نﻮﯿﺳﻻﻮﻣﺮﻓ يزﺎﺳ ﻪﻨﯿﻬﺑ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ
ﮏﯿﺗﻮﯿﺑوﺮﭘ
ﻮﻟ ﯽﻔﻄﺼﻣ ﻦﯿﺴﺣ ،1
نﺎﯾژارﺎﮐ ﺎﺿر
2
، *
ﻤﺣا ﺪ ﯽﻃﺎﯿﺘﺣا 3
1 - ﺮﻤﺷﺎﮐ ﯽﻫﺎﮕﺸﻧاددﺎﻬﺟ ﯽﻟﺎﻋ شزﻮﻣآ ﻪﺴﺳﻮﻣ ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ،ﺪﺷرا ﯽﺳﺎﻨﺷرﺎﮐ ﻪﺘﺧﻮﻣآ ﺶﻧاد
، ناﺮﯾا ،ﺮﻤﺷﺎﮐ .
2 - دﺎﺘﺳا ﯾ رﺎ ﺸﻫوﮋﭘ ﯽ
، ﺑ هوﺮﮔ ﯿ ژﻮﻟﻮﻨﮑﺗﻮ ي ﺘﻌﻨﺻ ﯽ ﻣ ﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ
،ﺎﻫ ﺑ هﺪﮑﺸﻫوﮋﭘ ﯿ
ژﻮﻟﻮﻨﮑﺗﻮ ي ﺘﻌﻨﺻ ﯽ
، ﻫﺎﮕﺸﻧاد دﺎﻬﺟ نﺎﻣزﺎﺳ ﯽ
نﺎﺳاﺮﺧ
ﻮﺿر ي
، ناﺮﯾا ،ﺪﻬﺸﻣ .
3 - ﯽﻨﻤﯾا و ﺖﯿﻔﯿﮐ ﯽﺸﻫوﮋﭘ هوﺮﮔ ﻮﻀﻋ ﯽﯾاﺬﻏ داﻮﻣ
نﺎﻣزﺎﺳ ،ﯽﯾاﺬﻏ داﻮﻣ يروﺎﻨﻓ و مﻮﻠﻋ هﺪﮑﺸﻫوﮋﭘ ، دﺎﻬﺟ
ﯽﻫﺎﮕﺸﻧاد نﺎﺳاﺮﺧ
،يﻮﺿر
ناﺮﯾا ،ﺪﻬﺸﻣ .
ﻪﻟﺎﻘﻣ تﺎﻋﻼﻃا ﮑﭼ
ﯿ هﺪ
رﺎﺗ ﯾ ﺎﻫ ﺦ ي ﻪﻟﺎﻘﻣ :
رﺎﺗ ﯾ رد ﺦ ﯾ ﺖﻓﺎ : 12 / 11 / 1399
رﺎﺗ ﯾ ﺬﭘ ﺦ ﯾ شﺮ : 03 / 08 / 1400
ﻠﮐ تﺎﻤﻠﮐ ﯿ
ﺪ ي :
،مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ
،ﺖﻓﺎﺑ ﯽﺘﻔﺳ
،راﻮﮔو ﯽﺑﺮﻋ يﺎﻫ ﻎﻤﺻ ،تﻼﮑﺷ
ﯽﺠﻨﺳ ﺖﻓﺎﺑ .
DOI: 10.52547/fsct.18.120.6
DOR: 20.1001.1.20088787.1400.18.120.6.8
تﺎﺒﺗﺎﮑﻣ لﻮﺌﺴﻣ :
ﻮﻨﻋ ﻪﺑ تﻼﮑﺷ ﯽﮐارﻮﺧ زا ﯽﮑﯾ نا
ﺖﺳﺎﻫاﺬﻏ ﻦﯾﺮﺗراﺪﻓﺮﻃﺮﭘ زا ،هﺰﻤﺷﻮﺧ يﺎﻫ هﺪﻋو رد عﻮﻨﺗ دﺎﺠﯾا ﺎﺑ ﻪﮐ
زوﺮﺑ ﺶﻫﺎﮐ ﺐﺒﺳ ﺐﯿﺗﺮﺗ ﻦﯾا ﻪﺑ و هداد ﺶﯾاﺰﻓا ﻪﻧﺎﺤﺒﺻ فﺮﺻ ﻪﺑ ار داﺮﻓا ﻞﯾﺎﻤﺗ ﺪﻧاﻮﺗ ﯽﻣ ،ﻪﻧﺎﺤﺒﺻ ﯽﯾاﺬﻏ دﻮﺷ زور لﻮﻃ رد ﺎﻬﻧآ ﯽﻟﺎﺣ ﯽﺑ و ﯽﮕﺘﺴﺧ ﻢﺴﯿﻧﺎﮔراوﺮﮑﯿﻣ ﺎﻫ ﮏﯿﺗﻮﯿﺑوﺮﭘ .
ﻪﺑ ﺮﮔا ﻪﮐ ﺪﻨﺘﺴﻫ ﯽﯾﺎﻫ داﺪﻌﺗ
ﻪﺑ و ﯽﻓﺎﮐ ﯽﺘﻣﻼﺳ تاﺮﺛا ،ﺪﻧﺮﯿﮕﺑ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ هﺪﻧز ترﻮﺻ
ﺪﻨﻫد ﯽﻣ زوﺮﺑ دﻮﺧ زا نﺎﺑﺰﯿﻣ رد ﺶﺨﺑ .
هدرواﺮﻓ ﺪﯿﻟﻮﺗ ﻪﺑ نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ زﺎﯿﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯽﺳرﺮﺑ ﺶﻫوﮋﭘ ﻦﯾا زا فﺪﻫ ﺶﺨﺑ ﯽﺘﻣﻼﺳ ﯽﯾاﺬﻏ يﺎﻫ
ﻎﻤﺻ ﯽﮔﮋﯾو ﺮﺑ ﻦﯿﺘﯿﺴﻟ و راﻮﮔ ،ﯽﺑﺮﻋ يﺎﻫ هﺪﻧز و ﯽﻔﯿﮐ يﺎﻫ
ﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ رد ﮏﯿﺗﻮﯿﺑوﺮ
ﺖﺳا ﮏﯿﺗﻮﯿﺑوﺮﭘ .
يرﺎﻣآ حﺮﻃ سﺎﺳا ﺮﺑ رﻮﻈﻨﻣ ﻦﯾا ياﺮﺑ ﻪﺑ
ترﻮﺻ Box-Behnken ،
18 ﻪﯿﻬﺗ ﻪﻧﻮﻤﻧ
تﺎﺒﯿﮐﺮﺗ ﻞﻣﺎﮐ طﻼﺘﺧا زا ﺪﻌﺑ ﻪﮐ ﺪﯾدﺮﮔ )
ﻎﻤﺻ ،ﻞﻫ ،رﻮﮕﻧا هﺮﯿﺷ ،هدرا ،ﻞﺴﻋ ﻞﻣﺎﺷ و راﻮﮔ ،ﯽﺑﺮﻋ يﺎﻫ
ﻦﯿﺘﯿﺴﻟ ( ﺑﻮﺘﮐﻻ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ،ﺖﺧاﻮﻨﮑﯾ ﺖﻓﺎﺑ ﻪﺑ ﯽﺑﺎﯿﺘﺳد و ﻪﻠﺣﺮﻣ رد ﺮﻈﻧ درﻮﻣ مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎ
ناﺰﯿﻣ ﻪﺑ ﺮﺧآ 108
ﮏﻨﺧ و ﮏﺸﺧ ﻞﺤﻣ رد و ﻪﯿﻬﺗ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ ﺖﯾﺎﻬﻧ رد و ﺪﺷ ﻪﻓﺎﺿا مﺮﮔ ﺮﻫ رد
ﺪﻧﺪﺷ يراﺪﻬﮕﻧ .
نﻮﻣزآ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ شرﺎﻤﺷ و ﺖﻓﺎﺑ ﯽﺘﻔﺳ ،ﻪﺘﯾزﻮﮑﺴﯾو ﻞﻣﺎﺷ ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ
زا ﺪﻌﺑ ﻪﮐ دﻮﺑ حﺮﻃ
ر ﯾﺰ ي ﺎﻣزآ ﯾ ﺶ
،ﺎﻫ لﺪﻣ زﺎﺳ ي ﺳﺮﮔر ﯿ ﻧﻮ ﯽ رﻮﮕﻟا ﺎﺑ ﯾﺘ ﻢ ﺲﭘ فﺬﺣ ﻬﺑ ،رﻮﺧ
ﯿ ﻪﻨ زﺎﺳ ي دﺪﻋ ي
ﺎﻫرادﻮﻤﻧ ﻢﺳر و ي
مﺮﻧ ﺎﺑ ﺦﺳﺎﭘ ﺢﻄﺳ راﺰﻓا
Design-Expert مﺎﺠﻧا
ﺪﯾدﺮﮔ . نآ زا ﯽﮐﺎﺣ ﺶﻫوﮋﭘ ﺞﯾﺎﺘﻧ
ﯽﺘﻓﺎﺑ يﺎﻫ ﯽﮔﮋﯾو ﺮﺑ ﻦﯿﺘﯿﺴﻟ و راﻮﮔ ﻎﻤﺻ ندوﺰﻓا ﻪﮐ دﻮﺑ )
ﻪﺘﯾزﻮﮑﺴﯾو و ﺖﻓﺎﺑ ﯽﺘﻔﺳ (
ﯽﻧﺎﻣ هﺪﻧز و
مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻟﺎﺣرد ،ﺖﺳا رادﺎﻨﻌﻣ
ﺮﯿﺛﺎﺗ ﻦﯿﺘﺴﯿﻟ و ﯽﺑﺮﻋ ﻎﻤﺻ ندوﺰﻓا ﻪﮐ
سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز رد يرادﺎﻨﻌﻣ ﺶﯾاﺰﻓا و ﻪﺘﯾزﻮﮑﺴﯾو ،ﺖﻓﺎﺑ ﯽﺘﻔﺳ ﺮﺑ يرادﺎﻨﻌﻣ ﺖﺷاﺪﻧ مورﺎﺘﻧﻼﭘ .
ﺷ ﻞﮐ ﺖﯿﺑﻮﻠﻄﻣ ﺎﺑ ﺶﻫوﮋﭘ ﻦﯾا رد ﻪﻨﯿﻬﺑ ﻂﯾاﺮ ﻪﻃﻮﺑﺮﻣ يرﺎﻣآ حﺮﻃ سﺎﺳا ﺮﺑ ،
ﺖﻈﻠﻏ رد
564 /0 ،ﻦﯿﺘﺴﯿﻟ 8 /0 و راﻮﮔ 064 /0 هﺪﻧز ﻦﯾﺮﺘﺸﯿﺑ ﯽﺑﺮﻋ ﻎﻤﺻ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ
cfu/gr 589
/ 10 و
ﺖﻓﺎﺑ ﯽﺘﻔﺳ 22
/8 ﺪﺷ ﻢﻫاﺮﻓ ﻦﺗﻮﯿﻧ .
