﺮﻃ ﺭﻮﻈﻨﻣ ﻪﺑ ﺎﻬﻧﺁ ﻥﺎﻣﺭﺩ ﺖﻬﺟ
ﻱﺭﺎﻤﻴﺑ ﺖﺳﺍ ﻲﻳﺍﺰﮔﺭ ﺎﺑ ﻂﺒﺗﺮﻣ ﻱﺎﻫ .
ﺵﻭﺭ ﻭ ﺩﺍﻮﻣ ﺭﺎﮐ
ﻉﻮﻧ ﻥﮊﻼﮐ ﺝﺍﺮﺨﺘﺳﺍ I
ﻥﻭﺪـــﻧﺎﺗ ﺯﺍ ﺵﻮـــﻣ ﻡﺩ ﻱﺎـــﻫ
ﻩﺩﺎـــﻣ ﻲﻳﺍﺮﺤـــﺻ ﻱﺎـــﻫ
Sprague-Dawely ﻦﺳ ﻪﺑ
۱۲ ‐ ۸
ﺪـﻳﺩﺮﮔ ﻩﺩﺎﻔﺘﺳﺍ ﻪﺘﻔﻫ .
ﺭﺩ ﻥﻭﺪـﻧﺎﺗ ﻡﺮـﮔ ﮏـﻳ ،ﺝﺍﺮﺨﺘـﺳﺍ ﺖﻬﺟ ۳۰۰
ﻲـﻠﻴﻣ ﺮـﺘﻴﻟ
ﺪﻴﺳﺍ ﻝﻮﻠﺤﻣ ﮏﻴﺘﺳﺍ
۰۰۱ / ۰
% ـﺑ ﺕﺪـﻣ ﻪ ۴۸ ﺭﺩ ﺖﻋﺎـﺳ
ﺎﻣﺩ ﻱ ۴ ﻲﺘﻧﺎﺳ ﻪﺟﺭﺩ ﺲـﭘ ﻭ ﺪـﺷ ﻩﺩﺯ ﻢـﻬﺑ ﻲﻣﺍﺭﺁ ﻪﺑ ﺩﺍﺮﮔ
ﺪﻳﺩﺮﮔ ﻩﺩﺎﻔﺘﺳﺍ ﻥﺁ ﻲﻳﻭﺭ ﻝﻮﻠﺤﻣ ﮊﻮﻔﻳﺮﺘﻧﺎﺳﺯﺍ
) ۱۳ .(
ﻑﻭﺮﻀﻏ ﺖﻓﺎﺑ ﺯﺍ ﻦﻴﺌﺗﻭﺮﭘ ﺝﺍﺮﺨﺘﺳﺍ
ﻪﺳﻮﮐ ﻲﻫﺎﻣ ﻉﻮﻧ ﻱﺎﻫ Carcharhinus sorrah ﻲﺒﻳﺮﻘﺗ ﻥﺯﻭ ﺎﺑ
ﺱﺭﺎــﻓ ﺞﻴــﻠﺧ ﺕﻼﻴــﺷ ﺯﺍ ﻡﺮﮔﻮــﻠﻴﮐ ﻭﺩ )
ﺏﺁ ﻞﺣﺍﻮــﺳ ﻱﺎــﻫ
۳ / ﺏﻮﻨﺟ ﺐﻃ
ﻝﺎﺳ
ﻢﻫﺩ ﻩﺭﺎﻤﺷ ۱ / ﺭﻮﻳﺮﻬﺷ ۱۳۸۶
ﺮﻬﺷﻮﺑ ( ﺎﻣﺯﺁ ﻪﺑ ﺪﻤﺠﻨﻣ ﺕﺭﻮﺻ ﻪﺑ ﻭ ﺪﺷ ﻪﻴﻬﺗ ﻞـﻤﺣ ﻩﺎﮕـﺸﻳ
ﺪﻳﺩﺮﮔ . ﺶﺨﺑ ،ﻦﻳﺯﻮـﺗ ﺕﺍﺮﻘﻓ ﻥﻮﺘﺳ ﻭ ﻡﺩ ،ﻪﻟﺎﺑ ﻲﻓﻭﺮﻀﻏ ﻱﺎﻫ
ﻪﺑ ﻲﻗﺮﺑ ﻦﮐﺩﺮﺧ ﻩﺎﮕﺘﺳﺩ ﻂﺳﻮﺗ ﺲﭙﺳ ﻭ ﻲﻧﻮﻔﻋﺪﺿ ،ﻮﺸﺘﺴﺷ ﺪﺷ ﻞﻳﺪﺒﺗ ﺭﺩﻮﭘ .
ﺕﺎﺘـﺳﺍ ﺮﻓﺎـﺑ ﺯﺍ ﻦﻴﺌﺗﻭﺮـﭘ ﺝﺍﺮﺨﺘﺳﺍ ﺖﻬﺟ -
ﻢﻳﺪــﺳ ۱۰۰ ﻲــﻠﻴﻣ ﺎــﺑ ﺭﻻﻮــﻣ ۸
/ ۴ pH ‐ ﻦﻳﺪــﻴﻧﺍﻮﮔ ﻱﻭﺎــﺣ
ﺪﻳﺍﺮﻠﮐﻭﺭﺪﻴﻫ ۴
ﻥﺎﮔﺪـﻨﻨﮐﺭﺎﻬﻣ ﺯﺍ ﻲﻃﻮﻠﺨﻣ ﺭﻮﻀﺣ ﺭﺩ ﻭ ﺭﻻﻮﻣ
ﻧﺁ ﺪﻳﺩﺮﮔ ﻩﺩﺎﻔﺘﺳﺍ ﻲﻤﻳﺰ .
ﺕﺪـﻣ ﻪﺑ ﺝﺍﺮﺨﺘﺳﺍ ﻞﻤﻋ ۴۸
ﺖﻋﺎـﺳ
ﻱﺎﻣﺩ ﺭﺩ ۴ ﻲﺘﻧﺎـﺳ ﻪﺟﺭﺩ ﺪـﺷ ﻡﺎـﺠﻧﺍ ﺩﺍﺮـﮔ
) ۱۴ .(
ﻱﺎـﻧﮊﻮﻤﻫ
ﺭﺩ ﻞﺻﺎﺣ
×g ۱۵۰۰۰۰ ﻱﺎـﻣﺩ ﺭﺩ ﺖﻋﺎـﺳ ﻢﻴـﻧ ﺕﺪـﻣ ﻪـﺑ ۴
ﻲﺘﻧﺎﺳ ﻪﺟﺭﺩ ﺖـﻬﺟﻭ ﻆﻴﻠﻐﺗ ﻲﻳﻭﺭ ﻊﻳﺎﻣ ﻭ ﺪﺷ ﮊﻮﻔﻳﺮﺘﻧﺎﺳ ﺩﺍﺮﮔ
ﺪﻳﺩﺮﮔ ﻱﺭﺍﺪﻬﮕﻧ ﻱﺪﻌﺑ ﻩﺩﺎﻔﺘﺳﺍ .
ﻲﻧﻮﻴﻧﺁ ﺾﻳﻮﻌﺗ ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ
ﺯﺎﺳﺍﺪﺟ ﺖﻬﺟ ﻦﻴﺌﺗﻭﺮـﭘ ﻱ
ﻱﺎـﻧﮊﻮﻤﻫ ﺭﺩ ﺩﻮـﺟﻮﻣ ﻱﺎـﻫ
ﻲﻓﺍﺮﮔﻮﺗﺎـﻣﻭﺮﮐ ﻥﻮﺘـﺳ ﺯﺍ ﻲﻫﺎـﻣ ﻪـﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻪﻴﻟﻭﺍ ﻥﻮﻴﻧﺁ ﺾﻳﻮﻌﺗ Q-Sepharose FF
ﺩﺎـﻌﺑﺍ ﻪﺑ cm)
۲ / ۵
× ۱۴ ( ﺮﻓﺎﺑ ﻂﻳﺍﺮﺷ ﺭﺩ Tris–HCl
ﺖﻈﻠﻏ ﻪﺑ ۵۰
ﻲـﻠﻴﻣ
،ﺭﻻﻮﻣ ۱ / ۸ pH‐ ﻩﺭﻭﺍ ﻱﺍﺭﺍﺩ ۶
ﺪـﻳﺩﺮﮔ ﻩﺩﺎﻔﺘﺳﺍ ﺭﻻﻮﻣ .
ﮐﺬﻣ ﺮﻓﺎﺑ ﻪﻴﻠﻋ ﺰﻴﻟﺎﻳﺩ ﺯﺍ ﺲﭘ ﻲﻨﻴﺌﺗﻭﺮﭘ ﻪﻧﻮﻤﻧ ﻥﻮﺘﺳ ﺮﺑ ﺭﻮ
ﮏـﻳ ﻥﺎـﻳﺮﺟ ﺖﻋﺮـﺳ ﺎـﺑ ﻥﻮﺘﺳ ﺲﭙﺳ ﻭ ﺪﺷ ﻩﺩﺍﺩ ﺭﺍﺮﻗ ﻲﻠﻴﻣ ﺪﺷ ﻩﺩﺍﺩ ﻮﺸﺘﺴﺷ ﻪﻘﻴﻗﺩ ﺮﺑ ﺮﺘﻴﻟ .
ﮏﻴﭘ ﺝﻭﺮﺧ ﺯﺍ ﺲﭘ
ﺖـﻈﻠﻏ ﺯﺍ ﻡﻭﺩ ﮏـﻴﭘ ﮏﻴﮑﻔﺗ ﺖﻬﺟ ،ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻝﻭﺍ NaCl ﮏﻤﻧ ﺪﻳﺩﺮﮔ ﻩﺩﺎﻔﺘﺳﺍ ﺭﻻﻮﻣ ﮏﻳ .
