ﻞﯿﺑدرا رد هﺪﺷ ﯽﺷوﺮﻓ هدﺮﺧ ﺪﺠﻨﺳ ردﻮﭘ رد ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ
ﻞﺻا يدﺎﻨﻗ ﻪﻤﻃﺎﻓ
1
ﯽﯾﻮﻠﭼﺎﭼآ ﯽﺤﺘﻓ ماﺮﻬﺑ ،*
2
1 - رﺎﯾدﺎﺘﺳا
، ناﺮﯾا ،ﻞﯿﺑدرا ،ﯽﻠﯿﺑدرا ﻖﻘﺤﻣ هﺎﮕﺸﻧاد ،ﯽﻌﯿﺒﻃ ﻊﺑﺎﻨﻣ و يزروﺎﺸﮐ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ .
2 - ناﺮﯾا ،ﻞﯿﺑدرا ،ﯽﻠﯿﺑدرا ﻖﻘﺤﻣ هﺎﮕﺸﻧاد ،ﯽﻌﯿﺒﻃ ﻊﺑﺎﻨﻣ و يزروﺎﺸﮐ هﺪﮑﺸﻧاد ،ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ،رﺎﯿﺸﻧاد .
تﺎﻋﻼﻃا ﻪﻟﺎﻘﻣ
ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺦ ﺎﻫ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 05 / 05 / 98
رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 01 / 07 /
99
تﺎﻤﻠﮐ ﻠﮐ ﯿ ﺪ ي :
،ﻦﯿﺴﮐﻮﺗﻼﻓآ
،ﻻﺎﺑ ﯽﯾارﺎﮐ ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ نﻮﺘﺳ ﺎﺑ ﺺﯿﻠﺨﺗ ﻮﻧﻮﻤﯾا يﺎﻫ
،ﯽﺘﯿﻨﯿﻓا
ﺪﺠﻨﺳ ردﻮﭘ .
DOI: 10.29252/fsct.18.01.08 لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ : [email protected]
ﺎﻣ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿ ﻦ ﺎﻫ نﺎﻃﺮﺳ داﻮﻣ ناﻮﻨﻋ ﻪﺑ ﺴـﮐﻮﺗﻮﻧژ ،نژﻮـﺗاﺮﺗ ،از
ﯿ
،ﮏ ﺴـﮐﻮﺗﻮﺗﺎﭙﻫ ﯿ
ﮏ ﺴـﮐﻮﺗوﺮﻔﻧ و ﯿ
ﮏ
هﺪﺷ شراﺰﮔ ﺪﻧا
. روﺮﻣ تﺎﻌﻟﺎﻄﻣ ﻪﺑ ﺎﻨﺑ ي
مﺎﺠﻧا ﯾ
،ﻪﺘﻓﺎ ﺑ تﺎﻌﻟﺎﻄﻣ مﺎﺠﻧا ﯿ
ﺮﺘﺸ ﻌﺗ رﻮـﻈﻨﻣ ﻪـﺑ ﯿـﯿ
ﻦ ﮔدﻮـﻟآ ﯽ
ﺎﻣ ﯾ ﺴﮐﻮﺗﻮﮑ ﯿﻨ ﯽ ﻧا ﻪﻤﻫ رد اﺬﻏ داﻮﻣ عاﻮ
ﯽﯾ دراد دﻮﺟو .
،ﺮـﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد 20
ﺠﻨـﺳ ردﻮـﭘ ﻪـﻧﻮﻤﻧ ﺪ
ﻪـﺑ
ﻓدﺎﺼﺗ ترﻮﺻ ﯽ
- ﻪﺷﻮﺧ ا ي ﮔﺪﻨﮐاﺮﭘ سﺎﺳا ﺮﺑ و ﯽ
ﻞﺤﻣ ﺎﻫ ي ﺣاﻮﻧ زا شوﺮﻓ ﯽ
ﺑدرا ﺮﻬـﺷ ﻒـﻠﺘﺨﻣ ـﯿ
ﻞ
ﻬﺗ ﯿﻪ ﺪﺷ . ﻌﺗ ﯿﯿ ﻦ ﮔدﻮﻟآ ﯽ ﺴﮐﻮﺗﻼﻓآ ﯿﻨ ﯽ ﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ زا هدﺎﻔﺘﺳا ﺎﺑ ﯽ
ﺎﻣ ﯾ ﻊ آرﺎﮐ ﺎﺑ ﯽﯾ زﺎـﺳ ﺺﻟﺎﺧ و ﻻﺎﺑ ي
ا نﻮﺘﺳ ﺎﺑ ﯾ ﻓاﻮﻧﻮﻤ ﯿﻨﯿ ﺘ ﯽ ﺖﻓﺮﮔ ترﻮﺻ .
ﺎﺘﻧ ﻪﺑ ﺎﻨﺑ ﯾ
،ﺞ ﻪﻧﻮﻤﻧ ﻞﮐ زا ﻪﻧﻮﻤﻧ هﺪﻔﻫ ﺎـﻫ
) 85 ـﺻرد ﺪ ( ﮔدﻮـﻟآ ﯽ
ﺴﮐﻮﺗﻼﻓآ ﯿﻨ ﯽ ﻪﻨﻣاد ﺎﺑ 0 / 2360 - 40 / 30 ﻣ ﯿ مﺮﮔوﺮﮑ ﮐ رد ﯿ مﺮﮔﻮﻠ ﺪﻨﺘﺷاد . ﺴﮐﻮﺗﻼﻓآ ﯿ ﻦ ﺎـﻫ ي و B1 رد B2
85 ﻪﻧﻮﻤﻧ ﺪﺻرد ﺪﺷ هﺪﻫﺎﺸﻣ ﺎﻫ
. ﮔدﻮﻟآ ﯽ ﺴﮐﻮﺗﻼﻓآ ﯿ ﻦ ﺎﻫ ي و G1 ﺗﺮﺗ ﻪﺑ G2 ـﯿ ﺐ رد 80 و 70 ﺪـﺻرد
ﻪﻧﻮﻤﻧ ﺷ شراﺰﮔ ﺎﻫ ﺪ
. ﮔدﻮﻟآ ﺢﻄﺳ ﯽ
ا زﺎﺠﻣ ﺮﺜﮐاﺪﺣ ﺮﺑ نوﺰﻓا ﯾ
ناﺮ دﺎﺤﺗا و ﯾﻪ ﻮﺑ ﺎﭘورا د .ا ﯾ ﻦ ﯾ ﻪﺘﻓﺎ ﺎﻫ ﻧ ﯿ زﺎ
ﺎﭘ ﻪﺑ ﯾ ﺶ ﺑ ﯿ ﺮﺘﺸ ﮔدﻮﻟآ ﯽ ﺴﮐﻮﺗﻼﻓآ ﯿﻨ
ﯽ ﻌﻤﺟ و ﺪﺠﻨـﺳ ردﻮﭘ ـﯿ
ﺖ ا رد ار نآ هﺪـﻨﻨﮐ فﺮﺼـﻣ ـﯾ
ناﺮ نﺎﺸـﻧ
ﻣ ﯽ ﺪﻫد .
ﻪﻠﺠﻣ
مﻮﻠﻋ و ﺎﻨﺻ ﯾ ﻊ اﺬﻏ ﯽﯾ اﯾ ناﺮ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣﺖﯾﺎﺳ
ﻪﻟﺎﻘﻣ ﻤﻠﻋ ﯽ ﺸﻫوﮋﭘ_ ﯽ
1 - ﻪﻣﺪﻘﻣ
Elaeagnus angustifolia ﯽﺳور نﻮﺘﯾز ناﻮﻨﻋ ﻪﺑ ﻻﻮﻤﻌﻣ ﻪﮐ
ﯽﻣ ﻪﺘﺧﺎﻨﺷ هداﻮﻧﺎﺧ زا ﯽﺘﺧرد هﺎﯿﮔ ،دﻮﺷ
Elaeagnus هدﻮﺑ
1] [
و ﺖﺳا فوﺮﻌﻣ ﺪﺠﻨﺳ مﺎﻧ ﺎﺑ ناﺮﯾا رد .