ناﺮﯾا ﯽﯾاﺬﻏ ﻊﯾﺎﻨﺻ و مﻮﻠﻋ ﻪﻠﺠﻣ
www.fsct.modares.ac.ir:ﻪﻠﺠﻣ ﺖﯾﺎﺳ
ﯽﻤﻠﻋ ﻪﻟﺎﻘﻣ ﯽﺸﻫوﮋﭘ_
1 - ﻪﻣﺪﻘﻣ
ﮏﯾ ناﻮﻨﻋ ﻪﺑ تﻼﮑﺷ ﯽﮐارﻮﺧ
،هﺰﻤﺷﻮﺧ و دﺮﻓ ﻪﺑ ﺮﺼﺤﻨﻣ
ﺮﺛا ﻪﮐ ﺖﺳا ﯽﮑﯾژﻮﻟﻮﯿﺑ لﺎﻌﻓ داﻮﻣ ﻢﻬﻣ ﻊﺑﺎﻨﻣ زا ﯽﮑﯾ ﯽﺘﻧآ هﮋﯾو ﯽﻧاﺪﯿﺴﮐا ﺖﻣﻼﺳ ﺮﺑ و هداد نﺎﺸﻧ نﺎﺴﻧا نﺪﺑ رد ار يا
ﺐﻠﻗ هﮋﯾو ﻪﺑ نﺪﺑ ﻒﻠﺘﺨﻣ يﺎﻀﻋا ﯿﺛﺄﺗ قوﺮﻋ و
ﯽﯾاﺰﺴﺑ ﺮ دراد
] 1 [ و ﯽﺘﻧآ ناﺪﯿﺴﮐا اﺬﻏ رد دﻮﺟﻮﻣ يﺎﻫ
، ﺮﺑاﺮﺑ رد ار نﺪﺑ
لﺎﮑﯾدار ﯽﻣ ﺖﻈﻓﺎﺤﻣ دازآ يﺎﻫ ﺪﻨﻨﮐ
. ﻊﺑﺎﻨﻣ زا ﯽﮑﯾ ﻮﺋﺎﮐﺎﮐ
ﯽﺘﻧآ هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﻦﯿﭽﺗﺎﮐ و ﺖﺳا ناﺪﯿﺴﮐا
تﻼﮑﺷ رد دﻮﺟﻮﻣ 1
ﯽﻣ ﺎﻫﺪﯿﺋﻮﻧوﻼﻓ هداﻮﻧﺎﺧ زا ﻪﮐ ﻦﯾﺮﺘﯾﻮﻗ ءﺰﺟ ،ﺪﺷﺎﺑ
ﯽﺘﻧآ ناﺪﯿﺴﮐا ﺖﺳﺎﻫ . ﺎﻘﻘﺤﻣ ﻪﺘﻓﺎﯾرد ن رد ﻪﮐ ﺪﻧا 100
زا مﺮﮔ
،ﺦﻠﺗ تﻼﮑﺷ 5/
3 راﺪﻘﻣ ﻦﯾا ﻪﮐ دراد دﻮﺟو ﻦﯿﭽﺗﺎﮐ مﺮﮔ ﯽﻠﯿﻣ
ﺖﺳا يﺎﭼ رد نآ راﺪﻘﻣ ﺮﺑاﺮﺑ رﺎﻬﭼ .
ﻦﯿﻣآﺮﯿﺗ يوﺎﺣ ﻮﺋﺎﮐﺎﮐ ﮥﻧاد
ﯽﻣ ﻦﯿﻣآ ﻞﯿﺗا ﻞﯿﻨﻓ و ﺶﯾاﺰﻓا ﺚﻋﺎﺑ داﻮﻣ ﻦﯾا ود ﺮﻫ ﻪﮐ ﺪﺷﺎﺑ
ﯽﻣ يرﺎﯿﺷﻮﻫ ﻪﺟرد ﺪﻧدﺮﮔ
. رد دﻮﺟﻮﻣ هﺪﻤﻋ ﯽﺑﺮﭼ ﻮﺋﺎﮐﺎﮐ هﺮﮐ
تﻼﮑﺷ ﺖﺳا ] 2[
. ﯽﺑﺮﭼ ﯽﺧﺮﺑ ار نﻮﺧ لوﺮﺘﺴﻠﮐ ،ﯽﺋاﺬﻏ يﺎﻫ
ﯽﻣ ﺶﯾاﺰﻓا يﺮﺗ نﺎﻤﺘﺧﺎﺳ ﻞﯿﻟد ﻪﺑ ﻮﺋﺎﮐﺎﮐ ةﺮﮐ ﯽﻟو ﺪﻨﻫد
ﯿﺴﯿﻠﮔ ﺖﺳا ﯽﺘﯿﺻﻮﺼﺧ ﻦﯿﻨﭼ ﺪﻗﺎﻓ يﺪﯾﺮ ]
3 [.
و ﻞﻣﺎﮐ ﺪﻨﯾاﺮﻓ ﻞﺻﺎﺣ ناﺮﯾا دراﺪﻧﺎﺘﺳا يﺎﻨﺒﻣ ﺮﺑ تﻼﮑﺷ هدرواﺮﻓ ﺪﻨﭼ ﺎﯾ ﮏﯾ طﻮﻠﺨﻣ زا ﯽﺤﯿﺤﺻ ﻮﺋﺎﮐﺎﮐ ﻪﻧاد ﺰﻐﻣ زا
دﻮﭘ ،ﻮﺋﺎﮐﺎﮐ ﺮﯿﻤﺧ ﻞﻣﺎﺷ ﯽﻣ ﻮﺋﺎﮐﺎﮐ هﺮﮐ و ﻮﺋﺎﮐﺎﮐر
ﻦﮑﻤﻣ ﻪﮐ ﺪﺷﺎﺑ
هﺪﻨﻨﮐ ﻦﯾﺮﯿﺷ ﺎﯾ و ﺮﮑﺷ هاﺮﻤﻫ ﺖﺳا ،ﯽﮐارﻮﺧ زﺎﺠﻣ يﺎﻫ
هدرواﺮﻓ ﯽﻧدوﺰﻓا و يﺮﯿﺷ يﺎﻫ ﺪﺷﺎﺑ زﺎﺠﻣ يﺎﻫ
] 4 [ و ﻊﻗاو رد
و ﻮﺋﺎﮐﺎﮐ هﺮﮐ ار ﻊﯾﺎﻣ زﺎﻓ نآ رد ﻪﮐ ﺖﺳا يﺪﯿﺋﻮﻠﮐ ﻢﺘﺴﯿﺳ ﯽﻋﻮﻧ ﯽﻣ ﻞﯿﮑﺸﺗ ﺮﮑﺷ و ﻮﺋﺎﮐﺎﮐ ردﻮﭘ تارذ ار هﺪﻨﮐاﺮﭘ زﺎﻓ ﺪﻨﻫد
.
تﻼﮑﺷ ﻞﯿﺗا ﻞﯿﻨﻓ و ﻦﯿﻣوﺮﺑﻮﺌﺗ ،ﻦﯿﺌﻓﺎﮐ هدﺎﻣ ﻪﺳ ﻦﺘﺷاد ﻞﯿﻟد ﻪﺑ ﺶﻣارآ و ﻒﻌﺷ سﺎﺴﺣا ﺐﺟﻮﻣ و هدﻮﺑ ﻢﯾﻼﻣ كﺮﺤﻣ ﻦﯿﻣآ
ﯽﻣ ﯽﺸﺨﺑ ددﺮﮔ
] 5[
.
ﺑوﺮﭘ ﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ﻣ ﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي هﺪﻧز ا ي فﺮﺼﻣ ﻪﮐ ﺪﻨﺘﺴﻫ
دﺎﻘﻣ رد ﺎﻬﻧآ ﯾﺮ
ﻓﺎﮐ ﯽ
، ا ﺐﺒﺳ ﯾ دﺎﺠ اﺮﺑ ﺶﺨﺑ ﺖﻣﻼﺳ صاﻮﺧ ي
ﻣﯿ نﺎﺑﺰ ﻣ ﯽ دﻮﺷ ] 6 [ .ا ﯾ دﺎﺠ ﺖﻣﻼﺳ صاﻮﺧ ﺑوﺮﭘ ﺶﺨﺑ
ﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ
1. Catechin
ﺪﻣ ًﺎﺳﺎﺳا ﯾ نﻮ ﺎﻫﺮﺛا ي ز رﻮﻠﻓ ﺮﺑ ﺎﻬﻧآ هﺪﻨﻨﮐ بﻮﮐﺮﺳ ﯾ
نﺎ روآ هدور
اﻮﺗ دﻮﺒﻬﺑ و ﻆﻔﺣ و نز
ا ﯾ ﻦ ﻮﻘﺗ ،رﻮﻠﻓ ﯾ ﺖ ﺳ ﯿ ﻢﺘﺴ ا ﯾ ﻨﻤ ﯽ
، ﺶﻫﺎﮐ
و ،نﻮﺧ رﺎﺸﻓ ﺶﻫﺎﮐ ،مﺮﺳ لوﺮﺘﺴﻠﮐ ﺢﻄﺳ ...
ﺖﺳا ] 7 [ .
ﺎﻫاﺬﻏ ي وﺎﺣ ي ﺑوﺮﭘ ﯿ ﺗﻮ ﯿ ﮏ ﺎﺑ ﯾﺪ ا ﯾ ﻦﻤ وﺎﺣ فﺮﺼﻣ نﺎﻣز رد و ي
دﺎﻘﻣ ﯾﺮ ﻓﺎﮐ ﯽ ا زا ﯾ ﻦ ﻣ ﯿ ﮔراوﺮﮑ ﻧﺎﯿ ﻢﺴ ﺎﻫ ﺪﻨﺷﺎﺑ . راﺪﻘﻣ ﻞﻗاﺪﺣ
ﺑوﺮﭘ ﯿ ﺗﻮ ﯿ ﮏ ﺎﻫاﺬﻏ رد ي
وﺎﺣ ي ﺑوﺮﭘ ﯿ ﺗﻮ ﯿ ﮏ ﺎﺑ ﯾﺪ cfu/gr
107 ﺪﺷﺎﺑ . يﺮﺘﮐﺎﺑ زا ﯽﮔرﺰﺑ هوﺮﮔ ﻪﺑ ﻖﻠﻌﺘﻣ ﺎﻫ ﮏﯿﺗﻮﯿﺑوﺮﭘ ﺮﺜﮐا
ﯽﮔﺪﻧز ﺎﺠﻧآ رد و هدﻮﺑ نﺎﺴﻧا هدور ﯽﺑوﺮﮑﯿﻣ رﻮﻠﻓ ﯽﻠﺻا يﺎﻫ ﻣآ ﺖﻤﻟﺎﺴﻣ ﯿﺰ
ﺮﺗ ﻢﻬﻣ ﻪﮐ ﺪﻧراد يرﺮﺿ ﯽﺑ و ﯾ
ﻦ مﻮﺳﺮﻣ و ﺮﺗ ﯾ ﻦ
ﺑوﺮﭘ ﯿ ﺗﻮ ﯿ ﮏ ﺎﻫ ﺟ ﻪﺑ ﺳﺎﺑﻮﺘﮐﻻ ﺲﻨ ﯿ
سﻮﻠ ﺑ و ﯿﻔ ﯿ ﺮﺘﮐﺎﺑوﺪ ﯾ مﻮ ﻖﻠﻌﺗ
ﺪﻧراد . ﻮﺳ ﯾﻪ ﺎﻫ ي ﺳﺎﺑﻮﺘﮐﻻ ﯿ سﻮﻠ ﺳا ﯿ ﻓوﺪ ﯿ
،سﻮﻠ ﺳﺎﺑﻮﺘﮐﻻ ﯿ سﻮﻠ
زﺎﮐ ﯽﯾ
، ﺳﺎﺑﻮﺘﮐﻻ ﯿ سﻮﻠ ﺑ و سﻮﺳﻮﻨﻣار ﯿﻔ
ﯿ ﺮﺘﮐﺎﺑوﺪ ﯾ مﻮ ﺑ ﯿ ﻔﯿ ﺪ ﯾ مﻮ
ﺑﯿ ﺮﺘﺸ ﯾ ﻦ رﺎﺠﺗ دﺮﺑرﺎﮐ ي
ﺪﻧراد ار ] 8[
. زا ﻊﻧﺎﻣ ﺎﻫ يﺮﺘﮐﺎﺑ ﻦﯾا
ﯽﻣ هدور ﺶﺷﻮﭘ بﺎﻬﺘﻟا زا ار هدور ﺶﺷﻮﭘ ﻦﯿﻨﭽﻤﻫ ،ﺪﻧﻮﺷ
ﻠﻤﺣ نژﻮﺗﺎﭘ ﻢﺟﺎﻬﺗ و ﻪ ﺎﻫ
ﺦﺳﺎﭘ ﻢﯿﻈﻨﺗ ﺚﻋﺎﺑ و ﺪﻨﻨﮐ ﯽﻣ ﺖﻇﺎﻔﺣ
ﺪﻧﻮﺷ ﯽﻣ هدور ﯽﻨﻤﯾا ﻢﺘﺴﯿﺳ ]
8[
.