ﺏﺬـﺟ ﻥﺍﺰـﻴﻣ
ﻪﻧﻮﻤﻧ ﺝﻮﻣ ﻝﻮﻃ ﺭﺩ ﺎﻫ ۲۲۵
ﻩﺯﺍﺪﻧﺍ ﺮﺘﻣﻮﻧﺎﻧ ﻭ ﺪـﺷ ﻱﺮـﻴﮔ
ﻪــﻧﻮﻤﻧ ﻢــﺠﺣ ﻪــﺑ ﻲﻳﺎــﻫ ﻲــﻠﻴﻣ ﻭﺩ
ﻥﻮﺘــﺳ ﺮــﻳﺯ ﺯﺍ ﺮــﺘﻴﻟ
ﻊﻤﺟ ﺪﻳﺩﺮﮔ ﻢﺳﺭ ﻪﻃﻮﺑﺮﻣ ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻲﻨﺤﻨﻣ ﻭ ﻱﺭﻭﺁ .
ﻲﻧﻮﻴﺗﺎﮐ ﺾﻳﻮﻌﺗ ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ
ﻥﻮﺘـﺳ ﺯﺍ ﻲﻧﻮﻴﺗﺎـﮐ ﺾﻳﻮـﻌﺗ ﻲﻓﺍﺮﮔﻮﺗﺎـﻣﻭﺮﮐ ﻡﺎﺠﻧﺍ ﺖﻬﺟ ﻥﻮﻴﺗﺎـــﮐ ﺾﻳﻮـــﻌﺗ Sp-Sepharose FF
ﺩﺎـــﻌﺑﺍ ﻪـــﺑ
cm) ۸ /
×۱ ۱۵ ( ﻢﻴﺳﺎﺘﭘ ﺕﺎﻔﺴﻓ ﺮﻓﺎﺑ ﻂﻳﺍﺮﺷ ﺭﺩ ۵۰
ﻲـﻠﻴﻣ -
ﻥﺍﺰـﻴﻣ ﻪﺑ ﺭﻻﻮﻣ ۸
/ ۵ pH ‐ ﻭﺎـﺣ ﻩﺭﻭﺍ ﻱ ۵ ﻩﺩﺎﻔﺘـﺳﺍ ﺭﻻﻮـﻣ
ﺪﻳﺩﺮﮔ . ﻪﻧﻮﻤﻧ ،ﻕﻮـﻓ ﺮﻓﺎﺑ ﻪﻴﻠﻋ ﺰﻴﻟﺎﻳﺩ ﺯﺍ ﺲﭘ ﻲﻨﻴﺌﺗﻭﺮﭘ ﻱﺎﻫ ﻂـﺳﻮﺗ ﻥﻮﺘﺳ ﻱﻮﺸﺘﺴﺷ ﺯﺍ ﺲﭘ ﻭ ﺪﺷ ﻩﺩﺍﺩ ﺭﺍﺮﻗ ﻥﻮﺘﺳ ﺮﺑ
ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻝﻭﺍ ﮏﻴﭘ ﺝﻭﺮﺧ ﻭ ﺭﻮﮐﺬﻣ ﺮﻓﺎﺑ )
ﮏـﻴﭘ A0
( ،
ﻦﻴﺌﺗﻭﺮﭘ ﺮﻳﺎﺳ ﺝﻭﺮﺧ ﺖﻬﺟ ﺯﺍ ،ﻥﻮﺘـﺳ ﻪﺑ ﻩﺪﺷ ﺏﺬﺟ ﻱﺎﻫ
ﻢﻴﺳﺎﺘﭘ ﺕﺎﻔﺴﻓ ﺮﻓﺎﺑ ۵۰
ﻲﻠﻴﻣ ﻩﺭﻭﺍ ،ﺭﻻﻮـﻣ ۵
،ﺭﻻﻮـﻣ ۸ / ۵ ‐
ﮏــﻤﻧ ﻪﺘــﺳﻮﻴﭘﺎﻧ ﻥﺎــﻳﺩﺍﺮﮔ ﻱﻭﺎــﺣpH ۵۰
، ۱۰۰ ، ۲۰۰ ﻭ
۴۰۰ ﻲــﻠﻴﻣ ﺭﻻﻮــﻣ ﻡﺍﺮﮔﻮﺗﺎــﻣﻭﺮﮐ ﻭ ﺪــﺷ ﻩﺩﺎﻔﺘــﺳﺍNaCl
ﻩﺯﺍﺪﻧﺍ ﺎﺑ ﻪﻃﻮﺑﺮﻣ ﺝﻮـﻣ ﻝﻮـﻃ ﺭﺩ ﺏﺬـﺟ ﻥﺍﺰﻴﻣ ﻱﺮﻴﮔ
۲۲۵
ﺪﻳﺩﺮﮔ ﻢﺳﺭ ﺮﺘﻣﻮﻧﺎﻧ .
ﻦﻴﺌﺗﻭﺮﭘ ﺶﺠﻨﺳ
ﺭﺎﮐ ﻪﺑ ﻱﺭﻮﻟ ﻩﺪﺷ ﺡﻼﺻﺍ ﺵﻭﺭ ﻦﻴﺌﺗﻭﺮﭘ ﺖﻈﻠﻏ ﻦﻴﻴﻌﺗ ﺖﻬﺟ ﺪﺷ ﻪﺘﻓﺮﮔ . ﺌﺗﻭﺮﭘ ﻝﻮﻠﺤﻣ ﺯﺍ ﻱﻭﺎـﮔ ﻡﺮـﺳ ﻦﻴﻣﻮﺒﻟﺁ ﻦﻴ
BSA) (
ﻲﻨﻴﺌﺗﻭﺮﭘ ﺩﺭﺍﺪﻧﺎﺘﺳﺍ ﻝﻮﻠﺤﻣ ﻪﻴﻬﺗ ﺖﻬﺟ ﺪﻳﺩﺮﮔ ﻩﺩﺎﻔﺘﺳﺍ
) ۱۵ .(
ﺪﻴﻣﺁ ﻞﻳﺮﻛﺁ ﻲﻠﭘ ﻝﮊ ﺯﺭﻮﻓﻭﺮﺘﻜﻟﺍ SDS–PAGE)
(
ﻝﮊ ﻱﻭﺭ ﺮﺑ ﻲﻠﻣﻻ ﺵﻭﺭ ﺱﺎﺳﺍﺮﺑ ﺯﺭﻮﻓﻭﺮﺘﮑﻟﺍ ۱۲
ﺪﺻﺭﺩ
ﺪﻴﻣﺁ ﻞﻳﺮﮐﺍ ﻲﻠﭘ ﻪﺘﺳﻮﻴﭘﺎﻧ SDS ‐
ﮊﺎﺘﻟﻭ ﺭﺩ ۱۰۰ ﺖـﻟﻭ ﻭ
ﺪﻳﺩﺮﮔ ﻡﺎﺠﻧﺍ ﺖﺑﺎﺛ ﻥﺎﻳﺮﺟ ﺕﺪﺷ )
۱۶ .(
ﻝﮊ ﺲﭙﺳ ﻂـﺳﻮﺗ
ﻲﺳﺎﻣﻮﻛ ﮓﻧﺭ ﻝﻮﻠﺤﻣ R-250
ﮓﻧﺭ ﺪﺷ ﻱﺰﻴﻣﺁ .
ﻪﺟﻮﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎﺸﻏ ﻂﺳﻮﺗ ﻲﻳﺍﺰﮔﺭ ﺶﺠﻨﺳ
ﻍﺮﻣ ﻢﺨﺗ ﺍﺪﺘﺑﺍ ﺶﻳﺎﻣﺯﺁ ﻦﻳﺍ ﺭﺩ ﺩﺍﮋﻧ ﺯﺍ ﻲﻳﺎﻫ
ﻥﺎﻣﻮﻟ Lohman) (
ﺪـﻧﺪﻳﺩﺮﮔ ﻲﻫﺩﺯﺎـﮔ ﻭ ﻲﻧﻮﻔﻋﺪﺿ ﻦﻴﻟﺎﻣﺮﻓ ﺯﺎﮔ ﻂﺳﻮﺗ .
ﺖـﻬﺟ
ﻩﻭﺮﮔ ﺰﻴﻧ ﻭ ﻲﺸﻳﺎﻣﺯﺁ ﻩﻭﺮﮔ ﺮﻫ ﻢـﺨﺗ ﺩﺪـﻋ ﺞﻨﭘ ﺩﺍﺪﻌﺗ ،ﺪﻫﺎﺷ
ﻓﺮﮔ ﺭﺎﮐ ﻪﺑ ﻍﺮﻣ ﻪﺟﻮﺟ ﺭﻮﺗﺎﺑﻮﮑﻧﺍ ﺭﺩ ﺲﭙﺳ ﻭ ﺪﺷ ﻪﺘ
ﺭﺩ ﻲﺸﮐ
ﻲﻳﺎﻣﺩ ﻂﻳﺍﺮﺷ ۳۷
ﻲﺘﻧﺎـﺳ ﻪـﺟﺭﺩ ﺖـﺑﻮﻃﺭ ﻭ ﺩﺍﺮـﮔ
۶۰ ‐ ۵۵
ﺪﻧﺪﺷ ﻩﺩﺍﺩ ﺭﺍﺮﻗ ﺪﺻﺭﺩ .