ياراد ﯽﮐارﻮـﺧ هﻮـﯿﻣ ﻦـﯾا
هﻮﻬﻗ و ﮏﺸﺧ ﯽﺘﻓﺎﺑ ﯽـﻣ و هدﻮﺑ ﮓﻧر يا
زا ﯽﻌﯿـﺳو ﻒـﯿﻃ ﺪـﻧاﻮﺗ
ﺪﯾﺎﻤﻧ ﻞﻤﺤﺗ ار ﯽﻄﯿﺤﻣ ﻂﯾاﺮﺷ 2]
.[ ﻪﯾﺬﻐﺗ شزرا زا ﺪﺠﻨﺳ هﻮﯿﻣ يا
ﻦﯿﻣﺎـﺘﯾو ،ﻦﯿﺌﺗوﺮﭘ ،تارﺪﯿﻫﻮﺑﺮﮐ يوﺎﺣ و هدﻮﺑ رادرﻮﺧﺮﺑ ﻻﺎﺑ يﺎـﻫ
،A
،B وC ﯽﻣ K ﺪﺷﺎﺑ . داﻮـﻣ ﻦﯾﺮـﺘﻧاواﺮﻓ ﺮﻔﺴﻓ و ﻢﯾﺪﺳ ،ﻢﯿﺳﺎﺘﭘ
ﯾا ﯽﻧﺪﻌﻣ هدﻮﺑ هﻮﯿﻣ ﻦ 3]
،لوﺮﺘـﺳاﻮﺘﯿﺳ ،يﺪـﯿﺋﻮﻧوﻼﻓ تﺎـﺒﯿﮐﺮﺗ و [
ﻦﻧﺎﺗ و ﺪﯿﺋﻮﻨﺗورﺎﮐ ،ﻦﯿﻧﻮﭘﺎﺳ ،ﻦﯾرﺎﻣﻮﮐ ،ﺪﯿﺋﻮﻨﭘﺮﺗ دﻮﺟو نآ رد ﻢﻫ ﺎﻫ
ﺪﻧراد 4] . [ ﯽﺑﺎـﻬﺘﻟا ﺪﺿ ،ﯽﺑوﺮﮑﯿﻣ ﺪﺿ ،ﯽﻧاﺪﯿﺴﮐا ﯽﺘﻧآ صاﻮﺧ 5]
[
ﯽﻧﺎﻃﺮﺳ ﺪﺿ و 6]
هﻮـﯿﻣ ،ﯽﺘﻨـﺳ ﺐـﻃ رد و هﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﺪﺠﻨﺳ[
رﺎــﻤﯿﺑ رد درد ﺪــﺿ ﻞــﻣﺎﻋ ﮏــﯾ ناﻮــﻨﻋ ﻪــﺑ ﺪﺠﻨــﺳ ﻪــﺑ ﻼﺘــﺒﻣ نا
ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ ﺖﯾﺮﺗرآﻮﺌﺘﺳا 7]
.[ ﺮﯿﺛﺎﺗ زا ﯽﺗﺎﺷراﺰﮔ
ﻢـﺳآ و لﺎﻬـﺳا ،نﺎـﻗﺮﯾ ،غاﺮﻔﺘـﺳا ،عﻮـﻬﺗ نﺎﻣرد رد هﻮﯿﻣ ﻦﯾا ﺪﯿﻔﻣ دراد دﻮﺟو 8]
هﺎﯿﮔ ﻦﯾا ﯽﻧﺎﻣرد صاﻮﺧ زا ﯽﮐﺎﺣ ﺮﯿﺧا تﺎﻘﯿﻘﺤﺗ و[
ﯽﻣ هﺪﯾد ﺐﯿﺳآ دﺮﻓ رد ﻢﺧز دﻮﺒﻬﺑ نﺎﻣز ﺶﻫﺎﮐ رد ﺪﺷﺎﺑ
9] .[
د ﺖﻓﺮﺸﯿﭘ ﻢﻏﺮﯿﻠﻋ آ ،يژﻮﻟﻮﻨﮑﺗ ر
ﻮﻟ د ﺎﻫﯽﮔ ي ﺎﻗ ر ﻮﻣ ﯽﭼ و ﯽﯾاﺬﻏ دا
ا ﺮﺛ تا ﯽﺷﺎﻧ زا ﺮﺼﻣ آ داﻮﻣ ﻦﯾا ف ﻮﻟ
هد ﺮﺘﺴﮔ ﺖﺳا ﻪﺘﻓﺎﯾ ش 10]
. [
ﺎﻗ چر ﺎﻫ ر لﻮﻃ رد ﻮﺧ ﺪﺷ ،د ﻼﻋ هو ﺶﻫﺎﮐ ﺮﺑ شزرا ﺬﻏ ا ﯽﯾ ،ﺎﻫاﺬﻏ
ﺖﯿﻟﻮﺑﺎﺘﻣ يﺎﻫ ﻪﯾﻮﻧﺎﺛ يا ﺎﻧ ﻪﺑ م ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ ﺎﻫ
زا ار ﻮﺧ د ﺎﺟﺮﺑ ي
ﺬﮔﯽﻣ را ﻪﮐ ﺪﻧ ضراﻮﻋ ﺪﯾﺪﺷ نﻮﭼ ي ﺰﻧﺎﻃﺮﺳ ا ﯽﯾ رد ار ﻣ ﻮﺟﻮ تاد
ﺪﻨﮐ ﯽﻣ دﺎﺠﯾا هﺪﻧز 11]
.[ ﻪﺑ ﯽﮔدﻮﻟآ داﺪﻌﺘﺳا ،ﯽﯾاﺬﻏ داﻮﻣ مﺎﻤﺗ
ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ چرﺎﻗ و ﺎﻫ
ﺪﻧراد ار ﺎﻬﻧآ ﺪﻟﻮﻣ يﺎﻫ 12]
.[ نﺎﯿﻣ رد 400
ﻦﯿﺴﮐﻮﺗﻼﻓآ ،هﺪﺷ ﯽﯾﺎﺳﺎﻨﺷ ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ عﻮﻧ يﺎﻫ
B1
، B2
، G1
و
G2
ﺪﻧراد يﺮﺘﺸﯿﺑ ﺖﯿﻤﻫا .
ﻦﯿﺴﮐﻮﺗﻼﻓآ ،ﻦﯿﺑ ﻦﯾا رد B1
ناﻮﻨﻋ ﻪﺑ
يﻮﻗ ﺳ هدﺎﻣ ﻦﯾﺮﺗ نﺎﻃﺮ
ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷ از 13]
.[ ،ﺮﺿﺎﺣ لﺎﺣ رد
ﯽﮔدﻮﻟآ ﺮﺑ ﯽﻨﺒﻣ ﯽﺗﺎﺷراﺰﮔ ﺞﻧﺮﺑ
ا ،ترذ ، ﻮﻧ عا ﺎﻫﺰﻐﻣ ي ﻮﺧ ار ﯽﮐ
ﻪﺘﺴﭘ ﻞﺜﻣ ، ﺪﻨﻓ و ق ﺑـ ﺎ ز ماد ﻨﯿﻣ ـ ﯽ ، ﻔﻠﻓ ـ ﻞ ودا و ﯾـ ﺟﻪ ـﺎ ﺮﯿﺷ ،ت
ماد و نﺎﺴﻧا ﺑـ
ﻪ آ ﻦﯿﺴﮐﻮﺗﻼﻓ ﯽﺳرﺮﺑ مﺎﺠﻧا ﺮﺑ و ﻪﺘﺷاد دﻮﺟو
يﺎﻫ
ﯽﻣ ﺪﯿﮐﺎﺗ ﯽﯾاﺬﻏ داﻮﻣ ﻪﻤﻫ رد ﺮﺘﺸﯿﺑ دﻮﺷ
14] . [
ﺮﻫ ﻪﮐ ﺪﻨﭼ فﺮﺼﻣ ﻪﻧاﺮﺳ درﻮﻣ رد ﯽﻤﺳر رﺎﻣآ ﺪﺠﻨﺳ
ناﺮﯾا رد
ﯽﻣ ﺮﻈﻧ ﻪﺑ ﯽﻟو دراﺪﻧ دﻮﺟو ﺪﺳر
لﺎﺳ رد شزرا ﻞﯿﻟد ﻪﺑ ،ﺮﯿﺧا يﺎﻫ
ﻪﯾﺬﻐﺗ يا ﯽﯾاﺬﻏ هدﺎﻣ ﻦﯾا 15]
،[ و ﻞﻣﺎﮐ هﻮﯿﻣ ترﻮﺻ ﻪﺑ نآ فﺮﺼﻣ
ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﯽﻣﻮﻤﻋ لﺎﺒﻗا درﻮﻣ ردﻮﭘ ﺎﯾ .ﺑ
ـ ﺟﻮﺗ ﺎ ـ ﺑ ﻪ ـ ﻪ آﺛ ـﺎ ر
ﻤﻬﻣ ـ ﮐ ﯽ ـ ﻪ آ ﺎﻫﻦﯿﺴﮐﻮﺗﻼﻓ ﺮﺑ
ﺳ ـ ﻣﻼ ﯽﺘ اﻧـ ﺎﺴ راﺮـﻗ و ﺪﻧراد ن ياﺮـﺑ يﺪـﺟ ﺮـﻄﺧ ﮏـﯾ ،ﻦﯿﺴـﮐﻮﺗﻼﻓآ ضﺮـﻌﻣ رد ﻦﺘﻓﺮـﮔ
ﯽـﻣ بﻮﺴﺤﻣ ﯽﯾاﺬﻏ داﻮﻣ ﺖﺷاﺪﻬﺑ و ﺖﻣﻼـﺳ ﯽﺳرﺮﺑ ،ددﺮـﮔ
ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ فﺪﻫ ﺪﺠﻨﺳ ردﻮﭘ رد ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ لﺎﻤﺘﺣا ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ .
2 - شور و داﻮﻣ ﺎﻫ
ﻮﻣ ﻪﺑﺎﺸﻣ ﻪﻌﻟﺎﻄﻣ ﺎﺑ ﻖﺑﺎﻄﻣ ،ﯽﻔﯿﺻﻮﺗ ﻪﻌﻟﺎﻄﻣ ﻦﯾا رد دﻮﺟ
16]
،[
20 ﻪﻧﻮﻤﻧ 100 ﺪﺠﻨﺳ ردﻮﭘ ﯽﻣﺮﮔ )
10 يرﺎﻄﻋ زا ﻪﻧﻮﻤﻧ و ﺎﻫ
10
يرﺎﺒﮑﺸﺧ زا ﻪﻧﻮﻤﻧ ﺎﻫ
( ﯽﻓدﺎﺼﺗ ترﻮﺻ ﻪﺑ -
ﻪﺷﻮﺧ سﺎﺳا ﺮﺑ و يا
ﻞﺤﻣ ﯽﮔﺪﻨﮐاﺮﭘ ﺮﻬﺷ ﯽﯾﺎﯿﻓاﺮﻐﺟ ﻒﻠﺘﺨﻣ ﯽﺣاﻮﻧ زا شوﺮﻓ يﺎﻫ
هﺎﻣ رﻮﯾﺮﻬﺷ رد ﻞﯿﺑدرا 1397
ﻪﺑ ناﺮﯾا ﯽﻠﻣ دراﺪﻧﺎﺘﺳا ﺎﺑ ﻖﺑﺎﻄﻣ و
هرﺎﻤﺷ 13535 17] ﺪﺷ ﻪﯿﻬﺗ [ . ﻤﻧ ﻪﻧﻮ ﻪﺘﺴﺑ رد ﺎﻫ و ﻪﯿﻬﺗ ﯽﻧﻮﻠﯾﺎﻧ يﺎﻫ
نآ ﻪﻧﺎﻫد ﯽﮔدﻮﻟآ زا يﺮﯿﮔﻮﻠﺟ رﻮﻈﻨﻣ ﻪﺑ ﺎﻫ بﺬﺟ و ﯽﻟﺎﻤﺘﺣا يﺎﻫ
ﺪﺷ سﺮﭘ ﺖﺑﻮﻃر .
ﻪﺘﺴﺑ يور ﯽﻧﺎﺸﻧ و ﺦﯾرﺎﺗ ﺮﯿﻈﻧ ﯽﺗﺎﻋﻼﻃا ﺎﻫ
ﻪﻧﻮﻤﻧ ﻞﺤﻣ ﺪﯾدﺮﮔ ﺖﺒﺛ ﻪﻧﻮﻤﻧ ﻢﺠﺣ و يرادﺮﺑ
. ﻪﻧﻮﻤﻧ ﺲﭙﺳ رد ﺎﻫ
فرﺎﻌﺘﻣ يﺎﻣد )
25 داﺮﮔ ﯽﺘﻧﺎﺳ ﻪﺟرد (
ﺎﻣزآ ﻪﺑ لﺎﻘﺘﻧا نﺎﻣز ﺎﺗ هﺎﮕﺸﯾ
ﺪﻧﺪﺷ يراﺪﻬﮕﻧ .
ﺎﺑ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ زا هدﺎﻔﺘﺳا ﺎﺑ ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ ﻦﯿﯿﻌﺗ ﺖﻓﺮﮔ ترﻮﺻ ﯽﺘﯿﻨﯿﻓاﻮﻧﻮﻤﯾا نﻮﺘﺳ ﺎﺑ يزﺎﺳ ﺺﻟﺎﺧ و ﻻﺎﺑ ﯽﯾآرﺎﮐ .
ﺺﻟﺎــﺧ ﻞــﻤﻋ نﻮﺘــﺳ زا هدﺎﻔﺘــﺳا ﺎــﺑ ﯽﯾﺎــﺳﺎﻨﺷ و يزﺎــﺳ
ﯽﺘﯿﻨﯿﻓاﻮﻧﻮﻤﯾا )
ﺖﮐﺮﺷ ﺖﺧﺎﺳ Libios
ﺎﮑﯾﺮﻣآ رﻮﺸﮐ يرﺎﺠﺗ مﺎﻧ ﺎﺑ
Purifast (
هرﺎﻤﺷ ﻪﺑ ناﺮﯾا ﯽﻠﻣ دراﺪﻧﺎﺘﺳا ﺎﺑ ﻖﺑﺎﻄﻣ و 6872
18] [
هﺎﮕﺘﺳد ﮏﻤﮐ ﺎﺑ ﺖﮐﺮﺷ ﺖﺧﺎﺳ HPLC
Waters ﺰﻬﺠﻣ ﺎﮑﯾﺮﻣآ
ﺖﻓﺎﯾ مﺎﺠﻧا ﺲﻧﺎﺳرﻮﻠﻓ زﺎﺳرﺎﮑﺷآ و ﺮﻠﭙﻤﺳﻮﺗا ﻪﺑ .
ﺶﯾﺎﻣزآ ،اﺪﺘﺑا رد هدﻮﻟآ يﺎﻫ
ﻒﻠﺘﺨﻣ حﻮﻄﺳ يﺪﻤﻋ يزﺎﺳ
AFB1
، AFB2
، AFG1
و AFG2
يﺎﻫﺮﺘﻣارﺎﭘ و ﺖﻓﺮﮔ ترﻮﺻ
ﺤﻣ شور ﯽﯾارﺎﮐ ﺪﺷ ﻪﺒﺳﺎ
. ﻪﻧﻮﻤﻧ ﺰﯿﻟﺎﻧآ ،ﺞﯾﺎﺘﻧ لﻮﺼﺣ زا ﺲﭘ يﺎﻫ
ﺪﯾدﺮﮔ زﺎﻏآ ﯽﻠﺻا .
ﻪﻧﻮﻤﻧ جاﺮﺨﺘﺳا ﻪﻠﯿﺳو ﻪﺑ ﺎﻫ
200 ﯽﻠﯿﻣ ﺮﺘﯿﻟ
لﻼﺣ لﻮﻧﺎﺘﻣ 80 ،ﺪﺻرد 20 ناﺰﯿﻣ ﻪﺑ ﮏﻤﻧ و بآ ﺪﺻرد 5
ﺎﺑ مﺮﮔ
تﺪﻣ ﻪﺑ ﺮﮑﯿﺷ زا هدﺎﻔﺘﺳا 5
يور ﺮﺑ ﻪﻘﯿﻗد 50
ﺪﺠﻨﺳ ردﻮﭘ مﺮﮔ
ﺖﻓﺮﮔ ترﻮﺻ .