ﻎﻤﺻ ﯽﺑﺮﻋ ﯽﻣ ﺎﯿﺳﺎﮐآ عاﻮﻧا زا ﯽﺘﺧرد زا هﺪﺷ شواﺮﺗ هدﺎﻣ
ﻪﺑ نآ ﺖﺳﻮﭘ ﻪﺑ نﺪﺷ دراو ﻪﻣﺪﺻ ﺮﺛا رد ﻪﮐ ﺪﺷﺎﺑ ﺖﺳد
ﯽﻣ ﺪﯾآ
ﯽﺘﻧﺎﺳ ﺪﻨﭼ ﺮﻄﻗ ﻪﺑ ﯽﺗاﺮﻄﻗ ترﻮﺻ ﻪﺑ و اﻮﻫ نﺎﯾﺮﺟ رد ﻪﮐ ﺮﺘﻣ
ﻤﺟ ﺪﻧا هﺪﺷ ﮏﺸﺧ ﯽﻣ يروآ ﻊ
دﻮﺷ
،ﮓﻧرﺪﯿﻔﺳ هدﺎﻣ ﻦﯾا . رد
ﺖﺳا لﻮﻠﺤﻣ رﺎﯿﺴﺑ بآ يﺎﻫ ﺪﺣاو زا و
ﯽﻧﺎﻤﺘﺧﺎﺳ - L
،زﻮﻨﯿﺑارآ
-L
،زﻮﻨﻣار - D و زﻮﺘﮐﻻﺎﮔ - D
ﺪﯿﺳا ﮏﯿﻧورﻮﮐﻮﻠﮔ ﻞﯿﮑﺸﺗ
ﺖﺳا هﺪﺷ . ﻎﻤﺻ ﻦﯾا رد
ﻢﺘﺴﯿﺳ يﺎﻫ ﯽﯾاﺬﻏ ﻪﺑ ﻪﻟﺰﻨﻣ نﻮﯿﺴﻟﻮﻣا
ﯽﻣ هدﺎﻔﺘﺳا هﺪﻨﻨﮐ راﺪﯾﺎﭘ و هﺪﻨﻨﮐ دﻮﺷ
] 9 [.
ﯿﮔ ﻪﻧاد زا راﻮﮔ ﻎﻤﺻ هﺎ
Cyamopsis tetragonolobus
ﺪﯾآ ﯽﻣ ﺖﺳﺪﺑ .
ﯽﻟﻮﮑﻟﻮﻣ نزو ﻪﺑ يﺪﯾرﺎﮐﺎﺳ ﯽﻠﭘ زا ﻎﻤﺻ ﻦﯾا
50000 ﺎﺗ 80000000 ﺖﺳا هﺪﺷ ﻞﯿﮑﺸﺗ نﻮﺘﻟاد
. نآ نﺎﻤﺘﺧﺎﺳ
زا ﺣاو زا ﻞﮑﺸﺘﻣ يﺪﯾرﺎﮐﺎﺳ ﯽﻠﭘ ﻪﮐ ﺖﺳا نﺎﻧﺎﻣﻮﺘﮐﻻﺎﮔ ﺪ
يﺎﻫ
يد يد و زﻮﺘﮐﻻﺎﮔ ﺎﺘﺑ ﺪﻧﻮﯿﭘ ﺎﺑ ﻮﻧﺎﻣ
6 - 1 ﺖﺳا هﺪﺷ ﻞﯿﮑﺸﺗ ]
10 [.
ﻟﺎﻄﻣ نﻮﻨﮐﺎــﺗ ﯽﻨﯾﺰﮕﯾﺎــﺟ صﻮــﺼﺧ رد يدوﺪــﺤﻣ تﺎــﻌ
يﺮﭘ تﺎﺒﯿﮐﺮﺗ ﺎﺑ زورﺎﮐﺎﺳ ﻢـﺠﺣ و ﮏـﯿﺗﻮﯿﺑ
رﻮـﻈﻨﻣ ﻪـﺑ هﺪـﻨﻫد
ﺖـﺳا ﻪـﺘﻓﺮﮔ ترﻮﺻ تﻼﮑﺷ يﺮﻟﺎﮐ ﺶﻫﺎﮐ و نﺎﻫاﻮﺧﺮﯿﺧ
نارﺎﮑﻤﻫ )
1396 ( ﮏﯿﺗﻮﯿﺑوﺮﭘ تﻼﮑﺷ ﺪﯿﻟﻮﺗ ﯽﺠﻨﺳ نﺎﮑﻣا
يﺮﺘﮐﺎﺑ يوﺎﺣ Bifidobacterium breve
هﺪﺷ ﯽﻧﺎﺷﻮﭘﺰﯾر
ﺎﺸﻧ و ﻢﯿﺴﻠﮐ تﺎﻨﯾﮋﻟآ ﺎﺑ شور ﻪﺑ ترذ موﺎﻘﻣ ﻪﺘﺳ
و نﻮﯿﺴﻟﻮﻣا
نآ ﯽﻧﺎﻣ هﺪﻧز ناﺰﯿﻣ ﯽﺳرﺮﺑ ﺪﻧداد راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ار
. ﻦﯾا رد
تﺎﻨﯾﮋﻟآ زا ،ﻪﻌﻟﺎﻄﻣ ﯿﺴﻠﮐ
موﺎﻘﻣ ﻪﺘﺳﺎﺸﻧ و ﻢ ذ
دﺎﺠﯾا ياﺮﺑ تر
و ﺪﺷ هدﺎﻔﺘﺳا نﻮﯿﺴﻟﻮﻣا شور ﻪﺑ ﺎﻫ لﻮﺴﭙﮐ يﺮﺘﮐﺎﺑ ﻦﯾا
ﻪﺑ
ﯽﻧﺎﺷﻮﭘ ﺰﯾر و دازآ ﺖﻟﺎﺣ هﺪﻧز و ﺪﺷ هدوﺰﻓا تﻼﮑﺷ ﻪﺑ هﺪﺷ
،ﯽﻧﺎﻣ آ ﺖﯿﻟﺎﻌﻓ ،ﻪﺘﯾﺪﯿﺳا ﯽﺑ
) aw
( و ﺪﻣﺎﺟ داﻮﻣ يﻮﺘﺤﻣ ،
ﯽﮔﮋﯾو ﯽﻃ تﻼﮑﺷ ﯽﺴﺣ يﺎﻫ 30
يﺎﻣد رد يراﺪﻬﮕﻧ زور 4
داﺮﮕﯿﺘﻧﺎﺳ ﻪﺟرد ﺖﻓﺮﮔ راﺮﻗ ﯽﺑﺎﯾزرا درﻮﻣ
. ﺰﯾر ﮏﯿﺗﻮﯿﺑوﺮﭘ
هﺪﻧز ،دازآ ﺖﻟﺎﺣ ﻪﺑ ﺖﺒﺴﻧ هﺪﺷ ﯽﻧﺎﺷﻮﭘ رد يﺮﺘﺸﯿﺑ ﯽﻧﺎﻣ
ﺖﺷاد تﻼﮑﺷ .
ﺪﯿﺳا دازآ ﺖﻟﺎﺣ ﻪﺑ يﺮﺘﮐﺎﺑ يوﺎﺣ تﻼﮑﺷ ﯾ
ﻪﺘ
ﺖﯿﻟﺎﻌﻓ و ﺮﺗﻻﺎﺑ ﯽﺑآ
، ﺪﻣﺎﺟ داﻮﻣ يﻮﺘﺤﻣ ﺮﺘﻤﮐ
ﻪﻧﻮﻤﻧ ﻪﺑ ﺖﺒﺴﻧ
هﺪﺷ ﯽﻧﺎﺷﻮﭘ ﺰﯾر ﮏﯿﺗﻮﯿﺑوﺮﭘ ﺖﺷاد
ﯽﻧﺎﺷﻮﭘ ﺰﯾر نآ ﺮﺑ هوﻼﻋ ،
ﯽﮔﮋﯾو ﺮﺑ ﯽﺑﻮﻠﻄﻣﺎﻧ ﺮﺛا ﺖﺷاﺪﻧ تﻼﮑﺷ ﯽﺴﺣ يﺎﻫ
] 1 [.
لاﺪﻨﻣ و نارﺎﮑﻤﻫ ) 2012 (،
تﻼﮑﺷ يﺮﯿﺷ ﻦﯿﺳ ﮏﯿﺗﻮﯿﺑ ار
ﻂﺳﻮﺗ يﺮﺘﮐﺎﺑ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ ﯽﺋزﺎﮐ
NCDC 298
ﻪﻟﻮﺴﭙﮐ و هﺪﺷ ﻪﻟﻮﺴﭙﮐ هﺪﺸﻧ
ﺪﯿﻟﻮﺗ هدﺮﮐ و زا ﻦﯿﻟﻮﻨﯾا ﻪﺑ ناﻮﻨﻋ
هدﺎﻣ يﺮﭘ ﮏﯿﺗﻮﯿﺑ هﺮﻬﺑ ﺪﻧدﺮﺑ . ﺞﯾﺎﺘﻧ ﻦﯾا ﻖﯿﻘﺤﺗ نﺎﺸﻧ داد ﻪﮐ
داﺪﻌﺗ يﺮﺘﮐﺎﺑ يﺎﻫ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ ﯽﺋزﺎﮐ
ﺎﺗ 60 زور يراﺪﻬﮕﻧ
ﺖﺤﺗ يراﺪﻬﮕﻧ ﻂﯾاﺮﺷ رد
لﺎﭽﺨﯾ ﺮﺘﺸﯿﺑ زا log cfu/g 108
دﻮﺑ . توﺎﻔﺗ ﻦﯿﻨﭽﻤﻫ يﺮﯿﮕﻤﺸﭼ
رد هﺪﻧز ﯽﻧﺎﻣ
سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ يﺎﻫ
ازآ د ﻪﻟﻮﺴﭙﮐ و هﺪﺷ
رد تﻼﮑﺷ يﺮﯿﺷ ﯽﻃ
يراﺪﻬﮕﻧ رد لﺎﭽﺨﯾ هﺪﻫﺎﺸﻣ و ﺪﯾدﺮﮕﻧ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ
ﻪﻟﻮﺴﭙﮐ
هﺪﺷ ﺚﻋﺎﺑ ﺶﯾاﺰﻓا سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ و ﺎﻫ
ﺶﻫﺎﮐ مﺮﻔﯿﻠﮐ يﺎﻫ
ﻋﻮﻓﺪﻣ ﯽ و ﺖﯿﻟﺎﻌﻓ ﺎﺘﺑ زاﺪﯿﻧورﻮﮐﻮﻠﮔ - ﺪﺷ
] 12 [.
2 - شور و داﻮﻣ ﺎﻫ
2 - 1 - مزﻻ داﻮﻣ
ﺎﻫ ﻪﻧﻮﮔ ي ﺳﺎﺑﻮﺘﮐﻻ ﯿ سﻮﻠ مورﺎﺘﻧﻼﭘ )
1896 (PTCC ﺻ ﻪﺑ ترﻮ
ﮏﺗ ﯾﻮﺳ
،ﻪ دﺎﻤﺠﻧا هﺪﺷ ﮏﺸﺧ و ﺺﻟﺎﺧ ي
ﺴﮑﻠﮐ ﺰﮐﺮﻣ زا ﯿ
نﻮ ﻣﯿ
ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﺎﻫ ي ﺘﻌﻨﺻ ﯽ ﺎﻫ ﺶﻫوﮋﭘ نﺎﻣزﺎﺳ ي
ﻤﻠﻋ ﯽ و
ﺘﻌﻨﺻ ﯽ ا ﯾ ناﺮ ﺴﻟ ﯿﺘﯿ ﻦ ﻞﯿﺟرﺎﮐ ﺖﮐﺮﺷ زا
1
،ﺪﻨﻠﻫ2
ﻎﻤﺻ ﺑﺮﻋ و ﯽ
ﻎﻤﺻ راﻮﮔ نﺎﻤﻟآ يﺪﻧﻮﮐ ﺖﮐﺮﺷ زا ﺗ 2
ﻬﯿ ﻪ دﺮﮔ ﯾﺪ .
2 - 2 - ﻪﯿﻬﺗ شور ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ
هدرا ،ﻞﺴﻋ هﺪﺷ ﻪﺋارا لﻮﻣﺮﻓ ﺎﺑ ﺐﺳﺎﻨﺘﻣ ردﻮﭘ ﺎﺑ رﻮﮕﻧا هﺮﯿﺷ و
ﻞﯿﮔرﺎﻧ ردﻮﭘ و ﻞﻫ ،ﻞﻣﺎﮐ طﻼﺘﺧا زا ﺲﭘ و ﺪﺷ طﻮﻠﺨﻣ ﻮﺋﺎﮐﺎﮐ ﺮﻈﻧ درﻮﻣ ﻎﻤﺻ و ﻦﯿﺘﺴﻟ ﺲﭙﺳ و هﺪﺷ ﻪﻓﺎﺿا )
ﺎﯾ ﯽﺑﺮﻋ ﻎﻤﺻ
راﻮﮔ ﻎﻤﺻ (
ﺪﺷ ﻪﻓﺎﺿا . ﻞﻣﺎﮐ طﻼﺘﺧا ﺎﺗ ﻪﻘﯿﻗد ﺪﻨﭼ ياﺮﺑ ندﺰﻤﻫ
ﺖﺧاﻮﻨﮑﯾ ﺖﻓﺎﺑ ﻪﺑ ﯽﺑﺎﯿﺘﺳد زا ﺲﭘ و ﻪﺘﻓﺎﯾ ﻪﻣادا تﺎﺒﯿﮐﺮﺗ درﻮﻣ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﺲﭘ و ﺪﺷ ﻪﻓﺎﺿا ﺮﺧآ ﻪﻠﺣﺮﻣ رد ﺮﻈﻧ
تﺎﺒﯿﮐﺮﺗ ﻪﯿﻠﮐ هﺪﺷ طﻮﻠﺨﻣ زا نﺎﻨﯿﻤﻃا و ﯽﯾﺎﻬﻧ ندز ﻢﻫ زا ﺪﺷ ﻪﯿﻬﺗ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ .
ﺮﻈﻧ درﻮﻣ يﺎﻫ نﻮﻣزآ مﺎﺠﻧا ﺖﻬﺟ
رد ﺎﻫ نﻮﻣزآ مﺎﺠﻧا نﺎﻣز ﺎﺗ و هﺪﺷ ﻢﯿﺴﻘﺗ راد برد فوﺮﻇ رد ﺪﻧﺪﺷ يراﺪﻬﮕﻧ ﮏﻨﺧ و ﮏﺸﺧ ﻞﺤﻣ )
لوﺪﺟ 1 (.
2 - 3 - لﺎﻌﻓ ﯽﻧﺎﺷﻮﭘﺰﯾر و يزﺎﺳ )
ﻮﺴﭙﮐ نﻮﯿﺳﻻ (
يﺮﺘﮐﺎﺑ ﮏﯿﺗﻮﯿﺑوﺮﭘ
ﺪﺷ ﯽﻧﺎﺷﻮﭘﺰﯾر نﻮﯿﺴﻟﻮﻣا شور زا هدﺎﻔﺘﺳا ﺎﺑ ﮏﯿﺗﻮﯿﺑوﺮﭘ .
ﻦﯾﺪﺑ
اﺪﺘﺑا ﻪﮐ ترﻮﺻ 2
ﻢﯾﺪﺳ تﺎﻨﯾﮋﻟآ مﺮﮔ )
ﺖﮐﺮﺷ Sigma-
Aldrich ﺎﮑﯾﺮﻣآ ، ( و 1 ﺖﮐﺮﺷ ترذ موﺎﻘﻣ ﻪﺘﺳﺎﺸﻧ مﺮﮔ
) national starch
نﺎﺘﺴﻠﮕﻧا ، (
رد ﯽﻣارآ ﻪﺑ 100ml
ﺮﻄﻘﻣ بآ
ﻼﮐﻮﺗا رد ﺲﭙﺳ و ﺪﺷ ﻞﺣ ًﻼﻣﺎﮐ ﻪﮑﻧآ زا ﺲﭘ ،ﺪﺷ ﻞﯾﺮﺘﺳا و
ﺎﺑ تﺎﻨﯾﮋﻟآ لﻮﻠﺤﻣ ،ﺪﺷ ﺎﻣد ﻢﻫ ﻂﯿﺤﻣ ﺎﺑ لﻮﻠﺤﻣ 1ml
تﺪﻣ ﻪﺑ ﯽﺑوﺮﮑﯿﻣ نﻮﯿﺴﻧﺎﭙﺳﻮﺳ 5
ﺪﺷ طﻮﻠﺨﻣ ﻪﻘﯿﻗد .
ياﺮﺑ
نﻮﯿﺴﻟﻮﻣا ﻞﯿﮑﺸﺗ ﻪﺑ ﻪﻠﺻﺎﺣ طﻮﻠﺨﻣ
500 ml ترذ ﻦﻏور
يوﺎﺣ 2/
0 ﻦﯿﺋﻮﺗ ﺮﯾﺎﻔﯿﺴﻟﻮﻣا ﺪﺻرد 80
) ﺖﮐﺮﺷ Merck ،
نﺎﻤﻟآ ( ﯽﺴﯿﻃﺎﻨﻐﻣ نﺰﻤﻫ زا هدﺎﻔﺘﺳا ﺎﺑ و ﻪﻓﺎﺿا ﺖﻋﺮﺳ ﺎﺑ
) rpm
350 ( تﺪﻣ ﻪﺑ 40 نﻮﯿﺴﻟﻮﻣا و ﺪﺷ هﺪﻨﮐاﺮﭘ ﻞﻣﺎﮐ رﻮﻃ ﻪﺑ ﻪﻘﯿﻗد
ﺪﺷ ﻞﯿﮑﺸﺗ ﯽﺘﺧاﻮﻨﮑﯾ .
لﻮﺴﭙﮐ ﻞﯿﮑﺸﺗ رﻮﻈﻨﻣ ﻪﺑ لﻮﻠﺤﻣ ﻪﺑ ،ﺎﻫ
،ﺮﻈﻧ درﻮﻣ 500ml
ﺪﯾﺮﻠﮐ ﻢﯿﺴﻠﮐ 1/ M 0 تﺪﻣ ﻪﺑ و ﺪﺷ ﻪﻓﺎﺿا
1 . Cargill 2. Condio
1 ﺪﺷ هدﺰﻤﻫ ﺮﮕﯾد ﻪﻘﯿﻗد .
طﻮﻠﺨﻣ ﺎﺗ ﺪﺷ ﻒﻗﻮﺘﻣ ندﺰﻤﻫ ﺲﭙﺳ
زا ﺲﭘ دﻮﺷ زﺎﻓ ود ﻞﺻﺎﺣ 30
ﮐ ﻪﻘﯿﻗد لﻮﺴﭙ ﺪﻧﺪﺷ ﻦﯿﺸﻧ ﻪﺗ ﺎﻫ .
لﻮﺴﭙﮐ يزﺎﺳ اﺪﺟ رﻮﻈﻨﻣ ﻪﺑ ﻸﺧ ﺖﺤﺗ ﺮﻨﺧﻮﺑ ﻒﯿﻗ زا ،ﺎﻫ
لﻮﺴﭙﮐ ﺲﭙﺳ ،ﺪﺷ هدﺎﻔﺘﺳا ﺮﻄﻘﻣ بآ لﻮﻠﺤﻣ ﺎﺑ هﺪﺷ اﺪﺟ يﺎﻫ
و ﺪﻧﺪﺷ ﻪﺘﺴﺷ نﺎﮑﻣا ﺪﺣ ﺎﺗ ﻦﻏور يزﺎﺳاﺪﺟ رﻮﻈﻨﻣ ﻪﺑ ﻞﯾﺮﺘﺳا يﺎﻣد رد 4 ﯽﺘﻧﺎﺳ ﻪﺟرد ﺪﻧﺪﺷ يراﺪﻬﮕﻧ داﺮﮔ
] 19 [.
2 - 4 - ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ نﻮﻣزآ
2 - 4 - 1 - زاﺪﻧا ﯽﺑآ ﺖﯿﻟﺎﻌﻓ يﺮﯿﮔ ه
ﺖﯿﻟﺎﻌﻓ ﺎﮕﺘﺳد ﻂﺳﻮﺗ تﻼﮑﺷ يﺎﻫ ﻪﻧﻮﻤﻧ ﯽﺑآ ه
Novasina
Sprint ) لﺪﻣ 500 ﺲﯿﺋﻮﺳ ﺖﺧﺎﺳ (
ﺪﯾدﺮﮔ ﻦﯿﯿﻌﺗ .
زا يراﺪﻘﻣ
ﮥﺷﻮﮔ رد ﻪﻧﻮﻤﻧ صﻮﺼﺨﻣ ﻞﺤﻣ ﻞﺧاد هﺪﺷ ﻪﯿﻬﺗ يﺎﻫ ﻪﻧﻮﻤﻧ ﻢﯾداد راﺮﻗ هﺎﮕﺘﺳد ﺖﺳار ﺖﻤﺳ .
ﺎﺗ هﺎﮕﺘﺳد رﻮﺘﯿﻧﺎﻣ ﻪﮑﻨﯾا زا ﺪﻌﺑ
4 ﺳﺪﺑ دﺪﻋ ﺪﺷ ﺖﺑﺎﺛ دﺪﻋ و دﺮﮐ ﺮﭘ ﻂﺧ ﺪﯾدﺮﮔ ﺖﺋاﺮﻗ هﺪﻣآ ﺖ
) هرﺎﻤﺷ ﻪﺑ ناﺮﯾا ﯽﻠﻣ دراﺪﻧﺎﺘﺳا ﻖﺑﺎﻄﻣ 608
( ] 5 [.
2 - 4 - 2 - نﺪﺷ زﺎﻓود ناﺰﯿﻣ ﻦﯿﯿﻌﺗ
ﻦﯿﯿﻌﺗ رﻮﻈﻨﻣ ﻪﺑ نﺪﺷ يزﺎﻓود
راﺪﻘﻣ 10 ﻪﻧﻮﻤﻧ زا مﺮﮔ تﻼﮑﺷ
هﺎﮕﺘﺳد رد و ﺪﺷ هداد راﺮﻗ جرﺪﻣ ﯽﻫﺎﮕﺸﯾﺎﻣزآ ﻪﻟﻮﻟ رد ﺖﻋﺮﺳ ﺎﺑ ژﻮﯿﻔﯾﺮﺘﻧﺎﺳ 5000rpm
تﺪﻣ ﻪﺑ 10 يﺎﻣد رد ﻪﻘﯿﻗد
ﻂﯿﺤﻣ ﺖﻓﺮﮔ راﺮﻗ ژﻮﯿﻔﯾﺮﺘﻧﺎﺳ ﺖﺤﺗ .
اﺪﺟ ﯽﺑآ زﺎﻓ ﻢﺠﺣ ﺲﭙﺳ
زا هﺪﺷ تﻼﮑﺷ ﯽﻠﯿﻣ ﺐﺴﺣ ﺮﺑ ، ﺪﯾدﺮﮔ ﺖﺒﺛ ﺮﺘﯿﻟ
] 13 [.
2 - 4 - 3 - هزاﺪﻧا ﻪﺘﯾزﻮﮑﺴﯾو يﺮﯿﮔ
ﻪﻧﻮﻤﻧ ﻪﺘﯾزﻮﮑﺴﯾو ﻦﯿﯿﻌﺗ ﺖﻬﺟ هﺎﮕﺘﺳد زا ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ يﺎﻫ
ﺮﺘﻣﻮﮑﺴﯾو Fungilab
ﺪﺷ هدﺎﻔﺘﺳا ﺎﯿﻧﺎﭙﺳا رﻮﺸﮐ ﺖﺧﺎﺳ .
اﺪﺘﺑا
200 ﯽﻠﯿﻣ د ار تﻼﮑﺷ زا ﺮﺘﯿﻟ رد ﻦﯾزﻮﺗ زا ﺪﻌﺑ و ﻪﺘﺨﯾر ﺮﺸﺑ ر
ﯽﻣ لﺎﯿﺳ يرﺎﻣ ﻦﺑ ﻢﯿﻨﮐ
. هرﺎﻤﺷ لﺪﻨﭙﺳا ﺎﺑ ﺲﭙﺳ 3
راﺪﻘﻣ هﺎﮕﺘﺳد
ﺪﺷ ﻦﯿﯿﻌﺗ ﺎﻫ ﻪﻧﻮﻤﻧ ﻪﺘﯾزﻮﮑﺴﯾو ]
14 [.
2 - 4 - 4 - ﯽﺘﻓﺎﺑ صاﻮﺧ
ﺞﻨﺳ ﺖﻓﺎﺑ هﺎﮕﺘﺳد زا ﺎﻫ ﻪﻧﻮﻤﻧ ﯽﺘﻓﺎﺑ يﺎﻫ ﯽﮔﮋﯾو ﻦﯿﯿﻌﺗ ياﺮﺑ
3
لﺪﻣ TAPluse ﺪﯾدﺮﮔ هدﺎﻔﺘﺳا
. تﻼﮑﺷ ﻪﻧﻮﻤﻧ زا ﻞﺒﻗ ﻪﻧﺎﺤﺒﺻ
3. Texture Analyzer
آ مﺎﺠﻧا تﺪﻣ ﻪﺑ نﻮﻣز 24
ﺪﺷ يراﺪﻬﮕﻧ لﺎﭽﺨﯾ رد ﺖﻋﺎﺳ .
