ناﺮﻳاﻲﺳﺎﻨﺷﺖﺴﻳزﻲﻠﻠﻤﻟاﻦﻴﺑﺲﻧاﺮﻔﻨﻛﻦﻴﻤﺠﻨﭘويﺮﺳاﺮﺳﺲﻧاﺮﻔﻨﻛﻦﻴﻤﻫﺪﻔﻫ 16
- رﻮﻳﺮﻬﺷ14 نﺎﻣﺮﻛﺮﻨﻫﺎﺑﺪﻴﻬﺷهﺎﮕﺸﻧاد91
و ﻲﻳﺎﺳﺎﻨﺷ ﺶﺠﻨﺳ
يﺪﻴﻳﻮﻧووﻼﻓ و ﻲﻠﻨﻓ تﺎﺒﻴﻛﺮﺗ رد
ﻞﮔ ﻦﻳذآ يﺎﻫ ﺖﻴﻌﻤﺟ ﻲﺧﺮﺑ ﻪﻧﻮﮔ ﻪﺳ
ناﺮﻳا ناردﺎﻣﻮﺑ
ر ﻪﻧﺎﺤﻳ رﻮﭘﻲﻳﺎﻧاد
1
،*
ﻃ ﻪﺒﻴ نﺎﻴﺒﺟر ﻋ،1
ارﺬ ارﻮﺒﺻ
،2
دﻮﻌﺴﻣ هدازﻲﻘﺗ .1
ﺮﻐﺻا ﻲﻧاﺮﻣﺎﻛ
1
1 اد- ﻪﺘﺧﻮﻣآﺶﻧ ﻪﺘﺷر
ﻲﺳﺎﻨﺷﺖﺴﻳز
، ناﺮﻬﺗ،ﺪﻫﺎﺷهﺎﮕﺸﻧاد
، ناﺮﻳا
2 هوﺮﮔرﺎﻳدﺎﺘﺳا- ﻲﺳﺎﻨﺷﺖﺴﻳز
، ناﺮﻬﺗ،ﺪﻫﺎﺷهﺎﮕﺸﻧاد
، ناﺮﻳا
3 ﻲﺳﺎﻨﺷﺖﺴﻳزهوﺮﮔرﺎﻳدﺎﺘﺳا-
، ناﺮﻬﺗ،ءاﺮﻫﺰﻟاهﺎﮕﺸﻧاد
، ناﺮﻳا
E-mail:[email protected]
هﺪﻴﻜﭼ
فﺪﻫ ﺶﻫوﮋﭘ ﻦﻳا راﺪﻘﻣ ﻦﻴﻴﻌﺗ
ﺪﻴﻳﻮﻧووﻼﻓ و ﻞﻨﻓ ﻞﻛ
زا ﻲﻋاﻮﻧا ﻲﻳﺎﺳﺎﻨﺷ و ﺎﻫﺪﻴﻳﻮﻧووﻼﻓ
ﻦﻳذآ ﻞﮔ رد يﺎﻫ ﺖﻴﻌﻤﺟ ﻲﺧﺮﺑ
ﻪﻧﻮﮔ 3
ناردﺎﻣﻮﺑ
Achillea)
( نﺎﮔرﺎﺘﺳ ﻞﮔ ) ناﺮﻳا (
دﻮﺑ ﻪﻧﻮﮔ راﺪﻠﮔ يﺎﻫ ﻪﻧﻮﻤﻧ رﻮﻈﻨﻣ ﻦﻳﺪﺑ . يﺎﻫ
A. millefolium ﺖﻴﻌﻤﺟ )
يﺎﻫ و ناﺰﻳد
ناﺮﻤﻴﻣ
، ( A. wilhelmsii ﺖﻴﻌﻤﺟ )
ﻦﻳوﺰﻗ و نﺎﺷﺎﻛ و (
A. tenuifolia ﺖﻴﻌﻤﺟ )
يﺎﻫ و ناﺮﻬﺗ ﻢﻗ ﻫ هﺎﮕﺸﻳور زا ( ﻲﻌﻴﺒﻃ يﺎ
ﺪﺷ يروآ ﻊﻤﺟ هرﺎﺼﻋ .
يﺮﻴﮔ و ﻞﻨﻓ راﺪﻘﻣ و ﺪﺷ مﺎﺠﻧا لﻮﻧﺎﺗا ﺎﺑ ﺎﻫ ﻪﻧﻮﻤﻧ زا ﻞﻛ ﺪﻴﻳﻮﻧووﻼﻓ
ﻪﺑ ﺎﻫ نآ رد شور
يرﻮﻧ ﻲﺠﻨﺳ ﻒﻴﻃ
ﺪﺷ ﻦﻴﻴﻌﺗ زا ﻲﻋاﻮﻧا دﻮﺟو .