ﻲﻳﺍﻮـﻫ ﻪﺘـﺳﻮﭘ ﻪﻴﺣﺎﻧ ﻢﺘﺸﻫ ﺯﻭﺭ ﺭﺩ
ﻍﺮــﻣ ﻢــﺨﺗ ﺀﺎــﺸﻏ ﻲﻧﺎــﻗﻮﻓ ﺢﻄــﺳ ﺎــﺗ ﺪــﺷ ﻩﺩﺍﺩ ﺵﺮــﺑ ﺎــﻫ
ﺩﺩﺮﮔ ﻥﺎﻳﺎﻤﻧ ﻦﻴﻨﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ .
ﺰﻟﻮﻠﺳ ﻞﻴﺘﻣ ﻝﮊ ﻁﻮﻠﺨﻣ
ﻪﻧﻮﻤﻧ ﻱﻭﺎﺣ ﺩ ﻪﺑ ﻦﻴﺌﺗﻭﺮﭘ ﻱﺎﻫ
ﮏـﻳ ﻁﻼﺘـﺧﺍ ﺯﺍ ،ﻩﺪـﻣﺁ ﺖﺳ
ﻝﮊ ﻢﺠﺣ ۱
% ﺰﻟﻮﻠﺳ ﻞﻴﺘﻣ )
ﻪﺘﻳﺯﻮﮑﺴﻳﻭ ۴۰۰۰
ﻲﺘﻧﺎـﺳ ﺯﺍﻮـﭘ (
ﺎﺑ ﻲﻨﻴﺌﺗﻭﺮﭘ ﻪﻧﻮﻤﻧ ﻝﻮﻠﺤﻣ ﺯﺍ ﻢﺠﺣ ﮏﻳ ﻁﻮـﻠﺨﻣ ﻱﺍ ﻪـﻧﻮﮕﺑ
ﻪﻛ ﺪﺷ ﻲﻳﺎﻬﻧ ﻪﺘﻳﺯﻮﮑﺴﻳﻭ ﺎﺑ ۵
/ ۰ ﻲﻳﺎـﻬﻧ ﺖـﻈﻠﻏ ﻭ ﺪﺻﺭﺩ ۲
ﻦﻴﺌﺗﻭﺮﭘ ﺮﺘﻴﻟﻭﺮﮑﻴﻣ ﺮﺑ ﻡﺮﮔﻭﺮﮑﻴﻣ ﺩﺩﺮـﮔ ﻞﺻﺎﺣ
) ۱۷ .(
ﻩﺎـﮕﻧﺁ
ﺮﺘﻛﺩ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﯽﻫﺎﮔﺭﺎﺑ
ﺮﻴﺛﺄﺗ
ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﻦﻴﺌﺗﻭﺮﭘ
ﻪﺳﻮﻛ ﻑﻭﺮﻀﻏ ﯽﻫﺎﻣ
/ ۴
ﮎﻼﭘ ﻪﻴﻬﺗ ﺖﻬﺟ ﻞﻴﺘﻣ ﻱﺎﻫ
ﺍﺯﺍ ﻪﺑ ﺰﻟﻮﻠﺳ ﺭﺍﺪﻘﻣ ﮎﻼﭘ ﺮﻫ ﺀ
۵۰
ﻦﻴﺌﺗﻭﺮﭘ ﻁﻮﻠﺨﻣ ﺯﺍ ﺮﺘﻴﻟﻭﺮﮑﻴﻣ ‐
ﺭﺍﻮـﻧ ﺢﻄـﺳ ﺮـﺑ ﺭﻮﮐﺬﻣ ﻝﮊ
ﺖﻋﺎـﺳ ﻢﻴـﻧ ﺕﺪـﻣ ﻪـﺑ ﺩﻮﻫ ﺮﻳﺯ ﺭﺩ ﻭ ﺪﺷ ﻩﺩﺍﺩ ﺭﺍﺮﻗ ﻥﻮﻠﻔﺗ ﺪﻳﺩﺮﮔ ﮏﺸﺧ .
ﮎﻼﭘ ﺭﻮﻈﻨﻣ ﻦﻳﺪﺑ ﻱﻭﺎـﺣ ﺰﻟﻮﻠـﺳ ﻞﻴﺘﻣ ﻱﺎﻫ
ﺖﻈﻠﻏ ۱۰۰ ﻡﺮﮔﻭﺮﮑﻴﻣ ﺯﺍ ﮏﻳ ﺮﻫ ﻪﺑ ﺖﺒﺴﻧ ﻦﻴﺌﺗﻭﺮﭘ
ﻪـﻧﻮﻤﻧ -
A ﻱﺎﻫ B ﻭ ﻦﻴﻨﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎﺸﻏ ﺮﺑ ﻭ ﻪﻴﻬﺗ ﺭﺩ ﺎـﻫ
ﻩﻭﺮﮔ ﺪﺷ ﻩﺩﺍﺩﺭﺍﺮﻗ ﻲﺸﻳﺎﻣﺯﺁ ﻱﺎﻫ .
ﻩﻭﺮﮔ ﺪﻫﺎﺷ ﻂـﻘﻓ ﮎﻼـﭘ -
ﻱﻭﺎﺣ ﺰﻟﻮﻠﺳ ﻞﻴﺘﻣ ﻱﺎﻫ ﺮﻓﺎﺑ
ﻢﻳﺪﺳ ﺕﺎﻔﺴﻓ ۵۰
ﻲﻠﻴﻣ ﺭﻻﻮـﻣ
۴ / ۷ pH= ﺩﻮﻤﻧ ﺖﻓﺎﻳﺭﺩ .
ﻢﺨﺗ ﻍﺮﻣ ﻪـﺑ ﻢـﻫﺩ ﺯﻭﺭ ﺎﺗ ﹰﺍﺩﺪﺠﻣ ﺎﻫ
ﺪﻧﺪﺷ ﻩﺪﻧﺍﺩﺮﮔﺮﺑ ﺭﻮﺗﺎﺑﻮﮑﻧﺁ .
ﮏـﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎـﺸﻏ ﺲﭙـﺳ
ﻞﻣﺎﺷ ﻩﺪﻨﻨﮐ ﺖﻴﺒﺜﺗ ﻝﻮﻠﺤﻣ ﻂﺳﻮﺗ ۳
ﺪﻴﺋﺪﻟﺁﺭﺎﺗﻮﻠﮔ ﺪﺻﺭﺩ ﺭﺩ
ﺕﻼﻳﺩﻮﮐﺎﮐ ﺮﻓﺎﺑ ۱
/ ۰ ﺎـﺑ ﺭﻻﻮـﻣ ۴
/ ۷ pH= ﻭ ﻩﺪـﻳﺩﺮﮔ ﺖـﻴﺒﺜﺗ
ﺯﺍ ﻦﻴــﺑﺭﻭﺩ ﻪــﺑ ﺰــﻬﺠﻣ ﭖﻮﮑــﺳﻭﺮﮑﻴﻣﻮﺋﺮﺘﺳﺍ ﻂــﺳﻮﺗ ﻩﺎـﮕﻧﺁ ﺪﺷ ﻱﺭﺍﺩﺮﺑ ﺮﻳﻮﺼﺗ ﻪﻧﻮﻤﻧ ﺮﻴﺛﺄﺗ ﺖﺤﺗ ﻲﺣﺍﻮﻧ .
ﻲﻳﺍﺰﮔﺭ ﻥﺍﺰﻴﻣ
ﻝﻮﻠﺳ ﺵﺮﺘﺴﮔ ﻭ ﺵﻭﺭ ﻪـﺑ ﺪﻫﺎـﺷ ﻩﻭﺮـﮔ ﺎـﺑ ﻪﺴﻳﺎﻘﻣ ﺭﺩ ﺎﻫ
ﻩﺪﻫﺎـﺸﻣ ﻱﺍ Blinded observer method) (
ﺮـﻈﻧ ﺭﺩ ﺎـﺑ
ﻲﻳﺎﻫﺭﺎﻴﻌﻣ ﻦﺘﻓﺮﮔ ،ﺢﻄـﺳ ﺪـﺣﺍﻭ ﺭﺩ ﻕﻭﺮـﻋ ﺩﺍﺪﻌﺗ ﻪﻠﻤﺟ ﺯﺍ
ﺶﻤﺧ ﺕﺪﺷ ﻪﺧﺎﺷ ﺩﺍﺪﻌﺗ ،ﺎﻫ
ﺮﻄﻗ ،ﻲﻌﻴﺒﻃ ﮓﻧﺭ ،ﻲﻋﺮﻓ ﻱﺎﻫ
ﻲﻔﻴﮐ ﻭ ﻲﻤﮐ ﻱﺎﻫﺭﻮﺘﮐﺎﻓ ﺮﻳﺎﺳ ﻭ ﻕﻭﺮﻋ ﺮـﻳﺯ ﺡﺮﺸﺑ
ﻪـﺟﺭﺩ
ﺪﻳﺩﺮﮔ ﻱﺪﻨﺑ )
۱۸ .(
ﺪﺷ ﻡﺎﺠﻧﺍ ﻲﻔﻴﻛ ﺕﺭﻮﺼﺑ ﻲﻳﺍﺰﮔﺭ ﺭﺎﻴﻌﻣ :
ﻲـﻳﺍﺰﮔﺭ ﺭﺎﻬﻣ ﺮﺜﮐﺍﺪﺣ
(+++) ﻥﺍﺰـﻴﻣ ﺯﺍ ﺮﺘﻤﮐ ﻲﻟﻭ ﻪﻈﺣﻼﻣ ﻞﺑﺎﻗ ﻲﻳﺍﺰﮔﺭ ﺭﺎﻬﻣ ، ﻕﻮـﻓ
(++)
، ﻲﻳﺍﺰﮔﺭ ﺭﺎﻬﻣ ﻞﻗﺍﺪﺣ (+)
ﻭ ﺗ ﻥﻭﺪﺑ ﺄ ﻲﻳﺍﺰﮔﺭ ﺮﺑ ﺮﻴﺛ )
± (.