ﺮﻄﻗ ﻪﺑ يا ﻪﻧاﻮﺘﺳا بوﺮﭘ زا ﯽﺘﻓﺎﺑ يﺎﻫ ﯽﮔﮋﯾو ﻦﯿﯿﻌﺗ ﺖﻬﺟ 45
ﻖﻤﻋ ﻪﺑ ذﻮﻔﻧ ياﺮﺑ ﺮﺘﻣ ﯽﻠﯿﻣ 20
ﺖﻋﺮﺳ ﺎﺑ ﺎﻫ ﻪﻧﻮﻤﻧ ﺮﺘﻣ ﯽﻠﯿﻣ 1
ﺪﺷ هدﺎﻔﺘﺳا ﻪﯿﻧﺎﺛ ﺮﺑﺮﺘﻣ ﯽﻠﯿﻣ .
صﻮﺼﺧ رد ﺖﻓﺎﺑ ﯽﺘﻔﺳ ﺮﺘﻣارﺎﭘ
ﻪﻧﻮﻤﻧ ﺖﻓﺮﮔ راﺮﻗ ﯽﺑﺎﯾزرا درﻮﻣ ﺎﻫ ]
15 [.
2 - 4 - 5 - ﺪﻧز يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ ه ﮏﯿﺗﻮﯿﺑوﺮﭘ
ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ داﺪﻌﺗ شرﺎﻤﺷ ياﺮﺑ
،تﻼﮑﺷ رد 10
مﺮﮔ زا
ﺎﺑ تﻼﮑﺷ 90 ml
تﺎﻔﺴﻓ ﺮﻓﺎﺑ ﻞﯾﺮﺘﺳا لﻮﻠﺤﻣ )
1M /0 و
7
= (pH ﻪﺑ تﺪﻣ 15 هﺪﻨﮐاﺮﭘ و ﺪﺷ هدز ﻢﻫ ﻪﻘﯿﻗد ﺎﺗ ﺖﺸﮔ
يﺮﺘﮐﺎﺑ و ﻪﺘﺴﮑﺷ ﺎﻫ لﻮﺴﭙﮐ دازآ ﺮﻓﺎﺑ لﻮﻠﺤﻣ رد ﻞﻣﺎﮐ رﻮﻃ ﻪﺑ
يﺎﻫ ﺖﻗر ﺲﭙﺳ ،ﺪﻧﻮﺷ
1
10- 11ﺎﺗ 10-
ﻂﯿﺤﻣ رد و هﺪﺷ هدﺎﻣآ
ﺖﺸﮐ MRS-agar يﺎﻣد رد
°C 37 ﻪﺑﻮﮑﻧا ﺪﯾدﺮﮔ . داﺪﻌﺗ
يﺮﺘﮐﺎﺑ ﺖﺷﺬﮔ زا ﺲﭘ هﺪﻧز 48
ﺮﺘﻧﺎﮐ ﯽﻨﻠﮐ هﺎﮕﺘﺳد ﺮﯾز ﺖﻋﺎﺳ
ﺪﯾدﺮﮔ شرﺎﻤﺷ .
2 - 5 - يرﺎﻣآ ﻞﯿﻠﺤﺗ و ﻪﯾﺰﺠﺗ
لﺪﻣ لﻮﺼﺣ رﻮﻈﻨﻣ ﻪﺑ ﺎﻫ
ي زا ماﺪﮐ ﺮﻫ ﯽﻨﯿﺑ ﺶﯿﭘ ياﺮﺑ ﯽﺑﺮﺠﺗ
ﺦﺳﺎﭘ ﺎﻫ ﯿﻓ ي
،ﯽﯾﺎﯿﻤﯿﺷﻮﮑﯾﺰ ﯽﺑوﺮﮑﯿﻣ و يژﻮﻟﻮﺋر
ﻪﻄﺑار ﺎﻫ ي
ﻪﻠﻤﺟ ﺪﻨﭼ و ﯽﻄﺧ ا
ي هداد ﺮﺑ مود ﻪﺟرد ﺎﻫ
ي زا هﺪﻣآ ﺖﺳد ﻪﺑ
ﺎﻣزآ ﺶﯾ ﺎﻫ ﺪﻧﺪﺷ شزاﺮﺑ .
لﺪﻣ ﻦﯾا ﺲﭙﺳ يرﺎﻣآ ﺰﯿﻟﺎﻧآ درﻮﻣ ﺎﻫ
ددﺮﮔ ﺶﻨﯾﺰﮔ ﺐﺳﺎﻨﻣ لﺪﻣ ﺎﺗ ﺪﻨﺘﻓﺮﮔ راﺮﻗ .
ﻐﺘﻣ ﯿﺮ درﻮﻣ يﺎﻫ
ﯽﺳرﺮﺑ ﺖﻈﻠﻏ ﻞﻣﺎﺷ ﯽﺑﺮﻋ ﻎﻤﺻ
، راﻮﮔ و ﻦﯿﺘﯿﺴﻟ هدوﺪﺤﻣ رد
ﭘ سﺎﺳا ﺮﺑ ﺐﺳﺎﻨﻣ ﯿ
ﺶ ﺗ ﯿ ﺎﻫرﺎﻤ ي مﺎﺠﻧا حﺮﻃ ﺐﻟﺎﻗ رد ،هﺪﺷ
ﺎﻣزآ ﯾ ﺸ ﯽ ﺢﻄﺳ - ﻪﺑ ﺦﺳﺎﭘ ترﻮﺻ Box-Behnken ﻌﺗ
ﯿﯿ ﻦ
ﺪﯾدﺮﮔ . حﺮﻃ ر ﯾﺰ ي ﺎﻣزآ ﯾ ﺶ
،ﺎﻫ لﺪﻣ زﺎﺳ ي ﺳﺮﮔر ﯿ ﻧﻮ ﯽ رﻮﮕﻟا ﺎﺑ ﯾﺘ ﻢ
ﺲﭘ فﺬﺣ ﻬﺑ ،رﻮﺧ
ﯿ ﻪﻨ زﺎﺳ ي دﺪﻋ ي ﺎﻫرادﻮﻤﻧ ﻢﺳر و ي
ﺢﻄﺳ
مﺮﻧ ﺎﺑ ﺦﺳﺎﭘ راﺰﻓا
Design-Expert مﺎﺠﻧا
ﺪﯾدﺮﮔ ) لوﺪﺟ 1 .(
ﻢﺳر مﺮﻧ ﺎﺑ تﺎﺒﺳﺎﺤﻣ و ﺎﻫرادﻮﻤﻧ ﺰﻓا
را Excel ترﻮﺻ ﺖﻓﺮﮔ .
نﻮﻣزآ ﻪﯿﻠﮐ رد ﺎﻫ
3 تﺪﻣ ﻪﺑ راﺮﮑﺗ 3
ﯽﻧﺎﻣز هزﺎﺑ و هﺎﻣ 2
ﻪﺘﻔﻫ يا
ﺪﯾدﺮﮔ مﺎﺠﻧا .
Table 1 Levels (percent) and amount (in grams per 100 grams of chocolate)
3 - ﺚﺤﺑ ﺞﯾﺎﺘﻧ
3 - 1 - ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ ﺮﯿﻐﺘﻣ ﺶﻨﮐ ﻢﻫﺮﺑ ﺮﯿﺛﺎﺗ يﺎﻫ ﻪﻧﻮﻤﻧ يزاﺪﻧا بآ و ﯽﺑآ ﺖﯿﻟﺎﻌﻓ يور ﺮﺑ
تﻼﮑﺷ مﺮﮐ
هداد يرﺎﻣآ ﻪﯾﺰﺠﺗ ﺞﯾﺎﺘﻧ راﻮﮔ يﺎﻫ ﻎﻤﺻ ،ﻦﯿﺘﺴﻟ ﺪﺻرد ﺮﺛا يﺎﻫ
ﻪﮐ ﺖﺳا نآ زا ﯽﮐﺎﺣ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ ﯽﺑآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ ﯽﺑﺮﻋ و ﻫ لﺪﻣ زا ﮏﯾ ﭻﯿﻫ ﯽﺑآ ﺖﯿﻟﺎﻌﻓ صﻮﺼﺧ رد ﯿﻧ راد ﯽﻨﻌﻣ ﺎ
و ﺖﺴ
ﺮﯿﻐﺘﻣ ،ﺮﮕﯾد ترﺎﺒﻋ ﻪﺑ دراﺪﻧ ﯽﺑآ ﺖﯿﻟﺎﻌﻓ ﺮﺑ يراد ﯽﻨﻌﻣ ﺮﯿﺛﺎﺗ ﺎﻫ
) 05 /0
>
.(P تﻼﮑﺷ يﺎﻫ ﻪﻧﻮﻤﻧ ياﺮﺑ ﯽﺑآ ﺖﯿﻟﺎﻌﻓ ﻂﺳﻮﺘﻣ
62 /0 ﺖﺳا هﺪﺷ شراﺰﮔ .
يزاﺪﻧا بآ ﻂﺳﻮﺘﻣ ﻪﻌﻟﺎﻄﻣ ﻦﯾا رد
ﯽﻣ ﺮﻈﻧ ﻪﺑ ﻪﮐ ﺪﺷ ﻪﺒﺳﺎﺤﻣ ﺮﻔﺻ تﻼﮑﺷ يﺎﻫ ﻪﻧﻮﻤﻧ ياﺮﺑ ﺪﺳر
ﻦﯾا ندوﺰﻓا ،ﺎﻫ ﻎﻤﺻ بآ بﺬﺟ ﯽﮔﮋﯾو ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ تﺎﺒﯿﮐﺮﺗ
ﮏﯾ ﭻﯿﻫ رد ﻪﺠﯿﺘﻧ رد و ﺖﺳا هﺪﯾدﺮﮔ يزاﺪﻧا بآ ﺮﺑ ﺮﯿﺛﺎﺗ ﺐﺒﺳ ﺪﺸﻧ هﺪﻫﺎﺸﻣ يزاﺪﻧا بآ ﺎﻫ ﻪﻧﻮﻤﻧ زا )
05 /0
>
.(P
3 - 2 - ﯽﺳرﺮﺑ درﻮﻣ يﺎﻫ ﺮﯿﻐﺘﻣ ﺶﻨﮐ ﻢﻫﺮﺑ ﺮﯿﺛﺎﺗ
ﺘﻔﺳ ﺮﺑ ﯽ ﺖﻓﺎﺑ
ﺎﺘﻧ ﯾ ﺞ ﺰﺠﺗ ﯾﻪ رﺎﻣآ ي هداد ﺎﻫ ي ﺘﺴﻟ ﺪﺻرد ﺮﺛا ﯿ
،ﻦ ﺎﻫ ﻎﻤﺻ ي راﻮﮔ
ﺑﺮﻋ و ﯽ ﻐﺗ ﺮﺑ ﯿﯿ تاﺮ ﺘﻔﺳ ﯽ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ ﺖﻓﺎﺑ رد
ﻞﮑﺷ ) 1
و a (b ، ﺖﺳا هﺪﺷ هدروآ .
حﻮﻄﺳ مﺎﻤﺗ رد ﯽﻠﮐ رﻮﻃ ﻪﺑ
ﻦﯿﺘﯿﺴﻟ و راﻮﮔ ﻎﻤﺻ ﺪﺻرد ﺶﯾاﺰﻓا ﺎﺑ ﯽﺘﺨﺳ ﯽﻨﯾﺰﮕﯾﺎﺟ ﺖﻓﺎﯾ ﺶﯾاﺰﻓا )
05 /0 p<
( ﻻﺎﻤﺘﺣا ﺪﻧاﻮﺗ ﯽﻣ هﺪﯾﺪﭘ ﻦﯾا ﺖﻠﻋ ﻪﮐ
ﯽﻟژ ﻪﮑﺒﺷ ﻞﯿﮑﺸﺗ و هدﺎﻔﺘﺳا درﻮﻣ ﻎﻤﺻ ﻂﺳﻮﺗ ﻦﻏور بﺬﺟ ﺮﺑ ﺎﻫ ﻪﻧﻮﻤﻧ مﺎﮑﺤﺘﺳا ﺶﯾاﺰﻓا و ﺎﻫ ﺪﯿﺋﻮﻠﮐورﺪﯿﻫ ﻂﺳﻮﺗ ﺮﺛا
ﯽﺑﺮﻋ ﻎﻤﺻ ندوﺰﻓا ﻪﮐ ﯽﻟﺎﺣرد ،ﺪﺷﺎﺑ ﻪﮑﺒﺷ ﻦﯾا ﻞﯿﮑﺸﺗ ﺮﺑ
ﯽﻨﻌﻣ ﯽﺘﻔﺳ ﺮﺘﻣارﺎﭘ ﺖﺳا هدﻮﺒﻧ راد
. ناﺰﯿﻣ ﻦﯾﺮﺘﺸﯿﺑ ﻪﮐ يرﻮﻃ ﻪﺑ
يوﺎﺣ ﻪﻧﻮﻤﻧ رد ﯽﺘﻔﺳ 8/
0 زا ﺮﺘﻤﮐ و راﻮﮔ ﺪﺻرد 1/
0 ﺪﺻرد
ﺖﺳا هﺪﻣآ ﺖﺳد ﻪﺑ ﯽﺑﺮﻋ ﻎﻤﺻ .