ﺎﻔﺘﺳا ﺎﺑ قﻮﻓ لﻮﻠﺤﻣ ندﺮﮐ فﺎﺻ زا ﺲﭘ زا هد
هرﺎﻤﺷ ﻦﻤﺗاو يﺎﻫﺬﻏﺎﮐ 4
ﻪﺑ ، 20 ﯽﻠﯿﻣ ،هﺪﺷ فﺎﺻ هرﺎﺼﻋ زا ﺮﺘﯿﻟ
130 هﺪﺷ ﻖﯿﻗر هرﺎﺼﻋ ﺎﺗ ﺪﯾدﺮﮔ ﻪﻓﺎﺿا بآ ﺮﺘﯿﻟ ﯽﻠﯿﻣ 150
ﯽﻠﯿﻣ ﺪﯾآ ﺖﺳد ﻪﺑ يﺮﺘﯿﻟ .
ﻪﻧﻮﻤﻧ يزﺎﺳ ﺺﻟﺎﺧ ياﺮﺑ نﻮﺘﺳ زا ،ﺎﻫ
يوﺎﺣ ﻪﮐ ﯽﺘﯿﻨﯿﻓاﻮﻧﻮﻤﯾا يﺎﻫ
مﻮﻤﺳ ﻪﯿﻠﻋ ﺮﺑ يدﺎﺑ ﯽﺘﻧآ AFB1
، AFB2
، AFG1
و AFG2
ﯽﻣ ﺘﺳا ،ﺪﺷﺎﺑ ﺪﺷ هدﺎﻔ . اﺪﺘﺑا ،رﻮﻈﻨﻣ ﻦﯾا ﻪﺑ 70
ﯽﻠﯿﻣ لﻮﻠﺤﻣ زا ﺮﺘﯿﻟ
ﺮﯿﻄﻘﺗ رﺎﺑود بآ ﺎﺑ نﻮﺘﺳ ﺲﭙﺳ و ﺪﺷ هداد رﻮﺒﻋ نﻮﺘﺳ زا قﻮﻓ ﺪﺷ هداد ﻮﺸﺘﺴﺷ .
مﻮﻤﺳ ،نﻮﺘﺳ ندﺮﮐ ﮏﺸﺧ زا ﺪﻌﺑ
AFB1
، AFB2
، AFG1
و AFG2
ﻂﺳﻮﺗ 1500 ﺮﺘﯿﻟوﺮﮑﯿﻣ
ﺎﺑ يزﺎﺳ ﻖﯿﻗر زا ﺲﭘ و هﺪﯾدﺮﮔ اﺪﺟ ﯽﺘﯿﻨﯿﻓاﻮﻧﻮﻤﯾا نﻮﺘﺳ زا لﻮﻧﺎﺘﻣ 1500 ،ﺮﻄﻘﻣ بآ ﺮﺘﯿﻟوﺮﮑﯿﻣ 100
هﺎﮕﺘﺳد ﻪﺑ ﺮﺘﯿﻟوﺮﮑﯿﻣ
ﺪﺷ ﻖﯾرﺰﺗ ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ .
Fig 1 HPLC chromatogram of aflatoxin standards B1, B2, G1, and G2
،ﻊﯾﺎﻣ ﯽﻓاﺮﮔﻮﺗﺎﻣوﺮﮐ كﺮﺤﺘﻣ زﺎﻓ لﻼﺣ ﻪﯿﻬﺗ رﻮﻈﻨﻣ ﻪﺑ 6
ﻢﺠﺣ
،بآ 2 و ﻞﯾﺮﺘﯿﻧﻮﺘﺳا ﻢﺠﺣ 3
ﺪﺷ طﻮﻠﺨﻣ ﺮﮕﯾﺪﮑﯾ ﺎﺑ لﻮﻧﺎﺘﻣ ﻢﺠﺣ
،كﺮﺤﺘﻣ زﺎﻓ ﺮﺘﯿﻟ ﺮﻫ يازا ﻪﺑ و 350
ﮏﯾﺮﺘﯿﻧ ﺪﯿﺳا ﺮﺘﯿﻟوﺮﮑﯿﻣ 4
و رﻻﻮﻣ 120 مﺮﮕﯿﻠﯿﻣ ﺪﺷ هدﺎﻔﺘﺳا ﻢﯿﺳﺎﺘﭘ ﺪﯿﻣوﺮﺑ .
جﻮﻣ لﻮﻃ
ﺐﯿﺗﺮﺗ ﻪﺑ هدﺎﻔﺘﺳا درﻮﻣ ﺮﺸﻧ و ﯽﮑﯾﺮﺤﺗ 435
و 365 دﻮﺑ ﺮﺘﻣﻮﻧﺎﻧ .
كﺮﺤﺘﻣ زﺎﻓ ﺖﻋﺮﺳ 1
ﯽﻠﯿﻣ ﻖﯾرﺰﺗ ﻢﺠﺣ و ﻪﻘﯿﻗد رد ﺮﺘﯿﻟ 100
ﺪﯾدﺮﮔ بﺎﺨﺘﻧا ﺮﺘﯿﻟوﺮﮑﯿﻣ .
ﻪﺘﻓر رﺎﮐ ﻪﺑ نﻮﺘﺳ C18
) 4.6mm×250mm×5µm; Waters Corp (
دﻮﺑ . ﺖﯾﺎﻬﻧ رد
ﺳاﺮﺒﯿﻟﺎﮐ ﯽﻨﺤﻨﻣ ﻢﺳر زا ﺰﯿﻟﺎﻧآ ﺖﻬﺟ ﯽﺟرﺎﺧ دراﺪﻧﺎﺘﺳا شور و نﻮﯿ
ﺪﺷ هدﺎﻔﺘﺳا ﻦﯿﺴﮐﻮﺗﻼﻓآ .
ياﺮﺑ يرادزﺎﺑ نﺎﻣز AFB1
، AFB2
،
AFG1
و AFG2
ﺐﯿﺗﺮﺗ ﻪﺑ 827
/6 ، 591 /5 ، 619 / 4 و 891 /3
دﻮﺑ ﻪﻘﯿﻗد . ماﺮﮔﻮﺗﺎﻣوﺮﮐ زا ﻪﻧﻮﻤﻧ ﮏﯾ هرﺎﻤﺷ ﻞﮑﺷ رد HPLC
ﺖﺳا هﺪﺷ ﻪﺋارا ﮏﯾ .
ياﺮﺑ ﺖﻓﺎﯾزﺎﺑ ﺪﺻرد و ﯽﮕﺘﺴﺒﻤﻫ ﺐﯾﺮﺿ
ﻦﯿﺴﮐﻮﺗﻼﻓآ B1
ﺐﯿﺗﺮﺗ ﻪﺑ 9987 /0 و 7/
79 % ﺪﻣآ ﺖﺳد ﻪﺑ .
ﺮﯾدﺎﻘﻣ
ياﺮﺑ ﯽﮕﺘﺴﺒﻤﻫ ﺐﯾﺮﺿ AFB2
، AFG1
و AFG2
ﺐﯿﺗﺮﺗ ﻪﺑ
9989 /0 و 9992 /0 و 9998 /0 ﺪﺷ ﻦﯿﯿﻌﺗ .
ﺪﺻرد ﻦﯿﻨﭽﻤﻫ ﻦﯿﺴﮐﻮﺗﻼﻓآ ياﺮﺑ ﺖﻓﺎﯾزﺎﺑ
ﻪﺑ قﻮﻓ يﺎﻫ ﺐﯿﺗﺮﺗ
2/
69 % ، 2/
72 % و
4/
65 % دﻮﺑ يدﺎﻬﻨﺸﯿﭘ هدوﺪﺤﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻪﮐ AOAC
19] [
ﺎﮐ ﻪﺑ شور ﺖﺤﺻ ﯽﻣ ﺪﯿﯾﺎﺗ ﻪﺘﻓر ر
دﻮﺷ .
ود ﻦﯿﮕﻧﺎﯿﻣ ،ﻪﻌﻟﺎﻄﻣ ﻦﯾا رد هزاﺪﻧا رﺎﺑ
ﻦﯿﺴﮐﻮﺗﻼﻓآ يﺮﯿﮔ ﻪﺑ ،ﺎﻫ
ﺖﺳا هﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﻪﻧﻮﻤﻧ ﺮﻫ رد ﯽﯾﺎﻬﻧ هداد ناﻮﻨﻋ .