ـﻣ ﻱﺭﺎـﻣﺁ ﺵﻭﺭ ﻪـﺑ ﻩﺪـﻣﺁ ﺖﺳﺪﺑ ﺞﻳﺎﺘﻧ ﺰﻴﻟﺎﻧﺁ ﺖﻬﺟ ﺎ
ﻥ
ﻲﻨﺘﻳﻭ ﻮﻳ
، ﻩﻭﺮﮔ ﺎﺑ ﺶﻳﺎﻣﺯﺁ ﺩﺭﻮﻣ ﻩﻭﺮﮔ ﺮﻫ ﺪﻫﺎـﺷ
ﺎـﺑ ﻭ
ﻦﺘﻓﺮﮔ ﺮﻈﻧ ﺭﺩ )
۰۵ / P <۰ ( ﺪﻳﺩﺮﮔ ﻪﺴﻳﺎﻘﻣ .
ﻪﺘﻓﺎﻳ ﺎﻫ
ﻞﮑﺷ ) ۱ ( ﻑﻭﺮﻀﻏ ﻩﺭﺎﺼﻋ ﺯﺍ ﻞﺻﺎﺣ ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ
ﻪﺳﻮﮐ ﻲﻣ ﻥﺎﺸﻧ ﺍﺭ ﻲﻫﺎﻣ ﺪﻫﺩ
.
ﻞﮑﺷ ۱ : ﺯﺍ ﻞﺻﺎﺣ ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻩﺭﺎﺼﻋ
.
(A ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ Q- Sepharose
ﺗ ﺮﻓﺎﺑ ﺭﺩ ﻪﻴﻟﻭﺍ ﻪﻧﻮﻤﻧ ﺲﻳﺮ
۵۰ ﻲﻠﻴﻣ
،ﺭﻻﻮﻣ ﻩﺭﻭﺍ ۶ ﺭﻻﻮﻣ ۸ pH= . ﻪﺘﺳﻮﻴﭘ ﻥﺎﻳﺩﺍﺮﮔ )NaCl
...
. ( ؛ ﺭﺩ ﻪﻧﻮﻤﻧ ﺏﺬﺟ ۲۲۵
ﺮﺘﻣﻮﻧﺎﻧ ) ‐
◊
‐ ( .
(B ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ Sp-Sepharose
ﻪﻧﻮﻤﻧ ﺭﺩA ﺮﻓﺎﺑ ﺕﺎﻔﺴﻓ ﻢﻴﺳﺎﺘﭘ ۵۰ ﻲﻠﻴﻣ
،ﺭﻻﻮﻣ ﻩﺭﻭﺍ ۵ ،ﺭﻻﻮﻣ ۴ / ۵ pH= ﻂﻳﺍﺮﺷ ﺭﺩ ﻥﺎﻳﺩﺍﺮﮔ ﻪﻠﺣﺮﻣ ﻱﺍ ۴۰۰ , ۲۰۰ , ۱۰۰ ﻭ ۵۰
ﻲﻠﻴﻣ NaClﺭﻻﻮﻣ ) . ...
(
؛ ﺏﺬﺟ ﺭﺩ ﻪﻧﻮﻤﻧ ۲۲۵ ﺮﺘﻣﻮﻧﺎﻧ )
◊‐
‐ ( .
۵ / ﺏﻮﻨﺟ ﺐﻃ
ﻝﺎﺳ
ﻢﻫﺩ ﻩﺭﺎﻤﺷ ۱ / ﺭﻮﻳﺮﻬﺷ ۱۳۸۶
ﻱﺭﻮﻃ ﻪﺑ ﻞﮑﺷ ﺭﺩ ﻪﮐ
A1) ( ﻲﻣ ﻩﺪﻫﺎﺸﻣ ﻪﻧﻮﻤﻧ ﺩﻮﺷ
ﮏﻴﭘ ﺮﺑ ﻖﺒﻄﻨﻣ ﻪﻧﻮﻤﻧ ﻭﺩ ﻪﺑ ﻪﻴﻟﻭﺍ ﻭA
ﻩﺪﺷ ﮏﻴﮑﻔﺗ B
ﺖﺳﺍ . ﺖﻬﺟ ﻲﺑﺎﻳﺯﺭﺍ ﺗ ﺮﻴﺛﺄ ﻪﻧﻮﻤﻧ ﻲﻳﺍﺰﮔﺭﺪﺿ ﺎﻫ
،
ﮏﻴﭘ ﻱﺎﻫ ﻭ A ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻥﻮﺘﺳ ﺯﺍ B ﻞﮑﺷ
A1) (
ﻊﻤﺟ ﻱﺭﻭﺁ ﺖﻬﺟ ﻭ ﻲﻳﺍﺰﮔﺭ ﺶﺠﻨﺳ
ﺮﺑ ﺀﺎﺸﻏ
ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﻪﺟﻮﺟ
ﺪﺷ ﻪﺘﻓﺮﮔ ﺭﺎﮑﺑ .
ﻪﮐ ﺭﻮﻃ ﻥﺎﻤﻫ
ﺭﺩ ﻞﮑﺷ ) ۲ ( ﻲﻣ ﻩﺪﻫﺎﺸﻣ ﺩﻮﺷ
ﻓﺎﻳﺭﺩ ﻩﻭﺮﮔ ﻩﺪﻨﻨﮐ ﺖ
ﻪﻧﻮﻤﻧ ﺮﺘﻣﺍﺭﺎﭘ ﺱﺎﺳﺍ ﺮﺑA
ﻪﻠﻤﺟﺯﺍ ﻲﻳﺍﺰﮔﺭ ﻲﺑﺎﻳﺯﺭﺍ ﻱﺎﻫ
ﺩﺍﺪﻌﺗ ﻭ ﺖﻣﺎﺨﺿ ﺶﻤﺧ ﺕﺪﺷ ﻭ ﺩﺍﺪﻌﺗ ،ﻕﻭﺮﻋ
ﻪﺧﺎﺷ
،ﺀﺎﺸﻏ ﻲﻌﻴﺒﻃﺮﻴﻏ ﮓﻧﺭ ﻭ ﻲﻋﺮﻓ ﻱﺎﻫ ﻦﻳﺮﺘﺸﻴﺑ
ﻲﻳﺍﺰﮔﺭ ﺭﺎﻬﻣ ﺖﻴﻟﺎﻌﻓ ﻦﻴﻨﭽﻤﻫ ،ﺪﻫﺎﺷ ﻩﻭﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ﺍﺭ
ﻩﻭﺮﮔ B
ﻲﻣ ﻥﺎﺸﻧ ﺪﻫﺩ
. ﺮﺘﺸﻴﺑ ﺺﻴﻠﺨﺗ ﺖﻬﺟ ﺍﺬﻟ
ﮐﺍﺮﻓ ﺶﻨ ﻱﺎﻫ ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻡﻭﺩ ﮏﻴﭘ ﺎﺑ ﺐﺳﺎﻨﺘﻣ )
ﻪﻧﻮﻤﻧ
(A ﻊﻤﺟ ﻆﻴﻠﻐﺗ ﺯﺍ ﺲﭘ ﻭ ﻱﺭﻭﺁ ,
ﻪﻴﻠﻋ ﺮﻓﺎﺑ ﻥﻮﺘﺳ ﺰﻴﻟﺎﻳﺩ
ﻳﺩﺮﮔ ﻭ ﻩﺪ ﻥﻮﺘﺳ ﺮﺑ ﺲﭙﺳ ﻥﻮﻳ ﺾﻳﻮﻌﺗ
Sp-Sepharose
ﻩﺩﺍﺩ ﺭﺍﺮﻗ ﻢﺳﺭ ﻪﻃﻮﺑﺮﻣ ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﻭ ﺪﺷ
ﺪﻳﺩﺮﮔ
) B1 ﻞﮑﺷ .(
ﮏﻴﭘ ﺲﭙﺳ ﻱﺎﻫ
ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﺯﺍ ﻞﺻﺎﺣ
ﻊﻤﺟ ﻱﺭﻭﺁ
، ﻆﻴﻠﻐﺗ
، ﻭ ﺰﻴﻟﺎﻳﺩ ﺯﺍ ﺲﭘ ﺖﻬﺟ ﺖﻈﻠﻏ ﻦﻴﻴﻌﺗ
ﺶﺠﻨﺳ ﺖﻴﻟﺎﻌﻓ ﻲﻳﺍﺰﮔﺭﺪﺿ ﺮﺑ
ﺀﺎﺸﻏ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ
ﻪﺟﻮﺟ ﺪﺷ ﻪﺘﻓﺮﮔ ﺭﺎﮑﺑ .
ﺍﻮﻧ ﺭﺩ ﻲﻳﺍﺰﮔﺭ ﻥﺍﺰﻴﻣ ﻑﺍﺮﻃﺍ ﻲﺣ
ﮏﺴﻳﺩ ﺯﺍ ﮏﻳ ﺮﻫ ﻞﻴﺘﻣ ﻱﺎﻫ
ﻪﻧﻮﻤﻧ ﻞﻣﺎﺣ ﺰﻟﻮﻠﺳ ﻱﺎﻫ
ﻩﻭﺮﮔ ﺎﺑ ﻪﺴﻳﺎﻘﻣ ﺭﺩ ﺶﻳﺎﻣﺯﺁ ﺩﺭﻮﻣ ﻲﻨﻴﺌﺗﻭﺮﭘ ﺪﻫﺎﺷ
ﺪﻳﺩﺮﮔ ﻪﺴﻳﺎﻘﻣ ﻭ ﻲﺑﺎﻳﺯﺭﺍ .