ﺖﮑﺑ ) 2009 ( شراﺰﮔ داد ،نﻮﯿﺳﻻﻮﻣﺮﻓ ﺮﯿﻈﻧ يدﺪﻌﺘﻣ ﻞﻣاﻮﻋ ﻪﮐ
،ﺪﯿﻟﻮﺗ هﻮﺤﻧ ،ندﺮﮐ دﺮﺳ يﺎﻣد و ﻢﺴﯿﻓرﻮﻣ ﯽﻠﭘ ،ﮓﻨﯾﺮﭙﻤﺗ هﻮﺤﻧ
ﺖﺳا تﻼﮑﺷ يﺎﻫ ﻪﻧﻮﻤﻧ ﯽﯾﺎﻬﻧ ﯽﺘﺨﺳ هﺪﻨﻨﮐ ﻦﯿﯿﻌﺗ ]
3 [.
Run Lecithin guar arabic
gum Hanny Tahini Grape juice
Grape
juice Rose water Carda
mon vanilla Coconut powder
1 4.5 3 1.5 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
2 3 4.8 1.5 147.7 236.3 147.7 11.8 31.9 0.3 0.6 11.8
3 3 1.2 1.5 148.6 237.7 148.6 11.٩ 32.1 0.3 0.6 11.9
4 4.5 3 1.5 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
5 4.5 3 1.5 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
6 6 4.8 1.5 147.9 235.1 147.9 11.8 31.7 0.3 0.6 11.8
7 4.5 4.8 1.5 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
8 4.5 3 0 147.7 236.3 147.8 11.8 31.9 0.3 0.6 11.8
9
3 3 0 147.5 237.6 147.8 11.9 32.1 0.3 0.6 11.8
10 6 3 3 147.0 235.2 147.0 11.8 31.8 2.9 0.6 11.8
11 4.5 3 1.5 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
12 4.5 3 3 146.9 236.1 146.9 11.8 31.7 0.3 0.6 11.8
13 6 3 0 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
14 4.5 1.2 3 147.8 236.5 147.8 11.8 31.9 0.3 0.6 11.8
15 6 1.2 1.5 147.8 236.5 147.8 11.8 31.9 0.3 0.6 11.8
16 0 0 0 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
17 3 3 3 147.8 236.4 147.8 11.8 31.9 0.3 0.6 11.8
18 4.5 1.2 0 147.6 236.7 148.6 11.9 32.1 0.3 0.6 11.9
ﻪﺑ زﺎﯿﻧ مﺪﻋ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻪﮐ ﺖﻓﺮﮔ ﻪﺠﯿﺘﻧ ناﻮﺗ ﯽﻣ ﻦﯾاﺮﺑﺎﻨﺑ نﻮﻣزآ يﺎﻣد و ﺎﻫ ﻪﻧﻮﻤﻧ ندﺮﮐ دﺮﺳ يﺎﻣد ندﻮﺑ ﺖﺑﺎﺛ ،ﮓﻨﯾﺮﭙﻤﺗ ﺶﯾاﺰﻓا ﺶﻫوﮋﭘ ﻦﯾا يﺎﻫ ﻪﻧﻮﻤﻧ ﻪﯿﻠﮐ ياﺮﺑ ﯽﺘﺨﺳ ﯽﺘﻔﺳ ﺪﺻرد
ﺮﺑ راﻮﮔ ﻎﻤﺻ ندوﺰﻓا ﺎﺑ ﺮﺑ نآ ﺮﯿﺛﺎﺗ و ﻎﻤﺻ يﺎﻫ ﯽﮔﮋﯾو
ﺪﺷﺎﺑ ﻂﺒﺗﺮﻣ هﺪﺷ ﻪﯿﻬﺗ تﻼﮑﺷ رﺎﺘﺧﺎﺳوﺮﮑﯿﻣ ]
3 [.
Design-Expert® Software Factor Coding: Actual Hardness (N)
Design points above predicted value Design points below predicted value 8.18587
2.5583 X1 = B: Guar Gum X2 = C: Arabic Gum Actual Factor A: Lecithin = 0.75
0 0.1 0.2 0.3 0.4 0.5 0.2 0.3 0.4 0.5 0.6 0.7 0.8
2 3 4 5 6 7 8 9
Hardness (N)
B: Guar Gum (%)
C: Arabic Gum (%)
Design-Expert® Software Factor Coding: Actual Hardness (N)
Design points above predicted value Design points below predicted value 8.18587
2.5583 X1 = A: Lecithin X2 = B: Guar Gum Actual Factor C: Arabic Gum = 0.25
0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.5
0.6 0.7
0.8 0.9
1 2
3 4 5 6 7 8 9
Hardness (N)
A: Lecithin (%)
B: Guar Gum (%)
Fig 1 Interaction of a) arabic and guar gum b) lecithin and guar gum on the of hardness of
breakfast chocolate
3 - 3 - يﺎﻫ ﺮﯿﻐﺘﻣ ﺶﻨﮐ ﻢﻫﺮﺑ ﺮﯿﺛﺎﺗ ﯽﺳرﺮﺑ درﻮﻣ
ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ ﻪﺘﯾزﻮﮑﺴﯾو ﺮﺑ
نﺎﻤﻫ ﻞﮑﺷ رد ﻪﮐ رﻮﻃ )
3 و a (b لوﺪﺟ و 2 هﺪﺷ هداد نﺎﺸﻧ ،
،ﺖﺳا ﮐ ندوﺰﻓا ﻨﻌﻣ ﺮﯿﺛﺄﺗ راﻮﮔ و ﯽﺑﺮﻋ ﻎﻤﺻ ﺲﮑﻠﭙﻤ ﯽ
ﺮﺑ يراد
ﺖﺷاﺪﻧ ﯽﺗﻼﮑﺷ مﺮﮐ ﻪﺘﯾزﻮﮑﺴﯾو )
05 /0
≥ (،p ﯽﻟﺎﺣرد ﻪﮐ
ﺮﺑ ﻦﯿﺘﯿﺴﻟ ﺮﯾﺎﻔﯿﺴﻟﻮﻣا و راﻮﮔ ﻎﻤﺻ ﺲﮑﻠﭙﻤﮐ ندوﺰﻓا ﯽﻨﻌﻣ ﻪﺘﯾزﻮﮑﺴﯾو راد
ﺖﺳا هدﻮﺑ )
05 /0
≤ .(p ﺶﯾاﺰﻓا ﺎﺑ ﻪﮑﯾرﻮﻄﺑ هدوﺪﺤﻣ ﺎﺗ راﻮﮔ ﻎﻤﺻ ﺮﯾدﺎﻘﻣ
8/
0 ﺎﺗ ﺮﯾﺎﻔﯿﺴﻟﻮﻣا ﺶﻫﺎﮐ و 5/
0
ﯽﻨﻌﻣ ﺪﻧور ﺪﯾدﺮﮔ هﺪﻫﺎﺸﻣ يراد
. راﻮﮔ ﻎﻤﺻ ﺮﯿﺛﺎﺗ ،نﺎﯿﻣ ﻦﯾا رد
لﻮﺼﺤﻣ ﻪﺘﯾزﻮﮑﺴﯾو ﺶﯾاﺰﻓا رد ار يﺮﺘﻬﺑ دﺮﮑﻠﻤﻋ ﯽﯾﺎﻬﻨﺗ ﻪﺑ دﻮﻤﻧ ﺎﻔﯾا ﯽﯾﺎﻬﻧ .
ا يﺮﯿﮔﻮﻠﺟ رد ﺎﻫﺪﯿﺋﻮﻠﮐورﺪﯿﻫ ﻞﻤﻋ مﺰﯿﻧﺎﮑﻣ ز
هدﺎﻔﺘﺳا درﻮﻣ ﺪﯿﺋﻮﻠﮐورﺪﯿﻫ ﯽﻟﻮﮑﻟﻮﻣ رﺎﺘﺧﺎﺳ ﻪﺑ مﺮﺳ نﺪﺷ اﺪﺟ زا ﺪﺷﺎﺑ رادرﺎﺑ هدﺎﻔﺘﺳا درﻮﻣ ﻎﻤﺻ ﻪﮐ ﯽﺗرﻮﺻ رد دراد ﯽﮕﺘﺴﺑ ﺎﭘ ﻊﻓد و ﯽﯾﺎﻀﻓ ﺖﻌﻧﺎﻤﻣ ﻖﯾﺮﻃ ﺲﮑﻠﭙﻤﮐ رد يراﺪﯾ
ﯽﯾاﺬﻏ يﺎﻫ
ﻂﺳﻮﺗ ﯽﻣ راﻮﮔ ﻎﻤﺻ ﻦﺘﺧاﺪﻧا ماد ﻪﺑ و ﻪﺘﯾزﻮﮑﺴﯾو ﺶﯾاﺰﻓا دﻮﺷ
ﺗ ﻪﮐ ﯽﻟﻮﮑﻟﻮﻣ ﻪﮑﺒﺷ ﮏﯾ رد ﯽﻨﯿﺌﺗوﺮﭘ تارذ دﺎﺠﯾا ﻎﻤﺻ ﻂﺳﻮ
ﯽﻣ هﺪﺷ ﺪﺷﺎﺑ ] 16 [ . ﺪﻤﺣا ﺶﻫوﮋﭘ زا ﻞﺻﺎﺣ ﺞﯾﺎﺘﻧ )
2006 ( ﺮﺑ
داد نﺎﺸﻧ يﺮﯿﻤﺨﺗ تﻻﻮﺼﺤﻣ رد راﻮﮔ ﻎﻤﺻ تﺎﯿﺻﻮﺼﺧ يور ﮏﯾژﻮﻟﻮﺋر رﺎﺘﻓر ﺮﯿﯿﻐﺗ ﺐﺒﺳ ﯽﯾﺎﻬﻨﺗ ﻪﺑ راﻮﮔ ﻎﻤﺻ يﺮﯿﮔرﺎﮑﺑ ﻪﮐ تﻻﻮﺼﺤﻣ رد يزﺎﻓ يزﺎﺳاﺪﺟ ﺶﯾاﺰﻓا و يوﺮﻧاﺮﮔ ﺶﻫﺎﮐ و ﯽﻣ ﯽﻨﺒﻟ ﻪﺑﺎﭘ ﺺﺧﻻﺎﺑ ﯽﯾاﺬﻏ ددﺮﮔ
] 17 [.
Fig 2 effect of adetive Arabic, guar gum and lecithin of viscosity breakfast chocol
a)
a)
b)
b)
Analysis of variability of viscosity of breakfast chocolate samples Table 2
3 - 4 - و راﻮﮔ ،ﯽﺑﺮﻋ يﺎﻫ ﻎﻤﺻ ﺮﺛا ﯽﺳرﺮﺑ
ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز ﺮﺑ ﻦﯿﺘﯿﺴﻟ
يﺎﻫ ﻪﻧﻮﻤﻧ مﺮﮐ
وﺎﺣ تﻼﮑﺷ يﺮﺘﮐﺎﺑ نﺎﺴﮑﯾ ﺮﯾدﺎﻘﻣ ي
ﮏﯿﺗﻮﯿﺑوﺮﭘ يراﺪﻬﮕﻧ هﺎﻣ ﻪﺳ ﯽﻃ ،
هﺪﻧز ناﺰﯿﻣ هﺎﻣ ﺮﻫ ﯽﻃ و ﺪﺷ
داﺪﻌﺗ ﻪﺑ ﺖﺒﺴﻧ لﺎﻌﻓ و هﺪﻧز يﺎﻫ يﺮﺘﮐﺎﺑ ﺪﺻرد ﯽﻨﻌﯾ ،ﯽﻧﺎﻣ نآ ﺖﻓﺮﮔ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ،لوا زور رد ﺎﻫ .