ياﺮﺑ
هداد ﻊﯾزﻮﺗ ندﻮﺑ لﺎﻣﺮﻧ اﺪﺘﺑا رد ،ﻞﯿﻠﺤﺗ دﺎﻔﺘﺳا ﺎﺑ ﺎﻫ
نﻮﻣزآ زا ه
ﺪﺷ ﯽﺳرﺮﺑ ﻒﻧﺮﯿﻤﺳا فوﺮﮔﻮﻤﻟﻮﮐ .
يﺎﻫﺮﯿﻐﺘﻣ مﺎﻤﺗ ﻪﮐ ﯽﯾﺎﺠﻧآ زا
ياﺮﺑ ﮏﯾﺮﺘﻣارﺎﭘ يﺎﻬﻧﻮﻣزآ زا ،ﺪﻧدﻮﺑ لﺎﻣﺮﻧ ﻊﯾزﻮﺗ ياراد ﯽﻤﮐ هداد ﻞﯿﻠﺤﺗ ﺪﺷ هدﺎﻔﺘﺳا ﺎﻫ
. ﻦﯿﺴﮐﻮﺗﻼﻓآ ﺖﻈﻠﻏ ﻦﯿﮕﻧﺎﯿﻣ ﻪﺴﯾﺎﻘﻣ ﺎﺑ ﺎﻫ
نﻮﻣزآ ﻂﺳﻮﺗ دراﺪﻧﺎﺘﺳا ﺮﯾدﺎﻘﻣ One sample T-Test
مﺎﺠﻧا
ﺖﻓﺎﯾ . راﻮﻣ ﯽﻣﺎﻤﺗ رد د
05 / 0
<
ﯽﻨﻌﻣ يرﺎﻣآ ظﺎﺤﻟ زا p ﺮﻈﻧ رد راد
ﺖﺳا هﺪﺷ ﻪﺘﻓﺮﮔ .
3 - ﺚﺤﺑ و ﺞﯾﺎﺘﻧ
ﻦﯿﺴﮐﻮﺗﻼﻓآ ﺖﻈﻠﻏ ﻪﻧﻮﻤﻧ ﮏﯿﮑﻔﺗ ﻪﺑ ﺪﺠﻨﺳ ردﻮﭘ هﺪﻨﻨﮐ هدﻮﻟآ يﺎﻫ
ﺖﺳا هﺪﺷ هداد نﺎﺸﻧ ﮏﯾ هرﺎﻤﺷ لوﺪﺟ رد .
ﺶﯾﺎﻤﻧ ﻪﮐ رﻮﻃ نﺎﻤﻫ
ﻪﻧﻮﻤﻧ رد ﯽﮔدﻮﻟآ ناﺰﯿﻣ ﺖﺳا هداد رﺎﯿﺴﺑ ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ
ﺖﺳﻻﺎﺑ . ﻫﺎﺸﻣ ،ﺮﮕﯾد فﺮﻃ زا ﯽﻣ هﺪ
ﺖﻈﻠﻏ ﺮﯾدﺎﻘﻣ ﻦﯾﺮﺘﺸﯿﺑ دﻮﺷ
ﻦﯿﺴﮐﻮﺗﻼﻓآ ﻪﺑ طﻮﺑﺮﻣ B1
ﯽﻣ ﺪﺷﺎﺑ . رد ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ
ﻪﻧﻮﻤﻧ ﺖﺳا هﺪﺷ ﻪﺋارا ود هرﺎﻤﺷ لوﺪﺟ رد ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ .
ﺎﻨﺑ
هﺪﻣآ ﺖﺳد ﻪﺑ ﺞﯾﺎﺘﻧ ﺮﺑ 85
ﻪﻧﻮﻤﻧ زا ﺪﺻرد ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ
ﻂﻘﻓ و ﺪﻨﺘﺷاد ار ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ زا عﻮﻧ ﮏﯾ ﻞﻗاﺪﺣ 3
ﻪﻧﻮﻤﻧ
رﻮﻣ دﻮﺑ ﯽﮔدﻮﻟآ زا يرﺎﻋ ﯽﺳرﺮﺑ د .
طﻮﺑﺮﻣ ﯽﮔدﻮﻟآ ﺪﺻرد ﻦﯾﺮﺘﺸﯿﺑ
ﻪﺑ AFB1
و AFB2
ﻪﺑ طﻮﺑﺮﻣ ﯽﮔدﻮﻟآ ﺪﺻرد ﻦﯾﺮﺘﻤﮐ و AFG2
ﺪﯾدﺮﮔ شراﺰﮔ يﺎﻫدراﺪﻧﺎﺘﺳا ﺎﺑ هﺪﻣآ ﺖﺳد ﻪﺑ ﺞﯾﺎﺘﻧ ﻪﺴﯾﺎﻘﻣ .
ناﺮﯾا ) 20 ( ﺎﭘورا ﻪﯾدﺎﺤﺗا و )
21 ( ﻦﯿﺴﮐﻮﺗﻼﻓآ ﻪﻔﻟﻮﻣ ود سﺎﺳا ﺮﺑ
و مﺎﺗ AFB1
ﻪﮐ ﻪﯿﺻﻮﺗ ﻮﺻ ﺎﻬﻧآ سﺎﺳا ﺮﺑ ﺎﻫ ﯽﻣ تر
،دﺮﯿﮔ رد
ﺖﺳا هﺪﺷ ﻪﺋارا ﻪﺳ هرﺎﻤﺷ لوﺪﺟ .
ﻪﺘﻓﺎﯾ ﻪﺑ ﺎﻨﺑ ﻦﯿﮕﻧﺎﯿﻣ ،ﺎﻫ
ﻦﯿﺴﮐﻮﺗﻼﻓآ و مﺎﺗ ﻦﯿﺴﮐﻮﺗﻼﻓآ B1
هﺪﺷ ﯽﺳرﺮﺑ ﺪﺠﻨﺳ يﺎﻫردﻮﭘ
ﻦﯾا زا ﺮﺗﻻﺎﺑ رﺎﯿﺴﺑ و ﺖﺷاد دراﺪﻧﺎﺘﺳا ﺮﯾدﺎﻘﻣ ﺎﺑ يراد ﯽﻨﻌﻣ توﺎﻔﺗ دﻮﺑ ﺮﯾدﺎﻘﻣ .
ﻼﻓآ ﻦﯿﺴﮐﻮﺗ ﺎﻫ ﯽﮑﯾ زا ﻦﯾﺮﺘﻤﻬﻣ آ ﺪﻨﯾﻻ ه ﺎﻫ ي ﻮﻣ دا ﺬﻏ ا ﮐ ﺪﻨﺘﺴﻫ ﯽﯾ ﻪ
ﯽﻣ ﻮﺗ ا ﺐﺒﺳ ﺪﻨﻧ ا
ﺮﺛ تا ﯽﻔﻠﺘﺨﻣ ا يور ﺎﺴﻧ ﺪﻧﻮﺷ ن 22] .[ ﯽﺳرﺮﺑ
ﯽﻣ نﺎﺸﻧ ﻊﺑﺎﻨﻣ هزاﺪﻧا درﻮﻣ رد ﯽﺷراﺰﮔ ﭻﯿﻫ نﻮﻨﮐ ﺎﺗ ﺪﻫد
يﺮﯿﮔ
دراﺪﻧ دﻮﺟو ﺪﺠﻨﺳ ردﻮﭘ رد ﻦﯿﺴﮐﻮﺗﻼﻓآ .
ﻪﻌﻟﺎﻄﻣ رد ،ور ﻦﯾا زا
رد ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ ناﺰﯿﻣ ﯽﺳرﺮﺑ ﻪﺑ رﺎﺑ ﻦﯿﻟوا ياﺮﺑ ﺮﺿﺎﺣ
ﺘﺧادﺮﭘ هﺪﺷ ﯽﺷوﺮﻓ هدﺮﺧ ﺪﺠﻨﺳ ردﻮﭘ ﻪ
ﻢﯾا . داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﺞﯾﺎﺘﻧ
ﺖﺳﻻﺎﺑ رﺎﯿﺴﺑ ﯽﺳرﺮﺑ درﻮﻣ ﺪﺠﻨﺳ يﺎﻫردﻮﭘ رد ﯽﮔدﻮﻟآ ناﺰﯿﻣ ﻪﮐ .