ﺭﺩ ﻪﮐ ﺭﻮﻄﻧﺎﻤﻫ ﻞﮑﺷ
) ۳ (
ﻲﻣ ﻩﺪﻫﺎﺸﻣ ﺩﺩﺮﮔ
ﻓﺎﻳﺭﺩ ﻩﻭﺮﮔ ﻩﺪﻨﻨﮐ ﺖ
ﻪﻧﻮﻤﻧ A1
ﺭﺩ
ﺪﻫﺎﺷ ﻩﻭﺮﮔ ﺎﺑ ﻪﺴﻳﺎﻘﻣ ﺖﻴﻟﺎﻌﻓ ﻦﻳﺮﺘﺸﻴﺑ
ﺪﺿ ﻲﻳﺍﺰﮔﺭ ﺍﺭ
ﻭ ﻪﺘﺷﺍﺩ ﻦﻳﺍ
ﺶﻨﮐﺍﺮﻓ ﺩ ﻊﻗﺍﻭ ﺭ ﻦﻴﺌﺗﻭﺮﭘ ﻱﻭﺎﺣ ﻝﺎﻌﻓ
ﺖﺳﺍ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﺭﺩ ﺩﻮﺟﻮﻣ .
ﻥﺎﻤﻫ ﻪﻧﻮﮔ ﻪﮐ
ﺭﺩ ﻞﮑﺷ ) ۴ ( ﻩﺪﻫﺎﺸﻣ ﻲﻣ ﺩﻮﺷ ﻪﻧﻮﻤﻧ ﻦﻴﺌﺗﻭﺮﭘ ﻱﻭﺎﺣA -
ﻥﺯﻭ ﺎﺑ ﻲﻳﺎﻫ ﻢﻈﻋﺍ ﺖﻤﺴﻗ ﻭ ﻩﺩﻮﺑ ﻉﻮﻨﺘﻣ ﻲﻟﻮﮑﻟﻮﻣ ﻱﺎﻫ
ﻦﻴﺌﺗﻭﺮﭘ ﺍﺮﻧﺁ ﻲﻣ ﻞﻴﮑﺸﺗ ﻻﺎﺑ ﻲﻟﻮﮑﻟﻮﻣ ﻥﺯﻭ ﺎﺑ ﻱﺎﻫ
ﺪﻫﺩ
ﻲﻟﺎﺣ ﺭﺩ ﻪﮐ ﻪﻧﻮﻤﻧ A1
ﻪﮐ ﻲﻳﺍﺰﮔﺭﺪﺿ ﺖﻴﻟﺎﻌﻓ ﻦﻳﺮﺘﺸﻴﺑ ﺍﺭ
ﺯﺍ ﻞﮑﺸﺘﻣ ﺖﺷﺍﺩ ﻲﻨﻴﺌﺗﻭﺮﭘ ﺪﻧﺎﺑ ﺖﻔﺟ ﮏﻳ
ﻭ ﮏﺒﺳ ﺎﺑ
ﺩﻭﺪﺣ ﻭ ﻦﻴﻳﺎﭘ ﻲﻟﻮﮑﻟﻮﻣ ﻥﺯﻭ ۱۶
‐ ۱۴ ﻥﻮﺘﻟﺍﺩﻮﻠﻴﮐ
ﻲﻣ ﺪﺷﺎﺑ .
ﺚﺤﺑ ﻪـﺳﻮﮐ ﻑﻭﺮـﻀﻏ ﺖﻓﺎﺑ ﺝﺍﺮﺨﺘﺳﺍ ﺯﺍ ﺲﭘ ﻖﻴﻘﺤﺗ ﻦﻳﺍ ﺭﺩ
ﻥﻮﻴﺗﺎـﮐ ﻭ ﻥﻮـﻴﻧﺁ ﺾﻳﻮﻌﺗ ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﺵﻭﺮﺑ ﻲﻫﺎﻣ
،
ﻦﻴﺌﺗﻭﺮﭘ ﻞﮐ ﺲﭙـﺳ ﻭ ﻩﺪـﻳﺩﺮﮔ ﮏﻴﮑﻔﺗ ﻪﻃﻮﺑﺮﻣ ﻱﺎﻫ
ﻪـﺑ
ﻪﻧﻮﻤﻧ ﺕﺭﻮﺻ ﺎـﻬﻧﺁ ﻲـﻳﺍﺰﮔﺭ ﺪـﺿ ﺮﻴﺛﺎـﺗ ﻪـﻧﺎﮔﺍﺪﺟ ﻲﻳﺎﻫ
ﻲﻳﺍﺮﺤـﺻ ﺵﻮـﻣ ﺕﺭﻮـﺋﺁ ﻲﻳﺍﺰﮔﺭ ﺶﺠﻨﺳ ﻝﺪﻣ ﻂﺳﻮﺗ Rat) ( ﺖـﻓﺮﮔ ﺭﺍﺮـﻗ ﻲﺑﺎـﻳﺯﺭﺍ ﺩﺭﻮﻣ .
ﺞﻳﺎـﺘﻧ ﻲـﺳﺭﺮﺑ ﺎـﺑ
ﻞﻣﺎـﺷ ﻲﻳﺎـﻬﻧ ﻝﺎـﻌﻓ ﻪﻧﻮﻤﻧ ﻪﮐ ﺪﻳﺩﺮﮔ ﺺﺨﺸﻣ ﻪﻠﺻﺎﺣ ﻲﻟﻮﮑﻟﻮﻣ ﻥﺯﻭ ﺎﺑ ﻦﻴﺌﺗﻭﺮﭘ ﺖﻔﺟ ﮏﻳ ۱۶
‐ ۱۴ ﻥﻮﺘﻟﺍﺩﻮﻠﻴﮐ
ﻲﻣ ﺪﺷﺎﺑ .
ﻲﻳﺍﺰﮔﺭ ﻩﺪﻳﺪﭘ ﺣ ﺭﺩ ﻪﮐ ﺖﺳﺍ ﻱﺍ ﺖﻟﺎ
ﺖﺤﺗ ﻲﻌﻴﺒﻃ ﺩﺮﮑﻠﻤﻋ
ﻢﺴﻴﻧﺎﮑﻣ ﻱﺎﻫ ﻝﺮﺘﻨﮐ ﻲﻌﻴﺒﻃ ﻟﻮﻠﺳ
ﻲ ﺖـﺳﺍ . ﻪـﮐ ﺪـﻨﭼ ﺮـﻫ
ﺖﻴﻟﺎﻌﻓ ﻲﻳﺍﺰﮔﺭ ﺖـﻟﺎﺣ ﺭﺩ ﺖـﻬﺟ ﻲـﻌﻴﺒﻃ
ﻭ ﺪـﺷﺭ ﺀﺎـﻘﺑ
ﻩﺪـﺸﻧ ﻝﺮﺘﻨﮐ ﺖﻟﺎﺣ ﻲﻟﻭ ﺖﺳﺍ ﻱﺭﻭﺮﺿ ﺩﻮﺟﻮﻣ ﻥﺁ
ﺮـﺠﻨﻣ
ﻪﺑ ﺯﺍ ﻲﻌﻴﺳﻭ ﻒﻴﻃ ﺯﻭﺮﺑ ﻱﺭﺎﻤﻴﺑ
ﻲـﻗﻭﺮﻋ ﻱﺎﻫ ﺹﻮـﺼﺨﺑ
ﻥﺎﻃﺮﺳ ﻉﺍﻮﻧﺍ ﻲﻣ
ﺩﺩﺮﮔ . ﺕﺎـﻘﻴﻘﺤﺗ ﺯﺍ ﻩﺪـﻣﺁ ﺖـﺳﺪﺑ ﺞﻳﺎـﺘﻧ
ﺎﻴﺑ ﻲﮑﻴﻨﻴﻠﮐ ﻪـﺑ ﻼﺘـﺒﻣ ﺩﺍﺮﻓﺍ ﻱﻻﺎﺑ ﺪﺻﺭﺩ ﻭ ﻊﻳﺮﺳ ﺪﺷﺭ ﺮﮕﻧ
ﻱﺭﺎﻤﻴﺑ ﺖﻴﻌﻤﺟ ﺭﺩ ﻲﻗﻭﺮﻋ ﻱﺎﻫ ﺖﺳﺍ ﻒﻠﺘﺨﻣ ﻱﺎﻫ
. ﻞﻣﺍﻮﻋ
ﻱﺭﺎﻤﻴﺑ ﻪﻧﻮﮔ ﻦﻳﺍ ﻞﻠﻋ ﻭ ﻝﻮﻠـﺳ ﺢﻄـﺳ ﺭﺩ ﺖـﻳﺎﻬﻧ ﺭﺩ ،ﺎـﻫ
ﻝﻮﻠـﺳ ﺩﺮـﮑﻠﻤﻋ ﺎـﺑ ﻂﺒﺗﺮﻣ ﻭ ﻩﺩﻮﺑ ﺡﺮﻄﻣ ﻝﺎﻴﻠﺗﻭﺪـﻧﺍ ﻱﺎـﻫ
ﺖﺳﺍ ﺮﻈﻧ ﺩﺭﻮﻣ ﺖﻓﺎﺑ ﻕﻭﺮﻋ ﻩﺪﻧﺯﺎﺳ .