،ﻞﺻﺎﺣ ﺞﯾﺎﺘﻧ ياﺮﺑ
ار لﺪﻣ شزاﺮﺑ و يرﺎﻣآ ﺰﯿﻟﺎﻧآ ﺖﯿﻠﺑﺎﻗ لوا هﺎﻣ يﺎﻫ هداد ﺎﻬﻨﺗ ﻞﺑﺎﻗ ﺶﻫﺎﮐ ﻞﯿﻟد ﻪﺑ ،مﻮﺳ و مود يﺎﻫ هﺎﻣ يﺎﻫ هداد و ﺖﺷاد داﺪﻌﺗ ﻪﺟﻮﺗ ﻪﻧﻮﻤﻧ ﻪﻤﻫ رد و ﻪﯾﻮﺳ ود ﺮﻫ رد هﺪﻧز يﺎﻫ يﺮﺘﮐﺎﺑ
ﺖﻓﺮﮕﻧ راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ ،ﺎﻫ .
ﺰﯿﻟﺎﻧآ ،سﺎﺳا ﻦﯾا ﺮﺑ مﺎﺠﻧا يﺎﻫ
ارا يراﺪﻬﮕﻧ هﺎﻣ ﮏﯾ ﯽﻃ ﯽﻧﺎﻣ هﺪﻧز ياﺮﺑ هﺪﺷ ﺋ
ﻪ ﺪﯾدﺮﮔ .
هداد يرﺎﻣآ ﻪﯾﺰﺠﺗ ﺞﯾﺎﺘﻧ د ﺮﺛا يﺎﻫ
راﻮﮔ يﺎﻫ ﻎﻤﺻ ،ﻦﯿﺘﺴﻟ ﺪﺻر
ﯽﺑﺮﻋ و ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز ﺮﺑ مﺮﮐ
ﺻ تﻼﮑﺷ ﺒ
ﻪﻧﺎﺤ
رد يرﺎﮔﺪﻧﺎﻣ هﺎﻣ ﮏﯾ ﯽﻃ ﻞﮑﺷ
) 4 ( ، ﺖﺳا هﺪﺷ هدروآ .
نﺎﻤﻫ ﻞﮑﺷ رد ﻪﮐ رﻮﻃ )
4 (،
،دﻮﺷ ﯽﻣ ﻪﻈﺣﻼﻣ ﺎﺑ
ﺶﯾاﺰﻓا ﺢﻄﺳ
راﻮﮔ ﻎﻤﺻ و ﻦﯿﺘﺴﯿﻟ ناﺰﯿﻣ
ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز
ﻻ ﺶﯾاﺰﻓا مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎﺑﻮﺘﮐ ﺖﻓﺎﯾ
ناﺰﯿﻣ ندوﺰﻓا ﻪﮐ ﯽﻟﺎﺣرد
ﯽﺑﺮﻋ ﻎﻤﺻ ﺶﯿﺑ ﻪﺑ
زا 3/
0 ﻧز ﺶﻫﺎﮐ ﺐﺒﺳ ﺪﺻرد ﺎﻣ هﺪ
ﯽﻧ
دﺮﮔ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﺪﯾ
) 05 /0 p>
.(
رﺎﺘﺧﺎﺳ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﻦﯾا ﺪﺻرد ﺶﯾاﺰﻓا ﻻﺎﻤﺘﺣا ﯽﺑﺮﻋ ﻎﻤﺻ هﺪﯿﭽﯿﭘ و ﺐﻌﺸﻨﻣ ناﺰﯿﻣ ﺶﻫﺎﮐ و هﺪﯾدﺮﮔ ﻪﻧﻮﻤﻧ بآ بﺬﺟ ﺶﯾاﺰﻓا ﺐﺒﺳ ﺐﯿﮐﺮﺗ aw
ﺖﺳا هﺪﯾدﺮﮔ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﺎﻘﺑ و ﺪﺷر ﺶﻫﺎﮐ ﺐﺒﺳ .
Fig 3 effect of adetive Arabic, guar gum and Lisetin on the survival of the probiotic bacteria (L.
Pelantarum) breakfast chocol during one month of shelf life
ﺮﺘﯿﻟ ﯽﻠﯿﻣ ﺮﻫ رد نﻮﯿﻠﯿﻣ ﮏﯾ يﺮﺘﮐﺎﺑ داﺪﻌﺗ ﻞﻗاﺪﺣ ياﺮﺑ
ﮏﯿﺗﻮﯿﺑوﺮﭘ تﻻﻮﺼﺤﻣ ﺖﺳا هﺪﺷ ﻒﯾﺮﻌﺗ دراﺪﻧﺎﺘﺳا ﺪﺣ رد
) 8 .(
ﯽﻣ نﺎﺸﻧ ﺞﯾﺎﺘﻧ ﺖﻇﺎﻔﺣ ﺎﺑ ﺪﻧاﻮﺗ ﯽﻣ راﻮﮔ ﻎﻤﺻ ندوﺰﻓا ﻪﮐ ﺪﻫد
ﺪﻨﮐ ﺖﯾﻮﻘﺗ ار ﺎﻫ نآ ﯽﻧﺎﻣ هﺪﻧز ﺎﻫ يﺮﺘﮐﺎﺑ زا .
ﺶﻘﻧ ﻻﺎﻤﺘﺣا
ﺮﭘ ﺐﯿﮐﺮﺗ ﮏﯾ ناﻮﻨﻋ ﻪﺑ هﺪﺷ هدوﺰﻓا ﻎﻤﺻ و
ﻪﺑ ردﺎﻗ ﮏﯿﺗﻮﯿﺑ
زا ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺎﻫ يﺮﺘﮐﺎﺑ ﻆﻔﺣ ﺐﯿﺳآ
يﺎﻫ ﻦﻣ ﯽﻄﯿﺤﻣ ﻠﻤﺟ
ﻪ
و يﺰﻤﺳا رﺎﺸﻓ ،نﮋﯿﺴﮐا ...
رد ار دﻮﺧ ﺪﯿﻔﻣ تاﺮﺛا و ﺪﺷﺎﺑ ﯽﻣ
Source Df
average of squares sum of
squares F Value
P Value
Width of origin 6
5.45
23.34
23.37
0.00001
<
Significant
A-Lecithin
1 6.91
6.13
36.84
0.00001
C-Arabic Gum
1 5.17
٦
. ٣ 21.62
0.0009
AC 1
0.045
004
- E 5.372 003 - E 3.227 0.9558
Residual
1 12.50
7.99
47.97
0.00001
<
Lack of Fit
1 2.78
1.86
11.16
0.0075
not significant
Pure Error
1 5.71
4.05
Cor Total
10
R2 6
(R2) corrected
4
Coefficient of variation 16
b) a)
ﻔﺣ ﺪﻫد ﯽﻣ نﺎﺸﻧ ﺎﻫ يﺮﺘﮐﺎﺑ ﺪﺷر ﺖﯾﻮﻘﺗ و ﻆ .
هﺪﺷ شراﺰﮔ
رد ﺪﻣﺎﺟ ﻪﻤﯿﻧ يﺎﻫ هدرواﺮﻓ يا ﻪﮑﺒﺷ رﺎﺘﺧﺎﺳ ﺰﯾر ﻪﮐ ﺖﺳا ﺑ ﻪﺴﯾﺎﻘﻣ هدرواﺮﻓ ﻦﯾا يا هرذ رﺎﺘﺧﺎﺳ ﺰﯾر ﺎ ﻊﯾﺎﻣ ﯽﯾاﺬﻏ داﻮﻣ ﺎﺑ ﺎﻫ
ﺮﺛا ﺎﻧ ﻞﻣاﻮﻋ ﻞﺑﺎﻘﻣ رد يﺮﺘﻬﺑ ﯽﺘﻇﺎﻔﺣ و ﻪﺘﯾﺪﯿﺳا ﻪﻠﻤﺟ زا ﺐﺳﺎﻨﻣ
دراد ﯽﺑوﺮﮑﯿﻣ ﺪﺿ تﺎﺒﯿﮐﺮﺗ ﺮﯾﺎﺳ .
ﻦﯾا ﺖﻠﻋ ﺖﻌﻧﺎﻤﻣ عﻮﺿﻮﻣ
ﺪﺿ تﺎﺒﯿﮐﺮﺗ دورو زا ﯽﯾاﺬﻏ هدﺎﻣ ﺪﻣﺎﺟ ﻪﻤﯿﻧ ﻪﮑﺒﺷ ﯽﮑﯾﺰﯿﻓ ﺪﯿﻔﻣ يﺎﻫ يﺮﺘﮐﺎﺑ ﺎﺑ نﺪﺷ روﺎﺠﻣ و ﻪﮑﺒﺷ نورد ﻪﺑ ﯽﺑوﺮﮑﯿﻣ ﺖﺳا نآ رد دﻮﺟﻮﻣ ]6
و 11 [.
ﯽﺘﺴﺒﻧ نارﺎﮑﻤﻫ و )
2012 ( ﺮﯿﺛﺎﺗ يﺪﯿﻠﮐ ﻞﻣاﻮﻋ ﺪﻧدﺮﮐ شراﺰﮔ
يﺎﻫ هدرواﺮﻓ رد ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺎﻫ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز ﺮﺑ راﺬﮔ
،يدﺎﻨﻗ ﺖﺳا ﺎﻣد و يﺰﻤﺳا رﺎﺸﻓ ،ﯽﺑآ ﺖﯿﻟﺎﻌﻓ ]
18 [.
ﺴﺒﻧ ﯽﺘ و
نارﺎﮑﻤﻫ ) 2005 ( ﯽﺋزﺎﮐ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ يﺎﻫ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز
يوﺎﺣ تﻼﮑﺷ يﺎﻫ ﻪﻧﻮﻤﻧ رد ار ﯽﺋزﺎﮐارﺎﭘ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ و يﺎﻫﺎﻣد رد يﺮﻟﺎﮐ ﻢﮐ يﺎﻫ ﺪﻨﻗ ﯽﺧﺮﺑ و زرﺎﮐﺎﺳ 4
، 18 و 30
تﺪﻣ ﻪﺑ داﺮﮕﯿﺘﻧﺎﺳ ﻪﺟرد 12
ﻧداد راﺮﻗ ﯽﺳرﺮﺑ درﻮﻣ هﺎﻣ ﻪﺑ و ﺪ
دﻮﺧ ﯽﻧﺎﻣ هﺪﻧز ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺎﻫ يﺮﺘﮐﺎﺑ ﻪﮐ ﺪﻧﺪﯿﺳر ﻪﺠﯿﺘﻧ ﻦﯾا ﺪﻨﭼ ﺮﻫ ﺪﻧدﺮﮐ ﻆﻔﺣ ﯽﯾﻻﺎﺑ يﺎﻫ ﺪﺻرد ﺎﺗ ﺎﻫ ﻪﻧﻮﻤﻧ ﻪﯿﻠﮐ رد ار يﺎﻣد رد ﺎﻫ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز 4
دﻮﺑ ﺮﺘﺸﯿﺑ داﺮﮕﯿﺘﻧﺎﺳ ﻪﺟرد ]
1 [.
3 - 5 - ﻪﻨﯿﻬﺑ يزﺎﺳ
ﻪﺟﻮﺗ ﺎﺑ ﺑ ﻪ ﮏﯾ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻪﮐ ﻪﺘﮑﻧ ﻦﯾا و ﺎﻫرادﻮﻤﻧ ﻞﯿﻠﺤﺗ
ﻤﻣ ،ﺦﺳﺎﭘ ﻦﯾاﺮﺑﺎﻨﺑ ،ﺪﺷﺎﺑ ﺐﺳﺎﻨﻣﺎﻧ ﺮﮕﯾد ﺦﺳﺎﭘ ياﺮﺑ ﺖﺳا ﻦﮑ
ﺦﺳﺎﭘ ﯽﻣﺎﻤﺗ نﺎﮑﻣا ﺪﺣ ﺎﺗ ﻪﮐ دﺮﮐ ﯽﻓﺮﻌﻣ ار يﻮﮕﻟا ﯽﺘﺴﯾﺎﺑ ار ﺎﻫ
ﺪﯾﺎﻤﻧ ﻪﻨﯿﻬﺑ ﯽﺸﺨﺑ ﺖﯾﺎﺿر ﻮﺤﻧ ﻪﺑ .