ﻪﮐ ﯽﯾﺎﺠﻧآ زا ﮐﻮﺗﻼﻓآ ﯽﻤﺳ تاﺮﺛا
ـ ﺑ ﻦﯿﺴ ـ ﻪ ناﺰﯿﻣ تﺪﻣ و فﺮﺼﻣ نآ زا هدﺎﻔﺘﺳا نﺎﻣز
ﯽﮕﺘﺴﺑ و دراد ﯽﻣ ﺪﻧاﻮﺗ ﻟﺎﺣ ود ﻪﺑ ـ ﺣ ﺖ ـ و دﺎ
ﻣـ ﻦﻣﺰ هﺪﯾد دﻮﺷ 23] ،[ ﺶﯾﺎﭘ ﻪﺑ زﺎﯿﻧ ﯽﻣ ﺺﺨﺸﻣ ﺮﺘﺸﯿﺑ يﺎﻫ
دﻮﺷ .
Table 1 Aflatoxin concentrations in Elaeagnus angustifolia L. powder Sample AFB1
(µg/kg)
AFB2 (µg/kg)
AFG1 (µg/kg)
AFG2 (µg/kg)
Total Aflatoxin (µg/kg)`
1 0.0 0.0 0.0 0.0 0.0
2 0.0 0.0 0.0 0.0 0.0
3 0.0 0.0 0.0 0.0 0.0
4 23.0 5.2 2.2 0.0 30.4
5 24.4 4.4 6.0 0.0 34.8
6 82.0 18.0 14.0 14.0 128.0
7 92.0 22.0 12.0 6.0 132.0
8 80.0 28.0 14.0 12.0 134.0
9 98.0 20.0 20.0 8.0 146.0
10 114.0 20.0 26.0 0.0 160.0
11 156.0 26.0 16.0 10.0 208.0
12 234.0 40.0 16.0 14.0 304.0
13 330.0 60.0 60.0 100.0 550.0
14 450.0 80.0 0.0 100.0 630.0
15 430.0 90.0 70.0 130.0 720.0
16 720.0 100.0 80.0 100.0 1000.0
17 890.0 100.0 60.0 50.0 1100.0
18 1320.0 160.0 90.0 90.0 1660.0 19 1470.0 170.0 160.0 60.0 1860.0 20 1900.0 220.0 80.0 160.0 2360.0
Table 2Aflatoxin contamination in Elaeagnus angustifolia L. powder
Variable Positive samples Positive samples
% Mean (μg/kg) Maximum
(μg/kg)
AFB1 17 85 420.67 1900.0
AFB2 17 85 58.18 220.0
AFG1 16 80 36.31 160.0
AFG2 14 70 42.70 160.0
Total Aflatoxin 17 85 557.86 2360.0
Table 3Comparison of total aflatoxin and aflatoxin B1 with Iranian National Standard and European
Commission Standard
P-value*
European Commission Standard (μg/kg) Iranian National
Standard (μg/kg) Mean
(μg/kg) Variable
<0.001 4
15 557.86
Total Aflatoxin
<0.001 2
5 420.67
AFB1
*One sample T-Test
ﯽﻣ هﺪﻫﺎﺸﻣ ﻪﮐ ﻪﻧﻮﮕﻧﺎﻤﻫ ،ﺮﮕﯾد فﺮﻃ زا ﺘﺸﯿﺑ دﻮﺷ
ﺮﯾدﺎﻘﻣ ﻦﯾﺮ
ﻦﯿﺴﮐﻮﺗﻼﻓآ ﻪﺑ طﻮﺑﺮﻣ ﺖﻈﻠﻏ B1
ﯽﻣ ﺪﺷﺎﺑ . ﻪﺑ ﻦﯿﺴﮐﻮﺗﻼﻓآ ﻦﯾا
ﯽﻤﺳ ناﻮﻨﻋ نﺎﻃﺮﺳ و ﻦﯾﺮﺗ
ﻦﯿﺴﮐﻮﺗﻼﻓآ عﻮﻧ ﻦﯾﺮﺗاز ﻪﺘﺧﺎﻨﺷ ﺎﻫ
ﺖﺳا هﺪﺷ 24]
راﺮﻗ نژﻮﻨﯿﺳرﺎﮐ داﻮﻣ لوا ﻪﺘﺳد رد و [
ﯽﻣ دﺮﯿﮔ 25] .[ ﻦﺘﻓﺮﮔ راﺮﻗ ﻪﮐ ﺖﺳا هداد نﺎﺸﻧ ﻦﯾا ﺮﺑ هوﻼﻋ
ﮐﺮﺳ ﻪﺑ ﺮﺠﻨﻣ ﻦﯿﺴﮐﻮﺗﻼﻓآ ضﺮﻌﻣ رد ﻦﻣﺰﻣ و ﯽﻨﻤﯾا ﻢﺘﺴﯿﺳ بﻮ
يﺬﻐﻣ ﺰﯾر ﯽﺧﺮﺑ و ﻦﯿﺌﺗوﺮﭘ ﻢﺴﯿﻟﻮﺑﺎﺘﻣ رد ﻞﺧاﺪﺗ ﺎﻫ
ﯽﻣ دﻮﺷ 26] . [ دوﺪﺣ ﻪﮐ ﺖﺳا هﺪﺷ هدز ﻦﯿﻤﺨﺗ 5/
4 ﺮﻔﻧ درﺎﯿﻠﯿﻣ
ﻦﯿﺴﮐﻮﺗﻼﻓآ ضﺮﻌﻣ رد ﻪﻌﺳﻮﺗ لﺎﺣ رد يﺎﻫرﻮﺸﮐ رد لﺮﺘﻨﮐ يﺎﻫ
ﺪﻨﺷﺎﺑ ﻪﺘﺷاد راﺮﻗ هﺪﺸﻧ 27]
.[ يوﺎﺣ يﺎﻫاﺬﻏ فﺮﺼﻣ ﻦﯿﺑ ﻦﯾا رد
ﮑﯿﺴﮐﻮﺗﻼﻓآ ﻞﻣﺎﻋ هﺪﻤﻋ ﻦﯿﺴﮐﻮﺗﻼﻓآ ﯽﻣ ﻪﺘﺧﺎﻨﺷ ﺲﯾزﻮ
دﻮﺷ 1] . [
ناﺰﯿﻣ ﻪﺑ ﻦﯿﺴﮐﻮﺗﻼﻓآ ضﺮﻌﻣ رد ﻦﺘﻓﺮﮔ راﺮﻗ لﺎﺴﮔرﺰﺑ داﺮﻓا رد زا ﺶﯿﺑ 20 تﺪﻣ رد نﺪﺑ نزو مﺮﮔﻮﻠﯿﮐ ﺐﺴﺣ ﺮﺑ مﺮﮔوﺮﮑﯿﻣ
نﺎﻣز 21 - 7 ﯽﻣ ﯽﺘﺣ زور ددﺮﮔ گﺮﻣ ﻪﺑ ﺮﺠﻨﻣ ﺪﻧاﻮﺗ
28] . [ رد
لﺎﺳ رد گﺮﻣ ﻪﺑ ﺮﺠﻨﻣ ﺪﯾﺪﺷ ﺲﯾزﻮﮑﯿﺴﮐﻮﺗﻼﻓآ ﻪﺘﺷﺬﮔ يﺎﻫ
ﺪﺷ شراﺰﮔ ﺪﻨﻫ و ﺎﯿﻨﮐ يﺎﻫرﻮﺸﮐ ﺖﺳا ه
29] . [ ﻪﺟﻮﺗ ﯽﺘﺴﯾﺎﺑ
ﻦﯿﺴﮐﻮﺗﻮﮑﯾﺎﻣ ﺐﻠﻏا ﻪﮐ ﯽﯾﺎﺠﻧآ زا ﻪﮐ ﺖﺷاد ﯽﻨﯿﺌﺗوﺮﭘ رﺎﺘﺧﺎﺳ ﺎﻫ
ﯽﻤﻧ ﯽﯾﺎﺳﺎﻨﺷ ماد و نﺎﺴﻧا ﻂﺳﻮﺗ ،ﺪﻧراﺪﻧ رﺎﺛآ وﺮﻨﯾا زا و ﺪﻧﻮﺷ
ﯽﻣ ﺮﻫﺎﻇ ﯽﻧﺎﻣز مﻮﻤﺳ ﻦﯾا ﺖﻓﺎﯾرد ﻼﻣﺎﮐ يرﺎﻤﯿﺑ ﻪﮐ دﻮﺷ
ﺖﺳا هدﺮﮐ ﺖﻓﺮﺸﯿﭘ 30]
.[ ﺪﻨﭼ ﻪﺑ نﺎﻣﺰﻤﻫ ﯽﮔدﻮﻟآ ﻦﯿﻨﭽﻤﻫ
ﻞﯿﻟد ﻪﺑ ،ﻦﯿﺴﮐﻮﺗﻼﻓآ ﻢﻫ تاﺮﺛا
ﯽﻣ ﯽﮔﺪﻨﻨﮐ ﺪﯾﺪﺸﺗ و ﯽﯾاﺰﻓا ﺪﻧاﻮﺗ
يﺪﺟ ضراﻮﻋ ﺪﯾﺎﻤﻧ دﺎﺠﯾا هﺪﻨﻨﮐ فﺮﺼﻣ ياﺮﺑ يﺮﺗ
22] .[ ﻦﯾا
ﯽﮔدﻮﻟآ ﯽﺳرﺮﺑ ﺮﺑ دﺪﺠﻣ يﺪﯿﮐﺎﺗ دﻮﺧ دراﻮﻣ داﻮﻣ ﯽﻟﺎﻤﺘﺣا يﺎﻫ
ﺪﻨﺘﺴﻫ ﺎﻬﻧآ لﺮﺘﻨﮐ موﺰﻟ ترﻮﺻ رد و ﯽﻓﺮﺼﻣ ﯽﯾاﺬﻏ .
ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ ياﺮﺑ ﯽﻟﺎﻤﺘﺣا ﻞﯾﻻد ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ رد رﺮﺑ درﻮﻣ ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ ﯽﺳ .
و ﺎﻣد ،رﻮﻧ ﺮﯿﻈﻧ ﯽﻄﯿﺤﻣ ﻂﯾاﺮﺷ
ﻪﺘﺧﺎﻨﺷ ﻦﯿﺴﮐﻮﺗﻼﻓآ ﺪﯿﻟﻮﺗ ﺮﺑ ﺮﺛﻮﻣ ﻢﻬﻣ ﻞﻣاﻮﻋ زا pH
هﺪﺷ ﺪﻧا 31] .[ نﺎﻣز و هﺎﻣ ﺮﻬﻣ ،ﺪﺠﻨﺳ هزﺎﺗ هﻮﯿﻣ ﺖﺷادﺮﺑ نﺎﻣز
ﻪﻧﻮﻤﻧ ﺖﺳا هدﻮﺑ هﺎﻣ رﻮﯾﺮﻬﺷ ،ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ يرادﺮﺑ .
ﻪﺑ ﻦﯾاﺮﺑﺎﻨﺑ
ﻪﻧﻮﻤﻧ ﺪﺳﺮﯿﻣ ﺮﻈﻧ هدﻮﺑ ﺶﯿﭘ لﺎﺳ ﺪﯿﻟﻮﺗ ﻪﺑ طﻮﺑﺮﻣ ﺎﻫ
ﺪﻨﺷﺎﺑ . رﺎﻈﺘﻧا
ﯽﻣ ﻪﻠﻓ ترﻮﺻ ﻪﺑ ﺪﺠﻨﺳ ردﻮﭘ شوﺮﻓ دور دراﻮﻣ ﺐﻠﻏا رد و يا
ﻪﺘﺴﺑ نوﺪﺑ هدﺎﻣ ﻦﯾا ﺖﺑﻮﻃر ﺶﯾاﺰﻓا لﺎﻤﺘﺣا نﺎﻣز لﻮﻃ رد ،يﺪﻨﺑ
دﺮﺒﺑ ﻻﺎﺑ ار ﯽﯾاﺬﻏ .
دﻮﺟو ﺮﺑ هوﻼﻋ ﻪﮐ ﺖﺳا هﺪﺷ هداد نﺎﺸﻧ
چرﺎﻗ ﺪﺷر لﺎﻤﺘﺣا ﺰﯿﻧ نﺎﻣز ﺖﺷﺬﮔ ،ﺖﺑﻮﻃر ﺪﻫاﻮﺧ ﻻﺎﺑ ار ﺎﻫ
دﺮﺑ 32] . [ ﻓ ﻪﺘﻔﮔ ﻪﺑ ﺎﻨﺑ رد هﺪﺷ ﻪﺿﺮﻋ لﻮﺼﺤﻣ ،نﺎﮔﺪﻨﺷوﺮ
ﯽﻣ هﻮﯿﻣ ﻞﮐ بﺎﯿﺳآ ،رازﺎﺑ ﺪﺷﺎﺑ
. ﯽﻣ ﻦﯾاﺮﺑﺎﻨﺑ ﻪﮐ ﺖﺷاد رﺎﻈﺘﻧا ناﻮﺗ
ﯽﻨﻌﯾ ﺪﺠﻨﺳ ردﻮﭘ رد دﻮﺟﻮﻣ ﻪﺑﻮﻃﺮﻟا بذﺎﺟ تﺎﺒﯿﮐﺮﺗ ﻞﯿﻟد ﻪﺑ ﻪﮕﻧ ترﺪﻗ و بﺬﺟ ﺎﺑ ﻪﮐ يﺪﻨﻗ تﺎﺒﯿﮐﺮﺗ و ﺮﺒﯿﻓ
بآ ﺮﺘﺸﯿﺑ يراد
ﻪﺘﻓﺎﯾ ﺶﯾاﺰﻓا ﺖﺑﻮﻃر راﺪﻘﻣ ،ﺪﻨﺘﺴﻫ هاﺮﻤﻫ 33]
ﯽﮔدﻮﻟآ ﺢﻄﺳ و[
ﻻﺎﺑ دور ﺮﺗ . ﻞﯿﻟد ﻪﺑ ﻂﻘﻓ ﻦﯿﺴﮐﻮﺗﻼﻓآ ﺪﯿﻟﻮﺗ ﻪﮐ ﺖﺳا ﺺﺨﺸﻣ
ﺖﺷادﺮﺑ زا ﻞﺒﻗ رد ﻪﮑﻠﺑ ،ﺖﺴﯿﻧ ﺐﺳﺎﻨﻣﺎﻧ يراﺪﻬﮕﻧ ﻂﯾاﺮﺷ ﺪﺘﻔﯿﺑ قﺎﻔﺗا ﺖﺳا ﻦﮑﻤﻣ ﯽﮔدﻮﻟآ ﺰﯿﻧ لﻮﺼﺤﻣ .