ﺎﻫﺪﻴﻳﻮﻧووﻼﻓ شور ﻪﺑ ﺎﻫ هرﺎﺼﻋ رد
ﺮﻛ و كزﺎﻧ ﻪﻳﻻ ﻲﻓاﺮﮔﻮﺗﺎﻣ
TLC)
شور ﻪﺑ ﺎﻫ نآ راﺪﻘﻣ و ﺪﺷ ﺪﻴﻳﺎﺗ (
ﺮﻛ و ﻊﻳﺎﻣ ﻲﻓاﺮﮔﻮﺗﺎﻣ ﺎﺑ
ﻲﻳارﺎﻛ ﻻﺎﺑ
HPLC) ﺪﺷ ﺶﺠﻨﺳ (
ﻮﺘﺤﻣ ﻦﻳﺮﺘﺸﻴﺑ . ا
ﻞﻛ ﻞﻨﻓ ي
mg/g DW)
20 /
±
2 60 / 52 رد ( ﻦﻳذآ ﻞﮔ
A. millefolium ناﺰﻳد ﺖﻴﻌﻤﺟ )
و ( ﻦﻳﺮﺘﺸﻴﺑ ﻮﺘﺤﻣ
ا ﺪﻴﻳﻮﻧووﻼﻓ ي ﻞﻛ
mg/g DW
) 11
/
±
0 40 / 5 رد ( ﻦﻳذآ ﻞﮔ
A. wilhelmsii ﻦﻳوﺰﻗ ﺖﻴﻌﻤﺟ )
ﺪﻣآ ﺖﺳﺪﺑ ( ﺮﺑ .
هداد سﺎﺳا يﺎﻫ
راﺪﻘﻣ
HPLCﺎﻫﺪﻴﻳﻮﻧووﻼﻓ
ي
،ﻦﻴﺘﺳﺮﺋﻮﻛ ﻲﭘا
ﻪﺑ ﻦﻴﺗور و ﻦﻴﻧژ
رد ﺐﻴﺗﺮﺗ يﺎﻫ هزﺎﺑ
61 / 0 - 01 /
، 0 61 / 1 - و 0
mg/g DW95
/ 0 - ﺎﻫ ﻪﻧﻮﻤﻧ رد 0 دﻮﺑ ﺮﻴﻐﺘﻣ
ﻦﻳﺮﺘﺸﻴﺑ . راﺪﻘﻣ ﻲﭘا و ﻦﻴﺘﺳﺮﺋﻮﻛ رد ﻦﻴﻧژ
ﻦﻳذآ ﻞﮔ A. millefolium
ناﺰﻳد ﺖﻴﻌﻤﺟ ) ﻪﻧﻮﮔ رد ﻦﻴﺗور و (
A. wilhelmsii ﻦﻳوﺰﻗ ﺖﻴﻌﻤﺟ )
ﺪﻣآ ﺖﺳﺪﺑ ( ﻪﻧﻮﻤﻧ رد .
درﻮﻣ يﺎﻫ
ﺑ ﺖﺷاﺪﻧ دﻮﺟو لوﺮﻔﻣﺎﻛ ﻲﺳرﺮ .
تﺎﻤﻠﻛ ﺪﻴﻠﻛ ي
،ﺪﻴﻳﻮﻧووﻼﻓ ،ناردﺎﻣﻮﺑ : يرﻮﻧ ﻲﺠﻨﺳ ﻒﻴﻃ
، ﺮﻛ و كزﺎﻧ ﻪﻳﻻ ﻲﻓاﺮﮔﻮﺗﺎﻣ
، ﺮﻛ و ﺎﺑ ﻊﻳﺎﻣ ﻲﻓاﺮﮔﻮﺗﺎﻣ ﻲﻳارﺎﻛ
ﻻﺎﺑ
The 17th National & 5th International Iranian Biology Cnference Septamber 4-6, 2012 Shahid Bahonar University of Kerman
Characterization and Determination of Phenolic and Flavonoid compounds in Inflorescence of some Populations from Three Iranian Achillea Species
R. Danaeipour1*, T. Radjabian1, A. Saboora2, M. Taghizadeh1, A. Kamrani1 1-Department of Biology, Shahed University, Tehran, Iran
2-Department of Biology, Alzahra University, Tehran, Iran Abstract
The aim of this study was determination of total phenolic and flavonoid content and characterization of some flavonoids in inflorescence of three Iranian Achillea (Asteraceae) species. Flowering samples of A.
millefolium (Dizan and Meimeran populations), A. wilhelmsii (Kashan and Qazvin populations) and A.
tenuifolia (Tehran and Qazvin populations) were collected from different natural regions. Samples were extracted in ethanol and total phenolic and flavonoid contents of extracts were determined spectrophotometrically. The existence of some flavonoids in samples was demonstrated by thin layer chromatography (TLC) method and their contents were determined by high performance liquid chromatoghraphy technique. The highest total content (52.60±2.20 mg/g DW) of phenolic compounds was obtained for inflorescence sample of A. millefolium (Dizan population) and the highest amount (5.40±0.11 mg/g DW) of total flavonoids was determined for inflorescence sample of A. wilhelmsii (Qazvin population). On the basis of HPLC data the amounts of quercetin, apigenin and rutin flavonoids in the samples ranged 1.01-0.61, 0-1.61 and 0-0.95 mg/g DW, respectively. The highest content of quercetin was calculated in inflorescence of A. millefolium (Dizan population) and the highest amount for rutin was obtained in inflorescence of A. wilhelmsii (Qazvin population). In this survey, kaempferol was not found in samples.
Key words: Achillea, Flavonoid, spectrophotometry, TLC, HPLC