ﻭ ﺪـﺷﺭ ﻝﺮـﺘﻨﮐ ﺍﺬـﻟ
ﺭﺎﻤﻴﺑ ﻪﻧﻮﮕﻨﻳﺍﺭﺩ ﻲﻟﻮﻠﺳ ﺮﻴﺜﮑﺗ ﻱ
ﻲﻧﺎـﻣﺭﺩ ﺩﺮﺑﺭﺎـﮐ ﻱﺍﺭﺍﺩ ﺎـﻫ
ﺖﺳﺍ . ﺖـﻬﺟ ﻱﺭﺎﻴـﺴﺑ ﻲﻋﻮﻨـﺼﻣ ﻭ ﻲـﻌﻴﺒﻃ ﻲﻳﻭﺭﺍﺩ ﺩﺍﻮﻣ
ﻝﻮﻠـﺳ ﺭﺩ ﻲـﻳﺍﺰﮔﺭ ﻪـﻳﻭﺭ ﻲﺑ ﺪﺷﺭ ﻝﺮﺘﻨﮐ ﻝﺎﻴﻠﺗﻭﺪـﻧﺍ ﻱﺎـﻫ
ﻩﺪﻳﺩﺮﮔ ﻲﺣﺍﺮﻃ ﻭ ﻲﻓﺮﻌﻣ ﺪﻧﺍ
. ﻭ ﺖﺧﺎﺳ ﺩﺮﮑﻳﻭﺭ ﻩﺯﻭﺮﻣﺍ ﺎﻣﺍ
ﻔﺘﺳﺍ ﺖﻬﺟ ﺭﺩ ﺮﺘﺸﻴﺑ ﻲﻧﺎﻣﺭﺩ ﻱﺎﻫﻭﺭﺍﺩ ﻲﺣﺍﺮﻃ ﻉﺍﻮﻧﺍ ﺯﺍ ﻩﺩﺎ
ﺖﺳﺍ ﻲﻌﻴﺒﻃ ﺀﺎﺸﻨﻣ ﺎﺑ )
۱۹ (.
ﺮﺘﻛﺩ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﯽﻫﺎﮔﺭﺎﺑ
ﺮﻴﺛﺄﺗ
ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﻦﻴﺌﺗﻭﺮﭘ
ﻪﺳﻮﻛ ﻑﻭﺮﻀﻏ ﯽﻫﺎﻣ
/ ۶
A
B
C
ﻞﮑﺷ ۲ : ﻪﻧﻮﻤﻧ ﺮﻴﺛﺄﺗ A ﻱﺎﻫ
Bﻭ ﺯﺍ ﻞﺻﺎﺣ ﻲﻫﺎـﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻩﺭﺎﺼﻋ
ﻲــﻳﺍﺰﮔﺭ ﻥﺍﺰــﻴﻣﺮﺑ ﻪــﺟﻮﺟ ﮏــﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎــﺸﻏ
A .
;Bﻭ ﺭﺩ ﺀﺎــﺸﻏ
ﺭﻮﻀﺣ ۱۰۰ ﻪـﺑ ﺯﺍ ﻞـﺻﺎﺣ ﻦﻴﺌﺗﻭﺮﭘ ﻡﺮﮔﻭﺮﮑﻴﻣ ﻡﻭﺩ ﻭ ﻝﻭﺍ ﮏـﻴﭘ ،ﺐـﻴﺗﺮﺗ
ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﺾﻳﻮﻌﺗ ﻥﻮﺘﺳ
ﻧﺁ ﻥﻮﻴ ; C . ﺮﻓﺎـﺑ ﺭﻮـﻀﺣ ﺭﺩ ﻝﺮـﺘﻨﮐ ﻪﻧﻮﻤﻧ
ﺕﺎﻔﺴﻓ . ﻩﺮﻳﺍﺩ ﺎﻫ ﮎﻼﭘ ﻱﺮﻴﮔﺭﺍﺮﻗ ﻲﺣﺍﻮﻧ ﺮﮕﻧﺎﻴﺑ ﻲﻣ ﺰﻟﻮﻠﺳ ﻞﻴﺘﻣ ﻱﺎﻫ
ﺪﻨﺷﺎﺑ
C A0
A1 A2
A3 A4
ﻞﮑﺷ ۳ : ﺮﻴﺛﺎﺗ ﻪﺴﻳﺎﻘﻣ ﻪﻧﻮﻤﻧ
ﻱﺎﻫ ﻲﻨﻴﺌﺗﻭﺮﭘ A0
A1، A2، A3، A4ﻭ ﺮـﺑ
ﻥﺍﺰﻴﻣ ﻲﻳﺍﺰﮔﺭ ﺭﺩ ﻪﺟﻮﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎﺸﻏ ﺭﺩ ﺖﻈﻠﻏ
ﺖـﺑﺎﺛ ۱۰۰
ﻦﻴـﻨﺟ ﺮﻫ ﺀﺍﺯﺍ ﻪﺑ ﻦﻴﺌﺗﻭﺮﭘ ﻡﺮﮔﻭﺮﮑﻴﻣ .
ﻪـﻧﻮﻤﻧ ﻫﺎـﺷ ﺮﻓﺎـﺑ ﺭﻮـﻀﺣ ﺭﺩ ﺪ
ﺕﺎﻔﺴﻓ . ﻩﺮﻳﺍﺩ ﮎﻼﭘ ﻱﺮﻴﮔﺭﺍﺮﻗ ﻲﺣﺍﻮﻧ ﺮﮕﻧﺎﻴﺑ ﺎﻫ ﻲـﻣ ﺰﻟﻮﻠـﺳ ﻞﻴﺘﻣ ﻱﺎﻫ
-
ﺪﻨﺷﺎﺑ .
ﻞﮑﺷ ۴ : ﺡﺮﻃ ﺯﺭﻮﻓﻭﺮﺘﮑﻟﺍ SDS-PAGE
ﻦﻴﺌﺗﻭﺮﭘ ﻪﺑ ﻁﻮﺑﺮﻣ ﻱﺎﻫ
ﻩﺪﺷ ﺝﺍﺮﺨﺘﺳﺍ ﻑﻭﺮﻀﻏ ﺯﺍ
ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﺮﺑ
ﻝﮊ ۱۲ ﺭﺩ ﺪﺻ ﻞﻳﺮﮐﺁ
،ﺪﻴﻣﺁ
ﻮﻠﺑ ﻲﺳﺎﻣﻮﮐ ﺎﺑ ﻩﺪﺷ ﻱﺰﻴﻣﺁ ﮓﻧﺭ R 250
M : ﻲﻟﻮﮑﻟﻮﻣ ﻥﺯﻭ ﺩﺭﺍﺪﻧﺎﺘﺳﺍ ؛
ﻦﻴﺌﺗﻭﺮﭘ . ﻒﻳﺩﺭ ﻱﺎﻫ ۱ ﻭ ۲ ﺑ ؛ ﻪ ﺐﻴﺗﺮﺗ ﻪﺑ ﻁﻮﺑﺮﻣ ﻪﻧﻮﻤﻧ ﻱﺎﻫ ﻲﻨﻴﺌﺗﻭﺮﭘ ﻭ A
.A0
ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﺭﺩ ﺭﺎﻴـﺴﺑ ﻥﺎﻃﺮـﺳ ﻪﺑ ﻼﺘﺑﺍ ﻝﺎﻤﺘﺣﺍ ﺎﻫ
ﻭ ﻢـﮐ
ﻥﻮﻴﻠﻴﻣ ﺭﺩ ﮏﻳ ﺩﻭﺪﺣ ﺭﺩ ﻲـﻓﺮﻃ ﺯﺍﻭ ﻩﺩﻮﺑ
ﺖﻴـﺻﻮﺼﺧ
ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﺐﻟﺎﺟ ﺩﻮﺟﻭ ﺎﻫ
ﹰﻼﻣﺎـﮐ ﺖﻠﮑـﺳﺍ ﻲﻓﻭﺮـﻀﻏ
ﺖﺳﺎﻬﻧﺁ ) ۲۰ .(
ﺎﺑ ﻪﮐ ﻲﺗﺎﻌﻟﺎﻄﻣ ﻪـﺑ ﻊﺟﺍﺭ
ﺪـﺿ ﺹﺍﻮـﺧ
ﻳﺍﺰﮔﺭ ﻡﺎﺠﻧﺍ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻲ
،ﺖﻓﺮﮔ ﻥﺍﺪﻨﻤﺸﻧﺍﺩ
ﺪﻧﺪﻴﺳﺭ ﻪﺠﻴﺘﻧ ﻦﻳﺍ ﻪﺑ ﻪﮐ
ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻱﻭﺎـﺣ
ﻦﻴﺌﺗﻭﺮﭘ ﺹﺍﻮﺧ ﺎﺑ ﻲﻳﺎﻫ ﺖﻴـﺻﺎﺧ ﺹﻮـﺼﺨﺑ ﻲـﻔﻠﺘﺨﻣ
ﺍﺰﮔﺭ ﺪﺿ ﻭ ﻩﺩﻮﺑ
ﺪﻧﺍﻮﺘﻴﻣ ﺵﻮـﺧ ﻱﺎـﻫﺭﻮﻣﻮﺗ ﺭﺎﻬﻣ ﺐﺒﺳ
ﻲﻣ ﻢﻴﺧ ﺪﺑﻭ ﻢﻴﺧ ﺩﺩﺮﮔ
. ﺐﺒـﺳ ﺞﻳﺎﺘﻧ ﻦﻳﺍ ﺡﺮـﻄﻣ
ﻥﺪـﺷ
ﺑ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻪ
ﻞـﻤﮑﻣ ﺯﺍ ﻲـﮑﻳ ﻥﺍﻮـﻨﻋ ﻱﺎـﻫ
ﺭﺍﺩ ﻲﻳﻭ ﺀﺎـﺸﻨﻣ ﺯﺍ ﻲـﻌﻴﺒﻃ
ﺪـﻳﺩﺮﮔ ) ۲۱ ( . ﻪـﺑ ﻪـﺟﻮﺗ ﺎـﺑ
،ﻲﻫﺎـﻣ ﻪـﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻲﻧﺎﻣﺭﺩ ﻭ ﻲﮑﻴﻨﻴﻠﮐ ﻱﺎﻫﺩﺮﺑﺭﺎﮐ ﻱﺭﺎﻤﻴﺑ ﻉﺍﻮﻧﺍ ﻥﺎﻣﺭﺩ ﺖﻬﺟ ﻥﺁ ﺮﺛﻮﻣ ﺩﺍﻮﻣ ﺝﺍﺮﺨﺘﺳﺍ ﻱﺎـﻫ
ﻲﻣ ﺖﻴﻤﻫﺍ ﺰﺋﺎﺣ ﻥﺎﻃﺮﺳ ﻭ ﻲﻗﻭﺮﻋ ﺪﺷﺎﺑ
) ۲۲ .(
ﻦـﻳﺍ ﺮـﺑ
۷ / ﺏﻮﻨﺟ ﺐﻃ
ﻝﺎﺳ
ﻢﻫﺩ ﻩﺭﺎﻤﺷ ۱ / ﺭﻮﻳﺮﻬﺷ ۱۳۸۶
ﻪـﮐ ﺪـﻳﺩﺮﮔ ﻲﻌـﺳ ﻖﻴﻘﺤﺗ ﻦﻳﺍ ﺭﺩ ﺱﺎﺳﺍ ﻦﻴﺌﺗﻭﺮـﭘ
ﻱﺎـﻫ
ﺍﺪـﺟ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻱﺍﺰﮔﺭ ﺪﺿ ﻝﺎﻌﻓ ﻭ ﻱﺯﺎـﺳ
ﺩﺩﺮﮔ ﻲﺑﺎﻳﺯﺭﺍ ﺎﻬﻧﺁ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺖﻴﻟﺎﻌﻓ .
ﺺﻴـﻠﺨﺗ ﺖﻬﺟ
ﻥﻮﻴﻧﺁ ﺾﻳﻮﻌﺗ ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ ﺵﻭﺭ ﺯﺍ Q-Sepharose
ﻪـﻧﻮﮔ ﻦـﻳﺍ ﺭﺩ ﮏﻴﮑﻔﺗ ﺖﻗﺩ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻪﮐ ﺪﺷ ﻩﺩﺎﻔﺘﺳﺍ ﮏﻴﭘ ﻭﺩ ﻪﺑ ﹰﻼﻣﺎﮐ ﻪﻴﻟﻭﺍ ﻩﺭﺎﺼﻋ ،ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ B)
(Aﻭ
ﺪﻳﺩﺮﮔ ﻚﻴﻜﻔﺗ )
A1ﻞﻜﺷ ( . ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺮﻴﺛﺄﺗ ﻲﺳﺭﺮﺑ ﺎﺑ
ﻪﻧﻮﻤﻧ ﻮﮐﺬﻣ A ﺭ ﺭﺩ ﻪـﺟﻮﺟ ﮏـﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺀﺎـﺸﻏ ﺮﺑ
ﻞﮑﺷ ۲ ﻦﻴﺌﺗﻭﺮﭘ ﻪﮐ ﺪﺷ ﻪﺠﻴﺘﻧ ﻝﻭﺍ ﮏﻴﭘ ﺭﺩ ﺩﻮﺟﻮﻣ ﻱﺎﻫ
ﻡﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ )
ﻪﻧﻮﻤﻧ (A ﻩﻭﺮـﮔ ﺎـﺑ ﻪﺴﻳﺎﻘﻣ ﺭﺩ ﺯﺍ ﺪﻫﺎـﺷ
ﺭﺍﺩﺭﻮـــﺧﺮﺑ ﻱﺮﺘـــﺸﻴﺑ ﻲـــﻳﺍﺰﮔﺭ ﺪـــﺿ ﺖـــﻴﻟﺎﻌﻓ ﺖﺳﺍ ) ۰۱۳ / P<۰ .(
ﻦﻴﺌﺗﻭﺮـﭘ ﻪـﮐ ﺪﺷ ﻪﺘﻓﺮﮔ ﻪﺠﻴﺘﻧ ﺍﺬﻟ
ﺀﺰﺟ ﺍﺰﮔﺭﺪﺿ Aﻚﻴﭘ
ﻦﻴﺌﺗﻭﺮـﭘ ﻭ ﻩﺩﻮﺑ ﺩﻮـﺟﻮﻣ ﻱﺎـﻫ
ﻂﻳﺍﺮﺷ ﺖﺤﺗ ﻪﺘـﺳﺩ ﺀﺰـﺟ ﻥﻮﺘـﺳ ﺭﺩ ﻪـﺘﻓﺭ ﺭﺎـﮑﺑpH
ﻦﻴﺌﺗﻭﺮــﭘ ﻲــﻣ ﺖــﺒﺜﻣ ﺭﺎــﺑ ﺎــﺑ ﻱﺎــﻫ ﺪﻨــﺷﺎﺑ
. ﻝﮊ ﺰﻴﻟﺎــﻧﺁ
ﻪﻧﻮﻤﻧ ﺯﺭﻮﻓﻭﺮﺘﮑﻟﺍ ﺗ ﺩﻮﺟﻭ ﺮﮕﻧﺎﻴﺑA
ـ ﺤﻣ ﺩﺍﺪﻌ ــ ﻱﺩﻭﺪ
ﻦﻴﺌﺗﻭﺮﭘ ﺖﺳﺍ ﻦﻴﻳﺎﭘ ﻲﻟﻮﮑﻟﻮﻣ ﻥﺯﻭ ﺎﺑ )
ﻲـﻌﻴﺒﻃ ﺭﻮﻃ ﻪﺑ
ﻝﻮﮑﻟﻮﻣﻭﺮﮐﺎﻣ ﺪﺻﺭﺩ ﺭﺩ ﻦﻴﻳﺎـﭘ ﻲﻟﻮـﮑﻟﻮﻣ ﻥﺯﻭ ﺎﺑ ﻱﺎﻫ
ﺮـﺘﻤﮐ ﻩﺪﻧﺯﺎـﺳ ﺩﺍﻮﻣﺮﻳﺎـﺳ ﻪـﺑ ﺖﺒـﺴﻧ ﻑﻭﺮـﻀﻏ ﺖﻓﺎﺑ ﻫ ﺪﻨﺘﺴ ) ( ﻞﮑﺷ ۴ .(
ﻪـــﻧﻮﻤﻧ ،ﺮﺘـــﺸﻴﺑ ﺺﻴـــﻠﺨﺗ ﺖـــﻬﺟ ﻥﻮﺘـــﺳ ﺮـــﺑA
ﻥﻮﻴﺗﺎﮐ ﺾﻳﻮﻌﺗ ﻲﻓﺍﺮﮔﻮﺗﺎﻣﻭﺮﮐ Sp-Sepharose
ﺭﺍﺮﻗ
ﮏﻴﭘ ﻭ ﺪﺷ ﻩﺩﺍﺩ ﻱﺎﻫ
A4) A0- ( ﺯﺍ ﺮـﻳﺯ ﻥﻮﺘـﺳ ﻊـﻤﺟ
ﺪﻳﺩﺮﮔ ﻱﺭﻭﺁ )
Bﻞﮑﺷ ۱ .(
ﻪـﻧﻮﻤﻧ ﺯﺍ ﮏـﻳﺮﻫ ﹰﺍﺩﺪﺠﻣ ﺎـﻫ
ﺖﻬﺟ ﺖـﻴﻟﺎﻌﻓ ﻲﺑﺎـﻳﺯﺭﺍ ﻲﻳﺍﺰﮔﺭﺪـﺿ
ﻝﺪـﻣ ﺭﺩ ﺀﺎـﺸﻏ
ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺪﻫﺎـﺷ ﻩﻭﺮـﮔ ﺎـﺑ ﻪﺴﻳﺎﻘﻣ ﺭﺩ
ﺭﺎـﮐ ﻪـﺑ
ﻪﮐ ﺪﺷ ﻪﺘﻓﺮﮔ ﻦﻳﺮﺘﺸﻴﺑ
ﺖﻴﻟﺎﻌﻓ ﻲﻳﺍﺰﮔﺭﺪﺿ ﻪـﺑ ﻁﻮﺑﺮﻣ
ﻪــــﻧﻮﻤﻧ A1
ﺩﻮــــﺑ ) ۰۱۶ / P<۰ .(
ﺀﺎــــﺸﻏ ﺍﺮــــﻳﺯ
ﻦﻴﻨﺟ ﮏﻴﺋﻮﺘﻧﻻﺁﻮﻳﺭﻮﮐ ﺶﻫﺎـﮐ ﻪـﻧﻮﻤﻧ ﺮﻴﺛﺄﺗ ﺖﺤﺗ ﻱﺎﻫ
ﺪـﺣﺍﻭ ﺭﺩ ﻕﻭﺮـﻋ ﺮـﻄﻗ ﻭ ﺩﺍﺪـﻌﺗ ،ﻩﺯﺍﺪﻧﺍ ﺮﻈﻧ ﺯﺍ ﺪﻳﺪﺷ ﺶﻤﺧ ﺩﺍﺪﻌﺗ ،ﺢﻄﺳ ﮒﺭ ﺕﺎﺑﺎﻌﺸﻧﺍ ،ﺎﻫ
ﺪـﻧﺩﺍﺩ ﻥﺎـﺸﻧ ﺎﻫ )
ﻞﮑﺷ ۳ .(
ﺼﻋ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺮﻴﺛﺄﺗ ﻦﻳﺍﺮﺑﺎﻨﺑ ﻪﻴﻬﺗ ﻪﻴﻟﻭﺍ ﻩﺭﺎ