راﺮﻗ ﻦﯿﻨﭽﻤﻫ
لﺎﻤﺘﺣا ﺰﯿﻧ ،ﯽﻟﺎﺴﮑﺸﺧ نﻮﭼ ﯽﺸﻨﺗ ﻞﻣاﻮﻋ ﺖﺤﺗ هﺎﯿﮔ ﻦﺘﻓﺮﮔ ﯽﻣ ﻻﺎﺑ ﻦﯿﺴﮐﻮﺗﻼﻓآ ﺪﯿﻟﻮﺗ دﺮﺑ
34] .[
ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ا
ﺮﺛ و تا ﻮﻋ ضرا ﺮﻀﻣ ا ﻮﻤﺳ ﻦﯾ رد م ﻮﻣ دا ﺬﻏ ا ﯽﯾ ،
ﺎﺳ ز ﺎﻣ ن ﺎﻫ ي ﻦﯿﺑ ا ﯽﻠﻠﻤﻟ و ﺪﺣ ﯽﻠﻣ ار يزﺎﺠﻣ ﺮﺑ
نآ يا ﺎﻫ رد
ﻮﻣ ﯽﺧﺮﺑ دا ﺬﻏ ا ﯽﯾ هدﺮﮐ مﻼﻋا ﺪﻧا
. ﻦﮑﻤﻣ دوﺪﺣ ﻦﯾا ﻪﮐ ﺪﻨﭼ ﺮﻫ
ﺪﺷﺎﺑ هدﻮﺑ توﺎﻔﺘﻣ ﺎﻫرﻮﺸﮐ ﺐﺴﺣ ﺮﺑ ﺖﺳا 11]
. [ ﻪﻌﻟﺎﻄﻣ رد
ﻦﯿﺴﮐﻮﺗﻼﻓآ و مﺎﺗ ﻦﯿﺴﮐﻮﺗﻼﻓآ ﻦﯿﮕﻧﺎﯿﻣ ،ﺮﺿﺎﺣ B1
يﺎﻫردﻮﭘ
ناﺮﯾا دراﺪﻧﺎﺘﺳا ﺮﯾدﺎﻘﻣ زا ﺮﺗﻻﺎﺑ رﺎﯿﺴﺑ هﺪﺷ ﯽﺳرﺮﺑ ﺪﺠﻨﺳ 20]
[
ﺎﭘورا ﻪﯾدﺎﺤﺗا و 21]
دﻮﺑ[ . ﺪﺠﻨﺳ ردﻮﭘ فﺮﺼﻣ ﻪﻧاﺮﺳ ﻪﮐ ﺪﻨﭼ ﺮﻫ
ﻦﺘﻓﺮـﮔ راﺮـﻗ ﻪﮐ ﯽﯾﺎﺠﻧآ زا ﯽﻟو ،ﺖﺴﯿﻧ ﺺﺨﺸﻣ مدﺮﻣ ﻦﯿﺑ رد بﻮﺴﺤﻣ يﺪـﺟ ﺮـﻄﺧ ﮏـﯾ ،ﻦﯿﺴـﮐﻮﺗﻼﻓآ ضﺮـﻌﻣ رد
ﯽـﻣ رد نآ ﺐﺳﺎﻨﻣ لﺮﺘﻨﮐ ﻦﯾاﺮﺑﺎﻨﺑ ،ددﺮـﮔ ﯽﻓﺮﺼﻣ ﯽﯾاﺬﻏ مﻼﻗا
دﻮﺑ ﺪﻫاﻮﺧ ﺖﯾﻮﻟوا وﺰﺟ .
ﺖﺷاد هاﺮﻤﻫ ﻪﺑ دﻮﺧ ﺎﺑ ار ﯽﻔﻌﺿ و تﻮﻗ طﺎﻘﻧ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ .
ﻪﻌﻟﺎﻄﻣ ﻦﯿﻟوا ناﻮﻨﻋ ﻪﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﯽﺳرﺮﺑ ﻪﺑ ﻪﮐ ﺖﺳا يا
ناﺮﯾا رد هﺪﺷ ﯽﺷوﺮﻓ هدﺮﺧ ﺪﺠﻨﺳ ردﻮﭘ ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ ﺖﺳا ﻪﺘﺧادﺮﭘ .
ﻪﻧﻮﻤﻧ و ﻪﻧﻮﻤﻧ ﻢﮐ داﺪﻌﺗ زا ﯽﻠﺼﻓ يرادﺮﺑ
دوﺪﺤﻣ ﺖﯾ ﯽﻣ بﻮﺴﺤﻣ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ يﺎﻫ دﻮﺷ
. ﻪﺋارا ياﺮﺑ
ﻖﯿﻗد ﯽﺑﺎﯾزرا ﯽﻣ دﺎﻬﻨﺸﯿﭘ ﺮﺗ
ﻪﻧﻮﻤﻧ داﺪﻌﺗ دﻮﺷ رد و ﺮﺘﺸﯿﺑ يﺎﻫ
ﯽﻧﻻﻮﻃ ﯽﻧﺎﻣز ﻊﻃﺎﻘﻣ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ﺮﺘﺸﯿﺑ ﻞﻣاﻮﻋ ﺎﺑ و ﺮﺗ
ﺪﻧﺮﯿﮔ .
4 - ﻪﺠﯿﺘﻧ يﺮﯿﮔ
ﺪﺠﻨﺳ يﺎﻫردﻮﭘ ندﻮﺑ ﺐﺳﺎﻨﻣﺎﻧ ﺮﮕﻧﺎﺸﻧ ﻞﺻﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺞﯾﺎﺘﻧ ﻬﺷ ﯽﺷوﺮﻓ هدﺮﺧ ﺢﻄﺳ رد هﺪﺷ ﻪﺿﺮﻋ فﺮﺼﻣ ياﺮﺑ ﻞﯿﺑدرا ﺮ
ﯽﻣ ﺪﺷﺎﺑ . ﻪﺘﻓﺎﯾ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺖﻣﻼﺳ ﻆﻔﺣ رﻮﻈﻨﻣ ﻪﺑ و ﺮﺿﺎﺣ يﺎﻫ
ﯽﻣ رﺎﻈﺘﻧا نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ ﺖﺤﺗ تﻻﻮﺼﺤﻣ ﻪﻧﻮﮔ ﻦﯾا ﻪﮐ دور
شزرا ظﺎﺤﻟ ﺎﺑ و ﯽﺘﻌﻨﺻ ترﻮﺻ ﻪﺑ طﻮﺑﺮﻣ نﺎﺼﺼﺨﺘﻣ ﺮﻈﻧ ﻪﮕﻧ لﻮﺻا ﯽﻣﺎﻤﺗ ﻦﺘﻓﺮﮔ ﺮﻈﻧ رد ﺎﺑ و هﺪﺷ ﺪﯿﻟﻮﺗ نآ ﯽﯾاﺬﻏ يراد
ﻪﺘﺴﺑ رد و يﺪﻨﺑ ﺑ ﺐﺳﺎﻨﻣ يﺎﻫ ﺪﻧدﺮﮔ ﻪﺿﺮﻋ رازﺎﺑ ﻪ
.
يراﺰﮕﺳﺎﭙﺳ :
هﺎﮕﺸﻧاد ﯽﺸﻫوﮋﭘ ﺖﻧوﺎﻌﻣ زا ﻪﻟﺎﻘﻣ نﺎﮔﺪﻨﺴﯾﻮﻧ
حﺮﻃ زا ﯽﻟﺎﻣ ﺖﯾﺎﻤﺣ و ﺐﯾﻮﺼﺗ ﺎﺑ ﻪﮐ ﯽﻠﯿﺑدرا ﻖﻘﺤﻣ
ﯽﺸﻫوﮋﭘ
"
ﻪﺿﺮﻋ ﺪﺠﻨﺳ ردﻮﭘ رد ﯽﻨﯿﺴﮐﻮﺗﻼﻓآ ﯽﮔدﻮﻟآ ﯽﺳرﺮﺑ ﻞﯿﺑدرا رد هﺪﺷ
"
و ﺮﯾﺪﻘﺗ ﺖﯾﺎﻬﻧ ،ﺖﺧﺎﺳ ﺮﯾﺬﭘ نﺎﮑﻣا ار نآ مﺎﺠﻧا
ﺪﻧراد ار ﺮﮑﺸﺗ .
6 - ﻊﺑﺎﻨﻣ
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Aflatoxin Contamination in Retailed Elaeagnus angustifolia Powder in Ardabil
Ghannadiasl, F. 1*, Fathi-Achachlouei, B. 2
1. Assistant Professor, Department of Food Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
2. Associate Professor, Department of Food Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
ABSTRACT ARTIClE INFO
Article History:
Received 26 july 2019 Accepted 22 September 2020
Keywords:
Aflatoxin,
High Performance Liquid Chromatography,
Immunoaffinity column clean-up,
Elaeagnus angustifolia L.
powder
Mycotoxins have been reported to be carcinogenic, teratogenic, genotoxic, hepato- and nephrotoxic. Based on the literature review, there is a need for more studies on the mycotoxin contamination in all types of foods. In this study, 20 samples of Elaeagnus angustifolia L. powder were purchased randomly and clustered based on the distribution of sales areas from different districts in Ardabil city. The determination of aflatoxin contamination was carried out using high performance liquid chromatography and immunoaffinity column clean-up.
According to the results, seventeen of 20 samples (85%) were found contaminated with aflatoxins in the range of 30.40-2360.0 µg/kg. Both AFB1 and AFB2 were detected in 85% of samples. 80% and 70% samples had AFG1 and AFG2, respectively. The contamination levels were exceeded the maximum limit of Iran and European Union. These findings suggest an urgent need to monitor contamination of aflatoxin and consuming population of Elaeagnus angustifolia L.
powder in Iran.
DOI: 10.29252/fsct.18.01.08
*Corresponding Author E-Mail:
Iranian Journal of Food Science and Technology
ir . modares .
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