رﻮﺘﻧﺎﮐ رﻮﻈﻨﻣ ﻦﯾا ياﺮﺑ
تﻼﭘ ﻪﻘﻄﻨﻣ و ﻪﺘﻓﺮﮔ راﺮﻗ ﻢﻫ يور ﺮﺑ ﻒﻠﺘﺨﻣ يﺎﻫ ﻪﮐ يا
ﺦﺳﺎﭘ ﯽﻣﺎﻤﺗ تﺎﺼﺨﺸﻣ ﻘﻄﻨﻣ ناﻮﻨﻋ ﻪﺑ ،دﺮﮐ هدروآﺮﺑ ار ﺎﻫ
ﻪﻨﯿﻬﺑ ﻪ
ﯽﻣ ﯽﻓﺮﻌﻣ ددﺮﮔ
. تﻼﮑﺷ ﺪﯿﻟﻮﺗ ياﺮﺑ ﻪﻨﯿﻬﺑ ﻂﯾاﺮﺷ ﻦﯾاﺮﺑﺎﻨﺑ
ﺖﻈﻠﻏ رد ﻪﻧﺎﺤﺒﺻ 564
/0 ،ﻦﯿﺘﺴﯿﻟ 8/
0 و راﻮﮔ 064 /0 ﻎﻤﺻ
هﺪﻧز ﻦﯾﺮﺘﺸﯿﺑ ﯽﺑﺮﻋ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ
cfu/gr 589 / 10 و
ﺖﻓﺎﺑ ﯽﺘﻔﺳ 22
/8 ﺪﺷ ﻢﻫاﺮﻓ ﻦﺗﻮﯿﻧ .
4 - يﺮﯿﮔ ﻪﺠﯿﺘﻧ
درواﺮﻓ ﺪﯿﻟﻮﺗ ﻪﺑ نﺎﮔﺪﻨﻨﮐ فﺮﺼﻣ زﺎﯿﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯽﯾاﺬﻏ يﺎﻫ ه
،ﯽﺑﺮﻋ يﺎﻫ ﻎﻤﺻ ندوﺰﻓا ﺮﯿﺛﺎﺗ ﺶﻫوﮋﭘ ﻦﯾا رد ﺶﺨﺑ ﯽﺘﻣﻼﺳ يﺎﻫ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز و ﯽﻔﯿﮐ يﺎﻫ ﯽﮔﮋﯾو ﺮﺑ ﻦﯿﺘﯿﺴﻟ و راﻮﮔ رد ﮏﯿﺗﻮﯿﺑوﺮﭘ مﺮﮐ
ﯽﺳرﺮﺑ درﻮﻣ ﮏﯿﺗﻮﯿﺑوﺮﭘ ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ
ﺖﻓﺮﮔ راﺮﻗ .
ﻎﻤﺻ ندوﺰﻓا ﻪﮐ دﻮﺑ نآ زا ﯽﮐﺎﺣ ﺶﻫوﮋﭘ ﺞﯾﺎﺘﻧ ﺎﺑ يﺎﻫ ﯽﮔﮋﯾو ﺮﺑ ﻦﯿﺘﯿﺴﻟ و راﻮﮔ
ﯽﺘﻓ ) ﺳ و ﺖﻓﺎﺑ ﯽﺘﻔ
ﻪﺘﯾزﻮﮑﺴﯾو (
ﯽﻨﻌﻣ دﻮﺑ راد ) 05 /0 p<
.(
ﯽﻟﺎﺣرد ﻪﮐ ﻎﻤﺻ ندوﺰﻓا
يرادﺎﻨﻌﻣ ﺮﯿﺛﺎﺗ ﯽﺑﺮﻋ ﺖﺷاﺪﻧ ﻪﺘﯾزﻮﮑﺴﯾو و ﺖﻓﺎﺑ ﯽﺘﻔﺳ ﺮﺑ
) 05 /0 p>
.(
يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز ﺮﺑ يرادﺎﻨﻌﻣ ﺮﯿﺛﺎﺗ راﻮﮔ ﻎﻤﺻ ندوﺰﻓا ﺖﺷاد مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ ﮏﯿﺗﻮﯿﺑوﺮﭘ )
05 /0 p<
( و
ﺎﺗ ﯽﺑﺮﻋ ﻎﻤﺻ ندوﺰﻓا 3/
0 ﺳ ﺪﺻرد هﺪﻧز ﯽﻧﺎﻣ هﺪﻧز ﺶﯾاﺰﻓا ﺐﺒ
ﺪﯾدﺮﮔ مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎﺑﻮﺘﮐﻻ ﮏﯿﺗﻮﯿﺑوﺮﭘ يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ ﺖﺳا ه
ﯽﻨﻌﻣ ﺶﯾاﺰﻓا ﻦﯾا لﺎﺣ ﻦﯿﻋ رد دﻮﺒﻧ راد
) 05 /0 p>
.(
ﺮﺛا ﻦﯿﻨﭽﻤﻫ
نﻮﯿﺳﻻﻮﻣﺮﻓ رد ﻦﯿﺘﯿﺴﻟ و ﯽﺑﺮﻋ ﻎﻤﺻ نﺎﻣﺰﻤﻫ ندوﺰﻓا مﺮﮐ
ﻪﻧﺎﺤﺒﺻ تﻼﮑﺷ ﺰﯿﻧ
ﺶﯾاﺰﻓا ﯽﻨﻌﻣ يراد يﺮﺘﮐﺎﺑ ﯽﻧﺎﻣ هﺪﻧز رد
ﺘﮐﻻ ﮏﯿﺗﻮﯿﺑوﺮﭘ مورﺎﺘﻧﻼﭘ سﻮﻠﯿﺳﺎﺑﻮ
ﺖﺷاﺪﻧ ) 05 /0 p>
(.
5 - ﻊﺑﺎﻨﻣ
[1] Nebesny E, Zyzelewicz, D. Effect of lecithin concentration on properties of sucrose-free chocolate masses sweetened with isomalt: Eur Food Res Technol 2005;
220: 131–135.
[2] Haylock SJ, Dodds TM. Ingredients from milk. In S. T. Beckett (Ed.), Industrial chocolate manufacture and use (3rd ed.):
Oxford: Blackwell Science1999; pp. 137- 152.
[3] Beckett, ST. The science of chocolate.
Edition 2, Royal Society of Chemistry Paperbacks 2009; 978-1-78262-595-7. P 223-233.
[4] Maghsoudi, SH. Candy and Chocolate Making Technology: Agricultural Science Publishing 2002; pp. 50-36.
[5] Iranian National Standard, No. 608, Chocolate - Characteristics and Test Methods (2004).
[6] Soukoulis C, Behboudi-Jobbehdar S, Yonekura L, Parmenter C. and Fisk I.
Stability of Lactobacillus rhamnosus GG in prebiotic edible films: Food Chemistry 2014a; 159,302–308.
[7] Soukoulis C, Yonekura L, Gan HH, Behboudi-Jobbehdar S, Parmenter C, and Fisk I. Probiotic edible films as a new strategy for developing functional bakery products: The case of pan bread, Food Hydrocolloids 2014b; 39,231–242.
[8] Ebrahimi B, Mohammadi R, Mortazavian SAM, and Shojaei Aliabadi S. Addition of
Probiotic Bacteria to Oral Film Based on Carboxymethyl Cellulose and Measurement of Survival Rate at Refrigeration and Ambient Temperatures: Journal of Food Industry Engineering Research 2017;
Volume 17, Number 65, pp. 90-79.
[9] Creel, RE. Effect of acacia gum on postharvest quality of cut flowers: Master of Science Thesis, Auburn, Alabama University 2006; 70. P.
[10] Fatemi H. Food Chemistry, Joint Stock Company, Tehran Publications. Pages 260- 259. 2004.
[11] Khairkhahan N, Ahri H, Asadi, Gh.
Feasibility study of producing probiotic chocolates containing Bifidobacterium breve bacterium microcoated with calcium alginate and resistant corn starch by emulsion method and evaluation of its viability: Veterinary microbiology 2017;57-73.
[12] Mandal SS, Hati AK, Puniya R, Singh and Singh. Development of Synbiotic Milk Chocolate using Encapsulated Lactobacillus Casei NCDC 298: Journal of Food Processing and Preservation ISSN 2012;
1745-4549.
[13] Prakash S, Huppertz T, Karvchuk O and Deeth H. Ultra-high-temperature processing of chocolate flavoured milk: Journal of Food Engineering 2010; 96: 179–184.
[14] Khorasani S, Azizi MH, Ishaqi, M R.
Investigation and determination of rheological properties of breakfast chocolate prepared from pistachios and dates: 23 National Congress of Food Science and Industry of Iran 2014.NCFOODI 23-431.
[15] Farzanmehr H, Abbasi S, Sahari MA.
Effect of sugar replacer on some physicochemical, rheological and sensory properties of milk chocolate: Iranian J Nutr Sci Food Technol 2008; 3(3):65–82[in Persian].
[16] Koksoy A, & Kilic M. Use of hydrocolloids in textural stabilization of a
yoghurt drink, ayran: Food
hydrocolloids2004; 18(4): 593-600.
[17] Ahmed FE. Genetically Modified Probiotics. In: Probiotics in Food Safety and Human Health. Goktepe: 2006.
[18] Nebesny E, Zyzelewicz D, Motyl I, Libudzis Z. Chocolate and preparation thereof, Polish patent: P-366273 (Patent Application, 2004) (in polish) - in press:
2012.
[19] KHosrsvi Zanjani M.A, ghiyasi tarzi B, SHarifan A, Mohamadi N, Bakhoda H.
Survival of Bifidobacterium bifidum Encapsulation in water-based cake brain cream: Food Technology & Nutrition 2013; 10 (3) p29-39 [in Persian].
ptimization of probiotic breakfast chocolate formulation
MostafaLou, H. 1, Karazhyan, R. 2*, Ehtiati, A. 3
1. MSc. Graduated Student, Department of Food Science and Technology, ACECR Kashmar Higher Education Institue.
2. Assistant Professor, Department of industrial microbial biotechnology, Iranian Academic Center for Education Culture and Research (ACECR), Mashhad, Iran.
3. Member of faculty, Department of Food quality and safety, Iranian Academic Center for Education Culture and Research (ACECR), Mashhad, Iran.
ABSTRACT ARTIClE INFO
Article History:
Received 2021/ 01/ 31 Accepted 2021/ 10/ 25
Keywords:
Lactobacillus plantarum, Tissue stiffness,
Chocolate, Guar gum, Texture analysis.
Chocolate is one of the most delicious foods, one of the most popular foods that by increasing the variety of breakfast meals, can increase the desire of people to eat breakfast and thus reduce their fatigue and lethargy during the day. Probiotics are microorganisms that, if used in large numbers and live, have health- promoting effects on the host. According to the need of consumers to produce health food products, the purpose of this study is to investigate gum Arabic, guar and lecithin on the quality characteristics and survival of probiotic bacteria in probiotic breakfast chocolate. For this purpose, based on Box-Behnken statistical design, 18 samples were prepared. After complete mixing of compounds (including honey, Tahini, grape juice, cardamom, gum arabic, guar and lecithin) and achieving a uniform texture, the Lactobacillus plantarum probiotic bacteria In the last step, 108 per gram of added and finally, breakfast chocolate was prepared and stored in a cool and dry place. The tests included viscosity, tissue stiffness and probiotic bacteria count. After designing the experiments, regression modeling with feedback removal algorithm, numerical optimization and drawing response surface diagrams were performed with Design-Expert software. The results showed that the addition of guar gum and lecithin had a significant effect on tissue properties (tissue firmness and viscosity) and survival of the probiotic bacterium Lactobacillus plantarum, while the addition of gum arabic and listin had a significant effect on tissue firmness, viscosity and significant increase in The probiotic bacterium did not have Lactobacillus plantarum. Optimal conditions in this study with total desirability, according to the relevant statistical design at a concentration of 0.546 listin, 0.8 guar gum and 0.064 arabic gum, the highest survival of probiotic bacteria was 10.599 cfu / gr and tissue stiffness was 8.22 N.
DOI: 10.52547/fsct.18.120.6
DOR: 20.1001.1.20088787.1400.18.120.6.8
*Corresponding Author E-Mail:
Iranian Journal of Food Science and Technology
Homepage:www.fsct.modares.ir Scientific Research