ﺮﺸﺘﻨﻣ ﻲﻤﻠﻋ ﺕﺎﺷﺭﺍﺰﮔ ﺎﺑ ﻖﻓﺍﻮﺗ ﺭﺩ ﻩﺎﮕﺸﻳﺎﻣﺯﺁ ﺭﺩ ﻩﺪﺷ ﻩﺪﺷ ﻪـﻤﻴﻧ ﻭ ﻪـﻴﻟﻭﺍ ﻩﺭﺎـﺼﻋ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺮﻴﺛﺄﺗ ﺮﺑ ﻲﻨﺒﻣ
ﺺﻟﺎﺧ ﻪـﺳﻮﮐ ﻑﻭﺮـﻀﻏ ﺪـﻴﻳﺄﺗ ﹰﺍﺩﺪـﺠﻣ ﻲـﻳﺍﺰﮔﺭ ﺮـﺑ
ﺪــﻳﺩﺮﮔ ) ۲۶ ‐ ۲۳ .(
ﺖــﻬﺟ ﻦﻴــﻴﻌﺗ ﻲــﻨﻴﺌﺗﻭﺮﭘ ﻱﻮــﺘﺤﻣ
ﻪﻧﻮﻤﻧ ﻱﺎﻫ ﻝﺎﻌﻓ ﻭA A1
ﺰﻴﻟﺎﻧﺁ ﻓﻭﺮﺘﮑﻟﺍ ﻝﮊ ﺭﻮ
SDS- ﺯ
ﻪﮐ ﺪﺷ ﻡﺎﺠﻧﺍPAGE ﻮﻤﻧ
A1 ﻪﻧ ﺎﺑ ﺖـﻴﻟﺎﻌﻓ ﻦﻳﺮﺘـﺸﻴﺑ
ﻲﻳﺍﺰﮔﺭﺪﺿ ﺯﺍ ﻞﮑﺸﺘﻣ
ﻲﻨﻴﺌﺗﻭﺮﭘ ﺪﻧﺎﺑ ﺖﻔﺟ ﮏﻳ ﻥﺯﻭ ﺎﺑ
ﻲﻟﻮــﮑﻟﻮﻣ ۱۶
‐ ۱۴ ﺩﻮــﺑ ﻥﻮــﺘﻟﺍﺩﻮﻠﻴﮐ
) ﻞﮑــﺷ ۴ ( . ﻲــﻟﻭ
ﻮــﺷ ﺵﺭﺍﺰــﮔ ﻑﻼﺧﺮــﺑ Sheu)
( ﻝﺎــﺳ ﺭﺩ ۱۹۹۸
ﻭ
ﮓـﻧﺎﻴﻟ ﺵﺭﺍﺰﮔ ﻦﻴﭽﻤﻫ Liang)
( ﮓـﻧﻭ ﻭ Wong)
(
ﻝﺎﺳ ﺭﺩ ۲۰۰۰ ﻦـﻳﺍ ﺯﺍ ﻞـﺻﺎﺣ ﺯﺭﻮﻓﻭﺮﺘﮑﻟﺍ ﻝﮊ ﻱﻮﮕﻟﺍ
ﺯﺍ ﻩﺪﻣﺁ ﺖﺳﺪﺑ ﺞﻳﺎﺘﻧ ﻭ ﻖﻴﻘﺤﺗ ﺶﻳﺎﻣﺯﺁ
ﺶﺠﻨـﺳ ﻱﺎـﻫ
ﻪــﻃﻮﺑﺮﻣ ﻲــﻳﺍﺰﮔﺭ ﻦﻴﺌﺗﻭﺮــﭘ ﺩﻮــﺟﻭ ﻡﺪــﻋ ﺮﮕﻧﺎــﻴﺑ
۱۰
ﻑﻭﺮـﻀﻏ ﺭﺩ ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﺹﺍﻮﺧ ﻱﺍﺭﺍﺩ ﻲﻧﻮﺘﻟﺍﺩﻮﻠﻴﮐ ﺖﺳﺍ ﻲﻫﺎﻣ ﻪﺳﻮﮐ
) ۲۷ ( .
ﻲـﻣ ﻖـﻴﻘﺤﺗ ﻦﻳﺍ ﺯﺍ ﻩﺪﻣﺁ ﺖﺳﺩ ﻪﺑ ﺞﻳﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻥﺍﻮـﺗ
ﻭ ﻲـﻨﻏ ﻊﺒﻨﻣ ﮏﻳ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻑﻭﺮﻀﻏ ﻪﮐ ﺖﻓﺮﮔ ﻪﺠﻴﺘﻧ ﭘ ﺕﺎﺒﻴﮐﺮﺗ ﻪﻠﻤﺟ ﺯﺍ ﺍﺰﮔﺭ ﺪﺿ ﻱﺎﻫﺭﻮﺘﮐﺎﻓ ﺯﺍ ﻱﻮﻗ ﻲﻨﻴﺌﺗﻭﺮ
ﺪـﻨﻣﺯﺎﻴﻧ ﺎـﻬﻧﺁ ﺮﺘﺸﻴﺑ ﮏﻴﮑﻔﺗ ﻪﮐ ﻩﺩﻮﺑ ﻢﮐ ﻲﻟﻮﮑﻟﻮﻣ ﻥﺯﻭ ﺎﺑ ﻲﺳﺭﺮﺑ ﺵﻭﺭ ﺯﺍ ﻩﺩﺎﻔﺘﺳﺍ ﻭ ﺮﺘﺸﻴﺑ ﻱﺎﻫ ﻱﺯﺎـﺳ ﺍﺪـﺟ ﻱﺎـﻫ
ﻪﺘﻓﺮﺸﻴﭘ ﺖـﺳﺍ ﻱﺮﺗ .
ﻦـﻳﺍ ﺮﺘـﺸﻴﺑ ﺺﻴـﻠﺨﺗ ﺕﻼﮑـﺸﻣ ﺯﺍ
ﻦﻴﺌﺗﻭﺮﭘ ﺰﻧ ﺎﻫ ﺩ ﺎـﺑ ﻦﻴـﻨﭽﻤﻫ ﻭ ﻲﻟﻮـﮑﻟﻮﻣ ﻥﺯﻭ ﻥﺩﻮـﺑ ﮏﻳ
ﻡﺍﺮﮔﻮﺗﺎـﻣﻭﺮﮐ ﺯﺍ ﻩﺪـﻣﺁ ﺖـﺳﺩ ﻪـﺑ ﺞﻳﺎـﺘﻧ ﻭ ﻮﮕﻟﺍ ﻪﺑ ﻪﺟﻮﺗ ﺎﻬﻧﺁ ﻥﻮﻳ ﺾﻳﻮﻌﺗ
، ﺖـﻴﺒﻄﻗ ﻭ ﻲـﮑﻳﺮﺘﮑﻟﺍ ﺭﺎﺑ ﻥﺩﻮﺑ ﻥﺎﺴﮑﻳ
ﺎﻬﻧﺁ ﺍ ﺖﺳ .
ﺭﻮﻓﻭ ﺰﻴﻧ ﻭ ﻱﻮﻗ ﻲﻳﺍﺰﮔﺭﺪﺿ ﺹﺍﻮﺧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺍﺬﻟ ﺏﺁ ﺭﺩ ﻲﻫﺎﻣ ﻪﺳﻮﮐ ﻥﺍﻭﺍﺮﻓ ﻭ ﻲﻌﻴﺒﻃ ﻦﻳﺍ ﻥﺍﺮﻳﺍ ﻡﺮﮔ ﻱﺎﻫ
ﻲﻣ ﻦﻴﺌﺗﻭﺮﭘ ﺵﻭﺭ ﻱﺮﻴﮔﺭﺎﮐ ﻪﺑ ﺎﺑ ﺪﻧﺍﻮﺗ
ﻲﺳﺪﻨﻬﻣ ﻱﺎﻫ
ﺍﺰﮔﺭﺪﺿ ﻱﺪﻴﺘﭙﭘ ﻱﻭﺭﺍﺩ ﻲﻋﻮﻧ ﻲﺣﺍﺮﻃ ﺖﻬﺟ ﮏﻴﺘﻧﮊ ﻪﺑ ﻲﻧﺎﻣﺭﺩ ﻑﺍﺪﻫﺍ ﺖﻬﺟ ﻮﺷ ﻪﺘﻓﺮﮔ ﺭﺎﮐ
ﺩ .
ﺮﺘﻛﺩ ﻥﺍﺭﺎﮑﻤﻫ ﻭ ﯽﻫﺎﮔﺭﺎﺑ
ﺮﻴﺛﺄﺗ
ﻲﻳﺍﺰﮔﺭ ﺪﺿ ﻦﻴﺌﺗﻭﺮﭘ
ﻪﺳﻮﻛ ﻑﻭﺮﻀﻏ ﯽﻫﺎﻣ
/ ۸
References: __________________________________________________________
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